CN113545881A - Nose dropping device for experimental animal - Google Patents
Nose dropping device for experimental animal Download PDFInfo
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- CN113545881A CN113545881A CN202111013044.4A CN202111013044A CN113545881A CN 113545881 A CN113545881 A CN 113545881A CN 202111013044 A CN202111013044 A CN 202111013044A CN 113545881 A CN113545881 A CN 113545881A
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- latex
- air inlet
- vesicle
- nasal
- injection tube
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- 238000010171 animal model Methods 0.000 title claims abstract description 37
- 239000004816 latex Substances 0.000 claims abstract description 46
- 229920000126 latex Polymers 0.000 claims abstract description 46
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 241001465754 Metazoa Species 0.000 claims abstract description 20
- 239000007924 injection Substances 0.000 claims description 40
- 238000002347 injection Methods 0.000 claims description 40
- 239000011324 bead Substances 0.000 claims description 9
- 241000699670 Mus sp. Species 0.000 claims description 7
- 230000000903 blocking effect Effects 0.000 claims description 5
- 241000700159 Rattus Species 0.000 claims 1
- 210000003928 nasal cavity Anatomy 0.000 abstract description 26
- 229940100662 nasal drops Drugs 0.000 abstract description 11
- 239000003814 drug Substances 0.000 abstract description 8
- 239000007923 nasal drop Substances 0.000 abstract description 7
- 238000007664 blowing Methods 0.000 abstract description 2
- 239000002184 metal Substances 0.000 abstract description 2
- 210000001331 nose Anatomy 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 11
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 210000004072 lung Anatomy 0.000 description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 210000003128 head Anatomy 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000006196 drop Substances 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011725 BALB/c mouse Methods 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000202934 Mycoplasma pneumoniae Species 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000004969 inflammatory cell Anatomy 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 206010001889 Alveolitis Diseases 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010003598 Atelectasis Diseases 0.000 description 1
- 206010006448 Bronchiolitis Diseases 0.000 description 1
- 208000029523 Interstitial Lung disease Diseases 0.000 description 1
- 208000001572 Mycoplasma Pneumonia Diseases 0.000 description 1
- 208000007123 Pulmonary Atelectasis Diseases 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 201000001155 extrinsic allergic alveolitis Diseases 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 208000022098 hypersensitivity pneumonitis Diseases 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Abstract
The invention discloses a nasal dropper for experimental animals, relates to the field of veterinarians, and aims to solve the problem that the nasal dropper for the experimental animals at present causes liquid medicine to overflow and injure the nasal cavity of the animal. The invention adopts the latex vesicles as the animal nasal cavity expanding part, the latex vesicles of the soft part are blown to be expanded in an air blowing mode and then support the nasal cavity, so that the nasal drops smoothly enter the animal nasal cavity, the latex vesicles are tightly attached to the inner wall of the nasal cavity in the circumferential direction, the nasal drops can be prevented from overflowing, the dosage loss is avoided, and compared with parts made of metal and the like, the nasal cavity cannot be damaged. The invention is applied to the field of experimental animals.
Description
Technical Field
The invention relates to the field of veterinarians, in particular to a nasal dropper for experimental animals.
Background
In the animal experiment process, the administration route has a lot of, and the nasal drip is a general mode of administration, does not have the apparatus of special nasal drip administration at present, and current nasal drip ware leads to the dropping liquid to spill easily, or struts the problem that the nasal cavity part leads to animal nasal cavity to harm easily.
Disclosure of Invention
The invention aims to solve the problems that liquid medicine overflows and the nasal cavity of an animal is damaged due to the existing nasal dropper for experimental animals, and provides the nasal dropper for the experimental animals.
