CN113614113A - 含有荧光蛋白片段的融合蛋白及其用途 - Google Patents

含有荧光蛋白片段的融合蛋白及其用途 Download PDF

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CN113614113A
CN113614113A CN202080023378.7A CN202080023378A CN113614113A CN 113614113 A CN113614113 A CN 113614113A CN 202080023378 A CN202080023378 A CN 202080023378A CN 113614113 A CN113614113 A CN 113614113A
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fusion protein
peptide
protein
another preferred
amino acid
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吴松
张振山
刘慧玲
陈卫
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Ningbo Kunpeng Biotech Co Ltd
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Ningbo Kunpeng Biotech Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
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  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

提供了含有荧光蛋白片段的融合蛋白及其用途。本发明的融合蛋白具有(P1‑L1)s‑A1‑(X)n‑A2‑(L2‑P2)t所示的结构，其中(X)n或A1‑(X)n可促进融合目的肽的折叠和表达，并且可提高融合蛋白的溶解性并降低融合蛋白的分子间相互作用。融合蛋白中的(X)n或A1‑(X)n可以被酶切成多个长度远小于目的肽的短肽，有利于其与目的肽分离，使目的肽纯化更方便。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN202080023378.7A 2019-03-19 2020-03-18 含有荧光蛋白片段的融合蛋白及其用途 Pending CN113614113A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201910210102.9A CN111718417B (zh) 2019-03-19 2019-03-19 含有荧光蛋白片段的融合蛋白及其用途
CN2019102101029 2019-03-19
PCT/CN2020/080036 WO2020187270A1 (zh) 2019-03-19 2020-03-18 含有荧光蛋白片段的融合蛋白及其用途

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CN113614113A true CN113614113A (zh) 2021-11-05

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CN202080023378.7A Pending CN113614113A (zh) 2019-03-19 2020-03-18 含有荧光蛋白片段的融合蛋白及其用途

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US (1) US20220162282A1 (zh)
EP (1) EP3950719A4 (zh)
JP (1) JP7266325B2 (zh)
CN (2) CN111718417B (zh)
AU (1) AU2020242472A1 (zh)
BR (1) BR112021018686A2 (zh)
CA (1) CA3134167A1 (zh)
WO (1) WO2020187270A1 (zh)

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Publication number Priority date Publication date Assignee Title
CN113773398B (zh) * 2020-06-10 2023-05-23 宁波鲲鹏生物科技有限公司 一种德谷胰岛素衍生物及其应用
CN114075295B (zh) * 2020-08-19 2024-05-24 苏州鲲鹏生物技术有限公司 一种Boc-人胰岛素融合蛋白包涵体的高效复性液及其复性方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1886420A (zh) * 2003-10-24 2006-12-27 加利福尼亚大学 自我组装脱落荧光蛋白系统
CN104619726A (zh) * 2012-03-23 2015-05-13 苏州鲲鹏生物技术有限公司 由超折叠绿色荧光蛋白构成的融合蛋白及其用途
WO2017216300A1 (fr) * 2016-06-15 2017-12-21 Universite Claude Bernard Lyon 1 Vecteurs et banques de cellules pour une methode de criblage d'interaction proteine-proteine
CN108779152A (zh) * 2016-01-27 2018-11-09 建国大学校产学协力团 包含抗菌肽的不溶性融合蛋白以及利用上述不溶性融合蛋白的抗菌肽制造方法

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
EP1373889A2 (en) * 2000-07-31 2004-01-02 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
JP2002262873A (ja) * 2001-03-06 2002-09-17 Inst Of Physical & Chemical Res タンパク質ドメインの製造方法及びその方法により得られたドメインを用いるタンパク質の立体構造解析方法
DK2393828T3 (en) * 2009-02-03 2017-01-23 Amunix Operating Inc Extended recombinant polypeptides and compositions comprising same
WO2010104114A1 (ja) * 2009-03-10 2010-09-16 独立行政法人産業技術総合研究所 ポリペプチドライブラリーを調製する方法
WO2011123813A2 (en) * 2010-04-02 2011-10-06 Amunix Operating Inc. Binding fusion proteins, binding fusion protein-drug conjugates, xten-drug conjugates and methods of making and using same
CN102618548B (zh) * 2011-01-27 2013-06-19 华东理工大学 一种次氯酸的新型绿色荧光变体蛋白探针及其编码序列
CN102504022A (zh) * 2011-11-30 2012-06-20 苏州元基生物技术有限公司 含有保护赖氨酸的胰岛素原及使用其制备胰岛素的方法
AU2016357091A1 (en) * 2015-11-20 2018-06-07 Duke University Glucose biosensors and uses thereof
WO2017094885A1 (ja) * 2015-12-04 2017-06-08 国立大学法人東京大学 リガンド蛍光センサータンパク質とその使用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1886420A (zh) * 2003-10-24 2006-12-27 加利福尼亚大学 自我组装脱落荧光蛋白系统
CN104619726A (zh) * 2012-03-23 2015-05-13 苏州鲲鹏生物技术有限公司 由超折叠绿色荧光蛋白构成的融合蛋白及其用途
CN108779152A (zh) * 2016-01-27 2018-11-09 建国大学校产学协力团 包含抗菌肽的不溶性融合蛋白以及利用上述不溶性融合蛋白的抗菌肽制造方法
WO2017216300A1 (fr) * 2016-06-15 2017-12-21 Universite Claude Bernard Lyon 1 Vecteurs et banques de cellules pour une methode de criblage d'interaction proteine-proteine

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
XUDONG WU等: "A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag", JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 2009, pages 1 - 8 *

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Publication number Publication date
EP3950719A1 (en) 2022-02-09
AU2020242472A1 (en) 2021-11-11
WO2020187270A1 (zh) 2020-09-24
BR112021018686A2 (pt) 2021-11-23
CA3134167A1 (en) 2020-09-24
JP7266325B2 (ja) 2023-04-28
US20220162282A1 (en) 2022-05-26
JP2022528151A (ja) 2022-06-08
EP3950719A4 (en) 2023-01-11
CN111718417A (zh) 2020-09-29
CN111718417B (zh) 2022-10-14

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