CN113484248A - High-sensitivity glucose concentration detection device and method based on four-mirror resonant cavity - Google Patents
High-sensitivity glucose concentration detection device and method based on four-mirror resonant cavity Download PDFInfo
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 73
- 239000008103 glucose Substances 0.000 title claims abstract description 73
- 238000001514 detection method Methods 0.000 title claims abstract description 42
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- 239000012153 distilled water Substances 0.000 claims description 8
- 238000002310 reflectometry Methods 0.000 claims description 8
- 238000002834 transmittance Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 230000008033 biological extinction Effects 0.000 claims description 3
- WUKWITHWXAAZEY-UHFFFAOYSA-L calcium difluoride Chemical compound [F-].[F-].[Ca+2] WUKWITHWXAAZEY-UHFFFAOYSA-L 0.000 claims description 3
- 229910001634 calcium fluoride Inorganic materials 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims 1
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- 238000004186 food analysis Methods 0.000 description 1
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Abstract
The invention relates to a glucose concentration detection technology, in particular to a high-sensitivity glucose concentration detection device and method based on a four-mirror resonant cavity. The invention solves the problem that the existing optically-active glucose concentration detection device can not have both high sensitivity and small volume. A high-sensitivity glucose concentration detection device based on a four-mirror resonant cavity comprises a light source, a polarizer, the four-mirror resonant cavity, an analyzer, a detector and an optical rotation tube; wherein, the emergent end of the light source is opposite to the incident end of the polarizer; the exit end of the polarizer is obliquely opposite to the incident end of the four-mirror resonant cavity; the emergent end of the four-mirror resonant cavity is obliquely opposite to the incident end of the analyzer; the emergent end of the analyzer is opposite to the incident end of the detector; the optical rotation tube is arranged on a resonant light path of the four-mirror resonant cavity. The invention is not only suitable for detecting the concentration of glucose, but also suitable for detecting the concentrations of other optical rotation substances.
Description
Technical Field
The invention relates to a glucose concentration detection technology, in particular to a high-sensitivity glucose concentration detection device and method based on a four-mirror resonant cavity.
Background
Glucose concentration detection is an important component of food analysis technology and also a basic means for human health monitoring. Among the various glucose concentration detection devices, the most common one is an optically active glucose concentration detection device which is produced by utilizing the optical activity of glucose. The optical rotation type glucose concentration detection device generally comprises a light source, a polarizer, an optical rotation tube, an analyzer and a detector, and the principle is that the glucose concentration is detected according to the linear relation between the optical rotation angle and the glucose concentration (the ratio of the optical rotation angle to the glucose concentration is the sensitivity). Under the condition of the prior art, the optically active glucose concentration detection device has the problem that the optically active glucose concentration detection device cannot have high sensitivity and small volume due to the limit of the structure of the optically active glucose concentration detection device. Specifically, in the conventional optically active glucose concentration detection device, in order to improve the sensitivity of the device, it is necessary to increase the length of the optically active tube. And once the length of the optical rotation tube is increased, the volume of the device is inevitably overlarge. Therefore, a high-sensitivity glucose concentration detection device and a high-sensitivity glucose concentration detection method based on a four-mirror resonant cavity are needed to be invented to solve the problem that the conventional optical rotation type glucose concentration detection device cannot have both high sensitivity and small volume.
Disclosure of Invention
The invention provides a high-sensitivity glucose concentration detection device and method based on a four-mirror resonant cavity, aiming at solving the problem that the existing optical rotation type glucose concentration detection device cannot have high sensitivity and small volume.
The invention is realized by adopting the following technical scheme:
a high-sensitivity glucose concentration detection device based on a four-mirror resonant cavity comprises a light source, a polarizer, the four-mirror resonant cavity, an analyzer, a detector and an optical rotation tube; wherein, the emergent end of the light source is opposite to the incident end of the polarizer; the exit end of the polarizer is obliquely opposite to the incident end of the four-mirror resonant cavity; the emergent end of the four-mirror resonant cavity is obliquely opposite to the incident end of the analyzer; the emergent end of the analyzer is opposite to the incident end of the detector; the optical rotation tube is arranged on a resonant light path of the four-mirror resonant cavity.
