CN113667759B - SNP genetic marker related to egg-laying duration of chicken and application thereof - Google Patents
SNP genetic marker related to egg-laying duration of chicken and application thereof Download PDFInfo
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Abstract
The invention provides SNP genetic markers related to the egg-laying endurance capacity of chickens, which belong to the fields of chicken genetic breeding and biotechnology and comprise POL _ tag9 and/or POL _ tag 17; POL _ tag9 corresponds to position 20037745 of chromosome sense strand No. 9 of sequence information of chicken reference genome Ga l us _ Ga l us-GRCg6 published in NCBI, wherein the base is G or A; POL _ tag17 corresponds to position 2186240 of chromosome sense strand No. 17 of sequence information version No. Ga l us _ Ga l us-GRCg6 of chicken reference genome published in NCBI, wherein bases are G or A, the SNP genetic marker is helpful for genetically improving laying hen later-period egg laying number and can be used for genome selection or molecular marker assisted selection. The invention also provides application of the SNP genetic marker related to the egg laying endurance capacity of the chickens in genetic breeding of the chickens.
Description
Technical Field
The invention belongs to the field of chicken genetic breeding and biotechnology, and particularly relates to an SNP genetic marker related to the egg laying endurance capacity of chickens and application thereof.
Background
The egg yield is a key factor influencing the breeding income of the laying hens, is a main selection character of the breeding of the laying hens, and is also a main research content of the genetic evaluation of the laying hens. With the development of the quantitative genetic theory and the progress of the computing technology, the laying performance of the laying hens is continuously improved, and the peak laying rate approaches the physiological limit of the laying hens. In recent years, the european and american layer breeding company has moved the selection goal from peak laying rates to egg-laying sustainability. The egg-laying duration refers to that the laying hens still maintain a high egg-laying level after reaching the egg-laying peak. The selection strength for improving the egg laying duration is genetically improved, the egg laying number in the later period can be increased, the production efficiency is improved, and the impact on the environment is reduced (Bain et al.
The egg number belongs to the character of function value and can be measured for many times. The random regression model is used for analyzing the current prevalence trend of the function value character, the model describes the target character in a curve form by constructing a genetic covariance equation, and the peak valley of the curve has biological significance and can reflect that the life body accelerates growth or deviates from the original time line. The random regression model is used for analyzing the dynamic change rule of the egg number, the second characteristic value of the genetic effect is selected, the shape of the egg-laying curve can be changed, and the purpose of prolonging the service life of the laying hens is achieved.
In recent years, Genome-wide association assays (GWAS) become a powerful tool for analyzing the quantitative trait genetic structure of livestock and poultry. The GWAS method is used for researching the association between the base variation and the phenotypic value, can identify the genetic marker influencing the economic traits, and is particularly suitable for the traits which are difficult to measure, the late-stage traits of life bodies, the restrictive traits and the slaughtering traits. If the genetic marker for the egg laying continuous ability of the chicken can be screened through whole genome association analysis, the genetic progress can be rapidly acquired through molecular marker assisted breeding or whole genome selection.
Disclosure of Invention
In order to solve the breeding problem of laying hens with strong egg laying persistence, the invention provides the SNP genetic marker related to the egg laying persistence of the laying hens, the SNP genetic marker is beneficial to genetically improving the later-stage egg laying number of the laying hens, and the SNP genetic marker can be used for genome selection or molecular marker-assisted selection.
The invention also provides application of the SNP genetic marker related to the egg-laying duration capability of the chicken in chicken genetic breeding.
The invention is realized by the following technical scheme:
the application provides SNP genetic markers related to the egg-laying endurance of chicken, comprising POL _ tag9 and/or POL _ tag 17;
the SNP number of the POL _ tag9 is rs313804988, which corresponds to the 20037745 th position of the chromosome sense strand 9 of the sequence information of the reference genome Gallus _ Gallus-GRCg6 version of chicken published in NCBI, and belongs to the 3 rd intron of the SEC62 gene, wherein the base is G or A;
the SNP number of the POL _ tag17 is rs315054405, which corresponds to the 2186240 th chromosome sense strand of the sequence information No. 17 of the chicken reference genome Gallus _ Gallus-GRCg6 version published in NCBI, belongs to the downstream of PNPLA7 gene, and the basic group is G or A.
