CN113583838B - Rotary microorganism culture device - Google Patents
Rotary microorganism culture device Download PDFInfo
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- CN113583838B CN113583838B CN202110857022.XA CN202110857022A CN113583838B CN 113583838 B CN113583838 B CN 113583838B CN 202110857022 A CN202110857022 A CN 202110857022A CN 113583838 B CN113583838 B CN 113583838B
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- 244000005700 microbiome Species 0.000 title claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000003756 stirring Methods 0.000 claims abstract description 39
- 238000012544 monitoring process Methods 0.000 claims abstract description 30
- 235000015097 nutrients Nutrition 0.000 claims abstract description 28
- 238000005192 partition Methods 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 9
- 229910000838 Al alloy Inorganic materials 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 4
- 210000001503 joint Anatomy 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000003002 pH adjusting agent Substances 0.000 claims description 2
- 244000144985 peep Species 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims 2
- 239000010802 sludge Substances 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 8
- 238000005189 flocculation Methods 0.000 abstract description 6
- 230000016615 flocculation Effects 0.000 abstract description 6
- 239000002245 particle Substances 0.000 abstract description 6
- 238000007789 sealing Methods 0.000 abstract description 5
- 238000009434 installation Methods 0.000 abstract description 4
- 238000012423 maintenance Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 25
- 239000007788 liquid Substances 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 13
- 238000012258 culturing Methods 0.000 description 12
- 230000000593 degrading effect Effects 0.000 description 8
- 229910000831 Steel Inorganic materials 0.000 description 6
- 239000010959 steel Substances 0.000 description 6
- 229920000915 polyvinyl chloride Polymers 0.000 description 5
- 239000004800 polyvinyl chloride Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 4
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 4
- 238000005273 aeration Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 208000008918 voyeurism Diseases 0.000 description 3
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000004939 coking Methods 0.000 description 2
- 238000005056 compaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241001453382 Nitrosomonadales Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Abstract
The rotary microorganism culture device consists of a rotary drum, a stuffing box, a comprehensive pipe gallery, a pipe gallery supporting column, a rotary drum supporting wheel, a rotary drum driving wheel, a stirring column, an exhaust pipe, a water inlet and nutrient solution inlet pipe and a water quality monitoring signal cable; according to the rotary microorganism culture device, large-particle and compact anaerobic or aerobic granular sludge can be rapidly formed through rotation of the rotary drum, dead-angle-free stirring of the stirring column and vortex street flocculation of the stirring column, the sealing effect is good, a good anaerobic environment can be formed, no peculiar smell exists on site, the environment condition is good, the installation is simple, and the overhaul and the maintenance are easy.
Description
Technical Field
The invention relates to a microorganism culture technology, in particular to a rotary microorganism culture device.
Background
Conventional microorganism culture apparatuses are usually in the form of vessels, and are often used for research on adjustment of key parameters such as temperature, pH, dissolved oxygen, etc., and are configured to include an electric stirrer extending an impeller at the bottom end of a connecting rod into the bottom inside the vessel through the connecting rod. The electric impeller agitator usually has high rotation speed and high agitating force, so that the culture environment of the microorganism culture solution is destroyed. Relatively large microorganism culture devices employ shaker or tank structures. Some include a vertical drum with a stirrer, and stirring power density is uneven, and there is a stirring dead zone. The rotational speed of mixer is big, cuts mud floc, hurts microorganism, can not form granule mud.
Disclosure of Invention
Aiming at the problem of uneven stirring power density and dead stirring area, the invention adopts the rotary drum which can rotate horizontally, the microorganism culture liquid phase is always positioned at the lower half part of the rotary drum and is in good contact with the rotary drum, and the kinetic energy obtained by the culture liquid is uniform and no dead area exists.
