CN113519417B - Luhai relay breeding method for oplegnathus punctatus - Google Patents
Luhai relay breeding method for oplegnathus punctatus Download PDFInfo
- Publication number
- CN113519417B CN113519417B CN202110792861.8A CN202110792861A CN113519417B CN 113519417 B CN113519417 B CN 113519417B CN 202110792861 A CN202110792861 A CN 202110792861A CN 113519417 B CN113519417 B CN 113519417B
- Authority
- CN
- China
- Prior art keywords
- oplegnathus
- transportation
- punctatus
- feed
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000353171 Oplegnathus punctatus Species 0.000 title claims abstract description 37
- 238000009395 breeding Methods 0.000 title description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 40
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000004471 Glycine Substances 0.000 claims abstract description 20
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims abstract description 20
- 239000004472 Lysine Substances 0.000 claims abstract description 20
- 229930182817 methionine Natural products 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 20
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims abstract description 18
- 239000003910 polypeptide antibiotic agent Substances 0.000 claims abstract description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 6
- 241000353172 Oplegnathus fasciatus Species 0.000 claims description 32
- 150000001413 amino acids Chemical group 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 241000353145 Oplegnathus Species 0.000 abstract description 13
- 230000037406 food intake Effects 0.000 abstract description 13
- 230000012010 growth Effects 0.000 abstract description 11
- 241000251468 Actinopterygii Species 0.000 abstract description 10
- 230000036039 immunity Effects 0.000 abstract description 4
- 238000012546 transfer Methods 0.000 abstract description 4
- 230000006978 adaptation Effects 0.000 abstract description 2
- 210000004185 liver Anatomy 0.000 description 11
- 230000032258 transport Effects 0.000 description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 7
- 239000003613 bile acid Substances 0.000 description 7
- 230000034994 death Effects 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 102000044503 Antimicrobial Peptides Human genes 0.000 description 4
- 108700042778 Antimicrobial Peptides Proteins 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 4
- 102000014150 Interferons Human genes 0.000 description 4
- 108010050904 Interferons Proteins 0.000 description 4
- 102000016943 Muramidase Human genes 0.000 description 4
- 108010014251 Muramidase Proteins 0.000 description 4
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 4
- 241001494106 Stenotomus chrysops Species 0.000 description 4
- 229930003268 Vitamin C Natural products 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 229940079322 interferon Drugs 0.000 description 4
- 235000010335 lysozyme Nutrition 0.000 description 4
- 229960000274 lysozyme Drugs 0.000 description 4
- 239000004325 lysozyme Substances 0.000 description 4
- 235000019154 vitamin C Nutrition 0.000 description 4
- 239000011718 vitamin C Substances 0.000 description 4
- 102000016938 Catalase Human genes 0.000 description 3
- 108010053835 Catalase Proteins 0.000 description 3
- 102000006587 Glutathione peroxidase Human genes 0.000 description 3
- 108700016172 Glutathione peroxidases Proteins 0.000 description 3
- 241000269808 Sparus Species 0.000 description 3
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000005228 liver tissue Anatomy 0.000 description 3
- 230000000242 pagocytic effect Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 241000235342 Saccharomycetes Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 241001519451 Abramis brama Species 0.000 description 1
- 241000607525 Aeromonas salmonicida Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000607471 Edwardsiella tarda Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000826789 Lutraria Species 0.000 description 1
- 241000269810 Pagrus Species 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241000544286 Vibrio anguillarum Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000002815 broth microdilution Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a method for relay culture of oplegnathus punctatus in the land and sea, which is characterized in that oplegnathus punctatus cultured in land-based industrial circulating water is transported to a net cage and/or a fence for culture by a live fish transport ship at the beginning of 6 months at the end of 5 months every year; and feeding the feed rich in glycine, lysine and methionine before the transfer. According to the invention, the feed rich in glycine, lysine and methionine is fed to the oplegnathus maculatus before transportation, so that the stress of transportation to the oplegnathus maculatus is effectively reduced, the adaptation time of the factory-cultured oplegnathus maculatus to the natural ecological environment after transportation is shortened, and the normal ingestion of the oplegnathus maculatus is promoted, so that the growth can be recovered more quickly, and meanwhile, the yeast capable of expressing the antibacterial peptide is added into the feed, so that the non-specific immunity of the oplegnathus maculatus can be improved, and the stress resistance to the environment is enhanced.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a rock bream terrestrial sea relay culture method.