A high-sensitivity glucose concentration detection method based on a four-mirror resonant cavity (the method is realized based on the high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity), which is realized by adopting the following steps:
the method comprises the following steps: injecting distilled water into the optical rotation tube;
step two: turning on a light source, wherein a light beam emitted by the light source is changed into a linearly polarized light beam through a polarizer and then enters a four-mirror resonant cavity; the linear polarized light beam incident to the four-mirror resonant cavity is mostly reflected, and the small part of the linear polarized light beam enters the four-mirror resonant cavity; most of linearly polarized light beams entering the four-mirror resonant cavity are circularly transmitted for multiple times along a resonant light path of the four-mirror resonant cavity and are emitted after penetrating through the optical rotation tube for multiple times, and a small part of the linearly polarized light beams are emitted after directly penetrating through the four-mirror resonant cavity and the optical rotation tube; the emergent linearly polarized light beam part is transmitted through the analyzer and then enters the detector; the transmitted light intensity of the linearly polarized light beam can be observed through a detector;
step three: rotating the analyzer for a circle, and observing the change of the transmitted light intensity through the detector in the rotating process, thereby determining the minimum value of the transmitted light intensity; defining the angle of the analyzer when the transmitted light intensity reaches the minimum value as the initial angle theta0Then the light source is turned off;
step four: firstly, the analyzer is rotated to an initial angle theta0Then, emptying distilled water, injecting a glucose solution with standard concentration into the optical rotation tube, and then executing the second step; at this time, the transmitted light intensity observed by the detector is greater than the minimum value due to the optical rotation effect of glucose;
step five: rotating the analyzer, and observing the change of the transmitted light intensity through the detector in the rotating process until the transmitted light intensity reaches the minimum value again; defining the angle of the analyzer when the transmitted light intensity reaches the minimum value again as the first angle theta1Then the light source is turned off;
step six: according to a first angle theta1And a starting angle theta0Calculating a first rotation angle alpha1According to the first rotation angle alpha1Calculating the sensitivity k; the specific calculation formula is as follows:
α1＝θ1-θ0；
in the formula: c1Denotes the standard concentration, and C1Is a known amount;
step seven: firstly, the analyzer is rotated to an initial angle theta0Emptying the glucose solution with the standard concentration, injecting the glucose solution to be detected into the optical rotation tube, and then executing the second step; at this time, the transmitted light intensity observed by the detector is greater than the minimum value due to the optical rotation effect of glucose;
step eight: rotating the analyzer, and observing the change of the transmitted light intensity through the detector in the rotating process until the transmitted light intensity reaches the minimum value again; defining the angle of the analyzer when the transmitted light intensity reaches the minimum value again as a second angle theta2Then the light source is turned off;
step nine: according to a second angle theta2And a starting angle theta0Calculating a second rotation angle alpha2According to the second rotation angle alpha2And calculating the concentration C of the glucose solution to be detected according to the sensitivity k2(ii) a The specific calculation formula is as follows:
α2＝θ2-θ0；
compared with the existing optical rotation type glucose concentration detection device, the high-sensitivity glucose concentration detection device and method based on the four-mirror resonant cavity enable the light beam to penetrate through the optical rotation tube for multiple times by arranging the four-mirror resonant cavity, and therefore multiple accumulation of optical rotation effects is achieved. Based on multiple accumulation of optical rotation effect, the optical rotation angle is obviously amplified, so that the sensitivity is obviously improved on the premise of not increasing the length of the optical rotation tube, and the optical rotation tube has high sensitivity and small volume.
The optical rotation type glucose concentration detection device is reasonable in structure and ingenious in design, effectively solves the problem that the existing optical rotation type glucose concentration detection device cannot have high sensitivity and small volume at the same time, and is suitable for detecting the concentration of glucose and other optical rotation substances.
Drawings
Fig. 1 is a first structural schematic diagram of the present invention.
Fig. 2 is a second structural schematic of the present invention.
In the figure: 1-light source, 2-polarizer, 301-first cavity mirror, 302-second cavity mirror, 303-third cavity mirror, 304-fourth cavity mirror, 4-analyzer, 5-detector and 6-optical rotation tube.
Detailed Description
Example one
A high-sensitivity glucose concentration detection device based on a four-mirror resonant cavity comprises a light source 1, a polarizer 2, the four-mirror resonant cavity, an analyzer 4, a detector 5 and an optical rotation tube 6; wherein, the emergent end of the light source 1 is opposite to the incident end of the polarizer 2; the emergent end of the polarizer 2 is obliquely opposite to the incident end of the four-mirror resonant cavity; the emergent end of the four-mirror resonant cavity is obliquely opposite to the incident end of the analyzer 4; the emergent end of the analyzer 4 is opposite to the incident end of the detector 5; the optical rotation tube 6 is arranged on a resonant light path of the four-mirror resonant cavity.
