CN113439743B

CN113439743B - Growth regulator composition containing sodium salicylate and hemiphyllin

Info

Publication number: CN113439743B
Application number: CN202110711097.7A
Authority: CN
Inventors: 刘润强; 王翠玲; 韩奥辉; 李冬植; 徐莉; 周锋; 张佩; 李景冲; 张百重
Original assignee: Henan Institute of Science and Technology
Current assignee: Henan Institute of Science and Technology
Priority date: 2021-06-25
Filing date: 2021-06-25
Publication date: 2022-04-15
Anticipated expiration: 2041-06-25
Also published as: CN113439743A

Abstract

The invention relates to the technical field of plant growth regulators, in particular to a growth regulator composition containing sodium salicylate and hemiphyllin. A growth regulator composition containing sodium salicylate and hemiphyllin comprises sodium salicylate and hemiphyllin as effective components. The hemiphyllin and the sodium salicylate in the composition can obviously play a synergistic effect role, have obvious action effect, can obviously improve the growth of crops such as wheat, rice, corn, soybean, peanut and the like under the stress environment by lasting action, and can effectively relieve the damage of salt stress to the crops such as wheat, rice, corn, soybean, peanut and the like in the growth and development process.

Description

Growth regulator composition containing sodium salicylate and hemiphyllin

Technical Field

The invention relates to the technical field of plant growth regulators, in particular to a growth regulator composition containing sodium salicylate and hemiphyllin.

Background

In the world, soil salinization is an important factor threatening food safety, and the utilization of land of about 8.31 hundred million hectares is restricted. Salt stress has great influence on the growth and development of plants, and can cause the withering and even death of the whole plants when the salt stress is serious, and the stress environment has great limitation on the healthy growth of the plants. The salt stress is most obviously influenced by sodium salt stress, and the damage area of the salt stress accounts for 45 percent of the total area of the salinized soil. The first significant influence of salt stress on plants is that the growth rate of the plants is forced to be reduced, so that the plant height, the root length, the leaf area, the total number of underground main roots and fibrous roots, the root thickness and the stem thickness of the plants are limited, the fresh weight and the dry weight of plant tissues are greatly reduced in the final morphological characteristics, and particularly, the reduction amplitude before and after the dry and fresh weight of the overground part leaves is very obvious. During the plant physiology, excessive salt in the tissue directly interferes with plant metabolism, thereby causing a large accumulation of osmotic substances in the plant, and the accumulation of these substances can reduce water loss to improve water absorption capacity. Salt stress also causes a large accumulation of Reactive Oxygen Species (ROS) in plants, thereby altering the antioxidant capacity of cells, ultimately causing oxidative damage. The ROS content in the antioxidant defense system plays a very important role in plant growth and development.

At present, two main solutions are provided for solving the salt stress of soil salinization on wheat: one is a transgenic technology, and by cloning and analyzing genes related to salt tolerance of wheat and adopting the transgenic technology to obtain heterologous expression in wheat plants, a new salt-tolerant wheat variety is cultivated; another method is to apply plant growth regulator to regulate the physiological activity and related substance content in plant body to enhance the high salt tolerance of wheat, so as to generate resistance to the damage caused by salt stress. Researches prove that the plant growth regulator can enhance the salt tolerance of plants, and researches of Egamberdieva D. Fulvina et al found that ethylene was associated with main root elongation, lateral root formation, adventitious root formation and formation of aerated tissue formation, thereby enhancing stress resistance and adaptability of seedlings.

Sodium salicylate, also known as sodium ortho-hydroxybenzoate, is a derivative of Salicylic Acid (SA) and has similar biological activity, and the common method is foliar spraying with a concentration of 0.05-0.1%. Since the end of the seventies of the twentieth century, SA was found to have a protective effect on plants infected with pathogens, and a great deal of research was conducted on SA-induced Systemic Acquired Resistance (SAR) of plants, which proved that SA as a signal molecule plays a role in inducing SAR formationHas important function. SA not only induces plants to resist biotic stress, but also can induce the plants to generate certain resistance to abiotic stress such as high temperature, low temperature, drought, salt damage and the like. Research results show that SA can induce the stress resistance of plants by regulating the change of the active oxygen level and changing the content of the antioxidant and the activity of enzyme systems thereof. Original Yongbing et al found that 0.25-10.0mmol/L SA can obviously improve H in apple leaves ₂0₂The reason for this is that SA not only inhibits CAT activity but also inhibits H ₂0₂The decomposition of (A) causes a large accumulation in the apple leaf tissue. But also probably due to increased SOD activity by SA or activation of some other related enzymes.

