CN113552153A - Strontium finlet marking method for non-lethal freshwater fish and detection method thereof - Google Patents
Strontium finlet marking method for non-lethal freshwater fish and detection method thereof Download PDFInfo
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 87
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 title claims abstract description 83
- 229910052712 strontium Inorganic materials 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 51
- 231100001160 nonlethal Toxicity 0.000 title claims abstract description 34
- 239000013505 freshwater Substances 0.000 title claims abstract description 30
- 238000001514 detection method Methods 0.000 title abstract description 19
- 239000000523 sample Substances 0.000 claims abstract description 14
- 238000004140 cleaning Methods 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000005498 polishing Methods 0.000 claims abstract description 6
- 229920005989 resin Polymers 0.000 claims description 19
- 239000011347 resin Substances 0.000 claims description 19
- 239000003292 glue Substances 0.000 claims description 12
- 239000008399 tap water Substances 0.000 claims description 8
- 235000020679 tap water Nutrition 0.000 claims description 8
- VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 239000003822 epoxy resin Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 229920000647 polyepoxide Polymers 0.000 claims description 5
- 229940047908 strontium chloride hexahydrate Drugs 0.000 claims description 5
- AMGRXJSJSONEEG-UHFFFAOYSA-L strontium dichloride hexahydrate Chemical compound O.O.O.O.O.O.Cl[Sr]Cl AMGRXJSJSONEEG-UHFFFAOYSA-L 0.000 claims description 5
- 229910001427 strontium ion Inorganic materials 0.000 claims description 5
- 230000000366 juvenile effect Effects 0.000 claims description 3
- 230000001418 larval effect Effects 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 2
- 230000002842 otolith Effects 0.000 description 14
- 210000001265 otolithic membrane Anatomy 0.000 description 14
- 241001275890 Megalobrama amblycephala Species 0.000 description 10
- 241000356847 Otolithes Species 0.000 description 8
- 210000004690 animal fin Anatomy 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 239000003550 marker Substances 0.000 description 6
- 238000005273 aeration Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 229960001124 trientine Drugs 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 3
- 210000000006 pectoral fin Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000003578 releasing effect Effects 0.000 description 2
- 241000883303 Acipenser sinensis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700608 Sagitta Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000003027 ear inner Anatomy 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
- G01N23/2202—Preparing specimens therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
- A01K61/95—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination specially adapted for fish
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
- G01N23/225—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
- G01N23/2251—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion using incident electron beams, e.g. scanning electron microscopy [SEM]
- G01N23/2252—Measuring emitted X-rays, e.g. electron probe microanalysis [EPMA]
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/07—Investigating materials by wave or particle radiation secondary emission
- G01N2223/079—Investigating materials by wave or particle radiation secondary emission incident electron beam and measuring excited X-rays
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/10—Different kinds of radiation or particles
- G01N2223/102—Different kinds of radiation or particles beta or electrons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/60—Specific applications or type of materials
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- G01N2223/6126—Specific applications or type of materials biological material tissue
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Abstract
The invention discloses a method for marking strontium finny of non-lethal freshwater fish, which comprises the following steps: step a: preparing a strontium marking solution in a marking container; step b: moving the fish to be marked into a marking solution in a marking container, and exposing for a short time to deposit strontium elements on fin rays of the marked fish; step c: after exposure is complete, the fish are removed from the marked container. The invention also discloses a detection method of the non-lethal freshwater fish fin-ray strontium marking method, which comprises the following steps: step a: catching a fish to be detected and picking up the fin rays; step b: cleaning, drying, embedding, slicing and polishing the fin rays, ultrasonically cleaning the fin rays and drying; step c: and (3) analyzing the strontium content of the fin rays by using an electronic probe micro-area analyzer, and determining whether the fish is marked according to whether the analyzed result has high strontium mark characteristics, wherein the fish is marked if the high strontium mark is contained, and the fish is not marked if the high strontium mark is not contained.
Description
Technical Field
The invention belongs to the technical field of evaluation and protection of aquatic wild animal resources, and particularly relates to a method for marking strontium on fin-shaped rays of non-lethal freshwater fishes and a detection method thereof.
Background
The proliferation and releasing work is an important national policy in China, has obtained better economic, social and ecological benefits, and plays an important role in recovering aquatic organism resources in certain key water areas or excellent varieties. Although the proliferation and release work shows a lot of advantages, the existing method still has the limitations that an effect evaluation system is incomplete, a necessary and objective evaluation technology system is not built, and the like. The above limitations are mainly caused by the lack of a convenient, effective and objective release biomarker technique. Therefore, a labeling technology and a detection technology which can reflect the releasing effect in a long-acting and objective manner and are non-lethal and low-damage to aquatic organisms (especially some rare fishes such as Acipenser sinensis and the like) are needed.
