CN113647638B - Method for extracting dietary fiber from marine red algae - Google Patents
Method for extracting dietary fiber from marine red algae Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Abstract
The invention discloses a method for extracting dietary fiber from marine red algae, which comprises the following steps: preparing thallus Porphyrae and Sargassum solution from thallus Porphyrae and Sargassum solution; adding Lactobacillus delbrueckii or Lactobacillus plantarum into thallus Porphyrae solution and Sargassum solution, respectively, and fermenting to obtain four fermentation liquids; centrifuging, filtering and drying the four fermentation liquids to obtain four fermentation solids; respectively adding water into the four fermentation solids, respectively adding papain and thermostable amylase under water bath conditions for enzymolysis, adjusting pH, respectively adding cellulase for enzymolysis, inactivating enzyme, centrifuging, and collecting precipitate and filtrate; drying the precipitate to obtain insoluble dietary fiber of marine red algae; concentrating the filtrate, precipitating with ethanol, centrifuging, collecting precipitate, and drying to obtain soluble dietary fiber of marine red algae. The method can effectively improve the extraction yield and quality of the ocean red algae dietary fiber, and promote diversification of the deep-processed seaweed products and multilevel processing technology.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a method for extracting dietary fibers from marine red algae.
Background
Dietary fiber is known as a seventh nutrient, can not be absorbed and digested by human small intestine, but can balance nutrition structure. Dietary fibers can be classified by dissolution characteristics into water-soluble dietary fibers (Soluble dietary fiber, SDF) and water-insoluble dietary fibers (Insolouble dietary fiber, IDF), neither of which are degradable by human digestive enzymes. The insoluble dietary fiber mainly acts on intestinal tracts to generate a mechanical peristaltic effect; whereas soluble dietary fibers perform more metabolic functions, such as affecting carbohydrate and lipid metabolism. The research has proved that the dietary fiber has a plurality of excellent functional characteristics, and particularly has outstanding functions in preventing human gastrointestinal diseases and maintaining intestinal microecology. The balanced dietary fiber composition requires more than 10% of the total dietary fiber content of soluble fiber. The content of soluble dietary fiber in most natural dietary fibers is far below 10%, thereby affecting the application thereof. Therefore, increasing the content of soluble fiber in the total dietary fiber to more than 10% is a key to achieving high values.
Seaweed is an important biological resource rich in dietary fiber in nature, and compared with other dietary fiber resources, the seaweed is low in price, and meanwhile, the relatively simple composition of seaweed also enables the dietary fiber to be extracted and utilized more easily.
At present, the whole laver processing industry in China has the problems of single processing variety, low development and utilization degree, main dry products sold in the market, low added value of products and the like.
At present, the red hair alga processing industry has the problems of expensive raw materials, single product types, low added value and weak competitiveness, has low development and utilization degree, and influences the development of the red hair alga proliferation and cultivation industry.
Disclosure of Invention
In order to solve the problems, the invention provides a method for extracting dietary fibers from marine red algae, which can effectively improve the extraction yield and quality of the marine red algae dietary fibers, develop the high-quality dietary fibers in the marine red algae, effectively promote diversification of algae deep processing products and multilevel processing technology, and promote the rapid development of the algae deep processing industry in China.
To achieve the above object, an embodiment of the present invention proposes, in one aspect, a method of extracting dietary fiber from marine red algae, comprising:
(1) Selecting fresh laver and red algae, cleaning, and drying to constant weight;
(2) Mixing the laver and the rambutan with water respectively at a ratio of 1: 100-3: 100 to obtain a laver solution and a chaetoceros solution;
(3) Adding 2% -5% glucose into the laver and the rambutan solution, sterilizing at 60-65 ℃ for 30min, and cooling to below 37 ℃;
(4) Respectively adding 2% -5% of lactobacillus delbrueckii bacterial liquid or lactobacillus plantarum bacterial liquid into the laver and the chaetoceros solution treated in the step (3) as a fermenting agent, and fermenting for 24-72 hours to obtain laver-lactobacillus delbrueckii fermentation liquid, laver-lactobacillus plantarum fermentation liquid, chaetoceros-lactobacillus delbrueckii fermentation liquid and chaetoceros-lactobacillus plantarum fermentation liquid;
(5) Centrifuging and filtering the four fermentation liquids obtained in the step (4), removing supernatant and lactobacillus thalli, and drying to constant weight to obtain four fermentation solids;
(6) Adding water into the four fermentation solids obtained in the step (5) according to a feed liquid ratio of 1:25-1:150, respectively adding 1.0-2.0% of papain and 0.6% of thermostable amylase for enzymolysis for 1-2 h under a water bath condition of 50 ℃, adjusting pH to 5.0-6.0, respectively adding 1.0-2.0% of cellulase for enzymolysis for 1-2 h, boiling to inactivate enzymes after the enzymolysis reaction is finished, centrifuging, and collecting precipitate and filtrate;
(7) Drying the precipitate obtained in the step (6) to obtain insoluble dietary fiber of the marine red algae; concentrating the filtrate obtained in the step (6), precipitating with 95% ethanol with volume of 4 times for 12h, centrifuging, collecting precipitate, and drying to obtain soluble dietary fiber of marine red algae.
