CN113397053A - Pig feed additive, pig ration and application thereof - Google Patents
Pig feed additive, pig ration and application thereof Download PDFInfo
- Publication number
- CN113397053A CN113397053A CN202010866042.9A CN202010866042A CN113397053A CN 113397053 A CN113397053 A CN 113397053A CN 202010866042 A CN202010866042 A CN 202010866042A CN 113397053 A CN113397053 A CN 113397053A
- Authority
- CN
- China
- Prior art keywords
- pig
- feed additive
- vitamin
- pig feed
- pigs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003674 animal food additive Substances 0.000 title claims abstract description 40
- 238000000855 fermentation Methods 0.000 claims abstract description 43
- 230000004151 fermentation Effects 0.000 claims abstract description 43
- 241000282887 Suidae Species 0.000 claims abstract description 34
- 239000000835 fiber Substances 0.000 claims abstract description 27
- 239000006041 probiotic Substances 0.000 claims abstract description 24
- 235000018291 probiotics Nutrition 0.000 claims abstract description 24
- 239000002274 desiccant Substances 0.000 claims abstract description 15
- 238000007710 freezing Methods 0.000 claims abstract description 12
- 230000008014 freezing Effects 0.000 claims abstract description 12
- 235000013305 food Nutrition 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- 150000001413 amino acids Chemical class 0.000 claims description 28
- 235000019583 umami taste Nutrition 0.000 claims description 12
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 11
- 235000010755 mineral Nutrition 0.000 claims description 11
- 239000011707 mineral Substances 0.000 claims description 11
- 239000011573 trace mineral Substances 0.000 claims description 11
- 235000013619 trace mineral Nutrition 0.000 claims description 11
- 238000004108 freeze drying Methods 0.000 claims description 8
- 102000008186 Collagen Human genes 0.000 claims description 7
- 108010035532 Collagen Proteins 0.000 claims description 7
- 229920001436 collagen Polymers 0.000 claims description 7
- 238000000338 in vitro Methods 0.000 claims description 7
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 6
- 244000052616 bacterial pathogen Species 0.000 claims description 4
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 4
- 238000012165 high-throughput sequencing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 235000002639 sodium chloride Nutrition 0.000 claims description 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 3
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 3
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004151 Calcium iodate Substances 0.000 claims description 3
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 claims description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 3
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 3
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 3
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 3
- 229930003451 Vitamin B1 Natural products 0.000 claims description 3
- 229930003779 Vitamin B12 Natural products 0.000 claims description 3
- 229930003471 Vitamin B2 Natural products 0.000 claims description 3
- 229930003537 Vitamin B3 Natural products 0.000 claims description 3
- 229930003776 Vitamin B4 Natural products 0.000 claims description 3
- 229930003571 Vitamin B5 Natural products 0.000 claims description 3
- 229930003756 Vitamin B7 Natural products 0.000 claims description 3
- 229930003761 Vitamin B9 Natural products 0.000 claims description 3
- 229930003427 Vitamin E Natural products 0.000 claims description 3
- 229930003448 Vitamin K Natural products 0.000 claims description 3
- 229960000643 adenine Drugs 0.000 claims description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 3
- 239000003242 anti bacterial agent Substances 0.000 claims description 3
- 229940088710 antibiotic agent Drugs 0.000 claims description 3
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 235000010216 calcium carbonate Nutrition 0.000 claims description 3
- XAAHAAMILDNBPS-UHFFFAOYSA-L calcium hydrogenphosphate dihydrate Chemical compound O.O.[Ca+2].OP([O-])([O-])=O XAAHAAMILDNBPS-UHFFFAOYSA-L 0.000 claims description 3
- UHWJJLGTKIWIJO-UHFFFAOYSA-L calcium iodate Chemical compound [Ca+2].[O-]I(=O)=O.[O-]I(=O)=O UHWJJLGTKIWIJO-UHFFFAOYSA-L 0.000 claims description 3
- 235000019390 calcium iodate Nutrition 0.000 claims description 3
- 229960002079 calcium pantothenate Drugs 0.000 claims description 3
- GFHNAMRJFCEERV-UHFFFAOYSA-L cobalt chloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].[Cl-].[Co+2] GFHNAMRJFCEERV-UHFFFAOYSA-L 0.000 claims description 3
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 claims description 3
