CN113670899A - Sample scheduling detection system and method for chemiluminescence immunoassay analyzer - Google Patents
Sample scheduling detection system and method for chemiluminescence immunoassay analyzer Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
Abstract
The invention discloses a sample scheduling and detecting system of a chemiluminescence immunoassay analyzer, which comprises: the sample information acquisition module is used for associating the identification code and the position code of the sample with a sample analysis item corresponding to the sample; the sample information loading module automatically loads a sample analysis item to a certain reagent strip position in a certain reaction bin; the sample adding control module is used for identifying the loaded reaction bin and the reagent strip position and controlling the manipulator to add the sample to the corresponding reagent strip position by using the sample adding head; the sample reaction control module controls the liquid transfer mechanism in the reaction bin to operate so as to complete the uniform mixing and reaction of the sample and the reagent; and the sample detection control module controls the reagent strip in the reaction bin to move to the detection bin, and the detection mechanism in the detection bin is used for performing luminescence test on the substance to be detected on the reagent strip. The system has the advantages of high response speed, good reliability and stability, and capability of improving the reliability, safety and stability of the operation of the analyzer.
Description
Technical Field
The invention relates to the technical field of chemiluminescence immunoassay, in particular to a sample scheduling detection system and method of a chemiluminescence immunoassay analyzer.
Background
Chemiluminescence analysis is an analytical method for determining the content of a substance based on the intensity of radiant light generated by a chemical reaction. The chemiluminescence immune analysis combines a chemiluminescence system with immune reaction, after a substance related to chemiluminescence is used for marking an antibody or an antigen and the like to react with an antigen or an antibody and the like to be detected, a free chemiluminescence marker is separated, other related substances of the chemiluminescence system are added to generate chemiluminescence, and the antigen or the antibody and the like are quantitatively or qualitatively detected.
The chemiluminescence immunoassay technology has high accuracy and specificity and is one of important technologies in the detection method. Chemiluminescence immunoassay technology is widely used in-vitro diagnosis experiments in the aspects of body immune function, infectious diseases, endocrine function, tumor markers, sex hormones, thyroid function and the like as a main means for disease diagnosis.
The chemiluminescence immunoassay is carried out by adopting an automatic immunoassay analyzer; the automatic analysis process of the automatic immunity analyzer is realized by a control system; the reliability, response speed and stability of the control system directly influence the operational reliability and analysis accuracy of the chemiluminescence immunoassay analyzer.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a system and a method for dispatching and detecting a sample of a chemiluminescence immunoassay analyzer, wherein the system has the advantages of high response speed, good reliability and stability, and capability of improving the reliability, safety and stability of the operation of the analyzer.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
a chemiluminescent immunoassay analyzer sample dispatching detection system comprises a sample bin, a consumable bin, a reaction bin and a detection bin, wherein the sample bin is internally provided with a sample rack, a sample is placed on the sample rack, the consumable bin is internally provided with a sample adding head, and the reaction bin is internally provided with a reagent strip and a liquid transferring mechanism; the sample scheduling system includes:
the sample information acquisition module is used for scanning the identification code of the sample and the position code on the sample rack where the sample is positioned by using the sample code scanner and associating the identification code and the position code with the sample analysis item corresponding to the sample;
the sample information loading module automatically loads a sample analysis item to a certain reagent strip position in a certain reaction bin;
the sample adding control module is used for identifying the reaction bin and the reagent strip position loaded by the sample information loading module, controlling the manipulator to grab an empty sample adding head, enabling the manipulator to move the sample adding head to the corresponding sample analysis item position to extract a sample, and then controlling the manipulator to add the sample to the corresponding reagent strip position;
the sample reaction control module controls the liquid transfer mechanism in the reaction bin to operate so as to complete the uniform mixing and reaction of the sample and the reagent;
and the sample detection control module controls the reagent strip in the reaction bin to move to the detection bin, and the detection mechanism in the detection bin is used for performing luminescence test on the substance to be detected on the reagent strip.
Furthermore, the sample scheduling detection system further comprises a risk avoiding module for recording the door states of the reaction bins, and when a certain reaction bin is in a sample adding state, the door of the reaction bin relatively close to the sample bin is preset to be in a busy state.
Furthermore, the sample scheduling detection system further comprises a sample type distinguishing module which is connected with the sample detection control module and used for distinguishing the sample type and associating the sample type with the sample detection control module, and the sample detection control module determines the calculation method according to the sample type.
Furthermore, the sample scheduling detection system also comprises a detection result query and display module which is connected with the sample detection control module.
