CN113481091B - Microorganism breeding and fermentation method - Google Patents

Microorganism breeding and fermentation method Download PDF

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Publication number
CN113481091B
CN113481091B CN202110936718.1A CN202110936718A CN113481091B CN 113481091 B CN113481091 B CN 113481091B CN 202110936718 A CN202110936718 A CN 202110936718A CN 113481091 B CN113481091 B CN 113481091B
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fermentation cylinder
butt joint
fermentation
breeding
joint port
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CN113481091A (en
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奥格博多
王玉兰
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Nongbo bacteria society Biotechnology Co.,Ltd.
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Nongbo Bacteria Society Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
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  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a microorganism breeding and fermentation method, which realizes the breeding and fermentation of microorganisms by using microorganism breeding and fermentation equipment; the microorganism breeding and fermenting equipment comprises: a fermentation cylinder; the fermentation cylinder is of a vertical cylinder structure; the stirring and vibrating mechanism is vertically arranged in the middle of the fermentation cylinder; the lower end of the outer wall of the fermentation cylinder is sleeved with a heating sleeve; the screening tray is of an arc-shaped structure, and is vertically and fixedly installed at the upper end of the outer wall of the fermentation cylinder. In the invention, the required flora is remained in the screening box by rotating the screw rod, one-time breeding is finished, and the environment in the fermentation cylinder is not greatly changed due to the breeding, so that the stability of the flora is ensured.

