CN113603380A - Method for reinforcing recycled concrete - Google Patents
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- CN113603380A CN113603380A CN202111008357.0A CN202111008357A CN113603380A CN 113603380 A CN113603380 A CN 113603380A CN 202111008357 A CN202111008357 A CN 202111008357A CN 113603380 A CN113603380 A CN 113603380A
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Abstract
The invention discloses a method for reinforcing recycled concrete, which belongs to the technical field of biology and comprises the following steps: preparing a culture medium; inoculating and culturing: inoculating the bacillus pasteurii seed liquid into a culture medium, and culturing at constant temperature of 30 ℃ and at the stirring speed of 180 rpm; introducing compressed air for aerobic culture to obtain a bacillus pasteurii culture solution; mineralization and strengthening: soaking the concrete regenerated particles in diluted pasteuria bacillus culture solution for 1-3h, and then carrying out mineralization treatment, wherein in the mineralization process, the pasteuria bacillus culture solution is circulated through a feed liquid pump, and urea and calcium nitrate are added simultaneously, so that a mineralized solution containing 0.6-0.9mol/L of urea and calcium nitrate is obtained, and the temperature of the mineralized solution is 25-30 ℃; drying: and drying the mineralized solution to obtain regenerated mineralized particles with the water content of 8-9%. The invention has the advantages of continuous culture, simple process and better strengthening effect.
Description
Technical Field
The invention relates to the field of biotechnology, in particular to a method for reinforcing recycled concrete.
Background
In 2018, the total production of cement is nearly 40 hundred million tons. The production process of portland cement (also known as portland cement abroad) consumes a large amount of energy and resources, and discharges a large amount of dust and exhaust gas, which has a serious impact on the ecological environment. Therefore, the development of energy-saving, environment-friendly and durable green cement materials is urgently needed. Biological cement is formed by mineral gelation induced by microorganisms, and has become a research hotspot in various fields at home and abroad due to the advantages of environmental friendliness and the like. Among them the most well known and extensively studied contemporary microorganisms induce calcium carbonate mineralization MICP. In the microbial mineralization of calcium carbonate, however, the model of hydrolysis of urea by urease to produce mineralized products is the simplest and most studied model and is often used in the application of MICP. By virtue of its high urease activity, pasteuria has become one of the most popular bacteria in MICP-related research.
Paecilomyces pasteurianus belongs to the genus Sporosarcina (also known as Sporosarcina pasteurianus) of the family Mycosphaerellales, and is a non-pathogenic gram-positive bacterium isolated from soil. The Papanicolaou bacillus cell is rod-shaped, has the length of about 2-3 mu m, has strong viability, can grow at 15-37 ℃, can continuously survive in the form of spores when lacking nutrient substances, and has round spores with the diameter of about 0.5-1.5 mu m. The spore-forming property of the pasteurella becomes one of the core elements for many scholars to develop the pasteurella into self-repairing concrete (cement).
At present, the case of applying the pasteurella to the recycled concrete appears in the market, but the problems that the pasteurella culture process is complicated, the continuous culture cannot be carried out, the reinforcing effect of the recycled concrete is poor and the like generally exist.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a method for reinforcing recycled concrete, which has the advantages of continuous culture, simple process and better reinforcing effect.
In order to achieve the purpose, the invention provides the following technical scheme:
a method of reinforcing recycled concrete comprising the steps of:
s1 preparation of culture medium
The culture medium consists of the following components and concentrations thereof: 5-10g/L of yeast extract, 5-15g/L of soybean peptone, 10-30g/L of glucose, 3-8g/L of ammonium sulfate, 10-15mg/L of magnesium sulfate monohydrate and 15-20mg/L of nickel chloride hexahydrate; weighing the above components, adjusting pH to 8.0 with 0.5mol/L sodium hydroxide solution, preparing 2.5L culture medium, placing in 5L fermentation tank, heating to 120 deg.C for sterilization, maintaining the temperature for 15min, and cooling to 30 deg.C to obtain culture medium;
s2, inoculation and culture
Inoculating the Bacillus pasteurii seed liquid into the culture medium according to the inoculation amount of 0.5-2.0%, and culturing at constant temperature of 30 ℃ and the stirring speed of 180rpm for 3-7 days; introducing compressed air for aerobic culture, wherein the aeration ratio is 1:0.5(V/V.min) at the early stage of culture, when the relative dissolved oxygen is less than 50%, the aeration ratio is increased to more than 1:1(V/V.min), and continuously feeding a glucose solution with the mass concentration of 25% as a supplementing material; adjusting the flow rate of the compressed air to keep more than 30% of the relative dissolved oxygen, stopping feeding when the relative dissolved oxygen continuously rises, feeding ammonia water in the whole culture process to keep the pH value constant at 8.0, and obtaining a pasteurella culture solution after the culture is finished; diluting the Paenibacillus pasteurianus culture solution to OD600 of 0.6-1.2 for later use;
s3 mineralization enhancement
Soaking the concrete regenerated particles in the pasteuria bacillus culture solution diluted in the step S2 for 1-3h, and then carrying out mineralization treatment, wherein in the mineralization process, the pasteuria bacillus culture solution is circulated through a feed liquid pump, and urea and calcium nitrate are added simultaneously, so that a mineralized solution containing 0.6-0.9mol/L of urea and calcium nitrate is obtained, and the temperature of the mineralized solution is 25-30 ℃;
s4, drying
And (5) drying the mineralization solution obtained in the step (S3) to obtain the regenerated mineralization particles with the water content of 8-9%.