Background
Oplegnathus punctatus (Oplegnathus punctatus) belongs to the fishes in the hot offshore region, is widely distributed in the peripheral sea areas such as the yellow sea, the east sea, the south sea, the Korean peninsula, the Japanese peninsula and the like in China, and has the advantages of fine and smooth meat quality, rich nutrition, high growth speed and the like. Meanwhile, the market price is stable at 160-200 yuan/kg, the economic benefit is remarkable, and the feed is popular with breeding manufacturers in Shandong, Jiangsu, Fujian and Guangdong coastal areas at present, and the industrialization prospect is wide.
Land-based industrial circulating water and deep open sea large-scale net cages/fences are two main modes for culturing the oplegnathus fasciatus at present. The land-based factory circulating water culture mode has the advantages of water conservation, land conservation, accurate regulation and control of environmental factors such as water temperature, dissolved oxygen and the like, can not be influenced by external environment, realizes annual culture, and maintains higher operation energy consumption cost of the whole culture system. And the large-scale net cages and fences in deep and open sea trap culture water is large in volume, the moving space of culture objects is large, the culture water is sufficient, the water exchange is sufficient, the utilization rate of residual baits is high, the natural endowment of sea areas is fully utilized, the growth speed is high, meanwhile, the quality is closer to natural ecology, but the dissolved oxygen of the culture water environment temperature is not artificially controlled, and the whole-year culture cannot be realized. The oplegnathus fasciatus belongs to warm-water marine fishes, the optimal growth temperature is 20-28 ℃, the growth of the oplegnathus fasciatus is inhibited at the temperature lower than 13 ℃, the oplegnathus fasciatus is cold in northern winter, the oplegnathus fasciatus cannot naturally overwinter in a culture sea area, and the oplegnathus fasciatus needs to be transferred to an industrial workshop for overwinter conservation. Therefore, how to increase the growth rate of the oplegnathus punctatus, shorten the cultivation period on the market, reduce the production energy consumption in the cultivation process, and obtain high-quality products becomes a core problem concerned by the cultivation enterprises at present.
Disclosure of Invention
The invention aims to provide a spot sea relay culture method for oplegnathus punctatus, namely a method for industrially circulating water and carrying out net cage and fence relay culture on the oplegnathus punctatus.
The method for relay culture of oplegnathus punctatus in upland sea provided by the invention is characterized in that oplegnathus punctatus cultured in land-based industrial circulating water is transported to a net cage and/or a fence for culture by a live fish transport ship at the beginning of 6 months at the end of 5 months every year; and feeding the feed rich in glycine, lysine and methionine before the transfer;
further, feeding feed rich in glycine, lysine and methionine 10-15 days before transportation;
the feed rich in glycine, lysine and methionine is prepared by adding glycine, lysine and methionine into Sparus punctatus feed;
further, yeast capable of expressing the antibacterial peptide is added into the feed;
a specific amino acid sequence of the antibacterial peptide is as follows (SEQ ID NO: 1): LGLDFKFKEKKSIGFKEKHIQVRIFKDLNLRVKTEWH, respectively;
a specific sequence of the nucleotide fragment encoding the above antibacterial peptide is as follows (SEQ ID NO: 2):
CTGGGTCTGGATTTCAAATTCAAAGAAAAGAAGAGCATTGGTTTTAAAGAGAAGCATATCCAAGTCCGCATCTTCAAGGACCTGAACCTGCGCGTTAAAACCGAATGGCAC。
preferably, the feeding is stopped not less than 16 hours before the transportation of the oplegnathus punctatus;
further, the transported oplegnathus punctatus weighs not less than 90 g.
During transportation, the water temperature is kept at 19-25 ℃, and the dissolved oxygen of the water body is kept at 7mg/L or above.
