CN113475709A - Yeast extract product with fresh-keeping and taste-enhancing functions, preparation method and application thereof - Google Patents

Yeast extract product with fresh-keeping and taste-enhancing functions, preparation method and application thereof Download PDF

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Publication number
CN113475709A
CN113475709A CN202110817286.2A CN202110817286A CN113475709A CN 113475709 A CN113475709 A CN 113475709A CN 202110817286 A CN202110817286 A CN 202110817286A CN 113475709 A CN113475709 A CN 113475709A
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yeast extract
parts
stuffing
extract product
pure
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覃先武
沈硕
李库
李沛
刘雪姣
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Angel Yeast Co Ltd
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Angel Yeast Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A23L31/10Yeasts or derivatives thereof
    • A23L31/15Extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/202Aliphatic compounds
    • A23L27/2024Aliphatic compounds having oxygen as the only hetero atom
    • A23L27/2028Carboxy compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • A23L27/22Synthetic spices, flavouring agents or condiments containing amino acids containing glutamic acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/23Synthetic spices, flavouring agents or condiments containing nucleotides
    • A23L27/235Synthetic spices, flavouring agents or condiments containing nucleotides containing also amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • A23L27/35Starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3571Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Seasonings (AREA)

Abstract

The invention discloses a yeast extract product with fresh-keeping and flavor-enhancing functions, a preparation method and application thereof, and relates to the technical field of food processing. The yeast extract-based product comprises the following components: pure yeast extract, sodium glutamate, disodium flavour development nucleotide, disodium succinate, compound amino acid and maltodextrin; wherein the pure yeast extract is rich in various fresh-keeping flavor-enhancing substances such as glucan, trehalose, polypeptide, amino acid and the like; when the seasoning is applied, the seasoning and the quick-frozen stuffing are added into the stuffing and mixed uniformly, so that the usage is simple, the dosage is small, the freshness of the quick-frozen stuffing can be improved, the freshness and the taste of the stuffing are improved, the taste difference between the quick-frozen stuffing and the fresh stuffing is small, and the juicy, smooth and tender and fresh aesthetic feeling of the quick-frozen stuffing are kept.

