CN113686840A - Saliva lactic acid detection device based on chemiluminescence method - Google Patents

Saliva lactic acid detection device based on chemiluminescence method Download PDF

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Publication number
CN113686840A
CN113686840A CN202110971612.5A CN202110971612A CN113686840A CN 113686840 A CN113686840 A CN 113686840A CN 202110971612 A CN202110971612 A CN 202110971612A CN 113686840 A CN113686840 A CN 113686840A
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box
box body
sampling
fixedly connected
motor
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李文满
唐俊铨
胡童宜
李骏
陈琴怡
王婷
罗麟梅
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Chongqing Medical and Pharmaceutical College
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Chongqing Medical and Pharmaceutical College
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76Chemiluminescence; Bioluminescence
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045Devices for taking samples of body liquids
    • A61B10/0051Devices for taking samples of body liquids for taking saliva or sputum samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Pulmonology (AREA)
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  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
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  • Molecular Biology (AREA)
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  • Animal Behavior & Ethology (AREA)
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Abstract

The utility model provides a saliva lactic acid detection device based on chemiluminescence method, is including the box with install four wheels in the box bottom surface, installs sampling unit and detecting element in the box, sampling unit is including the carousel, and the eccentric department of carousel has a plurality of first spouts around the even array in centre of a circle, and the equal slip is provided with sample box subassembly in the first spout, still is provided with the promotion subassembly that can release first spout with sample box subassembly on the carousel, and the box top surface is provided with the sample connection that can instil into sample box subassembly with saliva, the device is still including stirring centrifugation unit, and stirring centrifugation unit can be respectively for the sampling tube stirring or centrifugation. The device can detect the salivary lactic acid in batch and real time, is convenient to use, prevents training damage caused by overlarge exercise intensity of athletes, and avoids errors of detection parameters caused by errors of front and back comparison of samples due to the fact that samples of different personnel are placed in different groups.

Description

Saliva lactic acid detection device based on chemiluminescence method
Technical Field
The invention relates to the technical field of medical examination, in particular to a saliva lactic acid detection device based on a chemiluminescence method.
Background
Physical training is the most common practice for athletes and the most basic military training for troops. In the training process, because the human body is not suitable for the motion and the motion intensity is too big, and cause the motion damage or train the damage, seriously influenced sportsman's physical function and match performance, simultaneously, also endangered army's construction and fighting ability, how to prevent and treat or reduce the training injury, the important realistic problem who needs to solve urgently.
The key point of prevention training injury lies in a scientific training method, and the establishment of the scientific training method depends on accurate grasp of physical functions of the trainees, especially on objective evaluation of physical fatigue degrees of the trainees.
Lactic acid is the product of glycolysis of sugar under anaerobic condition, muscle fatigue is closely related with the lactic acid accumulation, through the lactic acid content before and after the strenuous exercise of survey trainee, can understand the metabolic condition of muscle lactic acid, and then make the judgement to trainee's fatigue strength, lactic acid detection method commonly used is including blood lactic acid detection and saliva lactic acid detection, compare in blood lactic acid detection, saliva lactic acid detects to be the detection of non-invasive, it is more convenient, can not bring the body discomfort for the personnel that need frequently detect, also avoid the cross infection that the blood sampling caused probably.
The existing saliva lactic acid detection methods mainly comprise a liquid chromatography method, a spectrophotometer method, a fluorescence method, a colorimetric method, a chemiluminescence method and the like, and a lactic acid detection system is formed based on the methods, but all the systems need special analysis equipment and are completed in a fixed biochemical analysis chamber by professionals, so that the operation is complex, the efficiency is low, and the rapid detection in batch and real time in the field can not be met when the physical training of trained personnel is carried out.
There is a need for a device for detecting lactic acid in saliva that can detect rapidly.
Disclosure of Invention
The invention aims to provide a saliva lactic acid detection device based on a chemiluminescence method.
The invention aims to realize the technical scheme, which comprises a box body and four wheels arranged on the bottom surface of the box body, wherein a sampling unit and a detection unit are arranged in the box body, the sampling unit comprises a rotary table which is rotatably arranged in the box body, a plurality of first sliding grooves are uniformly arrayed at the eccentric position of the rotary table around the circle center, the length directions of the first sliding grooves are coincided with the circle center of the rotary table, sampling box assemblies are arranged in the first sliding grooves in a sliding manner, a pushing assembly capable of pushing the sampling box assemblies out of the first sliding grooves is further arranged on the rotary table, and a sampling port capable of dripping saliva into the sampling box assemblies is arranged on the top surface of the box body;
the sampling box assemblies respectively comprise more than two sampling tubes, and the sampling tubes can respectively move to the lower parts of the sampling ports under the pushing of the pushing modules;
the device also comprises a stirring centrifugal unit, and the stirring centrifugal unit can be used for stirring or centrifuging the sampling tube respectively.
