CN113614097A - 高强度标记的反应物组合物和测序方法 - Google Patents

高强度标记的反应物组合物和测序方法 Download PDF

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Publication number
CN113614097A
CN113614097A CN202080023453.XA CN202080023453A CN113614097A CN 113614097 A CN113614097 A CN 113614097A CN 202080023453 A CN202080023453 A CN 202080023453A CN 113614097 A CN113614097 A CN 113614097A
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nucleic acid
molecule
labeled nucleotide
luminescent
molecules
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Chinese (zh)
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乔纳森·M·罗斯伯格
杰瑞米·雷克伊
布莱恩·瑞德
施兴华
黄海冬
大卫·达德
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Quantum Si Inc
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Quantum Si Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/103—Nucleic acid detection characterized by the use of physical, structural and functional properties luminescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202080023453.XA 2019-01-23 2020-01-23 高强度标记的反应物组合物和测序方法 Pending CN113614097A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962795932P 2019-01-23 2019-01-23
US62/795,932 2019-01-23
PCT/US2020/014856 WO2020154546A1 (en) 2019-01-23 2020-01-23 High intensity labeled reactant compositions and methods for sequencing

Publications (1)

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CN113614097A true CN113614097A (zh) 2021-11-05

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US (2) US11613772B2 (https=)
EP (1) EP3914603A1 (https=)
JP (1) JP7618563B2 (https=)
KR (1) KR20210118862A (https=)
CN (1) CN113614097A (https=)
AU (1) AU2020210940A1 (https=)
BR (1) BR112021013787A2 (https=)
CA (1) CA3127102A1 (https=)
MX (1) MX2021008867A (https=)
WO (1) WO2020154546A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3069985A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
MX2021008867A (es) * 2019-01-23 2021-08-19 Quantum Si Inc Composiciones reactantes marcadas de intensidad alta y metodos de secuenciamiento.
WO2022159193A1 (en) * 2021-01-20 2022-07-28 The Regents Of The University Of California Proximity-dependent photoactivation
JP7787670B2 (ja) * 2021-08-31 2025-12-17 富士フイルム株式会社 化合物及びこれを用いた標識生体物質
EP4397672A4 (en) * 2021-08-31 2025-02-26 FUJIFILM Corporation Compound and labeled biomaterial using same
WO2023137314A1 (en) * 2022-01-12 2023-07-20 Quantum-Si Incorporated Labeled binding reagents and methods of use thereof
WO2026020143A1 (en) * 2024-07-19 2026-01-22 Prolynx Llc Extended-release conjugates of lipidated peptides, and uses thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5594117A (en) * 1994-08-25 1997-01-14 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties and associated methods of synthesis and use
CN101076537A (zh) * 2003-11-19 2007-11-21 阿莱洛吉克生物科学公司 标记有多个荧光团的寡核苷酸
CN101268200A (zh) * 2005-07-20 2008-09-17 Lgc有限公司 寡核苷酸
US20090233302A1 (en) * 2008-03-13 2009-09-17 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
WO2010117420A2 (en) * 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
WO2012027625A2 (en) * 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Scaffold-based polymerase enzyme substrates
CN102782159A (zh) * 2010-06-02 2012-11-14 财团法人工业技术研究院 将核酸进行测序之组合物与方法
US20150005069A1 (en) * 2012-02-14 2015-01-01 DeNA Co., Ltd. Computing of social game