More preferably: in step S2, the glucose solution is fed with a supplement in an amount of 50-100ml/2.5L per hour, and the supplement is fed for 6-10 h.
More preferably: in step S1, the amount of the medium charged is 50%.
More preferably: in step S1, the fermentation tank includes a tank body, a magnetic stirring device, a sterilizing lamp, a driving mechanism, and a tank door disposed on one side of the tank body, the tank body is provided with a steam pipe and an exhaust valve, the steam pipe is inserted into the tank body from top to bottom, an air outlet is disposed on the circumferential surface of the steam pipe, and the magnetic stirring device is mounted at the bottom of the tank body;
the sterilizing lamps comprise a first sterilizing lamp and a second sterilizing lamp, the first sterilizing lamp and the second sterilizing lamp are respectively positioned between the steam pipe and the side wall of the tank body, and the driving mechanism is used for driving the first sterilizing lamp and the second sterilizing lamp to be close to or far away from each other.
More preferably: the driving mechanism comprises a guide rod, a fixed plate, a moving platform, a movable disc, a connecting rod and a cylinder;
the movable tables comprise a first movable table and a second movable table, the first sterilizing lamp is installed on the first movable table, the second sterilizing lamp is installed on the second movable table, the guide rod is vertically arranged, two ends of the guide rod are respectively fixed on the side wall of the tank body, and the first movable table and the second movable table are respectively in sliding fit with the guide rod;
the fixed plate is fixed in the middle of the guide rod, the first mobile station and the second mobile station are respectively located on two opposite sides of the fixed plate, a rotating shaft is arranged on one side, close to the steam pipe, of the fixed plate, one rotating end of the fixed plate is fixed on the fixed plate, the other rotating end of the fixed plate penetrates through the middle of the movable disc, the connecting rod comprises a first connecting rod and a second connecting rod, one end of the first connecting rod is rotatably connected with the first mobile station, the other end of the first connecting rod is rotatably connected with the movable disc, one end of the second connecting rod is rotatably connected with the second mobile station, the other end of the second connecting rod is rotatably connected with the movable disc, and the first connecting rod and the second connecting rod are respectively connected with two ends of the movable disc;
the cylinder is fixed on the top of the tank body and comprises a piston rod, and the piston rod of the cylinder is fixed with the fixing plate.
More preferably: the first sterilizing lamp and the second sterilizing lamp are respectively provided with two sterilizing lamps, and the two sterilizing lamps are respectively positioned on two opposite sides of the steam pipe.
More preferably: the steam pipe is characterized in that the plurality of air outlet holes are uniformly distributed on the surface of the steam pipe.
More preferably: the overlooking projection of the tank body is circular, and the steam pipe is vertically arranged and inserted into the bottom of the tank body.
