CN113575503A - Candida albicans immunoreaction mouse molding device - Google Patents
Candida albicans immunoreaction mouse molding device Download PDFInfo
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- CN113575503A CN113575503A CN202110712874.XA CN202110712874A CN113575503A CN 113575503 A CN113575503 A CN 113575503A CN 202110712874 A CN202110712874 A CN 202110712874A CN 113575503 A CN113575503 A CN 113575503A
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- 238000000465 moulding Methods 0.000 title claims abstract description 87
- 241000222122 Candida albicans Species 0.000 title claims abstract description 44
- 229940095731 candida albicans Drugs 0.000 title claims abstract description 44
- 230000036046 immunoreaction Effects 0.000 title claims abstract description 36
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 54
- 230000007246 mechanism Effects 0.000 claims abstract description 42
- 238000005406 washing Methods 0.000 claims abstract description 29
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 43
- 239000001301 oxygen Substances 0.000 claims description 43
- 229910052760 oxygen Inorganic materials 0.000 claims description 43
- 239000007789 gas Substances 0.000 claims description 26
- 238000005485 electric heating Methods 0.000 claims description 13
- 241000883990 Flabellum Species 0.000 claims description 11
- 230000000903 blocking effect Effects 0.000 claims description 10
- 239000003814 drug Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
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- 208000033065 inborn errors of immunity Diseases 0.000 abstract 2
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- 238000010172 mouse model Methods 0.000 description 12
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- 238000000034 method Methods 0.000 description 7
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- 238000001514 detection method Methods 0.000 description 6
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
Abstract
The invention discloses a candida albicans immunoreaction mouse molding device, which comprises a molding incubator; through setting up modeling incubator and first drawer, during the mouse molding, place the inside at first drawer with the mouse, then insert first drawer in the inside of modeling incubator, make the modeling incubator provide normal living environment for the mouse of hypoimmunity, make the mouse survive in best growing environment, be favorable to candida albicans protein Enol, Bgl2, Pgk immunoreaction research's going on, through setting up modeling incubator and mechanism of admitting air, set up the top at the modeling incubator with the mechanism of admitting air, the gas washing jar and the disinfection mechanism in the mechanism of admitting air wash and disinfect the air of input respectively, and then reduced impurity and bacterium etc. in the air and got into the inside of modeling incubator, and then be favorable to the mouse of hypoimmunity to grow, be applicable to candida albicans protein Enol, Bgl2, Pgk immunoreaction mouse molding work.
Description
Technical Field
The invention belongs to the technical field of candida albicans, and particularly relates to a candida albicans immunoreaction mouse molding device.
Background
Candida albicans is a saprophytic bacterium, normally lives in skin, mucous membrane, digestive tract and other organs of a human body, when the resistance of the organism is reduced, Candida albicans can be propagated, and the human body can be attacked when the resistance reaches a certain amount, so the Candida albicans is a conditional pathogen. Candida albicans may also be transmitted through public baths, bathtubs, bath towels, swimwear, clothing, medical devices and dressings, and the like.
At present, a systemic candida infected mouse model is adopted to evaluate the immunoreactivity of candida albicans protein enolase 1(Eno1), beta-glucosidase 2(Bgl2) and phosphoglycerol kinase 1(Pgk 1); after the mice have low immunity due to the intraperitoneal injection of cyclophosphamide, the systemic candida infected mouse model is prepared by the intraperitoneal injection of candida albicans SC 5314; analyzing the immunoreactivity of the recombinant Eno1, Bgl2 and Pgk1 proteins and serum antibodies of the model mice by an ELISA method and a western blot method; the result models that the edge of the mesentery of the mouse has beige fine particles, candida growing like mycelium exists in the abdominal cavity, and the growth of the candida can be detected in the kidney; after the mice are infected with Candida albicans for 6-8 days, Ig G antibodies resisting Eno1, Bgl2 and Pgk1 appear, and the titer peak appears at 20-24 days after infection; anti Eno1 antibody titers were highest, followed by anti Pgk1, and lowest anti Bgl 2; eno1 has the strongest immunoreactivity with mouse serum, and Bgl2 has weaker immunoreactivity; therefore, candida can effectively stimulate mice to produce Ig G antibodies of Eno1, Bgl2 and Pgk 1; the recombinant Eno1 protein has the strongest immunoreactivity and can be used for subsequent research, thereby having important significance for the immune research of the candida albicans protein.
