CN107258647A - A kind of lefteye flounder fish otolith rubidium marking method - Google Patents
A kind of lefteye flounder fish otolith rubidium marking method Download PDFInfo
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- CN107258647A CN107258647A CN201710373278.7A CN201710373278A CN107258647A CN 107258647 A CN107258647 A CN 107258647A CN 201710373278 A CN201710373278 A CN 201710373278A CN 107258647 A CN107258647 A CN 107258647A
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- otolith
- strontium
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- lefteye flounder
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- 230000002842 otolith Effects 0.000 title claims abstract description 63
- 210000001265 otolithic membrane Anatomy 0.000 title claims abstract description 63
- 241000356847 Otolithes Species 0.000 title claims abstract description 61
- 241000694873 Paralichthyidae Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 15
- 229910052701 rubidium Inorganic materials 0.000 title claims abstract description 12
- IGLNJRXAVVLDKE-UHFFFAOYSA-N rubidium atom Chemical compound [Rb] IGLNJRXAVVLDKE-UHFFFAOYSA-N 0.000 title claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 229910052712 strontium Inorganic materials 0.000 claims abstract description 41
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 claims abstract description 40
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical class [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 claims abstract description 18
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 14
- 239000004467 fishmeal Substances 0.000 claims abstract description 14
- 238000005660 chlorination reaction Methods 0.000 claims abstract description 12
- 239000008188 pellet Substances 0.000 claims description 20
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 229910001631 strontium chloride Inorganic materials 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims 1
- 241000251468 Actinopterygii Species 0.000 abstract description 21
- 238000002372 labelling Methods 0.000 abstract description 13
- 230000015572 biosynthetic process Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 230000002688 persistence Effects 0.000 abstract description 3
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical group [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 230000000366 juvenile effect Effects 0.000 description 5
- 241000269979 Paralichthys olivaceus Species 0.000 description 4
- 238000005498 polishing Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000000151 deposition Methods 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 238000004453 electron probe microanalysis Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 235000010216 calcium carbonate Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000010894 electron beam technology Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000269981 Bothidae Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 244000137852 Petrea volubilis Species 0.000 description 1
- 241000269908 Platichthys flesus Species 0.000 description 1
- 241000700608 Sagitta Species 0.000 description 1
- 229910002370 SrTiO3 Inorganic materials 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- PWIGYBONXWGOQE-UHFFFAOYSA-N alizarin complexone Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C=C(CN(CC(O)=O)CC(=O)O)C(O)=C2O PWIGYBONXWGOQE-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 210000003027 ear inner Anatomy 0.000 description 1
- 229910001651 emery Inorganic materials 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 210000005224 forefinger Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 239000004848 polyfunctional curative Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005211 surface analysis Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000001771 vacuum deposition Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
- A01K61/95—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination specially adapted for fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The present invention provides a kind of lefteye flounder fish otolith rubidium marking method, is characterized in：Comprise the following steps：（1）Six water strontium chlorides are well mixed with fish meal, make to contain strontium element in fish meal, obtain marking feed；（2）Use mark feedstuff feeding lefteye flounder fish to be marked；（3）Mark feed is fed daily 12 times, Feeding time is at least 3 days；（4）Six water chlorination content of strontium at least account for the 0.8% of mark feed gross mass.Strontium element can be deposited on otolith growth ring by physiology courses such as blood-transmitteds, formation element annulus, and generation releases the effect of lefteye flounder otolith elemental fingerprints mark.Otolith strontium element Finger-print labelling method stability is good, not by ectocine, can be with persistence.Six water strontium chlorides are harmless to fish to be marked, and high-concentration and low-concentration fatal rate is very low.
Description
Technical field
The invention belongs to release marker technique field, it is related to the labeling method of lefteye flounder fish, specifically a kind of lefteye flounder fish ear
Stone rubidium marking method.
Background technology
Lefteye flounder is unique Bothidae lefteye flounder fish of Asia bank distribution, because its individual is big, fast growth, and delicious flavour is
The economic lefteye flounder fish of the important seawater in East Asia various countries.It is migrated apart from short, is the main product of East Asia various countries enhancement releasing seawater lefteye flounder fish
One of kind.China's lefteye flounder enhancement releasing is main in provinces and cities such as Shandong, Hebei, Liaoning and Tianjin, wherein 2016 are only Liaoning Province's tooth
Competitive bidding releases lefteye flounder quantity for 8,130,000 tails.Over nearly 20 years, biology, seed rearing and propagation are bred lefteye flounder by China
Numerous studies and work have been done in terms of releasing, rich experiences are have accumulated, but for enhancement releasing, its recruitment evaluation technology is extremely
It is modern not yet ripe.Wherein one of the main reasons is that the acquisition of information such as the how many, specification of lefteye flounder are released in catches is difficult.To releasing fish
Being marked can release to be distinguished in catches
Lefteye flounder provides strong foundation.