Aiming at the problem of large cutting of sludge by the rotating speed of the traditional stirrer, the invention adopts the mode that a plurality of cylindrical stirring columns are arranged in the rotating drum and rotate in a ring shape under the rotation of the rotating drum, the rotating speed is lower than that of the traditional vertical stirrer, the sludge flocculation cannot be cut, and large-particle and compact granular sludge is easy to form.
The technical scheme of the invention is as follows:
the utility model provides a rotation type microorganism culture apparatus, its characterized in that, including the horizontal piping lane that sets up, the left end and the right-hand member of piping lane all are connected with the piping lane support column, be provided with between the left end and the right-hand member of piping lane around piping lane rotatory, and with the coaxial horizontal rotary drum of piping lane, be provided with the stirring post array on the inner wall of horizontal rotary drum, the left end and the right-hand member of horizontal rotary drum all are provided with annular protruding edge, and the protruding edge connection drive arrangement of one end annular, the protruding edge connection rotary drum supporting wheel of other end annular.
The utility model discloses a water quality monitoring signal cable, including utility tunnel, intake and nutrient solution pipe, water quality monitoring signal cable, the left port of intake passes the lateral wall of utility tunnel extremely the top of utility tunnel, the right port of intake stretches out outside the right port of utility tunnel, intake and nutrient solution pipe's left port passes the lateral wall of utility tunnel extremely outside the utility tunnel, intake and nutrient solution pipe's right port stretches out outside the right port of utility tunnel, the left end of intake and nutrient solution pipe passes the lateral wall of utility tunnel extremely the below of utility tunnel is connected with the monitoring instrument sensor, the right-hand member of water quality monitoring signal cable stretches out outside the right port of utility tunnel.
The left port of the exhaust pipe is connected to the port downward through an elbow.
And a pH regulator tube, a heater cable and a liquid level meter cable are integrated in the utility tunnel.
The annular protruding edge of horizontal rotary drum right-hand member is in rotatable coupling with the rotary drum right end board of fixing on the piping lane, the upper half of rotary drum right end board is provided with the peep hole, the lower half of rotary drum right end board is provided with blow-down pipe and supernatant fluid discharge tube from bottom to top.
And a stuffing box is arranged on the outer side of the joint of the right end plate of the rotary drum and the pipe gallery.
And a stuffing box is arranged outside the joint of the left end plate of the rotary drum and the pipe gallery.
The horizontal rotary drum comprises an upper half rotary drum and a lower half rotary drum, and the upper half rotary drum and the lower half rotary drum are in butt joint through a flange.
The stuffing in the stuffing box has a multi-layer compacted structure.
The utility tunnel is a PVC or aluminum alloy pipe with upper and lower partitions, an exhaust pipe is arranged in the upper partition space, a water inlet and nutrient solution inlet pipe and a water quality monitoring signal cable are arranged in the lower partition space.
The invention has the following technical effects: the invention relates to a rotary microorganism culture device which consists of a horizontal rotary drum, a Stuffing box (stuffer box), a comprehensive pipe gallery, a pipe gallery supporting column, a rotary drum supporting wheel, a rotary drum driving wheel, a stirring column, an exhaust pipe, a water inlet and nutrient solution inlet pipe and a water quality monitoring signal cable, wherein large-particle and compact anaerobic or aerobic granular sludge can be rapidly formed through rotation of the rotary drum, dead-angle-free stirring of the stirring column and vortex street flocculation of the stirring column, the sealing effect is good, a good anaerobic environment can be formed, no peculiar smell is generated on site, the environmental condition is good, the installation is simple, and the overhaul and the maintenance are easy.
Compared with the prior art, the invention has the following characteristics: 1) The stirring strength of the stirring column is adjusted by adjusting the rotating speed of the rotating drum, and the large-particle dense anaerobic or aerobic granular sludge can be rapidly formed by the vortex street flocculation effect of the stirring column. 2) The sealing effect is good, a good anaerobic environment or an aerobic environment can be formed, and strict anaerobic bacteria culture is easy. 3) When the device is used for culturing aerobic bacteria, the air is exhausted to the treatment device through the suction pipe, no peculiar smell exists on site, and the environmental condition is good. 4) The rotary drum is assembled through the upper half rotary drum and the lower half rotary drum, so that the rotary drum is simple to install and easy to overhaul and maintain.
