CN113509409A - Preparation method and application of galangal seed flower essential oil - Google Patents

Preparation method and application of galangal seed flower essential oil Download PDF

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CN113509409A
CN113509409A CN202110842841.7A CN202110842841A CN113509409A CN 113509409 A CN113509409 A CN 113509409A CN 202110842841 A CN202110842841 A CN 202110842841A CN 113509409 A CN113509409 A CN 113509409A
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essential oil
galangal
flower
flower essential
cosmetic
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CN113509409B (en
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李仁�
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Xishuangbanna Tropical Botanical Garden of CAS
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Xishuangbanna Tropical Botanical Garden of CAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Epidemiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses a preparation method and application of galangal seed flower essential oil, and belongs to the field of cosmetics. Taking the galangal flower, adding a proper amount of ultrapure water, distilling and extracting for 4 hours by using steam, collecting distillate, separating liquid, and drying by using anhydrous sodium sulfate to obtain the galangal flower essential oil. The galangal seed flower essential oil is prepared into cosmetics including emulsions, creams, masks, sprays, essences, essential oils and soaps according to the addition amount of 0.01-100%. The research of the invention finds that the natural plant essential oil distilled and extracted from the red cardamom flower has the activity of obviously inhibiting staphylococcus aureus, candida albicans, trichophyton rubrum, epidermophyton floccosum and microsporum gypseum, and the activity of tyrosinase is obviously inhibited, and particularly shows the antioxidant activity superior to that of the rose essential oil in DPPH, ABTS and FRAP experiments. The galangal flower essential oil is used for preparing cosmetics such as emulsions, creams, masks, sprays, essences, essential oils and soaps, and has remarkable antibacterial, whitening and antioxidant effects.

