CN113652349A - Development of in-vitro aptamer analysis method and preliminary pharmacokinetic experimental equipment - Google Patents

Development of in-vitro aptamer analysis method and preliminary pharmacokinetic experimental equipment Download PDF

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CN113652349A
CN113652349A CN202110976689.1A CN202110976689A CN113652349A CN 113652349 A CN113652349 A CN 113652349A CN 202110976689 A CN202110976689 A CN 202110976689A CN 113652349 A CN113652349 A CN 113652349A
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shell
pipe body
plate
water pump
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CN113652349B (en
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张永深
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Vanworld Pharmaceutical Rugao Co Ltd
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Vanworld Pharmaceutical Rugao Co Ltd
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Abstract

The invention discloses a device for developing a vertical aptamer internal analysis method and performing a primary pharmacokinetic experiment, which comprises a first shell, a support rod, a support plate, a main body component, a vibration component and a detection component, wherein the first shell is provided with a first opening, a second opening and a third opening, and the third opening is provided with a second opening: a main body assembly is arranged in the first shell and comprises a water pump, a first pipe body, a second shell and a third pipe body; according to the invention, the third tube body is adopted to simulate blood vessels of a human body, the water pump simulates the heart, the second shell body simulates various large organs, the vaccinia virus inflammatory rabbit skin extract injection is dispersed in water, the model generator generates model information, and sends a signal to the controller to be displayed on the display screen, so that the moving track of the vaccinia virus inflammatory rabbit skin extract injection in the human body and the reaction with pathological change tissues are simulated, the analysis in the aptamer is carried out, the preliminary pharmacokinetics is realized, the reaction in the human body is analyzed, expensive instruments or experimental bodies such as mice and the like are not needed for carrying out experiments, and the cost is low.

Description

Development of in-vitro aptamer analysis method and preliminary pharmacokinetic experimental equipment
Technical Field
The invention relates to the technical field of pharmacokinetic experimental equipment, in particular to equipment for developing an immediately-readapted intra-analysis method and primarily pharmacokinetic experimental equipment.
Background
The injection is prepared from extract of rabbit fur causing inflammation caused by vaccinia virus, and is used for treating neck, shoulder and wrist syndromes; relieving pain, cold feeling, numbness and other symptoms of patients with lumbago; symptomatic neuralgia.
Pharmacokinetics is a subject for quantitatively researching absorption, distribution, metabolism and excretion rules of drugs in organisms and explaining the rule of blood concentration changing along with time by applying mathematical principles and methods.
Any drug requires extensive experimentation before it is put to use, and prior art experimental facilities for the development of in vitro aptamer assays and the use of preliminary pharmacokinetics have not been developed further.
Therefore, a development method of the immediate aptamer internal analysis and a preliminary pharmacokinetic experiment device are provided.
Disclosure of Invention
The invention aims to provide a development method of a real-time aptamer internal analysis method and a preliminary pharmacokinetic experiment device, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a development and preliminary pharmacokinetic experiment equipment of an in-vitro aptamer analysis method comprises a first shell, a support rod, a support plate, a main body component, a vibration component and a detection component, wherein the support rod is arranged on the first shell, the support plate is arranged on the support rod, the main body component is arranged on the support plate, and the vibration component is arranged on the detection component: a main body assembly is arranged in the first shell and comprises a water pump, a first pipe body, a second shell and a third pipe body;
four second shells are symmetrically welded in the first shell, a water outlet of the water pump is communicated with one end of the first pipe body, the other end of the first pipe body is communicated with one side of one second shell, a water inlet of the water pump is communicated with one end of one second pipe body, and the other end of the second pipe body is communicated with one side of the other second shell; the other two second shells and the first two second shells are communicated with each other through a third pipe body;
the vibration assembly is mounted on the upper surface of the support plate and the upper surface of the second shell;
the detection assembly is installed on one side of the water pump and the outer side of the second shell.