Based on the same inventive concept, the application provides the application of SNP genetic markers related to the egg laying persistence of chickens in genetic breeding of chickens.
Based on the same invention concept, the application provides an early selection method for the egg-laying duration capacity traits, and the early selection is carried out on the egg-laying duration capacity traits based on the genotype of the SNP genetic marker.
Optionally, the method includes:
detecting the genotype of the chicken POL _ tag9 and/or POL _ tag17 to be detected;
early selecting the egg-laying continuous capacity traits of the chicken to be detected based on the genotype of the POL _ tag9 and/or the POL _ tag 17;
wherein the egg production duration of the individual with the GG genotype of the POL _ tag9 is greater than the egg production duration of the individual with the GA genotype, and the egg production duration of the individual with the GA genotype is greater than the egg production duration of the individual with the AA genotype;
the egg-laying duration of the individual with the GG genotype of the POL _ tag17 is greater than that of the individual with the GA genotype, and the egg-laying duration of the individual with the GA genotype is greater than that of the individual with the AA genotype.
Optionally, in the detecting the genotype of the chicken POL _ tag9 and/or POL _ tag17 to be detected, the method for detecting the genotype of the chicken POL _ tag9 to be detected includes:
performing PCR amplification on the genome DNA of the chicken to be detected by taking POL _ P9f and POL _ P9r as primers;
sequencing the PCR amplification product to obtain the 20037745 th genotype of the positive-sense strand of the No. 9 chromosome of the chicken to be detected;
the method for detecting the genotype of the POL _ tag17 of the chicken to be detected comprises the following steps:
performing PCR amplification on the genome DNA of the chicken to be detected by taking POL _ P17f and POL _ P17r as primers;
sequencing the PCR amplification product to obtain the 2186240 th genotype of the positive-sense strand of the 17 th chromosome of the chicken to be detected;
wherein, the base sequence of the POL _ P9f is shown in SEQ ID NO.1, the base sequence of the POL _ P9r is shown in SEQ ID NO.2, the base sequence of the POL _ P17f is shown in SEQ ID NO.3, and the base sequence of the POL _ P17r is shown in SEQ ID NO. 4.
Based on the same inventive concept, the application provides primers for detecting SNP genetic markers related to egg-laying capacity of chickens, comprising a primer for detecting POL _ tag9 and/or a primer for detecting POL _ tag17, wherein the primer for detecting POL _ tag9 comprises POL _ P9f and POL _ P9r, and the primer for detecting POL _ tag17 comprises POL _ P17f and POL _ P17 r;
the base sequence of the POL _ P9f is shown in SEQ ID NO.1, the base sequence of the POL _ P9r is shown in SEQ ID NO.2, the base sequence of the POL _ P17f is shown in SEQ ID NO.3, and the base sequence of the POL _ P17r is shown in SEQ ID NO. 4.
Based on the same inventive concept, the application provides an application of a primer for detecting the SNP genetic marker in chicken genetic breeding.
Based on the same inventive concept, the present application provides a kit comprising a primer for detecting POL _ tag9 and/or a primer for detecting POL _ tag17, wherein the primer for detecting POL _ tag9 comprises POL _ P9f and POL _ P9r, and the primer for detecting POL _ tag17 comprises POL _ P17f and POL _ P17 r.
Based on the same inventive concept, the application provides the application of the kit in chicken genetic breeding.
One or more technical schemes in the invention at least have the following technical effects or advantages:
1. the invention provides SNP genetic markers related to egg laying duration of chickens, the related SNP genetic markers are POL _ tag9 and POL _ tag17, the SNP genetic marker POL _ tag9 can improve the egg laying number of later period by 2.74, the other marker POL _ tag17 can improve the egg laying number of later period by 7.19, any genetic marker or the combination of the genetic markers is favorable for genetically improving the egg laying duration of the chickens and increasing the egg laying number of the later period of the chickens, and the SNP genetic markers can be used for genome selection or molecular marker-assisted selection.
