CN113604350B - Automatic change immune cell culture medium partial shipment device - Google Patents
Automatic change immune cell culture medium partial shipment device Download PDFInfo
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- CN113604350B CN113604350B CN202110927218.1A CN202110927218A CN113604350B CN 113604350 B CN113604350 B CN 113604350B CN 202110927218 A CN202110927218 A CN 202110927218A CN 113604350 B CN113604350 B CN 113604350B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The invention relates to an automatic immune cell culture medium split charging device, which comprises: the culture dish comprises a supporting bottom plate, a main stepping motor, a turntable mechanism, a culture dish and a dish cover. The main stepping motor is fixedly connected to the supporting bottom plate. The turntable mechanism comprises a limiting ring, a supporting ring and a turntable body. An automated immunocyte medium split charging device further comprises: the culture medium feeding mechanism and the transmission cylinder for vertically stacking the culture dishes. The vertical setting of conduction section of thick bamboo is in the upper left side of carousel body, and conduction section of thick bamboo and supporting baseplate fixed connection simultaneously. The culture medium split charging device has the advantages that the culture medium is automatically split charged into the culture dishes, the split charged culture dishes are automatically charged into the storage device, manual processing is not needed, and the culture medium split charging efficiency is improved. And simultaneously, automatically sterilizing and disinfecting the culture dish and the dish cover before split charging.
Description
Technical Field
The invention relates to the technical field of immune cell culture medium split charging devices, in particular to an automatic immune cell culture medium split charging device.
Background
Typically, immune cells need to be cultured by a medium, which is typically contained in a petri dish. The culture dish is a glass container. When the culture medium is packaged into the culture dish, the culture dish is placed by manual operation, then the culture medium is packaged into the culture dish, and the efficiency is low due to manual sealing. Meanwhile, before the culture medium is split, the culture dish needs to be sterilized and then the culture medium is filled, so that the split-charging type culture dish is not convenient and fast.
Disclosure of Invention
The invention aims to provide an automatic immune cell culture medium split charging device so as to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an automated immunocyte medium dispensing apparatus, comprising: the culture dish comprises a supporting bottom plate, a main stepping motor, a turntable mechanism, a culture dish and a dish cover. The main stepping motor is fixedly connected to the supporting bottom plate. The turntable mechanism comprises a limiting ring, a supporting ring and a turntable body. The turntable body is fixedly connected to the main shaft end of the main stepping motor. The supporting rings are arranged at the edge of the turntable body at equal intervals. The limiting ring is arranged on the upper side of the supporting ring.
An automated immunocyte medium split charging device further comprises: the culture medium feeding mechanism and the transmission cylinder for vertically stacking the culture dishes. The vertical setting of conduction section of thick bamboo is in the upper left side of carousel body, and conduction section of thick bamboo and supporting baseplate fixed connection simultaneously.
The culture medium feeding mechanism comprises: the device comprises a vertical cylinder, a first electric telescopic rod, a butt joint hole, a pressure sensing switch, a linkage mechanism, a piston injection cylinder, a piston push rod and a first countdown starting switch. The vertical section of thick bamboo is vertical to be set up in the right-hand member upside of carousel body to vertical section of thick bamboo and supporting baseplate fixed connection. The first electric telescopic rod is vertically and fixedly connected to the upper side of the right end of the supporting bottom plate. The butt joint hole is arranged at the bottom of the culture dish. The pressure sensing switch is mounted to the inner wall of the vertical cylinder. The piston injection cylinder is horizontally and fixedly connected to the outer wall of the upper end of the vertical cylinder. The piston push rod is connected to the inner side of the piston injection cylinder in a sliding fit manner.
The linkage mechanism comprises: the device comprises a linkage ring, a linkage rod, a connecting guide rail, an auxiliary stepping motor, a gear and a rack. The linkage ring is sleeved outside the vertical cylinder. The linkage rod is movably connected to the outer end of the piston push rod through a hinge, and the tail end of the linkage rod is movably connected to the linkage ring through a hinge. The connecting guide rail is vertically and fixedly connected to the left outer wall of the vertical barrel. The auxiliary stepping motor is connected to the connecting guide rail in a sliding way, and the pressure sensing switch is electrically connected with a starting circuit of the auxiliary stepping motor. The auxiliary stepping motor is fixedly connected with the linkage ring. The gear is fixedly connected to the main shaft end of the auxiliary stepping motor. The rack is vertically and fixedly connected to the upper end face of the turntable body. The gear is meshed with the rack. The first countdown starting switch is mounted to the inner wall of the vertical cylinder. The first countdown starting switch is electrically connected with a contraction control circuit of the first electric telescopic rod.