At present, a lot of fish marking technologies exist, wherein otolith microchemical (strontium) marking is used as an emerging marking technology to be applied to more and more released fish marking work due to the advantages that the marking work is simple to operate, large-batch simultaneous marking can be carried out, the marking has almost no influence on fish bodies, and the marking is carried for life once being marked. However, since otoliths are tissues in the inner ear of a fish body, there are 3 pairs, and sagitta (which is a common structural cause of cyprinid fishes, and is mostly star or micro otoliths) is a subject to be studied, it is necessary to die the fish body at the time of sampling, and there is a great limit in the number of samples. Furthermore, since the otolith grows in a spherical envelope, this results in a complex, time-consuming and very delicate grinding process for the analysis of the anterior otolith (the otolith core must be exposed), high demands on the operator and no possibility of batch operation. In addition, due to the area of the cross section, the otoliths must be analyzed one by one during analysis and cannot be rapidly detected in batches.
Therefore, it is of no doubt very important to find a otolith substitute material which can be obtained quickly (even non-lethal), processed in batches and analyzed in batches for relevant marking work. Based on these objective conditions, we are forced to find a convenient, fast, simple, efficient, objective, but non-lethal, low-injury marking technique.
Disclosure of Invention
In order to solve the technical problem that the death of fish bodies must be caused when sampling is carried out after the marking is finished by the conventional fish marking technology, the invention provides the strontium finlet marker for the non-lethal freshwater fish and the detection method, and the marking and detection technology for the freshwater fish can be conveniently, quickly, simply, efficiently and objectively carried out under the conditions of non-lethal and low-damage.
The invention adopts the following technical scheme:
a strontium finnwell marking method for non-lethal freshwater fishes comprises the following steps:
step a: a strontium marking solution is arranged in a marking container, wherein the concentration of strontium ions is 600-1000 mg/L;
step b: moving the fish to be marked into a marking solution in a marking container, and exposing for a short time to deposit strontium elements on fin rays of the marked fish;
step c: after exposure is complete, the fish are removed from the marked container.
Further, in the step a, the material of the marking container is glass or plastic.
Further, in the step a, strontium chloride hexahydrate is selected when the strontium marking solution is prepared.
Further, in the step b, the fish to be marked is in a larval stage or a juvenile stage; the short-term exposure time is 3-7 days.
Further, in the step c, after the exposed fish is moved out of the marking container, the fish is rinsed for 3-5 times by using aerated tap water without the pollution of the marking solution.
A detection method of the strontium finnwell of the non-lethal freshwater fish marked by the marking method comprises the following steps:
step a: catching a fish to be detected and picking up the fin rays;
step b: cleaning, drying, embedding, slicing and polishing the fin rays, ultrasonically cleaning the fin rays and drying;
step c: and (3) analyzing the strontium content of the fin rays by using an electronic probe micro-area analyzer, and determining whether the fish is marked according to whether the analyzed result has high strontium mark characteristics, wherein the fish is marked if the high strontium mark is contained, and the fish is not marked if the high strontium mark is not contained.
Further, in the step a, the position where the fin ray is extracted is the fin ray base.
Further, in step b, the embedding comprises the following steps:
step ba: sticking 10-20 fin rays on a solidified resin sheet with the thickness of 3-8 mm by glue in a lying mode;
step bb: the whole fin and the resin sheet are horizontally laid and stuck to the bottom of the mold by glue;
step bc: and after the glue is solidified, pouring a mixed reagent with the weight ratio of epoxy resin to triethylenetetramine being 7g: 1-2 g for embedding.
Further, in the step b, the slice thickness is 1-3 mm.
Further, in the step c, the conditions when the electron probe micro-area analyzer performs the strontium content analysis of the fin-shaped ray are as follows: voltage 15 kV, current 3~ 4X 10-7A, the diameter of the beam spot is 2-3 μm, and the strontium content is 0-0.2% to 0.5-0.8% corresponding to the color scale.
Compared with the prior art, the invention has the following beneficial effects:
(1) in the prior art, otoliths are used as marking objects, target fishes need to be killed to obtain samples when sampling is carried out, and the marking method provided by the invention uses fish fins as marking objects, so that the fishes do not need to be killed when sampling, and the non-lethal purpose is achieved.