Drawings
FIG. 1 is a schematic diagram of a rotary microorganism culture apparatus embodying the present invention.
The reference numerals are listed below: 1-a horizontal rotary drum; 2-upper half rotating cylinder; 3-a lower half drum; 4-Stuffing box (stuffer box); 5-Utility tunnel; 6-piping lane support columns; 7-a drum supporting wheel (supporting the right annular flange of the drum); 8-a driving device (driving the left annular convex edge of the rotary drum); 9-stirring the column; 10-an exhaust pipe; 11-water inlet and nutrient solution inlet pipe; 12-a water quality monitoring signal cable; 13-scope; 14-a supernatant discharge line; 15-blow-down tube (for collecting highly concentrated strain).
Detailed Description
The invention is described below with reference to the accompanying drawings (fig. 1) and examples.
FIG. 1 is a schematic diagram of a rotary microorganism culture apparatus embodying the present invention. Referring to fig. 1, a rotary microorganism culture apparatus, including the horizontal piping lane that sets up, the left end and the right-hand member of piping lane all are connected with piping lane support column 6, be provided with between the left end and the right-hand member of piping lane around piping lane rotatory, and with the coaxial horizontal rotary drum 1 of piping lane, be provided with stirring column array on the inner wall of horizontal rotary drum 1 in order to form no dead angle stirring and vortex street flocculation, the left end and the right-hand member of horizontal rotary drum 1 all are provided with annular protruding edge, and the protruding edge of one end is connected drive arrangement 8 (the driving motor of drive wheel is speed governing motor, variable frequency control), and the protruding edge of other end annular connects rotary drum supporting wheel 7. The utility tunnel is utility tunnel 5, integrated in the utility tunnel 5 has exhaust tube 10, intake and advance nutrient solution pipe 11, and water quality monitoring signal cable 12, the left port of exhaust tube 10 passes utility tunnel 5's lateral wall extremely the top of utility tunnel 5, the right port of exhaust tube 10 stretches out outside the right port of utility tunnel 5, intake and advance nutrient solution pipe 11's left port passes utility tunnel 5's lateral wall extremely outside utility tunnel 5, intake and advance nutrient solution pipe 11's right port stretches out outside utility tunnel 5's right port, water quality monitoring signal cable 12's left end passes utility tunnel 5's lateral wall extremely utility tunnel 5's below is connected with the monitoring instrument sensor, water quality monitoring signal cable 12's right-hand member stretches out outside utility tunnel 5's right port.
The left port of the exhaust pipe 10 has a structure that the port is downward connected to the elbow. And a pH regulator pipe, a heater cable and a liquid level meter cable are integrated in the utility tunnel 5. The annular protruding edge of horizontal rotary drum 1 right-hand member is fixed rotary drum right end board on utility tunnel 5 is rotatable to be connected, the upper half of rotary drum right end board is provided with scope 13, the lower half of rotary drum right end board is provided with blow-down pipe 15 and supernatant discharge tube 14 from bottom to top. And a stuffing box 4 is arranged outside the joint of the right end plate of the rotary drum and the comprehensive pipe rack 5. And a stuffing box is arranged on the outer side of the joint of the rotary drum left end plate and the comprehensive pipe rack 5. The horizontal rotary drum 1 comprises an upper half rotary drum 2 and a lower half rotary drum 3, and the upper half rotary drum 2 and the lower half rotary drum 3 are in butt joint through flanges. The packing in the packing box 4 has a multi-layer compacted structure. The utility tunnel 5 is a PVC or aluminum alloy pipe with upper and lower partitions, an exhaust pipe 10 is arranged in the upper partition space, a water inlet and nutrient solution inlet pipe 11 and a water quality monitoring signal cable 12 are arranged in the lower partition space.
