CN111610182B - Detection method of test paper for detecting purity of sesame oil - Google Patents
Detection method of test paper for detecting purity of sesame oil Download PDFInfo
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- CN111610182B CN111610182B CN201910139709.2A CN201910139709A CN111610182B CN 111610182 B CN111610182 B CN 111610182B CN 201910139709 A CN201910139709 A CN 201910139709A CN 111610182 B CN111610182 B CN 111610182B
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- sesame oil
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- 239000008159 sesame oil Substances 0.000 title claims abstract description 39
- 235000011803 sesame oil Nutrition 0.000 title claims abstract description 39
- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 238000012360 testing method Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 17
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 claims abstract description 10
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 10
- 238000011161 development Methods 0.000 claims abstract description 10
- 239000002250 absorbent Substances 0.000 claims description 10
- 230000002745 absorbent Effects 0.000 claims description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 6
- 229930006000 Sucrose Natural products 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 238000012764 semi-quantitative analysis Methods 0.000 abstract description 3
- 229960004793 sucrose Drugs 0.000 abstract 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 239000003921 oil Substances 0.000 description 5
- 235000019198 oils Nutrition 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000207961 Sesamum Species 0.000 description 3
- 235000003434 Sesamum indicum Nutrition 0.000 description 3
- 239000008157 edible vegetable oil Substances 0.000 description 3
- UIOFUWFRIANQPC-JKIFEVAISA-N Floxacillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C1=C(C)ON=C1C1=C(F)C=CC=C1Cl UIOFUWFRIANQPC-JKIFEVAISA-N 0.000 description 2
- LUSZGTFNYDARNI-UHFFFAOYSA-N Sesamol Natural products OC1=CC=C2OCOC2=C1 LUSZGTFNYDARNI-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000010461 other edible oil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- PEYUIKBAABKQKQ-AFHBHXEDSA-N (+)-sesamin Chemical compound C1=C2OCOC2=CC([C@H]2OC[C@H]3[C@@H]2CO[C@@H]3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-AFHBHXEDSA-N 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 235000019484 Rapeseed oil Nutrition 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- PEYUIKBAABKQKQ-UHFFFAOYSA-N epiasarinin Natural products C1=C2OCOC2=CC(C2OCC3C2COC3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- VRMHCMWQHAXTOR-CMOCDZPBSA-N sesamin Natural products C1=C2OCOC2=CC([C@@H]2OC[C@@]3(C)[C@H](C=4C=C5OCOC5=CC=4)OC[C@]32C)=C1 VRMHCMWQHAXTOR-CMOCDZPBSA-N 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002383 tung oil Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses test paper for detecting sesame oil purity and a preparation method thereof, belonging to the field of food safety detection. A semi-quantitative rapid detection method for accurately detecting the purity of the sesame oil is established by adopting the Boudouin method principle, and D-fructose is selected to prepare test paper to replace cane sugar, so that the color developing agent is more stable, the reaction is faster, and the color development is darker. The test paper can be used for carrying out quick, convenient and quick semi-quantitative analysis and detection on a sample to be detected, has the advantages of simplicity and convenience in operation, low price, convenience in carrying, certain sensitivity and specificity, no need of a specific precise instrument, and suitability for on-site quick detection.
Description
Technical Field
The invention belongs to the field of food safety detection, and particularly relates to a detection method of test paper for detecting sesame oil purity.
Background
The sesame oil is oil prepared by using sesame as a raw material, and other edible oil and non-edible oil cannot be mixed, and any essence and spice cannot be added (GB 8233 + 2008 sesame oil). Since sesame oil is more expensive than other edible oils, many illegal merchants are motivated to incorporate other inexpensive edible oils such as rapeseed oil, cottonseed oil, palm oil, etc., and even some non-edible oils such as mineral oil, tung oil, etc., into sesame oil to obtain a riot. These behaviors not only cause harm to the interests and psychology of consumers, but also harm the moral integrity of the society, the normal operation of the market and the food safety, and further harm the benign development of the sesame and sesame oil industry. Therefore, the research on the sesame oil purity detection method is increased, and a scientific, rapid, accurate and effective identification technology for the sesame oil purity is established, so that the cost and difficulty of counterfeiting and selling are effectively increased, the negative influence and loss of enterprises caused by counterfeit products are reduced, and the healthy and rapid development of the sesame industry is promoted.
