CN113466226A - Test paper for detecting sesame oil purity, preparation method and detection method - Google Patents
Test paper for detecting sesame oil purity, preparation method and detection method Download PDFInfo
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- CN113466226A CN113466226A CN202110940260.7A CN202110940260A CN113466226A CN 113466226 A CN113466226 A CN 113466226A CN 202110940260 A CN202110940260 A CN 202110940260A CN 113466226 A CN113466226 A CN 113466226A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention provides a sesame oil purity detection method, test paper for detecting sesame oil purity and a preparation method thereof, wherein the detection method comprises the following steps: providing a test paper and a detection reagent, dripping the detection reagent on the test paper, dripping the sesame oil sample on the test paper, observing and developing color to judge the purity of the sesame oil sample, wherein the test paper has D-fructose and triton-100, the detection reagent is concentrated hydrochloric acid, and the sesame oil sample solution is not subjected to pretreatment. The invention establishes a semi-quantitative rapid detection method for accurately detecting the purity of sesame oil, and adopts D-fructose to prepare the test paper, so that the color developing agent is more stable, the reaction is faster, and the color development is deeper. The test paper can be used for carrying out quick, convenient and quick semi-quantitative analysis and detection on a sample to be detected, has the advantages of simplicity and convenience in operation, low price, convenience in carrying, certain sensitivity and specificity, no need of a specific precise instrument, and suitability for on-site quick detection.
Description
The application has application date of 2019, 26.02 and application number of 201910139709.2 and is named asA kind of Test paper for detecting purity of sesame oil and preparation method thereofIn (2) toDivisional application of the application.
Technical Field
The invention belongs to the field of food safety detection, and particularly relates to test paper for detecting sesame oil purity, a preparation method and a detection method.
Background
The sesame oil is oil prepared by using sesame as a raw material, and other edible oil and non-edible oil cannot be mixed, and any essence and spice cannot be added (GB 8233 + 2008 sesame oil). Since sesame oil is more expensive than other edible oils, many illegal merchants are motivated to incorporate other inexpensive edible oils such as rapeseed oil, cottonseed oil, palm oil, etc., and even some non-edible oils such as mineral oil, tung oil, etc., into sesame oil to obtain a riot. These behaviors not only cause harm to the interests and psychology of consumers, but also harm the moral integrity of the society, the normal operation of the market and the food safety, and further harm the benign development of the sesame and sesame oil industry. Therefore, the research on the sesame oil purity detection method is increased, and a scientific, rapid, accurate and effective identification technology for the sesame oil purity is established, so that the cost and difficulty of counterfeiting and selling are effectively increased, the negative influence and loss of enterprises caused by counterfeit products are reduced, and the healthy and rapid development of the sesame industry is promoted.
The existing method for detecting the purity of sesame oil mainly comprises the following steps: sensory identification, physicochemical property detection, spectrophotometry, color development method, chromatography, and spectrum detection. The color developing agent method is mainly a method for qualitatively and quantitatively detecting a colored substance by using a characteristic substance in grease to react with a color developing agent and a standard contrast, and is mainly divided into a concentrated sulfuric acid color developing method, a Villavecehia method and a Boudouin method. The concentrated sulfuric acid color development method cannot be used for paper test; the Villavecehia method adopted by GB/T5539-2008 "qualitative test of grain and oil inspection oil and fat" is based on the principle that sesamin special components in sesame oil react with furfural in the presence of hydrochloric acid to generate red compounds so as to judge whether the oil contains the sesame oil or not, but the furfural is unstable in air and has a low flash point; DB 34/T924-2009 colorimetric assay for sesame oil purity adopts the Boudouin method, which utilizes characteristic reaction of sesamol in sesame oil and sucrose hydrochloric acid solution to generate a red compound to judge whether the oil contains the sesame oil, but the sucrose hydrochloric acid solution developer has poor stability, harsh detection conditions and poor detection precision and accuracy. The invention provides test paper for detecting the purity of sesame oil, and by adopting the test paper, a detector does not need to prepare a sample standard solution when using the test paper, and can directly sample and detect the sample to realize the semi-quantitative analysis result with rapidness, convenience and higher accuracy on the purity of the sesame oil of the sample to be detected, thereby greatly improving the detection efficiency.