Hemiphyllin, also known as N-acetylthioproline. The derivative is a medical intermediate with the effect of protecting myocardial ischemia, and is also an organic amino acid derivative. Research reports that the hemiphyllin can regulate the growth and development of crops, promote the germination of seeds, and improve the fruiting rate and the quality and the yield of fruits. Debashish et al showed that the application of a certain amount of hemiphyllin to apricots had a significant effect on the diameter, weight, size and yield of their fruits, all of which were significantly increased by 15.3%, 25.9%, 27.1% and 40.0% respectively as compared to the controls. Katrina et al found that spraying of hemiphyllin on apples of different varieties can promote the development of flower buds and fruits to a great extent, and significantly increase the fruit setting number, fruit quality and fruit weight.

At present, no literature data related to the reduction of plant salt stress influence by the mixed use of sodium salicylate and hemin on wheat is found.

The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Disclosure of Invention

The invention aims to provide a growth regulator composition containing sodium salicylate and semifolin, wherein the sodium salicylate and the semifolin which are effective components have synergistic effect, so that the stress resistance of wheat can be obviously improved, and the growth regulator composition provides help for the planting of wheat in saline-alkali soil.

In order to achieve the purpose, the invention provides the following technical scheme:

a growth regulator composition containing sodium salicylate and hemiphyllin comprises sodium salicylate and hemiphyllin as effective components.

Preferably, the concentration of the salicylic acid is 0.05mg/L, and the concentration of the hemiphyllin is 25 mg/L.

The invention also provides application of the growth regulator composition in improving the stress resistance of saline-alkali soil crops, and preferably, the crops are wheat.

Compared with the prior art, the invention has the following beneficial effects:

the hemiphyllin and the sodium salicylate in the composition can remarkably play a synergistic effect, have remarkable action effect, can remarkably improve the growth of crops such as wheat, rice, corn, soybean, peanut, cotton, tomato, cucumber, orange, apple and the like under stress environment by lasting action, and can effectively relieve the damage of salt stress to the crops such as wheat, rice, corn, soybean, peanut, cotton, tomato, cucumber, orange, apple and the like in the growth and development process.

Drawings

FIG. 1 shows the measurement results of the content of Malondialdehyde (MDA) to the degree of oxidative stress in each treatment group;

FIG. 2 shows hydrogen peroxide (H) for each treatment group₂O₂) The result of content measurement;

FIG. 3 shows superoxide anions (O) for each treatment group^2-) The result of content measurement;

FIG. 4 shows the measurement results of Peroxidase (POD) activity of each treatment group;

FIG. 5 shows the results of determination of Catalase (CAT) activity in each treatment group;

FIG. 6 shows the results of measurement of superoxide dismutase (SOD) activity in each treatment group.

Detailed Description

In the following, the technical solutions of the present invention will be described clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

1. Materials handling and Experimental methods

1.1 Experimental materials and instruments

Test materials: wheat (Bainong 207), provided by the research center for genetic improvement of wheat of the science and technology institute of Henan province;

the experimental reagents are shown in table 1 below;

TABLE 1 Main test reagents

The experimental equipment is shown in table 2 below;