The experimental animal nose drop device comprises an injection tube and a latex vesicle;
the injection tube consists of an injection tube body, an air inlet push rod and a liquid inlet push rod; an air inlet groove is formed in the periphery of the injection tube body along the circumferential direction of the injection tube body, two ends of the air inlet groove are opened, and one end of the air inlet push rod is arranged in the air inlet groove; one end of the liquid inlet push rod is arranged in the cavity of the injection tube, and the push handle of the liquid inlet push rod drives the push handle of the air inlet push rod to push together when pushing;
the front end of the latex vesicle is sleeved on an air inlet groove at the head of the injection tube body, and an air inlet is communicated with an air outlet of the air inlet groove;
the front end of the injection tube is provided with a needle tube, a liquid inlet of the needle tube is communicated with a liquid outlet of the tube body of the injection tube, the liquid outlet penetrates through the latex vesicle and is positioned outside the latex vesicle, and a gas outlet is formed in the latex vesicle close to the liquid outlet of the injection tube.
Furthermore, the experimental animal nose dropper is also provided with a limit screw and a scale indicating rod; scales are carved on the outer tube wall of the injection tube body, a scale indicating rod is arranged at the front end of a handle of the air inlet push rod, which is connected with a limit screw rod, and the front end of the scale indicating rod indicates the scales on the outer tube wall; the scale indication rod is matched with the outer pipe wall of the injection pipe body.
Furthermore, a plurality of air outlet plugging beads are also arranged in the latex bubble.
Furthermore, the front end and the rear end of the latex vesicle are provided with openings, the head of the needle tube penetrates through the opening at the front end of the latex vesicle, the outer wall of the needle tube is adhered to the latex vesicle, the opening at the rear end of the latex vesicle is sleeved on the outer wall of the air outlet of the air inlet groove and adhered to the air outlet, and a plurality of air outlet plugging beads are placed in the latex vesicle.
Furthermore, the diameter of the air outlet blocking bead is larger than the diameter of the air outlet and the diameter of the air outlet of the air inlet groove.
Furthermore, an opening is formed in the middle of a pushing handle of the air inlet push rod, and the tail end of the liquid inlet push rod penetrates through the opening of the pushing handle of the air inlet push rod and is arranged in the injection tube body.
Further, the experimental animal is a mouse.
The invention has the following beneficial effects:
the invention adopts the latex vesicles as the animal nasal cavity expanding part, the latex vesicles of the soft part are blown to be expanded in an air blowing mode and then support the nasal cavity, so that the nasal drops smoothly enter the animal nasal cavity, the latex vesicles are tightly attached to the inner wall of the nasal cavity in the circumferential direction, the nasal drops can be prevented from overflowing, the dosage loss is avoided, and compared with parts made of metal and the like, the nasal cavity cannot be damaged. More importantly, the front end of the latex vesicle is provided with the air outlet, the direction of air outlet of the air outlet is the same as the direction of nasal drops entering the nasal cavity, the latex vesicle can not be rapidly shrunk while air is blown out by controlling the opening amount, and the latex vesicle is ensured to be tightly attached to the inner wall of the nasal cavity in the circumferential direction all the time when the nasal drops are injected. Moreover, the blown gas can push the nasal drops into the nasal cavity, and the outflow is reduced. The invention sets air inlet groove around the injection tube, sets air inlet push rod in it, drives the air inlet push rod to push in it by the push of the liquid inlet push rod, the air enters the latex vesicle, the latex vesicle swells rapidly, the nose drops enter the nasal cavity, because the air inlet groove and the cavity length of the injection tube are similar, the liquid inlet push rod and the air inlet push rod almost reach the bottom at the same time, therefore, the air entering the latex vesicle is almost finished after the nose drops almost enter the nasal cavity, the latex vesicle is also reduced gradually, thereby the smooth dropping of the nose drops is finished, and the overflow of the nose drops is prevented.
The scheme of the invention can effectively solve the problems that the nasal drip of the experimental animal causes the liquid medicine to overflow and the nasal cavity of the animal is damaged, and has simple structure and strong practicability.
Drawings
FIG. 1 is a schematic structural view of a nasal dropper for experimental animals according to the present invention;
FIG. 2 is a photograph showing the experimental animal of example 1 before and after dropping the nose of a mouse; wherein, the left picture of each group of pictures is before the nose drop, and the right picture is after the nose drop;
FIG. 3 is a HE staining pattern of a blank group and an infected group of mice after dropping the nose of the experimental animal to the nose of the experimental animal of example 1.