According to the method for extracting dietary fibers from marine red algae, provided by the embodiment of the invention, the laver or the red algae is subjected to fermentation pretreatment by lactobacillus and then subjected to enzymolysis, so that the total dietary fiber extraction rate of the red algae can be effectively improved, and the solubility and the water holding capacity of the dietary fibers can be improved.
The method for extracting dietary fibers from marine red algae provided by the embodiment of the invention further comprises the following characteristics:
optionally, the step (5) further comprises the steps of respectively adding water into the four fermentation solids according to a feed liquid ratio of 1:50-1:100 to obtain a laver-lactobacillus delbrueckii fermentation solution, a laver-lactobacillus plantarum fermentation solution, a chaetoceros-lactobacillus delbrueckii fermentation solution and a chaetoceros-lactobacillus plantarum fermentation solution; and (3) processing the four fermentation solutions by using a high-shear dispersing machine, wherein the dispersing speed is 18000-24000 rpm, the dispersing time is 5-20 min, and centrifuging and drying the dispersed fermentation solutions to constant weight.
Alternatively, in the step (4), the viable count of the Lactobacillus delbrueckii bacteria solution and Lactobacillus plantarum bacteria solution is 1×10 6 ～3×10 10 CFU/g。
Optionally, in the step (6), the feed-liquid ratio is 1:25.
Optionally, in step (6), the amount of papain added is 1.0% and the amount of cellulase added is 1.1%.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 shows the effect of different treatments on the extraction yield of dietary fibers of laver or rambutan according to embodiments of the present invention;
FIG. 2 shows the effect of different treatments on the solubility of laver or rambutan dietary fiber according to embodiments of the present invention;
FIG. 3 shows the effect of various treatments on the maintenance of water in laver or rambutan dietary fiber according to embodiments of the present invention.
Detailed Description
The technical scheme of the invention is described below through specific examples. It is to be understood that the mention of one or more method steps of the present invention does not exclude the presence of other method steps before and after the combination step or that other method steps may be interposed between these explicitly mentioned steps; it should also be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. Moreover, unless otherwise indicated, the numbering of the method steps is merely a convenient tool for identifying the method steps and is not intended to limit the order of arrangement of the method steps or to limit the scope of the invention in which the invention may be practiced, as such changes or modifications in their relative relationships may be regarded as within the scope of the invention without substantial modification to the technical matter.
In order to better understand the above technical solution, exemplary embodiments of the present invention are described in more detail below. While exemplary embodiments of the invention are shown, it should be understood that the invention may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
The test materials adopted by the invention are all common commercial products and can be purchased in the market.
Thermostable amylase, papain, and cellulase were purchased from Sigma, usa; the rest of the chemical reagents are all analytically pure.
The invention will now be described with reference to specific examples, which are intended to be illustrative only and not limiting in any way.