TABLE 2 Main experimental equipment

1.2 treatment of test materials

Selecting healthy and plump wheat seeds, and adding 10% H₂O₂Soaking in the solution for 10min, repeatedly washing with distilled water for three times to remove H on seed surface₂O₂A solution; after cleaning, putting the seeds in a beaker and carrying out light-resistant treatment for 12 hours for accelerating germination;

placing the seeds after germination acceleration on absorbent paper soaked by saturated calcium sulfate solution in parallel at a distance of 1cm, wherein the side of the fuzz is upward and is 3cm away from the upper edge; clamping the wheat seeds by two pieces of absorbent paper, then rolling up the wheat seeds, and fixing the wheat seeds by using rubber bands;

transplanting the seedlings to a seedling raising pot filled with nutrient solution in a greenhouse to grow under the condition of water culture after one week of seedling raising in the greenhouse under the condition of keeping out of the sun; the transplanted plantlets were randomly divided into 5 groups: blank group, control group, hemiphyllin group, sodium salicylate group and study group;

and 2d, transplanting, and processing as follows:

table 3 contents of different groups of experimental treatments

The salt stress treatment comprises the following specific operations: and 2d of transplanting, adding a salt solution (NaCl: 0.1mol/L) into the nutrient solution to carry out salt stress treatment.

Spraying leaves for 3d and 6d, taking five wheat samples in each group, using the whole wheat sample for determining morphological index and biomass, and taking six seedlings in each group (one biological repeat for every two wheat samples) and storing in a refrigerator at-80 ℃ for later use.

1.3 wheat morphology index determination

Leaves and roots of wheat were rinsed 2-3 times with distilled water and root system scanned using an Epson Expression 12000XL scanner. And adopting WinRHIO Pro 2017 root system picture analysis software to analyze morphological indexes, and simultaneously measuring the plant height of each sample by using a ruler.

And (3) measuring the plant height of the plant: and taking the longest part of the ground parts of the wheat after shearing, measuring the parts one by using a ruler, recording corresponding data, and carrying out three times of parallel measurement to obtain an average value.

And (3) determining the fresh weight of the plant: weighing the corresponding part of the wheat plant on a balance of one ten thousandth, simultaneously recording experimental data, carrying out three-time parallel measurement in each treatment, and finally taking the average value of the measured data to analyze the data.

And (3) measuring the dry weight of the plants: the corresponding parts of the plants weighed to be fresh are respectively put into kraft paper bags, the deactivation of enzymes is carried out for 20min at the temperature of 120 ℃, the plants are dried to be constant weight at the temperature of 70 ℃, each treatment is carried out with three times of parallel measurement, and finally the average value is taken to analyze data.

1.4 assay of Malondialdehyde (MDA)

Preparing an extracting solution: the samples were treated with liquid nitrogen and then ground using a cryo-grinder, after sufficient grinding, using 0.1M PBS (containing 1mM EDTA and 1% PVPP) at pH7.5 as sample: shaking the leaching solution at a ratio of 1:10 to form a uniform slurry, centrifuging at 4 deg.C and 15000g for 20min, and collecting the supernatant;

according to a thiobarbituric acid reaction method, absorbing 400 mu L of sample extracting solution, adding 200 mu L of 1.5% TBA solution into a 2mL centrifuge tube, uniformly mixing, reacting in a water bath kettle in a boiling water bath for 15min, rapidly cooling, and centrifuging; the supernatant was taken to measure the absorbance at wavelengths of 532nm, 600nm and 450 nm.

1.5 Hydrogen peroxide (H)₂O₂) Determination of content

Preparing an extracting solution: grinding 0.1g of the leaf with 0.6mL of acetone pre-cooled at 20 ℃ to obtain homogenate, centrifuging at 4 ℃ for 15min at 10000g, and taking supernatant for later use;

sucking 20 μ L of distilled water, 50 μ L of 400mM sorbitol, 0 μ L of 0.8mM ammonium ferrous sulfate, and 50 μ L of 0.4mM xylenol orange (dissolved in 25mM sulfuric acid), mixing in 2mL centrifuge tube to obtain reaction mixture, adding 30 μ L of extractive solution, mixing, developing in 30 deg.C water bath for 30min, mixing, collecting 200 μ L of test A₅₆₀；

Making a standard curve: respectively collecting 100 μ M hydrogen peroxide (0, 60, 120, 180, 240, 300 μ L), 750 μ L400 mM sorbitol, 750 μ L0.8 mM ferrous ammonium sulfate, 750 μ L0.4mM xylenol orange (dissolved with 25mM sulfuric acid), mixing, developing color in 30 deg.C water bath for 30min, mixing, collecting 200 μ L sample, and measuring A₅₆₀Obtaining a standard curve equation;

and (3) calculating: sample A₅₆₀The standard curve is entered and the desired hydrogen peroxide content is converted.