Description

Preparation method and application of galangal seed flower essential oil
Technical Field
The invention discloses a preparation method and application of galangal fruit flower essential oil, and particularly relates to galangal fruit flower essential oil with antibacterial, whitening and antioxidant activities and application of the galangal fruit flower essential oil, belonging to the field of cosmetics.
Background
Alpinia galanga (L.) Willd.) is a plant of the genus Alpinia of the family Zingiberaceae and is widely distributed in tropical regions of Asia. In provinces such as Taiwan, Guangdong, Guangxi and Yunnan provinces, the plants grow under mountain valley shade wet forests with the elevation of 100 + 1300 m or in shrubs and grass clusters. The fructus Galangae is called fructus Galangae for medical use, and has the functions of removing dampness, dispelling cold, activating spleen and promoting digestion. The rhizome, rhizoma Alpiniae Galangae, is also used for cold pain in stomach and vomiting and diarrhea due to spleen cold, because it is pungent in flavor and hot in nature, and can dispel cold, warm stomach and alleviate pain. The current research on the galangal seeds mainly focuses on the chemical component analysis and related pharmacological activity research of the galangal seeds and root tubers, and no report is found on the research on the galangal flower essential oil.
Essential oil is also called volatile oil and aromatic oil, and is a volatile and fat-soluble oily substance contained in aromatic plants. The essential oil has complicated chemical components, and can be divided into terpenoids, aromatic compounds, aliphatic compounds, sulfur-containing nitrogen-containing heterocyclic compounds and special compounds with macrocyclic lactone structures according to the structure. The essential oil has various physiological activities, including antibacterial, antiinflammatory, antivirus, analgesic, antitumor, mosquito repelling, penetration promoting, antioxidant, etc. Therefore, in order to find a new use of the galangal oil, the present invention has carried out related studies. The prior art does not find the application of the galangal seed essential oil in the field of preparing cosmetics with the functions of bacteriostasis, whitening and antioxidation.
Based on the analysis, the invention provides the cosmetic added with the galangal seed essential oil, so that the cosmetic has the effects of bacteriostasis, whitening and antioxidation.
Disclosure of Invention
Aiming at the technical problems, the invention provides the galangal seed flower essential oil with bacteriostatic, whitening and antioxidant activities and the application thereof.
The purpose of the invention is realized by the following technical scheme:
an application of flos Galangae essential oil comprises the application of flos Galangae essential oil in preparing cosmetics.
Further, the cosmetic has antibacterial, whitening and antioxidant effects.
Further, the cosmetic includes, but is not limited to: emulsions, creams, masks, sprays, essences, essential oils, soaps.
Furthermore, the galangal flower essential oil is obtained by a steam distillation method.
Furthermore, the addition amount of the galangal seed flower essential oil in the cosmetics is 0.01% -100%.
The invention also discloses a cosmetic which contains the galangal flower essential oil.
Furthermore, the cosmetic contains 0.01-100% of galangal seed flower essential oil.
Further, the cosmetic has antibacterial, whitening and antioxidant effects.
Further, the cosmetics include, but are not limited to: emulsions, creams, masks, sprays, essences, essential oils, soaps.
The invention has the beneficial effects that:
the invention provides the application of the galangal flower essential oil in preparing the cosmetics with the effects of bacteriostasis, whitening and antioxidation, lays a material foundation for further exploring the functions and biological activities of the galangal flower essential oil, provides scientific basis for reasonably developing and utilizing the plant resource, and finally provides a new resource for promoting the industrial development of the cosmetics with the effects of bacteriostasis, whitening and antioxidation.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
Preparation of galangal seed flower essential oil by steam distillation
Adding 3 times of ultrapure water into fresh flos Galangae, extracting for 4 hr by steam distillation, collecting distillate, and drying with anhydrous sodium sulfate to obtain flos Galangae essential oil with yield of 0.05-0.1%. The galangal seed essential oil is bottled with brown essential oil and stored in a refrigerator at 4 ℃ for later use.
Application example 1
Preparation of an oil emulsion of Alpinia galanga flower
The cleaning agent comprises, by mass, tween 602%, glycerol 3%, plukenetia volubilis oil 10%, disodium EDTA 0.01%, shea butter 2%, butanediol 6.00%, fructus Galangae flower essential oil 0.5%, and the balance ultrapure water. The preparation method of the emulsion is a conventional process in the industry, and the galangal flower essential oil prepared in example 1 is selected in the application example.
Application example 2
Preparation of galangal fruit flower essential oil mask
The anti-rust cleaning agent comprises, by mass, 3% of glycerin, 1% of polyethylene glycol, 1% of propylene glycol, 2% of plukenetia volubilis oil, 15.00% of carbomer 9411% aqueous solution, 0.10% of sodium hyaluronate, 0.01% of disodium EDTA, 1.00% of panthenol, 0.5% of galangal flower essential oil and the balance of ultrapure water. The preparation method of the mask is a conventional process in the industry, and the galangal seed flower essential oil prepared in example 1 is selected in the application example.
Application example 3
Preparation of galangal seed flower essential oil spray
The composite material comprises, by mass, 5% of isobutane, 75% of vodka, 5% of ethylhexyl methoxycinnamate, 2% of glycerol, 0.2% of sodium hyaluronate, 5% of galangal flower essential oil and the balance of ultrapure water. The essential oil spray preparation method is a conventional process in the industry, and the essential oil of the galangal seeds flower prepared in example 1 is selected in the application example.
Application example 4
Preparation of galangal seed flower essential oil soap
100g of coconut oil, 50g of glycerin, 100g of plukenetia volubilis oil, 50g of shea butter, 50g of sodium hydroxide, 150ml of ultrapure water and 1g of galangal flower essential oil. The preparation method of the essential oil soap is a conventional process in the industry, and the galangal seed flower essential oil prepared in example 1 is selected in the application example.
Test example 1
Test for bacteriostatic Activity