As a further scheme of the invention: the conductive cylinder includes: the connecting rope and the gravity pressing block. The gravity pressing block is connected to the upper side of the conduction cylinder through a connecting rope.
As a further scheme of the invention: the outer wall of the piston injection cylinder is provided with a one-way valve for adding culture medium ingredients.
As a further scheme of the invention: the culture medium feeding mechanism further comprises: and a capping mechanism. The capping mechanism includes: put box, drive wheel, conveyer belt, second countdown start switch and removable top cap. The placing box is horizontally connected to the outer wall of the right side of the vertical cylinder, and is communicated with the inside of the vertical cylinder. The driving wheels are arranged at the inner sides of the left end and the right end of the placement box in pairs, and a stepping driving motor is arranged at the driving wheels. The conveyor belt is arranged between the driving wheels in a surrounding way. The dish covers are transversely placed on the conveyor belt at equal intervals. The detachable top cover is arranged on the upper side of the placement box. The second countdown start switch is installed to the inner wall department of vertical section of thick bamboo to the first countdown start switch position parallel and level of second countdown start switch. And the second countdown starting switch is electrically connected with a stepping driving motor arranged on the driving wheel.
As a further scheme of the invention: the upper port of the culture dish is provided with a magnet ring. The lower edge of the dish cover is provided with an iron ring matched with the magnet ring.
As a further scheme of the invention: the turntable mechanism further includes: the device comprises a connecting sleeve, a lifting column and a connecting spring. The connecting sleeve is connected to the lower side of the supporting ring. The lifting column is vertically connected to the inner side of the connecting sleeve in a sliding manner. The connecting spring is sleeved at the lifting column, and two ends of the connecting spring are respectively and fixedly connected to the lower end of the lifting column and the connecting sleeve. The first electric telescopic link includes: the first trigger button switch and the first elastic rubber sheet. The first elastic rubber sheet is connected to the outer wall of the fixed end of the first electric telescopic rod. The first trigger button switch is arranged at the first elastic rubber sheet.
As a further scheme of the invention: the upper end of the lifting column is provided with an elastic rubber body.
As a further scheme of the invention: an automated immunocyte medium split charging device further comprises: a high-temperature airflow sterilization mechanism. The high-temperature airflow sterilization mechanism comprises a high-pressure air pump and an electric heating wire. The high-pressure air pump is installed to the upper end of vertical section of thick bamboo to the end of giving vent to anger of high-pressure air pump is connected to the upper end inboard of vertical section of thick bamboo vertically downwards. The electric heating wire is arranged at the inner side of the air outlet end of the high-pressure air pump. The electric heating wire and the high-pressure air pump are electrically connected with the first trigger button switch.
As a further scheme of the invention: an automated immunocyte medium split charging device further comprises: and (5) recycling and boxing the mechanism. The recycling and boxing mechanism comprises: the device comprises a second electric telescopic rod, an electromagnet, a recovery cylinder, a second elastic rubber sheet, a shrinkage starting switch, an ejector rod, a second trigger button switch, a sliding rod and a reset spring. The second electric telescopic rod is horizontally and fixedly connected to the upper part of the front end of the supporting bottom plate. The electromagnet is fixedly connected to the telescopic end of the second electric telescopic rod. The recovery cylinder is arranged on the upper side of the front end of the supporting bottom plate. The second elastic rubber piece is arranged below the front side edge of the turntable body and is fixedly connected to the outer wall of the fixed end of the second electric telescopic rod through the rod body. The shrinkage start switch is arranged at the second elastic rubber sheet and is connected with a shrinkage control circuit of the second electric telescopic rod and the electromagnetic iron. The limiting ring is an iron ring. The slide bar is horizontally and fixedly connected to the limiting ring, and horizontally slides and penetrates through the inner side of the turntable body. The reset spring is connected between the slide bar and the turntable body. The ejector rod is horizontally and fixedly connected to the electromagnet. The second trigger button switch is mounted to the fixed end of the second electric telescopic rod, and is electrically connected with the extension control circuit of the second electric telescopic rod.
As a further scheme of the invention: the recovery cylinder includes: a pallet and a support spring. The supporting plate is horizontally arranged on the inner side of the recovery cylinder. The supporting spring is vertically and fixedly connected between the supporting plate and the inner bottom of the recovery cylinder.
