CN113564035A - Microbial cell separation detection device - Google Patents

Microbial cell separation detection device Download PDF

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Publication number
CN113564035A
CN113564035A CN202110819758.8A CN202110819758A CN113564035A CN 113564035 A CN113564035 A CN 113564035A CN 202110819758 A CN202110819758 A CN 202110819758A CN 113564035 A CN113564035 A CN 113564035A
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block
box
shaft
processing
displacement
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陈丽华
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a microbial cell separation and detection device, which structurally comprises an operation box, a closing door, a support and a control panel, wherein the support is arranged on the operation box, the operation box is movably connected with the support, the control panel is arranged on the operation box, the operation box is movably clamped with the control panel, the closing door is arranged on the operation box, the closing door is in clearance fit with the operation box, the operation box is provided with a liquid pumping box, a separation box, a centrifugal shaft, a liquid pumping pipe, a perfusion box, a detection body and an installation box, the device can extend through a striking structure, strikes a shell to enable the shell to generate vibration, and shakes down cells attached to the shell through the vibration acting force of the shell, a part of the separation box of the device is contacted with the perfusion box and drives the shell to rotate through the rotating shaft, the liquid in the contact end of the shell and the perfusion tank can be stirred to promote the movement of cells in the liquid, so that the cells on the perfusion tank can be prevented from being attached to the shell.

Description

Microbial cell separation detection device
Technical Field
The invention relates to the technical field of biological detection, in particular to a microbial cell separation and detection device.
Background
The microbial cell separation is a process of propagating living cells into different types, namely a process of dividing cells into two cells, wherein the cells before division are called mother cells, new cells formed after division are called daughter cells, and a microbial cell separation detection device is adopted for separation detection of the microbial cells;
the microbial cell separation detection device detects cells in a perfusion sample culture box, the perfusion sample culture box needs to firstly realize the separation of the cells and clear liquid by the methods of centrifugation outside a cell tank and membrane column interception during detection, the cells with heavy mass are thrown out of a shaft body under the matching of a centrifugal shaft by utilizing the quality difference of the cells and the clear liquid, and the cells are easy to attach to the wall of a shell of a separation structure when being thrown out, so that the cells cannot be perfused for sample culture due to long-time attachment.
Disclosure of Invention
Aiming at the defects of the prior art, the invention is realized by the following technical scheme: a microbial cell separation detection device structurally comprises an operation box, a closing door, a bracket and a control panel, wherein the bracket is arranged on the operation box, the operation box is movably connected with the bracket, the control panel is arranged on the operation box, the operation box is movably clamped with the control panel, the operation box is provided with the closing door, and the closing door is in clearance fit with the operation box;
the operation box is provided with a liquid pumping body, a separation box, a centrifugal shaft, a liquid pumping pipe, a perfusion box, a detection body and an installation box, the liquid pumping body is installed on the separation box, the separation box is connected with the liquid pumping body, the centrifugal shaft is arranged in the separation box and is embedded with the separation box, the liquid pumping pipe is arranged on the separation box, the liquid pumping pipe is in clearance fit with the separation box, the detection body is arranged in the installation box and is connected with the installation box, the perfusion box is arranged on the installation box, and the perfusion box is movably connected with the installation box.
As the further optimization of this technical scheme, the separator box is equipped with installation pipe, regulator, casing, treater, axis of rotation, be equipped with the axis of rotation on the installation pipe, axis of rotation and installation pipe swing joint, the regulator is installed inside the casing, casing and regulator activity block, be equipped with the treater on the installation pipe, treater and installation pipe clearance fit.
As a further optimization of the technical scheme, the rotating shaft is an arc connecting block, a circular hollow is arranged at the center of the rotating shaft, a protruding block is arranged on the circular hollow of the rotating shaft, a circular rotating groove is arranged on the mounting pipe, the protruding block of the rotating shaft is embedded in the circular rotating groove of the mounting pipe, the other end of the rotating shaft is connected with the shell, a hollow is arranged on the shell, and the diameter of the hollow of the shell is identical to the diameter of the pipe body of the mounting pipe.