Detailed Description
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples for carrying out the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in practice.
To make the objects, aspects and advantages of the embodiments of the present invention more apparent, the following detailed description clearly illustrates the spirit of the disclosure, and any person skilled in the art, after understanding the embodiments of the disclosure, may make changes and modifications to the technology taught by the disclosure without departing from the spirit and scope of the disclosure.
The exemplary embodiments of the present invention and the description thereof are provided to explain the present invention and not to limit the present invention.
Example 1
The experimental animal nose dropper of the embodiment is characterized by comprising an injection tube 1 and a latex vesicle 2;
the injection tube 1 consists of an injection tube body 11, an air inlet push rod 12 and a liquid inlet push rod 13; an air inlet groove 5 is formed in the periphery of the injection tube body 11 along the circumferential direction of the injection tube body, two ends of the air inlet groove 5 are opened, and one end of an air inlet push rod 12 is arranged in the air inlet groove 5; one end of the liquid inlet push rod 13 is arranged in the cavity of the injection tube 1, and the push handle of the liquid inlet push rod 13 drives the push handle of the air inlet push rod 12 to push together when pushing; the front end of the latex vesicle 2 is sleeved on an air inlet groove 5 at the head of the injection tube body 11, and an air inlet is communicated with an air outlet of the air inlet groove 5;
the front end of the injection tube 1 is provided with a needle tube 14, the liquid inlet of the needle tube 14 is communicated with the liquid outlet of the injection tube body 11, the liquid outlet penetrates through the latex vesicle 2 and is positioned outside the latex vesicle 2, and the latex vesicle 2 close to the liquid outlet of the injection tube 1 is provided with a gas outlet 21.
The experimental animal nose dropper is also provided with a limit screw 3 and a scale indicating rod 4; scales are carved on the outer tube wall of the injection tube body 11, a scale indicating rod 4 is arranged at the front end of a handle of the air inlet push rod 12, which is connected with the limit screw 3, and the front end of the scale indicating rod 4 indicates the scales on the outer tube wall; the scale indication rod 4 is matched with the outer pipe wall of the injection pipe body 11. And a plurality of air outlet plugging beads are also arranged in the latex vesicle 2.
The front end and the rear end of the latex vesicle 2 are provided with openings, the head of the needle tube 14 penetrates through the opening at the front end of the latex vesicle 2, the outer wall of the needle tube is adhered with the latex vesicle 2, the opening at the rear end of the latex vesicle 2 is sleeved on the outer wall of the air outlet of the air inlet groove 5 and is adhered with the air outlet, and a plurality of air outlet plugging beads are arranged in the latex vesicle 2.
The diameter of the air outlet blocking bead is larger than the diameter of the air outlet 21 and the diameter of the air outlet of the air inlet groove 5.
The middle of the push handle of the air inlet push rod 12 is provided with an opening, and the tail end of the liquid inlet push rod 13 penetrates through the opening of the push handle of the air inlet push rod 12 and is arranged in the injection tube body 11.
After the nasal dropper of the embodiment is adopted to carry out nasal dropping on a mouse, the nasal dropping effect is observed, and the result shows that the overflow of the nasal dropping liquid is not found and no nasal cavity injury occurs.
1 materials and methods
1.1 Main test Instrument
Example 1 nasal drip for laboratory animal
1.2 animals
20 BALB/c mice have the physical mass of 18-22g, and are provided by the department of laboratory animals of Harbin medical university, with the license number SCXK (black) 2019-.
1.3 Primary reagents
Mycoplasma pneumoniae standards (ATCC15531) were purchased from American TypeCultureCo, cultured in fetal bovine serum for 24h, grown in PPLO medium containing 20% fetal bovine serum and 10% yeast, and passaged every 7 d. PPLO medium (Becton, Dickinson and Company, USA); yeast (Angel Yeast, Inc.); fetal bovine serum (Gibco, USA); hematoxylin, eosin stain (Nanjing Innovation Co.); physiological saline.
