CN113460461A

CN113460461A - Kit for detecting gene mutation and detection method thereof

Info

Publication number: CN113460461A
Application number: CN202110842068.4A
Authority: CN
Inventors: 孙仕亮
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-07-26
Filing date: 2021-07-26
Publication date: 2021-10-01

Abstract

The invention relates to the technical field of a kit for detecting gene mutation, in particular to a kit for detecting gene mutation and a detection method thereof. According to the invention, the placing mechanism is arranged, the sleeve joint plate and the hinge plate can be accommodated and can move towards one side, so that the reagent tubes on the next layer are exposed, the number of the reagent tubes in the accommodating box is increased, the number of the reagent tubes when workers can detect the reagent tubes is increased, and the fault tolerance rate of the workers in the detection process is increased.

Description

Kit for detecting gene mutation and detection method thereof

Technical Field

The invention relates to the technical field of a kit for detecting gene mutation, in particular to a kit for detecting gene mutation and a detection method thereof.

Background

The current gene testing procedures are complicated and highly equipment demanding and currently only applied in laboratories of research institutes, such as the method for medical kits and using the kit disclosed in CN201210169338.0, include an umbilical cord blood storage kit configured in such a way that each process can be performed according to a function-specific kit set for each step by dividing all the processes into the following respective steps: a step of isolating cells, a step of culturing cells, a step of collecting and storing cells, and a step of implanting desired cells into a target site of a body. The cord blood storage kit comprises: a cord blood collection kit, a hematopoietic stem cell separation kit and a hematopoietic stem cell cryopreservation kit. Therefore, the cord blood is stored using the cord blood storage kit by: the method comprises the steps of collecting umbilical cord blood, separating hematopoietic stem cells and performing cryopreservation on the hematopoietic stem cells, wherein the storage amount of a reagent in the reagent kit is fixed in the using process of the reagent kit, so that samples which can be obtained by workers are reduced, the fault tolerance rate in the detection process of the workers is reduced, and certain pressure is caused on the workers, and therefore the reagent kit for detecting the gene mutation and the detection method thereof need to be designed to solve the problems.

Disclosure of Invention

The invention aims to provide a kit for detecting gene mutation and a detection method thereof, and aims to solve the problems that in the use process of the kit provided in the background art, the storage amount of the kit to a reagent is fixed, so that samples which can be obtained by workers are reduced, the fault tolerance rate in the detection process of the workers is reduced, and certain pressure is caused to the workers.

In order to achieve the purpose, the invention provides the following technical scheme: a kit for detecting gene mutation comprises a device main body, wherein the device main body comprises a storage box, movable blocks are arranged on two sides of the top end of the storage box, sealing plates are arranged on one sides, close to each other, of the movable blocks, auxiliary rods are arranged on two sides of the storage box, insertion blocks are arranged on one sides, close to each other, of the auxiliary rods, fixed rods are arranged on the insertion blocks, the two sealing plates can be unfolded and fixed through the fixed rods, a classification plate is arranged in the storage box and divides the inner cavity of the storage box into a storage cavity and a placement cavity, a movable plate is arranged in the storage cavity, two ends of the movable plate are respectively arranged on the classification plate and the side wall of the opposite side of the storage box in a sliding mode, the storage plate is arranged at the top end of the movable plate, and second movable shafts are arranged on two sides of the bottom end of the movable plate and the bottom end of the storage box, the one side that the second loose axle is close to each other all articulates installs and stretches the pole, the one end of stretching the pole all runs through to install and runs through the axle, cooling fan is installed to one side of receiver, it is provided with the placement machine who is used for placing the test agent of waiting to examine to place the intracavity.