In the present embodiment, as shown in FIG. 1, the four-mirror resonator comprises first to fourth mirrors 301-304 arranged in a rectangular shape; the first cavity mirror 301 and the second cavity mirror 302 are both semi-transparent and semi-reflective mirrors; the third cavity mirror 303 and the fourth cavity mirror 304 are both high-reflection mirrors; the front incident end of the first cavity mirror 301 is used as the incident end of the four-mirror resonant cavity; the back transmission end of the first cavity mirror 301 is diagonally opposite to the front incident end of the second cavity mirror 302; the back transmission end of the second cavity mirror 302 is used as the exit end of the four-mirror resonant cavity; the front reflection end of the second cavity mirror 302 is diagonally opposite to the incident end of the third cavity mirror 303; the reflection end of the third mirror 303 is diagonally opposite to the incident end of the fourth mirror 304; the reflection end of the fourth cavity mirror 304 is obliquely opposite to the incident end of the back surface of the first cavity mirror 301; the back reflection end of the first cavity mirror 301 is diagonally opposite to the front incident end of the second cavity mirror 302; the first to fourth cavity mirrors 301 to 304 form a rectangular resonant optical path together.
The transmittance of the first cavity mirror 301 is less than 10%; the transmittance of the second cavity mirror 302 is less than that of the first cavity mirror 301; the reflectivity of the third cavity mirror 303 and the reflectivity of the fourth cavity mirror 304 are both greater than 99.5%, and the focal lengths of the third cavity mirror and the fourth cavity mirror are the same.
The light source 1 adopts a laser with the output wavelength of 500 nm-760 nm; the polarizer 2 adopts a linear polaroid with an extinction ratio of more than 1: 1000; the analyzer 4 adopts an electric analyzer with a differential controller; the detector 5 comprises a photosensitive element, a data acquisition card and a computer; the incident end of the photosensitive element is used as the incident end of the detector 5; the output end of the photosensitive element is connected with the input end of the data acquisition card; the output end of the data acquisition card is connected with the input end of the computer; the optical rotation tube 6 is a quartz tube or an optical rotation tube with a calcium fluoride window.
A high-sensitivity glucose concentration detection method based on a four-mirror resonant cavity (the method is realized based on the high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity), which is realized by adopting the following steps:
the method comprises the following steps: distilled water is injected into the optical rotation tube 6;
step two: the light source 1 is turned on, and light beams emitted by the light source 1 are changed into linearly polarized light beams through the polarizer 2 and then enter the four-mirror resonant cavity; the linear polarized light beam incident to the four-mirror resonant cavity is mostly reflected, and the small part of the linear polarized light beam enters the four-mirror resonant cavity; most of linearly polarized light beams entering the four-mirror resonant cavity are circularly transmitted for multiple times along a resonant light path of the four-mirror resonant cavity and are emitted after penetrating through the optical rotation tube 6 for multiple times, and a small part of the linearly polarized light beams are emitted after directly penetrating through the four-mirror resonant cavity and the optical rotation tube 6; the emergent linearly polarized light beam part is transmitted through the analyzer 4 and then enters the detector 5; the transmitted light intensity of the linearly polarized light beam can be observed through the detector 5;
step three: rotating the analyzer 4 for a circle, and observing the change of the transmitted light intensity through the detector 5 in the rotating process, thereby determining the minimum value of the transmitted light intensity; the angle of the analyzer 4 when the transmitted light intensity reaches the minimum value is defined as the initial angle theta0Then the light source 1 is turned off;
step four: firstly, the analyzer 4 is rotated to the initial angleDegree theta0Then, the distilled water is emptied, then the glucose solution with the standard concentration is injected into the optical rotation tube 6, and then the second step is executed; at this time, since glucose has an optical rotation effect, the transmitted light intensity observed by the detector 5 is greater than the minimum value;
step five: rotating the analyzer 4, and observing the change of the transmitted light intensity through the detector 5 in the rotating process until the transmitted light intensity reaches the minimum value again; the angle of the analyzer 4 when the transmitted light intensity reaches the minimum value again is defined as a first angle theta1Then the light source 1 is turned off;