According to the invention, the feed rich in glycine, lysine and methionine is fed to the oplegnathus maculatus before transportation, so that the stress of transportation to the oplegnathus maculatus is effectively reduced, the adaptation time of the factory-cultured oplegnathus maculatus to the natural ecological environment after transportation is shortened, and the normal ingestion of the oplegnathus maculatus is promoted, so that the growth can be recovered more quickly, and meanwhile, the yeast capable of expressing the antibacterial peptide is added into the feed, so that the non-specific immunity of the oplegnathus maculatus can be improved, and the stress resistance to the environment is enhanced.
Drawings
FIG. 1: photo images of the oplegnathus fasciatus cultured in land-based industrial circulating water;
FIG. 2: the activity of lysozyme and interferon in the blood serum of the oplegnathus punctatus and the phagocytic index diagram of macrophages; wherein (A) lysozyme (B) interferon (C) phagocytosis index;
FIG. 3: the liver antioxidant index map of the oplegnathus punctatus comprises (A) superoxide dismutase, (B) glutathione peroxidase and (C) catalase.
Detailed Description
The applicant adopts the method that fasting is carried out 18 hours before transportation in the process of transporting the oplegnathus fasciatus, and excrement residual feed in a temporary culture pond is removed every 6 hours during the fasting period; in the transportation process, vitamin C is added into the water body; wherein the vitamin C is added at a concentration of 10-100mg/L, and if the transport time is less than 12h, the transport density of Oplegnathus punctatus is not more than 100kg/m3A body of water; if the transportation time is 12-24 hr, the density of transported Oplegnathus punctatus is not more than80kg/m3A body of water; if the transportation time exceeds 24h, the transportation density of the oplegnathus fasciatus does not exceed 65kg/m3A body of water; the dissolved oxygen of the water body is maintained at 7mg/L or more. After the transportation, the porgy is fed with the feed added with the bile acid, and after the feed added with the bile acid is fed for one week, the porgy is fed with the feed without the bile acid.
By the above method, although the mortality rate due to transport is significantly reduced; however, the ingestion rate of the oplegnathus punctatus is obviously influenced due to the change of the culture environment (from factory circulating water to deep and far sea nets and/or fences), and the ingestion rate is only 0.5% in the first week of transportation; this results in the inhibition of the growth of the transported oplegnathus fasciatus at the initial stage of the culture.
Through research on the amino acid composition of the liver of the oplegnathus punctatus before and after transportation, the contents of glycine, lysine and methionine in the liver of the oplegnathus punctatus are found to be obviously reduced compared with the contents of glycine, lysine and methionine in the liver of the oplegnathus punctatus one week after transportation before and after transportation. The present invention has been made on the basis of this finding.
The present invention will be described in detail below with reference to examples and the accompanying drawings.
Example 1: pagrus punctatus in land-based industrial culture stage
1) Classification screening of oplegnathus punctatus in land-based industrial breeding stage
The volume of the water pool for cultivation is 40m3The depth of the pond is 1.5m, and the depth of water injection is about 1 m. The culture water is seawater filtered by three-level sand, the incubation water temperature is about 22 ℃, the culture water temperature of the oplegnathus fasciatus fry is 24-26 ℃, the dissolved oxygen is more than 6mg/L, the oplegnathus fasciatus fry is continuously aerated slightly, the salinity is 24-28, the pH value is 7.9-8.4, the illumination intensity is 500-800 lx, and a dimming facility is configured. And after 20 days of age, carrying out dirt suction and bottom cleaning for 1 time every 4-5 days, continuously flowing water or discontinuously flowing water, wherein the daily flow is 12-18 times of the pond water, and selecting different culture densities (shown in table 1) according to the full length and the weight of the oplegnathus punctatus to obtain the optimal culture densities in different growth stages.
Table 1: survival rate and bait coefficient of oplegnathus fasciatus with different specifications under different culture densities
When the weight of the cultured oplegnathus fasciatus exceeds 90 grams, the culture density is kept at 60 tails/m3And preparing to transfer to a net cage and a fence for relay culture.