As an alternative of the technical scheme of the invention, the close sides of the sealing plates are provided with mounting blocks, one side of one of the mounting blocks is provided with two accommodating grooves which are arranged up and down, the inside of the containing groove is provided with an extension block in a sliding way, one side of the other mounting block close to the extension block is provided with a hollow groove matched with the extension block, the extension blocks can extend into the empty groove, the connecting rods are arranged at the sides, close to each other, of the two extension blocks in the containing groove, the pull rods are arranged at the top ends of the extension blocks, the pulling rod extends to the outer side of the sealing plate, the mounting block at the side of the extension block is provided with a sliding groove, the pulling rod and the connecting rod can move in the sliding groove, and the groove wall of the sliding groove is abutted against the pulling rod and the connecting rod through a damping pad.

As an alternative of the technical scheme of the present invention, the upper and lower sides of the insertion block are both provided with right-angle mounting grooves, extension rods are horizontally inserted into the mounting grooves, the outer surfaces of the extension rods, which are located on the outer side portions of the insertion block, are both sleeved with sleeved springs, the fixing rods are inserted into the other ends of the mounting grooves and are abutted against the extension rods, the portions of the fixing rods, which are located on the outer sides of the mounting grooves, are sleeved with return springs, the two sides of each return spring are respectively fixedly connected with the fixing rods and the insertion block, the top ends of the sealing plates are both provided with accommodating blocks, the top ends of the accommodating blocks are both provided with insertion grooves, and the inside of the accommodating blocks on the two sides of the insertion grooves are both provided with fixing grooves.

As an alternative of the technical scheme of the invention, the sliding blocks are mounted on the sides of the moving plates far away from each other, the sliding grooves are formed in the classifying plates and the side, close to the sliding blocks, of the inner wall of the storage box, the sliding blocks extend into the sliding grooves, and the sliding blocks can slide in the sliding grooves.

As an alternative of the technical scheme of the invention, sliding grooves are formed in the top ends of the moving plates on the two sides of the containing plate, sliding blocks are slidably mounted inside the sliding grooves, a first movable shaft is mounted at the top end of each sliding block, a movable rod is mounted on one side, away from the moving plates, of each first movable shaft, a first movable shaft is mounted at the other end of each movable rod, and the first movable shaft on one side, away from the sliding grooves, of each movable rod is connected with the sealing plate.

As an alternative scheme of the technical scheme of the invention, one side of one of the penetrating shafts is provided with a receiving rod, a buffer spring is fixedly arranged in the receiving rod, the other end of the buffer spring is provided with a sleeve rod, and one side of the sleeve rod, which is far away from the buffer spring, is connected with the other penetrating shaft.

As an alternative of the technical scheme of the invention, the placing mechanism comprises a plurality of first hinge shafts, the plurality of first hinge shafts are respectively arranged on the side wall of the accommodating box on the side far away from the moving plate or on the side wall of the classifying plate on the side far away from the moving plate, one ends of the first hinge shafts are movably provided with hinge plates, one sides of the hinge plates far away from the first hinge shafts are respectively provided with a sheathing plate in a sheathing way, the bottom ends of the sheathing plates are respectively and fixedly provided with an auxiliary block, one side of the bottom ends of the auxiliary blocks is respectively provided with an extending groove, the side wall on the opposite side of the first hinge shaft is correspondingly provided with a second hinge shaft, the other ends of the second hinge shafts are respectively provided with a support rod, and the support rods can extend into the extending grooves.

As an alternative of the technical scheme of the invention, a hanging column is arranged on the upper surface of one side of the sleeving connection plate, which is far away from the hinged plate, a hanging hole is arranged on the side wall corresponding to the side of the first hinged shaft, and the hanging column can be clamped in the hanging hole.