Preferably, the bottom surface in the box body is fixedly connected with a base with an open top surface, the turntable is rotatably arranged on the base through a bearing, a first motor is arranged in the base, and an output shaft of the first motor is fixedly connected with the circle center position of the bottom surface of the turntable.
Preferably, the sampling box subassembly all includes the box body, and the sampling tube is all through the rotatable setting of bearing in the box body, and arranges according to first spout length direction, and sampling tube one end is opened and is stretched out the box body top surface, and the other end stretches out box body bottom surface and rigid coupling has first gear, is provided with the grid of cutting apart into upper and lower section with the sampling tube in the sampling tube, and a plurality of slidable lids of opening or closing the sampling tube are installed to the box body top surface, just lid and sampling tube one-to-one have all seted up the spacing groove on the box body both sides wall.
Preferably, the pushing assembly comprises a second motor, the second motor is installed on the top surface of the rotary table, an output shaft of the second motor is perpendicular to the ground and fixedly connected to a disc, a plurality of first hinge rods in one-to-one correspondence with the sampling box assemblies are hinged to the outer circumferential wall of the disc, the other ends of the first hinge rods are hinged to first sliding blocks, the first sliding blocks are slidably located in first sliding grooves, and two limiting arms capable of being inserted into limiting grooves are fixedly connected to the first sliding blocks.
Preferably, the centrifugal stirring unit comprises an arc-shaped tooth section which can be meshed with the first gear, the arc-shaped tooth section is slidably arranged on the inner side wall of the box body, the sliding direction of the arc-shaped tooth section is vertical to the bottom surface of the box body, a vertical plate is fixedly connected to the bottom surface of the box body, the vertical plate is positioned between the inner side wall of the box body and the base and is vertical to the ground, the motor mounting seat is slidably arranged on the vertical plate, the sliding direction of the vertical plate is vertical to the bottom surface of the box body, a third motor is arranged on the motor mounting seat, and a second gear which can be meshed with the first gear is fixedly connected to an output shaft of the third motor;
the bottom surface rigid coupling of motor mount pad has the one end of second hinge pole, and the second hinge pole other end and arc tooth section bottom surface rigid coupling, second hinge pole middle part articulate on the bottom surface in the box, still install electric telescopic handle on the bottom surface in the box, electric telescopic handle's flexible end and arc tooth section bottom surface rigid coupling.
Preferably, the detection unit comprises an electric control clamping hand capable of clamping a cotton ball in the sampling pipe, a first spray head capable of adding a chemiluminescent reagent into the sampling pipe, a lifting detection platform capable of lifting and going out of the box body is arranged on the lifting detection platform, a clamping groove used for clamping a mobile phone is formed in the lifting detection platform, a detection hole for a mobile phone camera to shoot a detection hole in the box body is formed in the clamping groove, a first tank body provided with the chemiluminescent reagent is fixedly connected to the top surface of the box body, the bottom of the first tank body is communicated with the first spray head, and the electric control clamping hand, the first spray head and the lifting detection platform are sequentially installed on the top wall of the inner side of the box body along the motion track of the sampling pipe and are located behind the stroke of the stirring centrifugal unit.
Preferably, the bottom surface in the box body is provided with a cotton ball recovery box with an open top surface, the cotton ball recovery box is positioned below the electric control clamp, the side wall of the box body is provided with a hinge door for taking out the cotton ball recovery box, two inner side walls of the cotton ball recovery box, which are close to the top surface, are provided with second sliding chutes, the box cover is slidably arranged in the two second sliding chutes, the sliding direction corresponds to the rotating direction of the turntable, springs are arranged in the second sliding chutes, one end of each spring is fixedly connected with the bottom of the second sliding chute, and the other end of each spring is fixedly connected with the box cover;
the top surface of the box cover is fixedly connected with a first mounting seat and a second mounting seat, the first rotating rod is rotatably mounted between the first mounting seat and the second mounting seat, a lug is fixedly connected to the outer wall of the circumference of the first rotating rod, two ends of the first rotating rod are both sleeved with a torsion spring and fixedly connected with one end of the torsion spring, and the other end of the torsion spring is fixedly connected with the first mounting seat and the second mounting seat respectively;
the bottom surface of the rotary disc is fixedly connected with a plurality of pushing blocks which correspond to the sampling box assemblies one by one, and the pushing blocks can be in contact with the convex blocks.