Compared with the prior art, the invention has the beneficial effects that: the culture medium is automatically split-packed into the culture dishes, and the split-packed culture dishes are automatically loaded into the storage device without manual treatment, so that the split-packed culture medium efficiency is improved.
Mainly rely on the carousel body rotation, with the culture dish transmission to culture medium throwing mechanism department one by one and carry out the partial shipment of culture medium to automatic closing cap carries out after the partial shipment culture medium. Then rely on retrieving vanning mechanism and make the culture dish of the culture medium of adorning on the carousel body break away from automatically and get off and pack into storage device, realize that the position that the culture dish was put to the card on the carousel body is vacated to be convenient for next empty culture dish card goes into, so realize that whole device partial shipment repeatedly.
And simultaneously, automatically sterilizing and disinfecting the culture dish and the dish cover before split charging.
The combination of the high-pressure air pump and the electric heating wire is mainly used for generating high-pressure high-temperature gas which acts on the lifted culture dish, so that the culture dish can be sterilized conveniently. And in the process that the culture dish is packaged with the culture medium and falls down, the dish cover is sterilized by means of high-pressure high-temperature gas, and meanwhile, the dish cover is abutted to the culture dish by means of air pressure to assist the falling down of the dish cover.
Other features and advantages of the present invention will be disclosed in the following detailed description of the invention and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of an automated immunocyte medium dispensing apparatus according to the present invention.
FIG. 2 is a block diagram of a culture medium feeding mechanism and a capping mechanism of the automated immune cell culture medium dispensing device of FIG. 1.
FIG. 3 is a top view cross-sectional schematic diagram of a mating connection of a vertical cartridge and a plunger syringe of the automated immunocyte medium dispensing apparatus of FIG. 2.
Fig. 4 is an enlarged structural view at a in fig. 1.
Fig. 5 is a right-side view block diagram showing relative position distribution of a lifting column, a first trigger button switch and a first elastic rubber sheet of the automated immunocyte medium split charging device in fig. 4.
Fig. 6 is a top view of the automated immune cell culture medium dispensing device of fig. 1 in a configuration in which a turntable mechanism is cooperatively coupled with a recovery and boxing mechanism.
FIG. 7 is a right side view of the automated immunocyte medium split charging device of FIG. 6, showing the rotary table mechanism and the recovery and boxing mechanism cooperatively connected.
FIG. 8 is a block diagram of a lifting column of the automated immunocyte medium dispensing apparatus of FIG. 7.
List of reference numerals: an automated immunocyte medium dispensing apparatus 100; a support base plate 10; a main stepping motor 20; a turntable mechanism 30; a retainer ring 31; a backing ring 32; a turntable body 33; a connecting sleeve 34; a lifting column 35; a connecting spring 36; a culture dish 40; a dish cover 50; a conductive cylinder 60; a connecting rope 61; a gravity block 62; a medium feeding mechanism 70; a vertical cylinder 71; a first electric telescopic rod 72; a first trigger key switch 721; a first elastic rubber sheet 722; a docking hole 73; a pressure-sensitive switch 74; a linkage mechanism 75; a linkage ring 751; a link lever 752; a connecting rail 753; a sub stepping motor 754; a gear 755; a rack 756; a piston syringe 76; a one-way valve 761; a piston push rod 77; a first countdown activation switch 78; a capping mechanism 79; a placement box 791; a driving wheel 792; a conveyor belt 793; a second countdown start switch 794; a removable top cover 795; a high temperature air flow sterilization mechanism 80; a high-pressure air pump 81; heating wire 82; a recovery boxing mechanism 90; a second electric telescopic rod 91; an electromagnet 92; a recovery cylinder 93; a pallet 931; a support spring 932; a second elastic rubber sheet 94; a shrink start switch 95; a push rod 96; a second trigger key switch 97; a slide bar 98; a return spring 99.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1 to 8, in an embodiment of the present invention, an automated immune cell culture medium dispensing apparatus 100 includes: a support base plate 10, a main stepper motor 20, a turntable mechanism 30, a culture dish 40 and a dish cover 50. The main stepping motor 20 is fixedly connected to the middle position of the upper end surface of the support base plate 10. The turntable mechanism 30 comprises a limiting ring 31, a supporting ring 32 and a turntable body 33. The turntable body 33 is fixedly connected to a main shaft end of the main stepping motor 20. The carrier rings 32 are equidistantly arranged around the periphery of the turntable body 33. The retainer ring 31 is provided on the upper side of the carrier ring 32. The outer diameter of the culture dish 40 is equal to the inner diameter of the limiting ring 31, the limiting ring 31 is convenient for clamping the culture dish 40, and the supporting ring 32 is used for supporting the bottom of the culture dish 40.
