CN113390868A - Method and system for presenting target microscopic image - Google Patents
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Abstract
It is an object of the present application to provide a method and system for presenting a microscopic image of an object; the method comprises the following steps: acquiring a plurality of target microscopic images related to a target sample, wherein each target microscopic image corresponds to an instant temperature respectively; generating a temperature sequence based on each instant temperature; and sequentially presenting the plurality of target microscopic images based on the temperature sequence. This application can improve researcher's work efficiency.
Description
Technical Field
The present application relates to the field of microscopic imaging, and more particularly, to a technique for presenting a microscopic image of a target.
Background
Microscopic Optical imaging, sometimes also referred to as "Optical Microscopy" or "Optical Microscopy," refers to a technique for obtaining magnified images of a microscopic sample based on visible Light transmitted through or reflected from the sample through one or more lenses. The obtained image can be directly observed by eyes through an ocular lens, and can also be recorded by a light-sensitive plate or a digital image detector (such as CCD, CMOS and the like); the resulting image may also be displayed and processed for analysis on a computer. In some cases, researchers may conduct experiments based on microscopic optical imaging systems, such as continuously observing the changes of a sample over time, and recording, studying.
Disclosure of Invention
It is an object of the present application to provide a method and system for presenting a microscopic image of an object.
According to one aspect of the present application, a method for presenting a microscopic image of an object is provided. Wherein, the method comprises the following steps:
acquiring a plurality of target microscopic images related to a target sample, wherein each target microscopic image corresponds to an instant temperature respectively;
generating a temperature sequence based on each instant temperature;
sequentially presenting the plurality of target microscopic images based on the temperature sequence.
Accordingly, the present application also provides a microscope system for presenting a microscopic image of an object, the system comprising:
a processor; and
a memory arranged to store computer executable instructions that, when executed, cause the processor to perform the operations of the above-described method.
Also provided is a computer-readable medium storing instructions that, when executed by a computer, cause the computer to perform the operations of the above-described method.
According to another aspect of the present application, there is also provided an apparatus for presenting a microscopic image of an object. Wherein, the device includes:
a first module for obtaining a plurality of target microscopic images about a target sample, wherein each target microscopic image corresponds to an instant temperature;
a second module for generating a temperature sequence based on each instant temperature; and
a third module for sequentially presenting the plurality of target microscopic images based on the temperature sequence.
Compared with the prior art, the method and the device have the advantages that after the plurality of target microscopic images of the target sample are obtained, the target microscopic images are presented based on the temperature sequence. Therefore, a researcher does not need to stay beside the microscopic equipment in the test process in order to research the change of the sample along with the temperature change, a large amount of time can be saved for the researcher, and the researcher is not easy to miss key time points due to forgetting to observe; in addition, based on customized observation for different temperatures (for example, repeatedly comparing images at different temperatures, or observing the sample according to the temperature variation trend different from the test process), the researcher can observe the corresponding variation trend of the sample without arranging a plurality of different tests. Furthermore, based on the present application, different researchers can reproduce the temperature-dependent change of the sample based on the acquired target microscopic image without the need to repeat the related experiments.
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Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:
FIG. 1 illustrates an implementation scenario of one embodiment of the present application;
FIG. 2 is a flow chart of a method for presenting a target microscopic image in one embodiment of the present application;
FIG. 3 is an illustration of determining relative spatial positions based on focal plane heights in an embodiment of the present application;
FIG. 4 illustrates functional modules of a system for presenting a target microscopic image in one embodiment of the present application;
FIG. 5 illustrates a functional block diagram of an exemplary system that may be used in various embodiments of the present application.
The same or similar reference numbers in the drawings identify the same or similar elements.
Detailed Description
The present application is described in further detail below with reference to the attached figures.
In a typical configuration of the present application, the terminal, the device serving the network, and the trusted party each include one or more processors (e.g., Central Processing Units (CPUs)), input/output interfaces, network interfaces, and memory.
The Memory may include forms of volatile Memory, Random Access Memory (RAM), and/or non-volatile Memory in a computer-readable medium, such as Read Only Memory (ROM) or Flash Memory. Memory is an example of a computer-readable medium.
Computer-readable media, including both non-transitory and non-transitory, removable and non-removable media, may implement information storage by any method or technology. The information may be computer readable instructions, data structures, modules of a program, or other data. Examples of computer storage media include, but are not limited to, Phase-Change Memory (PCM), Programmable Random Access Memory (PRAM), Static Random Access Memory (SRAM), Dynamic Random Access Memory (DRAM), other types of Random Access Memory (RAM), Read-Only Memory (ROM), Electrically Erasable Programmable Read-Only Memory (Electrically-Erasable Programmable Read-Only Memory (EEPROM), Flash Memory (Flash Memory) or other Memory technology, Compact Disc Read-Only Memory (CD-ROM), digital versatile Disc (DVD-ROM), or any other magnetic storage device, magnetic tape, or magnetic tape or other non-magnetic storage device, may be used to store information that may be accessed by the computing device.