2. The invention provides a primer for detecting an SNP genetic marker related to the egg laying duration of a chicken, the primer is designed according to any one or two of the SNP genetic markers POL _ tag9 and POL _ tag17, is used for amplifying and identifying a genetic locus of a chicken to be detected, has strong specificity and high accuracy, and provides a solid foundation for the breeding of the laying hens.
The foregoing description is only an overview of the technical solutions of the present invention, and the embodiments of the present invention are described below in order to make the technical means of the present invention more clearly understood and to make the above and other objects, features, and advantages of the present invention more clearly understandable.
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In order to more clearly illustrate the technical solutions in the present application, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is an additive egg number genetic characteristic curve constructed based on a random regression model.
FIG. 2 is a Manhattan diagram of genetic markers associated with the egg-laying endurance capacity of chickens.
FIG. 3 is a diagram of genetic markers QQ associated with the egg-laying endurance capacity of chickens.
Detailed Description
The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is a conflict, the present specification will control.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
It should be further noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions.
The SNP genetic markers related to the egg-laying endurance of chickens of the present application will be described in detail below with reference to examples.
Example 1
Random regression analysis yielded pseudophenotypic data:
the experimental chickens come from the resource groups of laying hens of Jiangsu poultry houses, and the information about the group construction is detailed in the literature published by the research team (Guojun et al, application of stochastic regression model to analyze the genetic parameters of egg yolk quality of laying hens [ J]Nanjing university of agriculture, 2016,39(1): 145-149). In short, the Dongxiang green-shell laying hens and the white leghorn chickens are used as parents and are subjected to positive and negative crossing to obtain F1Generation, F2And (4) generation. The experimental chicken is raised in a single cage, mechanically fed and cleared of dung. Egg laying data are counted from 29 weeks to 72 weeks, and the cumulative egg laying number every 4 weeks is recorded as the egg laying number per month.
And preliminarily screening the pedigree records and the production data, removing obvious wrong and repeated data, removing outliers, removing less than 5 record individuals, and sorting into a table form. After data washing, 23494 records remained for the resource population monthly egg production dataset. And determining that the batch and the production day age are listed as fixed effects through one-factor variance analysis. The monthly egg production (covariance) variance component was then analyzed with the WOMBAT software along with genetic parameters. The genetic model formula is as follows:
wherein, yiklIs the number of eggs laid by Kth chickens in the ith week age in the ith batch; HYiIs a batch fixing effect; bmIs the mth fixed regression coefficient; a iskmIs the mth random regression coefficient of additive effect; p is a radical ofkmIs the mth random regression coefficient of the permanent environmental effect; z is a radical ofklmIs an embedded legendre polynomial covariate; e.g. of the typeiklIs the residual effect; n is1、n2、n3Are the embedding fixed effect, additive inheritance and permanent environmental effect legendre polynomial orders.
The invention adopts the AIC criterion and the BIC criterion to select the statistical model most suitable for describing the egg laying number per month and compare the statistical models so as to improve the accuracy of data analysis. Through model comparison and analysis, the random regression model of the monthly egg number preferably embeds 3 th order Legendre polynomial in additive genetic effect, 4 th order Legendre polynomial in permanent environmental effect and 2 nd order Legendre polynomial in fixed regression term. The residual error is treated by heterogeneous treatment and is divided into 8 levels.
And the eigenvalue and eigenvector of the additive genetic matrix are obtained by R calculation. In order to obtain the characteristic equation, calculating lambdae, wherein E is a characteristic vector and lambada is a Legendre polynomial coefficient matrix. The characteristic curve corresponding to the additive genetic effect of the number of eggs laid by the laying hen resource group in each month is shown in figure 1, the additive genetic 1 st characteristic curve is nearly linear, and the whole course is positive. The 2 nd characteristic curve shows a descending trend with increasing week age, and is negative after 55 weeks of age. The 3 rd characteristic curve shows cosine wave trend, the age of 42-64 weeks is a positive value, and the rest are negative values. According to the characteristic equation, the curve of the laying hens can be changed by selecting the second characteristic equation coefficient of the additive genetic coefficient matrix, and the machine is suitable for being 32-55 weeks old during selection. The second characteristic equation coefficient of the egg number additive genetic coefficient matrix is an index for judging egg laying duration, and the egg laying number of laying hens in the later period can be increased by selecting the second characteristic equation coefficient. Thus, the present invention lists the second characteristic value as pseudophenotypic data for GWAS analysis.