Example 2: transporting the Lutraria punctata cultured on land according to the existing method
1) Stopping feeding the oplegnathus fasciatus for not less than 16 hours before transportation, and preparing for transportation;
further, fasting is selected to be carried out 18 hours before transportation, and feces residue in the temporary rearing pond is removed every 6 hours during the fasting period;
2) in the transportation process, vitamin C is added into the water body; wherein the addition concentration of vitamin C is 10-100 mg/L;
wherein if the transportation time is less than 12h, the transportation density of the oplegnathus fasciatus is not more than 100kg/m3A body of water;
if the transportation time is 12-24 hr, the transported density of Oplegnathus punctatus is not more than 80kg/m3A body of water;
if the transportation time exceeds 24h, the transportation density of the oplegnathus fasciatus does not exceed 65kg/m3A body of water;
the dissolved oxygen of the water body is maintained at 7mg/L or more.
3) Feeding the porgy with the feed added with the bile acid after transportation, and feeding the porgy with the feed without the bile acid after one week;
wherein the feed added with bile acid is fed, and the feeding amount of the feed is 6 g/kg of body weight; the addition amount of bile acid is 2.0g/kg feed.
Through the measures, the oplegnathus maculatus is transported to a fence for culture, the transportation survival rate is 100%, the mortality rate is only 0.01% within 1 week after transportation, but the ingestion rate of the oplegnathus maculatus is not positive within the first week after transportation, the ingestion rate is only 0.5%, the ingestion of the oplegnathus maculatus is gradually recovered to be normal along with the prolonging of the culture period, the ingestion rate is 2.8% and the normal ingestion is gradually recovered within the 3 rd week after transportation. Therefore, although the transportation survival rate is guaranteed to be 100% by adopting the measures before, during and after the transportation, the ingestion movement of the oplegnathus fasciatus gradually returns to normal after 3 weeks of transportation, and the growth of the oplegnathus fasciatus is influenced in the early cultivation stage after the transportation and is almost in a stagnation state.
Example 3: analysis of amino acids in liver of oplegnathus fasciatus before and after transportation
The amino acid content in the liver was determined for the oplegnathus fasciatus cultured in the same culture pond of example 1 before and after the transport in the method of example 2 and one week of culture. Wherein 10 fish were selected for detection from non-transported and transported Oplegnathus fasciatus.
Freeze-drying liver tissue of the fish to be detected, and crushing the liver tissue by using a tissue homogenizer; the content of amino acid in liver is analyzed and determined by using an automatic amino acid analyzer. The measurement results are expressed as mean ± standard deviation, and the measurement data is analyzed by SPSS11.0 software.
The results showed that the contents of glycine ALA, lysine LYS and methionine MET in the liver were significantly reduced compared to the oplegnathus fasciatus one week after the transportation before the transportation (table 2); it is presumed that glycine ALA, lysine LYS and methionine MET are excessively consumed due to disturbance of metabolism of Oplegnathus punctatus induced by the transport process and stress reaction generated after transport to adapt to the natural environment.
Table 2: composition and content of partial amino acids in liver of oplegnathus fasciatus before and after transportation*Indicating significant difference)
Example 4: feeding feed rich in glycine, lysine and methionine and transferring
1. Feeding of bait
Feeding the feed rich in glycine, lysine and methionine 2 weeks before the transfer, wherein the glycine, lysine and methionine are added to the granular feed for oplegnathus punctatus by spray drying, and the addition amount of the glycine, lysine and methionine is 0.1-0.5% of the weight of the feed respectively.
Meanwhile, in order to improve the resistance of the oplegnathus punctatus and the nonspecific immunity of the oplegnathus punctatus, saccharomycetes capable of expressing the antibacterial peptide are added into the feed.
The antibacterial peptide is obtained by screening from an inhibitory subtractive hybrid library, and is prepared by injecting bacteria into the abdominal cavity of the oplegnathus punctatus for immunization, extracting liver tissues of an experimental group and a control group respectively, and obtaining a library of differentially expressed genes after inhibitory subtractive hybridization.