(2) For fishes, the number of the otoliths is only 2, so that the loss is easily caused during the test, the subsequent test requirements are influenced, and when the fins are selected as the marked objects, the number of the fins is large, so that the loss of precious samples is avoided.
(3) The removal of the fin rays can also effectively avoid the damage to the external form of the commercial fish in the sample removal process, and the method has more advantages in later popularization and application.
Drawings
Fig. 1 is a strontium-labeled diagram of the back fin of the megalobrama amblycephala in example 1.
Fig. 2 is a graph of the marker of the ventral fin of megalobrama amblycephala in example 2.
Fig. 3 is a marker chart of pectoral fin and tail fin of megalobrama amblycephala in example 3.
Fig. 4 is a pectoral fin image of a comparative megalobrama amblycephala.
Detailed Description
The present invention is further described with reference to the following examples, which are provided for illustration only and are not to be construed as limiting the scope of the claims, and other alternatives which may occur to those skilled in the art are also within the scope of the claims.
Example 1
A strontium finlet marking method for non-lethal freshwater fishes adopts the following process steps:
step a: preparing a strontium chloride hexahydrate marking solution in a pp-made culture tank, wherein the concentration of strontium ions is 800 mg/L;
step b: placing the young megalobrama amblycephala into a culture tank, and exposing for 5 days;
step c: after exposure, the fish were removed from the exposure vessel, rinsed 3 times with aerated tap water without contamination by the marker solution, and then placed in a glass jar containing aerated tap water for farming.
The detection method of the fish fin rays marked by the non-lethal freshwater fish fin ray strontium marking method comprises the following steps:
step a: during detection, a section of dorsal fin is picked up close to the base;
step b: after the back fins are cleaned and dried, firstly, 10 fin rays are stuck on a solidified resin sheet with the thickness of 5mm by AB glue in a lying mode, then, the whole fin ray resin block is stuck at the bottom of a mould in a lying mode by the AB glue, and after the resin is solidified, epoxy resin and triethylene tetramine uniformly-mixed reagent (the weight ratio is 7g:1 g) is poured for embedding. After the resin is solidified, cutting a 1mm fin-shaped resin sheet, polishing, cleaning and airing for later use;
step c: using an electron probe micro-area analyzer to analyze the strontium content on the cross section, wherein the distribution analysis conditions of the strontium content surface of the electron probe are as follows: 15 kV, 3.6X 10-7A, the diameter of the beam spot is 3 μm, and the Sr content corresponds to that of the beam spotThe color scale range is 0.2% -0.6%.
As shown in fig. 1, the detection result of this embodiment shows that there is an obvious strontium-labeled ring, i.e., a strontium-labeled feature, on the back fin of a megalobrama amblycephala, which proves that the strontium-labeled method for the fin of a non-lethal freshwater fish used in this embodiment effectively labels strontium on the back fin of the fish.
Example 2
A strontium finlet marking method for non-lethal freshwater fishes adopts the following process steps:
step a: preparing a strontium chloride hexahydrate marking solution in a pp-made culture tank, wherein the concentration of strontium ions is 800 mg/L;
step b: placing the young megalobrama amblycephala into a culture tank, and exposing for 5 days;
step c: after exposure, the fish is moved out of the exposure container, rinsed by 3 times of aeration tap water without being polluted by the marking solution, and then put into a glass jar containing the aeration tap water for cultivation;
the detection method of the fish fin rays marked by the non-lethal freshwater fish fin ray strontium marking method comprises the following steps:
step a: during detection, a section of ventral fin is picked up close to the base;
step b: after the ventral fins are cleaned and dried, firstly, 15 fin rays are stuck on a solidified resin sheet with the thickness of 5mm by AB glue in a lying mode, then, the whole fin ray resin block is stuck at the bottom of a mould by the AB glue in a lying mode, and after the resin is solidified, epoxy resin and triethylene tetramine uniformly-mixed reagent (the weight ratio is 7g:1 g) is poured for embedding. After the resin is solidified, cutting a 1mm fin-shaped resin sheet, polishing, cleaning and airing for later use;
step c: using an electron probe micro-area analyzer to analyze the strontium content on the cross section, wherein the distribution analysis conditions of the strontium content surface of the electron probe are as follows: 15 kV, 3.6X 10-7A, the diameter of the beam spot is 2 μm, and the Sr content is 0.2-0.6% corresponding to the color scale.
