CN113395481A - Microscope imaging system with brightness correlation and control method thereof - Google Patents
Microscope imaging system with brightness correlation and control method thereof Download PDFInfo
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/70—Circuitry for compensating brightness variation in the scene
- H04N23/74—Circuitry for compensating brightness variation in the scene by influencing the scene brightness using illuminating means
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/80—Camera processing pipelines; Components thereof
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- H04N5/00—Details of television systems
- H04N5/222—Studio circuitry; Studio devices; Studio equipment
- H04N5/262—Studio circuits, e.g. for mixing, switching-over, change of character of image, other special effects ; Cameras specially adapted for the electronic generation of special effects
- H04N5/2624—Studio circuits, e.g. for mixing, switching-over, change of character of image, other special effects ; Cameras specially adapted for the electronic generation of special effects for obtaining an image which is composed of whole input images, e.g. splitscreen
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Abstract
The invention discloses a microscope imaging system with brightness correlation. The object carrying platform is used for placing a target object to be observed; the illumination light source is used for providing illumination brightness for the target object; the user input interface is used for providing a brightness range and a brightness adjusting value of the illumination brightness input by a user; the control unit is used for adjusting the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value input by the user; and the imaging unit is used for shooting the target object; when the control module adjusts the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value, the imaging unit can capture a plurality of images of the illumination brightness under different brightness values. The invention has the advantages that: the most clear images with different illumination brightness shot in the microscope visual field are displayed on a screen after being spliced, so that interested areas can be conveniently researched.
Description
Technical Field
The invention relates to the technical field of picture imaging, in particular to an imaging system based on a microscope shooting picture and a control method thereof.
Background
The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.
The microscope is used for researching images of various organisms or cells in a micro state, has wide application range, and is the most important instrument in the fields of biology and medicine. The microscope is a tool for observing microscopic objects, the field of view of the microscope is very limited, only a small area can be observed at the same time, and for a relatively large object or a slightly large area, the whole appearance of the object cannot be directly obtained from the microscope, so that the microscope needs to be continuously adjusted to obtain images of different areas, but the panoramic observation in one field of view cannot be usually realized.
In addition, since the microscope is a technique that requires the inside of an object to be viewed with light and the illumination brightness suitable for each object is different in practice, it is difficult to directly obtain a clear photograph due to the structure of the conventional microscope itself. Without a clear figure, all subsequent studies became meaningless.
It should be noted that the above background description is only for the sake of clarity and complete description of the technical solutions of the present invention and for the understanding of those skilled in the art. Such solutions are not considered to be known to the person skilled in the art merely because they have been set forth in the background section of the invention.
Disclosure of Invention
In order to overcome the defects in the prior art, embodiments of the present invention provide a brightness-dependent microscope imaging system and a control method thereof, where the brightness-dependent microscope imaging system can panorama the texture and the overall view of different areas of a studied object under different illumination brightness.
The embodiment of the application discloses: a microscope imaging system with brightness correlation comprises a carrying platform, an illumination light source, a user input interface, a control unit and an imaging unit. The object carrying platform is used for placing a target object to be observed; the illumination light source is positioned above the object carrying platform and used for providing illumination brightness for the object; the user input interface is used for providing a brightness range and a brightness adjusting value of the illumination brightness input by a user, wherein the brightness range comprises a maximum brightness value and a minimum brightness value; the control unit is coupled to the illumination light source and the user input interface, and is configured to adjust the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value input by the user; the imaging unit is used for shooting the target object so as to generate a plurality of images in each second; when the control module adjusts the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value, the imaging unit can capture a plurality of images of the illumination brightness under different brightness values.
Further, the control unit may first adjust the illumination brightness of the illumination source to the maximum brightness value, and may decrease the illumination brightness of the illumination source according to the brightness adjustment value after every predetermined time interval until the illumination brightness of the illumination source is adjusted to the minimum brightness value.
Further, the brightness-dependent microscope imaging system further comprises: a calculating unit, coupled to the imaging unit, for calculating the sharpness of the plurality of images; and a determining unit, coupled to the calculating unit, for determining a clearest image from the plurality of images.
Further, the imaging unit may divide the target object into mxn regions, and a photograph may be taken for each regionpA sheet of block image, wherein said each regionpThe tile images are captured at the same brightness value of the illumination brightness.