The invention relates to a microorganism culture device, which belongs to the field of environmental protection and water treatment, and the main body of the microorganism culture device is rotatable and is mainly used for culturing microorganisms and has the main functions of: 1. culturing anaerobic microorganisms such as anaerobic granular anaerobic ammonia oxidizing bacteria; 2. culturing aerobic microorganisms such as phenol degrading bacteria, quinoline degrading bacteria, polycyclic Aromatic Hydrocarbons (PAHs) degrading bacteria, and hydrocarbon degrading bacteria; 3. screening specific microorganisms by domestication culture.
Aiming at the problem of uneven stirring power density and dead stirring area, the invention adopts the rotary drum which can rotate horizontally, the microorganism culture liquid phase is always positioned at the lower half part of the rotary drum and is in good contact with the rotary drum, and the kinetic energy obtained by the culture liquid is uniform and no dead area exists. Aiming at the problem of large cutting of sludge by the rotating speed of the traditional stirrer, the invention adopts the mode that a plurality of cylindrical stirring columns are arranged in the rotating drum and rotate in a ring shape under the rotation of the rotating drum, the rotating speed is lower than that of the traditional vertical stirrer, the sludge flocculation cannot be cut, and large-particle and compact granular sludge is easy to form.
The rotary microorganism culture device consists of a rotary drum, a stuffing box, a comprehensive pipe gallery, a pipe gallery supporting column, a rotary drum supporting wheel, a rotary drum driving wheel, a stirring column, an exhaust pipe, a water inlet and nutrient solution inlet pipe and a water quality monitoring signal cable; the rotary drum consists of an upper half rotary drum and a lower half rotary drum; the stirring column is welded inside the rotary drum, the rotary drum is supported on the rotary drum supporting wheel and the driving wheel, the utility tunnel passes through the rotary drum and is supported on the pipe tunnel supporting column, the exhaust pipe passes through the utility tunnel to reach the upper part inside the rotary drum, the water inlet and nutrient solution inlet pipe passes through the utility tunnel to reach the middle lower part inside the rotary drum, and the water quality monitoring signal cable passes through the utility tunnel to enter the rotary drum to be connected with the monitoring instrument sensor; and the stuffing box is fixed at the intersection of the two circular planes of the rotary drum and the comprehensive pipe rack.
For the installation of making things convenient for the device, also be convenient for the installation and the maintenance of the sensor of device internal monitoring instrument, the shell of upper half rotary drum and lower half rotary drum is welded outward has the flange board of pair, forms the rotary drum through the rubber seal who presss from both sides in the flange board and bolt fastening, for guaranteeing the steady operation of device, the shell of upper half rotary drum and lower half rotary drum is welded outward has the semi-annular steel sheet to form an holistic annular steel sheet, holistic annular steel sheet supports in the recess of rotary drum supporting wheel and drive wheel. In order to ensure that a good stirring effect is formed in the device, a plurality of cylindrical stirring columns are welded in the upper half rotary drum and the lower half rotary drum, are uniformly arranged along the radial interval of the upper half rotary drum and the lower half rotary drum, and are staggered with the exhaust pipe, the water inlet and nutrient solution inlet pipe and the water quality monitoring signal cable.
In order to ensure the sealing effect and save the arrangement space, the utility tunnel is a PVC or aluminum alloy pipe with axial partition, a cylindrical type is arranged in the upper partition space, and a water inlet and nutrient solution inlet pipe and a water quality monitoring signal cable are arranged in the lower partition space of the upper partition space. The rotation speed of the device is adjusted according to working conditions, and the driving motor of the driving wheel is a speed regulating motor and is controlled in a variable frequency mode. In order to ensure strict anaerobic environment required by anaerobic culture, the stuffing in the stuffing box adopts multistage stuffing and multistage compaction. In order to adjust the pH, temperature and monitor the liquid level of the culture solution in the device, the utility tunnel can be further provided with a pH regulator tube, a heater cable, a liquid level meter cable and the like. In order to facilitate the observation of the conditions in the device, the acquisition of the cultured microorganisms and the emptying of the culture solution in the rotary drum, the middle part of the upper rotary drum is provided with a peeping mirror, the radial direction of the lower rotary drum is provided with a plurality of supernatant discharge pipes with valves, and the bottom of the lower rotary drum is provided with a blow-down pipe.