As shown in fig. 2, the detection result of this embodiment shows that there is an obvious strontium-labeled ring, i.e., a strontium-labeled feature, on the fin-line of the megalobrama amblycephala, which proves that the strontium-labeled method for the fin-line of the non-lethal freshwater fish used in this embodiment effectively labels strontium on the abdominal fin of the fish.
Example 3
A method for marking and detecting non-lethal strontium finlet of freshwater fish comprises the following steps:
step a: preparing a strontium chloride hexahydrate marking solution in a pp-made culture tank, wherein the concentration of strontium ions is 800 mg/L;
step b: placing the young megalobrama amblycephala into a culture tank, and exposing for 5 days;
step c: after exposure, the fish is moved out of the exposure container, rinsed by 3 times of aeration tap water without being polluted by the marking solution, and then put into a glass jar containing the aeration tap water for cultivation;
the detection method of the fish fin rays marked by the non-lethal freshwater fish fin ray strontium marking method comprises the following steps:
step a: during detection, a section of chest fin strip and tail fin strip are picked up close to the base;
step b: after the ventral fins are cleaned and dried, 10 sets of chest fin rays and tail fin rays are firstly stuck on a solidified resin sheet with the thickness of 3mm by AB glue in a lying mode, then the whole fin ray resin block is stuck on the bottom of a mould in a lying mode by the AB glue, and after the resin is solidified, epoxy resin and triethylene tetramine uniformly mixed reagent (the weight ratio is 7g:1 g) is poured for embedding. After the resin is solidified, cutting a 1mm fin-shaped resin sheet, polishing, cleaning and airing for later use;
step c: using an electron probe micro-area analyzer to analyze the strontium content on the cross section, wherein the distribution analysis conditions of the strontium content surface of the electron probe are as follows: 15 kV, 3.6X 10-7A, the diameter of the beam spot is 2 μm, and the Sr content is 0.2-0.6% corresponding to the color scale.
As shown in fig. 3, the detection result of this embodiment shows that there are obvious strontium-labeled rings, i.e., strontium-labeled features, on the pectoral fin-line and the tail fin-line of megalobrama amblycephala, which proves that the strontium-labeled method for the fin-line of the non-lethal freshwater fish used in this embodiment effectively labels strontium on the pectoral fin and the tail fin of the fish.
Comparative example
A method for marking and detecting non-lethal strontium finny of freshwater fish, which is basically the same as the example 3, except that the strontium concentration of the strontium marking solution prepared in the step a is 500 mg/L.
The detection method of the fin rays of the fish marked by the non-lethal freshwater fish fin ray strontium marking method is basically the same as that of the embodiment 3, and the difference is that the chest fin ray is selected for testing.
As shown in fig. 4, the test results of the comparative example, the strontium-labeled ring was not detected because the concentration of strontium in the strontium-labeled solution affected the deposition of strontium on fish, and it was difficult to deposit strontium on fish fins when the concentration of strontium was too low. The existing strontium marks are marked on the otolith.
In conclusion, the strontium marking method provided by the invention deposits strontium element on the fins through short-term exposure, and obvious strontium rings are obtained when an electronic probe micro-area analyzer is used for detection, which indicates that strontium marking is successfully carried out on the fins. In the prior art, the otolith is used for marking, and compared with the otolith in the fish brain, the removal of one fin ray can effectively avoid the death of the fish body; the two pairs of the fish fins have a plurality of fins and only two corresponding usable otoliths, so that the fin can avoid the loss of precious samples caused by the treatment process; the three-fin-shaped strip can effectively avoid the damage to the external form of the commercial fish in the sample picking process, and has more advantages in later-stage popularization and application.
For the determination of the protection range of the invention, the fish is developed into larval fish, juvenile fish, adult fish and other stages, and the individual of different fishes also has the difference in size. From past experience, individual conditions corresponding to an earlier life history need to be reduced appropriately, whereas conditions need to be increased appropriately. The fish size also has the above-mentioned influence. Thus, the concentration of the strontium marker solution in the examples was 800mg/L, the time and concentration ranges were set for the respective exposure conditions to 600-1000 mg/L, and the comparative example demonstrated that too low a concentration could not be deposited on the fin-lines, while the upper limit was set on the one hand to be low enough that no waste was necessary, and on the other hand the marker was essentially "competitive" with chemically similar elements deposited on the fin-lines, so that too high a concentration had some effect on the fish.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A method for marking strontium on fin-shaped rays of non-lethal freshwater fishes is characterized by comprising the following steps: the method comprises the following steps:
step a: a strontium marking solution is arranged in a marking container, wherein the concentration of strontium ions is 600-1000 mg/L;
step b: moving the fish to be marked into a marking solution in a marking container, and exposing for a short time to deposit strontium elements on fin rays of the marked fish;
step c: after exposure is complete, the fish are removed from the marked container.