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
DE4435728A1 (de) 1994-01-19 1995-07-20 Boehringer Mannheim Gmbh Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
DE19782089T1 (de) 1996-10-29 1999-12-23 Univ Nebraska At Lincoln Linco Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang
GB9716231D0 (en) 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
JP3813818B2 (ja) 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ オリゴヌクレオチドおよびｄｎａ分子のヌクレオチド配列の決定方法
US6153442A (en) 1998-05-20 2000-11-28 Dade Behring Inc. Reagents and methods for specific binding assays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
WO2000036151A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A heterogeneous assay for pyrophosphate detection
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1681357A3 (en) 1999-05-19 2006-07-26 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423536B1 (en) 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
JP2004516810A (ja) 2000-06-07 2004-06-10 リ−コール インコーポレーティッド 電荷スイッチヌクレオチド
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US6627748B1 (en) * 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
AU2002258502A1 (en) 2001-03-12 2002-09-24 Affymetrix, Inc. Nucleic acid labeling compounds
JP4318296B2 (ja) 2001-08-29 2009-08-19 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 標識ヌクレオシドポリホスフェート
US9389227B2 (en) 2001-09-05 2016-07-12 Posco Solid substrate comprising array of dendrons and methods for using the same
AU2002366046A1 (en) 2001-10-19 2003-06-10 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US20040054162A1 (en) 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
DE10154291B4 (de) 2001-11-05 2005-05-19 Hain Lifescience Gmbh Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003089670A2 (en) 2002-04-22 2003-10-30 Lawler Joseph F Jr Reagents for monitoring nuclei acid amplification and methods of using same
EP1509624A2 (en) 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP1725572B1 (de) 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
GB0422551D0 (en) 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
EP1920072A2 (en) 2005-07-01 2008-05-14 Dako Denmark A/S In situ hybridization detection method
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
EP1960550B1 (en) 2005-12-12 2010-09-15 The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services Probe for nucleic acid sequencing and methods of use
CN101346472B (zh) 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
CN104524596B (zh) 2006-01-20 2018-10-09 星药股份有限公司 经修饰的大分子
WO2007106900A2 (en) 2006-03-15 2007-09-20 The Trustees Of The University Of Pennsylvania Quantitative molecular probes
US8084734B2 (en) 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
GB0625678D0 (en) 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
CN101434988B (zh) 2007-11-16 2013-05-01 深圳华因康基因科技有限公司 一种高通量寡核苷酸测序方法
WO2009091847A2 (en) 2008-01-14 2009-07-23 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
MX2010010600A (es) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Composiciones y metodos para secuenciacion de acidos nucleicos.
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
WO2009145820A2 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
WO2009145818A1 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Single molecule loading methods and compositions
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
US8252910B2 (en) 2008-11-19 2012-08-28 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
JP2012509675A (ja) 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子ｒｎａを検出するための組成物および方法、ならびにその使用
WO2010065322A1 (en) 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
KR101063981B1 (ko) 2009-01-12 2011-09-14 서강대학교산학협력단 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법
DE102009013653B4 (de) 2009-03-18 2014-09-18 Bruker Daltonik Gmbh Protein-Sequenzierung mit MALDI-Massenspektrometrie
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
JP6073684B2 (ja) 2010-01-19 2017-02-01 シリゲン セカンド リミテッド 指向性バイオマーカーシグナル増幅のための新規の試薬
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
CN103003449A (zh) 2010-05-06 2013-03-27 艾比斯生物科学公司 集成样品制备系统和稳定的酶混合物
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
CN102329884B (zh) 2011-10-20 2013-05-08 东南大学 两核苷酸同时合成dna测序方法及其应用
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9435810B2 (en) 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
EP3008094B1 (en) 2013-06-14 2018-12-26 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US9957291B2 (en) 2013-08-05 2018-05-01 Pacific Biosciences Of California, Inc. Protected fluorescent reagent compounds
JP2015073523A (ja) 2013-10-11 2015-04-20 国立大学法人 東京大学 核酸の検出方法、検出プローブ、マイクロアレイ、核酸検出キット、核酸‐検出プローブ‐捕捉プローブ複合体、核酸固定化担体、及び流体デバイス
CN103866010B (zh) 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
US9970049B2 (en) 2014-04-15 2018-05-15 The United States Of America, As Represented By The Secretary Of The Navy Use of modular nucleic acid scaffolds to create nanoscale energy harvesting and focusing arrays
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3258952A4 (en) 2015-02-04 2018-12-05 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
CA2986237A1 (en) 2015-05-20 2016-11-24 Quantum-Si Incorporated Method of determining the sequence of a nucleic acid using time resolved luminescence
EP3098322A1 (en) 2015-05-29 2016-11-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method to detect activity of a polymerase
CN105001292A (zh) 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
EP3371194B1 (en) 2015-10-16 2020-12-30 Andrew Emili Protein sequencing method
EP3377656B1 (en) 2015-11-18 2025-07-02 Pacific Biosciences of California, Inc. Loading nucleic acids onto substrates
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
BR112018073378A2 (pt) 2016-05-20 2019-03-06 Quantum-Si Incorporated composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos
CA3069985A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
EP3655408B1 (en) 2017-08-24 2021-07-14 The U.S.A. As Represented By The Secretary, Department Of Health And Human Services Conformational restriction of cyanine fluorophores in far-red and near-ir range
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
US10836798B2 (en) 2018-11-08 2020-11-17 Government Of The United States Of America, As Represented By The Secretary Of Commerce Amino acid-specific binder and selectively identifying an amino acid
MX2021008867A (es) 2019-01-23 2021-08-19 Quantum Si Inc Composiciones reactantes marcadas de intensidad alta y metodos de secuenciamiento.

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5594117A (en) * 1994-08-25 1997-01-14 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties and associated methods of synthesis and use
CN101076537A (zh) * 2003-11-19 2007-11-21 阿莱洛吉克生物科学公司 标记有多个荧光团的寡核苷酸
CN101268200A (zh) * 2005-07-20 2008-09-17 Lgc有限公司 寡核苷酸
US8383791B1 (en) * 2005-07-20 2013-02-26 Lgc Limited Oligonucleotides
US20090233302A1 (en) * 2008-03-13 2009-09-17 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
WO2010117420A2 (en) * 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
CN102782159A (zh) * 2010-06-02 2012-11-14 财团法人工业技术研究院 将核酸进行测序之组合物与方法
WO2012027625A2 (en) * 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Scaffold-based polymerase enzyme substrates
US20150005069A1 (en) * 2012-02-14 2015-01-01 DeNA Co., Ltd. Computing of social game

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