As a further optimization of the technical scheme, the regulator is provided with an operator, a box body, a pushing cavity, an assembling shaft, a turnover block, a pushing block and a striking block, the pushing cavity is arranged inside the box body and movably clamped with the box body, the turnover block is arranged on the pushing cavity and clamped with the pushing cavity, the pushing block is arranged inside the pushing cavity and movably connected with the pushing cavity, the operator is arranged on the pushing cavity, the pushing cavity is connected with the operator, the striking block is arranged on the assembling shaft and is in clearance fit with the assembling shaft, the assembling shaft is an arc-shaped shaft body, the assembling shaft is arranged at the top of the pushing cavity, the striking block is provided with a connecting cavity, the connecting cavity of the connecting cavity is provided with a connecting column, the connecting cavity of the striking block is in sliding connection with the connecting column, the surface of the striking block is provided with an arc-shaped convex block, the automatic feeding device is characterized in that a circular hollowed opening is formed in the pushing cavity, the overturning block is movably connected with the pushing cavity through a movable shaft, a semicircular protruding block is arranged on the overturning block, and the diameter of the semicircular protruding block of the overturning block is identical to that of the circular hollowed opening of the pushing cavity.
As a further optimization of the technical scheme, a connecting plate is arranged inside the pushing cavity, a hollow-out opening is formed in the connecting plate of the pushing cavity, sliding grooves are formed in two sides of the pushing cavity, a sliding block is arranged on the pushing block, the sliding block of the pushing block is embedded in the sliding groove of the pushing cavity, a connecting plate is arranged on the sliding block of the pushing block, the connecting plate of the pushing block is connected with the sliding groove of the pushing cavity in a sliding mode, and a colloid layer is arranged on the surface of the connecting plate of the pushing block.
As a further optimization of the technical scheme, the manipulator is provided with a device body, a rolling body, a moving plate, a rotating block, a moving channel, a traction body, an assembly pipe and an extension block, the moving channel is arranged in the device body and movably clamped with the device body, the moving plate is arranged on the moving channel and slidably connected with the moving channel, the assembly pipe is arranged in the device body and embedded with the device body, the traction body is arranged on the device body, the device body is in clearance fit with the traction body, the extension block is arranged in the device body and movably clamped with the device body, the rotating block is arranged on the moving plate, the moving plate is connected with the rotating block, the rolling body is arranged in the device body and embedded with the rolling body, the other end of the assembly pipe is connected with a connecting plate of a pushing cavity, and an arc groove is arranged in the rolling body, the ball body connecting device is characterized in that a ball body connecting block is embedded in the arc groove of the rolling body, the opening diameter of the arc groove of the rolling body is smaller than that of the ball body connecting block, an opening is formed in the device body, a connecting plate is arranged on the extending block, the diameter of the connecting plate of the extending block is identical to that of the opening of the device body, a hollow groove is formed in the extending block, a sliding block is arranged on the hollow groove of the extending block, a connecting rod is arranged on the device body, a sliding track is arranged on the connecting rod of the device body, and the extending block is embedded in the sliding track of the connecting rod through the sliding block.
As a further optimization of the technical scheme, the traction body is a circular ring connecting block, one end of the traction body is provided with a connecting shaft, two sides of the traction body are provided with sliding rails, the connecting shaft of the traction body is embedded on the sliding rails, the circular ring connecting block of the traction body penetrates through a pipe body of the assembling pipe, the assembling pipe is a rubber pipe body, the moving plate is provided with a notch, the rotating block is embedded at the notch of the moving plate through a moving shaft, the moving shaft of the rotating block is provided with a hollowed-out opening, the pipe body of the assembling pipe is provided with a through hole, and the through hole of the assembling pipe is communicated with the hollowed-out opening on the moving shaft of the rotating block.
As a further optimization of the technical scheme, the processor is provided with a displacement plate, a displacement block, a displacement body, a processing block, a processing shaft and a processing body, wherein the displacement plate is provided with the displacement block, the displacement block is connected with the displacement plate in a sliding manner, the processing block is arranged in the displacement block and is embedded with the displacement block, the processing shaft is arranged on two sides of the processing block, the processing shaft is in clearance fit with the processing block, the displacement body is arranged on two sides of the processing block and is embedded with the processing block, the processing body is arranged on the displacement block, the displacement block is connected with the processing body, the connecting blocks are arranged on two sides of a connecting shaft of the processing block and are connected with the processing shaft in a sliding manner, the other end of the connecting shaft of the processing shaft is provided with an arc shaft body, the arc of the processing shaft is movably connected with the connecting shaft through the movable shaft, and an arc protruding block is arranged on the arc shaft body of the processing shaft, a movable cavity is arranged on the deflection body, a slideway is arranged in the movable cavity of the deflection body, a connecting plate is embedded in the movable cavity of the deflection body, one end of the deflection body connecting plate is provided with an arc-shaped groove, the radian of the arc-shaped groove of the deflection body is matched with the radian of the processing shaft arc block, the displacement block is provided with a slideway, the processing body is a long shaft connecting block and is embedded on the slideway of the displacement block through a pulley, the processing body is provided with a connecting cavity, the inside of the connecting cavity of the processing body is embedded with a square connecting block, a hollow-out opening is arranged at the center of the square connecting block of the processing body, a sliding rod is arranged on the connecting cavity of the processing body, the square connecting block of the processing body is connected with the sliding rod of the connecting cavity in a sliding mode, a protruding block is arranged at one end of the displacement block, and the protruding sliding block of the displacement block is embedded on the displacement plate.