Example 1
Fresh laver and red hair algae are selected, washed and then respectively put into an oven, and dried to constant weight at 60 ℃. Accurately weighing 5.0g of each of the laver and the rambutan, respectively adding distilled water according to a feed liquid ratio of 1:25 (g/mL), respectively adding 1.0% of papain and 0.6% of thermostable amylase at the water bath temperature of 50 ℃ for enzymolysis for 1h, adjusting the pH value to 5.5, respectively adding 1.1% of cellulase for enzymolysis for 1.5h, and boiling for enzyme deactivation for 10min after the enzymolysis reaction is finished. After centrifugation of the enzyme-deactivated sample at 4500rpm for 20min, the filtrate and precipitate were collected, respectively. Drying the precipitate to obtain Insoluble Dietary Fiber (IDF) of thallus Porphyrae and Sargassum prepared by enzyme-assisted extraction method. Concentrating the two filtrates, precipitating with 95% (v/v) ethanol of 4 times volume for 12 hr, centrifuging, and drying to obtain water Soluble Dietary Fiber (SDF) of thallus Porphyrae and Sargassum.
Example 2
Fresh laver and red seaweed are selected, cleaned and then are respectively put into a baking oven, dried at 60 ℃ to constant weight, and then are respectively mixed with purified water in a ratio of 1:50 (g/mL) to obtain laver solution and red seaweed solution. The two solutions are pretreated by using a high shear dispersing machine, the dispersing speed is 20000rpm, the time is 10min, and the dispersed sample is centrifuged at 4000rpm and dried at 60 ℃ to constant weight for standby.
Respectively and accurately weighing 5.0g of each of the pretreated dried laver and red hair alga samples, respectively adding distilled water according to a feed-liquid ratio of 1:25 (g/mL), respectively adding 1.0% of papain and 0.6% of thermostable amylase at the water bath temperature of 50 ℃ for enzymolysis for 1h, adjusting the pH value to 5.5, and then adding 1.1% of cellulase for enzymolysis for 1.5h. After the enzymolysis reaction is finished, boiling to inactivate enzyme for 10min, centrifuging the inactivated sample at 4500rpm for 20min, and collecting filtrate and precipitate respectively. And drying the precipitate to obtain Insoluble Dietary Fibers (IDF) of the laver and the red hair alga, which are prepared by a high-speed shearing dispersion-enzyme-assisted combined extraction method. Concentrating the two filtrates, precipitating with 95% (v/v) ethanol of 4 times volume for 12 hr, centrifuging, and drying to obtain water Soluble Dietary Fiber (SDF) of thallus Porphyrae and Sargassum horneri.
Example 3
Selecting fresh thallus Porphyrae and Sargassum, cleaning, respectively placing into oven, and drying at 60deg.C to constant weight. Mixing thallus Porphyrae and Sargassum with purified water at a feed liquid ratio (g/mL) of 1:100 to obtain thallus Porphyrae solution and Sargassum solution. Adding 2.0% glucose into thallus Porphyrae solution and Sargassum solution respectively, and sterilizing at 65deg.C for 30 min; then cooling to below 37 ℃, and respectively adding 5.0% of lactobacillus delbrueckii bacterial liquid or lactobacillus plantarum bacterial liquid into the laver solution and the rambutan alga solution as a ferment. Fermenting at 37deg.C for 48 hr to obtain four red algae-lactobacillus fermentation solutions, namely thallus Porphyrae-Lactobacillus delbrueckii fermentation broth, thallus Porphyrae-Lactobacillus plantarum fermentation broth, sargassum-Lactobacillus delbrueckii fermentation broth and Sargassum-Lactobacillus plantarum fermentation broth. Respectively centrifuging the four fermentation liquids at 4000rpm for 20min, removing supernatant and lactobacillus thallus, and placing in an oven for drying at 60 ℃ to constant weight to obtain four seaweed fermentation solids: laver-Lactobacillus delbrueckii fermentation solid, laver-Lactobacillus plantarum fermentation solid, sargassum-Lactobacillus delbrueckii fermentation solid, and Sargassum-Lactobacillus plantarum fermentation solid.
Respectively weighing 5.0g of the fermented solids of the laver and the rambutan, adding purified water according to a feed-liquid ratio of 1:25 (g/mL), respectively adding 1.0% of papain and 0.6% of thermostable amylase at the water bath temperature of 50 ℃ for enzymolysis for 1h, adjusting the pH value to 5.5, and adding 1.1% of cellulase for enzymolysis for 1.5h. And after the enzymolysis reaction is finished, boiling and inactivating enzyme for 10min. After centrifugation of the enzyme-deactivated sample at 4500rpm for 20min, the filtrate and precipitate were collected, respectively. And drying the precipitate to obtain Insoluble Dietary Fibers (IDF) of the laver and the red algae prepared by a fermentation-enzyme-assisted combined extraction method. Concentrating the two filtrates, precipitating with 95% (v/v) ethanol of 4 times volume for 12 hr, centrifuging, and drying to obtain water Soluble Dietary Fiber (SDF) of thallus Porphyrae and Sargassum.