The device referred to in this application includes, but is not limited to, a user device, a network device, or a device formed by integrating a user device and a network device through a network. The user equipment includes, but is not limited to, any mobile electronic product, such as a smart phone, a tablet computer, etc., capable of performing human-computer interaction with a user (e.g., human-computer interaction through a touch panel), and the mobile electronic product may employ any operating system, such as an Android operating system, an iOS operating system, etc. The network Device includes an electronic Device capable of automatically performing numerical calculation and information processing according to a preset or stored instruction, and the hardware includes, but is not limited to, a microprocessor, an Application Specific Integrated Circuit (ASIC), a Programmable Logic Device (PLD), a Field Programmable Gate Array (FPGA), a Digital Signal Processor (DSP), an embedded Device, and the like. The network device includes but is not limited to a computer, a network host, a single network server, a plurality of network server sets or a cloud of a plurality of servers; here, the Cloud is composed of a large number of computers or web servers based on Cloud Computing (Cloud Computing), which is a kind of distributed Computing, one virtual supercomputer consisting of a collection of loosely coupled computers. Including, but not limited to, the internet, a wide area Network, a metropolitan area Network, a local area Network, a VPN Network, a wireless Ad Hoc Network (Ad Hoc Network), etc. Preferably, the device may also be a program running on the user device, the network device, or a device formed by integrating the user device and the network device, the touch terminal, or the network device and the touch terminal through a network.
Of course, those skilled in the art will appreciate that the foregoing is by way of example only, and that other existing or future devices, which may be suitable for use in the present application, are also encompassed within the scope of the present application and are hereby incorporated by reference.
In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
Embodiments of the present application are implemented based on a microscope system having an optical assembly (e.g., an objective lens) for imaging a sample and a data processing assembly for receiving, processing, and transmitting data. Without limitation, fig. 1 illustrates one exemplary implementation scenario of the embodiments of the present application. The microscope system 10 comprises a microscope device 11 for imaging the sample and a computer device 12 for processing data and sending motion instructions to the microscope device 11. The microscope device 11 and the computer device 12 are connected by a communication cable for providing a communication line between the microscope device 11 and the computer device 12 so that the microscope device 11 sends a microscope image to the computer device 12, and the computer device 12 detects the state of the microscope device 11, sends an action instruction to the microscope device 11, and the like. The microscope device 11 includes a working assembly 11a (which may be an objective lens assembly or a working assembly for performing other operations according to actual needs), an object stage 11b, and the object stage 11b is used for placing or holding a sample 11 c; taking the working assembly 11a as an example of an objective lens assembly, the microscope device 11 receives the instruction from the computer device 12 to move the working assembly 11a and the stage 11b relatively, so that the working assembly 11a images different parts of the sample 11c, and sends the obtained microscopic image to the computer device 12 through the communication cable. In an exemplary embodiment, stage 11b moves in the x-y plane of FIG. 1, while working assembly 11a moves in the z-direction, and the driving force for the moving parts is provided by a stepper motor and screw mechanism.
It will be understood by those skilled in the art that the foregoing embodiments are by way of example only, and are not limiting of the present application. Other existing embodiments that enable relative movement between the sample and the working assembly, as applicable to the present application, are also within the scope of the present application and are incorporated by reference herein.
A detailed description of the present application will be given below based on the microscope system shown in fig. 1.
According to one aspect of the present application, a method for presenting a microscopic image of an object is provided. Referring to fig. 2, the method includes step S100, step S200, and step S300. In step S100, the microscope system acquires a plurality of target microscopic images of a target sample, wherein each target microscopic image corresponds to an instant temperature; in step S200, the microscope system generates a temperature sequence based on each instant temperature; in step S300, the microscope system sequentially presents the plurality of target microscopic images based on the temperature sequence.
Specifically, in step S100, the microscope system acquires a plurality of target microscopic images with respect to a target specimen. In some embodiments, the microscope system further comprises a temperature control unit (not shown); based on the temperature control unit, the microscope system collects microscopic images of a target sample as target microscopic images based on a plurality of preset target temperatures; alternatively, the target temperatures are respectively spaced by the same or different temperature differences. In some embodiments, the temperature control unit detects a current instant temperature, and sends an image acquisition instruction to the computer device when the instant temperature satisfies a temperature condition (for example, a temperature difference between the instant temperature and a previous instant temperature reaches a preset, same or different temperature difference, or the instant temperature is in a preset instant temperature list), so as to acquire a corresponding target microscopic image corresponding to the instant temperature; in other embodiments, the temperature control unit controls the instant temperature to change, and sends an image acquisition instruction to the computer device to acquire the target microscopic image when the instant temperature satisfies a temperature condition (e.g., a temperature difference between the instant temperature and a previous instant temperature reaches a preset, same or different temperature difference, or the instant temperature is in a preset instant temperature list). Wherein, the instant temperature can be, but is not limited to, a sample temperature, an ambient temperature, a slide temperature, etc.; in some embodiments the instantaneous temperature is measured contactlessly (e.g., based on a thermometer or thermocouple) or contactlessly (e.g., based on a brightness method, a radiation method, or a colorimetric method).