Staphylococcus aureus aureobasip (aureus subsp. aureous) was purchased from the china general microbiological culture collection center; candida albicans (Candida albicans) ATCC10231 was purchased from Microbiolics, USA; epidermophyton floccosum (Epidermophyton floccosum) CBS 566.94, Trichophyton rubrum (Trichophyton rubrum) ATCC4438, Microsporum gypseum (Microsporum gypseum) CBS118893 were purchased from the medical fungi collection center of Chinese academy of medical sciences.
(1) Antibacterial experiments
Taking a culture plate with 96 holes, and culturing,diluting the sample to be tested, adding bacterial liquid into each hole, and obtaining the final concentration of 5 multiplied by 105CFU/mL; culturing at 37 deg.C for 24 hr, and detecting light absorption value at 625nm with microplate reader. The experiment was also set with a medium blank control, a bacteria control, and a ceftazidime, penicillin G sodium positive drug control.
(2) Antifungal experiments
Diluting the sample to be tested with 96-well culture plate, adding fungus bacterial liquid into each well to obtain final concentration of 1 × 105CFU/mL (Candida albicans) and 5X 105CFU/mL (filamentous fungus), Candida albicans cultured at 37 ℃ for 24 hours, filamentous fungus cultured at 25 ℃ for 5 days, and enzyme-linked immunosorbent assay (ELISA) for detecting the light absorption value at 625 nm. The experiment is simultaneously provided with a culture medium blank control, a fungus control, an amphotericin B and terbinafine hydrochloride positive drug control. Minimum bactericidal concentration MIC90(90% minimum inhibition concentration) by Reed&And (4) calculating by a Muench method.
Specific results are shown in table 1:
TABLE 1 antibacterial Activity of Alpinia galanga flower essential oil
Bacterial strain MIC90(μg/mL)
Staphylococcus aureus 230.501±1.053
Candida albicans 485.184±5.258
Trichophyton rubrum 188.525±2.006
Epidermophyton floccosum 12.36±0.257
Microsporidian gypseum 87.568±3.237
According to the test results in table 1, it is concluded that the essential oil of alpinia galanga has good bacteriostatic activity by significantly inhibiting the effects of staphylococcus aureus, candida albicans, trichophyton rubrum, epidermophyton floccosum and microsporum gypseum.
Test example 2
Assay for inhibition of tyrosinase Activity
Mixing samples to be tested with different concentrations and L-Dopa (final concentration is 1.25mM), adding tyrosinase (final concentration is 25U/mL) to start reaction, setting 3 repeated holes, setting a blank control without drugs and a Kojic Acid positive control, reacting for 5 minutes at room temperature, and measuring the OD value by using an enzyme labeling instrument at the wavelength of 490 nm. And calculating to obtain the tyrosinase activity inhibition rate.
Tyrosinase activity inhibition (%) (1-sample well OD)490nmExperiment blank control well OD490nm)×100
The specific results are shown in table 2:
TABLE 2 inhibition of tyrosinase activity by Alpinia galanga flower essential oil
Sample (I) IC50(μg/mL)
Kojic Acid Kojic Acid 4.835±0.258
Fructus galangae flower essenceOil 31.159±0.481
According to the test results in table 2, the galangal flower essential oil significantly inhibits tyrosinase activity, so that the galangal flower essential oil has a good whitening function.
Test example 3
Test for antioxidant Activity
(1) DPPH radical scavenging test
Adding 100 μ L of sample extractive solutions with different concentrations into 96-well plate, adding 100 μ L of DPPH solution (100umoL/L), reacting in dark for 30 min, measuring light absorption value at 517, detecting antioxidant activity of the extract with anhydrous ethanol as blank control and VC as positive control, repeating the steps for 3 concentrations, and taking the average value. DPPH free radical clearance calculation formula:
DPPH free radical clearance rate (D0-D1)/A0%
In the formula, D1 is the absorbance of the plant essential oil after the reaction with DPPH solution;
d0 is the absorbance of the reaction mixture of absolute ethanol and DPPH solution.
(2) ABTS free radical scavenging assay
The ABTS. + reagent is prepared by dissolving 0.0384g ABTS and 0.0060g ammonium persulfate in 10mL of ultrapure water and reacting for 12-16h in a dark place, the solution needs to be prepared 1 day ahead of time and must be used on the same day. Before use, the extract is diluted with anhydrous ethanol until the absorbance value is 0.70 +/-0.02 at 732 nm. A96-well plate was used, and 10. mu.L of samples of different concentrations were added to 190. mu.L of ABTS+In the solution, after reacting for 6min at room temperature in a dark place, measuring the light absorption value by an enzyme-labeling instrument under the condition of 732nm, repeating the experiment for 3 times for each sample, and taking the average value. The ABTS free radical clearance calculation formula is:
ABTS free radical clearance rate (A0-A1)/A0 is 100%
In the formula, A1 is the absorbance of the reaction between plant essential oil and ABTS solution;
a0 is the absorbance of absolute ethanol after reaction with ABTS solution.
(3) FRAP iron ion reduction capability test
Preparation of TPTZ solution: 25mL of 0.3mol/L acetate buffer solution, 2.5mL of 10mmol/L TPTZ working solution and 20mmol/L FeCl3The solution is composed of 2.5mL and is prepared as before. Taking a 96-well plate, respectively adding 10 mu L of samples with different concentrations and 180 mu L of TPTZ solution, uniformly mixing, reacting for 10min at 37 ℃, measuring the light absorption value at 593nm by an enzyme-labeling instrument, repeating the experiment for 3 times for each sample, and taking the average value. Trolox is used as a standard substance, and the iron ion reduction Capacity of the essential oil sample is represented by Trolox-Equivalent Antioxidant Capacity TEAC (Trolox-Equivalent Antioxidant Capacity).
Specific results are shown in table 3:
TABLE 3 anti-oxidant Activity of Alpinia galanga flower essential oil
Sample (I) DPPH IC50(mg/ml) ABTS IC50(mg/ml) FRAP TEAC(mM)
Fructus Galangae flower essential oil 0.25 1.41 0.73
Rose essential oil 0.53 4.34 0.19
Note: radical median scavenging concentration IC50The smaller the value, the stronger the antioxidant activity; FRAP TEAC is the same antioxidant ability value of Trolox when the concentration of the galangal flower essential oil and the rose flower essential oil is 2.5mg/ml, and the larger the TEAC value is, the stronger the antioxidant activity is.
According to the test results in table 3, the galangal flower essential oil shows superior antioxidant activity to rose essential oil in DPPH, ABTS and FRAP antioxidant tests, and shows good application prospect in antioxidant products.
In a word, the test result shows that the galangal flower essential oil has obvious antibacterial, whitening and antioxidant effects when being applied to preparation of cosmetics such as emulsions, creams, masks, sprays, essences, essential oils, soaps and the like.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (9)