The invention also provides a dispatching detection method of the chemiluminescent immunoassay analyzer sample dispatching detection system, which comprises the following steps:
(1) acquiring an identification code of a sample and a position code of the sample in a sample bin through a sample information acquisition module, and associating the identification code and the position code with a sample analysis item of the corresponding sample;
(2) automatically loading a sample analysis item to a certain reagent strip position in a certain reaction bin through a sample information loading module;
(3) identifying the reaction bin and the reagent strip position loaded by the sample information loading module through the sample loading control module, controlling the manipulator to grab an empty sample loading head, moving the manipulator to the corresponding sample analysis item position to extract a sample, and then controlling the manipulator to load the sample to the corresponding reagent strip position;
(4) controlling a liquid transfer mechanism in the reaction bin to operate through a sample reaction control module so as to complete the uniform mixing and reaction of the sample and the reagent;
(5) the reagent strip in the reaction bin is controlled to move to the detection bin through the sample detection control module, and the detection mechanism in the detection bin is used for carrying out luminescence test on the substance to be detected on the reagent strip.
Further, in the step (3), before the sample filling head fills the sample, the system firstly judges the type of the sample through a sample type judging module, and associates the type of the sample with a sample detection control module, and the sample detection control module determines a calculation method according to the type of the sample.
Further, after the step (5), the system displays the detection result through the detection result query and display module and stores the detection result on the capacitive touch screen for the user to query.
Furthermore, the system also records the door states of all the reaction bins through the risk avoiding module, and when a certain reaction bin is in a sample adding state, the risk avoiding module presets the door of the reaction bin relatively close to the sample bin to be in a busy state so as to prompt a user that the door of the reaction bin cannot be opened; if the user forcibly opens the bin door of the reaction bin which is preset to be in a busy state, the risk avoiding module controls the reaction bin in the sample adding state to stop sample adding.
The invention has the beneficial effects that:
the invention collects sample numbers and sample positions through a sample information collection module, associates corresponding sample analysis items, automatically loads the sample analysis items to a certain reagent strip position in a certain reaction bin through a sample information loading module, identifies the loaded reaction bin and the reagent strip position through a sample loading control module, loads the sample to the corresponding reagent strip position through a manipulator and a sample loading head, controls a liquid transferring mechanism in the reaction bin to operate through the sample reaction control module so as to complete the uniform mixing and reaction of the sample and the reagent, controls the reagent strip in the reaction bin to move to a detection bin through a sample detection control module, and performs a luminescence test on a substance to be detected on the reagent strip through the detection mechanism in the detection bin.
The invention can also judge the sample type through the sample type judging module and associate the sample type to the sample detection control module, and the sample detection control module can determine the calculation method of the detection process according to the sample type.
The invention can also record the door state of each reaction bin through the risk avoiding module, and when a certain reaction bin is in a sample adding state, the risk avoiding module presets the door of the reaction bin relatively close to the sample bin to be in a busy state so as to prompt a user that the door of the reaction bin can not be opened; if the user forces to open the bin door of the reaction bin which is preset to be in a busy state, the risk avoiding module controls the reaction bin in the sample adding state to stop sample adding, and the operation reliability and stability of the analyzer are improved.
The invention controls the chemiluminescence immunity analyzer to automatically sample adding, reacting and detecting processes by matching each module, the system has fast response speed and good reliability and stability, thereby improving the reliability, safety and stability of the operation of the analyzer and realizing the high-efficiency and high-accuracy analysis and detection of samples.
Drawings
Fig. 1 is a schematic block diagram of a sample scheduling detection system of a chemiluminescent immunoassay analyzer according to the present invention.
Detailed Description
The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention easier to understand by those skilled in the art, and thus will clearly and clearly define the scope of the invention.
As shown in fig. 1, a sample dispatching and detecting system of a chemiluminescent immunoassay analyzer comprises a sample bin, a consumable bin, a reaction bin and a detection bin, wherein the sample bin is provided with a sample rack, a sample is placed on the sample rack, the consumable bin is provided with a sample adding head, and the reaction bin is provided with a reagent strip and a liquid transferring mechanism; the sample scheduling system includes:
the sample information acquisition module is used for scanning the identification code of the sample and the position code on the sample rack where the sample is positioned by using the sample code scanner and associating the identification code and the position code with the sample analysis item corresponding to the sample;
the sample information loading module automatically loads a sample analysis item to a certain reagent strip position in a certain reaction bin;
the sample adding control module is used for identifying the reaction bin and the reagent strip position loaded by the sample information loading module, controlling the manipulator to grab an empty sample adding head, enabling the manipulator to move the sample adding head to the corresponding sample analysis item position to extract a sample, and then controlling the manipulator to add the sample to the corresponding reagent strip position;
the sample reaction control module controls the liquid transfer mechanism in the reaction bin to operate so as to complete the uniform mixing and reaction of the sample and the reagent;
and the sample detection control module controls the reagent strip in the reaction bin to move to the detection bin, and the detection mechanism in the detection bin is used for performing luminescence test on the substance to be detected on the reagent strip.
