CN113528308A - 紫外光谱液体活检rna.dna表型检测仪 - Google Patents
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Abstract
病毒流行时与普通感冒的RNA表观质量是不同，病毒的发热量是不同，我们可以使用温度，光谱吸收波长曲线来测定它们，紫外光谱液体活检RNA表型检测仪原理是采用表观电子光谱液体活检技术，能检测出新冠病毒RNA表观信息变化，纵多表观质量增加会使电子的能级发生变化引起电子光谱谱线位移，吸收比值变化，紫外光谱液体活检RNA表型检测仪技术(新冠病毒RNA二维表观质量光谱检测)，匹配新冠病毒核酸一维测序PCR检测，更加完善了病毒基因检测技木。
Description
技术领域
建立针对新冠病毒的检测方法目前有三类：病毒核酸检测、病毒抗原检测以及病毒抗体检测。其中，核酸一维测序检测是目前最可靠的筛查和确认手段，使用也最为普遍。而核酸检测方法又分为两类-基因法和实时荧光定量PCR(RT- PCR)法。作为诊断金标准的核酸检测，临床反映其阳性率只有30％-50％，采样难度所造成的误差应是主因。这将可能导致假阴性的患者提前出院，进而重新成为传染源。血清学诊断，主要是检测患者血清中的抗体水平，即用已知的流行病毒抗原来检测血清中的抗体。血清抗体检测方法不能在感染初期抗体还未产生或低于检测浓度时进行检测。通常在在新冠病毒发病7d后逐渐升高时才能检测抗体，故不能达到有效快速的诊断方法。如何能快速鉴别它是普通细菌肺炎还是新冠病毒肺炎，这种急需的核酸检测设备被提上了议事日程。
背景技术
新冠病毒基因二维表观检测技术异军突起。普通感冒大多是着凉之后出现，可能是有病毒感染引起，包括流感病毒、副流感病毒等，也可能是由细菌感染导致，常见的包括有肺炎链球菌感染等。普通感冒系细菌感染与病毒流行衰弱后RNA所导致，与现在流行期间恶性病毒感染的RNA表观质量是不相同的，因此很容易通过它们的表观质量我们来快速鉴别。新冠病毒流行时与新冠病毒衰减后期的RNA表观质量是不同，病毒的发热量是不同。对此恶性病毒我们可以使用温度，光谱吸收波长曲线来测定它们。这与元素同位素一样，流行时恶性病毒会因RNA表观异质性的不同，而引起光谱线的位移，(病毒光谱同位素效应)。鉴别普通感冒.新冠病毒表型分析仪作用原理是采用表观电子光谱“液体活检”技术，能检测出新冠病毒RNA核异质表观信息变化，(新冠病毒S蛋白、糖蛋白，唾液酸基的质量增加，RNA、蛋白质甲基化等表观质量增加)，纵多表观质量增加会使电子的能级发生变化，引起电子光谱或分子光谱的谱线位移。能引起光谱精细结构变化。“表观液体活检”诊断技术无论从基础理论上还是技木层面上都呈现其优越性。使得新冠病毒RNA、DNA(广谱)常规表观质量检测，匹配新冠病毒核酸一维测序PCR检测，更加完善了病毒基因检测。病毒光谱分析能够异军突起作为病毒早期筛查诊断技术，逐渐从科研走向临床。因此新冠病毒基因二维检测技术，应是目前这种急需的核酸检测设备，更值得引起我们的高度关注与重视。
发明内容
紫外分光光度计检测DNA或蛋白质进行定量定性分析而著称。是医学液体活检不可或缺的设备。紫外光谱仪是国际国内认可，技术成熟的DNA或蛋白质定量分析计量器具。医院实验室常用紫外分光光度计一维紫外光谱图测定(普通的紫外光谱图)进行常规核酸蛋白定量分析。在紫外液体活检RNA.DNA表型检检测仪中，我们则是利用紫外分光光度计二维技术来扩展它的应用范围，利用紫外吸收光谱测能技术(二维紫外光谱图)，来检测新冠病毒RNA表观电子特异性，可广泛用于流行期间恶性病毒普遍筛检。