Considering that natural antimicrobial peptides are generally polypeptides consisting of less than 50 amino acid residues, the molecular weight is about 2000-5000 daltons. Therefore, a cDNA fragment having a fragment size of 200bp or less is selected from a cDNA library obtained after inhibitory subtractive hybridization of oplegnathus punctatus, the amino acid sequence of the encoded polypeptide is inputted into an Antimicrobial Peptide Database (APD) for correlation analysis, and the nucleotide fragment having a similarity higher than 40% is subjected to further antimicrobial analysis. The final screening yielded the antimicrobial peptide whose amino acid sequence is as follows (SEQ ID NO: 1):
LGLDFKFKEKKSIGFKEKHIQVRIFKDLNLRVKTEWH；
the sequence of the encoding gene is as follows (SEQ ID NO: 2):
CTGGGTCTGGATTTCAAATTCAAAGAAAAGAAGAGCATTGGTTTTAAAGAGAAGCATATCCAAGTCCGCATCTTCAAGGACCTGAACCTGCGCGTTAAAACCGAATGGCAC。
the antibacterial property of the antibacterial peptide is detected by a trace broth dilution method, the Minimum Inhibitory Concentration (MIC) is carried out on commonly used fish pathogenic bacteria, and detected strains comprise vibrio anguillarum, edwardsiella tarda, aeromonas salmonicida and escherichia coli. The Minimum Inhibitory Concentration (MIC) results for the strains are shown in table 3.
Table 3: minimum inhibitory concentration table for pathogenic bacteria
As can be seen from the above results, the antimicrobial peptides used in the present invention have excellent antimicrobial effects.
2. Transport sparus punctatus
The feeding is stopped 2 days before the transportation, and the weight of the transported Oplegnathus punctatus is not less than 90 g. During transportation, the water temperature is kept at 19-25 ℃, and the dissolved oxygen of the water body is maintained above 7 mg/L. Wherein if the transportation time is less than 12h, the transportation density of the oplegnathus fasciatus is not more than 100kg/m3A body of water; if the transportation time is 12-24 hr, the transported density of Oplegnathus punctatus is not more than 80kg/m3A body of water; if the transportation time exceeds 24h, the transportation density of the oplegnathus fasciatus does not exceed 65kg/m3A body of water.
Example 5: detection of economic traits such as growth and resistance after transportation
Selecting 60,000 oxpoch 90g in 60,000 XUE in factory circulating water workshop in late 6 Yue of 2020, adding yeast rich in glycine, lysine and methionine and recombinantly expressing antibacterial peptide into oxpoch feed by the method of example 4, starting transportation after two weeks, fasting for 18 hours before transportation, selecting clear weather, and passing through live fish truck (transportation density 100 kg/m)3Water body, transportation time is 2 hours) to the wharf, and then the fish is transported by a professional live fish transport ship (transportation density is 100 kg/m)3Water, transport time 4 hours) to open sea breeding pens.
The swimming ingestion condition of the oplegnathus fasciatus is observed, the swimming and ingestion state of the oplegnathus fasciatus is obviously improved after 1 week, the ingestion rate reaches 1.0 percent within 1 week, the statistical transportation survival rate is 100 percent, the mortality rate is only 0.016 percent (death rate is 10 tails) within 1 week after transportation, the body weight is increased to 235 grams after 3 months of culture, and the mortality rate is 0.06 percent (death rate is 40 tails).
And the oplegnathus punctatus with the same specification and size is normally fed without yeast, glycine, lysine and methionine, is not fasted and is not fed in a limited manner by adopting a conventional transportation method, and the result shows that the transportation survival rate is 99.45 percent (death 332 tails) and the mortality rate in one week is 1.12 percent (death 672 tails), and the weight of the oplegnathus punctatus is increased to 198 g and the mortality rate is 0.35 percent (death 212 tails) after 3 months of culture. 1500 sparus punctatus of the same specification and size is cultured in an industrial circulating water workshop all the time, after 3 months of culture, the weight is increased to 179 g, and the death rate is 1.06% (16 tails died).
Meanwhile, the non-specific immune indexes (lysozyme, interferon and phagocytic index) and liver oxidation resistance indexes (superoxide dismutase, glutathione peroxidase and catalase) of the oplegnathus fasciatus are detected, and the results show that the activities of serum lysozyme, interferon and macrophage phagocytic index of the oplegnathus fasciatus are obviously higher than those of an unadditized group and an industrial culture circulating water culture group (figure 2) by adding saccharomycetes, glycine, lysine and methionine into the feed, and the activities of liver superoxide dismutase, glutathione peroxidase and catalase also show similar change trends. This indicates that by adopting the feeding strategy of the invention, the nonspecific immunity of the oplegnathus fasciatus is remarkably improved (figure 3), and the disease resistance is remarkably enhanced.