The invention also discloses a detection method of the kit for detecting gene mutation,

firstly, when the mutant gene is collected and detected, the pulling rod is pulled to move to one side far away from the empty groove, so that the extension block is separated from the empty groove, at the moment, the two sealing plates can be unfolded to two sides,

secondly, the extension rod is pulled towards one side far away from the mounting groove to release the conflict of the extension rod to the fixed rod, so that the fixed rod is partially retracted into the mounting groove under the action of a reset spring, the sealing plate on the side where the placing cavity is located is rotated towards the inserting block until the inserting block is clamped into the inserting groove, then the extension rod is loosened, the sleeve spring pushes the extension rod to clamp the fixed rod into the fixing groove, and the unfolded sealing plate is fixed,

thirdly, taking out the reagent and the test tube which are needed to be used from the socket joint plate and the hinged plate, sampling and detecting the mutant gene, lifting the upper layer socket joint plate upwards after the reagent on the socket joint plate and the hinged plate is used, separating the support rod from the extension groove, then accommodating the hinged plate into the socket joint plate to reduce the integral volume of the socket joint plate and the hinged plate, fixing the reduced socket joint plate and the hinged plate on the side wall of the placing cavity through the hanging column and the hanging space, and exposing the socket joint plate and the hinged plate which are originally positioned at one side of the bottom end for use,

the fourth step, accomplish the sample and the back of measuring to the sample, the sample that will retrieve is put into to the space of classification board both sides with reagent, when opening closing plate (112) on the movable plate, the movable rod that sets up on the closing plate upwards mentions the movable plate, make the storage plate rebound that can be used to place the test tube, put into the back to the storage plate with the reagent after the sample, close the closing plate again, make the movable plate rebound, the bottom to the receiver is withdrawed to the reagent of placing on the storage plate, thereby the rethread starts cooling fan and cools off the reagent of placing on the storage plate.

Compared with the prior art, the invention has the beneficial effects that:

1. through being provided with the installation piece, accomodate groove, extension piece, dead slot and pulling pole, the staff moves the pole through removing the pulling to make extension piece card go into to the dead slot in, fix with this closing plate that comes two to close, thereby seal the inside of receiver, thereby prolong the reagent storage life in the inside reagent pipe of receiver.

2. Through being provided with the closing plate, movable plate and movable rod, the staff will upwards remove the movable plate at the in-process movable rod that expandes the closing plate, thereby make things convenient for the test tube that the staff will collect and sample to put into the storage plate, when the staff closes the closing plate again, the storage plate will drive the test tube and move downwards, thereby make cooling fan cool down and cool off the test tube on the storage plate, with this extension place the activity of the sample of collecting in the reagent pipe in the storage plate, thereby the staff detects the accuracy nature of structure after improving.

3. Through being provided with the mechanism of placing, can accomodate and can move about to one side between cover joint board and the articulated slab for the reagent pipe of next layer exposes out, with this figure that increases the inside reagent pipe of receiver, and then increases the reagent pipe figure that the staff can examine time measuring, and increase the staff after and carry out fault-tolerant rate in the testing process.

Drawings

FIG. 1 is a schematic sectional elevation view of the structure of the present invention;

FIG. 2 is a schematic side view of the structure of the present invention;

FIG. 3 is a schematic top sectional view of a movable plate structure according to the present invention;

FIG. 4 is a schematic top view of a hinge plate and sleeve structure according to the present invention;

FIG. 5 is an enlarged view of a portion of the structure A of FIG. 1 according to the present invention;

FIG. 6 is an enlarged view of a portion of the structure shown at B in FIG. 1 according to the present invention;

FIG. 7 is an enlarged view of a portion of the structure of FIG. 1 at C in accordance with the present invention;

fig. 8 is a partially enlarged view of the structure at D in fig. 1 according to the present invention.

In the figure: 100. a device main body; 110. a storage box; 111. a movable block; 112. a sealing plate; 113. an auxiliary lever; 114. inserting a block; 115. an extension rod; 116. sleeving a spring; 117. mounting grooves; 118. fixing the rod; 119. accommodating the block; 120. inserting the groove; 121. fixing grooves; 122. mounting blocks; 123. a receiving groove; 124. a lengthening block; 125. an empty groove; 126. a connecting rod; 127. pulling a rod; 128. moving the plate; 129. a slider; 130. a classification plate; 131. a chute; 132. a storage plate; 133. a slider; 134. a sliding groove; 135. a movable rod; 136. a first movable shaft; 137. a second movable shaft; 138. a stretch rod; 139. penetrating the shaft; 140. a storage rod; 141. a buffer spring; 142. a sleeved rod; 143. a cooling fan; 200. a placement mechanism; 210. a first hinge shaft; 211. a hinge plate; 212. sleeving and connecting plates; 213. an auxiliary block; 214. an extension groove; 215. a second hinge shaft; 216. a support rod.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

