CN113441451A

CN113441451A - Membrane washing device for immunoblotting

Info

Publication number: CN113441451A
Application number: CN202110572408.6A
Authority: CN
Inventors: 林泳
Original assignee: Individual
Current assignee: Beijing Sino Factor Technology Co ltd
Priority date: 2021-05-25
Filing date: 2021-05-25
Publication date: 2021-09-28
Anticipated expiration: 2041-05-25
Also published as: CN113441451B

Abstract

The invention relates to the technical field of medical detection and discloses a membrane washing device for an immunoblotting method. This a wash membrane device for immunoblotting method, change the membrane and directly place in the layer board, each changes the membrane and separates each other, effectively avoided changeing the membrane and removed and pile up, guarantee to wash and seal the effect, secondly, act on changeing the membrane through swaing and flowing together, simultaneously, flow and see through the layer board and wash the membrane from the bottom, guarantee that the liquid stream is abundant with changeing the membrane contact, on the other hand, through washing the chamber, the pressure storage chamber, the container cavity, the circulation that manifold and backward flow chamber are constituteed washes the structure, the use of driving pump and connecting line has been reduced, realize swaing through the efflux simultaneously, simplify the equipment structure, reduce the cost of whole equipment, the efficiency of washing has been guaranteed.

Description

Membrane washing device for immunoblotting

Technical Field

The invention relates to the technical field of medical detection, in particular to a membrane washing device for an immunoblotting method.

Background

Immunoblotting is commonly used for identifying a certain protein, and can perform qualitative and semi-quantitative analysis on the protein, and is a conventional detection means for detecting diseases in the field of medical detection, wherein in the stage of immunolocalization, after primary antibody incubation and secondary antibody incubation, the primary antibody and the secondary antibody need to be washed to wash out nonspecific binding except for target antigen and antibody, so as to ensure detection accuracy, and in order to avoid direct binding of the antibody and a membrane, a blocking solution needs to be used for filling a point which is not bound with the target protein before incubation.

The membrane is sealed and washed by placing the membrane in a membrane washing dish, placing the membrane on a shaking table to slightly oscillate, and because the membrane in the membrane washing dish is not fixed and the membrane is similar to colloid and water in density, the membrane is easy to suspend, the membrane can be stacked together in the shaking process, the washing effect is reduced, and the final detection precision is affected.

Secondly, the cleaning liquid and the sealing liquid can be recycled, and the existing automatic device usually needs to be provided with a container and a recovery container for each cleaning liquid and a driving pump corresponding to each container, so that the whole structure is too complex, and the equipment cost is increased.

Disclosure of Invention

Aiming at the defects of the background technology, the invention provides the technical scheme of the membrane washing device for the immunoblotting method, which has the advantages of good washing effect, high efficiency, simple structure and the like, and solves the problems in the background technology.

The invention provides the following technical scheme: the utility model provides a wash membrane device for immunoblotting method, includes the casing, the inside of casing from top to bottom is equipped with in proper order washes chamber, pressure-accumulating chamber and manifold, pressure-accumulating chamber passes through manifold and pump drive intercommunication, the pump drive liquid is taken out in the manifold and is held the pressure in the pressure-accumulating chamber and maintain pressure-accumulating chamber, the container intracavity is equipped with a plurality of containers, the lower extreme of container is respectively through second solenoid valve intercommunication manifold, the upper end of container through first solenoid valve with wash the chamber intercommunication, it is equipped with the efflux board to wash between chamber and the pressure-accumulating chamber, it is connected with the layer board through connecting the rope to penetrate the board, the middle part of connecting the rope connection layer board, one side of penetrating the board is equipped with the control, the control makes fluid regard as the connecting rope to be bound, and the water pressure of both sides is opposite change, the manifold intercommunication has the trachea.

Preferably, be equipped with the backward flow chamber between pressure accumulation chamber and the container chamber, arbitrary first solenoid valve all communicates with the bottom in backward flow chamber, the overflow pipe is higher than the highest position of layer board with the intercommunication position in washing the chamber, communicate through the third solenoid valve between pressure accumulation chamber and the control.

Preferably, the efflux board is inlayed and is had a plurality of mesh boards, and what every mesh board corresponds is equipped with and connects rope and layer board, the control is reciprocating motion's shielding plate, be equipped with the hole of sheltering from that does not correspond completely with the mesh of mesh board on the control, the control is when reciprocating motion to the range of sheltering from of the mesh of connecting rope one side grow gradually, and the range of sheltering from of opposite side diminishes gradually.

Preferably, the supporting plate is provided with a pressure reduction hole with a small diameter of the mesh hole on the diameter arm jet flow plate.

Preferably, the layer board includes the horizontal plate, the horizontal plate is equipped with the pterygoid lamina for the both sides of connecting the rope, the top fixedly connected with of horizontal plate has the guide plate towards the horizontal plate, the both ends of horizontal plate are equipped with the limiting plate.