Preferably, the top surface of the box body is fixedly connected with a second tank body filled with saliva preservation liquid, the bottom surface of the second tank body is provided with a second spray head used for adding the saliva preservation liquid into the sampling pipe, and the second spray head penetrates through the top wall of the box body, extends into the box body, is positioned above the movement track of the sampling pipe and is positioned between the sampling port and the stroke of the stirring centrifugal unit;
a roller component for closing the corresponding box cover is arranged between the second spray head and the stroke of the stirring centrifugal unit, a roller component for opening the corresponding box cover is arranged between the stroke of the stirring centrifugal unit and the stroke of the detection unit, and a roller component for opening or closing the corresponding box cover is arranged between the stroke of the detection unit and the stroke of the sampling port;
the roller component comprises a fourth motor, the fourth motor is installed on the top wall of the inner side of the box body, an output shaft of the fourth motor is fixedly connected with an eccentric roller which can be in contact with the box cover, and the eccentric roller is located above the motion trail of the sampling tube.
Due to the adoption of the technical scheme, the invention has the following advantages:
1. the saliva lactic acid detection device can detect saliva lactic acid in batches and in real time, is convenient to use, prevents training damage caused by overlarge exercise intensity of athletes, prevents samples of different personnel from being placed in different groups, avoids error of detection parameters caused by error of front and back comparison of the samples, has two paths for preparation of the detected samples, can prepare the samples normally when the saliva is sufficient, chews a cotton ball when the saliva is insufficient, centrifuges the cotton ball, prepares the saliva samples after obtaining the saliva samples, and is simple in structure and high in efficiency.
2. The pushing assembly can push all sampling box assemblies to slide simultaneously, the sampling tubes of different gears are adjusted to be located below the sampling ports, first gears below different sampling tubes can be matched with the stirring centrifugal unit, the stirring centrifugal unit moves through the electric telescopic rod, and the arc-shaped tooth section and the first gears below the second gears and the sampling tubes are selected to be meshed with each other to obtain different processing effects.
3. The rotary table rotates to drive different sampling boxes to be sequentially processed, the box cover on the cotton ball recovery box is also driven to be opened in the rotating process, the cotton balls are conveniently recovered, when the cotton balls are not detected, the cotton ball recovery box is closed by the box cover, the sampling tubes are closed by the box cover, each space is relatively closed, the virus propagation risk is reduced,
additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof.
Drawings
The drawings of the present invention are described below.
Fig. 1 is an overall schematic view of the present invention.
Fig. 2 is a schematic view of the turntable of the present invention.
FIG. 3 is a schematic view of a sample block assembly of the present invention.
FIG. 4 is a schematic view of a stirred centrifuge unit of the present invention.
Fig. 5 is a schematic view of a base of the present invention.
FIG. 6 is an internal schematic view of the present invention.
FIG. 7 is a schematic view of a detecting unit according to the present invention.
FIG. 8 is a schematic view of the cotton ball recycling bin of the present invention.
Fig. 9 is a schematic view of a pusher block of the present invention.
In the figure: 1. a box body; 2. a wheel; 3. a turntable; 4. a first chute; 5. a sampling port; 6. a sampling tube; 7. a base; 8. a first motor; 9. a box body; 10. a first gear; 11. a grid; 12. a box cover; 13. a limiting groove; 14. a second motor; 15. a disc; 16. a first hinge rod; 17. a first slider; 18. a limiting arm; 19. an arc-shaped tooth section; 20. a vertical plate; 21. a motor mounting seat; 22. a third motor; 23. a second gear; 24. a second hinge rod; 25. an electric telescopic rod; 26. electrically controlled clamping hands; 27. a first nozzle; 28. lifting the detection platform; 29. a first tank; 30. a cotton ball recovery box; 31. a hinge door; 32. a second chute; 33. a box cover; 34. a spring; 35. a first mounting seat; 36. a second mounting seat; 37. a first rotating lever; 38. a bump; 39. a torsion spring; 40. a pushing block; 41. a second tank; 42. a second nozzle; 43. a fourth motor; 44. an eccentric roller; 45. a card slot; 46. and (6) detecting holes.
Detailed Description
The invention is further illustrated by the following figures and examples.
In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the embodiments of the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. Specific meanings of the above terms in the embodiments of the present invention can be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1 to 3, a saliva lactic acid detecting device based on a chemiluminescence method comprises a box body 1 and four wheels 2 arranged on the bottom surface of the box body 1, wherein a sampling unit and a detecting unit are arranged in the box body 1, the sampling unit comprises a rotary disc 3 rotatably arranged in the box body 1, a plurality of first sliding grooves 4 are uniformly arrayed at the eccentric position of the rotary disc 3 around the circle center, the length directions of the first sliding grooves 4 are coincided with the circle center of the rotary disc 3, sampling box assemblies are arranged in the first sliding grooves 4 in a sliding manner, pushing assemblies capable of pushing the sampling box assemblies out of the first sliding grooves 4 are further arranged on the rotary disc 3, and a sampling port 5 capable of dropping saliva into the sampling box assemblies is arranged on the top surface of the box body 1;
the sampling box assemblies respectively comprise more than two sampling tubes 6, and the sampling tubes 6 can respectively move to the lower parts of the sampling ports 5 under the pushing of the pushing modules;
the device also comprises a stirring centrifugal unit which can respectively stir or centrifuge the sampling tube 6.