Description

Yeast extract product with fresh-keeping and taste-enhancing functions, preparation method and application thereof
Technical Field
The invention relates to the technical field of food processing, in particular to a yeast extract product capable of improving freshness and freshness of quick-frozen stuffing and a preparation method and application thereof.
Background
With the acceleration of pace of life and the increase of household life, people have more and more demands on convenient foods, and the traditional foods with fillings, such as steamed stuffed buns, boiled dumplings, raw fried dumplings, pies and the like, gradually develop into industrially produced quick-frozen foods. The quick-frozen food is convenient and quick, has rich nutrition and is popular with people.
At present, a plurality of modifying agents are available on the market, and the aim of preserving freshness is achieved by mainly adopting more food additives as raw materials to retain water and thicken stuffing. Application publication No. CN 107594323A stuffing modifier, boiled dumplings and a preparation method thereof, wherein the modifier comprises 5-15 parts of composite phosphate, 2-5 parts of gellan gum, 2-6 parts of xanthan gum and 50-80 parts of corn starch. The pH value of meat in the stuffing is adjusted mainly through the composite phosphate, the water binding capacity of the stuffing is achieved, and meanwhile, the water is locked through the colloid, so that the function of keeping the stuffing fresh is achieved. Application publication No. CN 106036790A modifier for stuffing of wonton comprises 38 parts of wheat protein, 22 parts of D-ascorbic acid, 30 parts of konjac flour and 8 parts of thickener. The water content of the stuffing is locked by the thickening agent, the oxidation of the stuffing is reduced by adding the antioxidant, and the mouthfeel is improved. Application publication No. CN1353949 discloses a modifier for stuffing of frozen food and its application, which is characterized in that the modifier for stuffing of food contains 80-99% of starch hydrolysate and 1-20% of cold water soluble food thickener, wherein the starch hydrolysate is refined hydrolysate (maltodextrin) prepared from agricultural products containing starch or refined starch thereof. The application publication number CN101703304 discloses an antistaling agent and application thereof in stuffing, wherein the antistaling agent comprises the following components in parts by weight: 10-20 parts of butyl hydroxy anisol, 10-20 parts of sodium ascorbate, 10-15 parts of sodium polyacrylate and 2.5-5 parts of citric acid. The antioxidant is mainly used for inhibiting the oxidation of fat in the stuffing and delaying the oxidation.
Traditionally, the stuffing is directly prepared and then cooked for eating, so that quick freezing and storage do not exist in the whole process, and the change of the freshness, the taste, the mouthfeel and the like of the stuffing is small. However, the quick-frozen food needs to be subjected to quick freezing, frozen storage, water boiling or steaming or frying and reheating. In these processes, there are problems that protein denaturation, fat oxidation, juice loss, reduction in freshness and delicious taste, deterioration in water holding capacity, and deterioration in taste due to freezing storage, and the like in the filling, and thus improvement is required by some methods.
Disclosure of Invention
The technical problem solved by the invention is as follows: during the freezing storage of stuffing, protein denaturation, fat oxidation, juice loss, reduction of freshness and delicious taste, deterioration of water holding capacity and deterioration of mouthfeel can be caused.
Therefore, the invention provides a yeast extract product compounded by taking pure yeast extract (rich in natural glucan and trehalose) as a base material, which is characterized in that: the pure yeast extract with food attribute is rich in natural glucan, trehalose, polypeptide and amino acid, the glucan has the effects of water retention and moisture retention, the water retention of the filling can be well improved, the trehalose has certain inhibiting effect on protein freezing denaturation and fat oxidation, the texture and the flavor of the food are kept unchanged, meanwhile, the fishy and mutton smell of the meat is inhibited to a certain extent, and the effect of keeping fresh is achieved. The compound of the formula can provide better delicious taste by compounding various delicious substances, flavor amino acids and polypeptide and amino acid in pure yeast extract. The product prepared by the conventional uniform mixing method can be widely applied to the field of quick-frozen fillings.
In order to achieve the above objects and other related objects, the present invention provides a yeast extract product with improved freshness and delicious taste of quick-frozen stuffing.
Specifically, the invention provides the following technical scheme:
a yeast extract product with fresh-keeping and flavor-enhancing functions comprises 45-85 parts of pure yeast extract by weight; 2-20 parts of sodium glutamate; 2-20 parts of flavour nucleotide disodium; 1-20 parts of disodium succinate; 2-20 parts of compound amino acid and 2-30 parts of maltodextrin.
Wherein, the pure yeast extract comprises 18.5 to 25 weight percent of glucan, 4.7 to 30 weight percent of trehalose, 10 to 40 weight percent of polypeptide and 10 to 40 weight percent of amino acid.
Wherein the compound amino acid consists of glycine and alanine in a weight ratio of 1: 1.
Wherein, the components contained in the product are all powder, and the granularity of the prepared product is 0.8-100 meshes.
The preparation method of the pure yeast extract comprises the following steps:
a. carrying out enzymolysis on yeast under the action of nuclease, then carrying out thermal reaction at 65-75 ℃, and carrying out centrifugal separation, wherein the mass ratio of separated materials to water is 20:15-25: 12;
b. separating clear liquid, evaporating at 50-55 deg.C, and discharging at 30-45 wt%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 160-200 ℃, and the feeding pressure is 120-160 bar.
Wherein, the enzymolysis time in the step a is 12-22h, and the thermal reaction time is 0.5-1.5 h.
Wherein the feed temperature before evaporation in step b is 100-110 ℃.
Wherein the air outlet temperature of the drying tower in the step c is 85-100 ℃.
The preparation method of the yeast extract product is obtained by mixing the following raw materials in parts by weight: it comprises 45-85 parts of pure yeast extract; 2-20 parts of sodium glutamate; 2-20 parts of flavour nucleotide disodium; 1-20 parts of disodium succinate; 2-20 parts of compound amino acid and 2-30 parts of maltodextrin.