In conclusion, the invention has the following beneficial effects: the magnesium ions which are beneficial to the absorption of the culture are added into the culture medium provided by the invention, so that the absorption of the metal ions by the bacillus pasteurianus is facilitated, and the effect of promoting the growth is achieved. The soybean peptone and the glucose are added into the culture medium provided by the invention, which is helpful for the rapid absorption of saccharides, proteins and vitamins, thereby promoting the rapid propagation and growth of the bacillus pasteurii. The high-density culture method of the pasteurella provided by the invention can obtain the pasteurella with the spore formation rate of more than 95 percent and the lowest spore density of 1.5 multiplied by 1010cfu/ml, good continuity, simple process and convenient operation, and is convenient for large-scale culture. The bacillus pasteurii can secrete high-activity urease, and the high-efficiency urease activity is utilized to catalyze the urea decomposition in the bacteria, so that the carbonate concentration and the pH value of a microenvironment around the bacteria are rapidly increased; secondly, the surface of B.pasteurii (including cell walls and extracellular polymeric substances) has more surface than that of non-mineralized bacteriaA negative surface charge, a stronger adsorption of cations, and thus Ca with a positive charge in the environment2+Can be adsorbed on the surface of the Pasteurella multocida in a large amount, and Ca can be generated under the conditions of high concentration of carbonate and alkalinity2+Will settle to form CaCO by using cells as crystal nucleus3And crystallization, so that the bacillus pasteurii can induce calcium carbonate precipitation to strengthen mineralized concrete regenerated particles (fine aggregates and the like).
Drawings
FIG. 1 is a block flow diagram of an example, which is mainly used for embodying a high-density culture method of Bacillus pasteurii;
FIG. 2 is a schematic structural view of an embodiment, which is mainly used for embodying the internal structure of a fermentation tank;
fig. 3 is a schematic structural diagram of the embodiment, which is mainly used for embodying the structure of the driving mechanism.
In the figure, 1, a tank body; 2. an air outlet; 3. a magnetic stirring device; 4. a support leg; 5. a steam pipe; 6. an exhaust valve; 71. a first germicidal lamp; 72. a second germicidal lamp; 81. a guide bar; 82. a fixing plate; 83. a mobile station; 831. a first mobile station; 832. a second mobile station; 84. a movable tray; 85. a connecting rod; 851. a first link; 852. a second link; 86. a cylinder; 9. and (4) air is introduced into the pipe.
Detailed Description
The invention is described in detail below with reference to the figures and examples.
Example 1: a method for reinforcing recycled concrete, as shown in fig. 1, comprising the steps of:
s1 preparation of culture medium
The culture medium consists of the following components and concentrations thereof: 8g/L of yeast extract, 10g/L of soybean peptone, 18g/L of glucose, 5g/L of ammonium sulfate, 12mg/L of magnesium sulfate monohydrate and 18mg/L of nickel chloride hexahydrate; weighing the above components, adjusting pH to 8.0 with 0.5mol/L sodium hydroxide solution, preparing 2.5L culture medium, placing in 5L fermentation tank, heating to 120 deg.C for sterilization, maintaining the temperature for 15min, and cooling to room temperature to obtain culture medium; preferably, the medium is filled to 50%, i.e.to 2500ml at 5000ml volume.
S2, inoculation and culture
Under the aseptic operation condition, inoculating the bacillus pasteurii seed liquid into a culture medium according to the inoculation amount of 1%, and culturing for 4 days at constant temperature of 30 ℃ and at the stirring speed of 180 rpm; introducing compressed air for aerobic culture, wherein the aeration ratio is 1:0.5(V/V.min) at the early stage of culture, when the relative dissolved oxygen is lower than 50%, the aeration ratio is increased to more than 1:1(V/V.min), continuous feeding of glucose solution with the mass concentration of 25% is started, the feeding amount per hour is 80ml/2.5L of culture medium, and feeding is carried out for 7 h; adjusting compressed air flow to maintain a relative dissolved oxygen content of above 30%, when the relative dissolved oxygen content rises continuously, indicating that the thallus propagation speed is reduced, stopping feeding, feeding ammonia water in the whole culture process to keep constant pH value of 8.0, and after the culture is finished, obtaining a Bacillus pasteurianus culture solution with a Bacillus density of 1.9 × 1010cfu/ml; diluting the pasteurella bacillus culture solution to OD600 of 1.0 for later use;
s3 mineralization enhancement
Soaking the concrete regeneration particles with the particle size of 5-15 mm in the pasteuria bacillus culture solution diluted in the step S2 for 2h, and then carrying out mineralization treatment, wherein in the mineralization process, the pasteuria bacillus culture solution is circulated through a material liquid pump so that the pasteuria bacillus culture solution is circulated under the suction of the material liquid pump, and the fluidity of the pasteuria bacillus culture solution is improved; simultaneously adding urea and calcium nitrate to obtain a mineralized solution containing 0.8mol/L of both urea and calcium nitrate, wherein the temperature of the mineralized solution is kept at 28 ℃; preferably, in the mineralization process, after the concrete regenerated particles in the first mineralization pool are soaked and mineralized for 2 hours, the mineralization solution is emptied into the other mineralization pool to be soaked for 2 hours, and the cycle is repeated for 15 times; preferably, during the mineralization process, the pasteuria bacillus culture solution is introduced into the mineralization pool from the bottom of the mineralization pool and is pumped out from the top through a feed liquid pump.