Therefore, in the molding process, the immunity of the mouse is in a low state, so that the requirement on the culture condition of the mouse is strict, the existing mouse molding device is simple in structure and generally depends on indoor air conditions for culture, and the mouse is not favorable for survival under the low immunity, so that the progress of mouse molding is influenced, the research on the immunoreaction of candida albicans protein Enol, Bgl2 and Pgk is not favorable, and the candida albicans immunoreaction mouse molding device is provided.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the existing defects and provide a candida albicans immunoreaction mouse model forming device, so as to solve the problems that the existing mouse model forming device proposed in the background technology is simple in structure, usually depends on indoor air conditions for culture, and is not beneficial to the survival of mice under low immunity, so that the mouse model forming is influenced, and the research on candida albicans protein Enol, Bgl2 and Pgk immunoreaction is not facilitated.
In order to achieve the purpose, the invention provides the following technical scheme: candida albicans immunoreaction mouse molding device, including the molding incubator, molding incubator fixed connection is on the top of base, the inside sliding connection of molding incubator has first drawer, the molding incubator outside is provided with observation glass, molding incubator top end fixedly connected with air inlet mechanism, sliding connection has the second drawer between molding incubator bottom and the base, air inlet mechanism includes gas washing jar and disinfection mechanism, the inside top fixedly connected with of molding incubator goes out the air cover, inside temperature and humidity sensor and the oxygen concentration sensor of being provided with of molding incubator.
Preferably, the mechanism of admitting air still includes the screen panel that admits air, fan, shower nozzle and connecting pipe, mechanism one side of admitting air is provided with the screen panel that admits air, the inside one side fixedly connected with fan that is located the screen panel that admits air of the mechanism of admitting air, the output and the gas washing tank fixed connection of fan, the inside top fixedly connected with shower of the mechanism of admitting air, the bottom of shower evenly is provided with the shower nozzle, the shower nozzle all is located the inside of gas washing tank, one side fixedly connected with connecting pipe of fan is kept away from to the gas washing tank.
Preferably, disinfection mechanism includes disinfection jar, ultraviolet disinfection board, driving motor, flabellum and outlet duct, the upper end and the connecting pipe fixed connection of disinfection jar, the inside ultraviolet disinfection board that evenly is provided with of disinfection jar, disinfection jar bottom fixedly connected with driving motor, driving motor's output fixedly connected with flabellum, the flabellum is located the inside of disinfection jar, just the flabellum is located the below of ultraviolet disinfection board, the below that the outside of disinfection jar lower extreme is located the flabellum is provided with the outlet duct, the outlet duct other end and air outlet cover fixed connection.
Preferably, an electric heating device is arranged on the outer side of the air outlet cover, and an electric heating wire is arranged inside the air outlet cover.
Preferably, the inside oxygen cylinder that is provided with of second drawer, the output of oxygen cylinder is provided with the solenoid valve, the output of solenoid valve is provided with the flow valve, the flow valve other end communicates with the inside of moulding incubator each other.
Preferably, an ultrasonic humidifying device is arranged inside the molding incubator.
Preferably, the inside ARM microprocessor, data acquisition module and the controller module of being provided with of base, the base outside is provided with display screen module and operating button module, data acquisition module, controller module, display screen module and operating button module all with ARM microprocessor electric connection, data acquisition module respectively with temperature and humidity sensor and oxygen concentration sensor electric connection, the controller module respectively with fan, driving motor, electric heater unit, solenoid valve and ultrasonic humidifying device electric connection.
Preferably, the even fixedly connected with division board in first drawer, the bleeder vent has all been seted up to the inside of first drawer and division board, it has a plurality of subspace to separate through the bleeder vent inside the first drawer, the first drawer inboard corresponds a plurality of subspaces and is provided with the silo, first drawer outside fixedly connected with feeding block, the feeding block top corresponds a plurality of dog-house has been seted up to the silo, the first drawer outside is provided with the antiskid circle.