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 3
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 3
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- XBDUTCVQJHJTQZ-UHFFFAOYSA-L iron(2+) sulfate monohydrate Chemical compound O.[Fe+2].[O-]S([O-])(=O)=O XBDUTCVQJHJTQZ-UHFFFAOYSA-L 0.000 claims description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 3
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 3
- 229940049954 penicillin Drugs 0.000 claims description 3
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims description 3
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 3
- 229960002477 riboflavin Drugs 0.000 claims description 3
- 239000011781 sodium selenite Substances 0.000 claims description 3
- 235000015921 sodium selenite Nutrition 0.000 claims description 3
- 229960001471 sodium selenite Drugs 0.000 claims description 3
- 229960003495 thiamine Drugs 0.000 claims description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 3
- 235000019155 vitamin A Nutrition 0.000 claims description 3
- 239000011719 vitamin A Substances 0.000 claims description 3
- 235000010374 vitamin B1 Nutrition 0.000 claims description 3
- 239000011691 vitamin B1 Substances 0.000 claims description 3
- 235000019163 vitamin B12 Nutrition 0.000 claims description 3
- 239000011715 vitamin B12 Substances 0.000 claims description 3
- 235000019164 vitamin B2 Nutrition 0.000 claims description 3
- 239000011716 vitamin B2 Substances 0.000 claims description 3
- 235000019160 vitamin B3 Nutrition 0.000 claims description 3
- 239000011708 vitamin B3 Substances 0.000 claims description 3
- 235000008979 vitamin B4 Nutrition 0.000 claims description 3
- 239000011579 vitamin B4 Substances 0.000 claims description 3
- 239000011675 vitamin B5 Substances 0.000 claims description 3
- 235000009492 vitamin B5 Nutrition 0.000 claims description 3
- 235000019158 vitamin B6 Nutrition 0.000 claims description 3
- 239000011726 vitamin B6 Substances 0.000 claims description 3
- 235000011912 vitamin B7 Nutrition 0.000 claims description 3
- 239000011735 vitamin B7 Substances 0.000 claims description 3
- 235000019159 vitamin B9 Nutrition 0.000 claims description 3
- 239000011727 vitamin B9 Substances 0.000 claims description 3
- 235000019165 vitamin E Nutrition 0.000 claims description 3
- 239000011709 vitamin E Substances 0.000 claims description 3
- 229940046009 vitamin E Drugs 0.000 claims description 3
- 235000019168 vitamin K Nutrition 0.000 claims description 3
- 239000011712 vitamin K Substances 0.000 claims description 3
- 150000003721 vitamin K derivatives Chemical class 0.000 claims description 3
- 229940045997 vitamin a Drugs 0.000 claims description 3
- 229940011671 vitamin b6 Drugs 0.000 claims description 3
- 229940046010 vitamin k Drugs 0.000 claims description 3
- 229940118149 zinc sulfate monohydrate Drugs 0.000 claims description 3
- RNZCSKGULNFAMC-UHFFFAOYSA-L zinc;hydrogen sulfate;hydroxide Chemical compound O.[Zn+2].[O-]S([O-])(=O)=O RNZCSKGULNFAMC-UHFFFAOYSA-L 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 230000002550 fecal effect Effects 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 235000015277 pork Nutrition 0.000 abstract description 12
- 230000000968 intestinal effect Effects 0.000 abstract description 9
- 239000000796 flavoring agent Substances 0.000 abstract description 8
- 235000019634 flavors Nutrition 0.000 abstract description 8
- 210000003205 muscle Anatomy 0.000 abstract description 8
- 239000002131 composite material Substances 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 235000019640 taste Nutrition 0.000 abstract description 4
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 10
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- 229930182817 methionine Natural products 0.000 description 8
- 239000000654 additive Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 6
- 229940088594 vitamin Drugs 0.000 description 6
- 239000011782 vitamin Substances 0.000 description 6
- 239000004471 Glycine Substances 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000000529 probiotic effect Effects 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 235000019728 animal nutrition Nutrition 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000020477 pH reduction Effects 0.000 description 3
- 238000003307 slaughter Methods 0.000 description 3
- AUHDWARTFSKSAC-HEIFUQTGSA-N (2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-2-(6-oxo-1H-purin-9-yl)oxolane-2-carboxylic acid Chemical compound [C@]1([C@H](O)[C@H](O)[C@@H](CO)O1)(N1C=NC=2C(O)=NC=NC12)C(=O)O AUHDWARTFSKSAC-HEIFUQTGSA-N 0.000 description 2
- VMDDSJXYVPDVLK-UHFFFAOYSA-N 2,3,4-trimethyl-1h-indole Chemical compound C1=CC(C)=C2C(C)=C(C)NC2=C1 VMDDSJXYVPDVLK-UHFFFAOYSA-N 0.000 description 2