The automated immune cell culture medium dispensing device 100 further comprises: a culture medium feeding mechanism 70 and a transfer drum 60 for vertically stacking the culture dishes 40. The conductive cylinder 60 is vertically disposed at the upper left side of the turntable body 33, and the lower end of the conductive cylinder 60 is attached to the upper end surface of the turntable body 33, while the conductive cylinder 60 is fixedly connected with the support base plate 10.
The medium feeding mechanism 70 includes: a vertical cylinder 71, a first electric telescopic rod 72, a butt joint hole 73, a pressure sensing switch 74, a linkage mechanism 75, a piston injection cylinder 76, a piston push rod 77 and a first countdown start switch 78. The vertical cylinder 71 is vertically provided on the upper side of the right end of the turntable body 33, and the vertical cylinder 71 is fixedly connected with the support base plate 10. The first electric telescopic rod 72 is vertically fixedly connected to the upper side of the right end of the support base plate 10, and the first electric telescopic rod 72 exists in up-down alignment with the vertical cylinder 71. The docking hole 73 is provided at a bottom intermediate position of the culture dish 40. The pressure sensing switch 74 is mounted to the inner wall of the vertical cylinder 71 near the upper end. The piston injection cylinder 76 is fixedly connected horizontally to the upper end outer wall of the vertical cylinder 71, and the injection end of the piston injection cylinder 76 is located inside the vertical cylinder 71. A plunger rod 77 is slidably coupled to the inside of the plunger barrel 76. The piston injection cylinder 76 is equidistantly distributed around the outside of the vertical cylinder 71, and the inner diameters of the piston injection cylinders 76 at different positions are different in size, where the inner diameter of the piston injection cylinder 76 depends on the amount of liquid material to be ejected from the piston injection cylinder 76 when the piston push rod 77 moves by a unit distance. The piston syringes 76 at different positions are filled with the corresponding liquid materials required for the culture medium.
The linkage mechanism 75 includes: linkage ring 751, linkage rod 752, connecting rail 753, secondary stepper motor 754, gear 755, and rack 756. The linkage ring 751 is nested outside the vertical cylinder 71. The link lever 752 is hinged to the outer end of the piston push rod 77, and the end of the link lever 752 is hinged to the link ring 751. The connection rail 753 is vertically fixedly connected to the left outer wall of the vertical cylinder 71. The secondary stepper motor 754 is slidably coupled to the connection rail 753 and the pressure sensitive switch 74 is electrically coupled to the activation circuit of the secondary stepper motor 754. The secondary stepper motor 754 is fixedly coupled to the linkage ring 751. Gear 755 is fixedly coupled to the main shaft end of secondary stepper motor 754. Rack 756 is fixedly attached vertically to the upper end surface of turntable body 33. Gear 755 is engaged with rack 756. A first countdown start switch 78 is mounted to the inner wall of the vertical cylinder 71 and the first countdown start switch 78 is flush with the pressure sensing switch 74. The first countdown start switch 78 is electrically connected to the retraction control circuit of the first electric extension pole 72.
Several empty culture dishes 40 are stacked vertically into the conductive cylinder 60. Each time the main stepping motor 20 drives the rotary table 33 to rotate, the limiting ring 31 on the rotary table 33 just rotates to the lower side of the conductive cylinder 60. The bottom-most dish 40 of the conductive cylinder 60 is then inserted down into the stop collar 31 and supported by the support collar 32. When the limiting ring 31 with the culture dish 40 rotates to the lower side of the vertical cylinder 71 along with the rotary plate body 33, the first electric telescopic rod 72 is started to extend upwards, in the process, the telescopic end of the first electric telescopic rod 72 is inserted into the butt joint hole 73 at the bottom of the culture dish 40, and then the culture dish 40 is jacked upwards into the vertical cylinder 40. When the culture dish 40 is lifted to the position where the pressure sensitive switch 74 is located, the pressure sensitive switch 74 activates the secondary stepper motor 754. The secondary stepper motor 7541 then moves down a distance along the fixed rack 756 by means of the gear 755. The process linkage ring 751 descends along with the process, and all linkage rods 752 are pulled to retract towards the middle, so that piston push rods 77 at all positions are pushed to slide into a section towards the inner side of the piston injection cylinder 76, and the culture medium raw materials in the piston injection cylinder 76 at all positions are extruded and fall into the culture dish 40, so that automatic injection of the culture medium is realized. When the culture dish 40 activates the pressure sensing switch 74, the culture dish 40 also activates the first countdown start switch 78, and the first countdown start switch 78 causes the first electric telescopic rod 72 to retract after a set time. And the first motorized telescopic rod 72 is retracted, the injection of culture medium is just completed, thereby facilitating the return of the culture dish 40 into the stop collar 31.