1. Application of galangal fruit flower essential oil comprises application of galangal fruit flower essential oil in preparation of cosmetics.
2. The use of galangal flower essential oil according to claim 1, wherein the cosmetic has bacteriostatic, whitening and antioxidant effects.
3. Use of an essential oil of alpinia galanga flowers according to claim 1 or 2, wherein the cosmetic comprises but is not limited to: emulsions, creams, masks, sprays, essences, essential oils, soaps.
4. The use of the essential oil of Alpinia galanga flowers according to any one of claims 1 to 3, wherein the essential oil of Alpinia galanga flowers is prepared by steam distillation.
5. The application of the essential oil of galangal flowers according to any one of claims 1 to 3, wherein the essential oil of galangal flowers is added in an amount of 0.01% to 100% in a cosmetic.
6. A cosmetic comprising the galangal flower essential oil according to claim 4.
7. The cosmetic according to claim 6, wherein the cosmetic contains 0.01% to 100% of galangal flower essential oil.
8. The cosmetic according to claim 6 or 7, wherein the cosmetic has bacteriostatic, whitening and antioxidant effects.
9. The cosmetic according to claim 6 or 7, wherein the cosmetic comprises but is not limited to: emulsions, creams, masks, sprays, essences, essential oils, soaps.
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CN114869847A (en) * 2022-03-31 2022-08-09 中国热带农业科学院分析测试中心 Amomum katsumadai essential oil microemulsion and preparation method thereof

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Application publication date: 20211019

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