CN113552251A - Method for detecting nucleotide in dietary supplement product based on nucleotide rapid nutrition supplement - Google Patents
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- 239000002773 nucleotide Substances 0.000 title claims abstract description 65
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 62
- 235000015872 dietary supplement Nutrition 0.000 title claims abstract description 27
- 239000000047 product Substances 0.000 title claims abstract description 22
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 14
- 230000035764 nutrition Effects 0.000 title claims abstract description 13
- 239000013589 supplement Substances 0.000 title claims abstract description 13
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 17
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical class O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 21
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 21
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 20
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical class N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 17
- 239000012086 standard solution Substances 0.000 claims description 9
- 238000010829 isocratic elution Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- TXOSAXQFTKBXLI-UHFFFAOYSA-N 3,7-dihydropurin-6-one;7h-purin-6-amine Chemical class NC1=NC=NC2=C1NC=N2.O=C1N=CNC2=C1NC=N2 TXOSAXQFTKBXLI-UHFFFAOYSA-N 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000010812 external standard method Methods 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 230000014759 maintenance of location Effects 0.000 description 10
- GRSZFWQUAKGDAV-UHFFFAOYSA-N Inosinic acid Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-UHFFFAOYSA-N 0.000 description 9
- 235000013902 inosinic acid Nutrition 0.000 description 9
- AUHDWARTFSKSAC-HEIFUQTGSA-N (2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-2-(6-oxo-1H-purin-9-yl)oxolane-2-carboxylic acid Chemical compound [C@]1([C@H](O)[C@H](O)[C@@H](CO)O1)(N1C=NC=2C(O)=NC=NC12)C(=O)O AUHDWARTFSKSAC-HEIFUQTGSA-N 0.000 description 8
- 229940028843 inosinic acid Drugs 0.000 description 8
- 239000004245 inosinic acid Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 4
- 229960000643 adenine Drugs 0.000 description 4
- 229940125904 compound 1 Drugs 0.000 description 4
- 229940104302 cytosine Drugs 0.000 description 4
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical class O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- 230000009469 supplementation Effects 0.000 description 4
- 229940035893 uracil Drugs 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical class O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a method for detecting nucleotide in a dietary supplement product based on nucleotide rapid nutrition supplement, which corrects a high performance liquid chromatography by adopting an external standard method, can directly analyze the dietary supplement product to be detected by using the high performance liquid chromatography, has no need of processing a sample, is simple to operate, has small sample dosage, high sensitivity and strong specificity, and can be used for the dietary supplement product based on nucleotide rapid nutrition supplement.
Description
Technical Field
The invention relates to the technical field of nutrient component detection, in particular to a method for detecting nucleotide in a dietary supplement product based on rapid nucleotide nutrition supplement.
Background
Aiming at the condition that the current population is susceptible to diseases, on the basis of ocean resources, an advanced biological extraction technology is adopted, the nutrient components and the taste of high-value food materials can be preserved after extraction, and a high-nutrient-value dietary supplement product which is rich in various nutrient substances, can promote the immunity of the organism and further improves the disease resistance is researched and developed. The nucleotide is a compound consisting of purine base or pyrimidine base, ribose or deoxyribose and phosphate, and comprises the following components: five nucleotides of CMP (cytosine nucleotide), UMP (uracil nucleotide), AMP (adenine nucleotide), GMP (guanine nucleotide) and IMP (inosinic acid). Nucleotide compounds have important biological functions, and they participate in almost all biochemical reactions in the body. Therefore, the detection of nucleotides in dietary supplements based on rapid nutritional supplementation of nucleotides is of particular importance. However, at present, there are few researches on the detection of five nucleotides in dietary supplements, so that it is necessary to develop a simple, efficient and reliable method for detecting nucleotides in dietary supplements based on rapid nutritional supplementation of nucleotides, so as to meet the production requirements.
Disclosure of Invention
The invention aims at the problems and provides a method for detecting nucleotide in a dietary supplement product based on nucleotide rapid nutrition supplement.