Example 2
Whole genome association analysis of egg-laying sustainability
A disposable syringe is used for collecting about 2ml of blood sample from the vein of a test chicken wing, and the blood sample is placed into a BD anticoagulation tube (Bydi medical equipment Co., Ltd. Suzhou) and stored at 70 ℃. Extracting genome DNA from blood sample, detecting by 0.8% agarose electrophoresis and ultraviolet spectrophotometry, diluting DNA sample to 50 + -5 ng/μ l after qualified, and using for gene chip typing.
Chicken high-density gene chip using Onfei corporation
The 600K Chicken Genotyping Array carries out Genotyping, and the quality control of data is carried out by referring to a chip specification, and the method mainly comprises the following steps: performing quality control before parting by using APT software; quality control is carried out by PLINK, and SNP markers with detection rate lower than 0.97 and deviating from Hardy Weinberg balance are removed; analyzing information of metrics.R, SNP _ filter.R, SNP CR and FLD, and screening SNP; fill in genotype with BEAGLE. Left after quality controlThe remaining 435867 SNPs and 1512 samples were used for subsequent analysis.
Before the whole genome association analysis, multi-dimensional principal component analysis is carried out to eliminate false positive, and the former 5 principal components are used as covariate parameters to be added into the fixed effect of the genetic model. The independent test estimation of each SNPs locus is calculated by using an R script "simpleM" method, and 59308 independent markers are obtained. Correction was performed using Bonferroni to obtain a significant genome threshold of 8.43 × 10-7The genome proposal threshold is 1.69X 10-5. The egg laying duration pseudophenotype values obtained in example 1 were analyzed by a mixed linear model to obtain individual SNPs signature significance test P values. The matrix expression of the linear model is as follows,
y＝Wα+xβ+u+ε
wherein y represents a sample phenotype value vector; w represents a covariance matrix; alpha is an intercept vector; x-tagged genotype vector; u is a random effect vector; ε is the residual error.
The whole genome association analysis was performed on the second characteristic coefficient of additive genetic effect of the egg production number of 1512 chickens, and the results are shown in fig. 2 and fig. 3. According to the Manhattan graph, significant genome level markers exist in the chromosomes 9 and 17 of the chicken, and genome suggestive markers exist in the chromosomes 1 and 8 of the chicken. The QQ diagram further verifies that the GWAS result is reliable. Summarizing the screened egg-laying duration genetic markers:
TABLE 1 genomic significance level of genetic markers
Note that: the marker chromosomal physical location is referenced to the chicken whole genome (Gallus galllus GRCg 6).
Example 3
Verification of genetic markers
The 2 SNP genetic markers are applied to carry out association analysis on resource groups of Dongxiang green-shell layer chickens-white leghorned chickens. The specific operation steps are as follows:
1) PCR primers: DNA template sequence information was downloaded from the NCBI website and PCR amplification primers were designed using primer premier software, with primer information as shown in Table 2. PCR primers were synthesized by Biotechnology engineering (Shanghai) Ltd.
TABLE 2 amplification primers for genetic markers for detection of egg-laying sustainability
2) Extracting genome DNA: 1482 parts of blood sample genome DNA is extracted by a CTAB method, and PCR amplification is carried out after qualified detection by an ultraviolet spectrophotometer and agarose electrophoresis.
3) And (3) PCR amplification process:
reaction system: the 10. mu.l system included 50ng of the identification material DNA template, 0ng of each of the forward and reverse primers, 5. mu.L of 2 XPower Taq MasterMix, and the remaining volume was made up with ultrapure water.
Reaction procedure: firstly, denaturation at 94 ℃ for 30s, annealing at 51-54 ℃ for 30s, and extension at 72 ℃ for 30s for 5 cycles; then denaturation at 94 ℃ for 30s, annealing at 51-54 ℃ for 30s, and extension at 72 ℃ for 30s for 30 cycles; extending for 5min at 72 ℃, and storing at 4 ℃.
4) The amplified product is sent to a sequencing company for sequence polymorphism detection.
5) Correlation analysis: the individuals tested all had genotypes and eggs laid at 57-72 weeks of age, and then were subjected to correlation analysis. The results of the analysis are shown in Table 3, and the dominant genotypes of both the genetic markers Tag9 and Tag17 are AA.
TABLE 3 correlation of genetic markers with later egg production
2 SNP genetic markers related to the egg-laying continuous ability are obtained through screening by random regression analysis and whole genome association analysis. Verification experiments show that the selection of the favorable genotype of 2 SNP genetic markers can improve the later-period egg laying number of the laying hens.
The primer combination can be used for breeding to improve the egg laying endurance capacity, and can also be used for preparing a kit.
One or more technical solutions in the present application at least have the following technical effects or advantages:
(1) the SNP genetic markers related to the egg laying capacity of the laying hens are POL _ tag9 and POL _ tag17, the POL _ tag9 can increase the egg laying number of the laying hens at the later period by 2.74, the POL _ tag17 can increase the egg laying number of the laying hens at the later period by 7.19, and the genetic marker or the combination of the genetic markers is favorable for genetically improving the egg laying capacity of the laying hens and increasing the egg laying number of the laying hens at the later period, and can be used for genome selection or molecular marker-assisted selection.
(2) The primer for detecting the SNP genetic marker related to the egg laying duration of the laying hens is designed according to any one or two of the SNP genetic markers POL _ tag9 and POL _ tag17, is used for amplifying and identifying the genetic locus of the laying hens to be detected, has strong specificity and high accuracy, and provides a solid foundation for the breeding of the laying hens.
Finally, it should be further noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
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Xueyi science and technology (Chengdu) Co Ltd
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Claims (4)
1. The application of the primer for detecting the SNP genetic marker related to the egg-laying duration of the chicken in chicken genetic breeding is characterized in that the chicken genetic breeding is the genetic breeding of 57-72-week-old chicken about egg-laying duration traits, and the variety of the chicken is Dongxiang green-shell laying chicken and/or white leghorns;
the SNP genetic markers related to the egg-laying endurance capacity of the chicken comprise POL _ tag9 and/or POL _ tag 17;
the SNP number of the POL _ tag9 is rs313804988, which corresponds to the 20037745 th chromosome sense strand of the sequence information No. 9 chromosome of a chicken reference genome Gallus _ Gallus-GRCg6 published in NCBI, wherein the base is G or A; the SNP number of the POL _ tag17 is rs315054405, which corresponds to the 2186240 th chromosome sense strand of sequence information No. 17 of the chicken reference genome Gallus _ Gallus-GRCg6 version published in NCBI, and the base is G or A;
wherein the egg production duration of the individual with the GG genotype of the POL _ tag9 is greater than the egg production duration of the individual with the GA genotype, and the egg production duration of the individual with the GA genotype is greater than the egg production duration of the individual with the AA genotype;
the egg-laying duration of the individual with the GG genotype of the POL _ tag17 is greater than that of the individual with the GA genotype, and the egg-laying duration of the individual with the GA genotype is greater than that of the individual with the AA genotype;
the primers for detecting the SNP genetic markers related to the egg laying capacity of the chickens comprise primers for detecting POL _ tag9 and/or POL _ tag17, the primers for detecting POL _ tag9 comprise POL _ P9f and POL _ P9r, and the primers for detecting POL _ tag17 comprise POL _ P17f and POL _ P17 r;
the base sequence of the POL _ P9f is shown in SEQ ID NO.1, the base sequence of the POL _ P9r is shown in SEQ ID NO.2, the base sequence of the POL _ P17f is shown in SEQ ID NO.3, and the base sequence of the POL _ P17r is shown in SEQ ID NO. 4.
2. The application of the kit in chicken genetic breeding is characterized in that the chicken genetic breeding is genetic breeding of 57-72-week-old chicken about egg-laying continuous ability traits, and the variety of the chicken is Dongxiang green-shell laying chicken and/or white leghorn chicken;
the kit comprises primers for detecting SNP genetic markers related to the egg-laying continuous capability of chickens, wherein the primers for detecting the SNP genetic markers related to the egg-laying continuous capability of chickens comprise primers for detecting POL _ tag9 and/or primers for detecting POL _ tag17, the primers for detecting POL _ tag9 comprise POL _ P9f and POL _ P9r, and the primers for detecting POL _ tag17 comprise POL _ P17f and POL _ P17 r;
the base sequence of the POL _ P9f is shown in SEQ ID NO.1, the base sequence of the POL _ P9r is shown in SEQ ID NO.2, the base sequence of the POL _ P17f is shown in SEQ ID NO.3, and the base sequence of the POL _ P17r is shown in SEQ ID NO. 4;
the SNP genetic markers related to the egg-laying endurance capacity of the chicken comprise POL _ tag9 and/or POL _ tag 17;
the SNP number of the POL _ tag9 is rs313804988, which corresponds to the 20037745 th position of the chromosome 9 sense strand of the sequence information of the chicken reference genome Gallus _ Gallus-GRCg6 published in NCBI, wherein the base is G or A; the SNP number of the POL _ tag17 is rs315054405, which corresponds to the 2186240 th chromosome sense strand of sequence information No. 17 of the chicken reference genome Gallus _ Gallus-GRCg6 published in NCBI, and the base is G or A;
wherein the egg production duration of the individual with the GG genotype of the POL _ tag9 is greater than the egg production duration of the individual with the GA genotype, and the egg production duration of the individual with the GA genotype is greater than the egg production duration of the individual with the AA genotype;
the egg-laying duration of the individual with the GG genotype of the POL _ tag17 is greater than that of the individual with the GA genotype, and the egg-laying duration of the individual with the GA genotype is greater than that of the individual with the AA genotype.
3. A method for early selection of a chicken egg-laying sustainability trait, the method comprising:
detecting the genotype of the chicken POL _ tag9 and/or POL _ tag17 to be detected;
early selecting the egg-laying continuous capacity traits of the chicken to be tested at the age of 57-72 weeks based on the genotype of the POL _ tag9 and/or the POL _ tag 17;
wherein the egg production duration of the individual with the GG genotype of the POL _ tag9 is greater than the egg production duration of the individual with the GA genotype, and the egg production duration of the individual with the GA genotype is greater than the egg production duration of the individual with the AA genotype;
the egg-laying duration of the individual with the GG genotype of the POL _ tag17 is greater than that of the individual with the GA genotype, and the egg-laying duration of the individual with the GA genotype is greater than that of the individual with the AA genotype;
the variety of the chicken to be detected is Dongxiang green-shell laying hens and/or white leghorns;
the SNP number of the POL _ tag9 is rs313804988, which corresponds to the 20037745 th position of the chromosome 9 sense strand of the sequence information of the chicken reference genome Gallus _ Gallus-GRCg6 published in NCBI, wherein the base is G or A; the SNP number of the POL _ tag17 is rs315054405, which corresponds to the 2186240 th chromosome of the 17 th chromosome sense strand of the sequence information of the chicken reference genome Gallus _ Gallus-GRCg6 published in NCBI, and the base is G or A.
4. The method for early selection of chicken egg-laying duration capability trait according to claim 3, wherein the method for detecting the genotype of POL _ tag9 in the genotypes of the chicken to be detected POL _ tag9 and/or POL _ tag17 comprises the following steps:
performing PCR amplification on the genome DNA of the chicken to be detected by taking POL _ P9f and POL _ P9r as primers;
sequencing the PCR amplification product to obtain the 20037745 th genotype of the positive-sense strand of the No. 9 chromosome of the chicken to be detected;
the method for detecting the genotype of the POL _ tag17 of the chicken to be detected comprises the following steps:
performing PCR amplification on the genome DNA of the chicken to be detected by taking POL _ P17f and POL _ P17r as primers;
sequencing the PCR amplification product to obtain the 2186240 th genotype of the positive-sense strand of the 17 th chromosome of the chicken to be detected;
wherein, the base sequence of the POL _ P9f is shown in SEQ ID NO.1, the base sequence of the POL _ P9r is shown in SEQ ID NO.2, the base sequence of the POL _ P17f is shown in SEQ ID NO.3, and the base sequence of the POL _ P17r is shown in SEQ ID NO. 4.
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| CN107354220A (en) * | 2017-08-24 | 2017-11-17 | 中国农业大学 | A kind of molecular labeling related to chicken egg number character and its application |
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