Referring to fig. 1-8, an embodiment of the present invention:

a kit for detecting gene mutation comprises a device main body 100, wherein the device main body 100 comprises a storage box 110, movable blocks 111 are arranged on two sides of the top end of the storage box 110, a sealing plate 112 is arranged on one side, close to each other, of each movable block 111, an installation block 122 is arranged on one side, close to each sealing plate 112, of each installation block 122, a storage groove 123 is formed in one side of each installation block 122, the number of the storage grooves 123 is two, the upper portion and the lower portion of each storage groove 123 are provided with an extension block 124 in a sliding mode, a hollow groove 125 is formed in one side, close to each extension block 124, of each installation block 122, the extension block 124 can extend into the hollow groove 125, a connecting rod 126 is arranged on one side, close to each other, of the two extension blocks 124 in the storage groove 123, a pulling rod 127 is arranged on the top end of each extension block 124, the pulling rod 127 extends to the outer side of each sealing plate 112, a sliding groove is formed in the installation block 122 on the side, where the extension block 124 is located, the pulling rod 127 and the connecting rod 126 can move in the sliding groove to drive the extension block 124 to move in the accommodating groove 123 and the empty groove 125, and the groove wall of the sliding groove is abutted against the pulling rod 127 and the connecting rod 126 through a damping pad, so that the pulling rod can be ensured not to slide under the action of an unmanned force, and the sealing effectiveness of the sealing plate 112 can be ensured.

The worker moves the pulling rod 127 so that the extension block 124 is engaged with the empty groove 125, thereby fixing the two closed sealing plates 112, sealing the inside of the storage case 110, and extending the storage life of the reagent in the reagent tube inside the storage case 110.

Further, the two sides of the storage box 110 are both provided with auxiliary rods 113, one side of each auxiliary rod 113 close to each other is provided with an insertion block 114, the upper side and the lower side of each insertion block 114 are both provided with right-angle-shaped installation grooves 117, extension rods 115 are horizontally inserted into the installation grooves 117, the outer surfaces of the extension rods 115 on the outer sides of the insertion blocks 114 are all sleeved with sleeve springs 116, fixing rods 118 are inserted into the other ends of the installation grooves 117 and abut against the extension rods 115, the parts of the fixing rods 118 on the outer sides of the installation grooves 117 are sleeved with return springs, the two sides of the return springs are respectively fixedly connected with the fixing rods and the insertion blocks, the top ends of the sealing plates 112 are all provided with accommodating blocks 119, the top ends of the accommodating blocks 119 are all provided with insertion grooves 120, the inside of the accommodating blocks 119 on the two sides of the insertion grooves 120 are all provided with fixing grooves 121, and a worker can clamp the fixing rods 118 into the fixing grooves 121 after unfolding the sealing plates 112, therefore, the unfolded sealing plate 112 is fixed, so that the reagent tube in the storage box 110 can be conveniently taken out or stored by the staff, and the inconvenience of the staff in the detection process is reduced.