In the embodiment, before the movement starts, the pushing assembly can push the sampling box assembly to slide outwards, the first sampling tube extends out of the rotary table, the rotary table rotates, the sampling tube on the outermost side can be sequentially positioned below the sampling port, an athlete spits saliva into the sampling tube from the sampling port, the rotary table drives the sampling tube to move to the stirring centrifugal unit for processing, and the detection unit detects the initial value of the salivary lactic acid according to the luminous value after adding the chemiluminescent reagent into the sampling tube;
move when the certain degree, the sportsman comes the device once more before, it outwards slides to promote the subassembly to promote the sampling box subassembly once more, the carousel is stretched out to the second sampling tube, the carousel rotates, this sampling tube that keeps off the position can be located the thief hatch below in proper order, the sportsman is in spitting saliva into the thief hatch from the thief hatch, the carousel drives the sampling tube and moves and handle to stirring centrifugal unit, detecting element adds the chemiluminescence reagent after to the sampling tube, detect out the salivary lactic acid numerical value this moment according to the luminous value, learn sportsman's motion intensity according to salivary lactic acid numerical value, prevent that sportsman's motion intensity is too big, cause the training damage.
As shown in fig. 5, the bottom surface in the box body 1 is fixedly connected with a base 7 with an open top surface, the turntable 3 is rotatably installed on the base 7 through a bearing, a first motor 8 is installed in the base 7, and an output shaft of the first motor 8 is fixedly connected with the center of a circle of the bottom surface of the turntable 3.
In this embodiment, the turntable is driven to rotate by a first motor.
As shown in fig. 3, the sampling box subassembly all includes box body 9, and sampling tube 6 all is in box body 9 through the rotatable setting of bearing, and arranges according to 4 length direction of first spout, and sampling tube 6 one end is opened and is stretched out box body 9 top surface, and the other end stretches out box body 9 bottom surface and rigid coupling has first gear 10, is provided with in the sampling tube 6 and splits into the grid 11 of upper and lower section with sampling tube 6, and box body 9 top surface is installed a plurality of slidable and is opened or close lid 12 of sampling tube 6, just lid 12 and sampling tube 6 one-to-one, and spacing groove 13 has all been seted up on the wall of box body 9 both sides.
In the embodiment, the sampling tubes are arranged in the length direction of the first sliding chute, the sampling tube at the outermost side is used for collecting initial saliva, the sampling tube at the back is used for collecting subsequent saliva, when an athlete moves to a certain degree, the saliva is sticky and foams are increased due to the loss of a large amount of sweat, the saliva is difficult to spit out, the cotton ball can be placed into the sampling tubes by chewing cotton balls, cotton swabs and other articles, the grid can block the cotton ball, and the cotton ball is prevented from falling to the bottom in the sampling tube;
the sampling tube in the outside adds the preservative fluid after collecting the saliva, extension saliva save time, stirring centrifugation unit can stir the mixing to it, the sampling tube of rear side is after collecting the saliva cotton ball, also need to add the preservative fluid, preservative fluid and saliva cotton ball all are located the sampling tube, the saliva centrifugation of stirring centrifugation unit in with the saliva cotton ball comes out, fall to the sampling tube lower half, the cotton ball in the sampling tube is taken out again to the detecting element, follow-up operation is unanimous with all the other sampling tubes of unused cotton ball.
As shown in fig. 2, the pushing assembly includes a second motor 14, the second motor 14 is installed on the top surface of the turntable 3, an output shaft of the second motor 14 is perpendicular to the ground, and a disk 15 is fixedly connected to the output shaft, a plurality of first hinge rods 16 corresponding to the sampling box assemblies one by one are hinged to the outer circumferential wall of the disk 15, the other ends of the first hinge rods 16 are respectively hinged to a first sliding block 17, the first sliding block 17 is slidably located in the first sliding groove 4, and two limiting arms 18 capable of being inserted into the limiting grooves 13 are fixedly connected to the first sliding block 17.
In this embodiment, the second motor rotates to drive the first hinge rod to rotate, the first hinge rod pushes the first sliding block to slide, and the first sliding block drives each sampling box component to slide at the same time.
