CN113548318A - Storage facilities of umbilical cord mesenchymal stem cell exosome - Google Patents
Storage facilities of umbilical cord mesenchymal stem cell exosome Download PDFInfo
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- CN113548318A CN113548318A CN202110783451.7A CN202110783451A CN113548318A CN 113548318 A CN113548318 A CN 113548318A CN 202110783451 A CN202110783451 A CN 202110783451A CN 113548318 A CN113548318 A CN 113548318A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0273—Transport containers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/04—Partitions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/38—Devices for discharging contents
- B65D25/52—Devices for discharging successive articles or portions of contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/20—Containers, packaging elements or packages, specially adapted for particular articles or materials for incompressible or rigid rod-shaped or tubular articles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
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Abstract
The invention provides storage equipment for umbilical cord mesenchymal stem cell exosomes, and relates to the technical field of cell exosome storage. This storage facilities of umbilical cord mesenchymal stem cell exosome, including the shell body, the open-top of shell body, the inside of shell body is provided with the division board, forms a plurality of deposit room and a cold air room between the inside wall of division board and shell body, and the inboard of cold air room just is close to top fixedly connected with connection frame, and the top of connection frame can be dismantled and be connected with the upper cover plate, all is provided with the intercommunicating pore between cold air room and each deposit the room, and the intercommunicating pore inboard is provided with the baffler. When the storage rack in the storage chamber is taken out (namely one of the test tubes is taken out), the communicating hole is tightly blocked by the blocking plate under the action of the elastic piece, the storage chamber stops supplying cold air, other storage chambers do not receive influences, and the problem of storing other test tubes is not influenced when the test tubes are taken and placed can be realized.
Description
Technical Field
The invention relates to the technical field of cell exosome storage, in particular to storage equipment for umbilical cord mesenchymal stem cell exosomes.
Background
The umbilical cord mesenchymal stem cell is a multifunctional stem cell existing in umbilical cord tissues of newborns, can be differentiated into a plurality of tissue cells, and has wide clinical application prospect; the secreted exosome can perform intercellular transfer through protein and RNA components of the exosome, and plays the roles of tissue repair, immunosuppression, immunoregulation and the like similar to mesenchymal stem cells, and therefore, the exosome research is expected to become a key research direction in the future biomedical field.
The applicant finds that a Chinese patent discloses a storage device for mesenchymal stem cell exosomes through retrieval when applying the storage device, the application number of the storage device is '201910478304.1', the test tube to be taken can be taken out independently in the taking process, temperature change of other test tubes is avoided, the storage effect of the test tube is guaranteed, the test tube to be taken can be taken out only by adjusting the test tube to be taken to a specified position when the test tube is taken and placed, and operation is inconvenient.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides storage equipment for an umbilical cord mesenchymal stem cell exosome, and solves the problem that the steps of taking and placing test tubes by the existing storage equipment for the umbilical cord mesenchymal stem cell exosome are complex.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the storage equipment for the exosome of umbilical cord mesenchymal stem cells comprises an outer shell, wherein the top of the outer shell is open, a partition plate is arranged in the outer shell, a plurality of storage chambers and a cold air chamber are formed between the partition plate and the inner side wall of the outer shell, the inner side of the cold air chamber is close to a connecting frame fixedly connected with the top, the top of the connecting frame is detachably connected with an upper cover plate, communication holes are formed between the cold air chamber and each storage chamber, a baffle plate is arranged on the inner side of each communication hole, the side wall of the cold air chamber is fixedly connected with limiting frames on the left side and the right side of the baffle plate, an elastic cushion is arranged on the inner side of each limiting frame, a fixing plate is fixedly connected with the side wall of the cold air chamber above the limiting frames, a round hole is formed in the upper surface of the fixing plate, and a long screw rod is arranged in the round hole, the side surface of the long screw rod and the upper part of the fixed plate are in threaded connection with an adjusting nut, the bottom end of the long screw rod is fixedly connected with an elastic piece, the bottom end of the elastic piece is fixedly connected with the side surface of the blocking plate, the side surface of the blocking plate and the inner part of the storage chamber are fixedly connected with a transverse plate, and an air convection structure is arranged between the inner part of the cold air chamber and the outer part of the outer shell;
each the inside of deposit room is provided with the storage rack, the top fixedly connected with of storage rack goes up the roof, the top fixedly connected with handle of going up the roof, the back of storage rack is provided with the frame mouth, and when the storage rack was put into inside the deposit room completely, the diaphragm was located the inside of frame mouth, goes up the roof and is sealed with the top opening of deposit room.