Further, a classification plate 130 is installed inside the storage box 110, the classification plate 130 divides the inner cavity of the storage box 110 into a storage cavity and a placement cavity, two sealing plates 112 are respectively arranged on the upper portions of the storage cavity and the placement cavity, a moving plate 128 is slidably installed inside the storage box 110 inside the classification plate 130, sliders 129 are installed on the sides, away from each other, of the moving plates 128, sliding grooves 131 are respectively formed on the inner walls of the classification plate 130 and the storage box 110, close to the sliders 129, of the sides, close to the sliders 131, the sliders 129 extend into the sliding grooves 131 and can slide in the sliding grooves 131, the storage plate 132 is installed at the top end of the moving plate 128, sliding grooves 134 are respectively formed at the top ends of the moving plates 128 on the two sides of the storage plate 132, sliding blocks 133 are respectively slidably installed inside the sliding grooves 134, a first movable shaft 136 is installed at the top end of the sliding blocks 133, a movable rod 135 is installed on the side, away from the movable rod 136, and a first movable shaft 136 is installed at the other end of the movable rod 135, the first movable shaft 136 of one side of the movable rod 135 far away from the sliding groove 134 is connected with the sealing plate 112, the second movable shaft 137 is installed at both sides of the bottom end of the movable plate 128 and the bottom end inside the storage box 110, the side of the second movable shaft 137 close to each other is hinged with the extending rod 138, one end of the extending rod 138 is provided with the penetrating shaft 139 in a penetrating manner, one side of the penetrating shaft 139 is provided with the storage rod 140, the inside of the storage rod 140 is fixedly provided with the buffer spring 141, the other end of the buffer spring 141 is provided with the sleeve rod 142, one side of the sleeve rod 142 far away from the buffer spring 141 is connected with the penetrating shaft 139, one side of the storage box 110 is provided with the cooling fan 143, the placing mechanism 200 is installed in the placing cavity of one side of the storage box 110 far away from the movable plate 128, the movable rod 135 drives the movable rod 128 to move upwards in the process of unfolding the sealing plate 112 by the worker, and the worker can conveniently place the collected test tube and sample into the storage plate 132, when the staff closed sealing plate 112 again, storage plate 132 will drive the test tube and move down to make cooling fan 143 cool down and cool off the test tube on storage plate 132, place the activity of the sample of collecting in the reagent pipe in storage plate 132 with this extension, and then improve the staff after and carry out the accuracy nature that detects the structure.

Further, the placing mechanism 200 includes a plurality of first hinge shafts 210, the plurality of first hinge shafts 210 are respectively disposed on the side wall of the side of the receiving case 110 far away from the moving plate 128 or on the side wall of the side of the classifying plate 130 far away from the moving plate 128, a hinge plate 211 is movably mounted at one end of the first hinge shaft 210, a socket plate 212 is socket-mounted at one side of the hinge plate 211 far away from the first hinge shaft 210, auxiliary blocks 213 are fixedly mounted at the bottom ends of the socket plate 212, an extending groove 214 is formed at one side of the bottom end of the auxiliary block 213, a second hinge shaft 215 is mounted on the side wall of the inner wall of the receiving case 110 and the classifying plate 130 opposite to the first hinge shaft 210, a support rod 216 is mounted at the other end of the second hinge shaft 215, the support rod 216 can extend into the extending groove 214, a hanging column is disposed on the upper surface of the side of the socket plate 212 far away from the hinge plate 211, a hanging hole is disposed on the side wall corresponding to the first hinge shaft 210, the hanging column can be clamped into the hanging hole. The sleeve joint plate 212 and the hinge plate 211 can be accommodated and can rotate towards one side and then are fixed, so that reagent tubes on the next layer are exposed, the number of the reagent tubes when a worker can detect the reagent tubes is increased, and the fault tolerance rate of the worker in the detection process is increased.

The invention also provides a detection method of the kit for detecting the gene mutation;

in the first step, when the mutant gene is collected and detected, the pulling rod 127 is moved to the side far away from the empty groove 125, so that the extension block 124 is separated from the empty groove 125, at this time, the two sealing plates 112 can be unfolded to both sides,

secondly, pulling the extension rod 115 towards one side far away from the mounting groove 117, releasing the interference of the extension rod 115 to the fixing rod 118, so that the fixing rod 118 is partially retracted into the mounting groove 117 under the action of the return spring, at this time, rotating the sealing plate 112 at the side where the placing cavity is located towards the inserting block 114 until the inserting block 114 is clamped into the inserting groove 120, then loosening the extension rod 115, so that the sleeve spring 116 pushes the extension rod 115 to clamp the fixing rod 118 into the fixing groove 121, thereby fixing the unfolded sealing plate 112,

