CN113545341A - Cold-temperature disinfectant and preparation method and application method thereof - Google Patents
Cold-temperature disinfectant and preparation method and application method thereof Download PDFInfo
- Publication number
- CN113545341A CN113545341A CN202110845550.3A CN202110845550A CN113545341A CN 113545341 A CN113545341 A CN 113545341A CN 202110845550 A CN202110845550 A CN 202110845550A CN 113545341 A CN113545341 A CN 113545341A
- Authority
- CN
- China
- Prior art keywords
- disinfectant
- cold
- disinfection
- temperature
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000645 desinfectant Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims description 17
- 238000002360 preparation method Methods 0.000 title claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 77
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 24
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 24
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000003756 stirring Methods 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 8
- 238000007865 diluting Methods 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 230000003137 locomotive effect Effects 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 4
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 abstract description 2
- 239000004155 Chlorine dioxide Substances 0.000 abstract description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 abstract description 2
- 239000001569 carbon dioxide Substances 0.000 abstract description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 2
- 231100000357 carcinogen Toxicity 0.000 abstract description 2
- 239000003183 carcinogenic agent Substances 0.000 abstract description 2
- 239000000460 chlorine Substances 0.000 abstract description 2
- 229910052801 chlorine Inorganic materials 0.000 abstract description 2
- 235000019398 chlorine dioxide Nutrition 0.000 abstract description 2
- 229960001701 chloroform Drugs 0.000 abstract description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract description 2
- 238000003958 fumigation Methods 0.000 abstract description 2
- 230000007794 irritation Effects 0.000 abstract description 2
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 abstract description 2
- 230000005180 public health Effects 0.000 abstract description 2
- 230000007281 self degradation Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 36
- 238000012360 testing method Methods 0.000 description 29
- 230000036541 health Effects 0.000 description 8
- 230000007797 corrosion Effects 0.000 description 7
- 238000005260 corrosion Methods 0.000 description 7
- 239000002655 kraft paper Substances 0.000 description 7
- 229910000975 Carbon steel Inorganic materials 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 4
- 229910052782 aluminium Inorganic materials 0.000 description 4
- 239000010962 carbon steel Substances 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 239000010935 stainless steel Substances 0.000 description 4
- 229910001220 stainless steel Inorganic materials 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 241000991587 Enterovirus C Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241000274177 Juniperus sabina Species 0.000 description 1
- 208000000474 Poliomyelitis Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 210000003501 vero cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Abstract
The invention discloses a cold-temperature disinfectant, which is characterized by comprising a disinfectant, a disinfection auxiliary agent and water; the disinfectant comprises: 4-6% of peroxyacetic acid, 20-30% of hydrogen peroxide and the balance of water; the disinfection auxiliary agent comprises 30% of glycerol and 70% of 1-3 propylene glycol. The product of the invention has excellent stability and the strongest effect of killing the living microorganisms. Can replace the prior fumigation mode using aldehyde disinfectant and chlorine dioxide disinfectant, so as to reduce the irritation to human and animals to the minimum and not generate carcinogens such as trichloromethane and the like. The product of the invention has the safety of self-degradation into carbon dioxide and water while acting, the application range is popularized, and the damage to the environment caused by excessive use of a chlorine-containing disinfectant and excessive concentration use of peracetic acid and the like is avoided when a major public health incident is in an emergency.
Description
Technical Field
The invention relates to the technical field of sanitation and disinfection, in particular to a cold-temperature disinfection solution and a preparation method and an application method thereof.
Background
Peroxyacetic acid and hydrogen peroxide have been used as disinfectants for almost 60-70 years. Both of them are strong oxidizers, and have the characteristic of easy explosion when the concentration is high, while the peroxyacetic acid is also very unstable and has short storage life. These characteristic properties severely limit their widespread use. The peroxygen disinfectant is a novel disinfectant developed along with the development of the additive industry in the seventies of the last century. The development of various active medium auxiliaries has made it possible to achieve systems which previously could not be stably co-present. Over a decade, with the continuous development of research, the efficacy of killing microorganisms is continuously improved, the stabilization period is also continuously prolonged, and the product becomes a high-efficiency mature practical product.
Therefore, the technical problem to be solved by those skilled in the art is to research and develop a disinfectant with stronger stability than the existing peroxide disinfectant so as to have more excellent disinfecting effect.
Disclosure of Invention
In view of the above, the present invention provides a cold-warm disinfection solution, a preparation method and an application method thereof, in order to achieve the above purpose, the present invention adopts the following technical scheme:
a cold-temperature disinfection solution comprises a disinfection solution, a disinfection auxiliary agent and water;
the disinfectant comprises the following raw materials in percentage by mass: 4-6% of peroxyacetic acid, 20-30% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following raw materials in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol;
further, the mass ratio of the disinfectant, the disinfection auxiliary agent and the water is 1:0.3-1: 50-100;
the invention also provides a preparation method of the cold-temperature disinfection solution, which comprises the following steps:
(1) weighing the raw materials according to the dosage;
(2) diluting the disinfectant with water at low temperature;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and uniformly stirring to obtain the cold-warm disinfection solution.
Further, the low temperature condition in the step (2) is-20 ℃ to 10 ℃.
The beneficial effect of adopting the further scheme is that: can not freeze for 8 hours at the temperature of minus 20 ℃ and minus 10 ℃ respectively, and the disinfection time is finished in 20 minutes.
Further, in the step (3), the stirring speed is 100r/min, and the stirring time is 10-15 min.
The invention also provides an application method of the cold-warm disinfectant, wherein the cold-warm disinfectant is atomized and sprayed by a spraying locomotive, and the amount of the sprayed liquid medicine is 35-40 ml per square meter.
The invention has the beneficial effects that:
(1) compared with domestic peracetic acid disinfectants, the disinfectant has excellent stability and the strongest effect of killing living microorganisms.
(2) Can replace the prior fumigation mode using aldehyde disinfectant and chlorine dioxide disinfectant, so as to reduce the irritation to human and animals to the minimum and not generate carcinogens such as trichloromethane and the like.
(3) The product of the invention has the safety of self-degradation into carbon dioxide and water while acting, the application range is popularized, and the damage to the environment caused by excessive use of a chlorine-containing disinfectant and excessive concentration use of peracetic acid and the like is avoided when a major public health incident is in an emergency.
(4) Compared with the guide index published by the state, the concentration of peracetic acid in the product of the invention needs to be reduced to 0.05 percent and 0.1 percent by 0.2-0.4 percent, thereby greatly reducing the use of excessive disinfectants and being beneficial to environmental protection and personnel safety.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Cold-temperature disinfection solution:
(1) weighing 1kg of disinfectant, 1kg of disinfection auxiliary agent and 100kg of water;
the disinfectant comprises the following components in percentage by mass: 6% of peroxyacetic acid, 20% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following components in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol;
(2) diluting the disinfectant with water at-20 deg.C;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and stirring for 15min at a speed of 100r/min to obtain the cold-temperature disinfection solution.
Example 2
Cold-temperature disinfection solution:
(1) weighing 1kg of disinfectant, 0.3kg of disinfection auxiliary agent and 50kg of water;
the disinfectant comprises the following components in percentage by mass: 4% of peroxyacetic acid, 30% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following components in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol;
(2) diluting the disinfectant with water at-10 deg.C;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and stirring for 10min at a speed of 100r/min to obtain the cold-temperature disinfection solution.
Example 3
Cold-temperature disinfection solution:
(1) weighing 1kg of disinfectant, 0.5kg of disinfection auxiliary agent and 70kg of water;
the disinfectant comprises the following components in percentage by mass: 5% of peroxyacetic acid, 25% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following components in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol;
(2) diluting the disinfectant with water at-10 deg.C;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and stirring for 12min at a speed of 100r/min to obtain the cold-temperature disinfection solution.
Example 4
Cold-temperature disinfection solution:
cold-temperature disinfection solution:
(1) weighing 1kg of disinfectant, 0.8kg of disinfection auxiliary agent and 60kg of water;
the disinfectant comprises the following components in percentage by mass: 4.5% of peroxyacetic acid, 24.5% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following components in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol;
(2) diluting the disinfectant with water at-20 deg.C;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and stirring for 14min at a speed of 100r/min to obtain the cold-temperature disinfection solution.
Example 5
Cold-temperature disinfection solution:
cold-temperature disinfection solution:
(1) weighing 1kg of disinfectant, 0.4kg of disinfection auxiliary agent and 90kg of water;
the disinfectant comprises the following components in percentage by mass: 5.5% of peroxyacetic acid, 27.5% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following components in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol;
(2) diluting the disinfectant with water at-15 deg.C;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and stirring for 13min at a speed of 100r/min to obtain the cold-temperature disinfection solution.
Test example 1
1.1
The cold-temperature disinfection solution prepared in the example 5 is subjected to a corrosivity test under the constant temperature condition of-10 ℃, and the test result shows that the corrosion rates of the cold-temperature disinfection solution on carbon steel, copper, aluminum and stainless steel are 0.2225mm/a, 0.3329mm/a, 0.0606mm/a and 0.0031mm/a respectively; moderate corrosion for carbon steel and copper, slight corrosion for aluminum, and basically no corrosion for stainless steel.
1.2
The cold-temperature disinfection solution prepared in the example 5 is subjected to a corrosivity test under the constant temperature condition of-20 ℃, and the test results show that the corrosion rates of carbon steel, copper, aluminum and stainless steel are respectively 0.0450mm/a, 0.0967mm/a, 0.0394mm/a and 0 mm/a; the corrosion resistance is slight to carbon steel, copper and aluminum, and basically no corrosion to stainless steel.
Test example 2
An apparatus
1. Test strains: poliovirus type I (PV-I/sabin/2003) vaccine strain, provided by the national polio laboratory.
2. Cell lines: VERO cells, passage 5 to 10.
3. Test samples: the cold temperature sterilizing solution prepared in example 5.
4. Test carrier: sterilized kraft paper (5 cm. times.5 cm) was fixed to 6 sides of the carton.
Second, method
1. Preparation of spinal gray virus vector: according to the technical Specification for disinfection 2.1.1.10.7, 2.1.2.9 of Ministry of health, sterilized kraft paper (5cm multiplied by 5cm) is adopted as a carrier, 100 mu L of virus suspension is dripped on the carrier, the logarithm value of the average virus titer (TCID50) is determined to be between 5 and 7, and the carrier is dried and placed for 30min at the constant temperature of minus 20 ℃ for later use.
2. Simulation field test of object surface disinfection: the experiments were carried out according to 2.1.2.9 in the Disinfection Specification of the Ministry of health (202 th edition). The cold chain goods carton with the temperature of minus 20 ℃ is placed in a freezer with the temperature of minus 20 ℃ for more than 24 hours and taken out, and the contamination carrier is placed in the freezer with the temperature of minus 20 ℃ after being fixed on 6 surfaces of the carton. Spraying and disinfecting the test solution of the disinfectant stock solution in a freezer by using a handheld spray can, taking down the carrier on the carton by aseptic operation after 20min of action, and placing the carrier in a 15mL centrifuge tube containing a neutralizer, wherein the test group is formed; meanwhile, the same batch of contaminated carriers is used as a control group, 6 temperature probes of a temperature tester are placed on 6 surfaces of the carton, and temperature measurement is respectively carried out. The test was repeated 5 times at-20 ℃.
And thirdly, the results are shown in 5 repeated tests that the cold-warm disinfection solution prepared in the embodiment 5 of the invention has spraying effect for 20min at the temperature of-20 ℃, the average inactivation log value of poliovirus infected on 6 surface kraft papers of a carton is 3.78, and the killing log value of each sample is more than 3.00 (the results are shown in table 1).
TABLE 1
| Serial number | Surface temperature range (. degree. C.) of the paper box | Log value of inactivation |
| 1-1 | -20.3～-20.0 | 4.17 |
| 1-2 | -20.4～-20.0 | 3.83 |
| 1-3 | -20.7～-20.2 | 4.17 |
| 1-4 | -20.3～-20.2 | 4.17 |
| 1-5 | -20.2～-20.0 | 4.00 |
| 1-6 | -20.3～-20.0 | 3.83 |
| 2-1 | -20.7～-20.3 | 3.83 |
| 2-2 | -20.4～-20.2 | 3.83 |
| 2-3 | -20.3～-20.0 | 4.00 |
| 2-4 | -20.8～-20.4 | 4.00 |
| 2-5 | -20.3～-20.2 | 3.83 |
| 2-6 | -20.6～-20.2 | 4.83 |
| 3-1 | -20.6～-20.2 | 4.34 |
| 3-2 | -20.2～-20.0 | 4.17 |
| 3-3 | -20.3～-20.0 | 4.00 |
| 3-4 | -20.8～-20.2 | 3.34 |
| 3-5 | -20.7～-20.3 | 3.17 |
| 3-6 | -20.9～-20.8 | 3.00 |
| 4-1 | -20.6～-20.4 | 3.34 |
| 4-2 | -20.4～-20.1 | 3.17 |
| 4-3 | -20.3～-20.0 | 4.17 |
| 4-4 | -20.7～-20.2 | 4.00 |
| 4-5 | -20.3～-20.2 | 4.00 |
| 4-6 | -20.2～-20.0 | 3.34 |
| 5-1 | -20.7～-20.3 | 3.83 |
| 5-2 | -20.4～-20.2 | 3.17 |
| 5-3 | -20.2～-20.1 | 3.00 |
| 5-4 | -20.8～-20.3 | 3.83 |
| 5-5 | -20.4～-20.1 | 3.17 |
| 5-6 | -20.8～-20.2 | 3.83 |
| Mean value of | / | 3.78 |
Example (c): tables 1-1 to 1-6 in table 1 show the 6 surfaces of the carton, respectively.
Test example 3
According to the method of the experimental example 2, under the constant temperature condition of-10 ℃, the cold-temperature disinfection solution of the example 1 is used for 10min, and the average killing logarithm value of colibacillus and staphylococcus aureus stained on the cloth piece is more than 3.00; meets the regulations of disinfection qualification of the Ministry of health, GB38502-2020 sterilizing effect inspection method in disinfectant laboratory and the technical requirement of low-temperature disinfectant sanitation safety evaluation (national defense office supervision letter No. 2020, No. 1062) laboratory microorganism killing tests.
According to the method of the test example 2, under the constant temperature condition of-20 ℃, the cold-temperature disinfection solution of the example 5 is used for 10min, and the average killing logarithm value of colibacillus and staphylococcus aureus stained on the cloth piece is more than 3.00; meets the regulations of disinfection qualification of the Ministry of health, GB38502-2020 sterilizing effect inspection method in disinfectant laboratory and the technical requirement of low-temperature disinfectant sanitation safety evaluation (national defense office supervision letter No. 2020, No. 1062) laboratory microorganism killing tests.
According to the method of the test example 2, the cold-temperature disinfection solution of the example 2 is adopted for spraying for 10min at the temperature of minus 10 ℃, the average killing logarithm value of escherichia coli dyed on kraft paper is 5.73, and the killing logarithm value of each test is more than 3.00; meets the requirements of on-site tests of 'disinfection technical Specification' of the ministry of health and 'technical requirement for evaluating the sanitation and safety of low-temperature disinfectant'.
According to the method of the test example 2, the cold-temperature disinfection solution of the example 2 is adopted for spraying for 10min at the temperature of minus 10 ℃, the average killing logarithm value of staphylococcus aureus dyed on kraft paper is 5.48, and the killing logarithm value of each test is more than 3.00; meets the requirements of on-site tests of 'disinfection technical Specification' of the ministry of health and 'technical requirement for evaluating the sanitation and safety of low-temperature disinfectant'.
According to the method of the test example 2, the cold-temperature disinfection solution of the example 3 is adopted for spraying for 10min at the temperature of 20 ℃ below zero, the average killing logarithm value of escherichia coli dyed on kraft paper is 5.82, and the killing logarithm value of each test is more than 3.00; meets the requirements of on-site tests of 'disinfection technical Specification' of the ministry of health and 'technical requirement for evaluating the sanitation and safety of low-temperature disinfectant'.
According to the method of the test example 2, the cold-temperature disinfection solution of the example 4 is adopted for spraying for 10min at the temperature of 20 ℃ below zero, the average killing logarithm value of staphylococcus aureus dyed on kraft paper is 5.76, and the killing logarithm value of each test is more than 3.00; meets the requirements of on-site tests of 'disinfection technical Specification' of the ministry of health and 'technical requirement for evaluating the sanitation and safety of low-temperature disinfectant'.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (6)
1. A cold-temperature disinfection solution is characterized by comprising a disinfection solution, a disinfection auxiliary agent and water;
the disinfectant comprises the following raw materials in percentage by mass: 4-6% of peroxyacetic acid, 20-30% of hydrogen peroxide and the balance of water;
the disinfection auxiliary agent comprises the following raw materials in percentage by mass: 30% of glycerol and 70% of 1-3 propylene glycol.
2. The cold-warm disinfection solution as claimed in claim 1, wherein the mass ratio of the disinfection solution to the disinfection auxiliary to the water is 1:0.3-1: 50-100.
3. The preparation method of the cold-temperature disinfection solution is characterized by comprising the following steps:
(1) weighing the raw materials according to the dosage of claim 1 or 2;
(2) diluting the disinfectant with water at low temperature;
(3) and adding the disinfection auxiliary agent into the diluted disinfection solution, and uniformly stirring to obtain the cold-warm disinfection solution.
4. The method of claim 3, wherein the low temperature condition in step (2) is-20 ℃ to 10 ℃.
5. The method of claim 3, wherein the stirring speed in step (3) is 100r/min, and the stirring time is 10-15 min.
6. The method for using cold-warm disinfectant, which is characterized in that the cold-warm disinfectant as described in claim 1 or 2 is used, and the cold-warm disinfectant is atomized and sprayed by a spraying locomotive, wherein the amount of the liquid medicine sprayed per square meter is 35ml to 40 ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110845550.3A CN113545341A (en) | 2021-07-26 | 2021-07-26 | Cold-temperature disinfectant and preparation method and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110845550.3A CN113545341A (en) | 2021-07-26 | 2021-07-26 | Cold-temperature disinfectant and preparation method and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113545341A true CN113545341A (en) | 2021-10-26 |
Family
ID=78132826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110845550.3A Pending CN113545341A (en) | 2021-07-26 | 2021-07-26 | Cold-temperature disinfectant and preparation method and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113545341A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0289463A1 (en) * | 1987-05-01 | 1988-11-02 | Ciba-Geigy Ag | Stabilized hydrogen peroxide solution |
| EP0953283A1 (en) * | 1998-04-30 | 1999-11-03 | Farmec S.n.c. di Tabasso Renato & C. | Method for production on the spot of a disinfectant peracetic acid system |
| JP2006193631A (en) * | 2005-01-14 | 2006-07-27 | Yokohama Yushi Kogyo Kk | Pasteurizing and cleaning composition containing peracetic acid and nonionic surfactant |
| CN101053330A (en) * | 2006-04-12 | 2007-10-17 | 拉萨绿能科技实业有限公司 | Disinfectant for improving hydrogen peroxide bactericidal activity |
| CN102273488A (en) * | 2011-06-22 | 2011-12-14 | 李翰实 | Nascent oxygen high speed specific bactericidal disinfectant |
| CN103704264A (en) * | 2014-01-09 | 2014-04-09 | 南通思锐生物科技有限公司 | Disinfecting detergent |
| CN107950568A (en) * | 2017-10-25 | 2018-04-24 | 中国人民解放军陆军防化学院 | Sanitizer formulations, application method and application |
| CN110150279A (en) * | 2019-06-03 | 2019-08-23 | 南通思锐生物科技有限公司 | A kind of formula and method quickly generating peroxyacetic acid disinfectant immediately |
| CN111388339A (en) * | 2020-03-27 | 2020-07-10 | 陈乐� | Novel quick washing-free disinfectant |
| CN112335657A (en) * | 2020-11-09 | 2021-02-09 | 史瑞雪 | Low-temperature-resistant low-corrosion-resistant peroxyacetic acid disinfectant and preparation method thereof |
| CN112352780A (en) * | 2020-10-19 | 2021-02-12 | 南京市产品质量监督检验院 | Isolated air purification disinfectant |
| CN112790191A (en) * | 2021-01-06 | 2021-05-14 | 海韵一剑大卫生科技有限公司 | Low-temperature peracetic acid disinfectant and preparation method thereof |
-
2021
- 2021-07-26 CN CN202110845550.3A patent/CN113545341A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0289463A1 (en) * | 1987-05-01 | 1988-11-02 | Ciba-Geigy Ag | Stabilized hydrogen peroxide solution |
| EP0953283A1 (en) * | 1998-04-30 | 1999-11-03 | Farmec S.n.c. di Tabasso Renato & C. | Method for production on the spot of a disinfectant peracetic acid system |
| JP2006193631A (en) * | 2005-01-14 | 2006-07-27 | Yokohama Yushi Kogyo Kk | Pasteurizing and cleaning composition containing peracetic acid and nonionic surfactant |