Otolith is that lefteye flounder fish inner ear natural biology mineralising deposits the calcium carbonate structure to be formed, and it is earliest hard to be that lefteye flounder fish is formed
Tissue, its fine structure can be for biological informations such as the ages of interpretation lefteye flounder fish, and deposits element information therein and then remember
The environmental information that lefteye flounder fish lived in the past is recorded.Chemical element in water environment enters blood by the tissue such as lefteye flounder fish gill portion
Liquid, the physiology course such as blood-transmitted is deposited on otolith growth ring, formation element annulus, i.e. elemental fingerprints（elemental
fingerprints）.The water environment that the existence of lefteye flounder fish is constituted in different elements, its otolith element composition is different, i.e. elemental fingerprints
It is different.Otolith elemental fingerprints mark can be to releasing seed all mark, and once marks not by ectocine, can be with persistence.
Otolith elemental fingerprints mark cost is low, and labeling method is simple and easy to operate, small to releasing seed damage.Therefore, otolith elemental fingerprints
Mark turns into one of focus of China's lefteye flounder fish releasing mark technical research.
Chinese patent（The patent No.：ZL201110172710.9）Disclosed labeling method is：Alizarin complexone, fish are used
Mixed feed is mixed, and mark feed is made, fish to be marked is then fed, recognizable so as to be detected on mark fish otolith
Fluorescence labeling.It avoids other labeling methods and injures larger to fish body, and mark loss rate is high, marks expensive, conservation rate
It is low, the shortcomings of marker number is limited.But the defect that labeling method is present in above-mentioned technology is, Tagging fish survival rate when concentration is higher
It is severely impacted, highest can make Tagging fish all dead.
The content of the invention
Above mentioned problem of the present invention to solve prior art presence is kept away there is provided a kind of lefteye flounder fish otolith rubidium marking method
The marker material for exempting to use leads the problem of fatal rate is high when concentration is higher.
The purpose of the present invention is achieved through the following technical solutions：
A kind of lefteye flounder fish otolith rubidium marking method, it is characterised in that comprise the following steps：
（1）Six water strontium chlorides are well mixed with fish meal, make to contain strontium element in fish meal, obtain marking feed；
（2）Use mark feedstuff feeding lefteye flounder fish to be marked；
（3）Mark feed is fed daily 1-2 times, Feeding time is at least 3 days；
（4）Six water chlorination content of strontium at least account for the 0.8% of mark feed gross mass.
Improvement to above-mentioned technical proposal：The step（4）In, the six water chlorination content of strontium accounts for mark feed gross mass
1%-10%.
Further improvement to above-mentioned technical proposal：The step（3）In, mark feed is fed daily 2 times, Feeding time
For 4-10 days.
Further improvement to above-mentioned technical proposal：The step（1）In, the fish meal is pellet, with biology
Phycocolloid is adhesive, and 1kg pellets, will at least biological phycocolloid of the water strontium chlorides of 8g six and 10-15g using the biological phycocolloid of 10-15g
100ml pure water is dissolved in, watering can is poured into after being well mixed, is uniformly sprayed on 1kg pellets, room temperature 24h dries.
Present invention advantage compared with prior art and good effect are：
The present invention can be deposited on otolith growth ring using mark feed method, strontium element is fed by physiology courses such as blood-transmitteds
On, formation element annulus, generation releases the effect of lefteye flounder otolith elemental fingerprints mark.Otolith strontium element Finger-print labelling method stability is good,
, can be with persistence not by ectocine.Six water strontium chlorides are harmless to fish to be marked, and high-concentration and low-concentration fatal rate is very low.
Brief description of the drawings
Fig. 1 is that different gradient groups recover the quantitative line analysis figure of 30 days lefteye flounder otoliths；
Fig. 2 is that different gradient group lefteye flounder otoliths recover 30 days strontium deposition conditions mappings.