Advantageous effects
Compared with the prior art, the microbial cell separation and detection device has the following advantages:
1. the invention can extend through the beating structure, beat the shell to make the shell vibrate, shake the cells attached to the shell through the vibration acting force of the shell, and the separation box of the device has a part which is contacted with the perfusion box, and the shell is driven to rotate through the rotating shaft, so that the liquid of the contact end of the shell and the perfusion box can be stirred, the movement of the cells in the liquid is promoted, and the cells on the perfusion box can be prevented from being attached to the shell.
2. According to the invention, the rotating block is pushed out of the device body through the moving structure, the rotating block rotates for a certain angle along the movable shaft connected with the moving block, the notch of the rotating block faces the wall body of the shell, then the gas in the cavity is pushed to the rotating block through the assembling pipe through the pushing structure of the pushing cavity, and the cells slide into the perfusion box through the blowing of the gas, so that the cells are prevented from being attached to the shell for a long time, and the perfusion culture sample can not be realized.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic view of the overall structure of a device for separating and detecting microbial cells according to the present invention.
Fig. 2 is a schematic structural diagram of the operation box of the invention.
Fig. 3 is a schematic structural view of the separation box of the present invention.
Fig. 4 is a schematic view of the regulator of the present invention.
Fig. 5 is a schematic structural diagram of the manipulator of the present invention.
FIG. 6 is a diagram of a processor according to the present invention.
In the figure: the device comprises an operation box 1, a closing door 2, a bracket 3, a control panel 4, a liquid pumping 1a, a separation box 1b, a centrifugal shaft 1c, a liquid pumping pipe 1d, a perfusion box 1e, a detection body 1f, a mounting box 1g, a mounting pipe b1, a regulator b2, a shell b3, a processor b4, a rotating shaft b5, an operator b21, a box body b22, a pushing cavity b23, an assembly shaft b24, an overturning block b25, a pushing block b26, a beating block b27, a device body 211, a rolling body 212, a moving plate 213, a rotating block 214, a moving channel 215, a pulling body 216, an assembly pipe 217, an extension block 218, a displacement plate b41, a displacement block b42, a displacement body b43, a processing block b44, a processing shaft b45 and a processing body b 46.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purposes and the effects of the invention easy to understand, the following description and the accompanying drawings further illustrate the preferred embodiments of the invention.
Example one
Referring to fig. 1-5, a microbial cell separation detection device structurally comprises an operation box 1, a closing door 2, a bracket 3 and a control panel 4, wherein the bracket 3 is mounted on the operation box 1, the operation box 1 is movably connected with the bracket 3, the control panel 4 is arranged on the operation box 1, the operation box 1 is movably clamped with the control panel 4, the operation box 1 is provided with the closing door 2, and the closing door 2 is in clearance fit with the operation box 1;
the operation box 1 is equipped with the liquid 1a of taking out, separator box 1b, centrifugal shaft 1c, liquid suction pipe 1d, perfusion box 1e, detection body 1f, install box 1g, the liquid 1a of taking out is installed on separator box 1b, separator box 1b is connected with liquid suction pipe 1a, separator box 1b is inside to be equipped with centrifugal shaft 1c, centrifugal shaft 1c and separator box 1b gomphosis, be equipped with liquid suction pipe 1d on separator box 1b, liquid suction pipe 1d and separator box 1b clearance fit, install box 1g is inside to be equipped with detection body 1f, detection body 1f is connected with install box 1g, be equipped with perfusion box 1e on the install box 1g, perfusion box 1e and install box 1g swing joint realize little biological cell separation and detect.
Separation box 1b is equipped with installation pipe b1, regulator b2, casing b3, treater b4, axis of rotation b5, be equipped with axis of rotation b5 on installation pipe b1, axis of rotation b5 and installation pipe b1 swing joint, regulator b2 is installed inside casing b3, casing b3 and regulator b2 activity block, be equipped with treater b4 on installation pipe b1, treater b4 and installation pipe b1 clearance fit realize the separation of cell.