Further, the determining unit defaults a first image as a main image to be finally output, and then calculates for each regionpThe definition of a specific block image is greater than the highest definition of the region on the main imageThe highest sharpness value is updated while the default block image of the region on the main picture image is replaced with the specific block image.
Further, the calculating unit calculates the sharpness of the plurality of images using a first function, which is expressed by:
D(f)＝∑y∑x|f(x+2，y)-f(x，y)|2 (1)：
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
Further, the calculating unit calculates the sharpness of the plurality of images using a second function, which is expressed by:
D(f)＝∑y∑x(|f(x+1，y)-f(x，y)|2+|f(x，y+1)-f(x，y)|2) (2)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
Further, the calculating unit calculates the sharpness of the plurality of images using a third function, which is expressed by:
D(f)＝∑y∑x(|f(x，y)-f(x，y-1)|+|f(x，y)-f(x+1，y)|) (3)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
Further, the calculation unit calculates the sharpness of the block image corresponding to a first group of regions using the first function, calculates the sharpness of the block image corresponding to a second group of regions using the second function, and calculates the sharpness of the block image corresponding to a third group of regions using the third function.
The embodiment of the application discloses: a method of controlling a brightness-dependent microscopy imaging system including an illumination source for providing an illumination brightness to a subject and an imaging unit for capturing images of the subject to produce a plurality of images per second. The method comprises the following steps: providing a user input interface for a user to input a brightness range and a brightness adjustment value of the illumination brightness, wherein the brightness range comprises a maximum brightness value and a minimum brightness value; adjusting the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value input by the user; and controlling the imaging unit to capture a plurality of images of the illumination brightness under different brightness values when the illumination brightness of the illumination light source is adjusted according to the brightness range and the brightness adjustment value.
By means of the technical scheme, the invention has the following beneficial effects: the clearest images with different illumination brightness shot in the microscope visual field are displayed on a screen after being spliced, so that the overall view of the object to be researched in the microscopic visual field can be conveniently checked, and the interested area can be conveniently researched.
In order to make the aforementioned and other objects, features and advantages of the invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic diagram of a brightness dependent microscopy imaging system in a first embodiment of the invention.
FIG. 2 is a block diagram of a brightness dependent microscopy imaging system in a first embodiment of the invention.
FIG. 3 is a block diagram of a brightness dependent microscopy imaging system according to a second embodiment of the present invention.
Fig. 4 is a schematic view of an image taken by the imaging unit.
FIG. 5 is a flow chart of a method of controlling a brightness dependent microscopy imaging system in an embodiment of the invention.
Reference numerals of the above figures: 10. 30, a brightness-dependent microscope imaging system; 110. a carrier platform; 120. an illumination light source; 130. a user input interface; 140. a control unit; 150. an imaging unit; 360. a calculation unit; 370. a determination unit; OB1, target; r, brightness range; Δ X, brightness adjustment value; MAX, maximum brightness value; MIN, minimum brightness value; AREA 11-AREA 45, AREA; s510, S520, S530 and step.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, in the description of the present invention, the terms "first", "second", and the like are used for descriptive purposes only and for distinguishing similar objects, and no precedence between the two is considered as indicating or implying relative importance. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Please refer to fig. 1 and fig. 2. Fig. 1 is a schematic diagram of a brightness-dependent microscopy imaging system 10 according to a first embodiment of the present invention, and fig. 2 is a block diagram of a brightness-dependent microscopy imaging system 10 according to a first embodiment of the present invention. As shown in fig. 1 and 2, a microscope imaging system 10 with brightness correlation includes a stage 110, an illumination source 120, a user input interface 130, a control unit 140, and an imaging unit 150. The object stage 110 is used for placing an object OB1 to be observed; the illumination source 120 is located above the stage 110 and is used for providing an illumination intensity to the object OB 1. The user input interface 130 is used for providing a user with an input of a brightness range R and a brightness adjustment value Δ X of the illumination brightness, wherein the brightness range R includes a maximum brightness value MAX and a minimum brightness value MIN. The control unit 140 is coupled to the illumination source 120 and the user input interface 130, and configured to adjust the illumination brightness of the illumination source 120 according to the brightness range R and the brightness adjustment value Δ X input by the user. The imaging unit 150 is used to capture the object OB1 to generate a plurality of images per second. When the control unit 140 adjusts the illumination brightness of the illumination source 120 according to the brightness range R and the brightness adjustment value Δ X, the imaging unit 150 can capture a plurality of images of the illumination brightness at different brightness values, which is only an example and is not a limitation of the present invention.