Disclosure of Invention
The test paper can carry out quick, convenient and high-accuracy semi-quantitative analysis and detection on a sample to be detected, is simple and convenient to operate, low in price, convenient to carry, and suitable for quick detection on site, and has certain sensitivity and specificity, and a specific and precise instrument is not needed.
A preparation method of test paper for detecting sesame oil purity is characterized by comprising the following steps:
the first step is as follows: accurately weighing 5g-30g D-fructose, dissolving with deionized water and fixing the volume to 100 mL;
the second step is that: accurately weighing 0.1g-3g of triton-100, dissolving with the D-fructose solution prepared in the first step, and fixing the volume to 100 mL;
the third step: soaking the large piece of absorbent paper in the mixed reagent prepared in the second step for a period of time, taking out the large piece of absorbent paper, and then placing the large piece of absorbent paper in a blast drying oven for drying treatment;
the fourth step: cutting the large test paper processed in the third step into test paper of 0.8cm by 0.8cm, and sticking the test paper on a hard bottom plate to obtain the test paper for detecting the purity of the sesame oil;
the principle of the Boudouin method: according to the principle of the invention, D-fructose is used to replace sucrose, and the sesame oil and sesamol in the sesame oil undergo color development reaction in the presence of concentrated hydrochloric acid, and the sesame oil purity is detected according to the semi-quantitative detection of the color depth, so that the experiment proves that the D-fructose reacts with the sesame oil more rapidly, the color development is deeper, and the reaction is obviously superior to that of the sucrose.
The invention has the beneficial effects that: the sesame oil purity detection test paper overcomes the defects of insufficient and simple operation, long test time, low sensitivity and the like of the conventional sesame oil purity detection, and provides workers, merchants, consumers and the like with more convenient and rapid use, safer operation, higher sensitivity and higher accuracy. According to the method, a relatively accurate semi-quantitative quick measurement method for the purity of the sesame oil is established according to the Boudouin method, and a sesame oil sample is directly detected without pretreatment and directly observed to obtain a result; no specific precise instrument is needed in the detection process; the operation is simple and convenient, professional operation is not needed, and the sensitivity is high; the test paper prepared from the D-fructose is used for replacing sucrose, so that the color developing agent is more stable, the reaction is quicker, and the color development is darker.
Detailed Description
A preparation method of test paper for detecting sesame oil purity comprises the following steps:
the first step is as follows: accurately weighing 15g D-fructose, dissolving with deionized water and fixing the volume to 100 mL;
the second step is that: accurately weighing 1g of triton-100, dissolving with the D-fructose solution prepared in the first step, and fixing the volume to 100 mL;
the third step: soaking the large piece of absorbent paper in the mixed reagent prepared in the second step for a period of time, taking out the large piece of absorbent paper, and then placing the large piece of absorbent paper in a blast drying oven for drying treatment;
the fourth step: cutting the large test paper processed in the third step into test paper of 0.8cm by 0.8cm, and sticking the test paper on a hard bottom plate to obtain the test paper for detecting the purity of the sesame oil;
secondly, a detection method of the sesame oil purity rapid detection test paper comprises the following steps:
a piece of sesame oil purity rapid detection test paper is horizontally placed on a table, a drop of detection reagent (concentrated HCL) is added to the center position of a detection area of the detection test paper by using a 1mL dropper, a drop of sample liquid is added to the same position by using another 1mL dropper (a liquid transfer gun can also be used), and the result can be judged after observation and color development after 6-7 min.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A method for detecting the purity of sesame oil is used for detecting the purity of a sesame oil sample liquid, and is characterized by comprising the following steps: providing a test paper and a detection reagent, dripping the detection reagent on the test paper, dripping the sesame oil sample on the test paper, observing and developing color to judge the purity of the sesame oil sample, wherein the test paper has D-fructose and triton-100, the detection reagent is concentrated hydrochloric acid, and the sesame oil sample solution is not subjected to pretreatment.
2. The detection method according to claim 1, wherein color development is observed after 6 to 7min after the sesame oil sample is dropped onto the test paper.