S4, drying
And (5) drying the mineralization solution obtained in the step (S3) to obtain the regenerated mineralization particles with the water content of 8%.
In the technical scheme, the magnesium ions which are beneficial to the absorption of the culture and are beneficial to the metal ions of the bacillus pasteurianus are added into the culture medium provided by the inventionIt can be absorbed to promote growth. The soybean peptone and the glucose are added into the culture medium provided by the invention, which is helpful for the rapid absorption of saccharides, proteins and vitamins, thereby promoting the rapid propagation and growth of the bacillus pasteurii. The high-density culture method of the pasteurella provided by the invention can obtain the pasteurella with the spore forming rate of more than 95 percent and the lowest density of the bacillus of 1.5 multiplied by 1010cfu/ml, simple process, strong continuity and convenient large-scale culture. The bacillus pasteurii can secrete high-activity urease, and the high-efficiency urease activity is utilized to catalyze the urea decomposition in the bacteria, so that the carbonate concentration and the pH value of a microenvironment around the bacteria are rapidly increased; secondly, the surface of the Pasteurella (including cell walls and extracellular polymeric substances) has more negative surface charge and stronger adsorption to cations than non-mineralized bacteria, so that Ca with positive charge in the environment2+Can be adsorbed on the surface of the Pasteurella multocida in a large amount, and Ca can be generated under the conditions of high concentration of carbonate and alkalinity2+Will settle to form CaCO by using cells as crystal nucleus3And crystallization, so that the bacillus pasteurii can induce calcium carbonate precipitation to strengthen mineralized concrete regenerated particles (fine aggregates and the like).
Referring to fig. 2-3, in step S, the fermenter includes a tank 1, a sterilizing lamp, a magnetic stirring apparatus 3, a driving mechanism, and a door provided at one side of the tank 1. The tank body 1 is cylindrical and has a circular overlook projection, and the door is arranged at one side of the tank body 1 and is externally fixed with a handle so as to open or close the door. The magnetic stirring device 3 is arranged at the center of the bottom of the tank body 1, the bottom of the tank body 1 is fixed with two supporting legs 4, and the two supporting legs 4 are symmetrically arranged on two sides of the bottom of the tank body 1. The steam pipe 5, the exhaust valve 6 and the air inlet pipe 9 for air to enter are arranged on the tank body 1, the air inlet pipe 9 extends into the bottom in the tank body 1, the steam pipe 5 is inserted into the tank body 1 from top to bottom, and the steam pipe 5 is vertically arranged and inserted into the bottom of the tank body 1. The exhaust valve 6 is installed on the top of the can body 1, and preferably, a pressure gauge and a thermometer may be installed on the top of the can body 1 in order to observe the pressure and temperature inside the can body 1. Circular venthole 2 has been seted up on the circumferential surface of steam pipe 5 lower part, and venthole 2 is located jar internal 1, and venthole 2 is provided with a plurality ofly, and a plurality of ventholes 2 equipartitions are on 5 circumferential surface of steam pipe.
In the technical scheme, the fermentation tank adopts two combined sterilization modes of steam sterilization and ultraviolet sterilization, external steam can enter the bottom of the tank body 1 through the steam pipe 5, and the steam entering the tank body 1 is discharged through the air outlet 2. Steam can be rapidly and uniformly contacted with the culture medium after being discharged through the air outlet 2, and the sterilization lamp is matched for use, so that the effects of comprehensive disinfection and high sterilization efficiency can be achieved.