Preferably, the lower extreme of gas washing jar is the arc form, gas washing jar top fixedly connected with back flow, back flow other end fixedly connected with storage box, the inboard upper end fixedly connected with medicine delivery pump of storage box, the output fixedly connected with conveyer pipe of medicine delivery pump, the conveyer pipe other end and the one end fixed connection of shower, medicine delivery pump and controller module electric connection.
Preferably, the inner side of the washing gas tank close to one end of the fan is fixedly connected with a blocking plate, the blocking plate is internally provided with a plurality of dispersing holes, and the fan is arranged in the dispersing holes.
Compared with the prior art, the invention provides the candida albicans immunoreaction mouse molding device, which has the following beneficial effects:
1. according to the invention, the molding incubator and the first drawer are arranged, when a mouse is molded, the mouse is placed in the first drawer, and then the first drawer is inserted into the molding incubator, so that the molding incubator provides a normal living environment for the mouse with low immunity, the mouse can survive in an optimal growing environment, and the research on the immunoreaction of candida albicans protein Enol, Bgl2 and Pgk is facilitated;
2. according to the invention, the molding incubator and the air inlet mechanism are arranged, the air inlet mechanism is arranged at the top end of the molding incubator, and the air washing tank and the disinfection mechanism in the air inlet mechanism respectively wash and disinfect the input air, so that impurities, bacteria and the like in the air are reduced to enter the molding incubator, and further, the growth of mice with low immunity is facilitated, and the method is suitable for the molding work of mice with candida albicans proteins Enol, Bgl2 and Pgk immunoreaction;
3. according to the invention, the electric heating device and the ultrasonic humidifying device are arranged, the electric heating device is used for heating the air input into the molding incubator to a normal temperature state suitable for the survival of the mouse, and the ultrasonic humidifying device is used for adjusting the humidity inside the molding incubator, so that the temperature and humidity inside the molding incubator are ensured to be stable, and a comfortable living environment is provided for the molding mouse with low immunity;
4. according to the invention, the second drawer is arranged, the oxygen bottle is arranged in the second drawer, the electromagnetic valve is arranged at the output end of the oxygen bottle, the oxygen concentration in the molding incubator is detected under the action of the oxygen concentration sensor, and if the oxygen concentration is lower than the set optimal concentration value, the controller module controls the electromagnetic valve to be opened, so that the oxygen in the oxygen bottle is input into the molding incubator, the oxygen concentration in the molding incubator is further maintained, a proper breathing environment is provided for the molding mouse with low immunity, and the molding of the mouse is facilitated.
The device has the advantages that the structure is scientific and reasonable, the use is safe and convenient, and great help is provided for people.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention without limiting the invention in which:
FIG. 1 is a schematic perspective view of a Candida albicans immunoreaction mouse model building device provided by the present invention;
FIG. 2 is a schematic side view of an air inlet mechanism in the candida albicans immunoreaction mouse model building device provided by the invention;
FIG. 3 is a schematic view of the internal structure of a gas washing tank in the candida albicans immunoreaction mouse model building device provided by the invention;
FIG. 4 is a schematic view of the internal structure of a sterilization tank in a Candida albicans immunoreaction mouse model building device provided by the present invention;
FIG. 5 is a schematic view of the internal structure of a modeling incubator in the Candida albicans immunoreaction mouse modeling device provided by the present invention;
FIG. 6 is a schematic view of the internal structure of the base of the Candida albicans immunoreaction mouse model building device according to the present invention;