紫外液体活检RNA.DNA表型检测仪，是根据爱因斯坦*测量一个物体的质量就是测量其中的能量原理。利用紫外光谱检测新冠病毒的复制动量就是在检测病毒基因二维的表观质量为基础理论。我们利用紫外吸收光谱测能技术(二维紫外光谱图)利用DNA含氢基团(OH、NH、CH)基态以及激发振动吸收情况，来检测病毒具有不同的能量曲线和平衡核间距。来建立紫外普通感冒.病毒流感表型分析仪。二维紫外光谱检测病毒，肿瘤应用十分广泛。能解决一些我们用常规方法所不易观测的病毒表观现象。揭示了新冠病毒RNA一维光谱中被掩盖的信息量，如可观测新冠病毒RNA高级构象变化，分子空间结构信息，分子间的相互作用问题，
这些微观层面的现象理解都可以通过病毒二维紫外的方法测得。紫外表型分析仪能进行常规病毒核酸蛋白分析，又能进行病毒.肿瘤早期诊断及其预后治疗，
一机专用也能一机多用。紫外普通.病毒流感表型分析仪检测病毒核异质RNA或蛋白质定量定性质量灵敏可靠。很少出现假阳性。更适合检测病毒的表观质量生物学指标。通过简单的一滴血，检测一滴唾液快速区分普通.流行病毒表型分析。来减少侵入性和昂贵的血清抗体检测诊断和监控流程这对于流行病毒的防预而言是一个里程碑。
具体实施方式
1，紫外液体活检RNA.DNA表型检监测仪检测方法，紫外普通感冒.病毒流感表型分析，一滴血快速全血法，血清法。
仪器组成：仪器主要由光源、单色器、样品室、检测器、信号处理器和显示与存储系统组成。采用一个可以产生多个波长的光源，通过系列分光装置，从而产生特定波长的光源，光线透过测试的样品后，部分光线被吸收，计算样品的吸光值，从而转化成样品的浓度。样品的吸光值与样品的浓度成正比。
紫外液体活检RNA.DNA表型检测仪。仪器参数设定：测试范围为紫外波段200nm～400nm，采样间隔0.5rim，狭缝宽度5.0nto，中速自动扫描，检测器为光电倍增管。吸收光谱采集：在样品池和参比池中，分别注入3ml的蒸馏水做基线校准，然后用微量取液器取0.2ml血清稀释至3ml注入样品池，按选定的实验条件测量。3.1血清样品紫外吸收谱实验
首先分析人体血清的紫外吸收光谱。参比池中为3ml蒸馏水，
样品池中为2.8ml蒸馏水+0.2ml血清时得到的吸收谱表现的是血清的信息。
血常规光谱紫外液体活检RNA.DNA表型检测仪检测方法分别研究了健康人、病毒，肿瘤患者血液、血清、淋巴液等细胞光谱同位素特性。研究结果表明，人血清的紫外吸收谱约在230nm附近有一极强的吸收峰，血液在275nm，
血清在279nm有一较强的吸收峰，在252nm有吸收谷。分析血清表明，病毒流感与癌生物同位素光谱效应；紫外吸收谱有差异。冠状病毒，癌患者波谷位置平均值252.5-253nm，健康者波谷位置的平均值252(P＜0.01)。(符合率88.2 ％)而健康者应小于253nm(符合率81.8％)。
假阴性可能受采样人员技术、疾病本身间断性排毒等因素的影响。核酸检测的次数越多就越保险，但也需要考虑可行性。一滴血病毒核酸快速全血法在这时尤其显得十分的重要。通过简单的一滴血，时时监控复查，尽可能避免极少数病人在恢复过程中复阳造成传染的可能风险。值得提出的是虽然紫外病毒流感表型分析仪一滴血快速全血法，一滴唾液分析法特异性，敏感性很高，流感病毒，癌症早期诊断筛检十分的重要。配合绿色健康证来，则更加完美，患者健康时注册血液.血清.唾液健康时的吸收峰谷值，病毒流行期间恶性特异性就表现出来了。对于预防病毒流行，保护癌患者早期诊断是很重要的。，
2，紫外液体活检RNA.DNA表型检测仪一滴唾液法，
新冠病毒者唾液是由三对大唾液腺(下颌腺、腮腺和舌下腺)分泌的液体PH值为6.6-7.1。唾液淀含有粉酶、黏多糖、黏蛋白、溶菌酶等。紫外普通感冒. 病毒流感表型分析仪采用病毒与肿瘤生物同位素效应分析法.一滴唾液快速监查法分别研究了健康人、健康人一滴唾液283nm-286nm有一较强吸收峰、在25 6nm-260nm有吸收谷。冠状病毒.肿瘤患者一滴唾液在255.84nm-255nm有一较强的吸收峰谷。是病毒与肿瘤患者RNA.DNA甲基化的重要贡献。些光谱差异对于病毒流感.癌光谱早期诊断及肿瘤治疗预后的定性、定量分析上，具有重要的理论价值及其临床筛检应用价值。液体活检唾液PH值随人群的分泌因素变化很大，检测时应固定PH值(如10mM Tris-HCl pH 8.0)，才能得到精确的检测结果。，紫外吸收光谱因人群有差异检测也时应配合健康时的吸收波谷位置平均值256nm-260nm吸收谷(P＜0.01)进行检测。