1.6 superoxide anion (O)^2-) Determination of content

Preparing an extracting solution: grinding and leaching about 0.1g of fresh weight sample with 0.5mL of 50mM PBS (phosphate buffer solution) with pH7.8, centrifuging at 4 ℃ for 20min at 10000g, and taking the supernatant for later use;

the determination steps are as follows:

(1) nitrite generation (addition of sample mixed and then warm bath at 25 ℃ for 1h, shading)

After mixing, shading, and bathing for 1h at 25 ℃ (200 mu L of the current system); the control is that the extracting solution is replaced by PBS with the same volume;

(2) color reaction (Add the following reagents into the preceding reaction tube)

Mixing, shading, and warm bathing at 25 deg.C for 20min (400 μ L total final system, and measuring OD530 with 200 μ L mixing)

The standard curve was prepared as follows:

preparation of reagents: preparing 50 mu M sodium nitrite solution, and sequentially diluting the solution to be 0 mu M, 10 mu M, 20 mu M, 30 mu M, 40 mu M, 50 mu M and other concentration gradients for later use;

the standard curve is 4ml system, the samples are sequentially added according to the following table, mixed uniformly and then bathed for 20min at 25 ℃, and then 200 mu L of sample is taken for determining OD₅₃₀And obtaining a standard curve equation.

Note: wherein the sulfanilic acid is dissolved by distilled water; glacial acetic acid for alpha-naphthylamine: water 3: 1 dissolution of

OD of the sample₅₃₀The standard curve is entered and the desired superoxide anion content is calculated.

1.6 determination of antioxidase Activity

Preparing an extracting solution: the samples were treated with liquid nitrogen and then ground using a cryo-grinder, after sufficient grinding, using 0.1M PBS (containing 1mM EDTA and 1% PVPP) at pH7.5 as sample: shaking the leaching solution 1:10 to form a uniform slurry, centrifuging at 4 ℃ for 20min at 1500g, and taking the supernatant for later use.

1.6.1 Peroxidase (POD) Activity assay

Referring to the guaiacol method, 50mL of 0.1mol/L phosphate buffer (pH6.5) was added with 28. mu.L of guaiacol, and the mixture was dissolved by heatingDissolving, cooling the solution, adding 19 μ L of 30% H₂O₂The solution is evenly mixed to prepare a reaction mixed solution; adding 100 μ L of reaction mixture and 100 μ L of extractive solution into a centrifuge tube, shaking, measuring at wavelength of 475nm for 30s once for 3min, and shaking for 5s before each measurement to obtain reaction curve. The increase in OD per min of 0.01 was 1 enzyme activity unit (U).

The POD enzyme activity (U/mg min, U/g FW min) was calculated according to the reaction curve or Lambert beer's law and the application.

1.6.2 Catalase (CAT) Activity assay

In this experiment, 130. mu.L of 0.1mol/L phosphate buffer (pH7.0) and 30. mu.L of 30% 0.1mol/L H2O2 solution were mixed and shaken in a 2mL centrifuge tube to prepare a reaction solution by the ultraviolet absorption method. Then adding 40 μ L of the extract into the centrifuge tube, replacing the control group with the reaction solution, shaking up, measuring at 240nm wavelength for 30s for one time, measuring for 4min, and shaking for 5s before each measurement to obtain a reaction curve. The OD reduction per minute was 0.01 to 1 enzyme activity unit (U).

Calculating the activity of CAT enzyme (U/mg min, U/g FW min, etc.) according to reaction curve or Lambert beer law and application

1.6.3 determination of superoxide dismutase (SOD)

Referring to NBT photo-reduction method in this experiment, reaction mixture (20. mu.L of 130mM methionine solution, 20. mu.L of 30. mu.M EDTA-Na2 solution, 135. mu.L of 50mM phosphate buffer (pH7.8), 20. mu.L of 0.75mM NBT solution) was prepared in advance in a 2mL centrifuge tube, and then 5. mu.L of the extract was added thereto, shaken well and reacted at 25 ℃ under 4000lx light for 20 min; the control group was replaced with PSB without addition of enzyme solution. The OD560nm values were recorded three times per minute, measured at a wavelength of 560 nm. SOD activity unit 50% inhibition of NBT photochemical reduction was taken as an enzyme activity unit (U).