Preferably, a sealing gasket is arranged at the contact part of the upper cover plate and the connecting frame, and the outer side surface of the connecting frame is fixedly connected with the inner side wall of the cold air chamber in a sealing manner.
Preferably, the inner bottom of the storage chamber is provided with a protective base plate, and the inner bottom of the storage chamber is provided with an overflow hole.
Preferably, the back fixedly connected with card foot of shell body, the equal fixedly connected with frame plate of the left and right sides face of shell body.
Preferably, the air convection structure includes air inlet filter mouth, air exhauster, the outside of air inlet filter mouth towards the shells, the air inlet and the air inlet filter mouth of air exhauster pass through the trachea and are connected, the gas outlet of air exhauster is located the inside of cold air room.
Preferably, a one-way air outlet is arranged between the inside of the cold air chamber and the outside of the outer shell.
Preferably, the side of the storage rack is provided with air holes.
Preferably, the inside of storage rack just is close to bottom fixedly connected with backup pad, the taper hole has been seted up to the upper surface of backup pad, the inside of storage rack just is located the top of backup pad and has the protection frame through sliding seat sliding connection, the side of protection frame is provided with C type mouth, the inside fixedly connected with rubber fixture block of C type mouth.
Preferably, the number of the C-shaped openings and the number of the taper holes are both provided with a plurality of the C-shaped openings, and the number of the C-shaped openings is equal to that of the taper holes.
The working principle is as follows: when the device is used, the device is placed in a refrigerating chamber or the air inlet filter opening 121 is communicated with cold air, the cold air is sucked into the cold air chamber 2 through the exhaust fan 122, then the cold air is independently supplied to each storage chamber 3 through the communication hole 5, when one storage rack 17 is taken out, the corresponding barrier plate 6 is reset under the action of the elastic piece 701, the communication hole 5 is blocked, and the cold supply in other storage chambers 3 cannot be influenced in the whole process;
elasticity of elastic component 11 can be along with using the number of times to increase and produce certain lax phenomenon, and the user is in the time of sealing performance between normal direction baffler 6 and intercommunicating pore 5 is not good, through rotating adjusting nut 10, can adjust long screw 9's position, with baffler 6 upwards sliding to the top, increases sealed effect, and it is worth noting that baffler 6 is when removing the top for: the transverse plate 25 is against the inner top of the communicating hole 5, and the elastic member 11 pulls the blocking plate 6 upward, in a balanced state.
(III) advantageous effects
The invention provides storage equipment for umbilical cord mesenchymal stem cell exosomes. The method has the following beneficial effects:
1. in the invention, the partition boards are arranged in the outer shell to form a plurality of storage chambers and cold air chambers, the cold air chambers can exchange cold air with the outside through an air convection structure, so that the inside has enough refrigeration effect, the communication holes are arranged between the cold air chambers and each storage chamber to independently supply cold air to the storage chambers, and the communicating hole is provided with a baffle plate structure, when the storage rack in the storage chamber is taken out (namely one test tube is taken out), the baffle plate can tightly block the communicating hole under the action of the elastic piece, the cold air supply to the storage chamber is stopped, the other storage chambers are not influenced, the problem that the storage of other test tubes is not influenced when the test tubes are taken and placed can be realized, the better using effect can be achieved, in addition, the test tubes in the storage chambers can be taken out individually, and a plurality of test tubes can be taken out simultaneously.
2. According to the invention, the storage rack is arranged, the supporting plate and the protective rack are arranged inside the storage rack, the test tube is clamped inside the C-shaped opening and then limited by the rubber clamping block, and the bottom of the test tube is positioned inside the taper hole, so that the test tube can be supported, and the test tube can be conveniently stored and taken out.
3. According to the invention, through the arrangement of the frame opening and the transverse plate structure, when the storage rack is completely placed in, the top of the frame opening presses the transverse plate downwards, so that the blocking plate moves downwards, the elastic part is stretched downwards, the communication hole is opened, cold air is exchanged, and when the storage rack is taken out, the storage rack automatically returns under the action of the elastic part.