- 241000606125 Bacteroides Species 0.000 description 2
- GRSZFWQUAKGDAV-UHFFFAOYSA-N Inosinic acid Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-UHFFFAOYSA-N 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229940028843 inosinic acid Drugs 0.000 description 2
- 235000013902 inosinic acid Nutrition 0.000 description 2
- 239000004245 inosinic acid Substances 0.000 description 2
- 230000007413 intestinal health Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
- 241001061264 Astragalus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241001112696 Clostridia Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241001475106 Fonticella Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010019799 Hepatitis viral Diseases 0.000 description 1
- 244000246386 Mentha pulegium Species 0.000 description 1
- 235000016257 Mentha pulegium Nutrition 0.000 description 1
- 235000004357 Mentha x piperita Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000425347 Phyla <beetle> Species 0.000 description 1
- 241000605861 Prevotella Species 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 235000019742 Vitamins premix Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229960001138 acetylsalicylic acid Drugs 0.000 description 1
- 239000002535 acidifier Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
- 229940107666 astragalus root Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004531 blood pressure lowering effect Effects 0.000 description 1
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
- 229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000001050 hortel pimenta Nutrition 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 208000023399 hyperprolinemia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 206010028417 myasthenia gravis Diseases 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 201000008752 progressive muscular atrophy Diseases 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000002320 spinal muscular atrophy Diseases 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000004233 talus Anatomy 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 201000001862 viral hepatitis Diseases 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 229940046001 vitamin b complex Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Birds (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a pig feed additive, a pig ration and application thereof, and relates to the technical field of pig feeds. The pig feed additive comprises a fermentation broth freezing drying agent and fibers, wherein the mass ratio of the fermentation broth freezing drying agent to the fibers is 1: 150-250; the effective component of the fermentation liquor freezing desiccant is endogenous compound probiotics of pigs, and the daily ration of the pigs comprises the pig feed additive, pig premix and staple food. The composite probiotics and the fibers are combined into the pig feed additive, the flora is an endogenous source, the adaptability to the intestinal environment of pigs is good, the stable and lasting colonization of the probiotics is fully ensured, and the fibers are added, so that the feed cost is reduced, and the required nutrient substances are provided for the growth and the propagation of the composite probiotics. The daily ration for pigs added with the pig feed additive can improve the muscle taste of pigs and improve the pork flavor.
Description
Technical Field
The invention relates to the technical field of pig feed, and particularly relates to a pig feed additive, a pig ration and application thereof.
Background
As main consumer goods and necessary goods of people, pork products have increasingly improved requirements on the flavor and mouthfeel of pork along with economic development.
At present, the pig breeding mode is mainly totally-enclosed large-scale breeding, and the pig daily ration is the concentrated feed processed by taking corn bean pulp as the main material, so that the pig daily ration is lack of crude fibers, the microbial diversity of pig intestinal flora is reduced, probiotics are reduced, the health condition of pigs is influenced, the content of trimethyl indole in pork is high, the population feeling is uncomfortable, and the pork quality is reduced.
In order to increase the diversity of pig intestinal microorganisms, the crude fiber can increase the abundance of pig intestinal microorganisms, but the crude fiber of the existing feed is difficult to digest, so that the live pig grows slowly and cannot meet the market demand. The addition of probiotics and suitable crude fiber feed to the daily ration of pigs can promote absorption and improve the health condition of pigs.
Chinese patent application 201910582856.7 discloses a biological compound additive feed and its application, wherein the feed comprises corn 305.35kg, soybean meal 100kg, bran 80kg, premix 10kg, peppermint, astragalus root and licorice crude extract mixture 1kg, vitamin B complex 3 kg, astragalus polysaccharide 100g, bacillus 0-300g, and organic trace elements 0-250 g. Organic trace elements are used for replacing part of inorganic trace elements, Chinese herbal medicine additives are used for improving the immunity of animal organisms, and probiotics for promoting digestion are added. The feed additive reduces the proportion of half of premix in daily ration, but does not obviously affect the apparent digestibility of 18 amino acids in the pig feed, maintains various growth performances of the fattening pigs, maintains or improves the nutrient digestibility of the feed, achieves the aim of reducing the use of antibiotics, and increases the biological safety performance of live pig products.
Chinese patent application 201811540049.0 discloses a probiotic feed additive for piglets and application and a preparation method thereof, wherein the probiotic feed additive is prepared from the following raw materials in parts by weight: 10-15 parts of probiotic powder, 20-30 parts of polysaccharide raw material, 15-20 parts of amino acid, 100 parts of soybean, 150 parts of potato, 80-100 parts of lignocellulose, 15-20 parts of a phagostimulant composition, 5-8 parts of an enzyme preparation and 5-8 parts of an acidifier. The pig feed additive provided by the invention has strong palatability, can invigorate the stomach, promote digestion, reduce the feed conversion ratio by 9.5%, obviously reduce the diarrhea rate by 95.66%, is rich in a large amount of probiotics, can help to degrade proteins and amino acids in piglets, reduce the content of ammonia gas and hydrogen sulfide gas in piglet feces, and eliminate unpleasant gas in pigsty.
The two patent applications all use commercial probiotic microbial ecological agents which are basically single bacterial strains and have no variety specificity, intestinal flora is a complex microecological system, and the intestinal flora of the same species of different varieties has larger difference, so that the application effect of the existing commercial microbial preparations has certain limitation.
In view of the above, the invention aims at the breed characteristics of pigs and combines animal nutrition to solve the problem that commercial microbial preparations lack pertinence in application.
Disclosure of Invention
The invention provides a pig feed additive, a pig ration and application thereof, aiming at the variety characteristics of pigs, combining animal nutrition, combining composite probiotics and fibers to form the pig feed additive, optimizing the proportion, developing a feeding test, and determining the optimal adding proportion of the pig feed additive in the pig feed according to the intestinal health condition and the growth performance of the pigs. Because the flora is an endogenous source, the feed additive has good adaptability to the intestinal environment of pigs, fully ensures the stable and lasting colonization of probiotics, improves the intestinal health, and simultaneously adds fiber, thereby not only reducing the feed cost, but also providing required nutrient substances for the growth and the propagation of the composite probiotics. The daily ration for pigs added with the pig feed additive can greatly improve the muscle taste of pigs and improve the pork flavor.
The technical scheme of the invention is as follows:
the pig feed additive comprises a fermentation liquor freezing drying agent and fiber, wherein the mass ratio of the fermentation liquor freezing drying agent to the fiber is 1:150-250, and preferably 1: 200.
Wherein,
the effective component of the fermentation liquor freezing desiccant is endogenous compound probiotics of pigs.
The fiber includes corn husk fiber and grass fiber.
The pig feed additive is prepared according to the following steps:
(1) selecting a donor pig;
(2) in vitro fermentation: collecting the excrement of the pig selected in the step (1), dividing the excrement into two parts, and storing one part at low temperature for high-throughput sequencing; inoculating the other part with a continuous fermentation model to obtain a stable fermentation liquid;
(3) freeze-drying the stable fermentation liquor obtained in the step (2) to obtain a fermentation liquor freeze-drying agent;
(4) and (4) mixing the fermentation liquor freezing drying agent obtained in the step (3) with fibers to obtain the pig feed additive.
Wherein,
in the step (1), the selection of the donor pig refers to that the donor pig can be obtained by culturing the fecal leachate in a culture medium added with penicillin antibiotics, and no common pathogenic bacteria colony appears in a culture dish. The penicillin antibiotic is preferably ampicillin.
In the step (2), the feces are fresh feces.
In the step (2), the low-temperature preservation temperature is-70 to-100 ℃, and preferably-80 ℃.
In the step (2), one copy of the low-temperature preservation high-throughput sequencing is used as a control for the flora structure of in vitro fermentation liquor.
In the step (2), the continuous fermentation model inoculation method comprises the following steps: single phase in vitro continuous fermentation mode.
In the step (2), the fermentation time is determined by observing the relative abundance distribution of the structures of the pig manure and the flora in the fermentation broth, and it can be seen that, at the phylum level, the donor pig cecal content and the dominant phylum in the fermentation broth are respectively Firmicutes, bacteroides and Proteobacteria, the proportion of the 3 dominant phyla is more than 80%, and the microorganisms can greatly change at the phylum level through in vitro fermentation (for example, the relative abundance of the genera such as Prevotella and bacteroides decreases, the relative abundance of the genera such as Fonticella and clostridia _1_ uncultured increases), and the change of the microorganisms in the fermentation broth tends to be stable after the fermentation is performed for a certain time, which proves that the fermentation system enters a stable operation stage, preferably 6-10 days, and further preferably 7 days.
In the step (3), the temperature of freeze drying is-20 to-40 ℃, the time is 420 and 540min, and the vacuum degree is 10 to 40 Pa; preferably, the temperature is-38 ℃, the time is 480min, and the vacuum degree is 30 Pa.
In addition, the invention also provides a daily ration for pigs, which comprises the pig feed additive, a pig premix and a staple food.
Wherein,
according to the mass percentage, the feed comprises 2 percent of pig feed additive, 4 percent of pig premix and 94 percent of staple food.
The pig premix comprises mineral trace elements and multiple vitamins, wherein the weight ratio of the mineral trace elements to the multiple vitamins is 1:1, preferably, the mineral trace elements comprise copper sulfate pentahydrate, magnesium sulfate heptahydrate, zinc sulfate monohydrate, ferrous sulfate monohydrate, manganese sulfate monohydrate, sodium selenite, cobalt chloride hexahydrate, calcium iodate, calcium carbonate, calcium hydrogen phosphate dihydrate and salt; the multivitamins include vitamin A, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B3, vitamin B4, vitamin B5, vitamin B6, vitamin B11, vitamin B12 and vitamin H.
The staple food comprises 62 parts of corn, 16 parts of soybean meal and 16 parts of bran.
The preparation method of the daily ration for the pigs comprises the following steps: the pig feed additive is prepared by processing mineral trace elements and multiple vitamins into mineral premix and multi-vitamin premix respectively, processing the mineral premix, the multi-vitamin premix, salt, calcium bicarbonate, calcium hydrogen phosphate and the like into pig premix, and processing the pig premix, the pig feed additive and staple food into pig ration.
Finally, the invention also provides application of the pig feed additive or the pig ration in improving the longissimus dorsi collagen and the umami amino acid of the pigs.
Compared with the prior art, the invention has the following beneficial characteristics:
(1) aiming at the variety characteristics of pigs, the feed additive combines animal nutrition, and combines composite probiotics and fibers into the pig feed additive, the flora is an endogenous source, the adaptability to the intestinal environment of the pigs is good, and the stable and lasting colonization of the probiotics is fully ensured;
(2) the combination of the composite probiotics and the fiber not only reduces the feed cost, but also provides required nutrient substances for the growth and the propagation of the composite probiotics.
(3) The pig ration fed with the pig feed additive can greatly improve the muscle taste of pigs and improve the pork flavor.
(4) The pig feed additive and the pig ration are particularly suitable for pigs with higher fiber nutrition requirements, and the longest muscle collagen, total delicious amino acid, methionine of the delicious amino acid and glycine of the delicious amino acid of the pigs fed by the pig feed additive are greatly improved, and the 3-methylindole is obviously reduced.
Detailed Description
The present invention will be further explained with reference to specific embodiments in order to make the technical means, the original characteristics, the achieved objects and the effects of the present invention easy to understand, but the following embodiments are only preferred embodiments of the present invention, and not all embodiments are possible. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention.
In the following examples, unless otherwise specified, all the procedures and equipment used were conventional procedures and equipment used was conventional equipment.
Example 1 and comparative examples 1 to 3
The mineral trace elements are prepared from copper sulfate pentahydrate, magnesium sulfate heptahydrate, zinc sulfate monohydrate, ferrous sulfate monohydrate, manganese sulfate monohydrate, sodium selenite, cobalt chloride hexahydrate, calcium iodate, calcium carbonate, calcium hydrogen phosphate dihydrate and salt according to a mineral premix processing technology and a pig mineral trace element requirement proportion in the pig nutritional requirement (NRC2012) for reference;
the multivitamins are prepared from vitamin A, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B3, vitamin B4, vitamin B5, vitamin B6, vitamin B11, vitamin B12 and vitamin H according to a vitamin premix processing technology and a proportion which is 1.2 times of the vitamin requirement of pig nutritional requirement (NRC 2012).
The fermentation liquor freezing desiccant is prepared according to the following method:
(1) selecting a donor: 5 healthy pigs with the optimal growth performance are selected from 80 pig groups in a pig farm of Xinkangnmu limited company in Jinhua city, excrement leachate of the 5 healthy pigs is respectively coated in a culture medium added with ampicillin to be cultured, and no common pathogenic bacteria colony appears in five culture dishes, which indicates that no common pathogenic bacteria of ampicillin resistant genes exist, so that the 5 healthy pigs all meet the requirements and serve as donor pigs.
(2) In vitro fermentation: collecting the excrement of the pig selected in the step (1), dividing the excrement into two parts, and storing one part at low temperature of-80 ℃ for high-throughput sequencing; the other part is inoculated by a continuous fermentation model, and when the fermentation is carried out for 7 days, the microorganisms in the fermentation liquor tend to be stable, so that the fermentation system is proved to enter a stable operation stage to obtain stable fermentation liquor;
(3) freeze-drying the stable fermentation liquor obtained in the step (2) under the conditions of-38 ℃, 480min and 30Pa of vacuum degree to obtain a fermentation liquor freeze-drying agent;
the fiber is corn bran fiber.
The formulations of the examples and comparative examples are shown in table 1.
TABLE 1 control, example and comparative pig ration formulas
The corresponding pig ration is processed according to the pig feed processing technology by referring to the formula in the table 1.
Result detection
50 pigs at the age of 90 days were selected, randomly divided into 5 groups of 10, and fed according to the control group, example 1, and comparative examples 1 to 3, respectively. The test period is 150 days, and indexes such as collagen, 3-methylindole, amino acid and the like in the longissimus muscle sample of the back of the pig are determined by slaughtering.
To study the effect of example 1 (enriched with complex probiotics and fiber nutrients) on meat quality, slaughter assays were performed on 8-month-old finishing pigs and the results of the relevant pork flavor index were determined as follows. The sum of 11 umami amino acids was used as comparison.
TABLE 2 comparison of the least squares means of meat quality indexes such as collagen of longissimus dorsi
As shown in Table 2, the collagen content in the longissimus dorsi of example 1 was 21.8% higher than that in the other groups (p <0.05) such as the control group. The trimethylindole in example 1 is reduced by 14.72 percent (p is less than 0.05) compared with the control group, the reduction of the comparative group is not significant and is sequentially 7.37 percent, 7.39 percent and 7.41 percent, which shows that the addition of the additive in example 1 in the daily ration of the pigs improves the muscle taste of the pigs, and the comparative group cannot realize the effect.
The comparison difference between two groups of the total sum of inosinic acid and umami amino acid is not obvious (p is greater than 0.05), but the total sum of inosinic acid and umami amino acid has an ascending trend, and the comparison examples show different degree of decline, which shows that the addition of the additive in the example 1 in daily ration can improve the pork flavor.
TABLE 3 least squares mean comparison of pH at each point determined 45-60 minutes after longissimus dorsi slaughter
As can be seen from table 3, the mean three-point pH values of example 1 showed no significant difference (p >0.05) compared to the control group, indicating that the addition of the enriched probiotic and fiber combination to the ration had no effect on the mean three-point pH values.
Compared with a control group, the shoulder pH value example 1 is reduced by 3.35% to reach 0.0581 obvious level, other comparative examples are not reduced significantly, the recommended part pH value example 1 is improved by 3.21% compared with the control group, the difference reaches 0.0678 obvious level, the comparative example is not improved significantly, the acidification degrees of the longisimus dorsi muscle of the front and rear points of the example 1 are different, after the additive of the example 1 is added into the pig ration, the shoulder acidification degree is accelerated, the recommended part acidification degree is improved, and the phenomenon is further discussed.