As shown in fig. 4, the centrifugal stirring unit includes an arc-shaped tooth section 19 capable of meshing with the first gear 10, the arc-shaped tooth section 19 is slidably disposed on an inner side wall of the box body 1, and a sliding direction is perpendicular to a bottom surface of the box body 1, a vertical plate 20 is further fixedly connected to the bottom surface of the box body 1, the vertical plate 20 is located between the inner side wall of the box body 1 and the base 7 and is perpendicular to the ground, a motor mounting seat 21 is slidably mounted on the vertical plate 20, and the sliding direction is perpendicular to the bottom surface of the box body 1, a third motor 22 is mounted on the motor mounting seat 21, and a second gear 23 capable of meshing with the first gear 10 is fixedly connected to an output shaft of the third motor 22;
the bottom surface of motor mount pad 21 is fixed with the one end of second hinge rod 24, and the second hinge rod 24 other end and the 19 bottom surfaces of arc tooth section rigid couplings, and second hinge rod 24 middle part articulates on the bottom surface in box 1, still installs electric telescopic handle 25 on the bottom surface in box 1, and electric telescopic handle 25's flexible end and the 19 bottom surfaces of arc tooth section rigid couplings.
In the embodiment, when the second hinge rod is level, the arc-shaped tooth section and the second gear are both lower than the plane of the turntable and are not in contact with the first gear below the sampling tube, and the sampling box assembly can freely pass through the centrifugal stirring unit;
when the electric telescopic rod is ejected out, the arc-shaped tooth section rises, the second gear falls, the first gear on the outermost side is meshed with the arc-shaped tooth section, and when the turntable rotates, the arc-shaped tooth section drives the sampling tube to rotate to stir, uniformly mix and preserve the liquid for the sampling tube on the outermost side;
when the electric telescopic rod descends, the arc-shaped tooth section descends, the second gear ascends, the first gear on the rear side is meshed with the second gear, the third motor can rotate at a low speed and is used for stirring, uniformly mixing and preserving fluid for the sampling tube at the position, if a cotton ball is used, the third motor can rotate at a high speed to drive the sampling tube to rotate at a high speed and is used for centrifuging the sampling tube at the position, so that saliva in the cotton ball is separated into the sampling tube, and the cotton ball can be taken out subsequently;
after centrifugal stirring centrifugal work accomplished, electric telescopic handle descends, and arc-shaped tooth section and second gear resume to flush, are less than the carousel plane, dodge out first gear, all not all with first gear contact, the carousel can drive the sample box subassembly and leave centrifugal stirring unit.
As shown in fig. 5 to 7, the detection unit includes an electric control gripper 26 capable of gripping a cotton ball in the sampling tube 6, a first nozzle 27 capable of adding a chemiluminescent reagent into the sampling tube 6, a lifting detection platform 28 capable of lifting into and out of the box body 1, a slot 45 for clamping a mobile phone is provided on the lifting detection platform 28, a detection hole 46 for a mobile phone camera to shoot the box body 1 is provided in the slot 45, a first tank 29 containing the chemiluminescent reagent is fixedly connected to the top surface of the box body 1, the bottom of the first tank 29 is communicated with the first nozzle 27, and the electric control gripper 26, the first nozzle 27 and the lifting detection platform 28 are sequentially installed on the inner top wall of the box body 1 along the movement track of the sampling tube 6 and are located after the stroke of the stirring centrifugal unit.
In the embodiment, when the sampling tube at the outermost side moves to the detection unit, the electric control gripper does not work to avoid the sampling tube, the first spray head drops a chemiluminescent reagent into the sampling tube, the mobile phone is inserted into the clamping groove of the lifting detection platform in advance, the camera of the mobile phone is positioned in the detection hole and can be arranged in the shooting box, the lifting detection platform descends, the camera of the mobile phone is aligned to the sampling tube, at the moment, a darkroom is formed in the box, the camera is matched with a special detection APP on the mobile phone to identify the luminous value in the sampling tube, the APP evaluates the lactic acid value according to the luminous reaction and can be shared to other terminals, so that coaches and medical staff can master the conditions of athletes at the first time, and the data can be carried, transmitted and shared more conveniently through the mobile phone;
when the sampling tube of rear side moved to the detecting element, if follow-up sampling tube used the cotton ball, automatically controlled tong then stretched into the sampling tube and pressed from both sides out the cotton ball, and first shower nozzle instils into the chemiluminescence reagent to the sampling tube, and lift testing platform descends, aligns the sampling tube with the camera of cell-phone, and APP assesses the lactic acid value for contrast salivary lactic acid value difference.