Claims (5)
1. A method for relay culture of oplegnathus punctatus on land and sea is characterized in that the oplegnathus punctatus cultured on land is transported to a net cage and/or a fence for culture at the beginning of 6 months at the bottom of 5 months; feeding a feed rich in glycine, lysine and methionine before transport; and yeast capable of expressing the antibacterial peptide is added into the feed; the amino acid sequence of the antibacterial peptide is SEQ ID NO. 1.
2. The method of claim 1 wherein the feed is fed glycine, lysine and methionine rich feed 10-15 days prior to transport.
3. The method of claim 1, wherein the feeding is stopped not less than 16 hours prior to the transport of the oplegnathus fasciatus.
4. The method of claim 1, wherein the weight of the transported oplegnathus punctatus is not less than 90 g.
5. The method of claim 1, wherein the water temperature is maintained at 19-25 ℃ and the dissolved oxygen in the water is maintained at 7mg/L or more during transportation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110792861.8A CN113519417B (en) | 2021-07-14 | 2021-07-14 | Luhai relay breeding method for oplegnathus punctatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110792861.8A CN113519417B (en) | 2021-07-14 | 2021-07-14 | Luhai relay breeding method for oplegnathus punctatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113519417A CN113519417A (en) | 2021-10-22 |
| CN113519417B true CN113519417B (en) | 2022-03-29 |
Family
ID=78127831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110792861.8A Active CN113519417B (en) | 2021-07-14 | 2021-07-14 | Luhai relay breeding method for oplegnathus punctatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113519417B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641025A (en) * | 2004-11-29 | 2005-07-20 | 中国水产科学研究院黄海水产研究所 | Pagrosomus major antibacterial peptide gene, and recombinant yeast expression vector and its preparing method |
| CN101063145A (en) * | 2007-04-20 | 2007-10-31 | 厦门大学 | Expression carrier for black porgy antibiotic peptide Hepcidin and expression product and constructing preparation method |
| JP2014147378A (en) * | 2013-02-04 | 2014-08-21 | Fisheries Research Agency | Method for breeding fish having high feed efficiency |
| CN110402856A (en) * | 2019-08-30 | 2019-11-05 | 三亚福联水产发展有限公司 | High-yield culture method for groupers |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101164431A (en) * | 2007-10-08 | 2008-04-23 | 国家海洋局第一海洋研究所 | Feed additive with antibiotic function and application |
| CN102406091B (en) * | 2011-12-02 | 2013-03-20 | 中国水产科学研究院淡水渔业研究中心 | Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof |
| GB201204816D0 (en) * | 2012-03-19 | 2012-05-02 | Brainco Biopharma S L | Transgenic animal model of mood disorders |
| CN104396841B (en) * | 2014-12-20 | 2015-07-29 | 中国水产科学研究院黄海水产研究所 | Pearl rough gentian grouper and rock porgy industrial circulating water mixed cultivation method |
| CN106719126A (en) * | 2016-11-25 | 2017-05-31 | 防城港市畜牧站 | A kind of method of cage culture crucian |
| CN107173268A (en) * | 2017-06-06 | 2017-09-19 | 舟山市普陀兴海养殖优质种苗选育研究所 | A kind of artificial seed breeding method of Epinephelus coioides |
| CN107480884B (en) * | 2017-08-14 | 2019-10-22 | 水利部交通运输部国家能源局南京水利科学研究院 | A kind of more gate dam plain tract wintering ecology flow calculation methods |
| CN109874706A (en) * | 2019-03-28 | 2019-06-14 | 三亚市海洋与渔业监测中心 | A kind of trans-regional adaptation cultural method of rock porgy fish |
| CN110622892B (en) * | 2019-10-31 | 2022-02-11 | 宁德市富发水产有限公司 | Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method |
| CN111149761B (en) * | 2020-02-26 | 2022-01-07 | 中国水产科学研究院黄海水产研究所 | Method for reducing stress in transportation process of oplegnathus punctatus |