As shown in FIG. 1, the rotary microorganism culture device consists of a horizontal rotary drum 1, a stuffing box 4, a comprehensive pipe gallery 5, a pipe gallery support column 6, a rotary drum support wheel 7, a rotary drum driving wheel 8, a stirring column 9, an exhaust pipe 10, a water inlet and nutrient solution inlet pipe 11 and a water quality monitoring signal cable 12; the rotary drum consists of an upper half rotary drum 2 and a lower half rotary drum 3; the stirring column 9 is welded inside the horizontal rotary drum 1, the horizontal rotary drum 1 is supported on the rotary drum supporting wheel 7 and the driving wheel 8, the utility tunnel 5 passes through the horizontal rotary drum 1 and is supported on the pipe tunnel supporting column, the exhaust pipe 10 passes through the utility tunnel 5 to the upper part inside the horizontal rotary drum 1, the water inlet and nutrient solution inlet pipe 11 passes through the utility tunnel 5 to the middle lower part inside the horizontal rotary drum 1, the water quality monitoring signal cable 12 passes through the utility tunnel 5 to enter the inside of the horizontal rotary drum 1 and is connected with the monitoring instrument sensor; the stuffing box 4 is fixed at the intersection of two circular planes of the horizontal rotary drum 1 and the comprehensive pipe gallery 5.
The outer parts of the shells of the upper half rotating cylinder 2 and the lower half rotating cylinder 3 are welded with a pair of flange plates, the horizontal rotating cylinder 1 is formed by fixing a rubber sealing gasket and bolts clamped in the flange plates,
semi-annular steel plates are welded outside the shells of the upper half rotating cylinder 2 and the lower half rotating cylinder 3 to form an integral annular steel plate, and the integral annular steel plate is supported in grooves of the rotating cylinder supporting wheel 7 and the driving wheel 8.
The inside welding of the upper half rotary cylinder 2 and the lower half rotary cylinder 3 has numerous cylindrical stirring columns, evenly arranges along the radial interval of the upper half rotary cylinder 2 and the lower half rotary cylinder 3 and staggers with the exhaust pipe 10, the water inlet and nutrient solution inlet pipe 11 and the water quality monitoring signal cable 12.
The utility tunnel 5 is a PVC (Polyvinyl chloride ) or aluminum alloy pipe with axial partition, a cylindrical type, and a water inlet and nutrient solution inlet pipe 11 and a water quality monitoring signal cable 12 are arranged in the upper partition space and the lower partition space of the exhaust pipe 10.
The driving motor of the driving wheel 8 is a speed regulating motor and is controlled in a variable frequency manner.
The stuffing in the stuffing box 4 adopts multistage stuffing and multistage compaction.
The utility tunnel 5 may also be provided with pH adjuster tubes, heater cables, level gauge cables, etc.
The middle part of the upper half rotary cylinder 2 is provided with a peeping mirror 13, the radial direction of the lower half rotary cylinder 3 is provided with a plurality of supernatant discharging pipes 14 with valves, and the bottom of the lower half rotary cylinder 3 is provided with a blow-down pipe.
