CN113616581A - Coagulation-age youth collagen recombinant anti-aging formula - Google Patents
Coagulation-age youth collagen recombinant anti-aging formula Download PDFInfo
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 88
- 108010035532 Collagen Proteins 0.000 title claims abstract description 88
- 229920001436 collagen Polymers 0.000 title claims abstract description 84
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 241000251468 Actinopterygii Species 0.000 claims abstract description 35
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 26
- 235000013336 milk Nutrition 0.000 claims abstract description 16
- 239000008267 milk Substances 0.000 claims abstract description 16
- 210000004080 milk Anatomy 0.000 claims abstract description 16
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 15
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 13
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 claims abstract description 13
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 13
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229960002442 glucosamine Drugs 0.000 claims abstract description 13
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 13
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 13
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 13
- 239000011718 vitamin C Substances 0.000 claims abstract description 13
- 239000008367 deionised water Substances 0.000 claims abstract description 10
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 241001046595 Mora moro Species 0.000 claims abstract description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 36
- 235000011837 pasties Nutrition 0.000 claims description 28
- 239000000243 solution Substances 0.000 claims description 26
- 238000003756 stirring Methods 0.000 claims description 24
- 239000000839 emulsion Substances 0.000 claims description 21
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 238000000502 dialysis Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000008213 purified water Substances 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 230000032683 aging Effects 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 238000011049 filling Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 210000000988 bone and bone Anatomy 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 4
- 235000013372 meat Nutrition 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 230000003301 hydrolyzing effect Effects 0.000 claims description 2
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 238000005057 refrigeration Methods 0.000 claims description 2
- 239000002893 slag Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 3
- 238000002156 mixing Methods 0.000 claims 2
- 238000002360 preparation method Methods 0.000 claims 1
- 238000010257 thawing Methods 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 12
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 239000000835 fiber Substances 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 235000004252 protein component Nutrition 0.000 abstract 3
- 235000018102 proteins Nutrition 0.000 abstract 2
- 238000007670 refining Methods 0.000 abstract 1
- 238000005215 recombination Methods 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 241001506047 Tremella Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004833 fish glue Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/986—Milk; Derivatives thereof, e.g. butter
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The invention relates to the technical field of collagen, in particular to a recombinant anti-aging formula of collagen of youth of old people, which comprises deionized water, fish collagen powder, vitamin C, glucosamine, hyaluronic acid and milk, and the components are respectively weighed according to the weight ratio: the collagen protein recombinant anti-aging protein powder for the aged young people is prepared by designing and designing collagen protein components of middle and deep sea cod, the collagen protein components have structures closest to human bodies and are most easily absorbed by organisms, further refining and micronizing the collagen protein components serving as collagen protein raw materials, the molecular weight of the collagen protein raw materials is less than 1000 daltons, and the collagen protein recombinant anti-aging protein powder for the aged young people is high in absorption rate, most easily absorbed by the organisms, high in absorption rate and high in absorption conversion rate, can quickly nourish deep collagen fibers of the skin, has the effect of enabling the skin to be full and elastic, and overcomes the defects that liquid content is low and powder is not easily absorbed to the maximum extent.
Description
Technical Field
The invention relates to the technical field of collagen, in particular to a recombinant anti-aging formula of gel-aged youth collagen.
Background
In the modern society, skin care becomes a necessary course for every female, the first step of skin care is to know the importance of collagen, 70% of components in human skin are collagen which is an important substance basis for forming skin, collagen supports an elastic net for skin, so that the skin is in a healthy and good state of being full of water, moist and glossy, the collagen of a female starts to run off at the age of 20 years, the content decreases year by year, the female enters a peak period of running off at the age of 25 years, the content is less than half of the age of 18 at the age of 40 years, the skin aging process is a process of decomposing and running off a large amount of collagen, the collagen can be supplemented to deeply supplement water, and the skin is effectively cared, the current collagen recombination anti-aging adopts edible foods rich in collagen, for example: tremella, chicken feet, pig trotters, fish glue, pork skin, beef tendon, beans, protein, etc. The dietetic invigoration has large molecular weight, is not easy to absorb, has low absorption rate and low absorption conversion rate, can not quickly nourish deep collagen fibers of skin, enables the skin to be full and elastic, and can not overcome the problem of low liquid content.