-
2021
- 2021-07-14 CN CN202110792861.8A patent/CN113519417B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641025A (en) * | 2004-11-29 | 2005-07-20 | 中国水产科学研究院黄海水产研究所 | Pagrosomus major antibacterial peptide gene, and recombinant yeast expression vector and its preparing method |
| CN101063145A (en) * | 2007-04-20 | 2007-10-31 | 厦门大学 | Expression carrier for black porgy antibiotic peptide Hepcidin and expression product and constructing preparation method |
| JP2014147378A (en) * | 2013-02-04 | 2014-08-21 | Fisheries Research Agency | Method for breeding fish having high feed efficiency |
| CN110402856A (en) * | 2019-08-30 | 2019-11-05 | 三亚福联水产发展有限公司 | High-yield culture method for groupers |
Non-Patent Citations (2)
| Title |
|---|
| "大菱鲆抗菌肽基因酵母表达载体的构建及表达";李伟等;《长江大学学报》;20081231;第5卷(第4期);第61-66页 * |
| 大黄鱼抗菌肽hepcidin在巴斯德毕赤酵母中的表达及其产物的抑菌活性;汪玉华等;《应用海洋学学报》;20130815;第32卷(第3期);第383-389页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113519417A (en) | 2021-10-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Benetti et al. | Aquaculture of cobia (Rachycentron canadum) in the Americas and the Caribbean | |
| CN111149761B (en) | Method for reducing stress in transportation process of oplegnathus punctatus | |
| Fletcher et al. | Preliminary observations on the reproductive biology of ornamental cleaner prawns Stenopus hispidus Lysmata amboinensis Lysmata debelius | |
| CN102461470A (en) | Low-salinity artificial seedling raising and fresh water aquiculture method for takifugu flavidus | |
| KR20140115445A (en) | METHOD FOR CULTUERING CATADROMOUS FISH(Anguilla marmorata) BY USING RECIRCULATING AQUACULTUER SYSTEM AND SEAWATER ACCLIMATION | |
| CN104186393A (en) | Jellyfish breeding method | |
| CN114766397B (en) | Cultivation method of bellied sea horse | |
| CN104161001B (en) | A kind of Tilapia mossambica and loach pond polyculture method | |
| CN113519417B (en) | Luhai relay breeding method for oplegnathus punctatus | |
| CN1151714C (en) | Low-salinity artificial brooding and fresh-water culturing method for fugus | |
| CN113197135B (en) | Long-distance transportation mode for parent litopenaeus vannamei | |
| EP3526316B1 (en) | Process for producing methanol and/or methane | |
| CN101971783A (en) | Full artificial ecological simulation breeding technology of burbot | |
| CN112425535A (en) | Method for accelerating early fry feeding habit conversion of takifugu rubripes | |
| Qiu et al. | Effect of culture methods on individual variation in the growth of sea cucumber Apostichopus japonicus within a cohort and family | |
| CN113892450A (en) | Method for culturing small yellow croakers in deep water net cages | |
| CN103988798A (en) | Method for performing wintering breeding on large yellow croaker | |
| CN106973838B (en) | Limited feeding method for cage culture of trachinotus ovatus | |
| WO2019200103A1 (en) | A feed ingredient comprising clostridium tyrobutyricum | |
| CN110476845A (en) | A kind of factory culturing method of Bambusa fish fingerling | |
| Tholasilingam et al. | Prospects on spiny lobster Panulirus spp. culture in the east coast of India | |
| Pyanov | A review of the cultivation potential of turbot (Scophthalmus maximus (L.)) in the Baltic Sea region: a promising candidate species for marine aquaculture in Russia | |
| CN114073232B (en) | Breeding method and application of bred parent trachidermus fasciatus | |
| CN111213601B (en) | Indoor cement pond artificial breeding method for intercross hybrid of humpback bass and epinephelus lanceolatus | |
| Al-Maliky | Breeding of Shrimps Metapenaeus affinis (H. Milne Edwards, 1837) in Cages |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |