CN113466310A - Optical fiber field effect tube combined biosensor and device based on digital micro-fluidic - Google Patents
Optical fiber field effect tube combined biosensor and device based on digital micro-fluidic Download PDFInfo
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- CN113466310A CN113466310A CN202110753298.3A CN202110753298A CN113466310A CN 113466310 A CN113466310 A CN 113466310A CN 202110753298 A CN202110753298 A CN 202110753298A CN 113466310 A CN113466310 A CN 113466310A
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- 239000000835 fiber Substances 0.000 claims description 25
- 230000003287 optical effect Effects 0.000 claims description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 22
- 229910021389 graphene Inorganic materials 0.000 claims description 22
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 5
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Abstract
The present disclosure provides a digital microfluidic-based optical fiber field effect tube combined biosensor and device, including: the two end faces and the outer surface of the optical fiber are provided with conducting layers, one end face of the optical fiber is also provided with two electrodes which are respectively used as a drain electrode and a source electrode of the field effect transistor, and the other end face is provided with a conducting channel and is connected with the drain electrode and the source electrode; the photoelectric dual-mode detection device has the advantages that the same target object is detected simultaneously in the photoelectric dual-mode, the accuracy and the reliability of detection are improved, automation can be realized, the efficiency is high, the cost can be reduced, the process pollution is reduced, and the effectiveness of a detection result is improved.
Description
Technical Field
The utility model discloses the biosensor field especially relates to a optic fibre field effect transistor allies oneself with uses biosensor and device based on digital micro-fluidic.
Background
The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.
Biosensors not only promote the rapid advance of biotechnology, but also provide efficient and convenient methods for human disease diagnosis, treatment and prevention, but also face new challenges. Biosensors can be classified into electrochemical and optical based on the principle of electrochemical or optical sensing. The optical sensor can generate output characteristic optical signals (fluorescence, color change and the like) after the molecular recognition element is specifically combined with the object to be detected. The optical fiber sensor uses optical fibers as a conducting medium and collects signals for detection due to detection substances, can adapt to extremely severe environments, is free from electromagnetic interference, corrosion-resistant, safe in signal transmission, low in loss and small in size, and has wide application prospects in a plurality of research fields such as physics, chemistry, biomedicine, life science and the like. The electrochemical sensor generates electron transfer through the reaction of a detection sample and a substrate, so that current is formed, and the detection of a target object is realized through the detection current.
The traditional biosensor usually adopts a single optical or electrochemical sensing mode, and the false positive of the detection result is usually caused; on the other hand, the conventional biosensor is generally a separation device, and needs manual sample application, and the necessary steps of screening, separating, enriching and the like of a detection sample also need additional special treatment, so that the automation degree of the detection process is low.
Disclosure of Invention
In order to solve the problems, the disclosure provides a digital microfluidic-based fiber Field Effect Transistor (FET) combined biosensor and a digital microfluidic-based FET/FRET combined biosensor, which are used for realizing high-sensitivity detection of a biological sample.
In a first aspect, the present disclosure provides a fiber optic field effect transistor biosensor comprising: the optical fiber is provided with two conducting layers on two end faces and the outer surface, one end face of the optical fiber is also provided with two electrodes which are respectively used as a drain electrode and a source electrode of the field effect transistor, and the other end face is provided with a conducting channel and connected with the drain electrode and the source electrode.
In a second aspect, the present disclosure provides a method for preparing a fiber field effect transistor biosensor, comprising:
plating conductive materials on the end face and the outer surface of the optical fiber to form two conductive layers, and arranging two gold electrodes on one end face of the optical fiber to be used as a drain electrode and a source electrode of the optical fiber field effect transistor respectively;
transferring the graphene film to the other end face of the optical fiber by a wet transfer method, wherein the graphene film is used as a conductive material to connect the source electrode and the drain electrode to form a conductive channel;
and performing biochemical modification on the surface of the graphene film.
In a third aspect, the present disclosure provides a digital microfluidic-based optical fiber field effect tube combined biosensor, which includes a digital microfluidic chip and the optical fiber field effect tube biosensor of the first aspect, wherein two electrodes of the optical fiber field effect tube biosensor are respectively used as a drain electrode and a source electrode of a field effect tube, and the digital microfluidic chip is used as a gate electrode.
In a fourth aspect, the present disclosure provides a digital microfluidic based fiber field effect transistor combined biological detection apparatus, which uses the digital microfluidic based fiber field effect transistor combined biological sensor according to the third aspect for detection.