Description

Microorganism breeding and fermentation method
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a method for breeding and fermenting microorganisms.
Background
Microbial fermentation refers to a process of converting raw materials into products required by human beings through a specific metabolic pathway by using microorganisms under appropriate conditions. The existing microorganism fermentation device is mostly a fermentation device for breeding novel microorganisms in patent application CN 201821099092.3. In the process of breeding and fermenting microorganisms, a stirring mode is adopted, so that flora and mutagen are difficult to be fully fused all the time, aggregation type mixing exists, and in addition, when the flora is taken out, because the flora needs to be contacted with the outside, the temperature and the humidity of the surface layer are easy to change, so that the variation of partial flora is caused, and the breeding difference is caused.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for breeding and fermenting microorganisms, which aims to solve the problems that in the process of breeding and fermenting the microorganisms, a stirring mode is adopted, so that flora and mutagen are difficult to be fully fused all the time, aggregation type mixing exists, and in addition, when the flora is taken out, because the flora needs to be contacted with the outside, the temperature and the humidity of the surface layer are easy to change, so that partial flora is mutated, and breeding difference is caused.
The invention is achieved by the following specific technical means:
a microorganism breeding and fermentation method, which realizes the breeding and fermentation of microorganisms by using microorganism breeding and fermentation equipment; the microorganism breeding and fermenting equipment comprises:
a fermentation cylinder; the fermentation cylinder is of a vertical cylinder structure; the stirring and vibrating mechanism is vertically arranged in the middle of the fermentation cylinder; the lower end of the outer wall of the fermentation cylinder is sleeved with a heating sleeve; the screening tray is of an arc-shaped structure and is vertically and fixedly installed at the upper end of the outer wall of the fermentation cylinder; the vibration group is vertically and slidably mounted on the part of the stirring vibration mechanism, which is positioned in the inner cavity of the fermentation cylinder.
The fermentation cylinder comprises a support frame and a butt joint port A, and U-shaped support frames are respectively vertically and downwards fixedly installed at the edge positions of the bottom plane of the fermentation cylinder; a horizontal butt joint port A is formed in the position, corresponding to the screening tray, on the arc wall at the upper end of the fermentation cylinder.
Further, the stirring and oscillating mechanism comprises a servo motor, a rotating shaft and a deflector rod, and the servo motor is vertically and fixedly arranged in the middle of the bottom plane of the fermentation cylinder; the lower end of the rotating shaft is fixedly connected with a rotating shaft of the servo motor through a coupler; the driving lever is of an isosceles trapezoid structure with a short inner vertical edge and a long outer vertical edge, the inner end of the driving lever is fixedly connected with the rotating shaft, and six driving levers which are distributed annularly are mounted on the rotating shaft.
The screening tray comprises guide rods, a screw rod, a screening box and a butt joint port B, and the guide rods are respectively vertically and upwards fixedly installed at the left end and the right end of the screening tray; a screening box is vertically and slidably mounted between the upper ends of the left guide rod and the right guide rod, the opening of the screening box is upward, and the inner arc wall of the screening box is vertically and slidably tangent to the outer cylinder wall of the fermentation cylinder; the middle part of the side wall of the outer end of the screening box is vertically screwed with a screw rod, and the lower end of the screw rod is rotatably connected with the middle part of the top plane of the screening tray; the screening box is characterized in that a horizontal butt joint port B is formed in the inner arc plate of the screening box, the butt joint port B and the butt joint port A are located on the same vertical plane, the structure of the butt joint port B is consistent with that of the butt joint port A, when the screening box is located at the uppermost end position, the butt joint port B is located at the position above the butt joint port A, and the butt joint port A is located in a closed state.
Furthermore, the oscillation group comprises oscillation sleeve columns and tension springs, the oscillation sleeve columns are respectively slidably mounted on the short inclined sides of the deflector rod, and at least two oscillation sleeve columns are slidably sleeved on each short inclined side; two vibrating sleeve columns on the upper inclined short edge and the lower inclined short edge of the shifting lever are fixedly connected through a tension spring.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the screw rod is rotated to enable the screening box to move downwards along the guide rod until the butt joint port B is aligned with the butt joint port A, the upper-layer flora at the butt joint port A can flow out, the screw rod is adjusted to move upwards, the required flora is remained in the screening box, one-time breeding is finished, and the environment in the fermentation cylinder is not greatly changed due to the breeding, so that the stability of the flora is ensured.
2. In the process that the oscillating group rotates along with the deflector rod, the oscillating sleeve columns slide, and collide with each other under the action of the tension spring, so that vibration waves are generated, and the mixing effect of the flora and the mutagen is improved.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a front right upper axial view of the present invention.
Fig. 2 is a schematic diagram of the right front lower axial view structure of the invention.
Fig. 3 is a schematic top view of the present invention.
Fig. 4 is a schematic sectional view of the position a-a in fig. 3 according to the present invention.
Fig. 5 is a schematic view of the half-section separated state of the present invention.
Fig. 6 is a schematic diagram of the enlarged portion B of fig. 5 according to the present invention.
FIG. 7 is a schematic axial view showing the screening tray and the fermenter part of the present invention in a separated state.
Fig. 8 is a schematic view of the screening tray of the present invention in a partial axial view.
In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:
1. a fermentation cylinder; 101. a support frame; 102. a butt joint port A; 2. a stirring and vibrating mechanism; 201. a servo motor; 202. a rotating shaft; 203. a deflector rod; 3. heating a jacket; 4. a screening tray; 401. a guide bar; 402. a screw rod; 403. a screening box; 404. a butt joint port B; 5. a vibration group; 501. vibrating the sleeve column; 502. a tension spring.
Detailed Description
The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example (b):
as shown in figures 1 to 8:
the invention provides a microorganism breeding and fermentation method, which realizes the breeding and fermentation of microorganisms by using microorganism breeding and fermentation equipment; the microorganism breeding and fermenting equipment comprises:
a fermentation cylinder 1; the fermentation cylinder 1 is a vertical cylinder structure; the stirring and vibrating mechanism 2 is vertically arranged in the middle of the fermentation cylinder 1; the lower end of the outer wall of the fermentation cylinder 1 is sleeved with a heating sleeve 3; the screening tray 4 is of an arc-shaped structure, and the screening tray 4 is vertically and fixedly arranged at the upper end of the outer wall of the fermentation cylinder 1; the oscillation group 5 is vertically and slidably arranged on the part of the stirring oscillation mechanism 2 positioned in the inner cavity of the fermentation cylinder 1, oscillation waves can be formed through the oscillation group 5, the requirement of gradient dilution breeding of microorganisms is facilitated, and a mutagen is added in a matching manner, so that the mutagen can be fully fused;
mutagens employ alkylating agents which are substituted for the hydrogen atom of another molecule by an alkyl group are referred to as "alkylations" and such agents are referred to as alkylating agents.
The fermentation cylinder 1 comprises a support frame 101 and a butt joint A102, and U-shaped support frames 101 are respectively vertically and downwards fixedly installed at the edge positions of the bottom plane of the fermentation cylinder 1 and can be used for carrying out triangular support on the fermentation cylinder 1; the arc wall at the upper end of the fermentation cylinder 1 is provided with a horizontal butt joint port A102 at the position corresponding to the screening tray 4, and the supernatant of the fermentation cylinder 1 can be taken out through the butt joint port A102, so that the required flora can be taken out.
The stirring and oscillating mechanism 2 comprises a servo motor 201, a rotating shaft 202 and a shifting lever 203, wherein the servo motor 201 is vertically and fixedly arranged at the middle position of the bottom plane of the fermentation cylinder 1; the rotating shaft 202 is vertically and rotatably arranged at the middle part of the inner cavity of the fermentation cylinder 1, and the lower end of the rotating shaft 202 is fixedly connected with a rotating shaft of the servo motor 201 through a coupler; deflector rod 203 is the isosceles trapezoid structure of inner vertical limit short, outer vertical limit length, and deflector rod 203's the inner is connected with rotation axis 202 stationary phase, installs six deflector rods 203 that are the annular distribution altogether on rotation axis 202, and when servo motor 201 during operation, rotation axis 202 is rotatory, and accessible deflector rod 203 stirs the mixture to the culture solution in the fermentation cylinder 1 to make bacterial colony and mutagen fully fuse, improve the bacterial colony output rate.
The screening tray 4 comprises a guide rod 401, a screw rod 402, a screening box 403 and a butt joint port B404, wherein the guide rod 401 is vertically and upwards fixedly installed at the left end and the right end of the screening tray 4 respectively; a screening box 403 is vertically and slidably mounted between the upper ends of the left guide rod 401 and the right guide rod 401, the opening of the screening box 403 is upward, and the inner arc wall of the screening box 403 is vertically and slidably tangent with the outer cylinder wall of the fermentation cylinder 1; the middle part of the side wall of the outer end of the screening box 403 is vertically screwed with a screw rod 402, and the lower end of the screw rod 402 is rotatably connected with the middle part of the top plane of the screening tray 4; a horizontal butt joint port B404 is arranged on an inner arc plate of the screening box 403, the butt joint port B404 and the butt joint port A102 are positioned on the same vertical plane, the structure of the butt joint port B404 is consistent with that of the butt joint port A102, when the screening box 403 is positioned at the uppermost end position, the butt joint port B404 is positioned at the position above the butt joint port A102, the butt joint port A102 is in a closed state, when upper-layer floras needs to be taken out, the screening box 403 moves downwards along the guide rod 401 by rotating the screw rod 402 until the butt joint port B404 is aligned with the butt joint port A102, the upper-layer floras at the butt joint port A102 can flow out, the screw rod 402 is adjusted to move upwards, the needed floras remains in the screening box, one-time breeding is finished, and the environment in the fermentation cylinder 1 is not greatly changed due to breeding, so that the stability of the floras is ensured.
The oscillating group 5 comprises oscillating sleeve columns 501 and tension springs 502, the oscillating sleeve columns 501 are respectively slidably mounted on the inclined short edges of the shift lever 203, and each inclined short edge is at least slidably sleeved with two oscillating sleeve columns 501; two oscillation sleeve columns 501 on the upper inclined short edge and the lower inclined short edge of the shifting lever 203 are fixedly connected through a tension spring 502, when the oscillation group 5 rotates along with the shifting lever 203, the oscillation sleeve columns 501 slide, and under the action of the tension spring 502, the oscillation sleeve columns 501 collide with each other, so that vibration waves are generated, and the mixing effect of flora and a mutagen is improved.
In another embodiment, a plurality of vertical interfaces a102 may be provided, and the height of the screening box 403 is increased, so that the flora liquid with different heights can be selected.
The method for breeding and fermenting the microorganisms specifically comprises the following steps:
adding a flora culture solution and a mutagen from a sealing cover at the top of a fermentation cylinder 1, starting a servo motor 201 and a heating sleeve 3, adjusting the temperature to a proper temperature, rotating a rotating shaft 202 when the servo motor 201 works, and stirring and mixing the culture solution in the fermentation cylinder 1 through a shifting rod 203 so as to fully fuse the flora and the mutagen and improve the output rate of the flora, wherein in the process that a vibration group 5 rotates along with the shifting rod 203, a vibration sleeve column 501 slides, and under the action of a tension spring 502, the vibration sleeve columns 501 collide with each other to generate vibration waves, which is beneficial to improving the mixing effect of the flora and the mutagen;
when the upper flora needs to be taken out, the screw rod 402 is rotated, so that the screening box 403 moves downwards along the guide rod 401 until the butt joint port B404 is aligned with the butt joint port A102, the upper flora at the butt joint port A102 can flow out, the screw rod 402 is adjusted to move upwards, the required flora is reserved in the screening box 403, one-time breeding is finished, and the environment in the fermentation cylinder 1 is not greatly changed due to the breeding, so that the stability of the flora is ensured.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Claims (2)

1. A method for breeding and fermenting microorganisms is characterized in that: the microorganism breeding and fermentation method realizes the breeding and fermentation of microorganisms by using microorganism breeding and fermentation equipment; the microorganism breeding and fermenting equipment comprises:
a fermentation cylinder; the fermentation cylinder is of a vertical cylinder structure;
the stirring and vibrating mechanism is vertically arranged in the middle of the fermentation cylinder;
the heating sleeve is sleeved at the lower end of the outer wall of the fermentation cylinder;
the screening tray is of an arc-shaped structure and is vertically and fixedly installed at the upper end of the outer wall of the fermentation cylinder;
the vibrating group is vertically and slidably mounted on the part of the stirring vibrating mechanism, which is positioned in the inner cavity of the fermentation cylinder;
the fermentation cylinder comprises:
the edge positions of the bottom plane of the fermentation cylinder are respectively vertically and downwards fixedly provided with a U-shaped support frame;
the horizontal butt joint port A is formed in the position, corresponding to the screening tray, on the arc wall at the upper end of the fermentation cylinder;
stirring is shaken the mechanism and is included:
the servo motor is vertically and fixedly arranged at the middle position of the bottom plane of the fermentation cylinder;
the rotating shaft is vertically and rotatably arranged in the middle of the inner cavity of the fermentation cylinder, and the lower end of the rotating shaft is fixedly connected with a rotating shaft of the servo motor through a coupler;
the driving lever is of an isosceles trapezoid structure with a short vertical edge at the inner end and a long vertical edge at the outer end, the inner end of the driving lever is fixedly connected with the rotating shaft, and six driving levers which are distributed in a ring shape are mounted on the rotating shaft;
the screening tray includes:
the left end and the right end of the screening tray are respectively vertically and upwards fixedly provided with a guide rod;
the screening box is vertically and slidably arranged between the upper ends of the left guide rod and the right guide rod, the opening of the screening box is upward, and the inner arc wall of the screening box is vertically and slidably tangent to the outer cylinder wall of the fermentation cylinder;
the middle part of the side wall of the outer end of the screening box is vertically screwed with a screw rod, and the lower end of the screw rod is rotatably connected with the middle part of the top plane of the screening tray;
the horizontal butt joint port B is formed in the inner arc plate of the screening box, the butt joint port B and the butt joint port A are positioned on the same vertical plane, and the structures of the butt joint port B and the butt joint port A are consistent;
the oscillation group comprises:
the oscillating sleeve columns are respectively and slidably mounted on the inclined short edges of the deflector rod, and at least two oscillating sleeve columns are slidably sleeved on each inclined short edge;
and the two oscillating sleeve columns on the upper inclined short edge and the lower inclined short edge of the shifting lever are fixedly connected through the tension spring.
2. The method for breeding and fermenting microorganisms according to claim 1, wherein: when the screening case is located at the uppermost position, the butt joint port B is located at the position above the butt joint port A, and the butt joint port A is in a closed state.
CN202110936718.1A 2021-08-16 2021-08-16 Microorganism breeding and fermentation method Active CN113481091B (en)

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CN113549530B (en) * 2021-08-16 2024-01-19 东阳市东泉食品有限公司 Microorganism breeding and fermentation equipment

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US5061448A (en) * 1988-04-29 1991-10-29 Barnstead Thermolyne Corporation Incubator
CN206438131U (en) * 2016-12-30 2017-08-25 天津百利食品有限公司 A kind of seed selection device of excellent species
CN107881094A (en) * 2017-12-16 2018-04-06 广西华竹生物科技有限公司 Strain improvement culture apparatus
CN208869590U (en) * 2018-09-03 2019-05-17 沧州新大地生物科技有限公司 A kind of microorganism solid fermentation workshop raw material screening device
CN209549332U (en) * 2019-01-09 2019-10-29 张慧玲 A kind of pesticide blender with concussion function
CN212246999U (en) * 2020-04-24 2020-12-29 濮阳泓天威药业有限公司 Be used for bacterial breeding glass pearl structure

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Publication number Priority date Publication date Assignee Title
GB201106686D0 (en) * 2011-04-20 2011-06-01 Univ Manchester Production of succinic acid
CN108893249A (en) * 2018-06-28 2018-11-27 广西驰胜农业科技有限公司 Microbial fermentation and stirred reactor

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061448A (en) * 1988-04-29 1991-10-29 Barnstead Thermolyne Corporation Incubator
CN206438131U (en) * 2016-12-30 2017-08-25 天津百利食品有限公司 A kind of seed selection device of excellent species
CN107881094A (en) * 2017-12-16 2018-04-06 广西华竹生物科技有限公司 Strain improvement culture apparatus
CN208869590U (en) * 2018-09-03 2019-05-17 沧州新大地生物科技有限公司 A kind of microorganism solid fermentation workshop raw material screening device
CN209549332U (en) * 2019-01-09 2019-10-29 张慧玲 A kind of pesticide blender with concussion function
CN212246999U (en) * 2020-04-24 2020-12-29 濮阳泓天威药业有限公司 Be used for bacterial breeding glass pearl structure

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