CN113527088B - Method for extracting sodium lactate from nisin waste liquid - Google Patents
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- CN113527088B CN113527088B CN202110820618.2A CN202110820618A CN113527088B CN 113527088 B CN113527088 B CN 113527088B CN 202110820618 A CN202110820618 A CN 202110820618A CN 113527088 B CN113527088 B CN 113527088B
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- 239000007788 liquid Substances 0.000 title claims abstract description 55
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 239000001540 sodium lactate Substances 0.000 title claims abstract description 45
- 229940005581 sodium lactate Drugs 0.000 title claims abstract description 45
- 235000011088 sodium lactate Nutrition 0.000 title claims abstract description 45
- 239000002699 waste material Substances 0.000 title claims abstract description 44
- 108010053775 Nisin Proteins 0.000 title claims abstract description 30
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 title claims abstract description 30
- 239000004309 nisin Substances 0.000 title claims abstract description 30
- 235000010297 nisin Nutrition 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 18
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 43
- 238000001914 filtration Methods 0.000 claims abstract description 30
- 238000004042 decolorization Methods 0.000 claims abstract description 26
- 239000000047 product Substances 0.000 claims abstract description 25
- 239000003480 eluent Substances 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 14
- 238000001728 nano-filtration Methods 0.000 claims abstract description 14
- 239000012466 permeate Substances 0.000 claims abstract description 14
- 150000001450 anions Chemical class 0.000 claims abstract description 13
- 239000011347 resin Substances 0.000 claims abstract description 13
- 229920005989 resin Polymers 0.000 claims abstract description 13
- 238000007865 diluting Methods 0.000 claims abstract description 7
- 239000003085 diluting agent Substances 0.000 claims abstract description 7
- 239000008213 purified water Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 65
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 28
- 239000011780 sodium chloride Substances 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 13
- 239000003957 anion exchange resin Substances 0.000 claims description 12
- 238000001704 evaporation Methods 0.000 claims description 11
- 230000008020 evaporation Effects 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 10
- 238000010790 dilution Methods 0.000 claims description 6
- 239000012895 dilution Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000011049 filling Methods 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000007613 environmental effect Effects 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 5
- 238000000746 purification Methods 0.000 abstract description 4
- 239000002351 wastewater Substances 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 description 19
- 235000013305 food Nutrition 0.000 description 6
- 241000194035 Lactococcus lactis Species 0.000 description 2
- 235000014897 Streptococcus lactis Nutrition 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
- C07C51/44—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation by distillation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Crystallography & Structural Chemistry (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention relates to the field of biochemical separation engineering, in particular to a method for extracting sodium lactate from nisin waste liquid. The method comprises the following steps: (1) Filtering nisin waste liquid by using a nanofiltration membrane to obtain a permeate; (2) Concentrating the permeate by using a multi-effect evaporator, and centrifuging to obtain concentrated solution; (3) Decolorizing the concentrated solution by using activated carbon, and filtering to obtain primary decolorized solution; (4) Diluting the primary concentrated solution by using purified water to obtain a diluted solution; (5) Enriching and analyzing sodium lactate in the diluent by using anion resin; (6) Concentrating the eluent by using an MVR evaporator to obtain secondary concentrated solution; (7) And (3) performing secondary decolorization on the secondary concentrated solution by using activated carbon to obtain a qualified finished product. According to the invention, by designing the purification process of the nisin waste liquid, the sodium lactate finished product is extracted from the nisin waste liquid, so that the secondary utilization of waste water is realized, and the environmental protection pressure is reduced.
Description
Technical Field
The invention relates to the field of biochemical separation engineering, in particular to a method for extracting sodium lactate from nisin waste liquid.
Background
Nisin is a polypeptide antibacterial substance extracted from fermentation products of lactococcus lactis, and is a world-recognized safe and natural biological food preservative and antibacterial agent. 3% -8% of lactic acid can be produced in the process of producing nisin by fermentation of lactococcus lactis, sodium lactate can be obtained by purification, and the sodium lactate has wide industrial application and is widely applied to fresh-keeping, moisture-preserving, aroma-enhancing and pharmaceutical raw materials of foods.