step six: according to a first angle theta1And a starting angle theta0Calculating a first rotation angle alpha1According to the first rotation angle alpha1Calculating the sensitivity k; the specific calculation formula is as follows:
α1＝θ1-θ0；
in the formula: c1Denotes the standard concentration, and C1Is a known amount;
step seven: firstly, the analyzer 4 is rotated to the initial angle theta0Emptying the glucose solution with the standard concentration, injecting the glucose solution to be detected into the optical rotation tube 6, and then executing the second step; at this time, since glucose has an optical rotation effect, the transmitted light intensity observed by the detector 5 is greater than the minimum value;
step eight: rotating the analyzer 4, and observing the change of the transmitted light intensity through the detector 5 in the rotating process until the transmitted light intensity reaches the minimum value again; the angle of the analyzer 4 when the transmitted light intensity reaches the minimum value again is defined as a second angle theta2Then the light source 1 is turned off;
step nine: according to a second angle theta2And a starting angle theta0Calculating a second rotation angle alpha2According to the second rotation angle alpha2And calculating the concentration C of the glucose solution to be detected according to the sensitivity k2(ii) a The specific calculation formula is as follows:
α2＝θ2-θ0；
in this embodiment, as shown in fig. 1, in the second step, most of the linearly polarized light beam incident to the four-mirror resonator is reflected by the first cavity mirror 301, and a small part of the linearly polarized light beam passes through the first cavity mirror 301; most of the linearly polarized light beams which penetrate through the first cavity mirror 301 circularly propagate along the rectangular resonant light path for multiple times and exit after penetrating through the optical rotation tube 6 for multiple times, and the small linearly polarized light beams exit after penetrating through the optical rotation tube 6 and the second cavity mirror 302 in sequence.
Example two
A high-sensitivity glucose concentration detection device based on a four-mirror resonant cavity comprises a light source 1, a polarizer 2, the four-mirror resonant cavity, an analyzer 4, a detector 5 and an optical rotation tube 6; wherein, the emergent end of the light source 1 is opposite to the incident end of the polarizer 2; the emergent end of the polarizer 2 is obliquely opposite to the incident end of the four-mirror resonant cavity; the emergent end of the four-mirror resonant cavity is obliquely opposite to the incident end of the analyzer 4; the emergent end of the analyzer 4 is opposite to the incident end of the detector 5; the optical rotation tube 6 is arranged on a resonant light path of the four-mirror resonant cavity.
In the present embodiment, as shown in FIG. 2, the four-mirror resonator comprises first to fourth mirrors 301-304 arranged in a rectangular shape; the first cavity mirror 301 and the third cavity mirror 303 are both semi-transparent semi-reflecting mirrors; the second cavity mirror 302 and the fourth cavity mirror 304 are both high-reflection mirrors; the front incident end of the first cavity mirror 301 is used as the incident end of the four-mirror resonant cavity; the back transmission end of the first cavity mirror 301 is diagonally opposite to the incident end of the second cavity mirror 302; the reflecting end of the second cavity mirror 302 is diagonally opposite to the incident end of the fourth cavity mirror 304; the reflection end of the fourth mirror 304 is diagonally opposite to the front incident end of the third mirror 303; the back transmission end of the third cavity mirror 303 serves as an emergent end of the four-mirror resonant cavity; the front reflection end of the third cavity mirror 303 is obliquely opposite to the back incident end of the first cavity mirror 301; the back reflection end of the first cavity mirror 301 is diagonally opposite to the incident end of the second cavity mirror 302; the first to fourth cavity mirrors 301 to 304 form an 8-shaped resonant optical path together.
The transmittance of the first cavity mirror 301 is less than 10%; the transmittance of the third cavity mirror 303 is less than that of the first cavity mirror 301; the reflectivity of the second cavity mirror 302 and the reflectivity of the fourth cavity mirror 304 are both greater than 99.5%, and the focal lengths of the two are the same.
The light source 1 adopts a laser with the output wavelength of 500 nm-760 nm; the polarizer 2 adopts a linear polaroid with an extinction ratio of more than 1: 1000; the analyzer 4 adopts an electric analyzer with a differential controller; the detector 5 comprises a photosensitive element, a data acquisition card and a computer; the incident end of the photosensitive element is used as the incident end of the detector 5; the output end of the photosensitive element is connected with the input end of the data acquisition card; the output end of the data acquisition card is connected with the input end of the computer; the optical rotation tube 6 is a quartz tube or an optical rotation tube with a calcium fluoride window.
A high-sensitivity glucose concentration detection method based on a four-mirror resonant cavity (the method is realized based on the high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity), which is realized by adopting the following steps:
the method comprises the following steps: distilled water is injected into the optical rotation tube 6;
step two: the light source 1 is turned on, and light beams emitted by the light source 1 are changed into linearly polarized light beams through the polarizer 2 and then enter the four-mirror resonant cavity; the linear polarized light beam incident to the four-mirror resonant cavity is mostly reflected, and the small part of the linear polarized light beam enters the four-mirror resonant cavity; most of linearly polarized light beams entering the four-mirror resonant cavity are circularly transmitted for multiple times along a resonant light path of the four-mirror resonant cavity and are emitted after penetrating through the optical rotation tube 6 for multiple times, and a small part of the linearly polarized light beams are emitted after directly penetrating through the four-mirror resonant cavity and the optical rotation tube 6; the emergent linearly polarized light beam part is transmitted through the analyzer 4 and then enters the detector 5; the transmitted light intensity of the linearly polarized light beam can be observed through the detector 5;
step three: rotating the analyzer 4 for a circle, and observing the change of the transmitted light intensity through the detector 5 in the rotating process, thereby determining the minimum value of the transmitted light intensity; the angle of the analyzer 4 when the transmitted light intensity reaches the minimum value is defined as the initial angle theta0Then the light source 1 is turned off;
step four: firstly, the analyzer 4 is rotated to the initial angle theta0Then, the distilled water is emptied, then the glucose solution with the standard concentration is injected into the optical rotation tube 6, and then the second step is executed; at this time, since glucose has an optical rotation effect, the transmitted light intensity observed by the detector 5 is greater than the minimum value;
step five: rotating the analyzer 4, and observing the change of the transmitted light intensity through the detector 5 in the rotating process until the transmitted light intensity reaches the minimum value again; the angle of the analyzer 4 when the transmitted light intensity reaches the minimum value again is defined as a first angle theta1Then the light source 1 is turned off;
step six: according to a first angle theta1And a starting angle theta0Calculating a first rotation angle alpha1According to the first rotation angle alpha1Calculating the sensitivity k; the specific calculation formula is as follows:
α1＝θ1-θ0；
in the formula: c1Denotes the standard concentration, and C1Is a known amount;
step seven: firstly, the analyzer 4 is rotated to the initial angle theta0Emptying the glucose solution with the standard concentration, injecting the glucose solution to be detected into the optical rotation tube 6, and then executing the second step; at this time, since glucose has an optical rotation effect, the transmitted light intensity observed by the detector 5 is greater than the minimum value;
step eight: rotating the analyzer 4, and observing the change of the transmitted light intensity through the detector 5 in the rotating process until the transmitted light intensity reaches the minimum value again; the angle of the analyzer 4 when the transmitted light intensity reaches the minimum value again is defined as a second angle theta2Then the light source 1 is turned off;
step nine: according to a second angle theta2And a starting angle theta0Calculating a second rotation angle alpha2According to the second rotation angle alpha2And sensitivityk calculating the concentration C of the glucose solution to be measured2(ii) a The specific calculation formula is as follows:
α2＝θ2-θ0；
in this embodiment, as shown in fig. 2, in the second step, most of the linearly polarized light beam incident to the four-mirror resonator is reflected by the first cavity mirror 301, and a small part of the linearly polarized light beam passes through the first cavity mirror 301; most of the linearly polarized light beams which penetrate through the first cavity mirror 301 circularly propagate along the 8-shaped resonant light path for multiple times and exit after penetrating through the optical rotation tube 6 for multiple times, and the small linearly polarized light beams which penetrate through the optical rotation tube 6 and the third cavity mirror 303 exit after being reflected by the second cavity mirror 302 and the fourth cavity mirror 304 in sequence.
While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that these are by way of example only, and that the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and scope of the invention, and these changes and modifications are within the scope of the invention.
Claims (9)
1. The utility model provides a high sensitive glucose concentration detection device based on four mirror resonant cavities which characterized in that: comprises a light source (1), a polarizer (2), a four-mirror resonant cavity, an analyzer (4), a detector (5) and an optical rotation tube (6); wherein, the emergent end of the light source (1) is opposite to the incident end of the polarizer (2); the emergent end of the polarizer (2) is obliquely opposite to the incident end of the four-mirror resonant cavity; the emergent end of the four-mirror resonant cavity is obliquely opposite to the incident end of the analyzer (4); the emergent end of the analyzer (4) is opposite to the incident end of the detector (5); the optical rotation tube (6) is arranged on a resonant light path of the four-mirror resonant cavity.