The conductive cylinder 60 includes: a connecting rope 61 and a weight 62. The gravity pressing block 62 is connected to the upper side of the conductive cylinder 60 by a connection rope 61. When empty dishes 40 are vertically stacked on the inside of the conductive cylinder 60, the gravity pressing block 62 is placed on the uppermost dish 40. So that the whole culture dish 40 is subjected to the gravity of pressing down, and the condition that the culture dish 40 is blocked in the conduction cylinder 60 and cannot slide down is avoided.
A check valve 761 for adding the medium ingredients is installed on the outer wall of the piston syringe 76.
The medium feeding mechanism 70 further includes: a capping mechanism 79. The capping mechanism 79 includes: a holding box 791, a driving wheel 792, a conveying belt 793, a second countdown starting switch 794 and a detachable top cover 795. The placing box 791 is horizontally connected to the right outer wall of the vertical cylinder 71, and the placing box 791 communicates with the inside of the vertical cylinder 71. The driving wheels 792 are arranged at the inner sides of the left end and the right end of the placement box 791 in pairs, and a stepping driving motor is arranged at the driving wheel 792. The belt 793 is disposed circumferentially between the drive wheels 792. The dish covers 50 are horizontally placed on the conveyor belt 793 at equal intervals, and the dish covers 50 are attached to the front and rear inner walls of the placement box 791. A detachable top cover 795 is provided on the upper side of the setting box 791. A second countdown start switch 794 is mounted to the inner wall of the vertical barrel 71 and the second countdown start switch 794 and the first countdown start switch 78 are flush. Meanwhile, the second countdown starting switch 794 is electrically connected with the stepping driving motor arranged on the driving wheel 792, and the countdown time set by the second countdown starting switch 794 is longer than the countdown time of the first countdown starting switch 78, namely, when the first countdown starting switch 78 firstly enables the first electric telescopic rod 72 to shrink to drive the culture dish 40 to descend to be close to the bottom of the vertical cylinder 71, the stepping driving motor on the driving wheel 792 is started by the second countdown starting switch 78. The conveyor 793 is caused to run counter-clockwise a distance to push the leftmost dish cover 50 to traverse into the vertical cylinder 71, and the dish cover 50 then drops onto the culture dish 40 for capping.
The upper port of the culture dish 40 is provided with a magnet ring. The lower edge of the dish cover 50 is provided with an iron ring matched with the magnet ring. The convenient dish cover 50 is attached and sealed on the culture dish 40 by means of magnetism between the magnet rings.
The diameter of the dish cover 50 is equal to the inner diameter of the vertical cylinder 71, and meanwhile, the thickness of the dish cover 50 is equal to the vertical width of a port at the communication position of the placing box 791 and the vertical cylinder 71, so that the dish cover 50 is prevented from tilting when completely traversing to the inner side of the vertical cylinder 71, and the sealing cover is prevented from being influenced.
The turntable mechanism 30 further includes: a connecting sleeve 34, a lifting column 35 and a connecting spring 36. A connection sleeve 34 is connected to the underside of the carrier ring 32. The lifting column 35 is vertically slidably connected to the inner side of the connecting sleeve 34. The connecting spring 36 is sleeved at the lifting column 35, and two ends of the connecting spring 36 are fixedly connected to the lower end of the lifting column 35 and the connecting sleeve 34 respectively. The first electric telescopic link 72 includes: a first trigger button 721 and a first elastic rubber sheet 722. A first elastic rubber piece 722 is attached to the outer wall of the fixed end of the first electric telescopic rod 72. The first trigger button switch 721 is mounted to the first elastic rubber sheet 722. When the culture dish 40 is put into the limiting ring 31, the lifting column 35 slides down along the connecting sleeve 34 due to the gravity of the culture dish 40. At this time, the lower end of the lifting column 35 is at a level with the first trigger key 721. When the rotary table 33 drives the culture dish 40 to rotate to the lower side of the vertical cylinder 71, the lower end of the lifting column 35 just abuts against the first trigger button switch 721 to trigger the first electric telescopic rod 72 to extend. And as the turntable body 33 continues to rotate, the lower end of the lifting column 35 is pressed through the first elastic rubber sheet 722. If there is no culture dish 40 in the limiting ring 31, the lower end of the lifting column 35 is higher than the first trigger button switch 721, so as to avoid triggering the first electric telescopic rod 72.