In conclusion, the invention solves the existing problems by designing a coagulation-age youth collagen recombinant anti-aging formula.
Disclosure of Invention
The invention aims to provide a recombinant anti-aging formula of gel-aged youth collagen, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the aging youth collagen recombination anti-aging formula comprises deionized water, fish collagen powder, vitamin C, glucosamine, hyaluronic acid and milk, and is respectively weighed according to the weight ratio: 50-60 parts of ionized water, 20-30 parts of fish collagen powder, 15-18 parts of vitamin C, 9-10 parts of glucosamine, 6-8 parts of hyaluronic acid and 20-24 parts of milk, and the method comprises the following specific steps:
s1, respectively weighing deionized water, fish collagen powder, vitamin C, glucosamine and hyaluronic acid according to the weight ratio, adding into a vacuum mixer, stirring at a constant speed, and stirring to form a mixed pasty emulsion A;
s2, standing the mixed pasty solution A obtained in the step S1 in a vacuum environment for 12-16h, then placing the mixed pasty solution A in a vacuum stirrer again, adding milk, and stirring at a constant speed to obtain mixed pasty emulsion B containing the milk;
s3, standing the mixed pasty emulsion B obtained in the step S2 in a vacuum environment for 12-16h, conveying the mixed pasty emulsion B into a high-speed refrigerated centrifuge for centrifugation, and sucking supernatant C;
s4, re-dissolving the supernatant C with dilute acetic acid, filling the dissolved solution into a dialysis bag and putting the bag into dialysis equipment, dialyzing with 0.1% and 0.01% acetic acid solutions for 24 hours, dialyzing with distilled water for 24 hours, changing the dialysate twice every day, and finally obtaining the collagen aqueous solution with high purity;
s5, preparing a pure collagen sample from the purified collagen aqueous solution by adopting a low-temperature freeze drying method for later use;
and S6, micronizing the pure collagen sample in the S5 to obtain the recombinant anti-aging protein powder with the molecular weight less than 1000 daltons, and thus obtaining the gel-age youth collagen.
Preferably, the fish collagen powder is selected from deep sea cod, and the specific manufacturing steps are as follows:
s1, cleaning and sorting, namely selecting fresh fish skin, if the fish skin is frozen, adding purified water into a tank wagon to fully thaw, manually removing impurities, broken meat, fish bones and the like doped in the fish skin, soaking for 10-15min by using NaCO3 with the pH value of 8-9, namely sorting the fish skin, soaking in the purified water and manually cleaning, preparing a washing solution by using the purified water, and removing the impurities, the broken meat, the fish bones and the like doped in the fish skin again;
s2, washing, namely washing the fish skin treated by the S1 to the pH of 7 to 7.5 by using purified water;
and S3, feeding the cleaned fish skin into a hydrolysis tank, wherein the feeding water is purified water, and the material-water ratio is kept at 1:1.2-1: 1.5.
S4, hydrolyzing, namely heating to 100 ℃ by using a jacket and keeping for 20-30 min;
s5, carrying out enzymolysis, namely cooling to 55-60 ℃ by using a vacuum or circulating water system, adding 0.05% of enzyme for hydrolysis under the condition of pH7.5-8.0, stopping hydrolysis until no fish skin can be seen by naked eyes, controlling the enzymolysis time within 2h, and controlling the pH value to be not lower than 6.0 during enzymolysis;
s6, inactivating, namely heating to 90 ℃ to inactivate for 20min after enzymolysis is finished;
s7, coarse filtration, namely adjusting the pH to 6.0-6.5 by using HCl, adding argil accounting for 0.5-1% of the solid content of the feed liquid, feeding the argil into a plate-and-frame filtration chamber for primary filtration, and conveying filtered material slag to a refrigeration house for storage;
s8, concentrating, namely concentrating the filtered clear feed liquid to 30% under the conditions of high vacuum and low temperature, and stopping concentrating;
s9, performing powder spraying, namely performing powder spraying operation, wherein the temperature of an air outlet is lower than 80 ℃ during powder spraying;
s10, packaging, namely packaging according to a protein powder packaging mode for later use.
Preferably, the low temperature condition in S8 is a temperature of less than 80 ℃.
Preferably, the rotation speed of the vacuum stirrer in S1 is 650-700r/min and the stirring is continued for 30 min.
Preferably, the rotation speed of the vacuum stirrer in S2 is 800-.
