CN113475744B - Method for preparing tobacco extract by using micrococcus - Google Patents
Method for preparing tobacco extract by using micrococcus Download PDFInfo
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- CN113475744B CN113475744B CN202110686470.8A CN202110686470A CN113475744B CN 113475744 B CN113475744 B CN 113475744B CN 202110686470 A CN202110686470 A CN 202110686470A CN 113475744 B CN113475744 B CN 113475744B
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- micrococcus
- tobacco extract
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
Abstract
The invention discloses a method for preparing a tobacco extract by utilizing micrococcus, which comprises the following steps: inoculating a micrococcus ZY02 single colony with the preservation number of CGMCC NO.21313 into a liquid culture medium to prepare a seed solution; preparing the seed liquid into micrococcus seeds; mixing tobacco powder with deionized water, extracting, filtering, taking filtrate, and sterilizing to obtain tobacco extract; stirring and fermenting micrococcus seeds and tobacco leaching liquor to obtain fermentation liquor; centrifuging the fermentation liquor, separating and purifying the supernatant, performing gradient elution by adopting ethanol with different purities, and collecting the eluted components to obtain a concentrated solution; adding anhydrous ethanol with the same volume into the concentrated solution, standing at low temperature for a period of time, centrifuging, and collecting supernatant to obtain alcohol precipitate; adding 1, 2-propylene glycol with the volume of 1-50% of the volume of the alcohol precipitate to obtain the tobacco extract. The method for preparing the tobacco extract by using the micrococcus has the advantages of simple process, low cost, good adding effect and the like.
Description
Technical Field
The invention relates to the field of tobacco flavors, in particular to a method for preparing a tobacco extract by utilizing micrococcus.
Background
The tobacco extract is an extremely important tobacco flavor in cigarette processing, and can endow the cigarette with more natural and full aroma. The tobacco extract is generally prepared by extracting, purifying and concentrating tobacco by adopting an organic solvent or water. The refined tobacco extract has less impurities and high quality, but has complex process and higher cost. While crude tobacco extracts are generally of high impurity and poor quality.
Moreover, the tobacco extract generates a lot of macromolecular substances such as protein, starch, pectin and the like in the extraction process, and the content of the flavor substances is basically determined by the tobacco raw materials. If a complicated separation and purification method is not adopted, the quality of the prepared tobacco extract is not ideal.
Therefore, how to provide a preparation method of a tobacco extract with simple process and low cost becomes a technical problem which needs to be solved urgently in the field.
Disclosure of Invention
The invention aims to provide a novel technical scheme of a preparation method of a tobacco extract, which has simple process and low cost.
According to a first aspect of the present invention, there is provided a method of preparing a tobacco extract using micrococcus
The method for preparing the tobacco extract by using the micrococcus is characterized by comprising the following steps of:
step (1): inoculating a micrococcus (Micrococcus Pribram) ZY02 single colony with the preservation number of CGMCC NO.21313 into a liquid culture medium, and culturing for 6-36h in a shaker at 25-40 ℃ and 200 r/min;
step (2): centrifuging the seed solution at 4 deg.C at 4000r/min for 20min, removing supernatant, adding sterile water for redissolving, and adjusting OD6002.0, preparing micrococcus seeds;
and (3): mixing tobacco powder and deionized water according to the mass ratio of 1 (2-20), extracting at 30-100 ℃ for 0.5-5 h, filtering, sterilizing the filtrate to obtain tobacco leaching liquor;
and (4): mixing micrococcus seeds with tobacco leaching liquor according to a volume ratio of 1 (50-1000), and stirring and fermenting at 25-40 deg.C and 50-300r/min for 1-5d to obtain fermentation liquor;
and (5): centrifuging the fermentation liquor, separating and purifying the supernatant, performing gradient elution by adopting ethanol with different purities, collecting elution components, and concentrating until the density is 1.145-1.155g/mL to obtain a concentrated solution;
and (6): adding absolute ethyl alcohol with the same volume into the concentrated solution, standing at low temperature for a period of time, centrifuging and taking supernatant to obtain alcohol precipitate;
and (7): adding 1, 2-propylene glycol with the volume of 1-50% of the volume of the alcohol precipitate into the alcohol precipitate to obtain the tobacco extract.
Optionally, the liquid medium in step (1) is an LB liquid medium.
Optionally, the culturing conditions in step (1) are as follows:
the temperature is 30 ℃, the shaking speed is 150r/min, and the time is 12 h.