3. The assay of claim 1 wherein the detection reagent is present in the same amount as the sesame oil sample.
4. The assay method of claim 3, wherein a drop of said assay reagent is applied to said test strip with a 1ml dropper, and a drop of said sesame oil-like solution is applied to said test strip with a 1ml dropper.
5. The detection method as set forth in claim 4, wherein a dropper used for dropping the detection reagent is different from a dropper used for dropping the sesame oil-like liquid.
6. The assay of claim 1 wherein said detection reagent is dropped onto the same location on said test strip as said sesame oil sample.
7. The detection method of claim 1, wherein no specific precision instrument is used.
8. A preparation method of test paper for detecting sesame oil purity is characterized in that the preparation method comprises the following steps:
the first step is as follows: accurately weighing 5g-30g of D-fructose, dissolving with deionized water and fixing the volume to 100 mL;
the second step is that: accurately weighing 0.1g-3g of triton-100, dissolving with the D-fructose solution prepared in the first step, and fixing the volume to 100 mL;
the third step: soaking the large piece of absorbent paper in the mixed reagent prepared in the second step for a period of time, taking out the large piece of absorbent paper, and then placing the large piece of absorbent paper in a blast drying oven for drying treatment;
the fourth step: and cutting the large piece of absorbent paper processed in the third step, and sticking the cut large piece of absorbent paper on a hard bottom plate to obtain the test paper for detecting the purity of the sesame oil.
9. The test method according to claim 8, wherein the large sheet of absorbent paper is cut into 0.8cm by 0.8cm test strips.
10. A test paper for detecting the purity of sesame oil, wherein when the test paper detects the purity of a sesame oil sample solution, the sesame oil sample solution is not subjected to pretreatment, and the test paper is prepared by the preparation method according to any one of claims 8 or 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202110940260.7A CN113466226A (en) | 2019-02-26 | 2019-02-26 | Test paper for detecting sesame oil purity, preparation method and detection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910139709.2A CN111610182B (en) | 2019-02-26 | 2019-02-26 | Detection method of test paper for detecting purity of sesame oil |
| CN202110940260.7A CN113466226A (en) | 2019-02-26 | 2019-02-26 | Test paper for detecting sesame oil purity, preparation method and detection method |
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| CN112858281A (en) * | 2021-03-02 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Integrated rapid detection tube and method for rapidly detecting purity of sesame oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104297234A (en) * | 2014-10-11 | 2015-01-21 | 北京华益精点生物技术有限公司 | Preparation methods of color developing agent and test paper for testing boric acid and borax |
| CN106442506A (en) * | 2016-10-14 | 2017-02-22 | 湖南省食品质量监督检验研究院 | Process for rapidly detecting raw-cooked degree of soybean milk |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483314A (en) * | 2014-12-25 | 2015-04-01 | 桂林中辉科技发展有限公司 | Method for improvingthermal stability of test strips for detecting peroxide value of cooking oil |
| CN109258837A (en) * | 2018-09-18 | 2019-01-25 | 安徽阜阳宝鼎粮油有限责任公司 | A kind of formula of drinkable sesame oil |
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- 2019-02-26 CN CN201910139709.2A patent/CN111610182B/en active Active
- 2019-02-26 CN CN202110940260.7A patent/CN113466226A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104297234A (en) * | 2014-10-11 | 2015-01-21 | 北京华益精点生物技术有限公司 | Preparation methods of color developing agent and test paper for testing boric acid and borax |
| CN106442506A (en) * | 2016-10-14 | 2017-02-22 | 湖南省食品质量监督检验研究院 | Process for rapidly detecting raw-cooked degree of soybean milk |
Non-Patent Citations (2)
| Title |
|---|
| PIERRE BUDOWSKI等: "THE CHEMICAL AND PHYSIOLOGICAL PROPERTIES OF SESAME OIL", 《CHEMICAL REVIEWS》 * |
| 项锦欣等: "芝麻油掺伪检测方法研究进展", 《重庆工学院学报(自然科学版)》 * |
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| CN111610182A (en) | 2020-09-01 |
| CN111610182B (en) | 2021-09-07 |
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