| CN101053330A (en) * | 2006-04-12 | 2007-10-17 | 拉萨绿能科技实业有限公司 | Disinfectant for improving hydrogen peroxide bactericidal activity |
| CN102273488A (en) * | 2011-06-22 | 2011-12-14 | 李翰实 | Nascent oxygen high speed specific bactericidal disinfectant |
| CN103704264A (en) * | 2014-01-09 | 2014-04-09 | 南通思锐生物科技有限公司 | Disinfecting detergent |
| CN107950568A (en) * | 2017-10-25 | 2018-04-24 | 中国人民解放军陆军防化学院 | Sanitizer formulations, application method and application |
| CN110150279A (en) * | 2019-06-03 | 2019-08-23 | 南通思锐生物科技有限公司 | A kind of formula and method quickly generating peroxyacetic acid disinfectant immediately |
| CN111388339A (en) * | 2020-03-27 | 2020-07-10 | 陈乐� | Novel quick washing-free disinfectant |
| CN112352780A (en) * | 2020-10-19 | 2021-02-12 | 南京市产品质量监督检验院 | Isolated air purification disinfectant |
| CN112335657A (en) * | 2020-11-09 | 2021-02-09 | 史瑞雪 | Low-temperature-resistant low-corrosion-resistant peroxyacetic acid disinfectant and preparation method thereof |
| CN112790191A (en) * | 2021-01-06 | 2021-05-14 | 海韵一剑大卫生科技有限公司 | Low-temperature peracetic acid disinfectant and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 杨光辉等: "化学物质与杀菌、消毒", 《化学教育》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112704076B (en) | Low-temperature disinfectant and preparation and application thereof | |
| CN112825851B (en) | High-stability 84 disinfectant with corrosion inhibition effect and preparation method thereof | |
| CN101766552A (en) | Washing-free antibacterial hand sanitizer and preparation method thereof | |
| CN112753699B (en) | Low-temperature chlorine-containing disinfectant and preparation and application thereof | |
| CN112741087A (en) | Cold chain food disinfectant and preparation method thereof | |
| CN112022732A (en) | Hand-washing-free disinfectant and preparation method thereof | |
| CN112790191A (en) | Low-temperature peracetic acid disinfectant and preparation method thereof | |
| CN113545341A (en) | Cold-temperature disinfectant and preparation method and application method thereof | |
| CN113016798A (en) | Low-temperature bactericide | |
| Bartlett et al. | Surfactant-iodine complexes as germicides | |
| Bezanson et al. | Effect of composting on microbial contamination and quality of fresh fruits and vegetables-a mini-review | |
| CN101646340B (en) | Production of a viable, storable worm egg suspension | |
| Thornley | Microbiological aspects of the use of radiation for the elimination of Salmonellae from foods and feeding stuffs | |
| CN113632803B (en) | Quaternary ammonium salt complex iodine disinfectant and preparation method thereof | |
| CN107177045B (en) | Collagen-lysozyme antibacterial film and preparation method thereof | |
| CN114698634A (en) | Application of isomeric tridecanol polyoxyethylene ether in iodine disinfectant and application method thereof | |
| CN110800754A (en) | Compound preparation for preventing and treating peach bacterial perforation disease and application | |
| CN114631529B (en) | Unitary stable composite peroxyacetic acid disinfectant and preparation method thereof | |
| CN112293707A (en) | Ozone treatment method for sterilizing kiwi fruit pollen canker | |
| CN110024785A (en) | It is a kind of for preventing and treating the fungicide of wilt disease | |
| CN112970776B (en) | Low-temperature disinfectant for object surface, preparation method and application thereof | |
| DEMCHICK et al. | INFLUENCE OF pH ON FREEZE‐THAW LETHALITY IN STAPHYLOCOCCUS AUREUS | |
| Landeka et al. | Determination of honey quality in the context of physico-chemical and microbiological data in Bosnia and Herzegovina | |
| Fan et al. | Effect of negative air ions on Escherichia coli ATCC 25922 inoculated onto mung bean seed and apple fruit | |
| Maktabi et al. | Decontamination of Salmonella Typhimurium and Listeria monocytogenes on Food-Related Surfaces by a Combination of Sodium Dodecyl Sulfate, Lactic Acid, or Citric Acid Under Different Temperatures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211026 |
|
| RJ01 | Rejection of invention patent application after publication |