Referring to fig. 2 to 3, the sterilizing lamps include a first sterilizing lamp 71 and a second sterilizing lamp 72, the first sterilizing lamp 71 and the second sterilizing lamp 72 are respectively located between the steam pipe 5 and the sidewall of the can body 1, and both the first sterilizing lamp 71 and the second sterilizing lamp 72 are ultraviolet lamps. The driving mechanism is used for driving the first and second germicidal lamps 71 and 72 to move toward or away from each other, and preferably, the driving mechanism includes a guide rod 81, a fixed plate 82, a moving table 83, a movable disk 84, a connecting rod 85, and a cylinder 86. The mobile station 83 includes a first mobile station 831 and a second mobile station 832, the first germicidal lamp 71 is installed on the side of the first mobile station 831 near the steam pipe 5, and the second germicidal lamp 72 is installed on the side of the second mobile station 832 near the steam pipe 5. The guide rods 81 are vertically arranged, two ends of each guide rod 81 are respectively fixed on the side walls of the upper side and the lower side of the tank body 1, the two guide rods 81 are arranged in parallel, and the two guide rods 81 are respectively symmetrically arranged on two opposite sides of the steam pipe 5. The first mobile station 831 and the second mobile station 832 are respectively slidably engaged with the guide rods 81, and specifically, both of the guide rods 81 pass through the first mobile station 831 and the second mobile station 832, so that the first mobile station 831 and the second mobile station 832 are close to or far away from each other along the length direction of the guide rods 81.
Referring to fig. 2 to 3, the fixing plate 82 is fixed in the middle of the guide rod 81, and the first movable stage 831 and the second movable stage 832 are respectively located at opposite sides of the fixing plate 82, specifically, the first movable stage 831 is located above the second movable stage 832, and the first germicidal lamp 71 is located above the second germicidal lamp 72. The fixed plate 82 is provided with a rotating shaft at a side close to the steam pipe 5, one end of the rotating shaft is fixed on the fixed plate 82, and the other end passes through the middle of the movable plate 84. The link 85 includes a first link 851 and a second link 852, the first link 851 being positioned above the second link 852 and having one end rotatably connected to the first movable stage 831 and the other end rotatably connected to the movable plate 84. The second link 852 is rotatably connected to the second movable stage 832 at one end and to the movable plate 84 at the other end, and the first link 851 and the second link 852 are connected to both ends of the movable plate 84, respectively. The cylinder 86 is fixed above the top of the tank body 1, the cylinder 86 comprises a piston rod, and the end part of the piston rod of the cylinder 86 is fixed with the fixing plate 82. In order to perform the overall sterilization, it is preferable that the first and second sterilizing lamps 71 and 72 are provided in two, and the two first sterilizing lamps 71 and the two second sterilizing lamps 72 are respectively located at opposite sides of the steam pipe 5.
In the above technical solution, during sterilization, the cylinder 86 is started, because the fixed plate 82 is fixed on the guide bar 81, and the first mobile platform 831 and the second mobile platform 832 are both slidably connected on the guide bar 81, therefore when the piston rod of the cylinder 86 contracts back and forth, the first mobile platform 831 and the second mobile platform 832 drive the first sterilizing lamp 71 and the second sterilizing lamp 72 to be close to and away from each other, thereby the first sterilizing lamp 71 and the second sterilizing lamp 72 can move back and forth in the vertical direction, the sterilization uniformity and the comprehensiveness are improved, the sterilization effect is better, and the automation degree is high.
Example 2: a method for reinforcing recycled concrete, which is different from embodiment 1 in that it comprises the steps of:
s1 preparation of culture medium
The culture medium consists of the following components and concentrations thereof: 5g/L of yeast extract, 5g/L of soybean peptone, 10g/L of glucose, 3g/L of ammonium sulfate, 10mg/L of magnesium sulfate monohydrate and 15mg/L of nickel chloride hexahydrate; weighing the above components, adjusting pH to 8.0 with 0.5mol/L sodium hydroxide solution, preparing 2.5L culture medium, placing in 5L fermentation tank, heating to 120 deg.C for sterilization, maintaining the temperature for 15min, and cooling to 30 deg.C to obtain culture medium; preferably, the medium is filled to 50%, i.e.to 2500ml at 5000ml volume.