2. The high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity as claimed in claim 1, wherein: the four-mirror resonant cavity comprises first to fourth cavity mirrors (301-304) which are arranged in a rectangular shape; the first cavity mirror (301) and the second cavity mirror (302) are both semi-transparent and semi-reflective mirrors; the third cavity mirror (303) and the fourth cavity mirror (304) are both high-reflection mirrors; the front incident end of the first cavity mirror (301) is used as the incident end of the four-mirror resonant cavity; the back transmission end of the first cavity mirror (301) is obliquely opposite to the front incident end of the second cavity mirror (302); the back transmission end of the second cavity mirror (302) is used as the emergent end of the four-mirror resonant cavity; the front reflection end of the second cavity mirror (302) is obliquely opposite to the incident end of the third cavity mirror (303); the reflection end of the third cavity mirror (303) is obliquely opposite to the incidence end of the fourth cavity mirror (304); the reflection end of the fourth cavity mirror (304) is obliquely opposite to the incident end of the back of the first cavity mirror (301); the back reflection end of the first cavity mirror (301) is obliquely opposite to the front incident end of the second cavity mirror (302); the first to fourth cavity mirrors (301-304) together form a rectangular resonant light path.
3. The high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity as claimed in claim 1, wherein: the four-mirror resonant cavity comprises first to fourth cavity mirrors (301-304) which are arranged in a rectangular shape; the first cavity mirror (301) and the third cavity mirror (303) are both semi-transparent and semi-reflective mirrors; the second cavity mirror (302) and the fourth cavity mirror (304) are both high-reflection mirrors; the front incident end of the first cavity mirror (301) is used as the incident end of the four-mirror resonant cavity; the back transmission end of the first cavity mirror (301) is obliquely opposite to the incident end of the second cavity mirror (302); the reflection end of the second cavity mirror (302) is obliquely opposite to the incidence end of the fourth cavity mirror (304); the reflection end of the fourth cavity mirror (304) is obliquely opposite to the front incident end of the third cavity mirror (303); the back transmission end of the third cavity mirror (303) is used as the emergent end of the four-mirror resonant cavity; the front reflection end of the third cavity mirror (303) is obliquely opposite to the back incident end of the first cavity mirror (301); the back reflection end of the first cavity mirror (301) is obliquely opposite to the incident end of the second cavity mirror (302); the first to fourth cavity mirrors (301-304) form an 8-shaped resonant light path together.
4. The high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity as claimed in claim 2, wherein: the transmittance of the first cavity mirror (301) is less than 10%; the transmittance of the second cavity mirror (302) is less than that of the first cavity mirror (301); the reflectivity of the third cavity mirror (303) and the reflectivity of the fourth cavity mirror (304) are both more than 99.5%, and the focal lengths of the third cavity mirror and the fourth cavity mirror are the same.
5. The high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity as claimed in claim 3, wherein: the transmittance of the first cavity mirror (301) is less than 10%; the transmittance of the third cavity mirror (303) is less than that of the first cavity mirror (301); the reflectivity of the second cavity mirror (302) and the reflectivity of the fourth cavity mirror (304) are both more than 99.5%, and the focal lengths of the two are the same.
6. A four-mirror resonator-based high-sensitivity glucose concentration detection device according to claim 1, 2, 3, 4 or 5, wherein: the light source (1) adopts a laser with the output wavelength of 500 nm-760 nm; the polarizer (2) adopts a linear polaroid with an extinction ratio of more than 1: 1000; the analyzer (4) adopts an electric analyzer with a differential controller; the detector (5) comprises a photosensitive element, a data acquisition card and a computer; the incident end of the photosensitive element is used as the incident end of the detector (5); the output end of the photosensitive element is connected with the input end of the data acquisition card; the output end of the data acquisition card is connected with the input end of the computer; the optical rotation tube (6) is a quartz tube or an optical rotation tube with a calcium fluoride window.