Wherein, the yeast extract product is applied in the field of food, preferably in the field of quick-frozen stuffing.
Wherein, the addition amount of the yeast extract product in the quick-frozen stuffing is 0.3-5% by weight percent.
Quick-frozen stuffing is prepared by adopting the yeast extract product.
A quick-frozen food is prepared from the yeast extract product, flour, and the raw materials of vegetables and/or meat.
The beneficial effects obtained by the invention are as follows:
the invention provides a yeast extract product for improving the freshness and the freshness of quick-frozen stuffing, which can improve the freshness of the quick-frozen stuffing, improve the freshness and the taste of the stuffing, ensure that the taste difference between the quick-frozen stuffing and the fresh stuffing is smaller, and simultaneously keep the juicy, smooth, tender and fresh aesthetic feeling of the quick-frozen stuffing. The stuffing is convenient to use, can be added into the stuffing and uniformly stirred, and has small dosage and good effect.
Information on the preservation of the strains
The strain used for preparing the pure yeast extract is Saccharomyces cerevisiae FX-2(Saccharomyces cerevisiae FX-2), which is preserved in China Center for Type Culture Collection (CCTCC) in 2016, 8 and 1 months, with the preservation number of M2016418 and the preservation address: in the Wuhan university school of eight-channel 299 # in Wuhan district, Wuhan City, Hubei province, the postal code is as follows: 430072; telephone: (027) 68752319, which is a strain that can be obtained by the prior art, and is specifically prepared, confirmed and deposited according to the method described in patent publication No. CN108220175A (application No. 201611141122.8).
Detailed Description
As mentioned above, the invention provides a yeast extract product with fresh-keeping and flavor-enhancing effects, wherein each component in the product is powdery, and the product specifically comprises 45-85 parts of pure yeast extract; 2-20 parts of sodium glutamate; 2-20 parts of flavour nucleotide disodium; 1-20 parts of disodium succinate; 2-20 parts of compound amino acid and 2-30 parts of maltodextrin. The freshness was evaluated from four aspects (acid value, peroxide value, water retention, sensory). The fresh and delicious food is subjected to effect evaluation from the sense.
In a preferred embodiment of the present invention, the pure yeast extract comprises 18.5% -25% of glucan, 4.7% -30% of trehalose, 10% -40% of polypeptide and 10% -40% of amino acid by weight%.
In a preferred embodiment of the invention, the compound amino acid is prepared from glycine and alanine in a weight ratio of 1:1, proportioning.
In a preferred embodiment of the invention, the product is made by mixing the powdered components and has a particle size of 0.8-100 mesh (screen standard GB/T6003.1-1997 wire mesh grid test specimen).
In a preferred embodiment of the present invention, the method for preparing the pure yeast extract product comprises the following steps:
a. carrying out enzymolysis on yeast for 12-22h under the action of nuclease (10 ten thousand U/g), then, carrying out centrifugal separation at 65-75 ℃ for 0.5-1.5h, wherein the mass ratio of separated material to water is 20:15-25: 12;
b. separating clear liquid and then evaporating, wherein the feeding temperature is 110 ℃ before evaporation, the evaporation temperature is 50-55 ℃, and the discharge mass concentration is 30-45%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 160-200 ℃, the feeding pressure is 120-160bar, and the air outlet temperature is 85-100 ℃.
In a preferred embodiment of the invention, the preparation method of the yeast extract product is obtained by mixing the following raw materials in parts by weight: it comprises 45-85 parts of pure yeast extract; 2-20 parts of sodium glutamate; 2-20 parts of flavour nucleotide disodium; 1-20 parts of disodium succinate; 2-20 parts of compound amino acid and 2-30 parts of maltodextrin.
In a preferred embodiment of the invention, the yeast extract product is applied to meat-based fillings, vegetable-based fillings and mixed quick-frozen fillings of meat and vegetables.
In a preferred embodiment of the invention, the addition amount of the yeast extract product in the quick-frozen stuffing is 0.3-5% by weight percent, and the yeast extract product and the seasoning are directly added into the stuffing and fully mixed when in use.
In a preferred embodiment of the invention, a quick-frozen filling is made by using the yeast extract product of the invention.
In a preferred embodiment of the present invention, a quick-frozen food product is prepared by using the yeast extract product, flour, vegetable and/or meat food material according to the present invention.
The yeast used for preparing the pure yeast extract in the examples and comparative examples of the invention is Saccharomyces cerevisiae FX-2, wherein the Saccharomyces cerevisiae FX-2(Saccharomyces cerevisiae FX-2) is preserved in China Center for Type Culture Collection (CCTCC) at 2016, 8 and 1, and has a preservation number of M2016418, and the yeast is a strain which can be obtained by the prior art, and is specifically prepared, confirmed and preserved according to the method described in the patent publication No. CN108220175A (application No. 201611141122.8).
The present invention is described in detail below with reference to specific examples, and the manufacturers of raw materials and equipment used in the examples, and the equipment and analysis method used in the product analysis are described below, wherein the chemical substances are not indicated as being of the chemical purity grade of conventional reagents. The information on the raw materials used in the examples and the experimental equipment are shown in Table 1.
Table 1 raw material information used in the examples
Figure BDA0003170622980000051
Figure BDA0003170622980000061
Example 1
50g of pure yeast extract, 15g of sodium glutamate, 4.5g of flavor nucleotide disodium, 2.5g of succinic acid disodium, 3g of compound amino acid (1.5 g of glycine and alanine respectively) and 25g of maltodextrin are uniformly mixed to obtain the yeast extract product with the granularity of 90 meshes. The specific preparation method of the pure yeast extract comprises the following steps:
a. carrying out enzymolysis on yeast for 18h under the action of nuclease (10 ten thousand U/g), then carrying out thermal reaction for 0.5h at 70 ℃, and carrying out separation, wherein the mass ratio of separated material to water is 20: 13;