Table 4-1. the latest two times the mean comparison unit for the content in umami amino acid longissimus dorsi: g/100g
Table 4-2. the latest two times the mean comparison unit of the content in the umami amino acid longissimus dorsi: g/100g
Methionine has the functions of protecting cardiac muscle and resisting depression, and methionine can be converted into taurine with obvious blood pressure lowering effect. Meanwhile, methionine has good functions of protecting liver and detoxifying, so that the methionine has good effect on treating liver diseases such as liver cirrhosis, fatty liver and various acute and chronic viral hepatitis in clinic.
When the organism has serious emergency, the glycine can be used as an exogenous supplement, can also be used as a nutritional supplement, and can also play a flavoring role. The medicine is used for treating myasthenia gravis and progressive muscular atrophy, and can reduce irritation to stomach and treat hyperprolinemia in children when being combined with aspirin.
Through determination and calculation, the total amino acid content of the sample 1 is 19.95 percent, and the total amino acid content of the control group is 19.44 percent; the cumulative total of the umami amino acids in example 1 accounts for 64.68 percent of the total amino acids, and the cumulative total of the umami amino acids in the control group accounts for 64.63 percent of the total amino acids; the sum of the umami amino acids in example 1 is 12.90%, the sum of the umami amino acids in the control group is 12.56%, and the sum of the umami amino acids in example 1 is 2.7% higher than that in the control group.
As can be seen from tables 4-1 and 4-2, methionine example 1 was 15.70% higher than the control group with a significant difference (p ═ 0.0013), and glycine example 1 was 6.16% higher than the control group with a significant difference (p ═ 0.0287). The remaining amino acids example 1 were not significantly different compared to the control group (p > 0.05).
The additive in the example 1 is added into the pig ration, so that the delicious amino acids methionine and glycine in the pork are obviously increased, and the pork flavor is improved.
The data show that the pig meat quality can be obviously improved and the pork flavor is improved by adding the intestinal probiotics combined fiber nutrient into the pig daily ration.
In conclusion, the pig ration of example 1 improves the longest muscle collagen of the back of the pig by 21.8%, reduces the 3-methylindole by 14.7%, improves the total delicious amino acid by 2.7%, improves the methionine of the delicious amino acid by 15.7%, and improves the glycine of the delicious amino acid by 6.16%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The pig feed additive is characterized by comprising a fermentation liquid freezing drying agent and fiber, wherein the mass ratio of the fermentation liquid freezing drying agent to the fiber is 1: 150-250; the effective component of the fermentation liquor freezing drying agent is endogenous compound probiotics of pigs.
2. The pig feed additive according to claim 1, prepared by the steps of:
(1) selecting a donor pig;
(2) in vitro fermentation: collecting the excrement of the pig selected in the step (1), dividing the excrement into two parts, and storing one part at low temperature for high-throughput sequencing; inoculating the other part with a continuous fermentation model to obtain a stable fermentation liquid;
(3) freeze-drying the stable fermentation liquor obtained in the step (2) to obtain a fermentation liquor freeze-drying agent;
(4) and (4) mixing the fermentation liquor freezing drying agent obtained in the step (3) with fibers to obtain the pig feed additive.
3. The pig feed additive according to claim 2, wherein in the step (1), the selected donor pig is a donor pig which can be treated with pathogenic bacteria by culturing the fecal leachate in a culture medium supplemented with penicillin antibiotics.
4. The pig feed additive according to claim 2, wherein in the step (2), the fermentation time is 6-10 days.
5. The pig feed additive according to claim 2, wherein in the step (2), the continuous fermentation model is a single-phase in vitro continuous fermentation model.
6. The pig feed additive according to claim 2, wherein in the step (3), the temperature of the freeze drying is-20 to-40 ℃, the time is 420 to 540min, and the vacuum degree is 10 to 40 Pa.
7. A pig ration comprising the pig feed additive of any one of claims 1 to 6, a pig premix and a ration.
8. The pig ration according to claim 7, comprising 2% by mass of the pig feed additive, 4% by mass of the pig premix and 94% by mass of the staple food.