The existing treatment mode is directly discharged into an environmental protection system for treatment, which is not only a waste of resources, but also brings great pressure to the environmental protection system. The process route designed by the invention not only obtains good environmental benefits, but also brings economic benefits to the development of enterprises, and increases the product category and profit point of the enterprises.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for extracting sodium lactate from nisin waste liquid, which extracts sodium lactate finished product from nisin waste liquid by designing a purification process of nisin waste liquid, realizes secondary utilization of waste water and reduces environmental protection pressure.
The technical scheme of the invention is as follows:
a method for extracting sodium lactate from nisin waste liquid, which comprises the following steps:
(1) Filtering waste liquid generated in the nisin extraction process by using a nanofiltration membrane to obtain a permeate;
(2) Concentrating the permeate by using a multi-effect evaporator, and centrifuging to obtain concentrated solution;
(3) Decolorizing the concentrated solution by using activated carbon, and filtering to obtain primary decolorized solution;
(4) Diluting the primary concentrated solution by using purified water to obtain a diluted solution;
(5) Enriching sodium lactate in the diluent by using anion resin, and obtaining eluent after analysis;
(6) Concentrating the eluent by using an MVR evaporator to obtain secondary concentrated solution;
(7) And (3) performing secondary decolorization on the secondary concentrated solution by using activated carbon to obtain a qualified finished product.
Preferably, in the step (1), liquid sodium hydroxide is firstly used for adjusting the pH value of the waste liquid to 5.0-7.0, and the nanofiltration membrane used in the filtration has a molecular weight cut-off of 200Da-1000Da. The nanofiltration membrane can well remove organic matters and chromaticity in the waste liquid and remove substances such as partial soluble salts.
Preferably, the multiple-effect evaporator used in the step (2) comprises a two-effect evaporator or a three-effect evaporator or a four-effect evaporator or an MVR evaporator, the evaporation temperature of the evaporator is 65-95 ℃, the concentration multiple of the evaporator is 5-15 times, after the concentration is finished, a centrifugal machine is used for centrifugal treatment, and solid sodium chloride in the concentrated solution is centrifugally separated to obtain solid sodium chloride and supernatant concentrated solution respectively.
Preferably, the addition amount of the activated carbon in the step (3) is 1% -5% (W/V), the temperature is 65-85 ℃, the decoloring time is 60 minutes, and the primary decoloring liquid is obtained by plate-frame filtration.
Preferably, the dilution factor of the step (4) is 1-fold or 2-fold or 3-fold.
Preferably, the anion resin used in the step (5) is a weak base anion exchange resin or a macroporous weak base anion exchange resin, and the resolving agent used is 0.1M-5M sodium hydroxide solution.
Preferably, the concentration multiple of the step (6) is 3-5 times, the evaporation temperature is 65-95 ℃, and the final content of sodium lactate reaches about 65%.
Preferably, the addition amount of the activated carbon in the step (7) is 1% -5% (W/V), the temperature is 65 ℃ -85 ℃, and the decoloring treatment time is 60 minutes.
Preferably, the activated carbon is filtered by a plate frame after the decolorization in the step (7), the temperature is controlled between 50 ℃ and 60 ℃ during the filtration, clear plate liquid is obtained after the filtration, and the finished product is obtained after the temperature is reduced to normal temperature and is filled by a food-grade container.
The beneficial effects of the invention are that
According to the invention, the sodium lactate finished product is extracted from the nisin waste liquid by optimizing the flow of the waste liquid extraction process and designing the nisin waste liquid purification process, so that the purposes of secondary production and utilization and environmental protection are achieved.
1) According to the invention, sodium lactate in the waste liquid is extracted, so that COD of the waste liquid is reduced, and the environment is effectively protected.
2) In the process of extracting sodium lactate, the solid sodium chloride byproduct can be recycled, the content of sodium chloride in the waste liquid is effectively reduced, the disposability of the waste liquid is improved, and the environmental benefit is further improved.
3) According to the invention, waste is recycled, waste is changed into valuable, and the food additive product sodium lactate is obtained, so that economic benefit is obtained.