The upper end of the lifting column 35 is provided with an elastic rubber body, so that the lifting column is convenient to extrude and bend.
The automated immune cell culture medium dispensing device 100 further comprises: a high temperature air flow sterilization mechanism 80. The high temperature air flow sterilization mechanism 80 includes a high pressure air pump 81 and an electric heating wire 82. The high-pressure air pump 81 is mounted to the upper end of the vertical cylinder 71, and the air outlet end of the high-pressure air pump 81 is connected vertically downward to the inside of the upper end of the vertical cylinder 71. The heating wire 82 is installed inside the air outlet end of the high-pressure air pump 81. The heating wire 82 and the high-pressure air pump 81 are electrically connected to a first trigger key switch 721. When the first electric telescopic rod 72 drives the culture dish 40 to lift upwards. The high-pressure air pump 81 generates a strong air flow downward, and the strong air flow passes through the heating wire 82. The heating wire 82 is energized to generate heat so that the strong pressure air flow becomes high temperature air. The high temperature and high pressure air flow then impinges downwardly onto the raised petri dish 40, in alignment for sterilization. And when the dish cover 50 is discharged into the vertical cylinder 71 from the placement box 791, the high-temperature and high-pressure air flow generated by the high-pressure air pump 81 also impinges on the dish cover 50 to sterilize it and assist in rapid falling and docking onto the culture dish 40.
The automated immune cell culture medium dispensing device 100 further comprises: and a recycling and boxing mechanism 90. The recovery boxing mechanism 90 includes: a second electric telescopic rod 91, an electromagnet 92, a recovery cylinder 93, a second elastic rubber sheet 94, a shrinkage start switch 95, a push rod 96, a second trigger button switch 97, a slide rod 98 and a return spring 99. A second electric telescopic rod 91 is fixedly connected horizontally above the front end of the support base plate 10. Electromagnet 92 is fixedly connected to the telescopic end of second electric telescopic rod 91. The recovery cylinder 93 is placed on the upper side of the front end of the support base plate 10. The second elastic rubber piece 94 is provided below the front side edge of the turntable body 33, and the second elastic rubber piece 94 is fixedly connected to the fixed end outer wall of the second electric telescopic rod 91 through a rod body. A contraction start switch 95 is mounted to the second elastic rubber sheet 94, and the contraction start switch 95 is electrically connected to the contraction control circuit of the second electric telescopic rod 91 and the electromagnet 92. The retainer ring 31 is an iron ring. The slide bar 98 is fixedly connected to the stopper ring 31 horizontally, and the slide bar 98 is inserted inside the turntable body 33 horizontally in a sliding manner. A return spring 99 is connected between the slide bar 98 and the turntable body 33. The push rod 96 is fixedly connected to the electromagnet 92 horizontally. The second trigger key switch 97 is mounted to the fixed end of the second electric telescopic rod 91, and the second trigger key switch 97 is electrically connected to the extension control circuit of the second electric telescopic rod 91. The second trigger button switch 97 is present in alignment with the push rod 96. When the turntable 33 drives the capped culture dish 40 to rotate to the direction of the second electric telescopic rod 91, the lifting column 35 protruding downwards is abutted against the contraction starting switch 95, the contraction starting switch 95 causes the electromagnet 92 to be electrified to generate magnetism, and the electromagnet 92 adsorbs the iron limiting ring 31. Simultaneously, the second electric telescopic rod 91 is contracted, and the electromagnet 92 pulls the limiting ring 31 to transversely move to the upper side of the recovery cylinder 93. In this process, the limiting ring 31 is separated from the supporting ring 32, i.e. the lower side of the culture dish 40 is not supported, and the culture dish 40 directly falls into the recovery cylinder 93. And after the second electric telescopic rod 91 is contracted, the ejector rod 96 on the electromagnet 92 is abutted against the second trigger button switch 97, and the second trigger button switch 97 enables the second electric telescopic rod 91 to extend, and the limiting ring 31 is pushed to slide back and reset by means of the electromagnet 92. Then the main stepping motor 20 is started again to drive the turntable body 33 to rotate, and the turntable body 33 overcomes the magnetic force of the electromagnet 92 to rotate, so that the culture dish 40 filled with the culture medium is repeatedly filled.