S2, inoculation and culture
Under the aseptic operation condition, inoculating the bacillus pasteurii seed liquid into a culture medium according to the inoculum size of 0.5%, and culturing for 3 days at the constant temperature of 30 ℃ and the stirring speed of 180 rpm; introducing compressed air for aerobic culture, wherein the aeration ratio is 1:0.5(V/V.min) at the early stage of culture, when the relative dissolved oxygen is lower than 50%, the aeration ratio is increased to more than 1:1(V/V.min), continuous feeding of glucose solution with the mass concentration of 25% is started, the feeding amount is 50ml/2.5L of culture medium per hour, and feeding is carried out for 6 hours in a co-flow manner; adjusting the flow rate of the compressed air to keep more than 30% of the relative dissolved oxygen, when the relative dissolved oxygen continuously rises, indicating that the reproduction speed of the thalli is reduced, stopping feeding, feeding ammonia water in the whole culture process to keep the pH value constant at 8.0, and obtaining a bacillus pasteurii culture solution after the culture is finished; diluting the pasteuria bacillus culture solution to OD600 of 0.6 for later use;
s3 mineralization enhancement
Soaking the concrete regeneration particles with the particle size of 5-15 mm in the pasteuria bacillus culture solution diluted in the step S2 for 1h, and then carrying out mineralization treatment, wherein in the mineralization process, the pasteuria bacillus culture solution is circulated through a material liquid pump so that the pasteuria bacillus culture solution is circulated under the suction of the material liquid pump, and the fluidity of the pasteuria bacillus culture solution is improved; simultaneously adding urea and calcium nitrate to obtain a mineralized solution containing 0.6mol/L of both urea and calcium nitrate, wherein the temperature of the mineralized solution is kept at 25 ℃; preferably, in the mineralization process, after the concrete regenerated particles in the first mineralization pool are soaked and mineralized for 2 hours, the mineralization solution is emptied into the other mineralization pool to be soaked for 2 hours, and the cycle is repeated for 12 times; preferably, during the mineralization process, the pasteuria bacillus culture solution is introduced into the mineralization pool from the bottom of the mineralization pool and is pumped out from the top through a feed liquid pump.
S4, drying
And (5) drying the mineralization solution obtained in the step (S3) to obtain the regenerated mineralization particles with the water content of 9%.
Example 3: a method for reinforcing recycled concrete, which is different from embodiment 1 in that it comprises the steps of:
s1 preparation of culture medium
The culture medium consists of the following components and concentrations thereof: 10g/L of yeast extract, 15g/L of soybean peptone, 30g/L of glucose, 8g/L of ammonium sulfate, 15mg/L of magnesium sulfate monohydrate and 20mg/L of nickel chloride hexahydrate; weighing the above components, adjusting pH to 8.0 with 0.5mol/L sodium hydroxide solution, preparing 2.5L culture medium, placing in 5L fermentation tank, heating to 120 deg.C for sterilization, maintaining the temperature for 15min, and cooling to room temperature to obtain culture medium; preferably, the medium is filled to 50%, i.e.to 2500ml at 5000ml volume.
S2, inoculation and culture
Under the aseptic operation condition, inoculating the bacillus pasteurii seed liquid into a culture medium according to the inoculum size of 2.0%, and culturing for 3 days at the constant temperature of 30 ℃ and the stirring speed of 180 rpm; introducing compressed air for aerobic culture, wherein the aeration ratio is 1:0.5(V/V.min) at the early stage of culture, when the relative dissolved oxygen is lower than 50%, the aeration ratio is increased to more than 1:1(V/V.min), continuous feeding of glucose solution with the mass concentration of 25% is started, the feeding amount is 100ml/2.5L of culture medium per hour, and feeding is carried out for 10 h; adjusting the flow rate of the compressed air to keep more than 30% of the relative dissolved oxygen, when the relative dissolved oxygen continuously rises, indicating that the reproduction speed of the thalli is reduced, stopping feeding, feeding ammonia water in the whole culture process to keep the pH value constant at 8.0, and obtaining a bacillus pasteurii culture solution after the culture is finished; diluting the pasteuria bacillus culture solution to OD600 of 1.2 for later use;
s3 mineralization enhancement
Soaking the concrete regeneration particles with the particle size of 5-15 mm in the pasteuria bacillus culture solution diluted in the step S2 for 3h, and then carrying out mineralization treatment, wherein in the mineralization process, the pasteuria bacillus culture solution is circulated through a material liquid pump so that the pasteuria bacillus culture solution is circulated under the suction of the material liquid pump, and the fluidity of the pasteuria bacillus culture solution is improved; simultaneously adding urea and calcium nitrate to obtain a mineralized solution containing 0.9mol/L of both urea and calcium nitrate, wherein the temperature of the mineralized solution is kept at 30 ℃; preferably, in the mineralization process, after the concrete regenerated particles in the first mineralization pool are soaked and mineralized for 2 hours, the mineralization solution is emptied into the other mineralization pool to be soaked for 2 hours, and the cycle is repeated for 20 times; preferably, during the mineralization process, the pasteuria bacillus culture solution is introduced into the mineralization pool from the bottom of the mineralization pool and is pumped out from the top through a feed liquid pump.