Compared with the prior art, this disclosure possesses following beneficial effect:
1. the optical fiber field effect tube biosensor comprises an optical fiber, wherein two end faces and the outer surface of the optical fiber are respectively provided with a conducting layer, one end face of the optical fiber is also provided with two electrodes which are respectively used as a drain electrode and a source electrode of a field effect tube, and the other end face is provided with a conducting channel which is connected with the drain electrode and the source electrode; the field effect tube is constructed on the optical fiber, the photoelectric double mode is adopted to simultaneously detect the same target object, the detection accuracy and reliability are improved, and the technical problem that the existing sensor for performing photoelectric detection by combining optical signals and electric signals is low in precision and difficult to detect is solved.
2. The optical fiber combined biosensor based on digital micro-fluidic changes the solid-liquid surface tension of a chip dielectric layer and liquid drops on the chip dielectric layer by applying voltage to a chip polar plate electrode, completes the operations of liquid drop generation, splitting, transportation and the like on the basis of controlling and realizing the movement of single or a plurality of discrete liquid drops on a chip plane, can realize automation, has high efficiency, can reduce cost, reduce process pollution and improve the validity of a detection result, and solves the technical problems that the biosensor and an optical sensor still have larger errors on the detection stability and precision of a biological sample and cannot meet the requirements of high-requirement experimental research and high-end biological sample detection.
3. The two are combined, the automatic separation and enrichment process of the detection sample is completed on the digital microfluidic platform, and the detection sample is automatically moved to the optical fiber field effect tube biosensor for detection, so that the integration of sample pretreatment and detection is realized.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application.
FIG. 1 is a flow chart of a method for fabricating a fiber optic field effect transistor biosensor according to the present disclosure;
FIG. 2 is a schematic diagram of a digital microfluidic-based fiber FET/FRET coupled biosensor;
fig. 3 is a detection schematic diagram of the biological detection device of the present disclosure.
The specific implementation mode is as follows:
the present disclosure is further described with reference to the following drawings and examples.
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
Interpretation of terms:
graphene: is a two-dimensional material with a single-layer sheet structure composed of carbon atoms. Due to the high specific surface area, high electric conductivity and excellent electron mobility at room temperature, the unique properties of graphene can be used for improving the sensitivity and selectivity of sensor detection, so that the graphene can be used as an ideal material for preparing electrochemical sensors and biosensors. Besides good electrical properties, graphene can generate Fluorescence Resonance Energy Transfer (FRET) with various fluorescent molecules due to the unique band gap structure of graphene, so that the optical biosensor can be conveniently constructed.
Digital microfluidic technology (DMF): is a new technology for handling tiny volume of liquid developed on chip in recent years. The method is a technology which is based on the principle that the solid-liquid surface tension of a chip dielectric layer and liquid drops on the chip dielectric layer is changed by applying voltage to a chip electrode plate electrode, and the operations of generating, splitting, transporting and the like of the liquid drops are completed by controlling and realizing the movement of single or a plurality of discrete liquid drops on a chip plane. The digital microfluidic chip has the advantages of miniaturization, integration, automation, parallelization, high efficiency, low cost and the like, and is widely applied to the fields of biochemical experiment analysis, high-tech medical treatment, optical research and the like.
Example 1
As shown in fig. 1, a fiber optic field effect transistor biosensor includes: the two end faces and the outer surface of the optical fiber are provided with conducting layers, one end face of the optical fiber is also provided with two electrodes which are respectively used as a drain electrode and a source electrode of the field effect transistor, and the other end face is provided with a conducting channel and is connected with the drain electrode and the source electrode; the conducting layer is used for transmitting the conductivity of the conducting channel, and the optical fiber is used for transmitting an optical fiber signal.
The optical fiber adopts fiber FET/FRET, the conducting layers are symmetrically arranged, a dielectric layer is arranged between the two conducting layers, an insulating area is formed between the outer surface of the optical fiber and the two conducting layers on the two end surfaces, the conducting channel at one end of the optical fiber is used for communicating the two conducting layers, and the conducting layers are formed by coating or electroplating conducting materials. As an embodiment, both end faces and the outer surface of the optical fiber are plated with a conductive material; one end face of the optical fiber plated with the conductive material is also provided with two electrodes which are respectively used as a drain electrode and a source electrode of the field effect transistor.