2. The method for marking strontium finlet of non-lethal freshwater fish according to claim 1, wherein the method comprises the following steps: in the step a, the marking container is made of glass or plastic.
3. The method for marking strontium finlet of non-lethal freshwater fish according to claim 1, wherein the method comprises the following steps: in the step a, strontium chloride hexahydrate is selected when preparing the strontium marking solution.
4. The method for marking strontium finlet of non-lethal freshwater fish according to claim 1, wherein the method comprises the following steps: in the step b, the fish to be marked is in a larval stage or a juvenile stage; the short-term exposure time is 3-7 days.
5. The method for marking strontium finlet of non-lethal freshwater fish according to claim 1, wherein the method comprises the following steps: and c, after the exposed fish is moved out of the marking container, rinsing the fish for 3-5 times by using aerated tap water without the pollution of the marking solution.
6. A method for detecting strontium finns of non-lethal freshwater fishes marked by the marking method according to any one of claims 1 to 5, which comprises the following steps: the method comprises the following steps:
step a: catching a fish to be detected and picking up the fin rays;
step b: cleaning, drying, embedding, slicing and polishing the fin rays, ultrasonically cleaning the fin rays and drying;
step c: and (3) analyzing the strontium content of the fin rays by using an electronic probe micro-area analyzer, and determining whether the fish is marked according to whether the analyzed result has high strontium mark characteristics, wherein the fish is marked if the high strontium mark is contained, and the fish is not marked if the high strontium mark is not contained.
7. The method for detecting strontium finlet of non-lethal freshwater fish according to claim 6, wherein the method comprises the following steps: in the step a, the position where the fin ray is picked is the base of the fin ray.
8. The method for detecting strontium finlet of non-lethal freshwater fish according to claim 6, wherein the method comprises the following steps: in step b, the embedding comprises the following steps:
step ba: sticking 10-20 fin rays on a solidified resin sheet with the thickness of 3-8 mm by glue in a lying mode;
step bb: the whole fin and the resin sheet are horizontally laid and stuck to the bottom of the mold by glue;
step bc: and after the glue is solidified, pouring a mixed reagent with the weight ratio of epoxy resin to triethylenetetramine being 7g: 1-2 g for embedding.
9. The method for detecting strontium finlet of non-lethal freshwater fish according to claim 6, wherein the method comprises the following steps: in the step b, the slice thickness is 1-3 mm.
10. The method for detecting strontium finlet of non-lethal freshwater fish according to claim 6, wherein the method comprises the following steps: in the step c, the conditions of the electron probe micro-area analyzer for analyzing the strontium content of the fin ray are as follows: voltage 15 kV, current 3~ 4X 10-7A, beam spot diameter 2-3 μm, strontiumThe content is 0-0.2% to 0.5-0.8% corresponding to the color scale.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110849173.0A CN113552153A (en) | 2021-07-27 | 2021-07-27 | Strontium finlet marking method for non-lethal freshwater fish and detection method thereof |
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| CN202110849173.0A CN113552153A (en) | 2021-07-27 | 2021-07-27 | Strontium finlet marking method for non-lethal freshwater fish and detection method thereof |
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| CN107258647A (en) * | 2017-05-24 | 2017-10-20 | 中国水产科学研究院黄海水产研究所 | A kind of lefteye flounder fish otolith rubidium marking method |
| CN109738266A (en) * | 2018-12-19 | 2019-05-10 | 中国水产科学研究院淡水渔业研究中心 | A kind of pre-treating method of mandarin sturgeon pectoral fin micro-chemical analysis |
| CN110463644A (en) * | 2019-08-20 | 2019-11-19 | 中国水产科学研究院东海水产研究所 | A kind of otolith rubidium marking method of black porgy enhancement releasing fry |
| CN110672648A (en) * | 2019-10-28 | 2020-01-10 | 中国水产科学研究院淡水渔业研究中心 | Method for distinguishing different resource groups of non-lethal coilia ectenes |
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| CN104106481A (en) * | 2014-06-23 | 2014-10-22 | 中国水产科学研究院东海水产研究所 | Marking method for proliferation and releasing of large yellow croakers |
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| CN110672648A (en) * | 2019-10-28 | 2020-01-10 | 中国水产科学研究院淡水渔业研究中心 | Method for distinguishing different resource groups of non-lethal coilia ectenes |
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