S4, drying
And (5) drying the mineralization solution obtained in the step (S3) to obtain the regenerated mineralization particles with the water content of 9%.
In order to examine the compressive strength of the concrete test blocks made of the recycled concrete particles reinforced in examples 1 to 3, the present invention performed a control test by the following specific method:
blank group
Preparing a concrete test block from the recycled concrete particles without strengthening treatment according to the following formula: 250g of cement 42.5, 80g of mineral powder, 500g of unreinforced recycled concrete particles, 300g of river sand, 100g of broken stone and 140g of water.
The concrete test block is prepared according to the following formula: 250g of cement 42.5, 80g of mineral powder, 500g of recycled reinforced concrete particles in example 1, 300g of river sand, 100g of broken stone and 140g of water.
The concrete test block is prepared according to the following formula: 250g of cement 42.5, 80g of mineral powder, 500g of recycled reinforced concrete particles in example 2, 300g of river sand, 100g of broken stone and 140g of water.
Test 3 groups
The concrete test block is prepared according to the following formula: 250g of cement 42.5, 80g of mineral powder, 500g of recycled reinforced concrete particles in example 3, 300g of river sand, 100g of broken stone and 140g of water.
Preparing 3 cube concrete test blocks with the side length of 150mm for each group of test blocks, and curing under standard conditions for 28d to test the compressive strength. The invention tests the compression strength performance of the concrete test block samples of blank group, test 1 group, test 2 group and test 3 group, the compression strength is carried out according to GB/T50081-2019 'test method standard for physical and mechanical properties of concrete', the average values of the compression strength of 3 samples are respectively 27.0MPa of blank group, 32.3MPa of test 1 group, 31.8MPa of test 2 group and 33.4MPa of test 3 group.
It can be seen that the compressive strength of the concrete test blocks made from the reinforced recycled concrete particles of examples 1 to 3 of the present invention is higher than that of the concrete test blocks made from the unreinforced recycled concrete particles.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that several improvements and modifications without departing from the principle of the present invention will occur to those skilled in the art, and such improvements and modifications should also be construed as within the scope of the present invention.
Claims (9)
1. A method for reinforcing recycled concrete, which is characterized by comprising the following steps: the method comprises the following steps:
s1 preparation of culture medium
The culture medium consists of the following components and concentrations thereof: 5-10g/L of yeast extract, 5-15g/L of soybean peptone, 10-30g/L of glucose, 3-8g/L of ammonium sulfate, 10-15mg/L of magnesium sulfate monohydrate and 15-20mg/L of nickel chloride hexahydrate; weighing the above components, adjusting pH to 8.0 with 0.5mol/L sodium hydroxide solution, preparing 2.5L culture medium, placing in 5L fermentation tank, heating to 120 deg.C for sterilization, maintaining the temperature for 15min, and cooling to 30 deg.C to obtain culture medium;
s2, inoculation and culture
Inoculating the Bacillus pasteurii seed liquid into the culture medium according to the inoculation amount of 0.5-2.0%, and culturing at constant temperature of 30 ℃ and the stirring speed of 180rpm for 3-7 days; introducing compressed air for aerobic culture, wherein the aeration ratio is 1:0.5(V/V.min) at the early stage of culture, when the relative dissolved oxygen is less than 50%, the aeration ratio is increased to more than 1:1(V/V.min), and continuously feeding a glucose solution with the mass concentration of 25% as a supplementing material; adjusting the flow rate of the compressed air to keep more than 30% of the relative dissolved oxygen, stopping feeding when the relative dissolved oxygen continuously rises, feeding ammonia water in the whole culture process to keep the pH value constant at 8.0, and obtaining a pasteurella culture solution after the culture is finished; diluting the Paenibacillus pasteurianus culture solution to OD600 of 0.6-1.2 for later use;
s3 mineralization enhancement
Soaking the concrete regenerated particles in the pasteuria bacillus culture solution diluted in the step S2 for 1-3h, and then carrying out mineralization treatment, wherein in the mineralization process, the pasteuria bacillus culture solution is circulated through a feed liquid pump, and urea and calcium nitrate are added simultaneously, so that a mineralized solution containing 0.6-0.9mol/L of urea and calcium nitrate is obtained, and the temperature of the mineralized solution is 25-30 ℃;
s4, drying
And (5) drying the mineralization solution obtained in the step (S3) to obtain the regenerated mineralization particles with the water content of 8-9%.