In one possible embodiment, the illumination source 120 may comprise R, G, B LED light source, or white LED light source LED, and the illumination source 120 modulates the frequency of 40 kHz.
In one possible embodiment, the control unit 140 first adjusts the illumination intensity of the illumination source 120 to the maximum intensity value MAX, and then gradually decreases the illumination intensity of the illumination source 120 according to the intensity adjustment value after every predetermined time interval until the illumination intensity of the illumination source 120 is adjusted to the minimum intensity value MIN. For example, the user may set the maximum brightness value of the brightness range R of the illumination brightness to 255, the minimum brightness value to 0, and the brightness adjustment value Δ X to 5, so that the control unit 140 first adjusts the illumination brightness of the illumination light source 120 to 255, and the imaging unit 150 captures a plurality of images of the object OB1 corresponding to the illumination brightness 255; then, after every predetermined time interval (for example, every 30 seconds or after the imaging unit 150 has captured all the images of the object OB 1), the control unit 140 first decrements the illumination intensity of the illumination light source 120 by 5 (the intensity adjustment value Δ X) each time, and sequentially decrements and adjusts the illumination intensity to 250, 245, 240, 235 … until the illumination intensity of the illumination light source 120 is adjusted to 0 (the minimum intensity value MIN). At this time, the imaging unit 150 also captures a plurality of images of the object OB1 corresponding to the illumination intensities 250, 245, 240, 235 …. The maximum brightness value, the minimum brightness value, the brightness adjustment value and the predetermined time interval are only exemplary and are not limitations of the present invention. The setting values can be designed into different values according to actual requirements, and the scope of the invention is also covered by the invention.
Referring to fig. 3, fig. 3 is a block diagram of a brightness-dependent microscope imaging system 30 according to a second embodiment of the present invention. The illumination-dependent microscopy imaging system 30 of fig. 3 is similar to the illumination-dependent microscopy imaging system 10 of fig. 2, except that the illumination-dependent microscopy imaging system 30 further comprises a calculating unit 360 and a determining unit 370. A calculating unit 360 is coupled to the imaging unit 150 for calculating the sharpness of the plurality of images. The determining unit 370 is coupled to the calculating unit 360 for determining a clearest image from the plurality of images.
Referring to fig. 4, fig. 4 is a schematic diagram of an image captured by the imaging unit 150. As shown in fig. 3, the imaging unit 150 divides the object OB1 into mxn regions (e.g., 3x5 regions AREA 11-AREA 35, each region size is 16 × 16), and takes p block images for each region (e.g., the first region AREA11), wherein the p block images of each region are captured under the same brightness value of the illumination brightness. In the above example, when the maximum luminance value MAX is 255, the minimum luminance value MIN is 0, and the luminance adjustment value Δ X is 5 (52 luminance values), 10400(52X200) block images of the first AREA11 are captured in a total, and the 10400 block images correspond to different luminance values (255, 250, 245, 240 … 10, 5, 0), respectively. In analogy, 10400 block images of the second AREA11 are captured for the second AREA12 until all the AREAs (including 3 × 5 AREAs AREA11 to AREA35) are completely captured with 200 block images. That is, a total of mxnxp block images are obtained at each illumination brightness value, and in the above example, a total of 5 × 3 × 200 block images are captured at each illumination brightness value.
It should be noted that m, n, and p are only exemplary and not limiting. The setting values can be designed into different values according to actual requirements, and the scope of the invention is also covered by the invention.
It should be noted that, in the above embodiment, each of the mxn regions uses the same luminance range R and the same luminance adjustment value Δ X for capturing the block image, which is only an example and not a limitation of the present invention. In other embodiments, the different regions may use different luminance ranges R (including the maximum luminance value MAX and the minimum luminance value MIN) or different luminance adjustment values Δ X for capturing the block image. In this case, different brightness ranges R (including the maximum brightness value MAX and the minimum brightness value MIN) or different brightness adjustment values Δ X are input for the respective regions. For example, the maximum brightness value MAX of the AREA11 may be 255, the minimum brightness value MIN may be 0, the brightness adjustment value Δ X may be 5, the maximum brightness value MAX of the AREA12 may be 200, the minimum brightness value MIN may be 100, the brightness adjustment value Δ X may be 5, the maximum brightness value MAX of the AREA13 may be 150, the minimum brightness value MIN may be 100, the brightness adjustment value Δ X may be 5, and so on, so that different AREAs may correspond to different brightness ranges R (different maximum brightness values MAX and minimum brightness values MIN).