Detailed Description
In order to better understand the technical solutions of the present invention, the following description will clearly and completely describe the technical solutions of the embodiments of the present invention in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Example 1
A method for extracting sodium lactate from nisin waste liquid, which comprises the following steps:
(1) The pH value of the waste liquid is adjusted to 5.0-7.0 by using liquid sodium hydroxide, then the waste liquid generated in the nisin extraction process is filtered by using a nanofiltration membrane, the molecular weight cut-off of the nanofiltration membrane used in the filtration is 200Da-1000Da, and then a permeate is obtained;
(2) Concentrating the permeate by using a multi-effect evaporator, wherein the used multi-effect evaporator is a double-effect evaporator, the evaporation temperature of the evaporator is 65-95 ℃, the concentration multiple of the evaporator is 10 times, after the concentration is finished, the concentrated solution is subjected to centrifugal treatment by using a centrifugal machine, and then solid sodium chloride in the concentrated solution is subjected to centrifugal separation to obtain solid sodium chloride and supernatant concentrated solution respectively;
(3) Decolorizing the concentrated solution by using active carbon with the addition amount of 1% (W/V) and the temperature of 65-85 ℃, and filtering by using a plate frame after 60 minutes of decolorizing treatment to obtain primary decolorized solution;
(4) Diluting the primary concentrated solution by using purified water to obtain a diluted solution; the dilution factor is 2 times;
(5) Enriching sodium lactate in the diluent by using anion resin, wherein the anion resin adopts weak alkaline anion exchange resin or macroporous weak alkaline anion exchange resin; resolving, namely resolving the eluent into 0.1M sodium hydroxide solution;
(6) Concentrating the eluent by using an MVR evaporator, wherein the concentration multiple is 3-5 times, the evaporation temperature is 65-95 ℃ until the final content of sodium lactate reaches about 65%, and obtaining secondary concentrated solution;
(7) And (3) performing secondary decolorization on the secondary concentrated solution by using active carbon, wherein the addition amount of the active carbon is 3% (W/V), the decolorization temperature is 65-85 ℃, the decolorization treatment is performed for 60 minutes, the active carbon is filtered by using a plate frame after the decolorization is finished, the temperature is controlled to be 50-60 ℃ during the filtration, the clear plate liquid is obtained after the filtration, and the finished product is obtained after the temperature is reduced to normal temperature and the finished product is obtained by filling the food-grade container.
The yield of the sodium lactate extracted by the embodiment is 75.5%, and the product quality reaches the national food safety standard of sodium lactate.
Example 2
A method for extracting sodium lactate from nisin waste liquid, which comprises the following steps:
(1) The pH value of the waste liquid is adjusted to 5.0-7.0 by using liquid sodium hydroxide, then the waste liquid generated in the nisin extraction process is filtered by using a nanofiltration membrane, the molecular weight cut-off of the nanofiltration membrane used in the filtration is 200Da-1000Da, and then a permeate is obtained;
(2) Concentrating the permeate by using a multi-effect evaporator, wherein the used multi-effect evaporator is a three-effect evaporator, the evaporation temperature of the evaporator is 65-95 ℃, the concentration multiple of the evaporator is 15 times, after the concentration is finished, the concentrated solution is subjected to centrifugal treatment by using a centrifugal machine, and then solid sodium chloride in the concentrated solution is subjected to centrifugal separation to obtain solid sodium chloride and supernatant concentrated solution respectively;
(3) Decolorizing the concentrated solution by using active carbon, wherein the addition amount of the active carbon is 5% (W/V), the temperature is 65-85 ℃, and the primary decolorized solution is obtained by filtering the concentrated solution by using a plate frame after 60 minutes of decolorization treatment;
(4) Diluting the primary concentrated solution by using purified water to obtain a diluted solution; the dilution factor is 3 times;
(5) Enriching sodium lactate in the diluent by using anion resin, wherein the anion resin adopts weak alkaline anion exchange resin or macroporous weak alkaline anion exchange resin; resolving, namely resolving the eluent into 2M sodium hydroxide solution, and obtaining eluent after resolving;
(6) Concentrating the eluent by using an MVR evaporator, wherein the concentration multiple is 3-5 times, the evaporation temperature is 65-95 ℃ until the final content of sodium lactate reaches about 65%, and obtaining secondary concentrated solution;
(7) And (3) carrying out secondary decolorization on the secondary concentrated solution by using active carbon, wherein the addition amount of the active carbon is 1% (W/V), the decolorization temperature is 65-85 ℃, the decolorization treatment is carried out for 60 minutes, the active carbon is filtered by using a plate frame after the decolorization is finished, the temperature is controlled to be 50-60 ℃ during the filtration, the clear plate liquid is obtained after the filtration, and the finished product is obtained after the temperature is reduced to normal temperature and the finished product is obtained by filling by using a food-grade container.