b. carrying out evaporation after centrifugal separation of clear liquid, wherein the feeding temperature before evaporation is 105 ℃, the evaporation temperature is 55 ℃, and the discharge mass concentration is 40%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 180 ℃, the feeding pressure is 140bar, and the air outlet temperature is 90 ℃.
When in use, 5g of yeast extract product is added into 1kg of stuffing along with the seasoning, and the mixture is fully and uniformly mixed.
Example 2
55g of pure yeast extract, 10g of sodium glutamate, 4.5g of flavor nucleotide disodium, 2.5g of succinic acid disodium, 3g of compound amino acid (1.5 g of glycine and alanine respectively) and 25g of maltodextrin are uniformly mixed to obtain the yeast extract product with the granularity of 95 meshes. The specific preparation method of the pure yeast extract comprises the following steps:
a. carrying out enzymolysis on yeast for 20h under the action of nuclease (10 ten thousand U/g), then carrying out thermal reaction for 1h at 70 ℃, and carrying out separation, wherein the mass ratio of separated material to water is 21: 14;
b. carrying out centrifugal separation on clear liquid and then evaporating, wherein the feeding temperature before evaporation is 105 ℃, the evaporation temperature is 60 ℃, and the discharging mass concentration is 40%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 180 ℃, the feeding pressure is 140bar, and the air outlet temperature is 90 ℃.
When in use, 4g of yeast extract product is added into 1kg of stuffing along with the seasoning, and the mixture is fully and uniformly mixed.
Example 3
Taking 52.5g of pure yeast extract, 12.5g of sodium glutamate, 3.5g of flavor nucleotide disodium, 3.5g of succinic acid disodium, 3g of compound amino acid (1.5 g of glycine and alanine respectively) and 25g of maltodextrin, and uniformly mixing to obtain the yeast extract product with the granularity of 80 meshes. The specific preparation method of the pure yeast extract comprises the following steps:
a. carrying out enzymolysis on yeast for 22h under the action of nuclease (10 ten thousand U/g), then, acting for 1h at 75 ℃, and separating, wherein the mass ratio of separated materials to water is 22: 12;
b. carrying out centrifugal separation on clear liquid and then evaporating, wherein the feeding temperature before evaporation is 105 ℃, the evaporation temperature is 60 ℃, and the discharging mass concentration is 40%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 180 ℃, the feeding pressure is 140bar, and the air outlet temperature is 90 ℃.
When in use, 3.8g of yeast extract product is added into 1kg of stuffing along with the seasoning, and the mixture is fully and uniformly mixed.
Example 4
47.5g of pure yeast extract, 15g of sodium glutamate, 3.5g of flavor nucleotide disodium, 3.5g of succinic acid disodium, 3g of compound amino acid (1.5 g of glycine and alanine respectively) and 27.5g of maltodextrin are uniformly mixed to obtain the yeast extract product with the granularity of 85 meshes. The specific preparation method of the pure yeast extract comprises the following steps:
a. carrying out enzymolysis on yeast for 14h under the action of nuclease (10 ten thousand U/g), carrying out thermal reaction for 1.5h at 75 ℃, and separating, wherein the mass ratio of separated materials to water is 22: 12;
b. carrying out centrifugal separation on clear liquid and then evaporating, wherein the feeding temperature before evaporation is 105 ℃, the evaporation temperature is 60 ℃, and the discharging mass concentration is 40%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 200 ℃, the feeding pressure is 160bar, and the air outlet temperature is 90 ℃.
When in use, 6.5g of yeast extract product is added into 1kg of stuffing along with the seasoning, and the mixture is fully and uniformly mixed.
Comparative example 1:
effect comparison test using quick-frozen dumpling filling as an example, comparative example 1 is a blank control group containing no fresh-keeping flavor-enhancing yeast extract product.
Comparative example 2:
the effect comparison test taking quick-frozen dumpling filling as an example is carried out, the comparative example 2 is a commercial common yeast extract product which does not adopt a nuclease enzymolysis process, and the addition amount refers to the example 1.
Comparative example 3:
in an effect comparison test taking quick-frozen dumpling filling as an example, a yeast extract product without maltodextrin is compared with a comparative example 3, and the quick-frozen dumpling filling is specifically formed by uniformly mixing 75g of a pure yeast extract, 15g of sodium glutamate, 4.5g of flavour development nucleotide disodium, 2.5g of succinic acid disodium and 3g of compound amino acid (1.5 g of glycine and alanine respectively), wherein the addition amount refers to example 4.
TABLE 2 dumpling stuffing ratio
Figure BDA0003170622980000091
After the dumplings of examples 1-4 and comparative examples 1-3 were quickly frozen at-36 ℃ and stored at-18 ℃ for 90 days, the acid value, peroxide value, water binding capacity, and sensory evaluation (freshness, freshness (delicious taste, thick taste), filling elasticity, and juicy feeling) of the dumpling filling were determined.
TABLE 3 comparison of the freshness-retaining effects
Figure BDA0003170622980000101
The results show that the acid value of the product of the yeast extract special for fresh-keeping and flavor enhancement is at least 23.4 percent lower than that of a blank control group (comparative example 1), the peroxide value is at least 48.6 percent lower than that of the blank control group, the water retention performance is obviously provided, wherein the thawing loss is at least 20.9 percent lower than that of the blank control group, the cooking loss is at least 14.1 percent lower than that of the blank control group, and the indexes of the preferred example 4 are better than those of the comparative examples 2 and 3.
Table 4 taste effect comparison
Figure BDA0003170622980000102
Figure BDA0003170622980000111
The results show that the fresh and delicious taste and the comprehensive mouthfeel of the product are obviously improved compared with a blank control group by using the special fresh-keeping and flavor-enhancing yeast extract product, and the difference between the fresh and delicious taste and fresh stuffing is smaller in the aspects of juice, mellow taste and delicious degree; moreover, the fresh-keeping and flavor-enhancing effect of the invention is superior to that of the similar products sold in the market, and particularly, the water locking performance of the product is obviously improved by adding maltodextrin.

Claims (12)

1. A yeast extract product with fresh-keeping and flavor-enhancing functions comprises 45-85 parts of pure yeast extract by weight; 2-20 parts of sodium glutamate; 2-20 parts of flavour nucleotide disodium; 1-20 parts of disodium succinate; 2-20 parts of compound amino acid and 2-30 parts of maltodextrin.
2. The yeast extract product of claim 1, wherein the pure yeast extract comprises 18.5-25% glucan, 4.7-30% trehalose, 10-40% polypeptide, and 10-40% amino acid by weight%.
3. The yeast extract product according to claim 1 or 2, wherein the compounded amino acid is a mixture of glycine and alanine in a weight ratio of 1:1, proportioning.
4. The yeast extract product according to any of claims 1 to 3, wherein the components of the product are all in powder form, resulting in a product having a particle size of 0.8-100 mesh.
5. The yeast extract product according to any of claims 1 to 4, wherein the method for preparing the pure yeast extract comprises the following steps:
a. carrying out enzymolysis on yeast under the action of nuclease, then carrying out thermal reaction at 65-75 ℃, and carrying out centrifugal separation, wherein the mass ratio of separated materials to water is 20:15-25: 12;
b. separating clear liquid, evaporating at 50-55 deg.C, and discharging at 30-45 wt%;
c. and (3) evaporating the materials, and then carrying out spray drying to obtain the pure yeast extract, wherein the spray drying is carried out in a drying tower, the main air inlet temperature of the drying tower is 160-200 ℃, and the feeding pressure is 120-160 bar.
6. The method for preparing the purified yeast extract according to claim 5, wherein the enzymolysis time in step a is 12-22h, and the thermal reaction time is 0.5-1.5 h.
7. The method for preparing pure yeast extract according to claim 5 or 6, wherein the feeding temperature before evaporation in step b is 100-110 ℃.
8. The method for preparing the purified yeast extract according to any one of claims 5 to 7, wherein the outlet air temperature of the drying tower in the step c is 85 to 100 ℃.
9. A process for the preparation of a yeast extract product according to any one of claims 1 to 5, obtained by mixing the following raw materials in the following proportions by weight: 45-85 parts of pure yeast extract; 2-20 parts of sodium glutamate; 2-20 parts of flavour nucleotide disodium; 1-20 parts of disodium succinate; 2-20 parts of compound amino acid and 2-30 parts of maltodextrin.
10. Use of a yeast extract product according to any of claims 1 to 5 in the field of food, preferably in the field of quick frozen fillings.
11. Use of a yeast extract product according to claim 10, wherein the yeast extract product is added to the quick frozen filling in an amount of 0.3-5% by weight.
12. A quick frozen filling made by using the yeast extract product of any one of claims 1 to 5.
CN202110817286.2A 2021-07-20 2021-07-20 Yeast extract product with fresh-keeping and taste-enhancing functions, preparation method and application thereof Pending CN113475709A (en)

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