7. A high-sensitivity glucose concentration detection method based on a four-mirror resonant cavity, which is realized based on the high-sensitivity glucose concentration detection device based on the four-mirror resonant cavity as claimed in claim 1, and is characterized in that: the method is realized by adopting the following steps:
the method comprises the following steps: injecting distilled water into the optical rotation tube (6);
step two: the light source (1) is turned on, and light beams emitted by the light source (1) are changed into linearly polarized light beams through the polarizer (2) and then enter the four-mirror resonant cavity; the linear polarized light beam incident to the four-mirror resonant cavity is mostly reflected, and the small part of the linear polarized light beam enters the four-mirror resonant cavity; most of linearly polarized light beams entering the four-mirror resonant cavity are circularly transmitted for multiple times along a resonant light path of the four-mirror resonant cavity and are emitted after penetrating through the optical rotation tube (6) for multiple times, and a small part of the linearly polarized light beams are emitted after directly penetrating through the four-mirror resonant cavity and the optical rotation tube (6); the emitted linearly polarized light beam part penetrates through the analyzer (4) and then enters the detector (5); the transmitted light intensity of the linearly polarized light beam can be observed through a detector (5);
step three: rotating the analyzer (4) for a circle, and observing the change of transmitted light intensity through the detector (5) in the rotating process, thereby determining the minimum value of the transmitted light intensity; defining the angle of the analyzer (4) when the transmitted light intensity reaches the minimum value as the initial angle theta0Then the light source (1) is switched off;
step four: firstly, the analyzer (4) is rotated to an initial angle theta0Then, the distilled water is drained, then the glucose solution with the standard concentration is injected into the optical rotation tube (6), and then the second step is executed; at this time, the transmitted light intensity observed by the detector (5) is greater than the minimum value due to the optical rotation effect of glucose;
step five: rotating the analyzer (4), and observing the change of the transmitted light intensity through the detector (5) in the rotating process until the transmitted light intensity reaches the minimum value again; the angle of the analyzer (4) when the transmitted light intensity reaches the minimum value again is defined as a first angle theta1Then the light source (1) is switched off;
step six: according to a first angle theta1And a starting angle theta0Calculating a first rotation angle alpha1According to the first rotation angle alpha1Calculating the sensitivity k; the specific calculation formula is as follows:
α1＝θ1-θ0；
in the formula: c1Denotes the standard concentration, and C1Is a known amount;
step seven: firstly, the analyzer (4) is rotated to an initial angle theta0Emptying the glucose solution with the standard concentration, injecting the glucose solution to be detected into the optical rotation tube (6), and then executing the second step; at this time, the grape is not damagedThe sugar has an optical rotation effect such that the intensity of transmitted light observed by the detector (5) is greater than a minimum value;
step eight: rotating the analyzer (4), and observing the change of the transmitted light intensity through the detector (5) in the rotating process until the transmitted light intensity reaches the minimum value again; defining the angle of the analyzer (4) when the transmitted light intensity reaches the minimum value again as a second angle theta2Then the light source (1) is switched off;
step nine: according to a second angle theta2And a starting angle theta0Calculating a second rotation angle alpha2According to the second rotation angle alpha2And calculating the concentration C of the glucose solution to be detected according to the sensitivity k2(ii) a The specific calculation formula is as follows:
α2＝θ2-θ0；
8. the method for detecting the glucose concentration based on the four-mirror resonant cavity of claim 7, wherein: in the second step, most of linearly polarized light beams entering the four-mirror resonant cavity are reflected by the first cavity mirror (301), and a small part of the linearly polarized light beams penetrate through the first cavity mirror (301); most of the linearly polarized light beams which penetrate through the first cavity mirror (301) circularly propagate along the rectangular resonant light path for multiple times and exit after penetrating through the optical rotation tube (6) for multiple times, and the small linearly polarized light beams exit after penetrating through the optical rotation tube (6) and the second cavity mirror (302) in sequence.
9. The method for detecting the glucose concentration based on the four-mirror resonant cavity of claim 7, wherein: in the second step, most of linearly polarized light beams entering the four-mirror resonant cavity are reflected by the first cavity mirror (301), and a small part of the linearly polarized light beams penetrate through the first cavity mirror (301); most of the linearly polarized light beams penetrating through the first cavity mirror (301) are circularly propagated for multiple times along the 8-shaped resonant light path and are emitted after penetrating through the optical rotation tube (6) for multiple times, and the small linearly polarized light beams are reflected by the second cavity mirror (302) and the fourth cavity mirror (304) in sequence and then are emitted after penetrating through the optical rotation tube (6) and the third cavity mirror (303) in sequence.
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