The technical scheme adopted by the invention for solving the problems is as follows: the method for detecting the nucleotides in the dietary supplement product based on the rapid nutrient supplement of the nucleotides comprises the following steps of detecting the nucleotides in the dietary supplement product based on the rapid nutrient supplement of the nucleotides, wherein the nucleotides are five nucleotides of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and inosine nucleotide; the detection method comprises the following steps:
the method comprises the following steps: respectively supplying standard solutions containing five nucleotides of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and hypoxanthine adenine nucleotide into the high performance liquid chromatography, and respectively establishing standard curves of different nucleotides by taking the concentration ratio of single nucleotide as an X axis and the peak area of the single nucleotide as a Y axis;
step two, taking a food supplement product sample frozen at minus 60 ℃ and based on nucleotide rapid nutrition supplement, unfreezing at normal temperature, taking a proper amount of sample in a 10mLEP tube, putting the tube into a high-speed centrifuge for 10min at 10000r/min, putting the tube into a 1.5mL automatic sample feeding bottle after passing through a 0.22um microporous filter membrane, putting the bottle into a 4 ℃ refrigerator for storage, and taking the bottle out before analysis;
and step three, supplying the sample to high performance liquid chromatography to obtain the concentration of the nucleotide.
Further, in the first step, the operating parameters of the high performance liquid chromatography are as follows: a chromatographic column: ZORBAXeclipsePlusC18(4.6 x 250mm 5-Micron); the detection wavelength is as follows: 254 nm; column temperature: 30 ℃; flow rate: 0.5 ml/min; sample introduction amount: 10 mu l of the mixture; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with the volume ratio of 95: 5; isocratic elution.
Further, in the second step, the working parameters of the high performance liquid chromatography are as follows: a chromatographic column: ZORBAXeclipsePlusC18(4.6 x 250mm 5-Micron); the detection wavelength is as follows: 254 nm; column temperature: 30 ℃; flow rate: 0.5 ml/min; sample introduction amount: 10 mu l of the mixture; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with the volume ratio of 95: 5; isocratic elution.
The invention has the advantages that:
(1) the method adopts an external standard method to correct the high performance liquid chromatography, can directly analyze the food supplement product to be detected by using the high performance liquid chromatography, has no need of processing a sample, is simple to operate, has small sample dosage, high sensitivity and strong specificity, and can be used for the food supplement product based on the rapid nucleotide nutrition supplement.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.
Drawings
FIG. 1 is a flow chart of nucleotide detection in dietary supplement products based on rapid nucleotide nutritional supplementation;
FIG. 2 is a chromatogram of a standard nucleotide solution 1;
FIG. 3 is a chromatogram of a standard nucleotide solution 2;
FIG. 4 is a chromatogram of a standard nucleotide solution 3;
FIG. 5 is a chromatogram of a standard nucleotide solution 4;
FIG. 6 is a chromatogram of a standard nucleotide solution 5;
FIG. 7 is a standard graph 1 of different nucleotides;
FIG. 8 is a standard graph 2 of different nucleotides;
FIG. 9 is a standard curve 3 for different nucleotides;
FIG. 10 shows the result 1 of detection of an unknown nucleotide concentration;
FIG. 11 shows the detection result 2 of an unknown nucleotide concentration.
Detailed Description
The following detailed description of embodiments of the invention, but the invention can be practiced in many different ways, as defined and covered by the claims.
Example 1
Method for detecting nucleotide in dietary supplement product based on nucleotide rapid nutrition supplement
1. Establishing a standard curve
The working parameters for controlling the high performance liquid chromatography are as follows: a chromatographic column: ZORBAXeclipsePlusC18(4.6 x 250mm 5-Micron); the detection wavelength is as follows: 254 nm; column temperature: 30 ℃; flow rate: 0.5 ml/min; sample introduction amount: 10 mu l of the mixture; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with the volume ratio of 95: 5; isocratic elution. And sequentially inputting standard solutions into the high performance liquid chromatography.
