CN113465298B - South Asia loquat pollen drying and storing method - Google Patents
South Asia loquat pollen drying and storing method Download PDFInfo
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- CN113465298B CN113465298B CN202110806788.5A CN202110806788A CN113465298B CN 113465298 B CN113465298 B CN 113465298B CN 202110806788 A CN202110806788 A CN 202110806788A CN 113465298 B CN113465298 B CN 113465298B
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- 235000009008 Eriobotrya japonica Nutrition 0.000 title claims abstract description 39
- 241001092070 Eriobotrya Species 0.000 title claims abstract description 36
- 238000001035 drying Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 20
- 244000061508 Eriobotrya japonica Species 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 abstract description 11
- 230000007774 longterm Effects 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 1
- 230000035899 viability Effects 0.000 description 7
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000009746 freeze damage Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B3/00—Drying solid materials or objects by processes involving the application of heat
- F26B3/28—Drying solid materials or objects by processes involving the application of heat by radiation, e.g. from the sun
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for drying and storing south Asia loquat pollen, wherein the drying method comprises the following steps: collecting fresh flower anther of Eriobotrya japonica in south Asia, pre-treating for 1-3d at a distance of 8-12cm from an incandescent lamp with power of 20-50W, and drying at 25-35 deg.C for 4-16h to obtain dried pollen. Then the mixture is stored in a sealed way at the temperature of minus 20 ℃. The drying or storage method of the invention can stably maintain the activity of the south Asia loquat pollen under long-term conditions.
Description
Technical Field
The invention relates to the technical field of pollen drying and storing methods.
Background
Loquat (Eriobotrya japonica) is originally produced in China, flowers in autumn, and young fruits are easy to suffer from low-temperature freezing injury after passing through cold winter.
The south Asia loquat (Eriobotrya bentalans) is a wild species in the Eriobotrya, flowers in spring, does not generate freeze injury at all, and has the excellent properties of late mature period, less seeds, strong pest and disease resistance and the like.
The loquat and the south Asia loquat are adopted for interspecific distant hybridization, useful genes and characters of spring flowering and the like of the south Asia loquat are introduced into the loquat, the freezing injury problem of the loquat can be fundamentally solved, the cultivated variety is improved in a large scale, and great theoretical and practical significance is achieved.
However, loquat and south Asia loquat are separated in their flowering period by nearly half a year, and in order to perform crossing, it is necessary to find a suitable pollen drying and storage technique for south Asia loquat.
The prior loquat pollen drying and storing technology is based on the hybridization among different varieties in common loquat seeds, and the flowering phases of the different varieties are very similar, so that the requirements on pollen drying and storing are not very high, and the loquat pollen drying and storing technology only needs to be dried properly and then placed in a room temperature or a refrigerator for short-term storage. The flowering phases of the loquat and the south Asia loquat are nearly half a year apart, the pollen cannot be completely dried by adopting the existing loquat pollen drying and storing method, and the pollen activity is very low after the loquat and the south Asia loquat are stored for half a year at room temperature or in a refrigerator, so that the needs of hybridization work cannot be met.
Disclosure of Invention
The invention aims to provide a drying method capable of stably keeping the activity of the south Asia loquat pollen under a long-term storage period, and also aims to provide a storage method thereof.
The technical scheme of the invention is as follows:
a method for drying south Asia loquat pollen comprises the following steps:
collecting fresh flower anther of Eriobotrya japonica in south Asia, pre-treating for 1-3d at a distance of 8-12cm from an incandescent lamp with power of 20-50W, and drying at 25-35 deg.C for 4-16h to obtain dried pollen.
According to some preferred embodiments of the present invention, in the pretreatment, the power of the incandescent lamp is 40w, the distance between the incandescent lamp and the anther is 10cm, and the treatment time is 2d.
According to some preferred embodiments of the present invention, the temperature of the drying is 30 ℃ and the time of the drying is 4 to 16 hours.
According to some preferred embodiments of the present invention, the temperature of the drying is 30 ℃ and the time of the drying is 8 hours.
The invention further provides a method for storing the south Asia loquat pollen, which comprises the step of hermetically storing the south Asia loquat pollen dried by the drying method.
According to some preferred embodiments of the invention, the temperature of the sealed storage is between-20 ℃ and room temperature.
Further preferably, the temperature of the sealed storage is-20 ℃.
According to some preferred embodiments of the invention, the period of storage is from 0 to 210 days.
According to some preferred embodiments of the drying or storage method of the present invention, the fresh south asian loquat flower anther is obtained from flower buds of south asian loquat flower collected under clear day conditions in the middle of 4 months.
According to some preferred embodiments of the drying or storage method of the present invention, the flower buds of Eriobotrya japonica in south Asia are collected at 9-11 am.
The invention has the following beneficial effects: compared with the pollen drying and storing method in the prior art, the invention can obtain the south Asia loquat pollen with stable vitality under the condition of 210 days of storage.