Example 4
Selecting fresh thallus Porphyrae and Sargassum, cleaning, respectively placing into oven, and drying at 60deg.C to constant weight. Mixing thallus Porphyrae and Sargassum with purified water at a feed liquid ratio (g/mL) of 1:100 to obtain thallus Porphyrae solution and Sargassum solution. Adding 2.0% glucose into thallus Porphyrae solution and Sargassum solution respectively, and sterilizing at 65deg.C for 30 min; then cooling to below 37 ℃, and respectively adding 5.0% of lactobacillus delbrueckii bacterial liquid or lactobacillus plantarum bacterial liquid into the laver solution and the rambutan alga solution as a ferment. Fermenting at 37deg.C for 48 hr to obtain four red algae-lactobacillus fermentation solutions, namely thallus Porphyrae-Lactobacillus delbrueckii fermentation broth, thallus Porphyrae-Lactobacillus plantarum fermentation broth, sargassum-Lactobacillus delbrueckii fermentation broth and Sargassum-Lactobacillus plantarum fermentation broth. Respectively centrifuging the four fermentation liquids at 4000rpm for 20min, removing supernatant and lactobacillus thallus, and placing in an oven for drying at 60 ℃ to constant weight to obtain four seaweed fermentation solids: laver-Lactobacillus delbrueckii fermentation solid, laver-Lactobacillus plantarum fermentation solid, sargassum-Lactobacillus delbrueckii fermentation solid, and Sargassum-Lactobacillus plantarum fermentation solid. Mixing the fermented solids of thallus Porphyrae and Sargassum with purified water at ratio of 1:50 (g/mL) to obtain four fermentation solutions. Pretreating the sample by using a high shear dispersing machine, wherein the dispersing speed is 20000rpm, the dispersing time is 10min, centrifuging the dispersed sample at 4000rpm, and drying the sample at 60 ℃ to constant weight for later use.
Respectively and accurately weighing 5.0g of dried laver and rambutan fermentation solids after shearing and dispersing pretreatment, adding purified water according to a feed-liquid ratio of 1:25 (g/mL), respectively adding 1.0% of papain and 0.6% of thermostable amylase at a water bath temperature of 50 ℃ for enzymolysis for 1h, adjusting pH to 5.5, and adding 1.1% of cellulase for enzymolysis for 1.5h. And after the enzymolysis reaction is finished, boiling and inactivating enzyme for 10min. After centrifugation of the enzyme-deactivated sample at 4500rpm for 20min, the filtrate and precipitate were collected, respectively. And drying the precipitate to obtain Insoluble Dietary Fibers (IDF) of thallus Porphyrae and Sargassum horneri, which are prepared by fermentation, high-speed shearing dispersion and enzyme-assisted combined extraction method. Concentrating the two filtrates, precipitating with 95% (v/v) ethanol of 4 times volume for 12 hr, centrifuging, and drying to obtain water Soluble Dietary Fiber (SDF) of thallus Porphyrae and Sargassum prepared by fermentation-high speed shearing dispersion-enzyme assisted combined extraction method.
Test examples
To determine the effect of the four extraction methods of examples 1-4 on the extraction yield and quality of dietary fiber from red algae, the effect of each extraction preparation method on Total Dietary Fiber (TDF) extraction yield, solubility, and water retention was now determined.
1. Dietary Fiber (TDF) yield calculation
Dietary Fiber (TDF) yield = [ IDF dry mass (g) +sdf dry mass (g) ]/seaweed sample dry mass (g) ×100.