Preferably: four supporting rods are symmetrically welded on the upper surface of the first shell, and a supporting plate is welded at the tops of the supporting rods.
Preferably: the vibration assembly comprises a vibration motor, a first transmission plate, a transmission rod, a second transmission plate, a fourth pipe body, a fifth pipe body and a valve;
the upper surface welding of backup pad has first transmission board, the upper surface mid-mounting of first transmission board has vibrating motor.
Preferably: the embedded welding of the front surface of backup pad has four transfer lines, the welding of the bottom of transfer line has the second driving plate, the lower surface of second driving plate welds in the upper surface middle part of second casing.
Preferably: the upper surface of the second shell is communicated with a fourth pipe body, and one side of the second shell is communicated with a fifth pipe body.
Preferably: and valves are arranged on the outer sides of the fourth pipe body and the fifth pipe body.
Preferably: the detection assembly comprises a viscosity detector, a temperature sensor, a display screen, a controller, a model generator, an electric heating plate and a partition plate;
the viscosity detector is installed on the upper surface of a second shell, the controller is installed on one side of the water pump, and the signal output end of the viscosity detector is in signal connection with the signal input end of the controller.
Preferably: the electric heating plate is installed on the inner bottom wall of the second shell, the temperature sensor is installed on one side of the second shell, a probe of the temperature sensor penetrates through the inside of the second shell, and the signal output end of the temperature sensor is in signal connection with the signal input end of the controller.
Preferably: the inside wall welding of second casing has the baffle, the baffle is located the top of electric heating board.
Preferably: a display screen and a model generator are installed on one side of the water pump, a signal end of the model generator is in signal connection with a signal end of the controller, and a signal input end of the display screen is in signal connection with a signal output end of the controller;
the model generator is configured to generate model information.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the third tube body is adopted to simulate the blood vessel of a human body, the water pump simulates the heart, the second shell body simulates various large organs, the vaccinia virus inflammatory rabbit skin extract injection is dispersed in the water body, the model generator generates model information and sends a signal to the controller to be displayed on the display screen, the moving track of the vaccinia virus inflammatory rabbit skin extract injection in the human body and the reaction with the pathological change tissue are simulated, the analysis in the aptamer is carried out, the preliminary pharmacokinetics is realized, the reaction in the human body is analyzed, expensive instruments or experimental bodies such as mice and the like are not needed to be used for carrying out experiments, and the cost is low;
the electric heating plate is started to heat water in the second shell, the temperature sensor detects the temperature of the water in the second shell, the electric heating plate is closed when the temperature of the water reaches the normal body temperature of a human body, the temperature sensor detects the temperature of the water all the time, and the electric heating plate is started when the temperature of the water is lower than the normal body temperature by 0.5 ℃, so that the accuracy of experimental data is ensured;
third, the tackifier is injected into the second shell, the vibration motor is started, the vibration exciting rod of the vibration motor drives the supporting plate to vibrate by expanding the transmission area through the first transmission plate, when the supporting plate vibrates, the vibration area is expanded by matching the four transmission rods with the second transmission plate to drive the second shell to vibrate, so that the tackifier injected into the second shell can be rapidly dispersed into the second shells and the third pipes, at the moment, the viscosity is detected through the viscosity detector, the tackifier is injected at a low speed at intervals in the injection process, and the tackifier is diffused by using the vibration assembly during the intervals, so that the viscosity is ensured not to deviate from the blood viscosity of a human body.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic bottom view of the present invention;
FIG. 3 is a schematic top view of the present invention;
FIG. 4 is a schematic structural view of a second housing according to the present invention;
FIG. 5 is a schematic view of the cross-sectional structure A-A of FIG. 4 according to the present invention.