Statistical analysis and histogram analysis were performed on the data obtained in the above experiment using Microsoft Excel 2010 software, and statistical analysis (ANOVA) was performed on the results using analysis of variance, and significance difference analysis was performed on the data using the duncan new repolarization method using SPSS 17.0 at a significance level of P < 0.05.

2 results and analysis

2.1 measurement results of wheat morphology index

The results are shown in Table 4 below;

TABLE 4 wheat morphology index measurement results

Plant height (cm)

Total root length (cm)

Fresh weight of overground part (g)

Fresh weight of underground (g)

Dry weight of aerial parts (g)

Dry weight of underground part (g)

Blank group³

19.37+1.06bc

943.5+6.9ab

0.701+0.05ab

0.819+0.06a

0.147+0.005a

0.064+0.007ab

Control group³

16.67+0.69c

839.3+69.7b

0.597+0.07b

0.743+0.05a

0.124+0.011a

0.058+0.005b

Hemiphyllin group³

18.40+0.55bc

969.4+106.4ab

0.628+0.05ab

0.751+0.06a

0.152+0.027a

0.073+0.007ab

Sodium salicylate group³

20.63+0.61ab

1195.5+128.7a

0.750+0.04ab

0.947+0.14a

0.140+0.014a

0.083+0.009ab

Research group³

22.33+1.07a

1205.4+17.4a

0.790+0.02a

1.038+0.10a

0.171+0.020a

0.085+0.003a

Blank group⁶

20.96+0.64bc

1660.1+153.7bc

1.091+0.114b

0.91+0.094ab

0.135+0.014ab

0.094+0.003ab

Control group⁶

19.23+0.26c

1498.6+87.8c

0.927+0.020b

0.77+0.054a

0.115+0.007c

0.053+0.007c

Hemiphyllin group⁶

21.90+0.78b

1662.1+50.8bc

1.202+0.074b

0.99+0.030a

0.142+0.008bc

0.083+0.009bc

Sodium salicylate group⁶

23.07+1.18b

2011.3+204.7ab

1.165+0.096b

1.04+0.047a

0.160+0.026ab

0.100+0.018ab

Research group⁶

26.53+0.78a

2236.9+140.4a

1.867+0.172a

1.09+0.079a

0.189+0.03a

0.117+0.012a

Note: the numbers "3" and "6" marked on the capital letters in the treatment groups respectively represent the days after the wheat leaves are sprayed with the medicament; the lower case english letters in the table represent significant differences between the different groups, P < 0.05.

As can be seen from Table 4, after 3d treatment, the physiological properties of wheat under the salt stress environment have obvious promotion effect compared with the blank group and the control group. The plant height, the total root length, the fresh weight of the overground part and the dry weight average of the underground part are obviously different, and the plant heights of the hemiphyllin group, the sodium salicylate group and the research group are respectively increased by 10%, 24% and 36% compared with those of the control group; the total root length is respectively increased by 16%, 42% and 44%; the fresh weight of the overground part is respectively increased by 5 percent, 26 percent and 32 percent; the dry underground weight was increased by 26%, 43%, 47%, respectively. The fresh weight of the underground part and the dry weight of the overground part do not show difference, the sodium salicylate group and the research group have stronger promotion effect on the growth of wheat in the stress environment on the whole, and the hemiphyllin group does not obviously improve the wheat stress environment. The research group has the best growth promoting effect on each growth index of wheat, and the effect is obviously higher than that of single regulator treatment, so that the synergy between the hemiphyllin and the sodium salicylate is shown.