As shown in fig. 8 to 9, a cotton ball recycling box 30 with an open top surface is installed on the bottom surface in the box body 1, the cotton ball recycling box 30 is located below the electric control clamping hand 26, a hinge door 31 for taking out the cotton ball recycling box 30 is opened on the side wall of the box body 1, second chutes 32 are opened on two inner side walls of the cotton ball recycling box 30 close to the top surface, a box cover 33 is slidably installed in the two second chutes 32, the sliding direction corresponds to the rotating direction of the turntable 3, a spring 34 is installed in the second chute 32, one end of the spring 34 is fixedly connected with the bottom of the second chute 32, and the other end of the spring 34 is fixedly connected with the box cover 33;
a first mounting seat 35 and a second mounting seat 36 are fixedly connected to the top surface of the box cover 1, a first rotating rod 37 is rotatably mounted between the first mounting seat 35 and the second mounting seat 36, a convex block 38 is fixedly connected to the outer circumferential wall of the first rotating rod 37, two ends of the first rotating rod 37 are respectively sleeved with a torsion spring 39 and fixedly connected with one end of the torsion spring 39, and the other end of the torsion spring 39 is fixedly connected with the first mounting seat 35 and the second mounting seat 36 respectively;
the bottom surface of the rotary disc 3 is fixedly connected with a plurality of pushing blocks 40 which correspond to the sampling box assemblies one by one, and the pushing blocks 40 can be contacted with the convex blocks 38.
In the embodiment, when the rotary disc rotates to the cotton ball recovery box, the pushing block is contacted with the convex block, and in an initial state, the force of the torsion spring is larger than that of the spring, so that the pushing block pushes the convex block, the convex block drives the box cover to slide and open in the second sliding groove, the spring is compressed, and the electric control gripper clamps the cotton balls out of the sampling tube;
the carousel continues to rotate, automatically controlled tong is staggered to the sampling tube, automatically controlled tong loosens the cotton ball, the cotton ball falls into the cotton ball collection box that the below was opened, the carousel resumes to rotate again, move to extreme position in the case lid second spout, unable continuation motion pushes away the piece and continues to promote the lug, the lug begins to rotate, drive first pivot pole and rotate, the torsional spring is twistd reverse, cross after the lug rotates and push away the piece, the torsional spring resets, the carousel continues to move forward, the spring is closed the case lid.
As shown in fig. 5 to 7, a second tank 41 filled with saliva preservation solution is fixedly connected to the top surface of the box 1, a second spray head 42 for adding saliva preservation solution into the sampling tube 6 is installed on the bottom surface of the second tank 41, and the second spray head 42 penetrates through the top wall of the box 1, extends into the box 1, is located above the movement track of the sampling tube 6, and is located between the sampling port 5 and the stroke of the stirring centrifugal unit;
a roller component for closing the corresponding box cover 12 is arranged between the second spray head 42 and the stroke of the stirring centrifugal unit, a roller component for opening the corresponding box cover 12 is arranged between the stroke of the stirring centrifugal unit and the stroke of the detection unit, and a roller component for opening or closing the corresponding box cover 12 is arranged between the stroke of the detection unit and the sampling port 5;
the roller components comprise a fourth motor 43, the fourth motor 43 is installed on the top wall of the inner side of the box body 1, and an output shaft of the fourth motor 43 is fixedly connected with an eccentric roller 44 which can be in contact with the box cover 12 and is positioned above the motion trail of the sampling tube 6.
In the embodiment, in an initial state, the roller assembly between the detection unit and the sampling port is used for opening the box cover on the sampling tube and collecting saliva after the sampling tube collects saliva, the sampling tube rotates to the second spray head to add preservation liquid, the roller assembly between the second spray head and the stirring centrifugal unit can close the box cover, the stirring centrifugal unit processes the sampling tube and then rotates to the detection unit, the roller assembly between the stirring centrifugal unit and the detection unit opens the box cover of the sampling tube again and adds a chemiluminescent reagent for detection, and after the detection is finished, the roller assembly between the detection unit and the sampling port is also used for closing the box cover to complete a cycle, the pushing unit can move and is switched to a next sampling tube;
the fourth motor rotates to drive the eccentric rolling shaft to contact with the box cover, the box cover can be opened when the rotating direction of the eccentric rolling shaft is opposite to that of the rotary plate, and the box cover can be closed when the rotating direction of the eccentric rolling shaft is the same as that of the rotary plate.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the embodiments of the invention without departing from the spirit and scope of the invention, which is to be covered by the claims.

Claims (8)

1. A saliva lactic acid detection device based on a chemiluminescence method comprises a box body (1) and four wheels (2) arranged on the bottom surface of the box body (1), wherein a sampling unit and a detection unit are arranged in the box body (1), and the saliva lactic acid detection device is characterized in that the sampling unit comprises a rotary disc (3) rotatably arranged in the box body (1), a plurality of first sliding grooves (4) are uniformly arrayed at the eccentric position of the rotary disc (3) around the circle center, the length direction of each first sliding groove (4) is coincided with the circle center of the rotary disc (3), a sampling box assembly is arranged in each first sliding groove (4) in a sliding manner, a pushing assembly capable of pushing the sampling box assembly out of the first sliding groove (4) is further arranged on the rotary disc (3), and a sampling port (5) capable of dropping saliva into the sampling box assembly is arranged on the top surface of the box body (1);
the sampling box assembly comprises more than two sampling tubes (6), and the sampling tubes (6) can respectively move to the lower parts of the sampling ports (5) under the pushing of the pushing module;
the device also comprises a stirring centrifugal unit which can be used for stirring or centrifuging the sampling tube (6) respectively.