In the figure: 1. a first housing; 2. a support bar; 3. a support plate; 10. a body assembly; 11. a water pump; 12. a first pipe body; 13. a second tube body; 14. a second housing; 15. a third tube; 20. a vibrating assembly; 21. a vibration motor; 22. a first drive plate; 23. a transmission rod; 24. a second drive plate; 25. a fourth tube body; 26. a fifth pipe body; 27. a valve; 30. a detection component; 31. a viscosity detector; 32. a temperature sensor; 33. a display screen; 34. a controller; 35. a model generator; 36. an electrical heating plate; 37. a separator.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Referring to fig. 1-5, the present invention provides a technical solution: a development method and preliminary pharmacokinetic experiment equipment for in-vitro aptamer comprises a first shell 1, a support rod 2, a support plate 3, a main body component 10, a vibration component 20 and a detection component 30: a main body assembly 10 is installed inside the first shell 1, and the main body assembly 10 comprises a water pump 11, a first pipe 12, a second pipe 13, a second shell 14 and a third pipe 15;
four second shells 14 are symmetrically welded in the first shell 1, a water outlet of the water pump 11 is communicated with one end of the first pipe body 12, the other end of the first pipe body 12 is communicated with one side of one second shell 14, a water inlet of the water pump 11 is communicated with one end of the second pipe body 13, and the other end of the second pipe body 13 is communicated with one side of the other second shell 14; the other two second shells 14 and the first two second shells 14 are communicated with each other through a third pipe body 15;
the vibration assembly 20 is mounted to the upper surface of the support plate 3 and the upper surface of the second housing 14;
the sensing assembly 30 is installed at one side of the water pump 11 and the outside of the second housing 14.
In this embodiment, specifically: four support rods 2 are symmetrically welded on the upper surface of the first shell 1, and support plates 3 are welded on the tops of the four support rods 2; the support rod 2 provides support for the support plate 3, and the support plate 3 provides support for the vibration motor 21 and the first driving plate 22.
In this embodiment, specifically: the vibration assembly 20 comprises a vibration motor 21, a first transmission plate 22, a transmission rod 23, a second transmission plate 24, a fourth pipe body 25, a fifth pipe body 26 and a valve 27;
a first transmission plate 22 is welded on the upper surface of the support plate 3, and a vibration motor 21 is installed in the middle of the upper surface of the first transmission plate 22; the vibration motor 21 is started, and the vibration exciting rod drives the support plate 3 to vibrate by expanding the transmission area through the first transmission plate 22.
In this embodiment, specifically: four transmission rods 23 are welded on the front surface of the supporting plate 3 in an embedded mode, a second transmission plate 24 is welded at the bottom end of each transmission rod 23, and the lower surface of each second transmission plate 24 is welded in the middle of the upper surface of the second shell 14; when the supporting plate 3 vibrates, the vibration area is enlarged by matching the four transmission rods 23 with the second transmission plate 24, so that the second shell 14 is driven to vibrate, and the tackifier injected into the second shell 14 can be rapidly dispersed in the second shells 14 and the third pipe body 15.
In this embodiment, specifically: the upper surface of the second shell 14 is communicated with a fourth pipe 25, and one side of the second shell 14 is communicated with a fifth pipe 26; tackifier, vaccinia virus inflammatory rabbit skin extract injection and substitute bacteria are injected into the second shell 14 through the fourth tube 25, and the substitute bacteria are simulated human lesion cells.
In this embodiment, specifically: the outer sides of the fourth pipe body 25 and the fifth pipe body 26 are respectively provided with a valve 27; the valve 27 controls whether the fourth tube 25 and the fifth tube 26 flow.
In this embodiment, specifically: the detection assembly 30 includes a viscosity detector 31, a temperature sensor 32, a display 33, a controller 34, a model generator 35, an electric heating plate 36 and a partition 37;
the viscosity detector 31 is mounted on the upper surface of a second shell 14, the controller 34 is mounted on one side of the water pump 11, and the signal output end of the viscosity detector 31 is in signal connection with the signal input end of the controller 34; the viscosity detector 31 is used for detecting the viscosity of the water inside the second casing 14, so as to achieve the purpose of optimally simulating the viscosity of the blood of a human body.