Drawings
FIG. 1 is a schematic representation of pretreatment carried out under an incandescent bulb of loquat in south Asia.
Detailed Description
The present invention is described in detail below with reference to the following embodiments and the attached drawings, but it should be understood that the embodiments and the attached drawings are only used for the illustrative description of the present invention and do not limit the protection scope of the present invention in any way. All reasonable variations and combinations that fall within the spirit of the invention are intended to be within the scope of the invention.
According to the technical scheme of the invention, a specific implementation mode comprises the following steps:
collecting buds containing buds at the periphery of a crown at about 10 am in sunny days in the full bloom stage (4 middle ten days of the month), stripping off anthers, collecting, and placing in a triangular flask or a culture dish;
then pretreating the triangular flask or the culture dish under an incandescent lamp with power of 20-50W, distance between the incandescent lamp and the anther of 8-12cm and treatment time of 1-3d, wherein the process is shown in figure 1.
Drying at 25-35 deg.C for 4-16h, and storing the dried pollen at room temperature-20 deg.C under sealed condition.
The invention further provides comparative examples illustrating the above embodiments as follows:
examples
Collecting buds containing buds at the periphery of a crown at about 10 am in sunny days in the full bloom stage (4 middle ten days of the month), stripping off anthers, collecting, and placing in a triangular flask or a culture dish;
then, the peeled anthers are grouped according to whether incandescent lamp pretreatment is carried out or not and different pretreatment parameters, the specific treatment mode is as shown in figure 1, the power of the incandescent lamp of the pretreatment group is respectively set to be 20w, 40w or 50w, the distance from the anther is respectively set to be 8cm, 10cm or 12cm, the treatment time is respectively set to be 1d, 2d or 3d, and the release effect of the pretreated pollen is tested according to the pollen release amount.
Then drying the pollen in the triangular flask or the culture dish for 4h, 8h, 12h and 16h respectively in an incubator at 30 ℃, detecting the initial activity of the pollen by adopting a TTC method after the drying is finished, sealing, storing the pollen in groups at room temperature (about 20-30 ℃), 5 ℃ and-20 ℃, and detecting the activity of the stored pollen by adopting the TTC method when the pollen is stored for 210 d.
The specific pollen viability detection method comprises the following steps: TTC (2,3,5-triphenyltetrazolium chloride) is added into pollen to be detected to obtain a pollen suspension, then 20 mu l of the pollen suspension is taken for flaking, observation is carried out under an OLYMPUS-BX51 type optical microscope (10 multiplied by 10), if the pollen suspension is dyed in red, viable pollen is obtained, if the pollen suspension is colorless, the total number of the pollen in 5 visual fields and the number of the red pollen are counted, and the pollen viability is calculated through the following formula: pollen viability = number of red pollen/total number of pollen × 100%.
The pollen release effect under different pretreatments measured by the above procedure is shown in table 1.
TABLE 1 pretreatment effect contrast
Note: more + means more pollen release, -means no pollen release
It can be seen that the pollen release effect was poor without using the incandescent lamp, the pollen release was significantly promoted with the incandescent lamp, and the pollen release effect was best with the 40W incandescent lamp placed 10cm above the anther for 2d irradiation.
The pollen obtained from the pretreatment was irradiated with 40W incandescent lamp placed 10cm above the anther for 2d, and the initial pollen viability at different time and different storage temperature and pollen viability at storage for 210d after drying at 30 ℃ are shown in Table 2.
TABLE 2 comparison of pollen storage Effect under different pollen drying and storage conditions
It can be seen that drying at 30 ℃ for different times and at different storage temperatures has a significant effect on pollen viability, and that pollen viability is maintained optimally after drying at 30 ℃ for 8h followed by storage at-20 ℃.
The above examples are merely preferred embodiments of the present invention, and the scope of the present invention is not limited to the above examples. All technical schemes belonging to the idea of the invention belong to the protection scope of the invention. It should be noted that modifications and embellishments within the scope of the invention may be made by those skilled in the art without departing from the principle of the invention, and such modifications and embellishments should also be considered as within the scope of the invention.
Claims (3)
1. A south Asia loquat pollen storage method after drying is characterized in that: it includes:
collecting fresh flower anther of Eriobotrya japonica in south Asia, placing the flower anther in a position 10cm away from an incandescent lamp with power of 40W for 2d for pretreatment, and drying the flower anther at 30 ℃ for 8h to obtain dry pollen; storing the dried pollen at-20 deg.C under sealed condition.
2. A method of storage after drying according to claim 1, wherein: the storage period is 0-210 days.
3. The storage method after drying according to claim 1 or 2, characterized in that: the fresh south Asia loquat anther is obtained by collecting flower buds of the south Asia loquat flower in 4 th month in clear days, and/or the flower buds of the south Asia loquat flower are collected at 9-11 am.
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| CN202110806788.5A CN113465298B (en) | 2021-07-16 | 2021-07-16 | South Asia loquat pollen drying and storing method |
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