The rotating shaft b5 is an arc connecting block, a circular hollowed-out opening is formed in the center of the rotating shaft b5, a protruding block is arranged on the circular hollowed-out opening of the rotating shaft b5, a circular rotating groove is formed in the mounting pipe b1, the protruding block of the rotating shaft b5 is embedded in the circular rotating groove of the mounting pipe b1, the other end of the rotating shaft b5 is connected with the casing b3, the hollowed-out opening is formed in the casing b3, and the diameter of the hollowed-out opening of the casing b3 is matched with the diameter of the pipe body of the mounting pipe b1, so that the rotation of the assembly structure is achieved.
The regulator b2 is provided with an operator b2, a box b2, a pushing cavity b2, an assembling shaft b2, an overturning block b2, a pushing block b2 and a beating block b2, the pushing cavity b2 is arranged inside the box b2, the pushing cavity b2 is movably clamped with the box b2, the overturning block b2 is arranged on the pushing cavity b2, the overturning block b2 is clamped and connected with the pushing cavity b2, the pushing block b2 is arranged inside the pushing cavity b2, the pushing block b2 is movably connected with the pushing cavity b2, the operator b2 is arranged on the pushing cavity b2, the pushing cavity b2 is connected with the operator b2, the beating block b2 is arranged on the assembling shaft b2, the beating block b2 is in clearance fit with the assembling shaft b2, the assembling shaft b2 is an arc shaft body, the beating shaft b2 is arranged on the assembling shaft b2, a connecting column is arranged inside the beating block b2, and a connecting column of the assembling shaft b2 is connected with the beating block b2, and a connecting column is connected with the beating block b2, the surface of the striking block b27 is provided with an arc-shaped convex block, a circular hollowed-out opening is formed in the pushing cavity b23, the overturning block b25 is movably connected with the pushing cavity b23 through a movable shaft, a semicircular convex block is arranged on the overturning block b25, the diameter of the semicircular convex block of the overturning block b25 is identical to the diameter of the circular hollowed-out opening of the pushing cavity b23, and therefore dropping of attachments is achieved.
The inside connecting plate that is equipped with of propelling movement chamber b23, be equipped with the fretwork mouth on the connecting plate of propelling movement chamber b23, the both sides of propelling movement chamber b23 are equipped with sliding groove, be equipped with the slider on propelling movement piece b26, the slider gomphosis of propelling movement piece b26 is on the sliding groove of propelling movement chamber b23, be equipped with the connecting plate on the propelling movement piece b26 slider, the connecting plate of propelling movement piece b26 and the sliding groove sliding connection of propelling movement chamber b23, the connecting plate surface of propelling movement piece b26 is equipped with the colloid layer, and the gas of intracavity is released through the propelling movement structure.
The manipulator b21 is provided with a body 211, a rolling body 212, a moving plate 213, a rotating block 214, a moving channel 215, a pulling body 216, an assembly tube 217 and an extension block 218, the moving channel 215 is arranged in the body 211, the moving channel 215 is movably clamped with the body 211, the moving plate 213 is arranged on the moving channel 215, the moving plate 213 is connected with the moving channel 215 in a sliding way, the assembly tube 217 is arranged in the body 211, the assembly tube 217 is embedded with the body 211, the pulling body 216 is arranged on the body 211, the body 211 is in clearance fit with the pulling body 216, the extension block 218 is arranged in the body 211, the extension block 218 is movably clamped with the body 211, the rotating block 214 is arranged on the moving plate 213, the moving plate 213 is connected with the rotating block 214, the rolling body 212 is arranged in the body 211, the body 211 is embedded with the rolling body 212, the other end of the assembly tube 217 is connected with a connecting plate of a pushing cavity b23, the inside circular arc recess that is equipped with of rolling element 212, the gomphosis has the sphere connecting block on the circular arc recess of rolling element 212, the opening diameter of rolling element 212 circular arc recess is less than the opening diameter of sphere connecting block, be equipped with the opening on the ware body 211, be equipped with the connecting plate on the extension piece 218, the diameter of extension piece 218 connecting plate is identical with ware body 211 opening diameter, the inside of extension piece 218 is equipped with the fretwork groove, be equipped with the slider on the fretwork groove of extension piece 218, be equipped with the connecting rod on the ware body 211, be equipped with the slip track on the connecting rod of ware body 211, the adjustment of blowing angle is realized to extension piece 218 through the slider gomphosis on the slip track of connecting rod.