The general trend after treatment 6d and the trend after treatment 3d were substantially the same. However, in the overall comparison, with the continuous action of the regulator, all physiological property indexes of wheat are obviously improved on the whole, and all treatments show obvious differences, after 6d, compared with the data in 3d, the plant height, the total root length, the fresh weight of the overground part and the dry weight of the underground part of the hemiphyllin group are respectively increased by 19%, 71%, 91% and 14%; the sodium salicylate component is respectively increased by 12%, 68%, 55% and 20%; the research groups are respectively increased by 19 percent, 85 percent, 136 percent and 38 percent. Particularly, the underground fresh weight and the overground dry weight of the wheat are different from the prior art to obvious difference, and the underground fresh weight and the overground dry weight of the hemiphyllin group are respectively increased by 32%; the fresh weight of the underground part and the dry weight of the overground part of the sodium salicylate group are respectively increased by 10 percent and 13 percent; the fresh underground weight and the dry underground weight of the study group increased by 5% and 11%, respectively. Comprehensive analysis shows that the effect of the simultaneous treatment of the two regulators on each growth index of the wheat is obviously higher than that of the treatment of a single regulator, and the two regulators show better synergistic effect.

2.2 results of Biomass determination

The results are shown in Table 5 below.

TABLE 5 wheat biomass accumulation assay results

As can be seen from table 5, the projected leaf areas of the sodium salicylate group and the study group at 3d were higher than those of the control group, and the difference between the treatments was very significant. The hemiphyllin group does not have a certain influence on the growth of wheat, but slightly reduces compared with a control group; the leaf projected areas of the sodium salicylate group and the study group were increased by 11% and 22% respectively compared with the control group. The leaf projection area of each treatment group is improved to a certain extent in 6 days compared with that of a control group, the difference among the treatments is very obvious, and the hemiphyllin group, the sodium salicylate group and the research group are respectively improved by 8 percent, 21 percent and 36 percent compared with the control group;

in the aspect of average root diameter, when two growth regulators of a hemiphyllin group and a sodium salicylate group are independently treated under the condition of wheat salt stress at 3d, the effect is not obvious, the average root diameters of the two treated groups are not different, the average root diameters of the hemiphyllin group and the sodium salicylate group are respectively increased by 6.25 percent and 6.27 percent compared with the average root diameter of a control group, and the average root diameters are both lower than the average root diameter of a blank group; the research group has the best effect and obvious difference with other groups, and the effect is respectively increased by 30 percent and 15 percent compared with the control group and the blank group. The general trend at 6d remained approximately consistent, with essentially no change, but the variability between treatments was significant. The effect of the research group is the best, and the sodium salicylate is the second time.

Root surface area measurement data analysis shows that the root surface areas of the three medicament treatment groups in different time periods are higher than those of a control group, and the root surface areas of the hemiphyllin group, the sodium salicylate group and the research group are respectively increased by 14%, 41% and 43% at 3d, but the root surface area of the hemiphyllin group is lower than that of a blank group, wherein the effect of the research group is the best. At 6d, the root surface areas of the hemiphyllin group, the sodium salicylate group and the study group are respectively increased by 0.3%, 39% and 33% relative to the root surface area of the control group, the hemiphyllin group is still lower than the root surface area of the blank group, the promotion effect on wheat is small, the sodium salicylate group has the best effect, and the difference between every two treatments is also obvious.

In conclusion, the effect of the two regulators on the transverse growth of the wheat root system is not obvious when the two regulators are used independently, the growth of the root system can be obviously promoted when the two regulators are mixed, and the effect is obviously higher than that of the single regulator.

2.3 assay of Malondialdehyde (MDA)

The measurement results are shown in FIG. 1.

As can be seen from FIG. 1, the concentration of malondialdehyde in wheat leaves in the hemiphyllin group, the sodium salicylate group and the study group after 3d treatment is lower than that in the blank group and the control group, the difference of each experimental group is obvious, and the hemiphyllin group is respectively reduced by 3% and 7% relative to the blank group and the control group; the sodium salicylate group is respectively reduced by 15 percent and 18 percent; the research groups are respectively reduced by 6% and 10%, which shows that the regulator plays a role in relieving the salt stress suffered by wheat and reducing the damage of leaf tissue cells, thereby reducing the concentration of the malondialdehyde per se. With the continuous action of the regulator, the action effect of single regulator treatment on wheat after 6d of treatment is not changed greatly, and the semifolin group is reduced by 13 percent and 20 percent relative to a blank group and a control group respectively; the sodium salicylate group is respectively reduced by 17 percent and 25 percent; the research groups are respectively reduced by 25 percent and 32 percent, the concentration of malondialdehyde is obviously lower than that of a single regulator treatment group, and the effect of the research group is optimal.