As a result, as shown in FIG. 1, method 1 shows that the seaweed material was directly dried and then subjected to enzymatic hydrolysis-assisted extraction (corresponding to example 1), method 2 shows that the seaweed material was directly dried and then subjected to high-speed shearing dispersion-enzyme-assisted combination extraction (corresponding to example 2), method 3-D shows that Lactobacillus delbrueckii fermentation-enzyme-assisted combination extraction (corresponding to example 3), method 3-P shows that Lactobacillus plantarum fermentation-enzyme-assisted combination extraction (corresponding to example 3), method 4-D shows that Lactobacillus delbrueckii fermentation-high-speed shearing dispersion-enzyme-assisted combination extraction (corresponding to example 4), and method 4-P shows that Lactobacillus delbrueckii fermentation-high-speed shearing dispersion-enzyme-assisted combination extraction (corresponding to example 4). As can be seen from FIG. 1, compared with other examples, the method for extracting red algae total dietary fiber by using the lactobacillus fermentation-high-speed shearing dispersion-enzyme-assisted combination extraction method in example 4 has the best effect, and the method has the extraction rate of 46.96 + -0.73% (lactobacillus delbrueckii fermentation) and 45.22 + -0.80% (lactobacillus plantarum fermentation) for laver total dietary fiber, which is improved by 81.7-89.5% compared with example 1 total dietary fiber; the extraction rate of the total dietary fiber of the rambutan reaches 45.22 +/-1.67 percent (lactobacillus delbrueckii fermentation) and 41.92+/-1.28 percent (lactobacillus plantarum fermentation), and compared with the total dietary fiber of the embodiment 1, the extraction rate is improved by 81.7-89.5 percent; secondly, the extraction method is a lactobacillus fermentation-enzyme-assisted combination extraction method, and the extraction rate of the method on the total dietary fiber of the laver is up to 39.13 +/-1.57 percent (lactobacillus delbrueckii fermentation) and 37.74+/-0.83 percent (lactobacillus plantarum fermentation), compared with the extraction rate of the total dietary fiber of the embodiment 1, the extraction rate is improved by 47.02-58.68 percent; the extraction rate of the total dietary fiber of the rambutan reaches 40.59+/-1.59 percent (lactobacillus delbrueckii fermentation) and 38.66+/-0.32 percent (lactobacillus plantarum fermentation), and compared with the total dietary fiber of the embodiment 1, the extraction rate is improved by 62.42-70.55 percent.
A large number of researches show that the intake of dietary fiber has positive effects of maintaining the health of the digestive tract, reducing blood fat, reducing blood sugar, improving immunity, controlling weight and the like. In addition, dietary fiber can also be used to improve the organoleptic quality and processing characteristics of foods. The enzyme-assisted extraction method has high yield than the hot water extraction preparation method. Compared with the extraction rate of an enzyme-assisted extraction method, the method adopted by the invention can effectively improve the extraction rate of the total dietary fiber of the red algae by adopting the fermentation-high-speed shearing dispersion-enzyme-assisted combined extraction method, and has a great application prospect in the food industry.
2. Dietary fiber solubility calculation
Solubility: x (%) =idf dry mass (g)/total dietary fiber dry mass (g) ×100.
As shown in FIG. 2, the solubility of the dietary fiber can be obviously improved in the embodiment 3, which can reach 14.50+/-0.56%, and is improved by 19.54% compared with the dietary fiber in the embodiment 1. The solubility of the dietary fibers prepared in example 2 and example 4 was significantly reduced compared to example 1, and it was found that the high-speed shear dispersion pretreatment samples reduced the dietary fiber solubility.
3. Insoluble Dietary Fiber (IDF) water retention assay
Accurately weighing 0.5g of red algae insoluble dietary fiber, respectively placing into 100mL centrifuge tubes, adding 12mL of distilled water, stirring uniformly, placing into a centrifuge after soaking for 24h at room temperature, centrifuging for 30min at 3500r/min, standing for 5min, pouring out supernatant, sucking excessive water in the tubes with filter paper, weighing the residual substances in the tubes, repeating the test for 3 times, and taking an average value.
The water holding capacity is calculated as follows:
water holding capacity (%) = [ sample wet mass (g) -sample dry mass (g) ]/sample dry mass (g) ×100.