As one embodiment, the conductive channel is made of a graphene film, and the two lead layers are connected by the graphene film; specifically, the graphene film is arranged on one end face of the optical fiber and is used as a conductive channel to be connected with the drain electrode and the source electrode respectively.
Example 2
The present disclosure also provides a method for manufacturing a fiber field effect transistor biosensor, comprising:
step 1: plating conductive materials on the end face and the outer surface of the optical fiber to form two conductive layers, and arranging two gold electrodes on one end face of the optical fiber to be used as a drain electrode and a source electrode of the optical fiber field effect transistor respectively;
step 2: transferring the graphene film to the other end face of the optical fiber by a wet transfer method, and connecting a source electrode and a drain electrode respectively to form a conductive channel by using the graphene film as a conductive material through the conductivity of the graphene;
and step 3: and performing biochemical modification on the surface of the graphene film, specifically performing biochemical modification by PBASE transposase.
Example 3
The utility model provides an optic fibre field effect tube allies oneself with uses biosensor based on digital micro-fluidic, including digital micro-fluidic chip and the optic fibre field effect tube biosensor as in above-mentioned embodiment, two electrodes of optic fibre field effect tube biosensor are as field effect tube's source electrode and drain electrode respectively, and digital micro-fluidic chip is as the grid.
In another embodiment, the digital microfluidic chip comprises a bottom plate and a top plate, wherein the top plate covers the bottom plate, the surface of the bottom plate and/or the top plate is coated with a dielectric material and a hydrophobic material to serve as a sample cell, and the conductive channel at the top end of the optical fiber is placed in the sample cell. Specifically, a Printed Circuit Board (PCB) is used as a bottom plate, ITO glass is used as a top plate, and a dielectric material and a hydrophobic material are uniformly coated on the bottom plate and/or the top plate through a spin coater to serve as a sample cell for containing electrolyte. The sample liquid drops are separated and fused through a driving device of the microfluidic chip, and the liquid drops are moved to the fiber FET/FRET sensor after full reaction so as to be fully contacted for detection. As another embodiment, the graphene produced by the chemical vapor deposition method is transferred to cover the top end of the optical fiber by wet transfer, gold electrodes are covered, the gold electrodes on two sides of the top end of the optical fiber are respectively led out to be used as a source electrode and a drain electrode, the electrodes on the microfluidic chip are used as a grid electrode, and the top end of the optical fiber is placed in a sample cell on the electrodes of the microfluidic chip for detection.
Example 4
The disclosure also provides a digital microfluidic-based fiber field effect tube combined biological detection device, which adopts the digital microfluidic-based fiber combined biological sensor to detect;
as an implementation mode, the device also comprises a microprocessor, an analog-to-digital converter, a digital-to-analog converter and a resistance detection module;
the biosensor is used for collecting optical signals generated by the light source and transmitting the optical signals to the analog-to-digital converter, the analog-to-digital converter is used for converting the optical signals and transmitting the optical signals to the microprocessor, and the analog-to-digital sensor is also used for generating voltage signals and loading the voltage signals on a grid electrode of the biosensor; the resistance detection module is respectively connected with two ends of a drain electrode and a source electrode of the biosensor and is used for detecting the change of the conductivity of the conductive channel and transmitting the change to the microprocessor; the microprocessor is used for receiving the optical signal and processing the optical signal to realize optical signal detection, and is also used for receiving the conductivity change signal and processing the conductivity change signal to realize electric signal detection. As a further technical scheme, the biological detection device further comprises an EWOD chip driving module, an MCU and a photomultiplier tube PMT, wherein the EWOD chip driving module and the PMT are connected with the MCU, the EWOD chip driving module is used for driving a digital microfluidic chip, the photomultiplier tube PMT is used for enhancing photoelectric signals, and the MCU is used for controlling other modules.
The resistance detection module is a bridge type balance circuit.
Although the present disclosure has been described with reference to specific embodiments, it should be understood that the scope of the present disclosure is not limited thereto, and those skilled in the art will appreciate that various modifications and changes can be made without departing from the spirit and scope of the present disclosure.
Claims (10)
1. A fiber optic field effect transistor biosensor, comprising: the optical fiber is provided with two conducting layers on two end faces and the outer surface, one end face of the optical fiber is also provided with two electrodes which are respectively used as a drain electrode and a source electrode of the field effect transistor, and the other end face is provided with a conducting channel and connected with the drain electrode and the source electrode.