The sample scheduling and detecting system further comprises a risk avoiding module for recording the door states of the reaction bins, and when a certain reaction bin is in a sample adding state, the door of the reaction bin relatively close to the sample bin is preset to be in a busy state.
The sample scheduling detection system also comprises a sample type distinguishing module which is connected with the sample detection control module and used for distinguishing the sample type and associating the sample type to the sample detection control module, and the sample detection control module determines a calculation method according to the sample type.
The sample scheduling detection system also comprises a detection result query and display module which is connected with the sample detection control module.
The scheduling detection method of the sample scheduling detection system of the chemiluminescence immunoassay analyzer comprises the following steps:
(1) acquiring an identification code of a sample and a position code of the sample in a sample bin through a sample information acquisition module, and associating the identification code and the position code with a sample analysis item of the corresponding sample;
(2) automatically loading a sample analysis item to a certain reagent strip position in a certain reaction bin through a sample information loading module;
(3) identifying the reaction bin and the reagent strip position loaded by the sample information loading module through the sample loading control module, controlling the manipulator to grab an empty sample loading head, moving the manipulator to the corresponding sample analysis item position to extract a sample, and then controlling the manipulator to load the sample to the corresponding reagent strip position;
before the sample adding head adds the sample, the system can also distinguish the sample type through the sample type distinguishing module, and associate the sample type to the sample detection control module, the sample detection control module determines the calculation method (concentration calculation method) according to the sample type;
(4) the sample reaction control module controls the liquid transfer mechanism in the reaction bin to operate so as to complete the uniform mixing and reaction (including cleaning, magnetic attraction and incubation reaction) of the sample and different reagents on the reagent strip;
(5) the reagent strip in the reaction bin is controlled to move to the detection bin through the sample detection control module, and the detection mechanism in the detection bin is used for carrying out luminescence test on the substance to be detected on the reagent strip.
After the detection is finished, the system displays the detection result through the detection result query and display module and stores the detection result on the capacitive touch screen for the user to query.
In addition, after the detection is finished, each module in the system can control the corresponding component to reset, and prompts a user to recover consumables such as the reagent strip and the sample adding head.
In the running process of the system, the system also records the door states of all the reaction bins through the risk avoiding module, and when a certain reaction bin is in a sample adding state, the risk avoiding module presets the door of the reaction bin relatively close to the sample bin into a busy state so as to prompt a user that the door of the reaction bin cannot be opened; if the user forcibly opens the bin door of the reaction bin which is preset to be in a busy state, the risk avoiding module controls the reaction bin in the sample adding state to stop sample adding. Examples are as follows:
a, B, C, D four reaction bins are arranged in the chemiluminescence immunoassay analyzer from left to right, the initial position of the manipulator of the sample adding control module is positioned at the left side of the reaction bin A (the position close to the sample bin); when the reaction bin B is used for sample adding, the manipulator can move to the reaction bin B at any time for sample adding; if the bin door of the A reaction bin is accidentally opened in the sample adding process of the B reaction bin, the conflict of the manipulator and the operation of taking and placing the reagent strip or the Tip head in the A reaction bin can occur, and further damage is caused. Therefore, the system designs a risk avoiding module, and by using the risk avoiding module, the right reaction bin records the bin gate state of the left reaction bin (the reaction bin relatively close to the sample bin), for example, the B reaction bin records the bin gate state of the A reaction bin, and the C reaction bin records the B and the D reaction bin records the C. When the reaction bin B is in a sample adding state, the risk avoiding module presets the bin door of the reaction bin A to be in a busy state, and prompts a user that the bin door of the reaction bin A cannot be opened; if the user forcibly opens the bin door of the reaction bin A, the risk avoiding module can control the reaction bin B to stop sample adding until the bin door of the reaction bin A is closed.