The pulling body 216 is a circular connecting block, a connecting shaft is arranged at one end of the pulling body 216, sliding rails are arranged on two sides of the pulling body 216, the connecting shaft of the pulling body 216 is embedded on the sliding rails, the circular connecting block of the pulling body 216 penetrates through a tube body of the assembling tube 217, the assembling tube 217 is a rubber tube body, a notch is formed in the moving plate 213, the rotating block 214 is embedded at the notch of the moving plate 213 through a moving shaft, a hollow-out hole is formed in the moving shaft of the rotating block 214, a through hole is formed in the tube body of the assembling tube 217, and the through hole of the assembling tube 217 is communicated with the hollow-out hole in the moving shaft of the rotating block 214, so that the pulling of the tube body is realized.
Starting a power supply, a part of a shell b3 of a separation box 1b is in contact with liquid of a perfusion box 1e during separation, cells on the perfusion box 1e are easy to attach to a shell b3, after the device separates clear liquid, the water level of the perfusion box 1e is lowered, the attached cells are not easy to fall off, therefore, before that, a rotating shaft b5 rotates along a rotating groove on a mounting tube b1 to drive the shell b3 to rotate integrally, the liquid stirred by a contact end of the shell b3 is prevented from attaching to the cells, during the rotation of the shell b3, a connecting plate of an extension block 218 slides along a connecting rod of a device body 211 to open an opening of the device body 211, 213 moves along a moving channel 215 to push a rotating block 214 out of the device moving plate 211, the rotating block 214 rotates along a moving angle connected with a moving shaft connected with the rotating block 213 to blow the opening towards the wall body of the shell b3, the assembly tube 217 is driven when the rotating block 214 moves, the body of the assembly tube 217 is in contact with the ball connecting block of the rolling body 212, the ball connecting block of the rolling body 212 rolls along the driving direction of the assembly tube 217, so that the abrasion of the assembly tube 217 is reduced, the slider of the pushing block b26 on the box b22 slides along the sliding groove of the pushing cavity b23 to push the connecting plate on the slider to slide, the connecting plate on the slider extrudes the gas in the pushing cavity b23, the gas flows to the rotating block 214 through the assembly tube 217, the attached cells are pushed by the blowing of the gas, the overturning block b25 rotates along the movable shaft connected with the pushing cavity b23 to open the cavity opening of the pushing cavity b23 for gas absorption, when the rotating block 214 is reset into the body 211, the pulling body 216 slides along the sliding tracks on two sides, the assembly tube 217 is reset to prevent the assembly tube 217 from being twisted and dragged together, the connecting column of the striking block b27 on the assembly shaft b24 extends along the connecting cavity in a sliding way, the casing b3 is continuously hit to generate vibration in the casing b3, so that the cells attached to the casing b3 are shaken off, the cells are shaken off by the vibration and blown off by gas, the attached cells do not need to be scraped off by a scraping structure, and the device does not puncture and damage the cells due to the scraping structure.
Example two
Referring to fig. 1-6, a microbial cell separation detection device structurally comprises an operation box 1, a closing door 2, a bracket 3 and a control panel 4, wherein the bracket 3 is mounted on the operation box 1, the operation box 1 is movably connected with the bracket 3, the control panel 4 is arranged on the operation box 1, the operation box 1 is movably clamped with the control panel 4, the operation box 1 is provided with the closing door 2, and the closing door 2 is in clearance fit with the operation box 1;
the operation box 1 is equipped with the liquid 1a of taking out, separator box 1b, centrifugal shaft 1c, liquid suction pipe 1d, perfusion box 1e, detection body 1f, install box 1g, the liquid 1a of taking out is installed on separator box 1b, separator box 1b is connected with liquid suction pipe 1a, separator box 1b is inside to be equipped with centrifugal shaft 1c, centrifugal shaft 1c and separator box 1b gomphosis, be equipped with liquid suction pipe 1d on separator box 1b, liquid suction pipe 1d and separator box 1b clearance fit, install box 1g is inside to be equipped with detection body 1f, detection body 1f is connected with install box 1g, be equipped with perfusion box 1e on the install box 1g, perfusion box 1e and install box 1g swing joint realize little biological cell separation and detect.