Optionally, the step (3) is specifically as follows:
sieving tobacco powder, mixing the sieved tobacco powder with deionized water according to the mass ratio of 1 (2-20), extracting at 30-100 ℃ for 0.5-5 h, filtering, taking filtrate, and sterilizing at 121 ℃ for 10min to obtain tobacco leaching liquor.
Optionally, the step (4) is specifically as follows:
mixing micrococcus seeds with tobacco leaching liquor according to a volume ratio of 1:200, and stirring and fermenting for 2d at the temperature of 30 ℃ and at the speed of 150r/min to obtain fermentation liquor.
Optionally, the step (5) is specifically as follows:
centrifuging the fermentation liquor, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting at least two types of ethanol with different purities and volume fractions of 10-100%, collecting the eluted components, and performing vacuum concentration until the density is 1.145-1.155g/mL to obtain a concentrated solution.
Optionally, the step (5) is specifically as follows:
centrifuging the fermentation liquor at the rotation speed of 4000r/min and the temperature of 4 ℃ for 30min, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting at least two types of ethanol with different purities and volume fractions of 10-100%, collecting elution components, and concentrating the elution components at the temperature of 50 ℃ and the vacuum degree of 150mbar until the density is 1.145-1.155g/mL to obtain concentrated solution.
Optionally, the step (5) is eluted by ethanol gradient with volume fraction of 50%, 70% and 80%.
Optionally, the step (6) is specifically as follows:
adding equal volume of anhydrous ethanol into the concentrated solution, standing overnight at-20 deg.C, centrifuging, and collecting supernatant to obtain ethanol precipitate.
Optionally, the step (7) is specifically as follows:
adding 1, 2-propylene glycol with volume of 10% of that of the alcohol precipitate into the alcohol precipitate to obtain tobacco extract.
The method for preparing the tobacco extract by using the micrococcus has the advantages of simple process, low cost, good adding effect and the like.
Detailed Description
Various exemplary embodiments of the present invention will now be described in detail. It should be noted that: the relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise.
The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.
Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate.
In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values.
The method for preparing the tobacco extract by using the micrococcus comprises the following steps:
step (1): a micrococcus (Micrococcus Pribram) ZY02 with the preservation number of CGMCC NO.21313 is inoculated into a liquid culture medium and cultured for 6-36h in a shaker at 25-40 ℃ and 200 r/min.
The micrococcus (Microccceae Pribram) ZY02 is deposited in China general microbiological culture Collection center (CGMCC), the preservation number is CGMCC NO.21313, the strain name is Micrococcus, the strain number is ZY02, the classification name is Micrococcus, Microccceae Pribram, and the preservation time is 12 months and 07 days in 2020.
The liquid medium in step (1) may be an LB liquid medium.
The conditions for the culture in step (1) may be as follows:
the temperature is 30 ℃, the shaking speed is 150r/min, and the time is 12 h.
Step (2): centrifuging the seed solution at 4 deg.C at 4000r/min for 20min, removing supernatant, adding sterile water for redissolving, and adjusting OD600To 2.0, micrococcus seeds were prepared.
And (3): mixing the tobacco powder and deionized water according to the mass ratio of 1 (2-20), extracting for 0.5-5 h at 30-100 ℃, filtering, taking the filtrate, and sterilizing to obtain the tobacco leaching liquor.
The step (3) may be embodied as follows:
sieving tobacco powder, mixing the sieved tobacco powder with deionized water according to the mass ratio of 1 (2-20), extracting at 30-100 ℃ for 0.5-5 h, filtering, taking filtrate, and sterilizing at 121 ℃ for 10min to obtain tobacco leaching liquor. The tobacco powder can be high-quality tobacco powder.
Further, the step (3) is specifically as follows:
sieving tobacco powder with a 50-mesh sieve, mixing the sieved tobacco powder with deionized water according to the mass ratio of 1:10, extracting at 70 ℃ for 1.5h, filtering with a 200-mesh sieve, taking filtrate, and sterilizing at 121 ℃ for 10min to obtain tobacco leaching liquor.
And (4): mixing micrococcus seeds with tobacco leaching liquor according to a volume ratio of 1 (50-1000), and stirring and fermenting at 25-40 deg.C and 50-300r/min for 1-5d to obtain fermentation liquor.
The step (4) may be embodied as follows:
mixing micrococcus seeds with tobacco leaching liquor according to a volume ratio of 1:200, and stirring and fermenting for 2d at the temperature of 30 ℃ and at the speed of 150r/min to obtain fermentation liquor.
And (5): centrifuging the fermentation liquor, separating and purifying the supernatant, performing gradient elution by adopting ethanol with different purities, collecting the elution components, and concentrating until the density is 1.145-1.155g/mL to obtain a concentrated solution.
The step (5) may be embodied as follows:
centrifuging the fermentation liquor, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting at least two types of ethanol with different purities and volume fractions of 10-100%, collecting the eluted components, and performing vacuum concentration until the density is 1.145-1.155g/mL to obtain a concentrated solution.
Further, the step (5) is specifically as follows:
centrifuging the fermentation liquor at the rotation speed of 4000r/min and the temperature of 4 ℃ for 30min, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting at least two types of ethanol with different purities and volume fractions of 10-100%, collecting elution components, and concentrating the elution components at the temperature of 50 ℃ and the vacuum degree of 150mbar until the density is 1.145-1.155g/mL to obtain concentrated solution.
In step (5), ethanol gradients of 50%, 70% and 80% by volume may be used.
And (6): adding equal volume of anhydrous ethanol into the concentrated solution, standing at low temperature for a period of time, centrifuging, and collecting supernatant to obtain alcohol precipitate.
The step (6) may be embodied as follows:
adding equal volume of anhydrous ethanol into the concentrated solution, standing overnight at-20 deg.C, centrifuging, and collecting supernatant to obtain ethanol precipitate.
And (7): adding 1, 2-propylene glycol with the volume of 1-50% of the volume of the alcohol precipitate into the alcohol precipitate to obtain the tobacco extract.
The step (7) may be embodied as follows:
adding 1, 2-propylene glycol with volume of 10% of that of the alcohol precipitate into the alcohol precipitate to obtain tobacco extract.
The experimental procedures used in the examples below are conventional unless otherwise specified, the materials and reagents used therein are commercially available, and the equipment used in the experiments are well known to those skilled in the art without otherwise specified.
Example 1
Inoculating a single micrococcus ZY02 colony with the preservation number of CGMCC NO.21313 into an LB liquid culture medium, and culturing for 12 hours in a shaker at 30 ℃ and 150r/min to prepare a seed solution;
centrifuging the seed solution at 4 deg.C at 4000r/min for 20min, removing supernatant, adding sterile water for redissolving, and adjusting OD6002.0, preparing micrococcus seeds;
sieving tobacco powder with a 50-mesh sieve, mixing the sieved tobacco powder with deionized water according to the mass ratio of 1:10, extracting at 70 ℃ for 1.5h, filtering with a 200-mesh sieve, taking filtrate, and sterilizing at 121 ℃ for 10min to obtain tobacco leaching liquor;
mixing micrococcus seeds with a tobacco leaching solution according to a volume ratio of 1:200, and stirring and fermenting for 2d at the temperature of 30 ℃ and at the speed of 150r/min to obtain a fermentation liquid;
centrifuging the fermentation liquor at a rotation speed of 4000r/min and a temperature of 4 ℃ for 30min, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting 50%, 70% and 80% ethanol, collecting elution components, and concentrating the elution components at a temperature of 50 ℃ and a vacuum degree of 150mbar until the density is 1.145-1.155g/mL to obtain a concentrated solution;
adding anhydrous ethanol with the same volume into the concentrated solution, standing overnight at-20 deg.C, centrifuging, and collecting supernatant to obtain ethanol precipitate;
adding 1, 2-propylene glycol with the volume of 1-50% of the volume of the alcohol precipitate to obtain the tobacco extract T.
Comparative example 1
Sieving tobacco powder with a 50-mesh sieve, mixing the sieved tobacco powder with deionized water according to the mass ratio of 1:10, extracting at 70 ℃ for 1.5h, filtering with a 200-mesh sieve, taking filtrate, and sterilizing at 121 ℃ for 10min to obtain tobacco leaching liquor;
centrifuging tobacco leaching solution at rotation speed of 4000r/min and temperature of 4 deg.C for 30min, separating and purifying supernatant with HD-20 macroporous resin, gradient eluting with 50%, 70% and 80% ethanol, collecting eluate, and concentrating at 50 deg.C and vacuum degree of 150mbar to density of 1.145-1.155g/mL to obtain concentrated solution;
adding anhydrous ethanol with the same volume into the concentrated solution, standing overnight at-20 deg.C, centrifuging, and collecting supernatant to obtain ethanol precipitate;
adding 1, 2-propylene glycol with the volume of 1-50% of the volume of the alcohol precipitate to obtain the tobacco extract K.
The conventional chemical components of the tobacco extract are detected by a flow analysis method, and the flavor components of the tobacco extract are detected by GC-MS.
The conventional chemical components of the tobacco extract are shown in table 1, and it can be seen that after fermentation, the contents of total sugar and reducing sugar are obviously improved, the total nitrogen is reduced, and nicotine is basically unchanged. The total sugar and reducing sugar are increased, and the quality of the tobacco extract is improved.
TABLE 1 analysis of the content of conventional chemical components of tobacco extract (unit: mg/mL)
The flavor components of the tobacco extract are shown in table 2. As can be seen from table 2, the total content of flavor components in the fermented tobacco extract T is higher than that in the unfermented tobacco extract CK, and the content of some main flavor components such as p-hydroxyphenylethanol, 4-hydroxy- β -damascone, 9-hydroxy-4, 7-megastigmadien-3-one, benzoic acid, phenylacetic acid, phenethyl alcohol, myrcene, solanone and the like is significantly increased or newly generated, so that the quality of the fermented tobacco extract is higher.
TABLE 2 statistics of flavor components of tobacco extracts (unit: ug/mL)
Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustrative purposes only and are not intended to limit the scope of the present invention. It will be appreciated by those skilled in the art that modifications may be made to the above embodiments without departing from the scope and spirit of the invention. The scope of the invention is defined by the appended claims.
Claims (10)
1. A method for preparing tobacco extract by using micrococcus is characterized by comprising the following steps:
step (1): inoculating a micrococcus (Micrococcus Pribram) ZY02 single colony with the preservation number of CGMCC NO.21313 into a liquid culture medium, and culturing for 6-36h in a shaker at 25-40 ℃ and 200 r/min;
step (2): centrifuging the seed solution at 4 deg.C at 4000r/min for 20min, removing supernatant, adding sterile water for redissolving, and adjusting OD6002.0, preparing micrococcus seeds;
and (3): mixing tobacco powder and deionized water according to the mass ratio of 1 (2-20), extracting at 30-100 ℃ for 0.5-5 h, filtering, sterilizing the filtrate to obtain tobacco leaching liquor;
and (4): mixing micrococcus seeds with tobacco leaching liquor according to a volume ratio of 1 (50-1000), and stirring and fermenting at 25-40 deg.C and 50-300r/min for 1-5d to obtain fermentation liquor;
and (5): centrifuging the fermentation liquor, separating and purifying the supernatant, performing gradient elution by adopting ethanol with different purities, collecting elution components, and concentrating until the density is 1.145-1.155g/mL to obtain a concentrated solution;
and (6): adding absolute ethyl alcohol with the same volume into the concentrated solution, standing at low temperature for a period of time, centrifuging and taking supernatant to obtain alcohol precipitate;
and (7): adding 1, 2-propylene glycol with the volume of 1-50% of the volume of the alcohol precipitate into the alcohol precipitate to obtain the tobacco extract.
2. The method for preparing tobacco extract using micrococcus as set forth in claim 1, wherein the liquid medium in the step (1) is LB liquid medium.
3. The method for preparing tobacco extract using micrococcus as set forth in claim 1, wherein the culturing conditions in step (1) are as follows:
the temperature is 30 ℃, the shaking speed is 150r/min, and the time is 12 h.
4. The method for preparing tobacco extract by using micrococcus as claimed in claim 1, wherein the step (3) is as follows:
sieving tobacco powder, mixing the sieved tobacco powder with deionized water according to the mass ratio of 1 (2-20), extracting at 30-100 ℃ for 0.5-5 h, filtering, taking filtrate, and sterilizing at 121 ℃ for 10min to obtain tobacco leaching liquor.
5. The method for preparing tobacco extract by using micrococcus as claimed in claim 1, wherein the step (4) is as follows:
mixing micrococcus seeds with tobacco leaching liquor according to a volume ratio of 1:200, and stirring and fermenting for 2d at the temperature of 30 ℃ and at the speed of 150r/min to obtain fermentation liquor.
6. The method for preparing tobacco extract by using micrococcus as claimed in claim 1, wherein the step (5) is as follows:
centrifuging the fermentation liquor, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting at least two types of ethanol with different purities and volume fractions of 10-100%, collecting the eluted components, and performing vacuum concentration until the density is 1.145-1.155g/mL to obtain a concentrated solution.
7. The method for preparing tobacco extract by using micrococcus as claimed in claim 6, wherein the step (5) is as follows:
centrifuging the fermentation liquor at the rotation speed of 4000r/min and the temperature of 4 ℃ for 30min, separating and purifying the supernatant by adopting HD-20 macroporous resin, performing gradient elution by adopting at least two types of ethanol with different purities and volume fractions of 10-100%, collecting elution components, and concentrating the elution components at the temperature of 50 ℃ and the vacuum degree of 150mbar until the density is 1.145-1.155g/mL to obtain concentrated solution.
8. The method for preparing tobacco extract using micrococcus as claimed in claim 6, wherein the step (5) is performed by using ethanol gradient with 50%, 70% and 80% by volume fraction.
9. The method for preparing tobacco extract by using micrococcus as claimed in claim 1, wherein the step (6) is as follows:
adding equal volume of anhydrous ethanol into the concentrated solution, standing overnight at-20 deg.C, centrifuging, and collecting supernatant to obtain ethanol precipitate.
10. The method for preparing tobacco extract by using micrococcus as claimed in claim 1, wherein the step (7) is as follows:
adding 1, 2-propylene glycol with volume of 10% of that of the alcohol precipitate into the alcohol precipitate to obtain tobacco extract.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1585023A (en) * | 1977-05-06 | 1981-02-18 | Tabac Fab Reunies Sa | Process for the treating of tobacco |
| GB1585024A (en) * | 1977-05-06 | 1981-02-18 | Tabac Fab Reunies Sa | Process for treating tobacco |
| EP0024152A2 (en) * | 1979-08-20 | 1981-02-25 | Fabriques De Tabac Reunies S.A. | Continuous method of denitrating tobacco extracts |
| US4556073A (en) * | 1978-06-15 | 1985-12-03 | Brown & Williamson Tobacco Corporation | Process for reduction of nitrate content of tobacco by microbial treatment |
| US4566469A (en) * | 1978-04-25 | 1986-01-28 | Philip Morris Incorporated | Process for dissimilatory denitrification of tobacco materials |
| CN101449854A (en) * | 2008-12-30 | 2009-06-10 | 无锡华馨香精香料有限公司 | Fermentation optimization method of tobacco essence |
| CN109797114A (en) * | 2017-11-17 | 2019-05-24 | 中国科学院青岛生物能源与过程研究所 | A kind of micrococcus luteus and its application in production 2,3- butanediol |
| CN110839943A (en) * | 2019-11-27 | 2020-02-28 | 河南中烟工业有限责任公司 | Roasted sweet and fragrant tobacco extract based on biological fermentation and preparation method thereof |
-
2021
- 2021-06-21 CN CN202110686470.8A patent/CN113475744B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1585023A (en) * | 1977-05-06 | 1981-02-18 | Tabac Fab Reunies Sa | Process for the treating of tobacco |
| GB1585024A (en) * | 1977-05-06 | 1981-02-18 | Tabac Fab Reunies Sa | Process for treating tobacco |
| US4566469A (en) * | 1978-04-25 | 1986-01-28 | Philip Morris Incorporated | Process for dissimilatory denitrification of tobacco materials |
| US4556073A (en) * | 1978-06-15 | 1985-12-03 | Brown & Williamson Tobacco Corporation | Process for reduction of nitrate content of tobacco by microbial treatment |
| EP0024152A2 (en) * | 1979-08-20 | 1981-02-25 | Fabriques De Tabac Reunies S.A. | Continuous method of denitrating tobacco extracts |
| CN101449854A (en) * | 2008-12-30 | 2009-06-10 | 无锡华馨香精香料有限公司 | Fermentation optimization method of tobacco essence |
| CN109797114A (en) * | 2017-11-17 | 2019-05-24 | 中国科学院青岛生物能源与过程研究所 | A kind of micrococcus luteus and its application in production 2,3- butanediol |
| CN110839943A (en) * | 2019-11-27 | 2020-02-28 | 河南中烟工业有限责任公司 | Roasted sweet and fragrant tobacco extract based on biological fermentation and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 烟草发酵中施加微生物及制剂研究进展;宋鹏等;《安徽农业科学》;20080110(第02期);588-589,600页 * |
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