Compared with the effect after 3d, the effect after 6d treatment is that the malondialdehyde concentration of the hemiphyllin group, the sodium salicylate group and the research group is respectively reduced by 17%, 10% and 27%, and the best effect of the research group can be obviously analyzed.

From the above, in the early stage of the wheat leaf spraying treatment, the sodium salicylate treatment effect is optimal, salt stress suffered by wheat is greatly relieved, and damage to leaf histiocytes is reduced, so that the concentration of malondialdehyde is reduced. In the later treatment period, the effect of the regulator is very obvious, and the concentration regulation of the malonaldehyde in a research group is obviously better than that of a single regulator treatment group, which shows that the two regulators play a synergistic effect.

2.4 Hydrogen peroxide (H)₂O₂) Results of content measurement

The measurement results are shown in FIG. 2.

As can be seen from FIG. 2, when the control group and the blank group were compared, a large amount of hydrogen peroxide was produced in the leaf tissue after the wheat was subjected to salt stress. Through the action of the regulator, the hydrogen peroxide concentrations of the hemin group, the sodium salicylate group and the research group are reduced compared with the hydrogen peroxide concentrations of the control group and the blank group after 3d, the difference among the treatments is large, the effect of the research group is optimal, the hydrogen peroxide concentration has obvious promotion effect on the growth of wheat in a stress environment, and the hydrogen peroxide concentrations are reduced by 25% and 22% respectively compared with the hydrogen peroxide concentrations of the control group and the blank group. The hydrogen peroxide content generally decreases gradually by the action of the two regulators 6 d. The hydrogen peroxide concentrations of the hemiphyllin group, the sodium salicylate group and the research group are respectively reduced by 14%, 11% and 15%, and the hemiphyllin group has better effect than the sodium salicylate group along with the continuous action of the two regulators, and the research group has the best effect, which shows that the two regulators have obvious synergistic effect on promoting the growth of wheat under the salt stress, and the salt stress is remarkably relieved.

2.5 superoxide anion (O)^2-) Results of content measurement

The measurement results are shown in FIG. 3;

as can be seen from FIG. 3, the content of superoxide anions in the wheat leaves of the control group is obviously higher than that of the other four treatment groups, which indicates that the high-concentration salt causes great damage to the histiocytes of the wheat leaves, causes a large amount of superoxide anions to be accumulated in the cell sap, and restricts the growth and development of wheat. After the treatment of the two regulators, the content of superoxide anions in the wheat leaves is remarkably reduced relative to a control group and a blank group, the difference between the experimental groups is very obvious, the content of the superoxide anions in the hemiphyllin group and the sodium salicylate group is basically consistent at 3d, the content of the superoxide anions in the research group is respectively reduced by 4% and 1% compared with the control group and the blank group, and the content of the superoxide anions in the research group is lowest and is respectively reduced by 5% and 3% compared with the control group and the blank group. At 6d, with continuous regulation, the initial action effect of the hemiphyllin group and the sodium salicylate group is not obvious, no difference exists between treatments, the hemiphyllin group gradually acts to reduce the accumulation of superoxide anion content, the difference between the two treatment groups is obvious, the regulation effect of the hemiphyllin group is 1.2 times that of the sodium salicylate group, the effect of the research group is still the best, the damage of wheat histiocyte is remarkably repaired, and the large accumulation of superoxide anion in the cell is reduced.

From the above, after the treatment of the hemiphyllin and the sodium salicylate, the accumulation of superoxide anion content is obviously reduced, the damage of wheat tissue cells is repaired, the adjusting effect of the hemiphyllin is slightly higher than that of the sodium salicylate, and the two regulators play a synergistic effect on relieving wheat salt stress.

2.6 measurement results of antioxidase Activity

2.6.1 Peroxidase (POD) Activity measurement results

The measurement results are shown in FIG. 4.