The yield of the sodium lactate extracted by the embodiment is 80.4 percent, and the product quality reaches the national food safety standard of sodium lactate.
Example 3
A method for extracting sodium lactate from nisin waste liquid, which comprises the following steps:
(1) The pH value of the waste liquid is adjusted to 5.0-7.0 by using liquid sodium hydroxide, then the waste liquid generated in the nisin extraction process is filtered by using a nanofiltration membrane, the molecular weight cut-off of the nanofiltration membrane used in the filtration is 200Da-1000Da, and then a permeate is obtained;
(2) Concentrating the permeate by using a multi-effect evaporator, wherein the multi-effect evaporator is an MVR evaporator, the evaporation temperature of the evaporator is 65-95 ℃, the concentration multiple of the evaporator is 8 times, after the concentration is finished, centrifuging the concentrated solution by using a centrifuge, and then centrifuging solid sodium chloride in the concentrated solution to obtain solid sodium chloride and supernatant concentrated solution respectively;
(3) Decolorizing the concentrated solution by using active carbon, wherein the addition amount of the active carbon is 3% (W/V), the temperature is 65-85 ℃, and the primary decolorized solution is obtained by plate-frame filtration after 60 minutes of decolorization treatment;
(4) Diluting the primary concentrated solution by using purified water to obtain a diluted solution; the dilution factor is 1 time;
(5) Enriching sodium lactate in the diluent by using anion resin, wherein the anion resin adopts weak alkaline anion exchange resin or macroporous weak alkaline anion exchange resin; resolving, namely resolving the eluent by using 5M sodium hydroxide solution;
(6) Concentrating the eluent by using an MVR evaporator, wherein the concentration multiple is 3-5 times, the evaporation temperature is 65-95 ℃ until the final content of sodium lactate reaches about 65%, and obtaining secondary concentrated solution;
(7) And (3) carrying out secondary decolorization on the secondary concentrated solution by using active carbon, wherein the addition amount of the active carbon is 5% (W/V), the decolorization temperature is 65-85 ℃, the decolorization treatment is carried out for 60 minutes, the active carbon is filtered by using a plate frame after the decolorization is finished, the temperature is controlled to be 50-60 ℃ during the filtration, the clear plate liquid is obtained after the filtration, and the finished product is obtained after the temperature is reduced to normal temperature and the finished product is obtained by filling by using a food-grade container.
The yield of the sodium lactate extracted by the embodiment is 78.4 percent, and the product quality reaches the national food safety standard of sodium lactate.
Example 4
A method for extracting sodium lactate from nisin waste liquid, which comprises the following steps:
(1) The pH value of the waste liquid is adjusted to 5.0-7.0 by using liquid sodium hydroxide, then the waste liquid generated in the nisin extraction process is filtered by using a nanofiltration membrane, the molecular weight cut-off of the nanofiltration membrane used in the filtration is 200Da-1000Da, and then a permeate is obtained;
(2) Concentrating the permeate by using a multi-effect evaporator, wherein the used multi-effect evaporator is a four-effect evaporator, the evaporation temperature of the evaporator is 65-95 ℃, the concentration multiple of the evaporator is 12 times, after the concentration is finished, the concentrated solution is subjected to centrifugal treatment by using a centrifugal machine, and then solid sodium chloride in the concentrated solution is subjected to centrifugal separation to obtain solid sodium chloride and supernatant concentrated solution respectively;
(3) Decolorizing the concentrated solution by using active carbon, wherein the addition amount of the active carbon is 4% (W/V), the temperature is 65-85 ℃, and the primary decolorized solution is obtained by filtering the concentrated solution by using a plate frame after 60 minutes of decolorization treatment;
(4) Diluting the primary concentrated solution by using purified water to obtain a diluted solution; the dilution factor is 3 times;
(5) Enriching sodium lactate in the diluent by using anion resin, wherein the anion resin adopts weak alkaline anion exchange resin or macroporous weak alkaline anion exchange resin; resolving, namely resolving the eluent into 3M sodium hydroxide solution, and obtaining eluent after resolving;
(6) Concentrating the eluent by using an MVR evaporator, wherein the concentration multiple is 3-5 times, the evaporation temperature is 65-95 ℃ until the final content of sodium lactate reaches about 65%, and obtaining secondary concentrated solution;
(7) And (3) performing secondary decolorization on the secondary concentrated solution by using active carbon, wherein the addition amount of the active carbon is 3% (W/V), the decolorization temperature is 65-85 ℃, the decolorization treatment is performed for 60 minutes, the active carbon is filtered by using a plate frame after the decolorization is finished, the temperature is controlled to be 50-60 ℃ during the filtration, the clear plate liquid is obtained after the filtration, and the finished product is obtained after the temperature is reduced to normal temperature and the finished product is obtained by filling the food-grade container.