The process flow of the invention is as follows: the horizontal rotary drum 1 is assembled by an upper rotary drum 2 and a lower rotary drum 3 and is supported on a rotary drum supporting wheel 7 and a rotary drum driving wheel 8; the utility tunnel 5 passes through the center of the horizontal rotary drum 1, and two ends of the utility tunnel are supported on the pipe tunnel support column 6 and pass through; the stuffing box 4 and the horizontal rotary drum 1 are sealed; the water inlet and nutrient solution inlet pipe 11 is positioned at the middle lower part of the horizontal rotary drum 1 through the utility tunnel 5, the exhaust pipe 10 is positioned at the upper part of the horizontal rotary drum 1 through the air inlet of the utility tunnel 5, and the water quality monitoring signal cable 12 is connected with various sensors positioned in the horizontal rotary drum 1 through the utility tunnel 5, such as a pH sensor, a DO sensor, a temperature sensor, a liquid level sensor, a sludge concentration sensor and the like;
culturing anaerobic bacteria: the horizontal drum 1 is rotated by the drum driving wheel 8 before mother liquor is inoculated so that the upper half drum 2 is positioned at the upper part of the lower half drum 3. After the horizontal rotary drum 1 is adjusted in place, valves on the supernatant liquid discharge pipe 14 and the blow-down pipe 15 are guaranteed to be in a closed state, and the valve of the exhaust pipe 10 is located in an open state, inoculating mother liquor and dilution water are pumped into the water inlet and nutrient solution inlet pipe 11 and then enter the horizontal rotary drum 1, the liquid level in the horizontal rotary drum 1 can be observed through the peeping mirror 13, and the liquid level is located at a position of 100-200 mm from the lower edge of the comprehensive pipe gallery 5. After the liquid level rises to the set liquid level, a valve on the water inlet and nutrient solution inlet pipe 11 is closed, and a driving motor of the drum driving wheel 8 is started, so that the horizontal drum 1 can be automatically rotated, and meanwhile, the liquid in the drum is uniformly stirred by the stirring column 9 in the horizontal drum 1.
The device can be further provided with a heating device, a heating rod is arranged at the lower end of the comprehensive pipe rack 5 in the horizontal rotary drum 1, the stirring column 9 is avoided, a 220V or 380V power supply of a fungus culturing room is connected with a cable connected with the heating rod through the comprehensive pipe rack 5, and the temperature of the liquid level in the drum can be monitored on an upper computer in real time in the processes of inoculating mother liquor and culturing, and if necessary, the temperature can be regulated and controlled.
Under the conditions of proper temperature, DO (dissolved oxygen), pH, nutrient supply and stirring intensity, the microorganisms in the inoculated mother liquor are propagated in a large quantity, the large-particle flocs and granular sludge are formed under the vortex street effect of the stirring column 9, and when the flocs and the granular sludge reach a certain concentration, the device can stop running and stand for a period of time to separate mud from water. The supernatant is discharged through the supernatant discharge pipe 14, and the highly concentrated strain is collected through the blow-down pipe 15, thereby completing the amplification culture of the strain.
The device can be further provided with a static pressure type liquid level meter, the static pressure type liquid level meter probe is hung at the lower end of the utility tunnel 5 in the horizontal rotary drum 1, the stirring column 9 is avoided, a cable connected with the static pressure type liquid level meter probe is connected with a PLC device (PLC, programmable Logic Controller, programmable logic controller) of the bacteria culturing room through the utility tunnel 5, and liquid level can be monitored on an upper computer in real time in the processes of inoculating mother liquid and culturing, and dilution water is supplemented if necessary.
Culturing aerobic bacteria: an air supply pipe is arranged in the horizontal rotary drum 1, an aeration head is hung and arranged at the lower end of the utility tunnel 5 in the horizontal rotary drum 1, a stirring column 9 is avoided, the aeration pipe connected with the aeration head is connected with a blower of a bacteria culturing room through the utility tunnel 5, DO (Dissolved Oxygen) can be monitored on an upper computer in real time, and the start of the blower is controlled. The exhaust gas is collected and discharged or treated through the exhaust pipe 10.