In one possible embodiment, the brightness range R of the illumination brightness may include a maximum brightness value MAX and a number N. For example, the user may set the maximum brightness value of the brightness range R of the illumination brightness to 255, set the number of times N to 10, and set the brightness adjustment value Δ X to 5, so that the control unit 140 first adjusts the illumination brightness of the illumination light source 120 to 255, and the imaging unit 150 captures a plurality of images of the object OB1 corresponding to the illumination brightness 255; then, after every predetermined time interval (for example, every 30 seconds or after the imaging unit 150 has captured all the images of the object OB 1), the control unit 140 first decrements the illumination intensity of the illumination light source 120 by 5 (the intensity adjustment value Δ X) each time, and sequentially decrements and adjusts the illumination intensity to 250, 245, 240, 235 … 210 until the illumination intensity of the illumination light source 120 is adjusted to 210 (the total number of execution times is 10).
In the determination process, firstly, the determining unit 370 defaults a first image as a main image to be finally output, then calculates the sharpness of p block images for each region calculating unit 360, and if the sharpness of a specific block image is greater than the highest sharpness of the region on the main image, updates the highest sharpness value, and simultaneously replaces the default block image of the region on the main image with the specific block image. For example, in the case of a brightness value of 255, the determining unit 370 first defaults that the first block images of all the AREAs AREA 11-AREA 35 are the final output main image, and the first block images of all the AREAs correspond to the brightness value of 255. Then, for the first AREA11, the calculating unit 360 calculates the sharpness of 200 block images, and if the sharpness of a specific block image is greater than the highest sharpness of the first AREA11 on the main image, the highest sharpness value is updated, and the determining unit 370 replaces the default block image (i.e., the first block image) of the first AREA11 on the main image with the specific block image. When the sharpness of all the 200 block images of the first AREA11 is compared, the determining unit 370 determines a sharpest block image. By analogy, for all the AREAs AREA11 to AREA35, the calculating unit 360 calculates the sharpness of 200 block images, and the final determining unit 370 determines the clearest block images of each of the AREAs AREA11 to AREA35, and combines the clearest block images into the final output main image. Thus, there is one clearest main image under each brightness value, and in the above example, the invention will generate 52 clearest main images (52 brightness values). Each main image is spliced by the clearest AREAs AREA 11-AREA 35.
In one possible embodiment, the invention calculates the square of the difference between two adjacent pixels to calculate the sharpness of the image, that is, the calculating unit 360 may calculate the sharpness of the plurality of images using a first function, where the first function is:
D(f)＝∑y∑x|f(x+2，y)-f(x，y)|2 (1)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
In another possible embodiment, the invention calculates the sharpness of the image by calculating the energy gradient, that is, the calculating unit 360 may calculate the sharpness of the plurality of images by using a second function, where the second function is:
D(f)＝∑y∑x(|f(x+1，y)-f(x，y)|2+|f(x，y+1)-f(x，y)|2) (2)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
In another possible embodiment, the invention calculates the sharpness of the image by multiplying two gray differences in each pixel region and then accumulating the multiplied differences one by one, that is, the calculating unit 360 may calculate the sharpness of the plurality of images by using a third function, where the third function is:
D(f)＝∑y∑x(|f(x，y)-f(x，y-1)|+|f(x，y)-f(x+1，y)|) (3)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
It should be understood by those skilled in the art that the above-mentioned first function, second function and third function can be used individually or simultaneously, and are within the scope of the present invention. In a possible embodiment of the present invention, the calculating unit calculates the sharpness of the block image corresponding to a first group of regions using the first function, calculates the sharpness of the block image corresponding to a second group of regions using the second function, and calculates the sharpness of the block image corresponding to a third group of regions using the third function. For example, the first function may be used to calculate the plurality of block images corresponding to the AREAs AREA 11-AREA 15, the second function may be used to calculate the plurality of block images corresponding to the AREAs AREA 21-AREA 25, and the third function may be used to calculate the plurality of block images corresponding to the AREAs AREA 31-AREA 35, which are only exemplary and not limitations of the present invention.