2. A method of reinforcing recycled concrete according to claim 1, wherein: in step S2, the glucose solution is fed with a supplement in an amount of 50-100ml/2.5L per hour, and the supplement is fed for 6-10 h.
3. A method of reinforcing recycled concrete according to claim 1, wherein: in step S1, the amount of the medium charged is 50%.
4. A method of reinforcing recycled concrete according to claim 1, wherein: in the mineralization process, after the concrete regenerated particles in the first mineralization pool are soaked and mineralized for 2 hours, the mineralization solution is emptied into the other mineralization pool to be soaked for 2 hours, and the cycle is performed for 12-20 times in sequence.
5. A method of reinforcing recycled concrete according to claim 1, wherein: in step S1, the fermentation tank includes a tank body (1), a magnetic stirring device (3), a sterilizing lamp, a driving mechanism and a tank door arranged on one side of the tank body (1), a steam pipe (5) and an exhaust valve (6) are arranged on the tank body (1), the steam pipe (5) is inserted into the tank body (1) from top to bottom, an air outlet hole (2) is formed in the circumferential surface of the steam pipe (5), and the magnetic stirring device (3) is installed at the bottom of the tank body (1);
the sterilizing lamp comprises a first sterilizing lamp (71) and a second sterilizing lamp (72), the first sterilizing lamp (71) and the second sterilizing lamp (72) are respectively positioned between the steam pipe (5) and the side wall of the tank body (1), and the driving mechanism is used for driving the first sterilizing lamp (71) and the second sterilizing lamp (72) to approach or separate from each other.
6. An automated high-density culture method of Paenibacillus pasteurii according to claim 5, wherein: the driving mechanism comprises a guide rod (81), a fixed plate (82), a moving table (83), a movable disc (84), a connecting rod (85) and an air cylinder (86);
the mobile station (83) comprises a first mobile station (831) and a second mobile station (832), the first sterilizing lamp (71) is installed on the first mobile station (831), the second sterilizing lamp (72) is installed on the second mobile station (832), the guide rod (81) is vertically arranged, two ends of the guide rod are respectively fixed on the side wall of the tank body (1), and the first mobile station (831) and the second mobile station (832) are respectively matched with the guide rod (81) in a sliding manner;
the fixing plate (82) is fixed in the middle of the guide rod (81), the first moving table (831) and the second moving table (832) are respectively positioned at two opposite sides of the fixing plate (82), a rotating shaft is arranged on one side of the fixing plate (82) close to the steam pipe (5), one end of the rotation is fixed on the fixed plate (82), the other end passes through the middle part of the movable disc (84), the link (85) includes a first link (851) and a second link (852), one end of the first connecting rod (851) is rotatably connected with the first mobile station (831), the other end is rotatably connected with the movable disk (84), one end of the second connecting rod (852) is rotatably connected with the second mobile station (832), and the other end is rotatably connected with the movable disk (84), the first connecting rod (851) and the second connecting rod (852) are respectively connected to two ends of the movable disc (84);
the air cylinder (86) is fixed to the top of the tank body (1), the air cylinder (86) comprises a piston rod, and the piston rod of the air cylinder (86) is fixed to the fixing plate (82).
7. An automated high-density culture method of Paenibacillus pasteurii according to claim 6, wherein: the first sterilizing lamp (71) and the second sterilizing lamp (72) are respectively provided with two sterilizing lamps, and the two sterilizing lamps (71) and the two sterilizing lamps (72) are respectively positioned at two opposite sides of the steam pipe (5).
8. An automated high-density culture method of Paenibacillus pasteurii according to claim 5, wherein: the air outlet holes (2) are arranged in a plurality, and the air outlet holes (2) are uniformly distributed on the surface of the steam pipe (5).
9. An automated high-density culture method of Paenibacillus pasteurii according to claim 5, wherein: the overlooking projection of the tank body (1) is circular, and the steam pipe (5) is vertically arranged and inserted into the bottom of the tank body (1).
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| WO2023099925A1 (en) * | 2021-12-05 | 2023-06-08 | Biozeroc Ltd | Methods of producing mineral based material |
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