Preferably, the rotation speed of the high-speed freezing centrifuge in the S3 is 4000r/min and the centrifugation is continued for 30-40 min.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the collagen component structure of the cod in the middle and deep sea of fish is closest to that of a human body and is most easily absorbed by the body as a collagen raw material, and the collagen raw material is further refined and superfinely pulverized to obtain the collagen recombinant anti-aging protein powder with the molecular weight of less than 1000 daltons on the basis, so that the gel age youth collagen recombinant anti-aging protein powder is prepared, has the effects of high absorption rate, high absorption rate and high absorption conversion rate, can quickly nourish deep collagen fibers of skin, enables the skin to be full and elastic, and overcomes the characteristics of low liquid content and difficult absorption of powder to the maximum extent.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Please refer to, the present invention provides a technical solution:
the aging youth collagen recombination anti-aging formula comprises deionized water, fish collagen powder, vitamin C, glucosamine, hyaluronic acid and milk, and is respectively weighed according to the weight ratio: 50-60 parts of ionized water, 20-30 parts of fish collagen powder, 15-18 parts of vitamin C, 9-10 parts of glucosamine, 6-8 parts of hyaluronic acid and 20-24 parts of milk, and the method comprises the following specific steps:
s1, respectively weighing deionized water, fish collagen powder, vitamin C, glucosamine and hyaluronic acid according to the weight ratio, adding into a vacuum mixer, stirring at a constant speed, and stirring to form a mixed pasty emulsion A;
s2, standing the mixed pasty solution A obtained in the step S1 in a vacuum environment for 12-16h, then placing the mixed pasty solution A in a vacuum stirrer again, adding milk, and stirring at a constant speed to obtain mixed pasty emulsion B containing the milk;
s3, standing the mixed pasty emulsion B obtained in the step S2 in a vacuum environment for 12-16h, conveying the mixed pasty emulsion B into a high-speed refrigerated centrifuge for centrifugation, and sucking supernatant C;
s4, re-dissolving the supernatant C with dilute acetic acid, filling the dissolved solution into a dialysis bag and putting the bag into dialysis equipment, dialyzing with 0.1% and 0.01% acetic acid solutions for 24 hours, dialyzing with distilled water for 24 hours, changing the dialysate twice every day, and finally obtaining the collagen aqueous solution with high purity;
s5, preparing a pure collagen sample from the purified collagen aqueous solution by adopting a low-temperature freeze drying method for later use;
and S6, micronizing the pure collagen sample in the S5 to obtain the recombinant anti-aging protein powder with the molecular weight less than 1000 daltons, and thus obtaining the gel-age youth collagen.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Example 1:
step 1, respectively weighing 50 parts of deionized water, 20 parts of fish collagen powder, 15 parts of vitamin C, 9 parts of glucosamine and 6 parts of hyaluronic acid according to the weight ratio, adding the materials into a vacuum stirrer for uniform stirring, stirring the materials for 30min at a rotating speed of 650 plus 700r/min, and stirring the materials to form a mixed paste emulsion A;
step 2, placing the mixed pasty solution A obtained in the step 1 in a vacuum environment for standing for 12-16h, then placing in a vacuum stirrer again, adding 20 parts of milk, stirring at a constant speed, and stirring at a rotation speed of 800-;
step 3, placing the mixed pasty emulsion B obtained in the step 2 in a vacuum environment, standing for 12-16h, conveying the mixed pasty emulsion B into a high-speed refrigerated centrifuge for centrifugation, centrifuging for 30-40min at the rotation speed of 4000r/min, and sucking supernatant C;
step 4, dissolving the supernatant C again by using dilute acetic acid, filling the dissolved solution into a dialysis bag and putting the bag into dialysis equipment, dialyzing the bag for 24 hours by using acetic acid solutions with the mass fractions of 0.1 percent and 0.01 percent respectively, dialyzing the bag for 24 hours by using distilled water, changing the dialyzate twice every day, and finally obtaining the collagen aqueous solution with higher purity;
step 5, preparing the purified collagen aqueous solution into a pure collagen sample by adopting a low-temperature freeze drying method for later use;
and 6, micronizing the pure collagen sample obtained in the step 5 to obtain the gel-age youth collagen recombinant anti-aging protein powder with the molecular weight of less than 1000 daltons.