The yield of the sodium lactate extracted by the embodiment is 83.5%, and the product quality reaches the national food safety standard of sodium lactate.
For examples 1-4 of the present application, some of the variable parameters were extracted and sorted to produce Table 1.
Table 1 summary of variable parameters of examples 1-4
The sodium lactate yield obtained after the secondary extraction is very high, generally more than 75%, and the quality of the product meets the standard requirements of national food safety standard sodium lactate (solution) of GB 25537-2010.
Examples 1-4 four groups of nisin waste liquid have COD of 30000-40000mg/L before secondary extraction and COD of 5000-8000mg/L after sodium lactate extraction, so that the COD of the waste liquid is obviously reduced and the environment is effectively protected.
Although the present invention has been described in detail by way of the preferred embodiments, the present invention is not limited thereto. Various equivalent modifications and substitutions may be made in the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and it is intended that all such modifications and substitutions be within the scope of the present invention/be within the scope of the present invention as defined by the appended claims. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (4)
1. A method for extracting sodium lactate from nisin waste liquid, which is characterized by comprising the following steps:
(1) Firstly, adjusting the pH value of the waste liquid to 5.0-7.0 by using liquid sodium hydroxide, filtering the waste liquid generated in the nisin extraction process by using a nanofiltration membrane, wherein the interception molecular weight of the nanofiltration membrane used in the filtration is 200Da-1000Da, and obtaining a permeate;
(2) Concentrating the permeate by using a multi-effect evaporator, and centrifuging to obtain concentrated solution; the multi-effect evaporator comprises a four-effect evaporator, the evaporation temperature of the evaporator is 65-95 ℃, the concentration multiple of the evaporator is 12 times, after the concentration is finished, a centrifugal machine is used for centrifugal treatment, and solid sodium chloride in the concentrated solution is centrifugally separated to respectively obtain solid sodium chloride and supernatant concentrated solution;
(3) Decolorizing the concentrated solution by using activated carbon, and filtering to obtain primary decolorized solution;
(4) Diluting the primary concentrated solution with purified water, wherein the dilution factor is 3 times, so as to obtain a diluted solution;
(5) Enriching sodium lactate in the diluent by using anion resin, and obtaining eluent after analysis; the anion resin is weak alkaline anion exchange resin or macroporous weak alkaline anion exchange resin, and the resolving agent is 0.1M-5M sodium hydroxide solution;
(6) Concentrating the eluent by using an MVR evaporator to obtain secondary concentrated solution;
(7) And (3) performing secondary decolorization on the secondary concentrated solution by using activated carbon to obtain a qualified finished product.
2. The method for extracting sodium lactate from nisin waste liquid according to claim 1, wherein the addition amount of the activated carbon in the step (3) is 1% -5% w/V, the temperature is 65 ℃ -85 ℃, and the primary decolorization liquid is obtained by plate-frame filtration after decolorization is completed.
3. The method for extracting sodium lactate from nisin waste liquid according to claim 1, wherein the activated carbon in the step (7) is added in an amount of 1% -5% w/V, the temperature is 65 ℃ -85 ℃, and the decoloring time is 60 minutes.
4. The method for extracting sodium lactate from nisin waste liquid according to claim 1, wherein the step (7) is characterized in that activated carbon is filtered by a plate frame after the decolorization is completed, the temperature is controlled between 50 ℃ and 60 ℃ during the filtration, clear plate liquid is obtained after the filtration, and the finished product is obtained after the temperature is reduced to normal temperature and then the finished product is obtained by filling the food-grade container.
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