2. The fiber optic field effect transistor biosensor of claim 1, wherein the conductive channel is a graphene film, and the surface of the graphene film is biologically modified.
3. The fiber optic field effect transistor biosensor of claim 1, wherein the graphene film surface is biochemically modified by a transposase.
4. The dffet biosensor according to claim 1, wherein the conductive layer has two positions, the two conductive layers are symmetrically disposed, and the dielectric layer is disposed between the two conductive layers.
5. The dffet biosensor according to claim 1, wherein the conductive layer is coated or plated with a conductive material.
6. A preparation method of a fiber field effect transistor biosensor is characterized by comprising the following steps:
plating conductive materials on the end face and the outer surface of the optical fiber to form two conductive layers, and arranging two gold electrodes on one end face of the optical fiber to be used as a drain electrode and a source electrode of the optical fiber field effect transistor respectively;
transferring the graphene film to the other end face of the optical fiber by a wet transfer method, wherein the graphene film is used as a conductive material to connect the source electrode and the drain electrode to form a conductive channel;
and performing biochemical modification on the surface of the graphene film.
7. A biosensor used in combination with an optical fiber field effect tube based on digital microfluidics, which is characterized by comprising a digital microfluidics chip and the optical fiber field effect tube biosensor as claimed in any one of claims 1 to 5, wherein two electrodes of the optical fiber field effect tube biosensor are respectively used as a drain electrode and a source electrode of the field effect tube, and the digital microfluidics chip is used as a grid electrode.
8. The digital microfluidic-based fiber field effect transistor biosensor in combination according to claim 7, wherein the digital microfluidic chip comprises a bottom plate and a top plate, the top plate covers the bottom plate, the surface of the bottom plate and/or the top plate is coated with a dielectric material and a hydrophobic material to serve as a sample cell, and the conductive channel at the top end of the optical fiber is placed in the sample cell.
9. A digital microfluidic-based fiber field effect transistor (effet) -based biological detection device, which is characterized in that the digital microfluidic-based fiber field effect transistor (effet) -based biological sensor according to any one of claims 7 to 8 is used for detection.
10. The digital microfluidic-based fiber optic field effect transistor (effet) -based biodetection device of claim 9, further comprising a microprocessor, an analog-to-digital converter, a digital-to-analog converter, and a resistance detection module;
the biosensor is used for collecting optical signals generated by the light source and transmitting the optical signals to the analog-to-digital converter, and the analog-to-digital converter is used for converting the optical signals and transmitting the optical signals to the microprocessor;
the resistance detection module is respectively connected with two ends of a drain electrode and a source electrode of the biosensor and is used for detecting the change of the conductivity of the conductive channel and transmitting the change to the microprocessor;
the microprocessor is used for receiving the optical signal and processing the optical signal to realize optical signal detection, and is also used for receiving the conductivity change signal and processing the conductivity change signal to realize electric signal detection.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114371208A (en) * | 2021-11-30 | 2022-04-19 | 山东师范大学 | Biosensor manufacturing and detecting method, biosensor and photo-electromagnetic system |
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|---|---|---|---|---|
| US20170176379A1 (en) * | 2014-03-28 | 2017-06-22 | The Board Of Trustees Of The University Of Illinois | Label Free Analyte Detection by Electronic Desalting and Field Effect Transistors |
| CN107488709A (en) * | 2017-06-23 | 2017-12-19 | 山东师范大学 | Optical fiber FET biology sensor, biological monitor and method |
| CN111521662A (en) * | 2020-04-23 | 2020-08-11 | 浙江大学 | Sensing chip and manufacturing method thereof |
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- 2021-07-02 CN CN202110753298.3A patent/CN113466310A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170176379A1 (en) * | 2014-03-28 | 2017-06-22 | The Board Of Trustees Of The University Of Illinois | Label Free Analyte Detection by Electronic Desalting and Field Effect Transistors |
| CN107488709A (en) * | 2017-06-23 | 2017-12-19 | 山东师范大学 | Optical fiber FET biology sensor, biological monitor and method |
| CN111521662A (en) * | 2020-04-23 | 2020-08-11 | 浙江大学 | Sensing chip and manufacturing method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114371208A (en) * | 2021-11-30 | 2022-04-19 | 山东师范大学 | Biosensor manufacturing and detecting method, biosensor and photo-electromagnetic system |
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