Example 2:
step 1, respectively weighing 55 parts of deionized water, 25 parts of fish collagen powder, 16.5 parts of vitamin C, 9.5 parts of glucosamine and 7.5 parts of hyaluronic acid according to the weight ratio, adding the materials into a vacuum stirrer, stirring at a constant speed for 30min at a rotation speed of 650 plus materials of 700r/min, and stirring to form a mixed pasty emulsion A;
step 2, placing the mixed pasty solution A obtained in the step 1 in a vacuum environment for standing for 12-16h, then placing in a vacuum stirrer again, adding 22.5 parts of milk, stirring at a constant speed, and stirring at a rotation speed of 800-;
step 3, placing the mixed pasty emulsion B obtained in the step 2 in a vacuum environment, standing for 12-16h, conveying the mixed pasty emulsion B into a high-speed refrigerated centrifuge for centrifugation, centrifuging for 30-40min at the rotation speed of 4000r/min, and sucking supernatant C;
step 4, dissolving the supernatant C again by using dilute acetic acid, filling the dissolved solution into a dialysis bag and putting the bag into dialysis equipment, dialyzing the bag for 24 hours by using acetic acid solutions with the mass fractions of 0.1 percent and 0.01 percent respectively, dialyzing the bag for 24 hours by using distilled water, changing the dialyzate twice every day, and finally obtaining the collagen aqueous solution with higher purity;
step 5, preparing the purified collagen aqueous solution into a pure collagen sample by adopting a low-temperature freeze drying method for later use;
and 6, micronizing the pure collagen sample obtained in the step 5 to obtain the gel-age youth collagen recombinant anti-aging protein powder with the molecular weight of less than 1000 daltons.
Example 3:
step 1, respectively weighing 60 parts of deionized water, 30 parts of fish collagen powder, 18 parts of vitamin C, 10 parts of glucosamine and 8 parts of hyaluronic acid according to the weight ratio, adding the mixture into a vacuum stirrer for uniform stirring, stirring the mixture for 30min at a rotation speed of 650 plus 700r/min, and stirring the mixture to form a mixed paste emulsion A;
step 2, placing the mixed pasty solution A obtained in the step 1 in a vacuum environment for standing for 12-16h, then placing in a vacuum stirrer again, adding 24 parts of milk, stirring at a constant speed, and stirring for 30min at a rotation speed of 800-;
step 3, placing the mixed pasty emulsion B obtained in the step 2 in a vacuum environment, standing for 12-16h, conveying the mixed pasty emulsion B into a high-speed refrigerated centrifuge for centrifugation, centrifuging for 30-40min at the rotation speed of 4000r/min, and sucking supernatant C;
step 4, dissolving the supernatant C again by using dilute acetic acid, filling the dissolved solution into a dialysis bag and putting the bag into dialysis equipment, dialyzing the bag for 24 hours by using acetic acid solutions with the mass fractions of 0.1 percent and 0.01 percent respectively, dialyzing the bag for 24 hours by using distilled water, changing the dialyzate twice every day, and finally obtaining the collagen aqueous solution with higher purity;
step 5, preparing the purified collagen aqueous solution into a pure collagen sample by adopting a low-temperature freeze drying method for later use;
and 6, micronizing the pure collagen sample obtained in the step 5 to obtain the gel-age youth collagen recombinant anti-aging protein powder with the molecular weight of less than 1000 daltons.