Example 1: the anaerobic granular anaerobic ammonia oxidation bacteria are cultivated and used for an anaerobic ammonia oxidation pond of a sewage station of a coking plant, and the formed granular sludge is compact and has the diameter of 0.25-7 mm.
Example 2: culturing phenol degrading bacteria, quinoline degrading bacteria, polycyclic Aromatic Hydrocarbon (PAHs) degrading bacteria and hydrocarbon degrading bacteria, and applying the bacteria and the bacteria to an aerobic tank of a coking plant to form compact granular sludge with the diameter of 0.25-7 mm.
What is not described in detail in the present specification belongs to the prior art known to those skilled in the art. It is noted that the above description is helpful for a person skilled in the art to understand the present invention, but does not limit the scope of the present invention. Any and all such equivalent substitutions, modifications and/or deletions as may be made without departing from the spirit and scope of the invention.
Claims (7)
1. The utility model provides a rotation type microorganism culture apparatus, its characterized in that includes the horizontal pipe rack that sets up, the left end and the right-hand member of pipe rack all are connected with the pipe rack support column, be provided with between the left end and the right-hand member of pipe rack around pipe rack rotatory and with the coaxial horizontal rotary drum of pipe rack, be provided with the stirring post array on the inner wall of horizontal rotary drum, the left end and the right-hand member of horizontal rotary drum all are provided with annular protruding edge, and the protruding edge connection drive arrangement of one end annular, the protruding edge connection rotary drum supporting wheel of the other end annular;
the horizontal rotary drum comprises an upper half rotary drum and a lower half rotary drum, and the upper half rotary drum and the lower half rotary drum are in butt joint through a flange;
a plurality of cylindrical stirring columns are welded inside the upper half rotary drum and the lower half rotary drum, and the cylindrical stirring columns are uniformly arranged along the radial interval of the upper half rotary drum and the lower half rotary drum;
the utility tunnel is characterized in that an exhaust pipe, a water inlet and nutrient solution inlet pipe and a water quality monitoring signal cable are integrated in the utility tunnel, the left port of the exhaust pipe penetrates through the side wall of the utility tunnel to the upper side of the utility tunnel, the right port of the exhaust pipe stretches out of the right port of the utility tunnel, the left port of the water inlet and nutrient solution inlet pipe penetrates through the side wall of the utility tunnel to the outside of the utility tunnel, the right port of the water inlet and nutrient solution inlet pipe stretches out of the right port of the utility tunnel, the left end of the water quality monitoring signal cable penetrates through the side wall of the utility tunnel to the lower side of the utility tunnel to be connected with a monitoring instrument sensor, and the right end of the water quality monitoring signal cable stretches out of the right port of the utility tunnel;
the annular protruding edge of horizontal rotary drum right-hand member is in rotatable coupling with the rotary drum right end board of fixing on the piping lane, the upper half of rotary drum right end board is provided with the peep hole, the lower half of rotary drum right end board is provided with blow-down pipe and supernatant fluid discharge tube from bottom to top.
2. The rotary microorganism culture apparatus according to claim 1, wherein the left port of the suction pipe has a structure connected to the port downward through a bend.
3. The rotating microbial cultivation apparatus according to claim 1, wherein the utility tunnel has integrated therein a pH adjuster tube, a heater cable and a level gauge cable.
4. The rotary microorganism culture apparatus according to claim 1, wherein a stuffing box is provided outside a junction of the right end plate of the drum and the pipe lane.
5. The rotary microorganism culture apparatus according to claim 4, wherein a stuffing box is provided outside a junction of the left end plate of the drum and the pipe lane.
6. The rotary microbial cultivation apparatus according to claim 5, wherein the packing in the packing box has a multi-layered compacted structure.
7. The rotary microorganism culture apparatus according to claim 1, wherein the utility tunnel is a PVC or aluminum alloy pipe with upper and lower partitions, an exhaust pipe is disposed in the upper partition space, and a water inlet and nutrient solution pipe and a water quality monitoring signal cable are disposed in the lower partition space.
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