The system of the invention adopts multi-thread control, each reaction bin in the sample adding control module, the sample detection control module and the sample reaction control module respectively corresponds to one task thread, the threads are mutually independent, but synchronous operation is needed when competing for common resources, for example, when a certain reaction bin is used for sample adding or detection, the other reaction bins need to wait.
The system adopts a command distribution mechanism; a communication board is arranged between the system and the circuit board correspondingly controlled by each module, and the communication board is used for receiving the scheduling instruction of the system and returning the corresponding return information to the system in sequence; the communication board does not process complex and time-consuming logic and is only used for distributing and collecting commands, so that the system has higher response speed and higher stability.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (8)
1. A chemiluminescent immunoassay analyzer sample dispatching detection system comprises a sample bin, a consumable bin, a reaction bin and a detection bin, wherein a sample rack is arranged in the sample bin, a sample is placed on the sample rack, a sample adding head is arranged in the consumable bin, and a reagent strip and a liquid transferring mechanism are arranged in the reaction bin; characterized in that, the sample scheduling system comprises:
the sample information acquisition module is used for scanning the identification code of the sample and the position code on the sample rack where the sample is positioned by using the sample code scanner and associating the identification code and the position code with the sample analysis item corresponding to the sample;
the sample information loading module automatically loads a sample analysis item to a certain reagent strip position in a certain reaction bin;
the sample adding control module is used for identifying the reaction bin and the reagent strip position loaded by the sample information loading module, controlling the manipulator to grab an empty sample adding head, enabling the manipulator to move the sample adding head to the corresponding sample analysis item position to extract a sample, and then controlling the manipulator to add the sample to the corresponding reagent strip position;
the sample reaction control module controls the liquid transfer mechanism in the reaction bin to operate so as to complete the uniform mixing and reaction of the sample and the reagent;
and the sample detection control module controls the reagent strip in the reaction bin to move to the detection bin, and the detection mechanism in the detection bin is used for performing luminescence test on the substance to be detected on the reagent strip.
2. The system of claim 1, further comprising a risk evasion module for recording the door status of each reaction chamber, and when a reaction chamber is in a sample loading status, the door of the reaction chamber relatively close to the sample chamber is preset to be busy.
3. The system of claim 1, further comprising a sample type determination module connected to the sample detection control module for determining the type of the sample and associating the type of the sample with the sample detection control module, wherein the sample detection control module determines the calculation method according to the type of the sample.
4. The system for dispatching and detecting the samples of the chemiluminescence immunoassay analyzer of claim 1, further comprising a detection result query and display module connected with the sample detection control module.
5. A dispatching detection method of a chemiluminescent immunoassay analyzer sample dispatching detection system is characterized by comprising the following steps:
(1) acquiring an identification code of a sample and a position code of the sample in a sample bin through a sample information acquisition module, and associating the identification code and the position code with a sample analysis item of the corresponding sample;
(2) automatically loading a sample analysis item to a certain reagent strip position in a certain reaction bin through a sample information loading module;
(3) identifying the reaction bin and the reagent strip position loaded by the sample information loading module through the sample loading control module, controlling the manipulator to grab an empty sample loading head, moving the manipulator to the corresponding sample analysis item position to extract a sample, and then controlling the manipulator to load the sample to the corresponding reagent strip position;
(4) controlling a liquid transfer mechanism in the reaction bin to operate through a sample reaction control module so as to complete the uniform mixing and reaction of the sample and the reagent;
(5) the reagent strip in the reaction bin is controlled to move to the detection bin through the sample detection control module, and the detection mechanism in the detection bin is used for carrying out luminescence test on the substance to be detected on the reagent strip.
6. The method according to claim 5, wherein in step (3), before the sample is injected into the sample injection head, the system first determines the type of the sample through the sample type determination module, and associates the type of the sample with the sample detection control module, and the sample detection control module determines the calculation method according to the type of the sample.
7. The method for detecting the scheduling of the chemiluminescent immunoassay analyzer sample scheduling detection system according to claim 5, wherein after the step (5), the system displays the detection result through the detection result query and display module and stores the detection result on the capacitive touch screen for the user to query.
8. The method of claim 5, wherein the system further records the door status of each reaction chamber through a risk avoiding module, and when a reaction chamber is in a sample loading status, the risk avoiding module pre-sets the door of the reaction chamber relatively close to the sample chamber to a busy status to prompt a user that the door of the reaction chamber cannot be opened; if the user forcibly opens the bin door of the reaction bin which is preset to be in a busy state, the risk avoiding module controls the reaction bin in the sample adding state to stop sample adding.
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