The existing method for detecting the purity of sesame oil mainly comprises the following steps: sensory identification, physicochemical property detection, spectrophotometry, color development method, chromatography, and spectrum detection. The color developing agent method is mainly a method for qualitatively and quantitatively detecting a colored substance by using a characteristic substance in grease to react with a color developing agent and a standard contrast, and is mainly divided into a concentrated sulfuric acid color developing method, a Villavecehia method and a Boudouin method. The concentrated sulfuric acid color development method cannot be used for paper test; the Villavecehia method adopted by GB/T5539-2008 "qualitative test of grain and oil inspection oil and fat" is based on the principle that sesamin special components in sesame oil react with furfural in the presence of hydrochloric acid to generate red compounds so as to judge whether the oil contains the sesame oil or not, but the furfural is unstable in air and has a low flash point; DB 34/T924-2009 colorimetric assay for sesame oil purity adopts the Boudouin method, which utilizes characteristic reaction of sesamol in sesame oil and sucrose hydrochloric acid solution to generate a red compound to judge whether the oil contains the sesame oil, but the sucrose hydrochloric acid solution developer has poor stability, harsh detection conditions and poor detection precision and accuracy. The invention provides test paper for detecting the purity of sesame oil, and by adopting the test paper, a detector does not need to prepare a sample standard solution when using the test paper, and can directly sample and detect the sample to realize the semi-quantitative analysis result with rapidness, convenience and higher accuracy on the purity of the sesame oil of the sample to be detected, thereby greatly improving the detection efficiency.
Disclosure of Invention
The test paper can carry out quick, convenient and high-accuracy semi-quantitative analysis and detection on a sample to be detected, is simple and convenient to operate, low in price, convenient to carry, and suitable for quick detection on site, and has certain sensitivity and specificity, and a specific and precise instrument is not needed.
A preparation method of test paper for detecting sesame oil purity is characterized by comprising the following steps:
the first step is as follows: accurately weighing 5g-30g D-fructose, dissolving with deionized water and fixing the volume to 100 mL;
the second step is that: accurately weighing 0.1g-3g of triton-100, dissolving with the D-fructose solution prepared in the first step, and fixing the volume to 100 mL;
the third step: soaking the large piece of absorbent paper in the mixed reagent prepared in the second step for a period of time, taking out the large piece of absorbent paper, and then placing the large piece of absorbent paper in a blast drying oven for drying treatment;
the fourth step: cutting the large test paper processed in the third step into test paper of 0.8cm by 0.8cm, and sticking the test paper on a hard bottom plate to obtain the test paper for detecting the purity of the sesame oil;
the principle of the Boudouin method: according to the principle of the invention, D-fructose is used to replace sucrose, and the sesame oil and sesamol in the sesame oil undergo color development reaction in the presence of concentrated hydrochloric acid, and the sesame oil purity is detected according to the semi-quantitative detection of the color depth, so that the experiment proves that the D-fructose reacts with the sesame oil more rapidly, the color development is deeper, and the reaction is obviously superior to that of the sucrose.
The invention has the beneficial effects that: the sesame oil purity detection test paper overcomes the defects of insufficient and simple operation, long test time, low sensitivity and the like of the conventional sesame oil purity detection, and provides workers, merchants, consumers and the like with more convenient and rapid use, safer operation, higher sensitivity and higher accuracy. According to the method, a relatively accurate semi-quantitative quick measurement method for the purity of the sesame oil is established according to the Boudouin method, and a sesame oil sample is directly detected without pretreatment and directly observed to obtain a result; no specific precise instrument is needed in the detection process; the operation is simple and convenient, professional operation is not needed, and the sensitivity is high; the test paper prepared from the D-fructose is used for replacing sucrose, so that the color developing agent is more stable, the reaction is quicker, and the color development is darker.