Separation box 1b is equipped with installation pipe b1, regulator b2, casing b3, treater b4, axis of rotation b5, be equipped with axis of rotation b5 on installation pipe b1, axis of rotation b5 and installation pipe b1 swing joint, regulator b2 is installed inside casing b3, casing b3 and regulator b2 activity block, be equipped with treater b4 on installation pipe b1, treater b4 and installation pipe b1 clearance fit realize the separation of cell.
The rotating shaft b5 is an arc connecting block, a circular hollowed-out opening is formed in the center of the rotating shaft b5, a protruding block is arranged on the circular hollowed-out opening of the rotating shaft b5, a circular rotating groove is formed in the mounting pipe b1, the protruding block of the rotating shaft b5 is embedded in the circular rotating groove of the mounting pipe b1, the other end of the rotating shaft b5 is connected with the casing b3, the hollowed-out opening is formed in the casing b3, and the diameter of the hollowed-out opening of the casing b3 is matched with the diameter of the pipe body of the mounting pipe b1, so that the rotation of the assembly structure is achieved.
The regulator b2 is provided with an operator b2, a box b2, a pushing cavity b2, an assembling shaft b2, an overturning block b2, a pushing block b2 and a beating block b2, the pushing cavity b2 is arranged inside the box b2, the pushing cavity b2 is movably clamped with the box b2, the overturning block b2 is arranged on the pushing cavity b2, the overturning block b2 is clamped and connected with the pushing cavity b2, the pushing block b2 is arranged inside the pushing cavity b2, the pushing block b2 is movably connected with the pushing cavity b2, the operator b2 is arranged on the pushing cavity b2, the pushing cavity b2 is connected with the operator b2, the beating block b2 is arranged on the assembling shaft b2, the beating block b2 is in clearance fit with the assembling shaft b2, the assembling shaft b2 is an arc shaft body, the beating shaft b2 is arranged on the assembling shaft b2, a connecting column is arranged inside the beating block b2, and a connecting column of the assembling shaft b2 is connected with the beating block b2, and a connecting column is connected with the beating block b2, the surface of the striking block b27 is provided with an arc-shaped convex block, a circular hollowed-out opening is formed in the pushing cavity b23, the overturning block b25 is movably connected with the pushing cavity b23 through a movable shaft, a semicircular convex block is arranged on the overturning block b25, the diameter of the semicircular convex block of the overturning block b25 is identical to the diameter of the circular hollowed-out opening of the pushing cavity b23, and therefore dropping of attachments is achieved.
The inside connecting plate that is equipped with of propelling movement chamber b23, be equipped with the fretwork mouth on the connecting plate of propelling movement chamber b23, the both sides of propelling movement chamber b23 are equipped with sliding groove, be equipped with the slider on propelling movement piece b26, the slider gomphosis of propelling movement piece b26 is on the sliding groove of propelling movement chamber b23, be equipped with the connecting plate on the propelling movement piece b26 slider, the connecting plate of propelling movement piece b26 and the sliding groove sliding connection of propelling movement chamber b23, the connecting plate surface of propelling movement piece b26 is equipped with the colloid layer, and the gas of intracavity is released through the propelling movement structure.
The manipulator b21 is provided with a body 211, a rolling body 212, a moving plate 213, a rotating block 214, a moving channel 215, a pulling body 216, an assembly tube 217 and an extension block 218, the moving channel 215 is arranged in the body 211, the moving channel 215 is movably clamped with the body 211, the moving plate 213 is arranged on the moving channel 215, the moving plate 213 is connected with the moving channel 215 in a sliding way, the assembly tube 217 is arranged in the body 211, the assembly tube 217 is embedded with the body 211, the pulling body 216 is arranged on the body 211, the body 211 is in clearance fit with the pulling body 216, the extension block 218 is arranged in the body 211, the extension block 218 is movably clamped with the body 211, the rotating block 214 is arranged on the moving plate 213, the moving plate 213 is connected with the rotating block 214, the rolling body 212 is arranged in the body 211, the body 211 is embedded with the rolling body 212, the other end of the assembly tube 217 is connected with a connecting plate of a pushing cavity b23, the inside circular arc recess that is equipped with of rolling element 212, the gomphosis has the sphere connecting block on the circular arc recess of rolling element 212, the opening diameter of rolling element 212 circular arc recess is less than the opening diameter of sphere connecting block, be equipped with the opening on the ware body 211, be equipped with the connecting plate on the extension piece 218, the diameter of extension piece 218 connecting plate is identical with ware body 211 opening diameter, the inside of extension piece 218 is equipped with the fretwork groove, be equipped with the slider on the fretwork groove of extension piece 218, be equipped with the connecting rod on the ware body 211, be equipped with the slip track on the connecting rod of ware body 211, the adjustment of blowing angle is realized to extension piece 218 through the slider gomphosis on the slip track of connecting rod.