thirdly, taking out the reagent and the test tube which are needed to be used from the socket plate 212 and the hinge plate 211, sampling and detecting the mutant gene, after the reagent on the socket plate 212 and the hinge plate 211 is used, lifting the upper socket plate 212 upwards to separate the support rod 216 from the extension groove 214, then receiving the hinge plate 211 into the socket plate 212 to reduce the integral volume of the socket plate 212 and the hinge plate 211, fixing the reduced socket plate 212 and the hinge plate 211 on the side wall of the placing cavity through a hanging column and a hanging space, and exposing the socket plate 212 and the hinge plate 211 which are originally positioned at the bottom end side for use,

fourthly, after sampling and detecting the sample, putting the sample and the reagent to be recovered into the space on both sides of the classification plate 130, when the sealing plate 112 on the moving plate 128 is opened, the moving rod 135 arranged on the sealing plate 112 lifts the moving plate 128 upwards, so that the storage plate 132 for placing the test tube moves upwards, putting the sampled reagent into the storage plate 132, closing the sealing plate 112, moving the moving plate 128 downwards, and recovering the reagent on the storage plate 132 to the bottom of the storage box 110, and cooling the reagent on the storage plate 132 by starting the cooling fan 143.

The working principle is as follows: when a worker collects and detects a mutant gene, the worker moves the pulling rod 127 to one side far away from the empty slot, so that the extension block 124 is separated from the empty slot 125, the worker rotates the two sealing plates 112 to two sides, the two sealing plates 112 are unfolded to two sides, the extension rod 115 is pulled to one side far away from the installation slot 117, the collision of the extension rod 115 to the fixing rod 118 is released, the fixing rod 118 is partially retracted into the installation slot 117 under the action of the return spring, at the moment, the sealing plate 112 on the side where the placing cavity is located rotates towards the insertion block 114 until the insertion block 114 is clamped into the insertion slot 120, then the extension rod 115 is released, so that the sleeve spring 116 pushes the extension rod 115 to clamp the fixing rod 118 into the fixing slot 121, so that the unfolded sealing plate 112 is fixed, and then the worker takes out a reagent and a test tube which are required to be used from the sleeve plate 212 and the hinge plate 211, the mutant gene is sampled and detected, after the detected gene quantity is too much, the reagent on the socket plate 212 and the hinge plate 211 is used, a worker can lift the socket plate 212 upwards, so that the supporting rod 216 is separated from the extending groove 214, the hinge plate 211 is accommodated in the socket plate 212 to reduce the overall volume of the socket plate 212 and the hinge plate 211, the reduced socket plate 212 and the hinge plate 211 are rotated towards one side and then fixed on the side wall of the placing cavity through the hanging columns and the hanging holes, and the socket plate 212 and the hinge plate 211 which are originally positioned at the bottom end side are exposed, so that the quantity of the usable reagent and the number of the test tubes are increased.

After the staff finishes sampling and detecting the sample, the staff puts the sample and the reagent that need to be retrieved into the space of classifying plate 130 both sides again, when the staff opened the closing plate 112 on the moving plate 128, movable rod 135 on the closing plate 112 can lift the moving plate 128 upwards, make the storage plate 132 that can be used for placing the test tube upwards move, make things convenient for the staff to put into the reagent after the sample in the storage plate 132, the staff is closing the closing plate 112, make the moving plate 128 remove downwards, so that make the reagent placed on the storage plate 132 withdraw to the bottom of receiver 110, the staff is cooling the reagent placed on the storage plate 132 through starting cooling fan 143, so as to prolong the save time of the reagent on the storage plate 132.

The receiving rod 140 and the sleeve rod 142 installed between the two extending rods 138 are used for forming a buffer during the descending process of the moving plate 128 by the elastic force of the buffer spring 141 during the downward movement of the moving plate 128, so as to reduce the damage of the reagent tube placed in the receiving plate 132 after being impacted.