The recovery cylinder 93 includes: a support plate 931 and a support spring 932. The supporting plate 931 is horizontally disposed inside the recovery cylinder 93. The supporting spring 932 is vertically fixedly connected between the supporting plate 931 and the inner bottom portion of the recovery cylinder 93. When the culture dish 40 falls inside the recovery cylinder 93, it is supported by the support plate 931 and the impact force of the fall of the culture dish 40 is buffered by the resilience of the support spring 932.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (5)
1. An automated immunocyte medium dispensing apparatus, comprising: the culture dish comprises a supporting bottom plate, a main stepping motor, a turntable mechanism, a culture dish and a dish cover; the main stepping motor is fixedly connected to the supporting bottom plate; the turntable mechanism comprises a limiting ring, a supporting ring and a turntable body; the turntable body is fixedly connected to the main shaft end of the main stepping motor; the supporting rings are arranged at the edge of the turntable body in an encircling and equidistant manner; the limiting ring is arranged on the upper side of the supporting ring; it is characterized in that the method comprises the steps of,
the automatic immune cell culture medium split charging device further comprises: the culture medium throwing mechanism and the transmission cylinder are used for vertically stacking the culture dishes; the conduction cylinder is vertically arranged on the left upper side of the turntable body, and is fixedly connected with the supporting bottom plate;
the culture medium throwing mechanism comprises: the device comprises a vertical cylinder, a first electric telescopic rod, a butt joint hole, a pressure sensing switch, a linkage mechanism, a piston injection cylinder, a piston push rod and a first countdown starting switch; the vertical cylinder is vertically arranged on the upper side of the right end of the turntable body and is fixedly connected with the supporting bottom plate; the first electric telescopic rod is vertically and fixedly connected to the upper side of the right end of the supporting bottom plate; the docking Kong Kaishe is at the bottom of the culture dish; the pressure sensing switch is mounted on the inner wall of the vertical cylinder; the piston injection cylinder is horizontally and fixedly connected to the outer wall of the upper end of the vertical cylinder; the piston push rod is connected to the inner side of the piston injection cylinder in a sliding fit manner;
the linkage mechanism comprises: the device comprises a linkage ring, a linkage rod, a connecting guide rail, an auxiliary stepping motor, a gear and a rack; the linkage ring is sleeved outside the vertical cylinder; the linkage rod is movably connected to the outer end of the piston push rod through a hinge, and the tail end of the linkage rod is movably connected to the linkage ring through a hinge; the connecting guide rail is vertically and fixedly connected to the outer wall of the vertical cylinder; the auxiliary stepping motor is connected to the connecting guide rail in a sliding manner, and the pressure sensing switch is electrically connected with a starting circuit of the auxiliary stepping motor; the auxiliary stepping motor is fixedly connected with the linkage ring; the gear is fixedly connected to the main shaft end of the auxiliary stepping motor; the rack is vertically and fixedly connected to the turntable body; the gear is meshed with the rack; the first countdown starting switch is mounted on the inner wall of the vertical cylinder; the first countdown starting switch is electrically connected with a contraction control circuit of the first electric telescopic rod;
the conductive cylinder includes: the connecting rope and the gravity pressing block are connected; the gravity pressing block is connected to the upper side of the conduction cylinder through the connecting rope;
the culture medium throwing mechanism further comprises: a capping mechanism; the capping mechanism includes: the device comprises a placing box, a driving wheel, a conveying belt, a second countdown starting switch and a detachable top cover; the placing box is horizontally connected to the outer wall of the vertical cylinder, and is communicated with the inside of the vertical cylinder; the driving wheels are arranged on the inner side of the placement box in pairs left and right, and a stepping driving motor is arranged at the driving wheels; the conveyor belt is arranged between the driving wheels in a surrounding way; the dish covers are transversely and equidistantly arranged on the conveyor belt; the detachable top cover is arranged on the upper side of the placement box; the second countdown starting switch is mounted on the inner wall of the vertical cylinder; simultaneously, the second countdown starting switch is electrically connected with the stepping driving motor arranged on the driving wheel;
the turntable mechanism further includes: the lifting column is connected with the lifting column through a connecting sleeve; the connecting sleeve is connected to the lower side of the supporting ring; the lifting column is vertically connected to the inner side of the connecting sleeve in a sliding manner; the connecting spring is sleeved at the lifting column, and two ends of the connecting spring are fixedly connected to the lower end of the lifting column and the connecting sleeve respectively; the first electric telescopic rod includes: the first trigger button switch and the first elastic rubber sheet; the first elastic rubber sheet is connected to the outer wall of the fixed end of the first electric telescopic rod; the first trigger button switch is arranged at the first elastic rubber sheet;
the upper end of the lifting column is provided with an elastic rubber body;
the automatic immune cell culture medium split charging device further comprises: a high temperature air flow sterilization mechanism; the high-temperature airflow sterilization mechanism comprises a high-pressure air pump and an electric heating wire; the high-pressure air pump is mounted to the upper end of the vertical cylinder, and the air outlet end of the high-pressure air pump is vertically downwards connected to the inner side of the vertical cylinder; the heating wire is arranged on the inner side of the air outlet end of the high-pressure air pump; the heating wire and the high-pressure air pump are electrically connected with the first trigger button switch.