Please refer to the following Table _1, where Table _1 represents the highest definition obtained by calculating the plurality of block images in different areas by using the first function, the second function, and the third function, respectively. As can be seen from Table _1, the AREAs AREA11 to AREA15 have the highest sharpness (880) obtained by calculating the plurality of block images by using the first function, the AREAs AREA21 to AREA25 have the highest sharpness (822) obtained by calculating the plurality of block images by using the second function, and the AREAs AREA31 to AREA35 have the highest sharpness (937) obtained by calculating the plurality of block images by using the third function.
Table_1
Referring to fig. 5, fig. 5 is a flowchart illustrating a method for controlling a brightness-dependent microscope imaging system according to an embodiment of the present invention. The microscope imaging system comprises an illumination light source for providing illumination brightness to a target object and an imaging unit for shooting the target object to generate a plurality of images in each second. The method comprises the following steps:
s510: providing a user input interface for a user to input a brightness range and a brightness adjustment value of the illumination brightness, wherein the brightness range comprises a maximum brightness value and a minimum brightness value;
s520: adjusting the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value input by the user; and
s530: when the illumination brightness of the illumination light source is adjusted according to the brightness range and the brightness adjustment value, the imaging unit is controlled to capture a plurality of images of the illumination brightness under different brightness values.
By means of the technical scheme, the invention has the following beneficial effects: the clearest images with different illumination brightness shot in the microscope visual field are displayed on a screen after being spliced, so that the overall view of the object to be researched in the microscopic visual field can be conveniently checked, and the interested area can be conveniently researched.
The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (10)
1. A brightness dependent microscopy imaging system comprising:
the object carrying platform is used for placing a target object to be observed;
the illumination light source is positioned above the object platform and used for providing illumination brightness for the object;
a user input interface for providing a brightness range and a brightness adjustment value for a user to input the illumination brightness, wherein the brightness range includes a maximum brightness value and a minimum brightness value;
a control unit, coupled to the illumination source and the user input interface, for adjusting the illumination brightness of the illumination source according to the brightness range and the brightness adjustment value input by the user; and
an imaging unit for photographing the target object to generate a plurality of images per second;
when the control unit adjusts the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value, the imaging unit can capture a plurality of images of the illumination brightness under different brightness values.
2. The system of claim 1, wherein the control unit adjusts the illumination intensity of the illumination source to the maximum intensity value and adjusts the illumination intensity of the illumination source in a decreasing manner according to the intensity adjustment value after a predetermined time interval until the illumination intensity of the illumination source is adjusted to the minimum intensity value.
3. The brightness-dependent microscopy imaging system of claim 1, further comprising:
a calculating unit, coupled to the imaging unit, for calculating the sharpness of the plurality of images; and
a determining unit, coupled to the calculating unit, for determining a clearest image from the plurality of images.
4. The brightness-dependent microscopy imaging system of claim 3, wherein the imaging unit divides the object into mxn regions, and p block images are captured for each region, wherein the p block images of each region are captured at the same brightness value of the illumination brightness.
5. The brightness-dependent microscopy imaging system of claim 4, wherein the determining unit defaults a first image as a final output main image, and then for each region, the calculating unit calculates the sharpness of p block images, and if the sharpness of a particular block image is greater than the highest sharpness of the region on the main image, the highest sharpness value is updated, and the determining unit replaces the default block image of the region on the main image with the particular block image.
6. The brightness dependent microscopy imaging system of claim 3, wherein the calculating unit calculates the sharpness of the plurality of images using a first function, the first function being formulated as:
D(f)＝∑y∑x|f(x+2，y)-f(x，y)|2 (1)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
7. The brightness dependent microscopy imaging system of claim 3, wherein the calculating unit calculates the sharpness of the plurality of images using a second function, the second function being formulated as:
D(f)＝∑y∑x(|f(x+1，y)-f(x，y)|2+|f(x，y+1)-f(x，y)|2) (2)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
8. The brightness dependent microscopy imaging system of claim 3, wherein the calculating unit calculates the sharpness of the plurality of images using a third function, the third function being formulated as:
D(f)＝∑y∑x(|f(x，y)-f(x，y-1)|+|f(x，y)-f(x+1，y)|) (3)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
9. The brightness-dependent microscopy imaging system of any of claims 6-8, wherein the calculation unit employs the first function to calculate the sharpness of block images corresponding to a first set of regions, the second function to calculate the sharpness of block images corresponding to a second set of regions, and the third function to calculate the sharpness of block images corresponding to a third set of regions.