Claims

1. A kit for gene mutation detection, comprising a device main body (100), characterized in that: the device main body (100) comprises a storage box (110), movable blocks (111) are mounted on two sides of the top end of the storage box (110), sealing plates (112) are mounted on one sides, close to each other, of the movable blocks (111), auxiliary rods (113) are mounted on two sides of the storage box (110), insertion blocks (114) are mounted on one sides, close to each other, of the auxiliary rods (113), fixing rods (118) are arranged on the insertion blocks, the two sealing plates (112) can be unfolded and fixed through the fixing rods (118), a classification plate (130) is mounted inside the storage box (110), the classification plate (130) divides an inner cavity of the storage box (110) into a storage cavity and a placement cavity, a movable plate (128) is arranged in the storage cavity, two ends of the movable plate (128) are respectively arranged on the classification plate (130) and the side wall of the storage box (110) on the opposite sides in a sliding mode, accomodate board (132) is installed on the top of movable plate (128), second loose axle (137) are all installed with the both sides of the inside bottom of receiver (110) to movable plate (128) bottom, one side that second loose axle (137) are close to each other all articulates and installs and stretch pole (138), the one end of stretching pole (138) all runs through to install and runs through axle (139), cooling fan (143) are installed to one side of receiver (110), it is provided with placing mechanism (200) that are used for placing the test agent of waiting to examine to detect to place the intracavity.

2. The kit for gene mutation detection according to claim 1, wherein: the sealing plate is characterized in that mounting blocks (122) are mounted on one side, close to each other, of the sealing plate (112), one side of each mounting block (122) is provided with a containing groove (123), the number of the containing grooves (123) is two, extending blocks (124) are arranged in the containing grooves (123) in a sliding mode, one side, close to each extending block (124), of each mounting block (122) is provided with a hollow groove (125) matched with each extending block (124), each extending block (124) can extend into each hollow groove (125), two extending blocks (124) in each containing groove (123) are mounted on one side, close to each other, of each connecting rod (126), a pulling rod (127) is mounted at the top end of each extending block (124), each pulling rod (127) extends to the outer side of the sealing plate (112), and sliding grooves are formed in the mounting blocks (122) on the side, where the extending blocks (124) are located, the pulling rod (127) and the connecting rod (126) can move in the sliding groove, and the groove wall of the sliding groove is abutted against the pulling rod (127) and the connecting rod (126) through a damping pad.

3. The kit for gene mutation detection according to claim 1, wherein: the upper and lower sides of the insertion block (114) are both provided with right-angle mounting grooves (117), an extension rod (115) is horizontally inserted into the mounting groove (117), a sleeve spring (116) is sleeved on the outer surface of the extension rod (115) positioned on the outer side part of the insertion block (114), the fixing rod (118) is inserted into the other end of the mounting groove (117) and is abutted against the extension rod (115), a return spring is sleeved on the part of the fixing rod (118) positioned outside the mounting groove (117), two sides of the reset spring are respectively fixedly connected with the fixed rod and the inserting block, the top end of the sealing plate (112) is provided with an accommodating block (119), the top end of the containing block (119) is provided with an insertion groove (120), and the containing block (119) at two sides of the insertion groove (120) is internally provided with a fixing groove (121).

4. The kit for gene mutation detection according to claim 1, wherein: the movable plate (128) is far away from each other and is provided with a sliding block (129), the classifying plate (130) and one side of the inner wall of the storage box (110) close to the sliding block (129) are provided with a sliding groove (131), and the sliding block (129) extends to the inside of the sliding groove (131) and can slide in the sliding groove.

5. The kit for gene mutation detection according to claim 1, wherein: sliding tray (134) have all been seted up on movable plate (128) top of accomodating board (132) both sides, sliding tray (134) inside all slidable mounting has sliding block (133), first loose axle (136) are installed on the top of sliding block (133), movable rod (135) are installed to one side that movable plate (128) were kept away from in first loose axle (136), and first loose axle (136) are installed to the other end of movable rod (135), first loose axle (136) that sliding tray (134) one side was kept away from in movable rod (135) are connected with closing plate (112).