The invention has the following beneficial effects:

1. this a wash membrane device for immunoblotting method, change the membrane and directly place in the layer board, each changes the membrane and separates each other, effectively avoided changeing the membrane and removed and pile up, guarantee to wash and seal the effect, secondly, act on changeing the membrane through rocking and flowing in common, simultaneously, flow and see through the layer board and wash the membrane from the bottom, guarantee that the liquid stream is abundant with changeing the membrane contact, further improve and change membrane cleaning performance, and is still, because liquid is in faster flow, can take away the non-target antibody that is washed away fast, further improvement flushing performance and washing performance.

2. This a wash membrane device for immunoblotting method through washing the circulation washing structure that chamber, pressure storage chamber, container chamber, manifold and backward flow chamber are constituteed, on the one hand, has reduced the use of driving pump and connecting tube, simultaneously, sways and realizes through the efflux, further retrencies equipment structure, reduces the cost of whole equipment, and on the other hand washes and retrieves and goes on in step, and the pipeline is shorter, has guaranteed washing efficiency.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the position relationship of the jet plate and the control member of the present invention;

FIG. 3 is a schematic view of the structure of the jet plate and the control member of the present invention;

fig. 4 is a schematic structural view of the pallet of the present invention.

In the figure: 1. a housing; 2. flushing the cavity; 3. a pressure storage chamber; 4. a container cavity; 5. a manifold; 6. a container; 7. a first solenoid valve; 8. a second solenoid valve; 9. driving the pump; 10. a jet plate; 11. a connecting rope; 12. a support plate; 121. a horizontal plate; 122. a wing plate; 123. a baffle; 124. a limiting plate; 13. an air tube; 14. an overflow pipe; 15. a control member; 16. a reflux cavity.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, a membrane washing device for immunoblotting comprises a housing 1, a washing chamber 2, a pressure accumulation chamber 3 and a flow collection chamber 5 are sequentially arranged in the housing 1 from top to bottom, the pressure accumulation chamber 3 is communicated with a driving pump 9 through the flow collection chamber 5, the driving pump 9 pumps liquid in the flow collection chamber 5 into the pressure accumulation chamber 3 and maintains the pressure in the pressure accumulation chamber 3, the driving pump 9 is arranged in the middle of a container chamber 4, a water inlet pipe of the driving pump 9 extends to the bottom of the flow collection chamber 5 to ensure that the liquid in the flow collection chamber 5 can be sucked, a plurality of containers 6 are arranged in the container chamber 4, the lower ends of the containers 6 are respectively communicated with the flow collection chamber 5 through a second electromagnetic valve 8, the upper ends of the containers 6 are communicated with the washing chamber 2 through a first electromagnetic valve 7, a jet flow plate 10 is arranged between the washing chamber 2 and the pressure accumulation chamber 3, the liquid in the flow collection chamber 6 flows into the flow collection chamber 5, the driving pump 9 pumps the liquid into the pressure accumulation chamber 3 and maintains a certain pressure, the jet flow plate 10 is connected with a supporting plate 12 through a connecting rope 11, the connecting rope 11 is connected with the middle part of the supporting plate 12, one side of the jet flow plate 10 is provided with a control part 15, the control part 15 enables fluid to be bounded by the connecting rope 11, the water pressures on two sides are opposite, namely when the water pressure on one side of the connecting rope 11 is increased, the other side of the connecting rope 11 is decreased, the supporting plate 12 is rocked and swung under the water pressure, the flushing effect is improved, the collecting cavity 5 is communicated with an air pipe 13, the air pipe 13 is higher than the container 6, high pressure and negative pressure are prevented from being formed in the collecting cavity 5, and the natural flow of the liquid is ensured. The density of the blade 12 is less than or similar to the density of the cleaning fluid so that it floats under the pressure of the jet.

Wherein, be equipped with backward flow chamber 16 between pressure accumulation chamber 3 and the container chamber 4, arbitrary first solenoid valve 7 all communicates with the bottom in backward flow chamber 16, the intercommunication position of overflow pipe 14 and washing chamber 2 is higher than the highest position of layer board 12, liquid flows into through pressure accumulation chamber 3 and washes in the chamber 2, wash and flow back and collect in backward flow chamber 16 after the tunica adventitia, the first solenoid valve 7 of the container 6 that corresponds is opened, liquid backward flow indicates in the container 6, communicate through the third solenoid valve between pressure accumulation chamber 3 and the control 15, after once washing is accomplished, open the third flushometer, let the liquid that is less than overflow pipe 14 flow back to in backward flow chamber 16.

Referring to fig. 2 and 3, a plurality of mesh plates are embedded in the jet plate 10, liquid passes through the mesh plates under pressure to form jet flow, each mesh plate is correspondingly provided with a connecting rope 11 and a supporting plate 12, the connecting rope 11 is connected to the central line position of the mesh plates and can be connected through a buckle, the control member 15 is a reciprocating shielding plate and can be driven by an eccentric wheel, an air cylinder or a linear motor, the control member 15 is provided with shielding holes which are not completely corresponding to the meshes of the mesh plates, the shielding range of the control member 15 on one side of the connecting rope 11 is gradually enlarged and the shielding range on the other side of the connecting rope 11 is gradually reduced during reciprocating motion, and thus, impact force on two sides of the bottom of the supporting plate 12 is continuously changed, so that the supporting plate is continuously swung.