FIG. 7 is a schematic perspective view of a first drawer in a Candida albicans immunoreaction mouse molding device provided by the present invention;
FIG. 8 is a schematic structural diagram of a blocking plate in a Candida albicans immunoreaction mouse model building device provided by the present invention;
in the figure: the device comprises a base 1, a molding incubator 2, a first drawer 3, observation glass 4, an air inlet mechanism 5, a second drawer 6, a gas washing tank 7, a disinfection mechanism 8, an air outlet cover 9, an air inlet mesh enclosure 10, a fan 11, a spray pipe 12, a spray head 13, a connecting pipe 14, a disinfection tank 15, an ultraviolet disinfection plate 16, a driving motor 17, fan blades 18, an air outlet pipe 19, an electric heating device 20, an oxygen bottle 21, an electromagnetic valve 22, a flow valve 23, an ultrasonic humidifying device 24, a temperature and humidity sensor 25, an oxygen concentration sensor 26, an ARM microprocessor 27, a data acquisition module 28, a controller module 29, a display screen module 30, an operation button module 31, a partition plate 32, an air vent 33, a trough 34, a feeding table 35, an anti-skid ring 36, a backflow pipe 37, a storage tank 38, a conveying pipe 39, a baffle plate 40 and dispersion holes 41.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-8, the present invention provides a technical solution: the candida albicans immunoreaction mouse molding device comprises a molding incubator 2, the molding incubator 2 is fixedly connected to the top end of a base 1, a first drawer 3 is connected to the inside of the molding incubator 2 in a sliding mode, an observation glass 4 is arranged on the outer side of the molding incubator 2, the molding incubator 2 and the first drawer 3 are arranged, when a mouse is molded, the mouse is placed inside the first drawer 3, then the first drawer 3 is inserted into the molding incubator 2, the molding incubator 2 provides a normal living environment for the mouse with low immunity, the mouse can live in the best growing environment, the research on candida albicans protein Enol, Bgl2 and Pgk immunoreaction is facilitated, an air inlet mechanism 5 is fixedly connected to the top end of the molding incubator 2, a second drawer 6 is connected between the bottom end of the molding incubator 2 and the base 1 in a sliding mode, the air inlet mechanism 5 comprises an air washing tank 7 and a disinfection mechanism 8, make the inside top fixedly connected with venthole 9 of mould incubator 2, make the inside humiture sensor 25 and the oxygen concentration sensor 26 that is provided with of mould incubator 2, through setting up mould incubator 2 and air inlet mechanism 5, set up air inlet mechanism 5 on the top of mould incubator 2, wash gas pitcher 7 and disinfection mechanism 8 in the air inlet mechanism 5 wash the air of input and disinfect respectively, and then impurity and bacterium etc. in the air have been reduced and the inside of mould incubator 2 is made to the entering, and then the mouse that is favorable to immunity low grows, be applicable to candida albicans protein Enol, Bgl2, Pgk immunoreaction mouse mould work.
In the invention, preferably, the air inlet mechanism 5 further comprises an air inlet mesh enclosure 10, a fan 11, a spray pipe 12, a spray head 13 and a connecting pipe 14, the air inlet mesh enclosure 10 is arranged on one side of the air inlet mechanism 5, the fan 11 is fixedly connected on one side of the air inlet mesh enclosure 10 in the air inlet mechanism 5, the output end of the fan 11 is fixedly connected with the air washing tank 7, the spray pipe 12 is fixedly connected on the top end of the air inlet mechanism 5, the spray heads 13 are uniformly arranged at the bottom end of the spray pipe 12, the spray heads 13 are all positioned in the air washing tank 7, the connecting pipe 14 is fixedly connected on one side of the air washing tank 7 far away from the fan 11, the fan 11 sucks in outside air through the air inlet mesh enclosure 10, then the air is input into the air washer 7, the disinfectant is sprayed by the nozzle 13, so that the disinfectant sprays and disinfects the air passing through the air washer 7, meanwhile, dust in the air can be settled, and the sprayed air enters the disinfection mechanism 8 through the connecting pipe 14.