Embodiment
The present invention is described in further detail below：
A kind of embodiment of lefteye flounder fish otolith rubidium marking method of the present invention, comprises the following steps：
（1）Six water strontium chlorides are well mixed with fish meal, make to contain strontium element in fish meal, obtain marking feed；
（2）Use mark feedstuff feeding lefteye flounder fish to be marked；
（3）Mark feed is fed daily 1-2 times, Feeding time is at least 3 days；
（4）Six water chlorination content of strontium at least account for the 0.8% of mark feed gross mass.
It is preferred that, the step（3）In, mark feed is fed daily 2 times, Feeding time is 4-10 days;The step（4）
In, the six water chlorination content of strontium accounts for the 1%-10% of mark feed gross mass.
It is below 3 specific embodiments of the present invention：
Embodiment 1：
（1）Make mark feed：Fish meal selects pellet, using biological phycocolloid as adhesive, and 1kg pellets use 10-
15g biology phycocolloid.The water strontium chlorides of 8g six and the biological phycocolloid of 10-15g are dissolved in 100ml pure water, watering can is poured into after being well mixed,
Even to be sprayed on 1kg pellets, room temperature 24h dries.Experiment is eastern ball feed with pellet, and specification is C0-C3；
（2）Using mark feedstuff feeding lefteye flounder fish to be marked, and ensure without residual bait；
（3）8 points of every morning and 2 pm respectively feed 1 mark feed and (or 1 mark feeding are only fed in 6 points of every morning
Material), single feeding volume is fed by the 2%-3% of fish body gross weight, and Feeding time is 10 days；Test the initial average weight of lefteye flounder fish
2.64g, recovers 30 days average weight 10.89g；
（4）Six water chlorination content of strontium account for the 0.8% of mark feed gross mass；
（5）Prevent strontium chloride from diffusing to breeding water body using biological phycocolloid as adhesive, every morning feeds end and discharged water clearly
After groove, add water and keep 24h flowing water, flow velocity is circulation in 30 minutes.
Embodiment 2：
（1）Make mark feed：Fish meal selects pellet, using biological phycocolloid as adhesive, and 1kg pellets use 10-
15g biology phycocolloid.The water strontium chlorides of 32g six and the biological phycocolloid of 10-15g are dissolved in 100ml pure water, watering can is poured into after being well mixed,
Uniformly it is sprayed on 1kg pellets, room temperature 24h dries.Experiment is eastern ball feed with pellet, and specification is C0-C3；
（2）Using mark feedstuff feeding lefteye flounder fish to be marked, and ensure without residual bait；
（3）8 points of every morning and 2 pm respectively feed 1 mark feed daily.Single feeding volume is thrown by fish body gross weight 2%-3%
Hello, Feeding time is 3 days；The initial average weight 2.51g of lefteye flounder fish is tested, recovers 30 days average weight 10.01g；
（4）Six water chlorination content of strontium account for the 3.2% of mark feed gross mass；
（5）Prevent strontium chloride from diffusing to breeding water body using biological phycocolloid as adhesive, every morning feeds end and discharged water clearly
After groove, add water and keep 24h flowing water, flow velocity is circulation in 30 minutes.
Embodiment 3：
（1）Make mark feed：Fish meal selects pellet, using biological phycocolloid as adhesive, and 1kg pellets use 10-
15g biology phycocolloid.The water strontium chlorides of 64g six and the biological phycocolloid of 10-15g are dissolved in 100ml pure water, watering can is poured into after being well mixed,
Uniformly it is sprayed on 1kg pellets, room temperature 24h dries.Experiment is eastern ball feed with pellet, and specification is C0-C3；
（2）Using mark feedstuff feeding lefteye flounder fish to be marked, and ensure without residual bait；
（3）8 points of every morning and 2 pm respectively feed 1 mark feed daily.Single feeding volume presses the 2%-3% of fish body gross weight
Feed, Feeding time is 10 days.The initial average weight 2.58g of lefteye flounder fish is tested, recovers 30 days average weight 10.02g；
（4）Six water chlorination content of strontium account for the 6.4% of mark feed gross mass；
（5）Prevent strontium chloride from diffusing to breeding water body using biological phycocolloid as adhesive, every morning feeds end and discharged water clearly
After groove, add water and keep 24h flowing water, flow velocity is circulation in 30 minutes.