10. A method of controlling a brightness-dependent microscopy imaging system, the brightness-dependent microscopy imaging system including an illumination source for providing an illumination brightness to a subject and an imaging unit for capturing the subject to produce a plurality of images per second, the method comprising the steps of:
providing a user input interface for a user to input a brightness range and a brightness adjustment value of the illumination brightness, wherein the brightness range comprises a maximum brightness value and a minimum brightness value;
adjusting the illumination brightness of the illumination light source according to the brightness range and the brightness adjustment value input by the user; and
when the illumination brightness of the illumination light source is adjusted according to the brightness range and the brightness adjustment value, the imaging unit is controlled to capture a plurality of images of the illumination brightness under different brightness values.
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| CN (1) | CN113395481A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115877555A (en) * | 2023-02-13 | 2023-03-31 | 南京木木西里科技有限公司 | Microscope illumination simulation system |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040101169A1 (en) * | 2002-11-20 | 2004-05-27 | Stmicroelectronics S.A. | Determination of a definition score of a digital image |
| CN101441320A (en) * | 2008-12-09 | 2009-05-27 | 东华大学 | High dynamic image acquisition device based on microscopic imaging detection and method thereof |
| CN102968792A (en) * | 2012-10-29 | 2013-03-13 | 中国科学院自动化研究所 | Method for multi-focal-plane object imaging under microscopic vision |
| JP2016003906A (en) * | 2014-06-16 | 2016-01-12 | 大日本印刷株式会社 | Device and method for measuring sharpness |
| CN106331454A (en) * | 2016-08-31 | 2017-01-11 | 北京寺库商贸有限公司 | Microscope precision picture taking system and picture taking method |
| CN109085113A (en) * | 2018-07-05 | 2018-12-25 | 深圳辉煌耀强科技有限公司 | A kind of Atomatic focusing method and device for cervical exfoliated cell detection device |
| CN110146974A (en) * | 2019-05-29 | 2019-08-20 | 电子科技大学 | A kind of intelligent biological microscope |
| CN210053476U (en) * | 2019-08-13 | 2020-02-11 | 苏州瑞霏光电科技有限公司 | Array reflection type microscopic image acquisition system |
-
2020
- 2020-03-12 CN CN202010170392.1A patent/CN113395481A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040101169A1 (en) * | 2002-11-20 | 2004-05-27 | Stmicroelectronics S.A. | Determination of a definition score of a digital image |
| CN101441320A (en) * | 2008-12-09 | 2009-05-27 | 东华大学 | High dynamic image acquisition device based on microscopic imaging detection and method thereof |
| CN102968792A (en) * | 2012-10-29 | 2013-03-13 | 中国科学院自动化研究所 | Method for multi-focal-plane object imaging under microscopic vision |
| JP2016003906A (en) * | 2014-06-16 | 2016-01-12 | 大日本印刷株式会社 | Device and method for measuring sharpness |
| CN106331454A (en) * | 2016-08-31 | 2017-01-11 | 北京寺库商贸有限公司 | Microscope precision picture taking system and picture taking method |
| CN109085113A (en) * | 2018-07-05 | 2018-12-25 | 深圳辉煌耀强科技有限公司 | A kind of Atomatic focusing method and device for cervical exfoliated cell detection device |
| CN110146974A (en) * | 2019-05-29 | 2019-08-20 | 电子科技大学 | A kind of intelligent biological microscope |
| CN210053476U (en) * | 2019-08-13 | 2020-02-11 | 苏州瑞霏光电科技有限公司 | Array reflection type microscopic image acquisition system |
Non-Patent Citations (3)
| Title |
|---|
| 徐东明等: "显微标本无损分析仪的研究", 《应用光学》 * |
| 李伊文等: "钢球表面缺陷的电脑显微观测", 《轴承》 * |
| 李雪 等: "光学显微成像系统图像清晰度评价函数的对比", 《光学仪器》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115877555A (en) * | 2023-02-13 | 2023-03-31 | 南京木木西里科技有限公司 | Microscope illumination simulation system |
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