In this embodiment, specifically: the electric heating plate 36 is mounted on the bottom wall of the inside of the second shell 14, the temperature sensor 32 is mounted on one side of the second shell 14, the probe of the temperature sensor 32 penetrates through the inside of the second shell 14, and the signal output end of the temperature sensor 32 is in signal connection with the signal input end of the controller 34; through the above arrangement, the electric heating plate 36 heats the water inside the second casing 14, the temperature sensor 32 detects the temperature of the water inside the second casing 14, and when the temperature of the water reaches the normal body temperature of the human body, the electric heating plate 36 is closed.
In this embodiment, specifically: a partition plate 37 is welded on the inner side wall of the second shell 14, and the partition plate 37 is positioned above the electric heating plate 36; the partition 37 isolates the water area electric heating plate 36.
In this embodiment, specifically: a display screen 33 and a model generator 35 are installed on one side of the water pump 11, a signal end of the model generator 35 is in signal connection with a signal end of the controller 34, and a signal input end of the display screen 33 is in signal connection with a signal output end of the controller 34;
a model generator 35 configured to generate model information for display on the display screen 33.
Working principle or structural principle: firstly, a large amount of water source is injected into the second shell 14 through the fourth pipe 25, when the water source is filled in each of the second shell 14 and the third pipe 15, tackifier is injected into the second shell 14, the vibration motor 21 is started, the vibration exciting rod of the vibration motor 21 expands the transmission area through the first transmission plate 22 to drive the support plate 3 to vibrate, when the support plate 3 vibrates, the vibration area is expanded through the four transmission rods 23 in cooperation with the second transmission plate 24 to drive the second shell 14 to vibrate, so that the tackifier injected into the second shell 14 can be rapidly dispersed into the second shells 14 and the third pipe 15, at the moment, the viscosity is detected through the viscosity detector 31, the low-speed interval injection is kept in the tackifier injection process, and the vibration assembly 20 is used for dispersing the tackifier during the interval period, so that the viscosity cannot deviate from the blood viscosity of a human body;
the electric heating plate 36 is started, the electric heating plate 36 heats water in the second shell 14, the temperature sensor 32 detects the temperature of the water in the second shell 14, when the temperature of the water reaches the normal body temperature of a human body, the electric heating plate 36 is closed, the temperature sensor 32 detects the temperature of the water all the time, and when the temperature of the water is lower than the normal body temperature by 0.5 ℃, the electric heating plate 36 is started to ensure that experimental data are accurate;
when the water viscosity reaches the standard, injecting substitute bacteria, simulating the pathological change tissue, starting the vibration motor 21 again, and diffusing the pathological change tissue;
injecting vaccinia virus inflammatory rabbit skin extract injection, starting a water pump 11, simulating heart beating, dispersing the vaccinia virus inflammatory rabbit skin extract injection in a water body through the matching of a first tube body 12, a second tube body 13 and a third tube body 15, generating model information by a model generator 35, sending signals to a controller 34, displaying the model information on a display screen 33, simulating a moving track suitable for the inside of the body and a reaction with a pathological change tissue, performing real-time aptamer internal analysis, realizing preliminary pharmacokinetics, analyzing the reaction suitable for the inside of the body, and performing experiments without expensive instruments or experiments with mice and other experimental bodies, so that the cost is low.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. A development of a method for in-vitro analysis in a standing aptamer and a preliminary pharmacokinetic experiment device comprise a first shell (1), a support rod (2), a support plate (3), a main body component (10), a vibration component (20) and a detection component (30), and are characterized in that: a main body assembly (10) is installed inside the first shell (1), and the main body assembly (10) comprises a water pump (11), a first pipe body (12), a second pipe body (13), a second shell (14) and a third pipe body (15);
four second shells (14) are symmetrically welded in the first shell (1), a water outlet of the water pump (11) is communicated with one end of the first pipe body (12), the other end of the first pipe body (12) is communicated with one side of one second shell (14), a water inlet of the water pump (11) is communicated with one end of the second pipe body (13), and the other end of the second pipe body (13) is communicated with one side of the other second shell (14); the other two second shells (14) and the first two second shells (14) are communicated with each other through a third pipe body (15);
the vibration assembly (20) is mounted on the upper surface of the support plate (3) and the upper surface of the second shell (14);
the detection assembly (30) is installed on one side of the water pump (11) and the outer side of the second shell (14).
2. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 1, characterized in that: the upper surface symmetry welding of first casing (1) has four bracing pieces (2), four the top welding of bracing piece (2) has backup pad (3).
3. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 1, characterized in that: the vibration component (20) comprises a vibration motor (21), a first transmission plate (22), a transmission rod (23), a second transmission plate (24), a fourth pipe body (25), a fifth pipe body (26) and a valve (27);
the upper surface welding of backup pad (3) has first transmission board (22), the upper surface mid-mounting of first transmission board (22) has vibrating motor (21).
4. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 3, characterized in that: the embedded welding of the front surface of backup pad (3) has four transfer lines (23), the bottom welding of transfer line (23) has second driving plate (24), the lower surface welding of second driving plate (24) is in the upper surface middle part of second casing (14).
5. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 3, characterized in that: the upper surface of the second shell (14) is communicated with a fourth pipe body (25), and one side of the second shell (14) is communicated with a fifth pipe body (26).
6. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 3, characterized in that: and valves (27) are arranged on the outer sides of the fourth pipe body (25) and the fifth pipe body (26).
7. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 1, characterized in that: the detection assembly (30) comprises a viscosity detector (31), a temperature sensor (32), a display screen (33), a controller (34), a model generator (35), an electric heating plate (36) and a partition plate (37);
the viscosity detector (31) is arranged on the upper surface of a second shell (14), the controller (34) is arranged on one side of the water pump (11), and the signal output end of the viscosity detector (31) is in signal connection with the signal input end of the controller (34).
8. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 7, characterized in that: the electric heating plate (36) is arranged on the inner bottom wall of the second shell (14), the temperature sensor (32) is arranged on one side of the second shell (14), a probe of the temperature sensor (32) penetrates through the inner part of the second shell (14), and the signal output end of the temperature sensor (32) is in signal connection with the signal input end of the controller (34).
9. The analytical process development and preliminary pharmacokinetic experimental setup within a retro-aptamer according to claim 8, characterized by: a partition plate (37) is welded on the inner side wall of the second shell (14), and the partition plate (37) is located above the electric heating plate (36).
10. The in vitro analytical method development and preliminary pharmacokinetic experimental facility according to claim 7, characterized in that: display screen (33) and model generator (35) are installed to one side of water pump (11), the signal end of model generator (35) and the signal end signal connection of controller (34), the signal input part of display screen (33) and the signal output part signal connection of controller (34).
CN202110976689.1A 2021-08-24 2021-08-24 Development and preliminary pharmacokinetics experimental equipment for in-vivo analysis method of immediately-readjustment Active CN113652349B (en)

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CN202110976689.1A CN113652349B (en) 2021-08-24 2021-08-24 Development and preliminary pharmacokinetics experimental equipment for in-vivo analysis method of immediately-readjustment

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CN202110976689.1A CN113652349B (en) 2021-08-24 2021-08-24 Development and preliminary pharmacokinetics experimental equipment for in-vivo analysis method of immediately-readjustment

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US20170260488A1 (en) * 2014-09-05 2017-09-14 Icahn School Of Medicine At Mount Sinai Automated, multifunctional, engineered cardiac tissue culture and testing bioreactor system
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US20110125286A1 (en) * 2007-07-06 2011-05-26 Clare Selden Multi-functional chamber for housing a biological component
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