In the invention, preferably, the disinfection mechanism 8 comprises a disinfection tank 15, ultraviolet disinfection plates 16, a driving motor 17, fan blades 18 and an air outlet pipe 19, the upper end of the disinfection tank 15 is fixedly connected with a connecting pipe 14, the ultraviolet disinfection plates 16 are uniformly arranged in the disinfection tank 15, the bottom end of the disinfection tank 15 is fixedly connected with the driving motor 17, the output end of the driving motor 17 is fixedly connected with the fan blades 18, the fan blades 18 are positioned in the disinfection tank 15, the fan blades 18 are positioned below the ultraviolet disinfection plates 16, the outer side of the lower end of the disinfection tank 15 is positioned below the fan blades 18 and is provided with the air outlet pipe 19, the other end of the air outlet pipe 19 is fixedly connected with an air outlet cover 9, the connecting pipe 14 is positioned at the outer side of the upper end of the disinfection tank 15, the bottom end of the disinfection tank 15 is fixedly connected with the driving motor 17, the output end of the driving motor 17 is fixedly connected with the fan blades 18, the fan blades 18 are positioned in the disinfection tank 15, and a plurality of ultraviolet disinfection plates 16 are uniformly arranged above the fan blades 18 on the inner side of the disinfection tank 15, the air that gets into behind the disinfection jar 15 flows downwards under the traction of flabellum 18, and then disinfects the disinfection through a plurality of ultraviolet ray disinfection board 16 to the air, then gets into the inside of air outlet cover 9 through outlet duct 19 of disinfection jar 15 lower extreme to the inside of importing into moulding incubator 2 through the sterile air that disinfects uses.
In the present invention, preferably, an electric heating device 20 is disposed outside the air outlet cover 9, an electric heating wire is disposed inside the air outlet cover 9, air is heated by the heating wire inside the air outlet cover 9, so that the air fed into the molding incubator 2 is heated to a normal temperature suitable for the survival of the mouse, an oxygen cylinder 21 is disposed inside the second drawer 6, an electromagnetic valve 22 is disposed at an output end of the oxygen cylinder 21, an output end of the electromagnetic valve 22 is provided with a flow valve 23, the other end of the flow valve 23 is communicated with the inside of the molding incubator 2, an electromagnetic valve 22 is disposed at an output end of the oxygen cylinder 21, the oxygen concentration inside the molding incubator 2 is detected under the action of the oxygen concentration sensor 26, and if the oxygen concentration is lower than a set optimum concentration value, the controller module 29 controls the electromagnetic valve 22 to open, so as to feed the oxygen inside the oxygen cylinder 21 into the molding incubator 2, thereby maintaining the oxygen concentration inside the molding incubator 2, provides a proper breathing environment for the molding mouse with low immunity.
In the invention, preferably, the ultrasonic humidifying device 24 is arranged in the molding incubator 2, and the ultrasonic humidifying device 24 is used for adjusting the humidity in the molding incubator 2, so that the temperature and humidity in the molding incubator 2 are stable, and a comfortable living environment is provided for the molding mice with low immunity.
In the invention, preferably, an ARM microprocessor 27, a data acquisition module 28 and a controller module 29 are arranged inside a base 1, a display screen module 30 and an operation button module 31 are arranged outside the base 1, the data acquisition module 28, the controller module 29, the display screen module 30 and the operation button module 31 are all electrically connected with the ARM microprocessor 27, the data acquisition module 28 is respectively electrically connected with a temperature and humidity sensor 25 and an oxygen concentration sensor 26, the controller module 29 is respectively electrically connected with a fan 11, a driving motor 17, an electric heating device 20, an electromagnetic valve 22 and an ultrasonic humidifying device 24, the temperature and humidity and the oxygen concentration inside a molding incubator 2 are set through the operation button module 31 outside the base 1, the data acquisition module 28 acquires the detection data of the temperature and humidity sensor 25 and the oxygen concentration sensor 26 and is processed by the ARM microprocessor 27, the controller module 29 is used for controlling the fan 11, the driving motor 17, the electric heating device 20, the electromagnetic valve 22 and the ultrasonic humidifying device 24 to respond, the temperature and humidity sensor 25 is used for detecting the temperature and humidity of the input air, if the humidity is lower than a preset value, the ultrasonic humidifying device 24 is used for working, if the temperature is lower than the preset value, the controller module 29 is used for controlling the electric heating device 20 on one side of the air outlet cover 9 to work, a heating wire in the air outlet cover 9 is used for heating the air to the normal temperature, according to the detection result of the oxygen concentration sensor 26 on the oxygen concentration, if the concentration is lower than the preset value, the controller module 29 is used for controlling the electromagnetic valve 22 at the output end of the oxygen bottle 21 to be opened, the output end of the electromagnetic valve 22 is provided with a flow valve 23 which is adjusted in advance, so that the oxygen concentration in the molding incubator 2 is increased uniformly, in the process, the detection results of the temperature and humidity sensor 25 and the oxygen concentration sensor 26 are displayed through the display screen module 30 after being processed by the ARM microprocessor 27, the growth environment of the mouse during molding is convenient to monitor.
