CN113549586A - Fermentation medium of lactic acid bacteria - Google Patents
Fermentation medium of lactic acid bacteria Download PDFInfo
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- CN113549586A CN113549586A CN202111043118.9A CN202111043118A CN113549586A CN 113549586 A CN113549586 A CN 113549586A CN 202111043118 A CN202111043118 A CN 202111043118A CN 113549586 A CN113549586 A CN 113549586A
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- lactic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
A fermentation medium of lactic acid bacteria relates to the technical field of microorganisms. The formula is as follows: 30g/L glucose, 28g/L soybean meal, 14g/L corn flour and 28g/L, K whey powder2HPO43.0g/L, 2.5g/L of triammonium citrate, 5g/L of sodium acetate, and tween-801.25 mL/L, MgSO4·7H2O 0.2g/L、MnSO4·4H2O0.0625 g/L. The cost of the optimized culture medium is reduced by 4000 yuan/ton compared with the cost of the original MRS; the optimized culture conditions are as follows: temperature 37 deg.C, initial pH7.1, inoculum size 4%, standing for culturing lactobacillus under the above culture conditions for 36 hr to obtain fermentation broth with viable count of 6.07 × 109CFU/mL, significantly higher than that of the original MRS by 5.8X 108CFU/mL, can be used for fermentation production of lactic acid bacteria for fishing.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a fermentation culture medium of a strain of lactic acid bacteria with application value in fermentation production of fishery probiotics.
Background
The microecological preparation is an animal protection product for preventing and treating epidemic diseases of aquatic animals, is one of substitutes of antibiotics, and better meets the requirement of green ecological aquaculture. The lactobacillus is an important component of healthy flora in intestinal tracts of human beings and animals, does not produce exotoxin and exotoxin, is safe, nontoxic, harmless and free of side effects, can promote the intestinal tracts of the animals to absorb nutrient substances, inhibit pathogenic bacteria, regulate the balance of the intestinal flora, promote growth, improve immunity and purify water bodies, and is widely applied to aquaculture. However, the lactic acid bacteria also have the defects of high production cost, difficult preservation and the like, and the application of the lactic acid bacteria in aquaculture is restricted.
The invention obviously reduces the cost of lactobacillus fermentation production by low-value product substitution, fermentation culture medium optimization and the like. The lactobacillus fermentation culture medium determined by the invention not only can obviously reduce the cost of the culture medium, but also has good growth condition of the lactobacillus and high bacteria concentration of the fermentation liquid, thereby having unique advantages in the fermentation production of the lactobacillus for fishing.
Disclosure of Invention
The invention aims to provide a fermentation medium of a strain of lactic acid bacteria.
The formula of the fermentation medium of the lactic acid bacteria strain is as follows: 30g/L glucose, 28g/L soybean meal, 14g/L corn flour and 28g/L, K whey powder2HPO43.0g/L, 2.5g/L of triammonium citrate, 5g/L of sodium acetate, and tween-801.25 mL/L, MgSO4·7H2O0.2g/L、MnSO4·4H2O 0.0625g/L。
Glucose is used as a carbon source of the lactobacillus fermentation medium; soybean meal, corn flour and whey powder are used as nitrogen sources of the lactobacillus fermentation medium.
The fermentation medium of the lactic acid bacteria strain is applied to the production of the fishery probiotics, and the average concentration of the lactic acid bacteria in the culture solution of the optimized culture medium for 3 times of 250mL shake flask test is 5.265 multiplied by 109CFU/mL。
The invention has the following outstanding advantages:
the cost of the optimized culture medium is reduced by 4000 yuan/ton compared with the cost of the original MRS; the optimized culture conditions are as follows: temperature 37 deg.C, initial pH7.1, inoculum size 4% (v/v), static culture, culturing lactobacillus under the above culture conditions for 36 hr to obtain fermentation broth with viable count of 6.07 × 109CFU/mL, significantly higher than that of the original MRS by 5.8X 108CFU/mL. This shows that the lactic acid bacteria fermentation medium of the invention can be used for the fermentation production of lactic acid bacteria for fishing.
Drawings
FIG. 1 shows the effect of carbon source on the growth of lactic acid bacteria.
FIG. 2 is a graph showing the effect of nitrogen source on the growth of lactic acid bacteria.
FIG. 3 shows the interaction of glucose and nitrogen source on the growth of lactic acid bacteria.
Detailed Description
The following examples will further illustrate the present invention with reference to the accompanying drawings.
The formula of the fermentation medium of the lactic acid bacteria strain provided by the embodiment of the invention is as follows: 30g/L glucose, 28g/L soybean meal, 14g/L corn flour and 28g/L, K whey powder2HPO43.0g/L, 2.5g/L of triammonium citrate, 5g/L of sodium acetate, and tween-801.25 mL/L, MgSO4·7H2O 0.2g/L、MnSO4·4H2O 0.0625g/L。
According to the invention, through a single-factor test, glucose is found to be more beneficial to the growth of lactic acid bacteria than other carbon sources, and glucose is selected as the carbon source of the fermentation medium of lactic acid bacteria (see figure 1).
According to the invention, through single-factor tests, the soybean meal is found to be more beneficial to the growth of lactic acid bacteria than other nitrogen sources, and the soybean meal is selected as the nitrogen source of the lactic acid bacteria fermentation medium (see figure 2).
The fermentation culture medium of the lactic acid bacteria strain is applied to the production of the fishery probiotics, and after the lactic acid bacteria are cultured for 36 hours by using the culture medium, the number of viable bacteria in fermentation liquor is 6.07 multiplied by 109CFU/mL, significantly higher than that of the original MRS by 5.8X 108CFU/mL (see FIG. 3).
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (2)
1. The fermentation medium of a lactic acid bacteria strain is characterized by comprising the following components in percentage by weight: glucose 20g/L, whey powder 42g/L, K2HPO42g/L, 2g/L of ammonium citrate tribasic, 5g/L of sodium acetate and 801 mL/L, MgSO of Tween-4·7H2O 0.2g/L、MnSO4·4H2O 0.06g/L。
2. Use of a fermentation medium of a lactic acid bacterium according to claim 1 for the production of a probiotic for fish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111043118.9A CN113549586A (en) | 2021-09-07 | 2021-09-07 | Fermentation medium of lactic acid bacteria |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111043118.9A CN113549586A (en) | 2021-09-07 | 2021-09-07 | Fermentation medium of lactic acid bacteria |
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| CN113549586A true CN113549586A (en) | 2021-10-26 |
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| CN202111043118.9A Pending CN113549586A (en) | 2021-09-07 | 2021-09-07 | Fermentation medium of lactic acid bacteria |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597586A (en) * | 2009-07-12 | 2009-12-09 | 阜阳市大北农饲料有限责任公司 | A kind of product enzyme probiotic bacterium and production method thereof |
| CN105838654A (en) * | 2016-06-12 | 2016-08-10 | 江西省科学院微生物研究所 | Culture method of lactobacillus acidophilus high-density liquid |
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2021
- 2021-09-07 CN CN202111043118.9A patent/CN113549586A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597586A (en) * | 2009-07-12 | 2009-12-09 | 阜阳市大北农饲料有限责任公司 | A kind of product enzyme probiotic bacterium and production method thereof |
| CN105838654A (en) * | 2016-06-12 | 2016-08-10 | 江西省科学院微生物研究所 | Culture method of lactobacillus acidophilus high-density liquid |
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