(1) Preparing standard solutions with the concentrations of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and inosinic acid being 10 mug/mL, inputting the standard solutions into a high performance liquid chromatography to obtain five retention times and corresponding peak areas, as shown in Table 1 and figure 2:
TABLE 1
| Peak number | Retention time | Area of | Height | Concentration of | Concentration unit | Compound (I)Name (name) | |
| 1 | 4.310 | 72221 | 7285 | 10.0000 | ug/ | Cytosine nucleotide | |
| 2 | 18.008 | 219505 | 6339 | 10.0000 | ug/ | Adenine nucleotide | |
| 3 | 19.194 | 104670 | 2924 | 10.0000 | ug/mL | |
|
| 4 | 23.110 | 154870 | 3557 | 10.0000 | ug/mL | Guanine |
|
| 5 | 27.379 | 168152 | 3331 | 10.0000 | ug/mL | Inosine A | |
| Total of | 719419 | 23436 |
(2) Inputting standard solutions with concentrations of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and inosinic acid of 20 μ g/mL, five retention times and corresponding peak areas were obtained, as shown in table 2 and fig. 3:
TABLE 2
| Peak number | Retention time | Area of | Height | Concentration of | Concentration unit | Name of |
|
| 1 | 4.313 | 137984 | 13807 | 20.0000 | ug/ | Cytosine nucleotide | |
| 2 | 18.026 | 420413 | 12072 | 20.0000 | ug/ | Adenine nucleotide | |
| 3 | 19.207 | 200903 | 5539 | 20.0000 | ug/mL | |
|
| 4 | 23.126 | 298301 | 6765 | 20.0000 | ug/mL | Guanine |
|
| 5 | 27.392 | 324193 | 6323 | 20.0000 | ug/mL | Hypoxanthine nucleotide | |
| Total of | 1381794 | 44505 |
(3) Standard solutions of different concentrations of cytosine, adenine, uracil, guanine and inosinic acid were input to obtain five retention times and corresponding peak areas, as shown in table 3 and fig. 4:
TABLE 3
| Peak number | Retention time | Area of | Height | Concentration of | Concentration unit | Name of |
|
| 1 | 4.309 | 199639 | 19643 | 29.8925 | ug/ | Cytosine nucleotide | |
| 2 | 18.022 | 608547 | 17183 | 29.8906 | ug/ | Adenine nucleotide | |
| 3 | 19.199 | 292353 | 7898 | 29.9150 | ug/mL | |
|
| 4 | 23.116 | 432525 | 9594 | 29.8895 | ug/mL | Guanine |
|
| 5 | 27.380 | 469694 | 8943 | 29.8835 | ug/mL | Hypoxanthine nucleotide | |
| Total of | 2002758 | 63261 |
(4) Standard solutions of different concentrations of cytosine, adenine, uracil, guanine and inosinic acid were input to obtain five retention times and corresponding peak areas, as shown in table 4 and fig. 5:
TABLE 4
| Peak number | Retention time | Area of | Height | Concentration of | Concentration unit | Name of |
|
| 1 | 4.306 | 267922 | 26123 | 40.1799 | ug/ | Cytosine nucleotide | |
| 2 | 18.010 | 817228 | 22963 | 40.1822 | ug/ | Adenine nucleotide | |
| 3 | 19.182 | 394642 | 10564 | 40.2387 | ug/mL | |
|
| 4 | 23.095 | 585972 | 12845 | 40.2750 | ug/mL | Guanine |
|
| 5 | 27.349 | 636183 | 12012 | 40.2703 | ug/mL | Hypoxanthine nucleotide | |
| Total of | 2701946 | 84506 |
(5) Standard solutions of different concentrations of cytosine, adenine, uracil, guanine and inosinic acid were input to obtain five retention times and corresponding peak areas, as shown in table 5 and fig. 6:
TABLE 5
| Peak number | Retention time | Area of | Height | Concentration of | Concentration unit | Name of |
|
| 1 | 4.305 | 368754 | 35763 | 52.0538 | ug/ | Cytosine nucleotide | |
| 2 | 18.024 | 1126225 | 31614 | 52.0717 | ug/ | Adenine nucleotide | |
| 3 | 19.181 | 545619 | 14490 | 52.1247 | ug/mL | |
|
| 4 | 23.088 | 806031 | 17545 | 52.0436 | ug/mL | Guanine |
|
| 5 | 27.334 | 878346 | 16505 | 52.1102 | ug/mL | Hypoxanthine nucleotide | |
| Total of | 3724975 | 115917 |
(6) Standard curves of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and inosinic acid were respectively established by an external standard method, as shown in fig. 7 to fig. 9.