1.4 Main instrumentation
KD-TS3A auto-tissue dehydrator (Zhejiang Jinhua Kedi instruments, Inc.); KD-BM biological tissue embedding machine (Zhejiang Jinhuakedi instruments, Ltd.); RM2235 tissue microtome (lycra, germany); BX4-1 biomicroscope (OLYMPUS Co.); MDF-382E-80 deg.C low temperature refrigerator (SANYO, Japan); model ST-16R low temperature high speed centrifuge (ThermoFisher Scientific, USA; model LXJ-n ordinary low speed centrifuge (Shanghai medical Analyzer) 20uL, 200uL, 1000uL micro-applicator (Eppendoff), BP211D analytical balance (Sartorius).
1.5 methods
1.5.1 animal models were established mainly with nasal droppers, 20-22 g BALB/c mice, randomly divided into 2 groups, 10 infected groups (Mycoplasma pneumoniae infection) and 10 blank groups. And (3) carrying out ether anesthesia on the infected mice, sucking 20uL10CCU/mL mycoplasma pneumoniae body fluid by using an experimental animal nasal dropper, grabbing the mice, injecting the liquid into the nasal cavity by using the mouse nasal dropper, and continuously sucking for 3 d. The blank group was treated with a nasal dropper of laboratory animals and the same volume of MP liquid medium was aspirated. After completion, mice were sacrificed by cutting the marrow, lung tissue was isolated and left lung tissue was fixed in 10% formaldehyde for HE staining. The experiment was repeated 3 times.
1.5.2HE staining, the most common method for detecting changes in tissue structure. Fixing lung tissue in 10% formaldehyde for more than 48 hr, dehydrating, clearing, embedding, slicing, dewaxing, hydrating, staining with hematoxylin and eosin, dehydrating, and sealing with neutral gum. Lung tissue sections HE stained nuclei with blue cytoplasm, connective tissue, muscle, etc., all stained in different degrees of red color and changes in lung tissue pathology were observed under an optical microscope. Pathological scores ranged from 0 (no change) to 16 (maximal change), with scores ranging from mild to moderate 1-4 for bronchiolitis, perivasculitis, interstitial pneumonia and alveolitis.
2 results
2.1 no liquid medicine flows out in the nasal drip process, ensuring that the medicine is completely injected into the nasal cavity; the nasal cavity of the mouse is observed after the nasal drip, and the nasal cavity of the mouse is not damaged except the stimulation of the medicine. See fig. 2.
2.2 pathological examination of Lung tissue
HE staining shows that the lung tissue morphology of the blank mice has no abnormal change, clear structure, complete alveolar space, regular arrangement and no inflammatory cell infiltration. The lung tissue epithelial cells of the infected mice fall off, excessive red blood cells in the bronchia interstitial thickened alveolar wall capillaries and a large amount of inflammatory cells at intervals infiltrate into the alveolar wall, and the alveolar wall collapses, so that focal atelectasis can occur. See fig. 3.
Discussion of 3
The experimental result shows that the nasal drip model is made for the mouse by using the nasal drip device of the experimental animal, the HE dyeing proves that the model making is successful, the medicine enters the lung of the mouse to infect the mouse, the nasal cavity is not damaged, and the influence of other objective factors on the model making during the model making period is greatly reduced. The experimental animal nose dropper plays a great role in the experimental animal nose dropper modeling process, the medicine can accurately reach the model without error, the damage to the experimental animal is minimized, and the external influence factors of the objective environment of the experimental animal are not influenced.