Drawings
FIG. 1 is a schematic structural diagram of an outer casing according to the present invention;
FIG. 2 is a cross-sectional view taken along line A-A of FIG. 1;
FIG. 3 is a front view of the outer casing of the present invention;
FIG. 4 is a side view of the outer casing of the present invention;
FIG. 5 is a bottom view of the outer housing of the present invention;
FIG. 6 is a perspective view of the storage rack of the present invention;
fig. 7 is a schematic view of the back structure of the storage rack of the present invention.
Wherein, 1, an outer shell; 2. a cold air chamber; 201. a connecting frame; 202. an upper cover plate; 3. a storage chamber; 4. a partition plate; 5. a communicating hole; 6. a barrier plate; 7. a limiting frame; 701. an elastic pad; 8. a fixing plate; 9. a long screw; 10. adjusting the nut; 11. an elastic member; 12. an air convection arrangement; 121. an inlet air filter orifice; 122. an exhaust fan; 13. a one-way air outlet; 14. a frame plate; 15. clamping a pin; 16. an overflow hole; 17. a storage rack; 18. air holes are formed; 19. a frame opening; 20. an upper top plate; 21. a handle; 22. a support plate; 221. a taper hole; 23. a protective frame; 231. a C-shaped opening; 232. a rubber clamping block; 24. a protective backing plate; 25. a transverse plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
as shown in fig. 1-7, an embodiment of the present invention provides a storage device for umbilical cord mesenchymal stem cell exosomes, comprising an outer casing 1, a top opening of the outer casing 1, a partition plate 4 disposed inside the outer casing 1, a plurality of storage chambers 3 and a cold air chamber 2 formed between the partition plate 4 and an inner sidewall of the outer casing 1, a connection frame 201 fixedly connected to an inner side of the cold air chamber 2 near the top, an upper cover plate 202 detachably connected to a top of the connection frame 201, so that the cold air chamber 2 forms a sealed chamber, a communication hole 5 disposed between the cold air chamber 2 and each storage chamber 3 for supplying cold air individually, a barrier plate 6 disposed inside the communication hole 5, a limiting frame 7 fixedly connected to a sidewall of the cold air chamber 2 and located at left and right sides of the barrier plate 6, an elastic pad 701 disposed inside the limiting frame 7, the barrier plate 6 sliding inside the limiting frame 7, the blocking plate 6 is stable when sliding up and down, a fixing plate 8 is fixedly connected to the side wall of the cold air chamber 2 and located above the limiting frame 7, a round hole is formed in the upper surface of the fixing plate 8, a long screw 9 is arranged inside the round hole, an adjusting nut 10 is in threaded connection with the side face of the long screw 9 and located above the fixing plate 8, when the adjusting nut 10 rotates, the downward extending length of the long screw 9 can be adjusted, an elastic part 11 is fixedly connected to the bottom end of the long screw 9, the bottom end of the elastic part 11 is fixedly connected with the side face of the blocking plate 6, a transverse plate 25 is fixedly connected to the side face of the blocking plate 6 and located inside the storage chamber 3, and an air convection structure 12 is arranged between the inside of the cold air chamber 2 and the outside of the outer shell 1 and used for cooling the cold air chamber 2 from the outside;
each storage chamber 3's inside is provided with storage rack 17, storage rack 17's top fixedly connected with goes up roof 20, go up roof 20's top fixedly connected with handle 21, the person of facilitating the use upwards pulls out, storage rack 17's the back is provided with frame mouth 19, when storage rack 17 puts into storage chamber 3 inside completely, diaphragm 25 is located the inside of frame mouth 19, can realize pushing up diaphragm 25 downwards, it is sealed with the open-top of storage chamber 3 to go up roof 20, make storage chamber 3 and cold air chamber 2 communicate, simultaneously with storage chamber 3 with external sealed isolated.
The contact position of upper cover plate 202 and connection frame 201 is provided with seal ring, and the sealed fixed connection of the lateral surface of connection frame 201 and the inside wall of cold air chamber 2 increases the leakproofness of connection.
The inner bottom of the storage chamber 3 is provided with the protective base plate 24, so that the effect of safety protection is achieved, the bottom of the storage rack 17 is prevented from colliding with the inner bottom of the storage chamber 3 (theoretically, no collision point exists, but the upper top plate 20 deforms and collides during actual use), the overflow hole 16 is formed in the inner bottom of the storage chamber 3, liquefied water drops possibly exist during cold storage, the water drops slowly seep out from the overflow hole 16, the aperture ratio of the overflow hole 16 is small, and the refrigerating effect is basically not influenced.