3，紫外液体活检RNA.DNA表型检测仪配合新冠病毒咽拭子检测率更优秀更准确常规对疑似患者进行两次咽拭子核酸检测，如果出现一次阳性则确诊，如出现两次阴性，则基本可排除感染。三次咽拭子新冠病毒核酸检测均为阴性，说明病毒隐匿性很强。有研究显示少数患者的感染部位仅限下呼吸道，而不在鼻咽部，所以咽拭子反复检测都是阴性，而CT显示症状不断加重的情况是存在的，对于这种特殊患者，我们建议取下呼吸道的肺泡灌洗液用来做冠状病毒核酸检测。而现在很难做到对每一例疑似患者都进行下呼吸道标本采样，这也给确诊带来一定挑战。在临床诊断中，除核酸检测之外，需进行流行病学史详细调查、观察肺部影像表现等综合来判断，提高诊断的准确率。对于高度疑似但核酸检测呈阴性的患者，这时如果有紫外液体活检RNA表型检测仪配合新冠病毒咽拭子检测率更优秀更准确。冠状病毒咽拭子液体活检RNA在255.84nm-255 nm有一特异性较强的吸收峰谷，阳性符合率80％。在咽拭子检测核酸一维测序检测基础上加强核酸二维光谱检测准确率可达符合率90％。
紫外液体活检表型分析仪技术是介入病毒核酸检测荧光定量RT-PCR，CT影像学之间的病毒基因二维表观检测技术。它的出现将添补新冠病毒检测法，使流行病毒筛检从红外测温，一滴血一滴唾液，病毒咽拭子紫外液体活检表型检监测仪技术筛检开始，然后CT影像学病灶的精确解剖定位。病毒核酸荧光定量PCR (RT-PCR)确诊检测，病毒抗体追综检测等。完善着整个新冠病毒的早期检测诊断。
Claims (3)
1.新冠病毒流行时与普通感冒的RNA表观质量与发热量是不同，我们可以使用温度.光谱吸收曲线来测定它们，紫外光谱液体活检RNA.DNA表型检测仪技术.能检测出新冠病毒RNA表观信息变化，紫外光谱RNA，DNA表型分析仪具有独特的紫外光谱RNA表型分析软件.科学基础理论以及专利技术所组成，任何紫外分光光度计不得在此基础上升级为紫外光谱液体活检RNA.DNA表型分析仪，打紫外光谱液体活检RNA(DNA)表型分析仪器牌号.对紫外光谱RNA(DNA)表型分析仪器进行注册生产的都属权利范围。
2.紫外光谱液体活检RNA表型检测仪为医疗器械是紫外分光光度计+紫外光谱液体活检RNA表型分析软件技术以及理论所组成的医疗器械，紫外吸收光谱测能技术(二维紫外光谱图)利用RNA含氢基团激发振动吸收情况，来检测RNA病毒具有不同的能量曲线，体现出新冠病毒RNA二维表观质量光谱检测等优势，紫外光谱液体活检RNA表型检测仪匹配新冠病毒核酸一维测序PCR检测，更加完善了病毒基因检测技木，利用紫外二维光谱信息进行液体活检病毒RNADNA表型分析应用是以赢利为目的也应从属权利要求。
3.紫外液体活检RNA.DNA表型检测仪，是根据爱因斯坦测量一个物体的质量就是测量其中的能量原理，利用紫外光谱检测新冠病毒的复制动量就是在检测病毒RNA二维的表观质量为基本理论，新冠病毒RNA二维检测仪器技术，是目前急需的核酸防控检测设备，能快速捕获到新冠病毒.SARS病毒RNA表型光谱特异信息号.广泛用于流行病毒RNA的检测防控与治疗，能在疫情早期防控上取得成功，冠状病毒二维紫外光谱快速检测一滴血.一滴唾液法，咽拭子液体活检RNA.DNA定量测定.表型分析等，能做到病毒RNA的早期防控，紫外液体活检表型分析仪技术是介入病毒核酸检测荧光定量RT-PCR，CT影像学之间的病毒基因二维表观光谱仪检测技术，它的出现将添补新冠病毒检测法，其仪器核心理论技术，根据权利要求请求保护。
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