Wherein, be equipped with the little pressure reducing hole of diameter arm efflux board 10 upper mesh hole diameter on the layer board 12, through the higher efflux of velocity of flow on the efflux board 10, guarantee the drive to layer board 12, the pressure reducing hole of layer board 12 reduces the impact force of rivers, avoids forming great impact force to the rotating-film.

Please refer to fig. 4, wherein, the supporting plate 12 includes a horizontal plate 121, two sides of the horizontal plate 121 relative to the connecting rope 11 are provided with wing plates 122, the wing plates 122 are used for making two sides move towards the middle, so as to prevent the supporting plate 12 from deviating to one side, the top of the horizontal plate 121 is fixedly connected with a guide plate 123 facing the horizontal plate 121, water flow penetrates through the pressure-reducing hole and then is guided by the guide plate 123 to turn the film, so as to improve the washing effect, two ends of the horizontal plate 121 are provided with limiting plates 124, the jet flow can flow out from two ends of the horizontal plate 121, and the limiting plates 124 shield the film, so as to prevent the film from being washed out.

The working principle and the working process of the invention are as follows:

filling corresponding liquid in each container 6 according to the flushing step in sequence, opening a second electromagnetic valve 8 and a first electromagnetic valve 7, enabling the liquid to flow into a collecting cavity 5, then opening a driving pump 9, pumping the liquid into a pressure accumulation cavity 3, forming jet flow through a jet flow plate 10 under the pressure provided by the driving pump 9 after the pressure accumulation cavity 3 is full, opening a control part 15 after the liquid is higher than a supporting plate 12 to enable the water flow on two sides of a connecting rope 11 to change, enabling the supporting plate 12 to start to swing under the action of the changed jet flow, then enabling the liquid flow through the supporting plate 12 to bring the flushing effect for the rotating film, enabling the liquid to flow back into a return cavity 16 through an overflow pipe 14, then enabling the liquid to flow back into the container 6 through the first electromagnetic valve 7, firstly closing the second electromagnetic valve 8 and opening a third electromagnetic valve to enable the driving pump 9 to continue to work, and stopping after the liquid in the collecting cavity 5 is completely pumped, the liquid in the flushing chamber 2 flows completely back into the return chamber 16 and back into the corresponding container 6, starting the next round of cleaning.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A membrane washing device for immunoblotting, comprising a housing (1), characterized in that: the inside of casing (1) from top to bottom is equipped with wash chamber (2), pressure accumulation chamber (3) and collecting chamber (5) in proper order, pressure accumulation chamber (3) are through manifold (5) and driving pump (9) intercommunication, liquid is taken out in pressure accumulation chamber (3) and is maintained the pressure in pressure accumulation chamber (3) in driving pump (9) with manifold (5), be equipped with a plurality of containers (6) in container chamber (4), the lower extreme of container (6) communicates manifold (5) through second solenoid valve (8) respectively, the upper end of container (6) is through first solenoid valve (7) and wash chamber (2) intercommunication, be equipped with between wash chamber (2) and pressure accumulation chamber (3) and penetrate board (10), it is connected with layer board (12) to penetrate board (10) through connecting rope (11), connecting rope (11) connects the middle part of layer board (12), one side of the jet flow plate (10) is provided with a control piece (15), the control piece (15) enables fluid to be bounded by a connecting rope (11), the water pressures on the two sides are changed in an opposite mode, and the collecting cavity (5) is communicated with an air pipe (13).

2. The membrane washing device for immunoblotting according to claim 1, wherein: pressure storage chamber (3) and container chamber (4) between be equipped with backward flow chamber (16), arbitrary first solenoid valve (7) all communicate with the bottom in backward flow chamber (16), overflow pipe (14) and the communication position that washes chamber (2) are higher than the highest position of layer board (12), through the third solenoid valve intercommunication between pressure storage chamber (3) and control (15).

3. The membrane washing device for immunoblotting according to claim 1, wherein: jet flow board (10) are inlayed and are had a plurality of mesh boards, and what every mesh board corresponds is equipped with and connects rope (11) and layer board (12), control (15) are reciprocating motion's shielding plate, be equipped with the hole that shelters from that does not correspond completely with the mesh of mesh board on control (15), control (15) are when reciprocating motion to the range of sheltering from of the mesh of connecting rope (11) one side grow gradually, and the range of sheltering from of opposite side diminishes gradually.

4. The membrane washing device for immunoblotting according to claim 1, wherein: the supporting plate (12) is provided with a pressure reduction hole with a diameter arm jet plate (10) and a small diameter of the upper mesh hole.

5. The membrane washing device for immunoblotting according to claim 1, wherein: layer board (12) are including horizontal plate (121), horizontal plate (121) are equipped with pterygoid lamina (122) for the both sides of connecting rope (11), the top fixedly connected with of horizontal plate (121) is guide plate (123) towards horizontal plate (121), the both ends of horizontal plate (121) are equipped with limiting plate (124).