The back fixedly connected with card foot 15 of shell body 1, the equal fixedly connected with frame plate 14 of the left and right sides face of shell body 1, the user of being convenient for wholly gets and puts shell body 1.
A one-way air outlet 13 is arranged between the inside of the cold air chamber 2 and the outside of the outer shell 1, and when the internal air pressure is too high, part of cold air is discharged.
The side of the storage rack 17 is provided with air holes 18.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The utility model provides a storage facilities of umbilical cord mesenchymal stem cell exosome, includes shell body (1), its characterized in that: the top opening of the outer shell (1), a plurality of storage chambers (3) and a cold air chamber (2) are formed between the inner side walls of the partition plates (4) and the outer shell (1), the inner side of the cold air chamber (2) is close to the top fixedly connected with connecting frame (201), the top of the connecting frame (201) is detachably connected with an upper cover plate (202), communicating holes (5) are formed between the cold air chamber (2) and each storage chamber (3), barrier plates (6) are arranged on the inner sides of the communicating holes (5), limit frames (7) are fixedly connected on the side walls of the cold air chamber (2) and located on the left side and the right side of the barrier plates (6), elastic cushions (701) are arranged on the inner sides of the limit frames (7), and fixing plates (8) are fixedly connected on the side walls of the cold air chamber (2) and located above the limit frames (7), the air conditioner is characterized in that a round hole is formed in the upper surface of the fixing plate (8), a long screw (9) is arranged inside the round hole, an adjusting nut (10) is connected to the side surface of the long screw (9) and the upper portion of the fixing plate (8) in a threaded mode, an elastic part (11) is fixedly connected to the bottom end of the long screw (9), the bottom end of the elastic part (11) is fixedly connected with the side surface of the blocking plate (6), a transverse plate (25) is fixedly connected to the side surface of the blocking plate (6) and the inner portion of the storage chamber (3), and an air convection structure (12) is arranged between the inner portion of the cold air chamber (2) and the outer portion of the outer shell (1);
each the inside of deposit room (3) is provided with storage rack (17), roof (20) on the top fixedly connected with of storage rack (17), the top fixedly connected with handle (21) of roof (20) on, the back of storage rack (17) is provided with frame mouth (19), and when storage rack (17) were put into inside of deposit room (3) completely, diaphragm (25) were located the inside of frame mouth (19), were gone up roof (20) and are sealed with the top opening of deposit room (3).
2. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: the contact part of the upper cover plate (202) and the connecting frame (201) is provided with a sealing gasket, and the outer side surface of the connecting frame (201) is fixedly connected with the inner side wall of the cold air chamber (2) in a sealing manner.
3. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: the inner bottom of the storage chamber (3) is provided with a protective backing plate (24), and the inner bottom of the storage chamber (3) is provided with an overflow hole (16).
4. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: the back fixedly connected with card foot (15) of shell body (1), the equal fixedly connected with frame plate (14) of the left and right sides face of shell body (1).
5. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: air convection structure (12) are including admitting air filtration mouth (121), air exhauster (122), admit air filtration mouth (121) is towards the outside of shell body (1), the air inlet and the filtration mouth of admitting air (121) of air exhauster (122) are connected through the trachea, the gas outlet of air exhauster (122) is located the inside of cold air room (2).
6. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: a one-way air outlet (13) is arranged between the inside of the cold air chamber (2) and the outside of the outer shell (1).
7. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: the lateral part of the storage rack (17) is provided with an air hole (18).
8. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 1, wherein: the inside of storage rack (17) just is close to bottom fixedly connected with backup pad (22), taper hole (221) have been seted up to the upper surface of backup pad (22), the inside of storage rack (17) just is located the top of backup pad (22) and has protection frame (23) through sliding seat sliding connection, the side of protection frame (23) is provided with C type mouth (231), the inside fixedly connected with rubber fixture block (232) of C type mouth (231).
9. The storage apparatus of umbilical cord mesenchymal stem cell exosomes according to claim 8, wherein: the number of the C-shaped openings (231) and the number of the taper holes (221) are multiple, and the number of the C-shaped openings (231) and the number of the taper holes (221) are equal.
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