9. The pig ration according to claim 7, wherein the pig premix comprises mineral trace elements and multivitamins, the mineral trace elements comprising copper sulfate pentahydrate, magnesium sulfate heptahydrate, zinc sulfate monohydrate, ferrous sulfate monohydrate, manganese sulfate monohydrate, sodium selenite, cobalt chloride hexahydrate, calcium iodate, calcium carbonate, calcium hydrogen phosphate dihydrate, and salt; the multivitamin premix comprises vitamin A, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B3, vitamin B4, vitamin B5, vitamin B6, vitamin B11, vitamin B12 and vitamin H.
10. Use of a pig feed additive according to any one of claims 1 to 6 or a pig ration according to any one of claims 7 to 9 for increasing the longissimus dorsi collagen and umami amino acids in pigs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010866042.9A CN113397053B (en) | 2020-08-25 | 2020-08-25 | Pig feed additive, pig ration and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010866042.9A CN113397053B (en) | 2020-08-25 | 2020-08-25 | Pig feed additive, pig ration and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113397053A true CN113397053A (en) | 2021-09-17 |
| CN113397053B CN113397053B (en) | 2023-03-28 |
Family
ID=77677457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010866042.9A Active CN113397053B (en) | 2020-08-25 | 2020-08-25 | Pig feed additive, pig ration and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113397053B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138392A (en) * | 2007-07-30 | 2008-03-12 | 湖南农业大学 | Micro-zoology preparations for feeding |
| CN105002155A (en) * | 2015-05-26 | 2015-10-28 | 北京好实沃生物技术有限公司 | Probiotics preparation for pigs and preparation method thereof |
-
2020
- 2020-08-25 CN CN202010866042.9A patent/CN113397053B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138392A (en) * | 2007-07-30 | 2008-03-12 | 湖南农业大学 | Micro-zoology preparations for feeding |
| CN105002155A (en) * | 2015-05-26 | 2015-10-28 | 北京好实沃生物技术有限公司 | Probiotics preparation for pigs and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 项云、肖英平等: ""金华猪肠道生态菌群结构功能研究及资源化无抗安全生物饲料开发"", 《科技成果》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113397053B (en) | 2023-03-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103798553B (en) | Environment-friendly pig feed additive and preparation method thereof | |
| CN105483040A (en) | Compound biological strain for feed and preparation method of fermented feed | |
| CN104012758B (en) | biological fermentation feed and preparation method thereof | |
| CN1813562A (en) | Compound microbial fermented fodder nutrient additive and its preparing method | |
| CN106562038A (en) | Pork quality improving functional feed | |
| CN102972638A (en) | Animal feed additive and preparation method thereof | |
| CN109198214A (en) | A kind of fermented feed, preparation method and its mixed feed | |
| CN103005159A (en) | Preparation method of ginkgo leaf biological feed additive | |
| CN101756069A (en) | High-efficiency and environment-friendly feed additive for pigs and preparation method thereof | |
| KR20210043877A (en) | Method for preparing fermented total mixed ration using microbial strain complex | |
| CN102919583A (en) | Antimicrobial-free feed for young rex rabbits | |
| CN112189770A (en) | Antibiotic-free grass carp feed containing fermented mulberry leaves and preparation method thereof | |
| CN110178974B (en) | Feed additive, feed and preparation method thereof | |
| KR100541379B1 (en) | Animal feed additives and method of producing the same | |
| CN102907585A (en) | Probiotics and vitamin premix and mixture for middle pig | |
| CN102342371B (en) | Process for preparing compound feed additive from bacillus subtilis natto and bacillus licheniformis | |
| CN107373037B (en) | A kind of biological feedstuff and complete diet pellet for the black pig in the Sichuan-Tibet 30kg or more | |
| CN110150488A (en) | A kind of pig feed additive and its preparation method and application | |
| CN109984260A (en) | A kind of inactivated lactobacillus microecological agent of Rich in Trace Element and preparation method thereof | |
| CN105707439A (en) | Preparation method of animal health-care nutrient solution rich in probiotics | |
| CN117603870A (en) | Lactobacillus acidophilus for regulating and controlling animal fat metabolism and application thereof | |
| CN105231047A (en) | Low-emission environmentally-friendly feed as well as preparation method and application thereof | |
| US3243299A (en) | Monogastric feed concentrate containing rumen microorganisms and lactic ferment and process of preparation | |
| CN113397053B (en) | Pig feed additive, pig ration and application thereof | |
| CN107041456B (en) | Fermented bean dreg feed additive and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |