CN113663119A - Preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function - Google Patents
Preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function Download PDFInfo
- Publication number
- CN113663119A CN113663119A CN202110765302.8A CN202110765302A CN113663119A CN 113663119 A CN113663119 A CN 113663119A CN 202110765302 A CN202110765302 A CN 202110765302A CN 113663119 A CN113663119 A CN 113663119A
- Authority
- CN
- China
- Prior art keywords
- silk fibroin
- iodized oil
- silk
- microsphere
- preparing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108010022355 Fibroins Proteins 0.000 title claims abstract description 58
- 239000004005 microsphere Substances 0.000 title claims abstract description 38
- 208000005189 Embolism Diseases 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 31
- 230000010102 embolization Effects 0.000 claims abstract description 25
- 238000005119 centrifugation Methods 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 239000000725 suspension Substances 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 8
- 239000000835 fiber Substances 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 7
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims abstract description 4
- 238000000227 grinding Methods 0.000 claims abstract description 4
- 239000012188 paraffin wax Substances 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000000502 dialysis Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 241001411320 Eriogonum inflatum Species 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000003208 petroleum Substances 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 238000000935 solvent evaporation Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 238000012800 visualization Methods 0.000 claims 6
- 238000006731 degradation reaction Methods 0.000 abstract description 7
- 230000015556 catabolic process Effects 0.000 abstract description 6
- 238000001727 in vivo Methods 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000005457 optimization Methods 0.000 description 7
- 230000009286 beneficial effect Effects 0.000 description 5
- 201000009274 endometriosis of uterus Diseases 0.000 description 5
- 208000005641 Adenomyosis Diseases 0.000 description 4
- 206010061692 Benign muscle neoplasm Diseases 0.000 description 4
- 201000004458 Myoma Diseases 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 210000000685 uterine artery Anatomy 0.000 description 4
- 230000000302 ischemic effect Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000017074 necrotic cell death Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000002594 fluoroscopy Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 206010046798 Uterine leiomyoma Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/108—Specific proteins or polypeptides not covered by groups A61L24/102 - A61L24/106
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/02—Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function, which comprises the steps of preparing degummed silk fibroin fiber; preparing silk fibroin solution and concentrating; adding paraffin and Span80 into a four-neck flask, stirring uniformly, and dropwise adding concentrated silk fibroin solution and super liquid iodized oil to obtain suspension; adding the suspension into a centrifuge tube for centrifugation, taking out the centrifugate, cleaning, and centrifuging after cleaning; adding ethanol overnight, removing ethanol, drying, and grinding into uniform powder to obtain iodized oil-fibroin protein embolism microsphere. The iodized oil is added, so that the silk fibroin embolization microsphere has an X-ray perspective developing function, the degradation period of the iodized oil silk fibroin microsphere is 1 month to half a year, and the problem of permanent detention in vivo is solved.
Description
Technical Field
The invention relates to the technical field of medical embolism microspheres, in particular to a preparation method of iodized oil silk fibroin embolism microspheres with perspective developing function.
Background
The percutaneous puncture uterine artery embolism is characterized in that embolism microspheres are conveyed to a uterine artery through a microcatheter, and hysteromyoma and adenomyosis are treated by blocking blood supply.
At present, the most clinically used embolizing microspheres are PVA particles, which are permanent embolization agents, and the particles cannot be degraded in vivo and are permanently retained in vivo, so that trouble and worry are often brought to patients; meanwhile, permanent embolization is not beneficial to secondary treatment of uterine artery embolization.
The silk fibroin microsphere has a degradable function, can be used for treating myoma adenomyosis of uterus by uterine artery embolization, but the traditional silk fibroin microsphere is not developable by X-ray fluoroscopy, is not beneficial to evaluating the embolization degree in the embolization process, and is not beneficial to detecting the degradation condition of the silk fibroin microsphere in vitro. Therefore, a degradable silk fibroin embolization microsphere is needed, which can not only ensure the ischemic necrosis of myoma and adenomyosis, but also solve the problem of permanent retention in vivo, and has the X-ray fluoroscopy and development function, thereby being beneficial to the in vitro monitoring of the degradation process.
Disclosure of Invention
The invention aims to provide a preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the technical scheme that: the preparation method of the iodized oil silk fibroin embolism microsphere with perspective developing function comprises the following steps,
s1) preparing degummed silk fibroin fibers;
s2) preparing silk fibroin solution and concentrating;
s3) preparing the fibroin microsphere by an emulsion solvent evaporation method, which comprises the following steps,
s31) adding paraffin and Span80 into a four-neck flask, uniformly stirring, dropwise adding the concentrated silk fibroin solution and super iodized oil prepared in the S2, plugging a bottle stopper, opening the bottle stopper, and continuously stirring to obtain a suspension;
s32) adding the suspension into a centrifugal tube for centrifugation, taking out the centrifugate, adding absolute ethyl alcohol, washing with petroleum ether and absolute ethyl alcohol, and centrifuging after washing;
s33), adding ethanol overnight, removing the ethanol, drying, grinding the dried product into uniform powder, and obtaining the iodized oil silk fibroin embolization microsphere.
As a further optimization, the specific step of S1 is to put raw silk into sodium carbonate solution to make the silk uniformly spread, take out the silk after 30min, rub the silk with warm water, rub the silk with deionized water, and then put the silk into a constant temperature oven to dry, thus obtaining the degummed fibroin fiber.
As a further optimization, the specific steps of S2 are,
s21) putting the degummed silk fibroin fiber into LiBr solution, magnetically stirring in a constant-temperature oil bath to obtain SF-LiBr mixed solution, putting the mixed solution into a dialysis bag, fastening two ends, dialyzing in deionized water, and filtering to obtain silk fibroin solution;
s22) placing the dialysis bag into polyethylene glycol powder for concentration, centrifuging the concentrated silk fibroin solution, and collecting the supernatant for later use.
As a further optimization, the dialysis time in S21 is 3-5 days, and water is changed every 8-12 h.
As a further optimization, the dialysis time in S22 is 10-15 h; the centrifugation temperature is 3-5 ℃, the centrifugation radius is 13-15cm, the centrifugation time is 40-60min, and the centrifugation speed is 4500-.
As a further optimization, the stirring speed in S31 is 900-; the sealing time of the bottle stopper is 3-5 min; the stirring time after the bottle stopper is opened is 40-60 min.
As a further optimization, the centrifugation temperature of the suspension in S32 is 3-5 ℃, the centrifugation time is 5-10min, and the centrifugation speed is 1300-; the number of washing in S32 was 3 to 5.
As a further optimization, the drying temperature in S33 is 20 + -2 deg.C.
Compared with the prior art, the invention has the beneficial effects that:
by mixing iodized oil in a certain proportion in silk fibroin, the silk fibroin embolization microsphere has an X-ray perspective developing function on the premise of not changing the degradability of the silk fibroin, overcomes the defects of the traditional silk fibroin microsphere, and is favorable for evaluating the embolization degree in the operation and monitoring the degradation condition in vitro; meanwhile, the degradation period is 1 month to half a year, so that the ischemic necrosis of myoma and adenomyosis can be guaranteed, and the problem of permanent detention in vivo can be solved.
Drawings
FIG. 1 is an X-ray perspective view of the empty dish of the present invention.
Fig. 2 is a schematic diagram of iodized oil-filled silk fibroin embolization microspheres of the present invention.
Fig. 3 is a perspective X-ray development of the iodized oil silk fibroin embolization microsphere.
Fig. 4 is an electron microscope image of the iodized oil-filled silk fibroin embolization microsphere of the present invention.
Detailed Description
The following are specific embodiments of the present invention, and the technical solutions of the present invention will be further described with reference to the drawings, but the present invention is not limited to these embodiments.
As shown in fig. 1 to 4, the preparation method of iodized oil silk fibroin embolization microspheres with perspective developing function comprises the following steps of weighing 5g of raw silk, putting the raw silk into 2L of sodium carbonate solution with 100 ℃ and 0.02mol/L, overturning the raw silk by using a glass rod every 5min to uniformly distribute the silk, taking out the raw silk after 30min, rubbing and washing the raw silk for 3 times by using warm water, rubbing and washing the raw silk for 3 times by using deionized water to fully degum, and then putting the raw silk into a constant-temperature oven for drying to obtain degummed silk fibroin fibers; putting the silk fibroin fiber into 9.3mol/L LiBr solution (200g/L), and magnetically stirring for 4 hours in a constant-temperature oil bath at 60 ℃ to obtain brown-yellow SF-LiBr mixed solution; placing the mixed solution into a dialysis bag, fastening two ends, dialyzing in deionized water for 3 days, periodically changing water, and filtering to obtain silk fibroin solution; and then placing the dialysis bag into polyethylene glycol powder for concentrating for 12h, centrifuging the concentrated fibroin solution at 4 deg.C with a centrifugal radius of 13.5cm and a rotation speed of 4950r/min for 1h, and storing the supernatant in a refrigerator at 4 deg.C for use.
Preparing fibroin microspheres by adopting an emulsion solvent evaporation method, respectively adding 20mL of paraffin and 200 mu L of Span80 into a four-mouth flask, uniformly stirring by using a mechanical stirrer at the rotating speed of 950r/min, dropwise adding a fibroin protein solution with the volume fraction of 8% and 400 mu L of super-liquid iodized oil, plugging a wool glass plug for 5min, opening the plug, continuously stirring for 1h, adding the obtained suspension into a 50mL centrifuge tube, centrifuging at 4 ℃ and 1350r/min for 5min, taking out, dropwise adding 2mL of anhydrous ethanol, washing for 3 times by using petroleum ether and anhydrous ethanol, immediately centrifuging after washing for 1 time, finally adding ethanol overnight, pouring out the ethanol, volatilizing the residual ethanol, drying at room temperature, grinding the product into uniform powder, and obtaining the iodized oil fibroin microspheres.
The degradation period of the iodized oil silk fibroin microspheres is 1 month to half a year, so that the ischemic necrosis of myoma and adenomyosis can be ensured, and the problem of permanent detention in vivo can be solved; because iodized oil is added, the silk fibroin embolization microsphere has the X-ray perspective developing function, and is favorable for intraoperative evaluation of embolization degree and in-vitro monitoring of degradation condition.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.
Claims (8)
1. The preparation method of the iodized oil silk fibroin embolism microsphere with perspective developing function is characterized by comprising the following steps,
s1) preparing degummed silk fibroin fibers;
s2) preparing silk fibroin solution and concentrating;
s3) preparing the fibroin microsphere by an emulsion solvent evaporation method, which comprises the following steps,
s31) adding paraffin and Span80 into a four-neck flask, uniformly stirring, dropwise adding the concentrated silk fibroin solution and super iodized oil prepared in the S2, plugging a bottle stopper, opening the bottle stopper, and continuously stirring to obtain a suspension;
s32) adding the suspension into a centrifugal tube for centrifugation, taking out the centrifugate, adding absolute ethyl alcohol, washing with petroleum ether and absolute ethyl alcohol, and centrifuging after washing;
s33), adding ethanol overnight, removing the ethanol, drying, grinding the dried product into uniform powder, and obtaining the iodized oil silk fibroin embolization microsphere.
2. The method for preparing iodized oil silk fibroin embolization microspheres with perspective developable function according to claim 1, wherein the step S1 comprises the steps of putting raw silk into sodium carbonate solution to uniformly spread the silk, taking out the silk after 30min, rubbing the silk with warm water, rubbing the silk with deionized water, and drying the silk in a constant-temperature oven to obtain the degummed silk fibroin.
3. The method for preparing iodized oil silk fibroin embolization microspheres with perspective visualization function according to claim 1, wherein S2 comprises the following steps,
s21) putting the degummed silk fibroin fiber into LiBr solution, magnetically stirring in a constant-temperature oil bath to obtain SF-LiBr mixed solution, putting the mixed solution into a dialysis bag, fastening two ends, dialyzing in deionized water, and filtering to obtain silk fibroin solution;
s22) placing the dialysis bag into polyethylene glycol powder for concentration, centrifuging the concentrated silk fibroin solution, and collecting the supernatant for later use.
4. The method for preparing iodized oil silk fibroin embolization microspheres with perspective visualization function of claim 1, wherein the dialysis time in S21 is 3-5 days, and water is changed every 8-12 h.
5. The preparation method of the iodized oil silk fibroin embolization microsphere with perspective visualization function according to claim 1, wherein the dialysis time in S22 is 10-15 h; the centrifugation temperature is 3-5 ℃, the centrifugation radius is 13-15cm, the centrifugation time is 40-60min, and the centrifugation speed is 4500-.
6. The method for preparing iodized oil silk fibroin embolization microspheres with perspective visualization function according to claim 1, wherein the stirring speed in S31 is 900-1000 r/min; the sealing time of the bottle stopper is 3-5 min; the stirring time after the bottle stopper is opened is 40-60 min.
7. The preparation method of the iodized oil silk fibroin embolization microsphere with the perspective visualization function as claimed in claim 1, wherein the centrifugation temperature of the suspension in S32 is 3-5 ℃, the centrifugation time is 5-10min, and the centrifugation speed is 1300-1400 r/min; the number of washing in S32 was 3 to 5.
8. The method for preparing iodized oil silk fibroin embolization microspheres with perspective visualization function of claim 1, wherein the drying temperature in S33 is 20 ± 2 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110765302.8A CN113663119A (en) | 2021-07-07 | 2021-07-07 | Preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110765302.8A CN113663119A (en) | 2021-07-07 | 2021-07-07 | Preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113663119A true CN113663119A (en) | 2021-11-19 |
Family
ID=78538617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110765302.8A Pending CN113663119A (en) | 2021-07-07 | 2021-07-07 | Preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113663119A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101244277A (en) * | 2008-02-14 | 2008-08-20 | 苏州大学 | Medicine carrying fibroin microsphere and preparation thereof |
| CN101652151A (en) * | 2007-01-12 | 2010-02-17 | 李艳芳 | Biodegradable imaging microspheres vascular embolization material |
| CN103965310A (en) * | 2014-04-21 | 2014-08-06 | 浙江大学 | Self-assembling method for preparing fibroin microspheres |
| CN104592375A (en) * | 2014-12-16 | 2015-05-06 | 苏州丝美特生物技术有限公司 | Method for preparing fibroin microspheres by using polyethylene glycol |
| CN105999434A (en) * | 2016-05-24 | 2016-10-12 | 德州海利安生物科技股份有限公司 | Developing type degradable ureter repairing stent |
-
2021
- 2021-07-07 CN CN202110765302.8A patent/CN113663119A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101652151A (en) * | 2007-01-12 | 2010-02-17 | 李艳芳 | Biodegradable imaging microspheres vascular embolization material |
| CN101244277A (en) * | 2008-02-14 | 2008-08-20 | 苏州大学 | Medicine carrying fibroin microsphere and preparation thereof |
| CN103965310A (en) * | 2014-04-21 | 2014-08-06 | 浙江大学 | Self-assembling method for preparing fibroin microspheres |
| CN104592375A (en) * | 2014-12-16 | 2015-05-06 | 苏州丝美特生物技术有限公司 | Method for preparing fibroin microspheres by using polyethylene glycol |
| CN105999434A (en) * | 2016-05-24 | 2016-10-12 | 德州海利安生物科技股份有限公司 | Developing type degradable ureter repairing stent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106479195B (en) | A kind of nano-cellulose enhancing fibroin albumen composite material and preparation method thereof | |
| CN111748109B (en) | Chitosan microspheres prepared from chitosan solution with pH value of 6-8 and preparation method thereof | |
| CN104264369A (en) | Two-step crosslinking preparation method for keratin/PEO biological nanofiber membrane | |
| CN105249576B (en) | A kind of manufacture method of multifunctional clothes package material | |
| CN111840439B (en) | Method for preparing traditional Chinese medicine medicated thread for treating high-level complex anal fistula | |
| CN103083720B (en) | Silk fibroin tube and preparation method thereof | |
| CN107397973B (en) | Four-layer coaxial fiber wound dressing and preparation method thereof | |
| CN105297289B (en) | A kind of fibroin albumen energy-saving and temperature-regulating fiber membrane preparation method | |
| US20210260241A1 (en) | Chitin/graphene composite sponge and preparation method and use thereof | |
| CN108310461B (en) | Porous silk fibroin spinal cord stent with directional release function, and preparation method, preparation mold and application thereof | |
| CN105521522A (en) | Preparation method of silk fibroin and chitosan three-dimensional scaffold material | |
| CN113663119A (en) | Preparation method of iodized oil silk fibroin embolism microsphere with perspective developing function | |
| CN107130333B (en) | A kind of alginic acid and chitosan shuffling fiber and preparation method thereof | |
| CN107137748A (en) | A kind of nucleocapsid electrostatic spinning chitosan nano fiber wound dressing and preparation method thereof | |
| CN110721346A (en) | Biological 3D printing ink and preparation method thereof | |
| CN115624655B (en) | Chitosan-oxidized sodium alginate hydrogel material and preparation method and application thereof | |
| CN106009002A (en) | Chitosan/montmorillonite nano composite hydrogel and preparation method thereof | |
| CN103436992B (en) | Method for preparing nano drug-carrying capsule-loaded alginate fibers | |
| CN105362238B (en) | A kind of Enoxaparin ejection preparation and its industrialized preparing process | |
| CN114441523A (en) | Preparation method of kit for detecting sulfhydryl metabolites in urine | |
| CN111840630B (en) | Bacteriostatic absorbable medical soft tissue suture line and preparation method and application thereof | |
| GB2220881A (en) | Improvements in or relating to superabsorbent materials | |
| CN111097065B (en) | Carbon fiber-based porous material, preparation method and application thereof | |
| CN106729981B (en) | A kind of silk fibroin nano-fiber and its preparation method and application | |
| CN108997619A (en) | A kind of mesoporous silicon oxide of carbonate and buffer solution duplicate protection and cellulose blending film and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211119 |
|
| RJ01 | Rejection of invention patent application after publication |