2. An automated immunocyte medium dispensing apparatus as described in claim 1, wherein,
the outer wall of the piston injection cylinder is provided with a one-way valve for adding culture medium ingredients.
3. An automated immunocyte medium dispensing apparatus as described in claim 1, wherein,
a magnet ring is arranged at the upper port of the culture dish; the lower end edge of the dish cover is provided with an iron ring matched with the magnet ring.
4. An automated immunocyte medium dispensing apparatus as described in claim 1, wherein,
the automatic immune cell culture medium split charging device further comprises: a recycling and boxing mechanism; the recycling and boxing mechanism comprises: the second electric telescopic rod, the electromagnet, the recovery cylinder, the second elastic rubber sheet, the shrinkage start switch, the ejector rod, the second trigger button switch, the sliding rod and the reset spring; the second electric telescopic rod is horizontally and fixedly connected to the upper part of the front end of the supporting bottom plate; the electromagnet is fixedly connected to the telescopic end of the second electric telescopic rod; the recovery cylinder is arranged on the upper side of the front end of the supporting bottom plate; the second elastic rubber sheet is arranged below the front side edge of the turntable body and is fixedly connected to the outer wall of the fixed end of the second electric telescopic rod through a rod body; the contraction starting switch is mounted at the second elastic rubber sheet and is connected with a contraction control circuit of the second electric telescopic rod and the electromagnetic iron; the limiting ring is an iron ring; the sliding rod is horizontally and fixedly connected to the limiting ring, and horizontally slides and penetrates through the inner side of the turntable body; the reset spring is connected between the sliding rod and the turntable body; the ejector rod is horizontally and fixedly connected to the electromagnet; the second trigger button switch is mounted to the fixed end of the second electric telescopic rod, and is electrically connected with the extension control circuit of the second electric telescopic rod.
5. The automated immunocyte medium dispensing apparatus of claim 4, wherein,
the recovery cylinder includes: a pallet and a support spring; the supporting plate is horizontally arranged on the inner side of the recovery cylinder; the support spring is vertically and fixedly connected between the supporting plate and the inner bottom of the recovery cylinder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110927218.1A CN113604350B (en) | 2021-08-13 | 2021-08-13 | Automatic change immune cell culture medium partial shipment device |
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| CN202110927218.1A CN113604350B (en) | 2021-08-13 | 2021-08-13 | Automatic change immune cell culture medium partial shipment device |
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| CN202440494U (en) * | 2012-03-06 | 2012-09-19 | 牛刚 | Preparation device of minitype sterile precast culture dish |
| CN107083318A (en) * | 2017-04-10 | 2017-08-22 | 杭州吉沃科技有限公司 | A kind of culture medium auto-loading system |
| CN112961756A (en) * | 2020-12-22 | 2021-06-15 | 杭州中赢生物医疗科技有限公司 | Liquid adding device based on biological experiment equipment and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| FR2948945B1 (en) * | 2009-08-07 | 2012-03-09 | Aes Chemunex | DEVICE AND METHOD FOR DISPENSING A PRODUCT IN A PETRI BOX |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202440494U (en) * | 2012-03-06 | 2012-09-19 | 牛刚 | Preparation device of minitype sterile precast culture dish |
| CN107083318A (en) * | 2017-04-10 | 2017-08-22 | 杭州吉沃科技有限公司 | A kind of culture medium auto-loading system |
| CN112961756A (en) * | 2020-12-22 | 2021-06-15 | 杭州中赢生物医疗科技有限公司 | Liquid adding device based on biological experiment equipment and use method thereof |
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