In order to further prove the effect of the present invention, the applicant has done following experiment：
First, materials and methods
1st, experiment material and design
It is that daily output April 4 in 2016 fish-egg cultivates gained to test fish, and this is tested grinds on June 24th, 2016 in Chinese aquatic science
Study carefully institute Bei Dai River center experiment centre to proceed by, experiment fish culture is in 300L fiberglass circular channels, and 8 points of every morning is with
2 points of noon respectively feeds 1 time, experiment sets 0,1,8,4 strontiums mark feed gradients such as 64g/Kg（Six water strontium chloride g/ pellets Kg）,
Persisted moniker 10 days.Each gradient group experiment fish specification is consistent, and quantity is 400 tails.During mark, each gradient feeding volume is identical,
And ensure that each gradient is residual bait；Feed end and discharge water after clear groove in the morning, adds water and keeps 24h flowing water.Recorded during mark each
30d measurements record experiment fish growth traits and collecting sample after the gradient experiment fish death rate, mark, -20 DEG C of preservations.Each gradient group
It is each to choose 2 samples progress otolith micro-chemical analysis.
2nd, otolith collection and pre-treatment
The relatively uniform experiment fish collection otolith of size is chosen from the sample of collection, different recovery time each gradient groups take experiment fish
2 tails.After sagitta is taken out using instruments such as scissors, fine-pointed forcepses, cleaned in clear water, otolith is rejected with dissecting needle or thin bamboo stick
After the organic matter of surface attachment, then with alcohol washes, and in dissection Microscopic observation cleaning performance.48 holes are put into after cleaning up
Box, 38 DEG C of dry for standby.For increase comparativity, this experiment is unified to carry out elemental fingerprints analysis using bra vector otolith.
Otolith sample is placed on embedding box base, a droplet AB glue is put above base with thin bamboo stick, by the ear got ready
Stone is placed on AB glue, and pressing lightly on otolith makes otolith steadily be fixed on base.The otolith sample fixed is stored at room temperature 20 points
Zhong Hou, uses epoxy resin（Epofix, struers company；Rensin and hardener mass ratioes 7:1）Embedding.It is put into after embedding
Baking oven, 38 DEG C dry more than 12 hours.The embedded block solidified is placed on the AB of slide, pressing embedding of being exerted oneself with forefinger
Block, bubble in AB glue is extruded, and is fixed after 2 hours and is used the grinding mill of diamond-impregnated wheel（Discoplan-TS types, Struers
Company）Cutting is milled.Sample is through just after cutting（500 mesh emery wheels）, fine grinding（1200 mesh sand paper）The exposure of otolith core is polishing to, is used
Polished machine（Roto Pol-35, Struers companies of Denmark）Equip loom polishing disk and coordinate polishing fluid polishing, to otolith surface
Without obvious cut.Sample be put into Millo-Q water be cleaned by ultrasonic 5 minutes after, dry 24 hours, be completely dried under natural conditions
Afterwards, using vacuum coating equipment（JEE-420, Jeol Ltd.）Carbon film is deposited（36A, 25s）.
3rd, otolith elemental fingerprints analysis method
Use the JXA-8100 type electron probe microanalysers of Jeol Ltd.（EPMA）Carry out otolith Sr and Ca
Micro-chemical analysis.Straight line from otolith core along most major diameter to otolith edge carries out quantitative line analysis（line transect
analysis）.EPMA parameter setting：Accelerating potential is 15kV, and electron beam current is 2.0 × 10-8A；Beam spot diameter, is 2 μm,
Every residence time 15s；It is carried out continuously with 4 μm of spacing and gets measure ready.Standard sample uses calcium carbonate（CaCO3）And strontium titanates
（SrTiO3）.Surface analysis is carried out again after all otolith line analysises are complete（mapping analysis）.At this moment, EPMA accelerating potentials and
Electron beam current is respectively 15kV, and 5.0 × 10-7A, beam spot diameter, is 2 μm, and every residence time is 30ms, and pixel is 4 × 4 μ
m。
2nd, experimental result
1st, the Sr/Ca ratios of the different quantitative line analysises of gradient group lefteye flounder otolith
Fig. 1 show different gradient groups and recovers the quantitative line analysis contrast of 30d lefteye flounders otolith, as a result shows：0g/Kg, 1g/Kg gradient
The Sr/Ca values that group keeps relative stability, do not occur Sr/Ca values peak, illustrate that 1g/Kg gradients group does not form obvious strontium element
Finger-print labelling method.8g/Kg and 64g/Kg gradient compositions do not form 1-2 Sr/Ca value crest, illustrate 8g/Kg and 64g/Kg gradient groups
It has been respectively formed obvious strontium element Finger-print labelling method.