2. The device for detecting lactic acid in saliva based on the chemiluminescence method according to claim 1, wherein a base (7) with an open top surface is fixedly connected to the inner bottom surface of the box body (1), the turntable (3) is rotatably mounted on the base (7) through a bearing, a first motor (8) is mounted in the base (7), and an output shaft of the first motor (8) is fixedly connected to the center of the bottom surface of the turntable (3).
3. The device for detecting the salivary lactic acid based on the chemiluminescence method as claimed in claim 1, wherein the sampling box assemblies each comprise a box body (9), the sampling tubes (6) are rotatably arranged in the box body (9) through bearings and are arranged in the length direction of the first chute (4), one end of each sampling tube (6) is open and extends out of the top surface of the box body (9), the other end of each sampling tube (6) extends out of the bottom surface of the box body (9) and is fixedly connected with a first gear (10), a grid (11) for dividing the sampling tube (6) into upper and lower sections is arranged in each sampling tube (6), a plurality of box covers (12) capable of opening or closing the sampling tubes (6) in a sliding manner are arranged on the top surface of the box body (9), the box covers (12) correspond to the sampling tubes (6) one to one, and limit grooves (13) are formed in two side walls of the box body (9).
4. The device for detecting the salivary lactic acid based on the chemiluminescence method as claimed in claim 1, wherein the pushing assembly comprises a second motor (14), the second motor (14) is mounted on the top surface of the rotating disk (3), the output shaft of the second motor (14) is perpendicular to the ground, a disk (15) is fixedly connected to the output shaft, a plurality of first hinge rods (16) corresponding to the sampling box assemblies one by one are hinged to the outer circumferential wall of the disk (15), the other ends of the first hinge rods (16) are hinged to first sliding blocks (17), the first sliding blocks (17) are slidably located in the first sliding grooves (4), and two limiting arms (18) capable of being inserted into the limiting grooves (13) are fixedly connected to the first sliding blocks (17).
5. The device for detecting the lactic acid in the saliva based on the chemiluminescence method according to claim 1, wherein the centrifugal stirring unit comprises an arc-shaped tooth section (19) capable of meshing with the first gear (10), the arc-shaped tooth section (19) is slidably disposed on the inner side wall of the box body (1) and has a sliding direction perpendicular to the bottom surface of the box body (1), a vertical plate (20) is further fixedly connected to the inner bottom surface of the box body (1), the vertical plate (20) is located between the inner side wall of the box body (1) and the base (7) and perpendicular to the ground, the motor mounting seat (21) is slidably mounted on the vertical plate (20) and has a sliding direction perpendicular to the bottom surface of the box body (1), the motor mounting seat (21) is provided with a third motor (22), and a second gear (23) capable of meshing with the first gear (10) is fixedly connected to an output shaft of the third motor (22);
the bottom surface rigid coupling of motor mount pad (21) has the one end of second hinge pole (24), and second hinge pole (24) other end and arc tooth section (19) bottom surface rigid coupling, and second hinge pole (24) middle part articulates on box (1) bottom surface, still installs electric telescopic handle (25) on the bottom surface in box (1), and electric telescopic handle (25)'s flexible end and arc tooth section (19) bottom surface rigid coupling.
6. The device for detecting the salivary lactic acid based on the chemiluminescence method according to claim 1, wherein the detection unit comprises an electric control gripper (26) capable of gripping a cotton ball in the sampling tube (6), a first spray head (27) capable of adding a chemiluminescence reagent into the sampling tube (6), a lifting detection platform (28) capable of lifting and moving in and out of the box body (1), a clamping groove (45) for clamping a mobile phone is formed in the lifting detection platform (28), a detection hole (46) for shooting the mobile phone camera in the box body (1) is formed in the clamping groove (45), a first tank body (29) filled with the chemiluminescence reagent is fixedly connected to the top surface of the box body (1), the bottom of the first tank body (29) is communicated with the first spray head (27), and the electric control gripper (26), the first spray head (27) and the lifting detection platform (28) are sequentially installed on the top wall of the inner side of the box body (1) along the movement track of the sampling tube (6), and is located after the stroke of the agitating centrifugal unit.