As can be seen from fig. 4, the peroxidase activity of the sodium salicylate group at 3d is the highest, and the difference from the other treated groups is very significant, and is 1.1 times and 1.3 times that of the study group and the hemiphyllin group respectively, and the activities are improved by 36% and 12% relative to those of the control group and the blank group. Meanwhile, the enzyme activity of other treatment groups is higher than that of the control group. At 6d, the wheat of the study group shows the highest peroxidase activity, the enzyme activity of the study group is 1.2 times that of the sodium salicylate group, and the enzyme activity is improved by 101% and 46% relative to the enzyme activity of the control group and the blank group. Overall, the study group treatment was best.

2.6.2 Catalase (CAT) Activity measurement results

The measurement results are shown in FIG. 5.

As can be seen from FIG. 5, in the general trend at 3d, the enzyme activities of the treatments were very different, and the enzyme activities of the hemiphyllin group, the sodium salicylate group and the study group were increased by 4%, 5% and 11% respectively compared with the control group, indicating that the regulator has a certain promotion effect on the growth of wheat under stress. At 6d, enzyme activity differences among the hemiphyllin group, the sodium salicylate group and the research group, the control group and the blank group are very large, and the enzyme activities of the three treatment groups are respectively more than 1.6 times of that of the control group and more than 1.3 times of that of the blank group. Meanwhile, the difference among the treatments is also obvious, and the enzyme activities of the hemiphyllin group, the sodium salicylate group and the research group are respectively increased by 59 percent, 71 percent and 82 percent compared with the control group. From the whole, the adjustment effect is more and more obvious along with the continuous effect of the regulator, and the research group gradually shows that the wheat growth adjustment effect is optimal.

2.6.3 superoxide dismutase (SOD) measurement results

The measurement results are shown in FIG. 6.

As can be seen from FIG. 6, the SOD activity in the control group was the lowest at 3d, while the SOD activity in the other treatment groups was 1.7 times higher than that in the control group, but there was no difference in SOD activity between the treatment groups, and the enzyme activities in the hemiphyllin group, sodium salicylate group and study group were increased by 75%, 76% and 80% respectively, indicating that the regulator still has significant promoting effect on the growth and development of wheat. The enzyme activity difference among the treatments is very obvious at 6d, the superoxide dismutase activity of the control group is still the lowest, the enzyme activity of the sodium salicylate group to wheat is 1.1 times that of the hemiphyllin group, and the enzyme activity of the research group is the highest and is 1.2 times that of the sodium salicylate group. And the enzyme activities of the hemiphyllin group, the sodium salicylate group and the study group are respectively increased by 121%, 138% and 158% compared with the control group. Overall, the treatment with the mixture of hemiphyllin and sodium salicylate gave better results than the individual treatments, with the sodium salicylate being given in the second order.

The result of the antioxidant enzyme activity determination shows that the regulator has slow regulation on wheat in the stress environment in the early stage of leaf spraying treatment, and the activity difference of superoxide dismutase (SOD) is small, but the regulator still has a certain relieving effect on the physiological activity of wheat in the stress environment. In the later treatment period, along with the continuous action of the regulator, the enzyme activities of the treatments show obvious difference, and the regulation of the enzyme activities of the research groups is obviously better than that of the single regulator treatment group; the effect of sodium salicylate in single regulator treatment is better than that of hemiphyllin; shows that the two regulators simultaneously spray the wheat leaves and play a synergistic effect on the regulation of the enzyme activity.

In conclusion, the hemiphyllin and the sodium salicylate can remarkably exert a synergistic effect, have a remarkable action effect, can remarkably improve the growth of wheat under a stress environment under a continuous action, and can effectively relieve the damage of salt stress to the wheat in the growth and development process.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims

1. A growth regulator composition containing sodium salicylate and hemiphyllin is characterized in that the effective components of the growth regulator composition consist of sodium salicylate and hemiphyllin.

2. The growth regulator composition according to claim 1, wherein the concentration of salicylic acid is 0.05mg/L and the concentration of hemiphyllin is 25 mg/L.

3. Use of a growth regulator composition according to any preceding claim for improving stress resistance in saline and alkaline land crops.

4. Use according to claim 3, characterized in that the crops are wheat, rice, maize, soybean and peanut.