In Fig. 1, Fig. 2, BDHFE-1 is control group 1, and BDHFE-2 is control group 2, and BDHFE-3 is 1g/Kg-1 groups, BDHFE-
4 be 1g/Kg-2 groups, and BDHFE-5 is 8g/Kg-1 groups, and BDHFE-6 is 8g/Kg-2 groups, and BDHFE-7 is 64g/Kg-1 groups, BDHFE-
8 be 64g/Kg-2.
Further analysis learn, BDHFE-5 apart from 780-904 μm of otolith core at otolith section formed crest, most
High peak is 10.18；BDHFE-6 is in otolith section formation crest, highest crest at 780-956 μm of otolith core
Peak value is 10.73；BDHFE-7 is in the otolith section formation crest at 640-724 μm of otolith core, and highest peak is
62.60；BDHFE-8 is in the otolith section formation crest at 560-684 μm of otolith core, and highest peak is
143.64.8g/Kg gradient group strontium peak values are that normal mean value multiple is 2.14-2.22, and 64g/Kg gradient group strontiums peak value is normal equal
Value multiple rises to 6.60-18.27, shows the increase with concentration, and deposition of the strontium element on otolith is remarkably reinforced；Also tooth is illustrated
The continuous 10d of flounder juvenile fish feeds the mark feed of 8g/Kg and above chlorination content of strontium, and notable become occurs in deposition of the strontium on otolith
Change, i.e. the mark feed of 8g/Kg and above chlorination content of strontium can carry out strontium element Finger-print labelling method to Juvenile Japanese Flounder Paralichthys olivaceus otolith.
2nd, on different gradient group lefteye flounder otoliths strontium element EDS maps
Machine point is carried out to the EDS maps that 0,1,8, the 64 g/Kg gradient group strontium elements for recovering 30d after mark are deposited on otolith
Analysis, as a result as shown in Figure 2.0th, do not occur on 1g/Kg gradients group otolith " high strontium annulus ".BDHFE-5~BDHFE8 is occurred in that
Red " high strontium annulus ", it is obvious with the contrast of otolith background color.I.e. 8g/Kg and 64g/Kg gradients group can be entered to Juvenile Japanese Flounder Paralichthys olivaceus otolith
The obvious strontium element Finger-print labelling method of row.But 64g/Kg gradients group " high strontium annulus " is compared with the width of 8g/Kg gradient groups, and 64g/Kg gradient groups
Otolith edge color is extended down to outside " high strontium annulus " yellow compared with 8g/Kg gradients group, content of strontium is high compared with 8g/Kg gradients group.
3rd, conclusion：
External source strontium can carry out elemental fingerprints mark to lefteye flounder otolith.The water strontium chloride of various dose six refers to Juvenile Japanese Flounder Paralichthys olivaceus otolith element
Line labelling experiment, elemental fingerprints mark can be carried out to Juvenile Japanese Flounder Paralichthys olivaceus otolith by obtaining 8g/kg and the water strontium chloride of above dosage six.
Result of study shows that lefteye flounder fish is smaller to the sensitiveness of strontium, recovers 30d, 1g/Kg, 8g/Kg gradient group total length difference
Not significantly, 0g/Kg, 64g/Kg gradient group total length difference is not notable, 1g/Kg, 8g/Kg gradient group and 0g/Kg, 64g/Kg gradient group
Total length significant difference.（P<0.05）During mark, it is dead that each gradient group does not occur experiment fish.Strontium grows and dead to lefteye flounder fish
Have no adverse effect.
External source strontium can be to marking compared with the scale of small dimension lefteye flounder, and mark open-assembly time is short, with low cost, labeling method letter
Single, easy to operate, mark effect is steady and audible, can distinguish stocked population and natural population, fish-releasing mark is met very well
It is required that.
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above, the art it is general
Logical technical staff, the made variations, modifications, additions or substitutions in the essential scope of the present invention, falls within the guarantor of the present invention
Protect scope.
Claims (5)
1. a kind of lefteye flounder fish otolith rubidium marking method, it is characterised in that comprise the following steps：
（1）Six water strontium chlorides are well mixed with fish meal, make to contain strontium element in fish meal, obtain marking feed；
（2）Use mark feedstuff feeding lefteye flounder fish to be marked；
（3）Mark feed is fed daily 1-2 times, Feeding time is at least 3 days；
（4）Six water chlorination content of strontium at least account for the 0.8% of mark feed gross mass.