As shown in FIG. 3, the water holding capacity of example 3 and example 4 was increased compared with those of example 1 and example 2, wherein the dietary fiber extracted in example 3 was excellent in water holding capacity, and the water holding capacity of the laver dietary fiber prepared in example 3 was as high as 17.90.+ -. 1.16g/g, which is 58.63% higher than that of example 1. The water holding capacity of the obtained rambutan dietary fiber extracted by the lactobacillus delbrueckii fermentation-enzyme-assisted extraction method in the embodiment 3 is 17.48+/-0.69 g/g. The good water retention capacity can prevent food from dehydrating and shrinking to improve the product quality, and the dietary fiber with high water retention capacity is more beneficial to the expansion of food and increases the feeling of satiety of human body.
In summary, according to the method for extracting dietary fibers from marine red algae, provided by the embodiment of the invention, the method is used for carrying out fermentation pretreatment on laver or red algae by lactobacillus and then carrying out enzymolysis, so that the total dietary fiber extraction rate of red algae can be effectively improved, and the solubility and water holding capacity of the dietary fibers can be improved.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms should not be understood as necessarily being directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Further, one skilled in the art can engage and combine the different embodiments or examples described in this specification.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (5)
1. A method of extracting dietary fiber from marine red algae, comprising:
(1) Selecting fresh laver and red algae, cleaning, and drying to constant weight;
(2) Mixing the laver and the rambutan with water respectively at a ratio of 1: 100-3: 100 to obtain a red algae solution and a laver solution;
(3) Adding 2% -5% glucose into the laver solution and the chaetoceros solution, sterilizing at 60-65 ℃ for 30min, and cooling to below 37 ℃;
(4) Respectively adding 2% -5% of lactobacillus delbrueckii bacterial liquid or lactobacillus plantarum bacterial liquid into the laver solution and the chaetoceros solution treated in the step (3) as a fermenting agent, and fermenting for 24-72 hours to obtain laver-lactobacillus delbrueckii fermentation liquid, laver-lactobacillus plantarum fermentation liquid, chaetoceros-lactobacillus delbrueckii fermentation liquid and chaetoceros-lactobacillus plantarum fermentation liquid;
(5) Centrifuging and filtering the four fermentation liquids obtained in the step (4), removing supernatant and lactobacillus thalli, and drying to constant weight to obtain four fermentation solids;
(6) Adding water into the four fermentation solids obtained in the step (5) according to a feed liquid ratio of 1:25-1:150, respectively adding 1.0-2.0% of papain and 0.6% of thermostable amylase for enzymolysis for 1-2 h under a water bath condition of 50 ℃, adjusting pH to 5.0-6.0, respectively adding 1.0-2.0% of cellulase for enzymolysis for 1-2 h, boiling to inactivate enzymes after the enzymolysis reaction is finished, centrifuging, and collecting precipitate and filtrate;
(7) Drying the precipitate obtained in the step (6) to obtain insoluble dietary fiber of the marine red algae; concentrating the filtrate obtained in the step (6), precipitating with 95% ethanol with volume of 4 times for 12h, centrifuging, collecting precipitate, and drying to obtain soluble dietary fiber of marine red algae.
2. The method for extracting dietary fiber from marine red algae according to claim 1, wherein the step (5) further comprises adding water to the four fermentation solids respectively according to a feed liquid ratio of 1:50-1:100 to obtain a laver-lactobacillus helveticus fermentation solid solution, a laver-lactobacillus plantarum fermentation solid solution, a rhodobacter erythropolis-lactobacillus helveticus fermentation solid solution and a rhodobacter erythropolis-lactobacillus plantarum fermentation solid solution; and (3) processing the four fermentation solid solutions by using a high-shear dispersing machine, wherein the dispersing speed is 18000-24000 rpm, the dispersing time is 5-20 min, and centrifuging and drying the dispersed fermentation solid solutions to constant weight.
3. The method for extracting dietary fiber from marine red algae according to claim 1, wherein in the step (4), the viable count of the Lactobacillus delbrueckii bacteria solution and the Lactobacillus plantarum bacteria solution is 1×10 6 ～3×10 10 CFU/g。
4. The method of extracting dietary fiber from marine red algae of claim 1, wherein in step (6), the ratio of feed to liquid is 1:25.
5. The method for extracting dietary fiber from marine red algae according to claim 1, wherein in the step (6), the addition amount of papain is 1.0% and the addition amount of cellulase is 1.1%.
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| CN110074418A (en) * | 2019-05-21 | 2019-08-02 | 山东探克生物科技股份有限公司 | A kind of extracting method of seaweed diet fiber |
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