6. The kit for gene mutation detection according to claim 1, wherein: one side of one of the penetrating shaft (139) is provided with a containing rod (140), a buffer spring (141) is fixedly arranged inside the containing rod (140), the other end of the buffer spring (141) is provided with a sleeving rod (142), and one side, far away from the buffer spring (141), of the sleeving rod (142) is connected with the other penetrating shaft (139).

7. The kit for gene mutation detection according to claim 1, wherein: the placing mechanism (200) comprises a plurality of first articulated shafts (210), the plurality of first articulated shafts (210) are respectively arranged on the side wall of the containing box (110) far away from the moving plate (128) or the side wall of the classifying plate (130) far away from the moving plate (128), one end of the first hinge shaft (210) is movably provided with a hinge plate (211), one side of the hinge plate (211) far away from the first hinge shaft (210) is sleeved with a sleeved plate (212), the bottom ends of the sleeving and connecting plates (212) are fixedly provided with auxiliary blocks (213), one side of the bottom ends of the auxiliary blocks (213) is provided with an extending groove (214), a second hinge shaft (215) is correspondingly installed on the side wall of the opposite side of the first hinge shaft (210), the other end of the second hinge shaft (215) is provided with a support rod (216), and the support rod (216) can extend to the inside of the extension groove (214).

8. The kit for gene mutation detection according to claim 7, wherein: the upper surface of one side, away from the hinged plate (211), of the sleeved plate (212) is provided with a hanging column, a hanging hole is formed in the side wall, corresponding to the side where the first hinged shaft (210) is located, of the side wall, and the hanging column can be clamped into the hanging hole.

9. The method for detecting a kit for gene mutation detection according to any one of claims 1 to 8, wherein;

firstly, when the mutant gene is collected and detected, the pulling rod (127) is pulled to move to one side far away from the empty groove (125) so that the extension block (124) is separated from the empty groove (125), at the moment, the two sealing plates (112) can be unfolded to two sides,

secondly, pulling the extension rod (115) towards one side far away from the mounting groove (117) to release the interference of the extension rod (115) to the fixing rod (118), so that the fixing rod (118) is partially retracted into the mounting groove (117) under the action of a return spring, at the moment, the sealing plate (112) on the side where the placing cavity is located rotates towards the inserting block (114) until the inserting block (114) is clamped into the inserting groove (120), then loosening the extension rod (115) to enable the sleeve spring (116) to push the extension rod (115) to clamp the fixing rod (118) into the fixing groove (121), and further fixing the unfolded sealing plate (112),

thirdly, taking out the reagent and the test tube which are needed to be used from the socket plate (212) and the hinge plate (211), sampling and detecting the mutant gene, lifting the upper socket plate (212) upwards after the reagent on the socket plate (212) and the hinge plate (211) is used, separating the support rod (216) from the extension groove (214), then receiving the hinge plate (211) into the socket plate (212) to reduce the integral volume of the socket plate (212) and the hinge plate (211), fixing the socket plate (212) and the hinge plate (211) which are reduced on the side wall of the placing cavity through hanging columns and hanging holes, and exposing the socket plate (212) and the hinge plate (211) which are originally positioned on the bottom end side for use,

and fourthly, after sampling and detecting the samples, putting the samples and the reagents which need to be recovered into spaces at two sides of the classification plate (130), when opening the sealing plate (112) on the moving plate (128), lifting the moving plate (128) upwards by a movable rod (135) arranged on the sealing plate (112), enabling the containing plate (132) which can be used for containing the test tube to move upwards, putting the sampled reagents into the containing plate (132), closing the sealing plate (112), enabling the moving plate (128) to move downwards until the reagents placed on the containing plate (132) are collected to the bottom end of the containing box (110), and then starting the cooling fan (143) to cool the reagents placed on the containing plate (132).