2. according to the lefteye flounder fish otolith rubidium marking method described in claim 1, it is characterised in that the step（4）In, it is described
Six water chlorination content of strontium account for the 1%-10% of mark feed gross mass.
3. according to the lefteye flounder fish otolith rubidium marking method described in claim 1 or 2, it is characterised in that the step（3）In,
Mark feed is fed daily 2 times, Feeding time is 4-10 days.
4. according to the lefteye flounder fish otolith rubidium marking method described in claim 1 or 2, it is characterised in that the step（1）In,
The fish meal is pellet, using biological phycocolloid as adhesive, and 1kg pellets, will at least using the biological phycocolloid of 10-15g
The water strontium chlorides of 8g six and the biological phycocolloid of 10-15g are dissolved in 100ml pure water, pour into watering can after being well mixed, are uniformly sprayed at 1kg
On grain feed, room temperature 24h dries.
5. according to the lefteye flounder fish otolith rubidium marking method described in claim 3, it is characterised in that the step（1）In, it is described
Fish meal is pellet, using biological phycocolloid as adhesive, and 1kg pellets, will at least 8g six using the biological phycocolloid of 10-15g
Water strontium chloride and the biological phycocolloid of 10-15g are dissolved in 100ml pure water, pour into watering can after being well mixed, are uniformly sprayed at the feeding of 1kg particles
On material, room temperature 24h dries.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552153A (en) * | 2021-07-27 | 2021-10-26 | 中国水产科学研究院淡水渔业研究中心 | Strontium finlet marking method for non-lethal freshwater fish and detection method thereof |
| CN115176743A (en) * | 2022-08-09 | 2022-10-14 | 浙江省海洋水产养殖研究所 | Clupanodon proliferation releasing marking method |
| CN115281140A (en) * | 2022-08-12 | 2022-11-04 | 复旦大学 | Aquatic product biological marker culture method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273418A (en) * | 2011-06-24 | 2011-12-14 | 中国水产科学研究院珠江水产研究所 | Fluorescent labeling method of fish otolith |
| CN104106481A (en) * | 2014-06-23 | 2014-10-22 | 中国水产科学研究院东海水产研究所 | Marking method for proliferation and releasing of large yellow croakers |
| WO2016077917A1 (en) * | 2014-11-19 | 2016-05-26 | Amirix Systems Inc. | Predation detection animal tracking tag |
| CN105638538A (en) * | 2016-03-30 | 2016-06-08 | 全国水产技术推广总站 | Bastard halibut ecological marking method for release counting |
| CN106086174A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院北戴河中心实验站 | A kind of accurate discriminating recapture Bastard halibut is released the method for fish |
-
2017
- 2017-05-24 CN CN201710373278.7A patent/CN107258647B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273418A (en) * | 2011-06-24 | 2011-12-14 | 中国水产科学研究院珠江水产研究所 | Fluorescent labeling method of fish otolith |
| CN104106481A (en) * | 2014-06-23 | 2014-10-22 | 中国水产科学研究院东海水产研究所 | Marking method for proliferation and releasing of large yellow croakers |
| WO2016077917A1 (en) * | 2014-11-19 | 2016-05-26 | Amirix Systems Inc. | Predation detection animal tracking tag |
| CN105638538A (en) * | 2016-03-30 | 2016-06-08 | 全国水产技术推广总站 | Bastard halibut ecological marking method for release counting |
| CN106086174A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院北戴河中心实验站 | A kind of accurate discriminating recapture Bastard halibut is released the method for fish |
Non-Patent Citations (1)
| Title |
|---|
| 张辉等: "大黄鱼耳石锶标志技术", 《中国水产科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552153A (en) * | 2021-07-27 | 2021-10-26 | 中国水产科学研究院淡水渔业研究中心 | Strontium finlet marking method for non-lethal freshwater fish and detection method thereof |
| CN115176743A (en) * | 2022-08-09 | 2022-10-14 | 浙江省海洋水产养殖研究所 | Clupanodon proliferation releasing marking method |
| CN115281140A (en) * | 2022-08-12 | 2022-11-04 | 复旦大学 | Aquatic product biological marker culture method |
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| CN107258647B (en) | 2019-11-08 |
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