In the invention, preferably, the partition plates 32 are uniformly and fixedly connected inside the first drawer 3, the air holes 33 are respectively formed inside the first drawer 3 and the partition plates 32, a plurality of subspaces are partitioned inside the first drawer 3 through the air holes 33, the material troughs 34 are arranged inside the first drawer 3 and correspond to the plurality of subspaces, the feeding table 35 is fixedly connected outside the first drawer 3, a plurality of feeding ports are formed in the top end of the feeding table 35 and correspond to the material troughs 34, the anti-slip rings 36 are arranged outside the first drawer 3, the plurality of subspaces are partitioned inside the first drawer 3 through the partition plates 32 and are convenient to group, then the first drawer 3 is inserted into the molding incubator 2, and the anti-slip rings 36 arranged outside the first drawer 3 ensure that the sealing performance and the stability between the first drawer and the molding incubator 2 are good.
In the invention, preferably, the lower end of the scrubber tank 7 is arc-shaped, the top end of the scrubber tank 7 is fixedly connected with a return pipe 37, the other end of the return pipe 37 is fixedly connected with a storage tank 38, the upper end of the inner side of the storage tank 38 is fixedly connected with a drug delivery pump, the output end of the drug delivery pump is fixedly connected with a delivery pipe 39, the other end of the delivery pipe 39 is fixedly connected with one end of the spray pipe 12, the drug delivery pump is electrically connected with the controller module 29, the drug delivery pump in the storage tank 38 inputs the disinfectant into the spray pipe 12 through the delivery pipe 39, and then the disinfectant is sprayed through the spray head 13 at the bottom end of the spray pipe 12.
In the invention, preferably, the inner side of the end of the washing gas tank 7 close to the fan 11 is fixedly connected with a blocking plate 40, the blocking plate 40 is internally provided with a plurality of dispersion holes 41, the blocking plate 40 is arranged between the inner side of the washing gas tank 7 and the fan 11, and the blocking plate 40 is internally provided with a plurality of dispersion holes 41, so that air entering the washing gas tank 7 is dispersed under the action of the dispersion holes 41, and the speed of the air passing through the washing gas tank 7 is reduced.
The working principle and the using process of the invention are as follows: when the device is used, mice with low immunity after treatment are placed in the first drawer 3 according to groups, the interior of the first drawer 3 is divided into a plurality of subspaces through the partition plates 32 so as to be convenient for grouping, then the first drawer 3 is inserted into the molding incubator 2, the anti-slip ring 36 arranged on the outer side of the first drawer 3 ensures good tightness and stability between the anti-slip ring and the molding incubator 2, then the temperature, humidity and oxygen concentration in the molding incubator 2 are set through the operation button module 31 on the outer side of the base 1, the ARM microprocessor 27 starts the fan 11 in the air inlet mechanism 5 and the ultrasonic humidifying device 24 in the molding incubator 2 through the controller module 29 according to the set values, the fan 11 sucks in outside air through the air inlet mesh enclosure 10 and then inputs the air into the air washing tank 7, the blocking plate 40 is arranged between the inner side of the air washing tank 7 and the fan 11, and the baffle 40 is internally provided with a plurality of dispersion holes 41, so that the air entering the gas washing tank 7 is dispersed under the action of the dispersion holes 41, so as to reduce the speed of the air passing through the gas washing tank 7, meanwhile, a drug delivery pump in the storage tank 38 inputs disinfectant into the spray pipe 12 through a delivery pipe 39, the bottom end of the spray pipe 12 is fixedly connected with a spray head 13, the spray head 13 is positioned in the gas washing tank 7, and then the disinfectant is sprayed through the spray head 13, so that the disinfectant sprays and disinfects the air passing through the gas washing tank 7, meanwhile, dust in the air can be settled, the bottom of the gas washing tank 7 is arc-shaped, the disinfectant convenient to spray enters the storage tank 38 through a return pipe 37, so as to achieve the effect of recycling, the sprayed air enters the inside of a disinfection tank 15 in the disinfection mechanism 8 through a connecting pipe 14, the connecting pipe 14 is located outside the upper end of the sterilizing tank 15, the