7. The device for detecting the salivary lactic acid based on the chemiluminescence method according to claim 1, wherein a cotton ball recovery box (30) with an open top surface is mounted on the inner bottom surface of the box body (1), the cotton ball recovery box (30) is located below the electrically controlled gripper (26), a hinge door (31) capable of taking out the cotton ball recovery box (30) is formed in the side wall of the box body (1), second sliding grooves (32) are formed in two inner side walls, close to the top surface, of the cotton ball recovery box (30), box covers (33) are slidably mounted in the two second sliding grooves (32), the sliding direction corresponds to the rotation direction of the turntable (3), springs (34) are mounted in the second sliding grooves (32), one ends of the springs (34) are fixedly connected with the bottoms of the second sliding grooves (32), and the other ends of the springs are fixedly connected with the box covers (33);
the top surface of the box cover (1) is fixedly connected with a first mounting seat (35) and a second mounting seat (36), a first rotating rod (37) is rotatably mounted between the first mounting seat (35) and the second mounting seat (36), a convex block (38) is fixedly connected to the outer wall of the circumference of the first rotating rod (37), two ends of the first rotating rod (37) are sleeved with a torsion spring (39) and fixedly connected with one end of the torsion spring (39), and the other end of the torsion spring (39) is fixedly connected with the first mounting seat (35) and the second mounting seat (36) respectively;
the bottom surface of the rotary disc (3) is fixedly connected with a plurality of pushing blocks (40) which correspond to the sampling box assemblies one by one, and the pushing blocks (40) can be in contact with the convex blocks (38).
8. The device for detecting the salivary lactic acid based on the chemiluminescence method as claimed in claim 1, wherein a second tank (41) filled with a saliva preserving fluid is fixedly connected to the top surface of the box body (1), a second spray head (42) for adding the saliva preserving fluid into the sampling tube (6) is mounted on the bottom surface of the second tank (41), and the second spray head (42) penetrates through the top wall of the box body (1) to extend into the box body (1) and is positioned above the movement track of the sampling tube (6) and between the sampling port (5) and the stroke of the stirring centrifugal unit;
a roller component for closing the corresponding box cover (12) is arranged between the second spray head (42) and the stroke of the stirring centrifugal unit, a roller component for opening the corresponding box cover (12) is arranged between the stroke of the stirring centrifugal unit and the stroke of the detection unit, and a roller component for opening or closing the corresponding box cover (12) is arranged between the stroke of the detection unit and the sampling port (5);
the roller component comprises a fourth motor (43), the fourth motor (43) is installed on the top wall of the inner side of the box body (1), an output shaft of the fourth motor (43) is fixedly connected with an eccentric roller (44) which can be in contact with the box cover (12), and the eccentric roller (44) is located above the motion trail of the sampling tube (6).
CN202110971612.5A 2021-08-24 2021-08-24 Saliva lactic acid detection device based on chemiluminescence method Withdrawn CN113686840A (en)

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CN202110971612.5A CN113686840A (en) 2021-08-24 2021-08-24 Saliva lactic acid detection device based on chemiluminescence method

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Application Number Priority Date Filing Date Title
CN202110971612.5A CN113686840A (en) 2021-08-24 2021-08-24 Saliva lactic acid detection device based on chemiluminescence method

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114112540A (en) * 2021-12-24 2022-03-01 蚌埠学院 Online quick quality of water residual chlorine analyzer
CN114910657A (en) * 2022-07-15 2022-08-16 山东第一医科大学第一附属医院(山东省千佛山医院) Anal secretion detection device
CN115615754A (en) * 2022-09-05 2023-01-17 苏州大学附属儿童医院 Automatic sampler for sampling tube
CN116990219A (en) * 2023-09-22 2023-11-03 江苏德励达新材料股份有限公司 Polyurethane panel stability sampling detection device

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114112540A (en) * 2021-12-24 2022-03-01 蚌埠学院 Online quick quality of water residual chlorine analyzer
CN114112540B (en) * 2021-12-24 2023-08-29 蚌埠学院 On-line rapid water quality residual chlorine analyzer
CN114910657A (en) * 2022-07-15 2022-08-16 山东第一医科大学第一附属医院(山东省千佛山医院) Anal secretion detection device
CN115615754A (en) * 2022-09-05 2023-01-17 苏州大学附属儿童医院 Automatic sampler for sampling tube
CN115615754B (en) * 2022-09-05 2023-09-19 苏州大学附属儿童医院 Automatic sampler for sampling tube
CN116990219A (en) * 2023-09-22 2023-11-03 江苏德励达新材料股份有限公司 Polyurethane panel stability sampling detection device
CN116990219B (en) * 2023-09-22 2023-11-24 江苏德励达新材料股份有限公司 Polyurethane panel stability sampling detection device

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