The pulling body 216 is a circular connecting block, a connecting shaft is arranged at one end of the pulling body 216, sliding rails are arranged on two sides of the pulling body 216, the connecting shaft of the pulling body 216 is embedded on the sliding rails, the circular connecting block of the pulling body 216 penetrates through a tube body of the assembling tube 217, the assembling tube 217 is a rubber tube body, a notch is formed in the moving plate 213, the rotating block 214 is embedded at the notch of the moving plate 213 through a moving shaft, a hollow-out hole is formed in the moving shaft of the rotating block 214, a through hole is formed in the tube body of the assembling tube 217, and the through hole of the assembling tube 217 is communicated with the hollow-out hole in the moving shaft of the rotating block 214, so that the pulling of the tube body is realized.
The processor b4 is provided with a displacement plate b41, a displacement block b42, an offset body b43, a processing block b43, a processing shaft b43 and a processing body b43, the displacement plate b43 is provided with the displacement block b43, the displacement block b43 is slidably connected with the displacement plate b43, the processing block b43 is arranged inside the displacement block b43, the processing block b43 is embedded with the displacement block b43, the processing shaft b43 is arranged on two sides of the processing block b43, the processing shaft b43 is in clearance fit with the processing block b43, the offset body b43 is arranged on two sides of the processing block b43, the offset body b43 is embedded with the processing block b43, the processing body b43 is mounted on the displacement block b43, the displacement block b43 is connected with the processing body b43, connecting shafts are arranged on two sides of the processing block b43, connecting shafts are provided with connecting shafts, the connecting shafts of the processing block b43 are movably connected with arc shafts 43 through connecting shafts, and the processing shafts 43 are movably connected with the arc shafts 43, an arc convex block is arranged on an arc shaft body of the processing shaft b45, a movable cavity is arranged on the deviation body b43, a slide way is arranged inside the movable cavity of the deviation body b43, a connecting plate is embedded inside the movable cavity of the deviation body b43, an arc groove is arranged at one end of the connecting plate of the deviation body b43, the radian of the arc groove of the deviation body b43 is matched with the radian of the arc block of the processing shaft b45, a slide way is arranged on the displacement block b42, the processing body b46 is a long shaft connecting block, the processing body b46 is embedded on the slide way of the displacement block b42 through a pulley, a connecting cavity is arranged on the processing body b46, a square connecting block is embedded inside the connecting cavity of the processing body b46, a hollow hole is arranged at the center of the square connecting block of the processing body b46, a slide rod is arranged on the connecting cavity of the processing body b46, the square connecting block of the processing body b46 is in sliding connection with the slide rod of the connecting cavity, one end of the displacement block b42 is provided with a convex block, and a convex slide block of the displacement block b42 is embedded on the displacement plate b41 to dredge the assembled pipe orifice.
In the first embodiment, processor b4 is installed, when installation tube b1 absorbs liquid and separates, if installation tube b1 is blocked by collected cell tissue and the like, displacement block b42 slides along displacement plate b41, displacement plate b41 is pushed to the nozzle of installation tube b1, the connecting plate of displacement block b43 slides along the movable cavity, the opening of displacement plate b41 is opened, the connecting block of processing block b44 slides on processing shaft b45, processing block b44 is pushed out of displacement block b42, cell tissue at the blocking end is pushed through processing block b44, the blocked cell tissue is stirred, the collected cell tissue is squeezed out, so that installation tube b1 is dredged, the connecting cavity of processing block b46 slides on the sliding track of displacement block b42, the connecting cavity of processing block b46 is pushed out, the square connecting block of processing block b46 slides along the connecting cavity, further, installation tube b1 is hit by the vibration force 1 of installation tube b 53926, the cell tissue is shaken out of the blocking end so as to install the tube b1 for liquid suction, and the contact end of the processing block b44 is of a circular arc structure without a sharp edge so as not to easily puncture the cells.