bottom end of the sterilizing tank 15 is fixedly connected with a driving motor 17, the output end of the driving motor 17 is fixedly connected with a fan blade 18, the fan blade 18 is located inside the sterilizing tank 15, and a plurality of ultraviolet disinfection plates 16 are uniformly arranged above the fan blade 18 inside the sterilizing tank 15, the air entering the sterilizing tank 15 flows downwards under the traction of the fan blade 18, and then is sterilized and disinfected by the ultraviolet disinfection plates 16, and then enters the inside of the air outlet cover 9 through an air outlet pipe 19 at the lower end of the sterilizing tank 15, the air outlet cover 9 is located at the top end inside the molding incubator 2, so that the sterilized and disinfected air is input into the inside of the molding incubator 2 for use, meanwhile, the data acquisition module 28 acquires the detection data of the temperature and humidity sensor 25 and the oxygen concentration sensor 26 and is processed by the ARM microprocessor 27, the controller responds through the controller module 29, the temperature and humidity sensor 25 detects the temperature and humidity of the input air, if the humidity is lower than the preset value, the ultrasonic humidifying device 24 works, if the temperature is lower than the preset value, the controller module 29 controls the electric heating device 20 on one side of the air outlet cover 9 to work at the moment, so that the heating wire inside the air outlet cover 9 heats the air to the normal temperature, according to the detection result of the oxygen concentration sensor 26 on the oxygen concentration, if the concentration is lower than the preset value, the controller module 29 controls the electromagnetic valve 22 at the output end of the oxygen bottle 21 to be opened, the output end of the electromagnetic valve 22 is provided with the flow valve 23 which is adjusted in advance, so that the oxygen concentration inside the molding incubator 2 is uniformly increased, in the process, the detection results of the temperature and humidity sensor 25 and the oxygen concentration sensor 26 are displayed through the ARM display screen module 30 after being processed by the microprocessor 27, growth environment when being convenient for model building to the mouse monitors, when feeding, throw in food through the feeder table 35 in the first drawer 3 outside, the feeder table 35 top corresponds silo 34 and has seted up the dog-house, the dog-house communicates with each other with silo 34, and then be convenient for feed in groups, make the mould incubator 2 communicate with each other through the dog-house with the external world simultaneously, in order to maintain the inside and outside pressure balance of mould incubator 2, structural design is reasonable, and can provide normal living environment when making the mould for the mouse, make the mouse survive in the best growth environment, be favorable to candida albicans protein Enol, Bgl2, Pgk immunoreaction research go on.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. Candida albicans immunoreaction mouse molding device, including molding incubator (2), its characterized in that: make mould incubator (2) fixed connection in the top of base (1), the inside sliding connection of making mould incubator (2) has first drawer (3), it is provided with observation glass (4) to make mould incubator (2) outside, make mould incubator (2) top fixedly connected with mechanism (5) that admits air, sliding connection has second drawer (6) between making mould incubator (2) bottom and base (1), it includes gas washer (7) and disinfection mechanism (8) to admit air mechanism (5), make the inside top fixedly connected with venture cover (9) of mould incubator (2), it is provided with temperature and humidity sensor (25) and oxygen concentration sensor (26) to make mould incubator (2) inside.
2. The candida albicans immunoreaction mouse molding device of claim 1, wherein: mechanism (5) admits air still includes air inlet screen panel (10), fan (11), shower (12), shower nozzle (13) and connecting pipe (14), mechanism (5) one side of admitting air is provided with air inlet screen panel (10), mechanism (5) inside one side fixedly connected with fan (11) that are located air inlet screen panel (10) of admitting air, the output and the gas washer jar (7) fixed connection of fan (11), the inside top fixedly connected with shower (12) of mechanism (5) of admitting air, the bottom of shower (12) evenly is provided with shower nozzle (13), shower nozzle (13) all are located the inside of gas washer jar (7), one side fixedly connected with connecting pipe (14) of fan (11) are kept away from in gas washer jar (7).