Claims (7)
1. The experimental animal nose dropper is characterized by comprising an injection tube (1) and a latex vesicle (2);
the injection tube (1) consists of an injection tube body (11), an air inlet push rod (12) and a liquid inlet push rod (13); an air inlet groove (5) is formed in the periphery of the injection tube body (11) along the circumferential direction of the injection tube body, two ends of the air inlet groove (5) are opened, and one end of an air inlet push rod (12) is arranged in the air inlet groove (5); one end of the liquid inlet push rod (13) is arranged in the cavity of the injection tube (1), and the push handle of the liquid inlet push rod (13) drives the push handle of the air inlet push rod (12) to push together when pushing;
the front end of the latex vesicle (2) is sleeved on an air inlet groove (5) at the head of the injection tube body (11), and an air inlet is communicated with an air outlet of the air inlet groove (5);
the front end of the injection tube (1) is provided with a needle tube (14), a liquid inlet of the needle tube (14) is communicated with a liquid outlet of the injection tube body (11), the liquid outlet penetrates through the latex vesicle (2) and is positioned outside the latex vesicle (2), and a gas outlet (21) is formed in the latex vesicle (2) close to the liquid outlet of the injection tube (1).
2. The nasal dropper for laboratory animals according to claim 1, wherein the nasal dropper for laboratory animals is further provided with a limit screw (3) and a scale indication rod (4); scales are marked on the outer tube wall of the injection tube body (11), a scale indicating rod (4) is arranged at the front end of a handle of the air inlet push rod (12) connected with the limiting screw (3), and the front end of the scale indicating rod (4) indicates the scales on the outer tube wall; the scale indication rod (4) is matched with the outer pipe wall of the injection pipe body (11).
3. The nasal dropper for laboratory animals according to claim 1, wherein the latex vesicle (2) further comprises a plurality of outlet blocking beads.
4. The nasal dropper for experimental animals according to claim 1 or 3, wherein the front end and the rear end of the latex vesicle (2) are open, the head of the needle tube (14) passes through the front end opening of the latex vesicle (2), the outer wall of the needle tube is adhered to the latex vesicle (2), the rear end opening of the latex vesicle (2) is sleeved on the outer wall of the air outlet of the air inlet groove (5) and adhered to the air outlet, and a plurality of air outlet blocking beads are placed in the latex vesicle (2).
5. The nasal dropper for laboratory animals according to claim 4, wherein the diameter of the outlet blocking bead is greater than the diameter of the outlet (21) and the diameter of the outlet of the inlet channel (5).
6. The nasal dropper for laboratory animals according to claim 1, wherein the air inlet rod (12) has an opening at the middle of its handle, and the end of the liquid inlet rod (13) passes through the opening of the handle of the air inlet rod (12) and is disposed in the syringe tube (11).
7. The nasal dropper for laboratory animals according to claim 1, wherein the laboratory animals are rats and mice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111013044.4A CN113545881A (en) | 2021-08-31 | 2021-08-31 | Nose dropping device for experimental animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111013044.4A CN113545881A (en) | 2021-08-31 | 2021-08-31 | Nose dropping device for experimental animal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113545881A true CN113545881A (en) | 2021-10-26 |
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ID=78106265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111013044.4A Pending CN113545881A (en) | 2021-08-31 | 2021-08-31 | Nose dropping device for experimental animal |
Country Status (1)
| Country | Link |
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| CN (1) | CN113545881A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1160632A (en) * | 1967-04-05 | 1969-08-06 | Volynskoe Oblastnoe Upravlenie | Improvements in or relating to Nasogastric Appliances |
| CN209122533U (en) * | 2018-11-07 | 2019-07-19 | 首都医科大学宣武医院 | A kind of device for experiment mice intranasal drop medicine |
| US20210060233A1 (en) * | 2018-05-11 | 2021-03-04 | Fluchem Ltd | Device and method for injecting liquid drug into nasal cavity and paranasal sinuses |
-
2021
- 2021-08-31 CN CN202111013044.4A patent/CN113545881A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1160632A (en) * | 1967-04-05 | 1969-08-06 | Volynskoe Oblastnoe Upravlenie | Improvements in or relating to Nasogastric Appliances |
| US20210060233A1 (en) * | 2018-05-11 | 2021-03-04 | Fluchem Ltd | Device and method for injecting liquid drug into nasal cavity and paranasal sinuses |
| CN209122533U (en) * | 2018-11-07 | 2019-07-19 | 首都医科大学宣武医院 | A kind of device for experiment mice intranasal drop medicine |
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