While there have been shown and described what are at present considered the fundamental principles of the invention, the essential features and advantages thereof, it will be understood by those skilled in the art that the present invention is not limited by the embodiments described above, which are merely illustrative of the principles of the invention, but rather, is capable of numerous changes and modifications in various forms without departing from the spirit or essential characteristics thereof, and it is intended that the invention be limited not by the foregoing descriptions, but rather by the appended claims and their equivalents.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (8)

1. A microbial cell separation detection device, characterized in that: the structure of the device comprises an operation box (1), a closing door (2), a support (3) and a control panel (4), wherein the support (3) is installed on the operation box (1), the operation box (1) is movably clamped with the control panel (4), and the closing door (2) is arranged on the operation box (1);
the operation box (1) is equipped with and takes out liquid (1a), separator box (1b), centrifugal axis (1c), liquid suction pipe (1d), perfusion case (1e), detection body (1f), install box (1g), take out liquid (1a) and install on separator box (1b), centrifugal axis (1c) and separator box (1b) gomphosis, be equipped with liquid suction pipe (1d) on separator box (1b), detection body (1f) is connected with install box (1g), be equipped with perfusion case (1e) on install box (1 g).
2. The apparatus for separating and detecting microorganism cells according to claim 1, wherein: separator box (1b) is equipped with installation pipe (b1), regulator (b2), casing (b3), treater (b4), axis of rotation (b5), axis of rotation (b5) and installation pipe (b1) swing joint, inside casing (b3) was installed in regulator (b2), treater (b4) and installation pipe (b1) clearance fit.
3. The apparatus for separating and detecting microorganism cells according to claim 2, wherein: the rotating shaft (b5) is an arc connecting block, and a circular hollow is arranged at the center of the rotating shaft (b 5).
4. The apparatus for separating and detecting microorganism cells according to claim 2, wherein: regulator (b2) are equipped with operation ware (b21), box (b22), propelling movement chamber (b23), equipment axle (b24), upset piece (b25), propelling movement piece (b26), hit and beat piece (b27), box (b22) inside is equipped with propelling movement chamber (b23), upset piece (b25) is connected with propelling movement chamber (b23) block, propelling movement chamber (b23) internally mounted has propelling movement piece (b26), propelling movement chamber (b23) is connected with operation ware (b21), be equipped with on equipment axle (b24) and hit and beat piece (b 27).
5. The apparatus for separating and detecting microorganism cells according to claim 4, wherein: a connecting plate is arranged inside the pushing cavity (b23), and a hollow opening is formed in the connecting plate of the pushing cavity (b 23).
6. The apparatus for separating and detecting microorganism cells according to claim 4, wherein: the manipulator (b21) is provided with a manipulator body (211), a rolling body (212), a moving plate (213), a rotating block (214), a moving channel (215), a traction body (216), an assembly pipe (217) and an extension block (218), the moving channel (215) is arranged inside the manipulator body (211), the moving plate (213) is in sliding connection with the moving channel (215), the assembly pipe (217) is arranged inside the manipulator body (211), the manipulator body (211) is in clearance fit with the traction body (216), the extension block (218) is arranged inside the manipulator body (211), the moving plate (213) is connected with the rotating block (214), and the rolling body (212) is installed inside the manipulator body (211).
7. The apparatus for separating and detecting microorganism cells according to claim 6, wherein: the traction body (216) is a circular connecting block, and one end of the traction body (216) is provided with a connecting shaft.
8. The apparatus for separating and detecting microorganism cells according to claim 2, wherein: the processor (b4) is provided with a displacement plate (b41), a displacement block (b42), a displacement body (b43), a processing block (b44), a processing shaft (b45) and a processing body (b46), the displacement plate (b41) is provided with the displacement block (b42), the processing block (b44) is embedded with the displacement block (b42), the processing shaft (b45) is arranged on two sides of the processing block (b44), the displacement body (b43) is embedded with the processing block (b44), and the processing body (b46) is arranged on the displacement block (b 42).
CN202110819758.8A 2021-07-20 2021-07-20 Microbial cell separation detection device Withdrawn CN113564035A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110819758.8A CN113564035A (en) 2021-07-20 2021-07-20 Microbial cell separation detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110819758.8A CN113564035A (en) 2021-07-20 2021-07-20 Microbial cell separation detection device

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Publication Number Publication Date
CN113564035A true CN113564035A (en) 2021-10-29

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110819758.8A Withdrawn CN113564035A (en) 2021-07-20 2021-07-20 Microbial cell separation detection device

Country Status (1)

Country Link
CN (1) CN113564035A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115072896A (en) * 2022-05-30 2022-09-20 曹宗桥 Aquaculture tail water purification device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115072896A (en) * 2022-05-30 2022-09-20 曹宗桥 Aquaculture tail water purification device

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