3. The candida albicans immunoreaction mouse molding device of claim 2, wherein: disinfection mechanism (8) are including disinfection jar (15), ultraviolet ray disinfection board (16), driving motor (17), flabellum (18) and outlet duct (19), the upper end and connecting pipe (14) fixed connection of disinfection jar (15), disinfection jar (15) inside evenly is provided with ultraviolet ray disinfection board (16), disinfection jar (15) bottom fixedly connected with driving motor (17), the output fixedly connected with flabellum (18) of driving motor (17), flabellum (18) are located the inside of disinfection jar (15), just flabellum (18) are located the below of ultraviolet ray disinfection board (16), the below that the outside of disinfection jar (15) lower extreme is located flabellum (18) is provided with outlet duct (19), outlet duct (19) other end and venture cover (9) fixed connection.
4. The candida albicans immunoreaction mouse molding device of claim 3, wherein: the outer side of the air outlet cover (9) is provided with an electric heating device (20), and an electric heating wire is arranged inside the air outlet cover (9).
5. The candida albicans immunoreaction mouse molding device of claim 1, wherein: the inside oxygen cylinder (21) that is provided with of second drawer (6), the output of oxygen cylinder (21) is provided with solenoid valve (22), the output of solenoid valve (22) is provided with flow valve (23), the flow valve (23) other end communicates with the inside of moulding incubator (2) each other.
6. The candida albicans immunoreaction mouse molding device of claim 5, wherein: an ultrasonic humidifying device (24) is arranged in the molding incubator (2).
7. The candida albicans immunoreaction mouse molding device of claim 1, wherein: base (1) inside ARM microprocessor (27), data acquisition module (28) and controller module (29) of being provided with, base (1) outside is provided with display screen module (30) and operating button module (31), data acquisition module (28), controller module (29), display screen module (30) and operating button module (31) all with ARM microprocessor (27) electric connection, data acquisition module (28) respectively with temperature and humidity sensor (25) and oxygen concentration sensor (26) electric connection, controller module (29) respectively with fan (11), driving motor (17), electric heater unit (20), solenoid valve (22) and ultrasonic humidifying device (24) electric connection.
8. The candida albicans immunoreaction mouse molding device of claim 7, wherein: the utility model discloses a drawer structure, including first drawer (3), even fixedly connected with division board (32) in first drawer (3) inside, bleeder vent (33) have all been seted up to the inside of first drawer (3) and division board (32), a plurality of subspace has been separated through bleeder vent (33) in first drawer (3) inside, first drawer (3) inboard corresponds a plurality of subspace and is provided with silo (34), first drawer (3) outside fixedly connected with feeding table (35), feeding table (35) top corresponds a plurality of dog-house has been seted up in silo (34), first drawer (3) outside is provided with antiskid circle (36).
9. The candida albicans immunoreaction mouse molding device of claim 1, wherein: the lower extreme of washing gas jar (7) is the arc form, washing gas jar (7) top fixedly connected with back flow (37), back flow (37) other end fixedly connected with storage box (38), the defeated medicine pump of upper end fixedly connected with of storage box (38) inboard, the output end fixedly connected with conveyer pipe (39) of defeated medicine pump, the one end fixed connection of conveyer pipe (39) other end and shower (12), defeated medicine pump and controller module (29) electric connection.
10. The candida albicans immunoreaction mouse molding device of claim 9, wherein: the inner side of one end, close to the fan (11), of the washing gas tank (7) is fixedly connected with a blocking plate (40), dispersing holes (41) are formed in the blocking plate (40), and a plurality of dispersing holes (41) are formed in the dispersing holes.
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Cited By (1)
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| CN114276902A (en) * | 2021-12-29 | 2022-04-05 | 无锡苏南试验设备有限公司 | Mould test box |
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