Detailed Description
A preparation method of test paper for detecting sesame oil purity comprises the following steps:
the first step is as follows: accurately weighing 15g D-fructose, dissolving with deionized water and fixing the volume to 100 mL;
the second step is that: accurately weighing 1g of triton-100, dissolving with the D-fructose solution prepared in the first step, and fixing the volume to 100 mL;
the third step: soaking the large piece of absorbent paper in the mixed reagent prepared in the second step for a period of time, taking out the large piece of absorbent paper, and then placing the large piece of absorbent paper in a blast drying oven for drying treatment;
the fourth step: cutting the large test paper processed in the third step into test paper of 0.8cm by 0.8cm, and sticking the test paper on a hard bottom plate to obtain the test paper for detecting the purity of the sesame oil;
secondly, a detection method of the sesame oil purity rapid detection test paper comprises the following steps:
a piece of sesame oil purity rapid detection test paper is horizontally placed on a table, a drop of detection reagent (concentrated HCL) is added to the center position of a detection area of the detection test paper by using a 1mL dropper, a drop of sample liquid is added to the same position by using another 1mL dropper (a liquid transfer gun can also be used), and the result can be judged after observation and color development after 6-7 min.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (1)
1. A detection method of semi-quantitative quick test paper for sesame oil purity, which is characterized in that,
the method comprises the following steps:
placing a piece of sesame oil purity rapid detection test paper on a table, adding a drop of detection reagent to the central position of a semi-quantitative rapid detection paper detection area by using a 1mL dropper, adding a drop of sesame oil sample liquid to the central position by using another 1mL dropper, observing color development after 6-7min, and interpreting the result;
the detection reagent is concentrated HCl, the sesame oil sample liquid is directly detected without pretreatment, a detection result is obtained by direct observation, and a specific precise instrument is not needed in the detection process;
the semi-quantitative quick test paper is prepared by the following steps:
the first step is as follows: accurately weighing 5g-30g of D-fructose, dissolving with deionized water and fixing the volume to 100 mL;
the second step is that: accurately weighing 0.1g-3g of triton-100, dissolving with the D-fructose solution prepared in the first step, and fixing the volume to 100 mL;
the third step: soaking the large piece of absorbent paper in the mixed reagent prepared in the second step for a period of time, taking out the large piece of absorbent paper, and then placing the large piece of absorbent paper in a blast drying oven for drying treatment;
the fourth step: cutting the large piece of absorbent paper processed in the third step into test paper of 0.8cm by 0.8cm, and sticking the test paper on a hard bottom plate to obtain the test paper for detecting the purity of the sesame oil.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110940260.7A CN113466226A (en) | 2019-02-26 | 2019-02-26 | Test paper for detecting sesame oil purity, preparation method and detection method |
| CN201910139709.2A CN111610182B (en) | 2019-02-26 | 2019-02-26 | Detection method of test paper for detecting purity of sesame oil |
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| CN201910139709.2A CN111610182B (en) | 2019-02-26 | 2019-02-26 | Detection method of test paper for detecting purity of sesame oil |
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| CN202110940260.7A Division CN113466226A (en) | 2019-02-26 | 2019-02-26 | Test paper for detecting sesame oil purity, preparation method and detection method |
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| CN111610182A CN111610182A (en) | 2020-09-01 |
| CN111610182B true CN111610182B (en) | 2021-09-07 |
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| CN201910139709.2A Active CN111610182B (en) | 2019-02-26 | 2019-02-26 | Detection method of test paper for detecting purity of sesame oil |
| CN202110940260.7A Pending CN113466226A (en) | 2019-02-26 | 2019-02-26 | Test paper for detecting sesame oil purity, preparation method and detection method |
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| CN112858281A (en) * | 2021-03-02 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Integrated rapid detection tube and method for rapidly detecting purity of sesame oil |
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| CN104297234A (en) * | 2014-10-11 | 2015-01-21 | 北京华益精点生物技术有限公司 | Preparation methods of color developing agent and test paper for testing boric acid and borax |
| CN104483314A (en) * | 2014-12-25 | 2015-04-01 | 桂林中辉科技发展有限公司 | Method for improvingthermal stability of test strips for detecting peroxide value of cooking oil |
| CN106442506A (en) * | 2016-10-14 | 2017-02-22 | 湖南省食品质量监督检验研究院 | Process for rapidly detecting raw-cooked degree of soybean milk |
| CN109258837A (en) * | 2018-09-18 | 2019-01-25 | 安徽阜阳宝鼎粮油有限责任公司 | A kind of formula of drinkable sesame oil |
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| Publication number | Publication date |
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| CN111610182A (en) | 2020-09-01 |
| CN113466226A (en) | 2021-10-01 |
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