2. Detection of dietary supplement product samples based on rapid nucleotide nutritional supplementation
On the basis of the standard curve, the method for detecting the nucleotide in the dietary supplement product based on the nucleotide rapid nutrition supplement comprises the following steps:
the method comprises the following steps: taking a food supplement product sample 1 and a sample 2 which are frozen at minus 60 ℃ and based on nucleotide rapid nutrition supplement, unfreezing at normal temperature, taking a proper amount of sample in a 10mLEP tube, putting the tube into a high-speed centrifuge, centrifuging for 10min at 10000r/min, filtering with a 0.22um microporous filter membrane, putting the tube into a 1.5mL automatic sample feeding bottle, putting the bottle into a 4 ℃ refrigerator for storage, and taking the bottle out before analysis;
step four, respectively supplying the sample 1 and the sample 2 to the high performance liquid chromatography, and controlling the working parameters of the high performance liquid chromatography to be: a chromatographic column: ZORBAXeclipsePlusC18(4.6 x 250mm 5-Micron); the detection wavelength is as follows: 254 nm; column temperature: 30 ℃; flow rate: 0.5 ml/min; sample introduction amount: 10 mu l of the mixture; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with the volume ratio of 95: 5; isocratic elution; the nucleotide concentrations were obtained as shown in FIGS. 10 and 11. As can be seen from the figure, the concentrations of each substance in the dietary supplement product sample 1 were: 4.3244ug/mL of cytosine nucleotide, 1.8689ug/mL of adenine nucleotide, 7.7716ug/mL of uracil nucleotide, 32.5026 ug/mL of guanosine and 53.4535ug/mL of inosinic acid; the concentrations of each substance in dietary supplement product sample 1 were: cytosine nucleotide 0, adenine nucleotide 0, uracil nucleotide 0, guanine nucleotide 74.2496ug/mL and inosinic nucleotide 57.2852 ug/mL.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. The method for detecting the nucleotides in the dietary supplement product based on the rapid nucleotide nutrition supplement is characterized in that the nucleotides are five nucleotides of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and hypoxanthine adenine nucleotide; the detection method comprises the following steps:
the method comprises the following steps: respectively supplying standard solutions containing five nucleotides of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and hypoxanthine adenine nucleotide into the high performance liquid chromatography, and respectively establishing standard curves of different nucleotides by taking the concentration ratio of single nucleotide as an X axis and the peak area of the single nucleotide as a Y axis;
step two, taking a food supplement product sample frozen at minus 60 ℃ and based on nucleotide rapid nutrition supplement, unfreezing at normal temperature, taking a proper amount of sample in a 10mLEP tube, putting the tube into a high-speed centrifuge for 10min at 10000r/min, putting the tube into a 1.5mL automatic sample feeding bottle after passing through a 0.22um microporous filter membrane, putting the bottle into a 4 ℃ refrigerator for storage, and taking the bottle out before analysis;
and step three, supplying the sample to high performance liquid chromatography to obtain the concentration of the nucleotide.
2. The detection method according to claim 1, wherein in the first step, the operating parameters of the high performance liquid chromatography are: a chromatographic column: ZORBAXeclipsePlusC18(4.6 x 250mm 5-Micron); the detection wavelength is as follows: 254 nm; column temperature: 30 ℃; flow rate: 0.5 ml/min; sample introduction amount: 10 mu l of the mixture; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with the volume ratio of 95: 5; isocratic elution.
3. The detection method according to claim 1, wherein in the second step, the operating parameters of the high performance liquid chromatography are: a chromatographic column: ZORBAXeclipsePlusC18(4.6 x 250mm 5-Micron); the detection wavelength is as follows: 254 nm; column temperature: 30 ℃; flow rate: 0.5 ml/min; sample introduction amount: 10 mu l of the mixture; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with the volume ratio of 95: 5; isocratic elution.
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| PCT/CN2022/099207 WO2023273903A1 (en) | 2021-07-01 | 2022-06-16 | Dietetic invigoration product capable of rapidly supplementing nutrients by nucleotide and preparation method therefor, and detection method for nucleotide in dietetic invigoration product |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023273903A1 (en) * | 2021-07-01 | 2023-01-05 | 百珍堂生物科技(浙江)有限公司 | Dietetic invigoration product capable of rapidly supplementing nutrients by nucleotide and preparation method therefor, and detection method for nucleotide in dietetic invigoration product |
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