After the recombinant anti-aging protein powder of the collagen of youth of the coagulation years prepared from the different components in the embodiments 1, 2 and 3 is analyzed by a special instrument, the recombinant anti-aging protein powder of the collagen of the youth of the coagulation years prepared by matching in the embodiment 2 is most easily absorbed by organisms, has the highest absorption rate and high absorption conversion rate, can quickly nourish deep collagen fibers of skin, enables the skin to be full and elastic, overcomes the defects of low liquid content and difficult powder absorption to the greatest extent, and achieves better balance.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The aging youth collagen recombinant anti-aging formula is characterized by comprising deionized water, fish collagen powder, vitamin C, glucosamine, hyaluronic acid and milk, wherein the collagen recombinant anti-aging formula is respectively weighed according to the weight ratio: 50-60 parts of ionized water, 20-30 parts of fish collagen powder, 15-18 parts of vitamin C, 9-10 parts of glucosamine, 6-8 parts of hyaluronic acid and 20-24 parts of milk, and the method comprises the following specific steps:
s1, respectively weighing deionized water, fish collagen powder, vitamin C, glucosamine and hyaluronic acid according to the weight ratio, adding into a vacuum mixer, stirring at a constant speed, and stirring to form a mixed pasty emulsion A;
s2, standing the mixed pasty solution A obtained in the step S1 in a vacuum environment for 12-16h, then placing the mixed pasty solution A in a vacuum stirrer again, adding milk, and stirring at a constant speed to obtain mixed pasty emulsion B containing the milk;
s3, standing the mixed pasty emulsion B obtained in the step S2 in a vacuum environment for 12-16h, conveying the mixed pasty emulsion B into a high-speed refrigerated centrifuge for centrifugation, and sucking supernatant C;
s4, re-dissolving the supernatant C with dilute acetic acid, filling the dissolved solution into a dialysis bag and putting the bag into dialysis equipment, dialyzing with 0.1% and 0.01% acetic acid solutions for 24 hours, dialyzing with distilled water for 24 hours, changing the dialysate twice every day, and finally obtaining the collagen aqueous solution with high purity;
s5, preparing a pure collagen sample from the purified collagen aqueous solution by adopting a low-temperature freeze drying method for later use;
and S6, micronizing the pure collagen sample in the S5 to obtain the recombinant anti-aging protein powder with the molecular weight less than 1000 daltons, and thus obtaining the gel-age youth collagen.
2. The recombinant aging-resistant formula of collagen of aged youth according to claim 1, wherein the fish collagen powder is selected from deep sea cod, and the specific preparation steps are as follows:
s1, cleaning and sorting, wherein fresh fish skin, such as frozen fish skin, is selected, purified water is added into a tank wagon for full thawing, impurities, minced meat, fish bones and the like doped in the fish skin are manually removed, and NaCO with pH of 8-9 is used3Soaking for 10-15min, namely picking out, soaking in purified water, manually cleaning, preparing a cleaning solution by using the purified water, and removing impurities, minced meat, fish bones and the like doped in the fish skin;
s2, washing, namely washing the fish skin treated by the S1 to the pH of 7 to 7.5 by using purified water;
and S3, feeding the cleaned fish skin into a hydrolysis tank, wherein the feeding water is purified water, and the material-water ratio is kept at 1:1.2-1: 1.5.
S4, hydrolyzing, namely heating to 100 ℃ by using a jacket and keeping for 20-30 min;
s5, carrying out enzymolysis, namely cooling to 55-60 ℃ by using a vacuum or circulating water system, adding 0.05% of enzyme for hydrolysis under the condition of pH7.5-8.0, stopping hydrolysis until no fish skin can be seen by naked eyes, controlling the enzymolysis time within 2h, and controlling the pH value to be not lower than 6.0 during enzymolysis;
s6, inactivating, namely heating to 90 ℃ to inactivate for 20min after enzymolysis is finished;
s7, coarse filtration, namely adjusting the pH to 6.0-6.5 by using HCl, adding argil accounting for 0.5-1% of the solid content of the feed liquid, feeding the argil into a plate-and-frame filtration chamber for primary filtration, and conveying filtered material slag to a refrigeration house for storage;
s8, concentrating, namely concentrating the filtered clear feed liquid to 30% under the conditions of high vacuum and low temperature, and stopping concentrating;
s9, performing powder spraying, namely performing powder spraying operation, wherein the temperature of an air outlet is lower than 80 ℃ during powder spraying;
s10, packaging, namely packaging according to a protein powder packaging mode for later use.
3. The recombinant aging-resistant formula of collagen of aged youth according to claim 2, wherein the temperature of the S8 is lower than 80 ℃.
4. The recombinant anti-aging formulation of collagen from youth of old people as claimed in claim 1, wherein the rotation speed of the vacuum mixer in S1 is 650-700r/min and the mixing is continued for 30 min.
5. The recombinant anti-aging formulation of collagen from youth of old people as claimed in claim 1, wherein the rotation speed of the vacuum mixer in S2 is 800-1000r/min and the mixing is continued for 30 min.
6. The recombinant aging-resistant formulation of collagen of youth of old people as claimed in claim 1, wherein the rotation speed of the high speed refrigerated centrifuge in S3 is 4000r/min and the centrifugation lasts 30-40 min.
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