In step S200, the microscope system generates a temperature sequence based on each instant temperature; subsequently, in step S300, the microscope system sequentially presents the plurality of target microscopic images based on the temperature sequence, for example sequentially presents the target microscopic image corresponding to each instant temperature based on the temperature sequence (for example, a certain target microscopic image is acquired at a certain instant temperature, and the target microscopic image corresponds to the instant temperature). In some embodiments, the instant temperatures in the temperature sequence are arranged according to the change of the instant temperatures in the test process, so that the sequentially presented target microscopic images can display the dynamic process of the change of the sample in the test, so that researchers can research the test process; in other embodiments, the instant temperatures in the temperature sequence are arranged according to the arrangement instruction of a user (researcher), so that the sequentially presented targets are limited to the tendency that the images can present changes along with the temperature changes according to the requirements of the user, the convenience of the microscope system is greatly expanded, and the operation efficiency of the user is greatly improved.
Specifically, in some embodiments, in the step S200, the microscope system arranges the instant temperatures according to a preset temperature arrangement rule to generate a corresponding temperature sequence, so as to present a dynamic process of the target microscopic image varying with the temperature for research; accordingly, the order of presentation of the target microscopic images is also determined by the temperature arrangement rule. In some embodiments, the microscope system automatically generates a corresponding temperature sequence based on the temperature arrangement rule and presents the target microscope images in a corresponding order. For example, the microscope system sequentially presents the target microscopic images based on the photographing time of each target microscopic image, or sequentially presents the corresponding target microscopic images based on the instant temperatures in the ascending/descending order. In some embodiments, in step S200, the microscope system generates a corresponding temperature sequence in response to a temperature arrangement operation (for example, a dragging operation of the user in the visual interface) performed by the user on each instant temperature, so as to meet a customized presentation requirement of the user and expand convenience of the microscope system.
In some embodiments, step S300 described above includes sub-step S310 and sub-step S320 (both not shown). In sub-step S310, the microscope system generates respective image sequences for the plurality of target microscopic images based on the temperature sequence; in sub-step S320, the microscope system sequentially presents the plurality of target microscope images based on the image sequence. In some embodiments, in step S310, the microscope system generates an image sequence corresponding to the temperature sequence based on the correspondence between each instant temperature and the corresponding target microscopic image. Therefore, the microscope system firstly arranges the target microscopic images according to the change of each instant temperature along with time to obtain the change of the microscopic images along with time so as to obtain an image sequence related to time, and then presents the microscopic images in sequence based on the image sequence so as to repeatedly present the change of the target microscopic images along with time in the following process, and the corresponding images do not need to be read according to the mapping of the target microscopic images and the instant temperatures every time, so that the response speed of the system to the repeated comparison and research of a researcher is improved.
In some embodiments, in the step S320, the microscope system sequentially presents the plurality of target microscopic images based on a time interval between every two adjacent target microscopic images in the image sequence; based on a proper time interval, the user can effectively observe the variation trend of the sample along with the temperature. Wherein in some embodiments the time interval is preset, and in other embodiments the time interval is user-specifiable. Wherein in some embodiments, the method further comprises step S400 (not shown); in step S400, the microscope system acquires a time interval between every two adjacent target microscope images in the image sequence. For example, when the time interval is determined based on a preset, in some embodiments the microscope system reads the corresponding profile to determine the time interval; when the time interval is designated by the user, the microscope system then detects the user's associated action to determine the time interval.
Of course, in order to meet the requirement of a user for observing the change trend of the sample along with the temperature, the instant temperatures can be arranged firstly, a time interval is set, the instant temperature corresponding to the head of the queue of the instant temperatures is used as the current instant temperature, and the current instant temperature is updated to be the next instant temperature in the queue in sequence based on the time interval; and simultaneously, sequentially presenting the target microscopic images corresponding to the current instant temperature. Accordingly, in some embodiments, the microscope system sequentially presents the plurality of target microscope images based on a time interval between each two adjacent instantaneous temperatures in the temperature sequence.
In some embodiments, to facilitate a researcher viewing a sample at varying angles to obtain more detail of the sample as a function of temperature, the target microscopic image includes information on the spatial location of points on the sample that are acquired; accordingly, in the above step S300, the microscope system sequentially presents the plurality of target microscope images based on the temperature sequence and the presentation pose information of each target microscope image. For example, based on a spatial position, a pose specified by a user, the microscope system renders a target microscopic image on its display device (e.g., display screen). In particular, in some embodiments, the pixel parameters of each pixel of each target microscopic image include spatial location information of the respective pixel; accordingly, in step S300, the microscope system sequentially presents the plurality of target microscope images according to the spatial position information of the corresponding pixels in each target microscope image based on the temperature sequence and the presentation pose information of each target microscope image. For example, when the microscope system takes an image of the sample for each instant temperature, the spatial position of the corresponding point on the sample is also recorded.
Referring to FIG. 3, one implementation of obtaining the spatial location of each point on the specimen is based on the focal plane height of the imaging system at the time the point image was taken. Under the condition of large magnification, the depth of field of the imaging system is small, so that under the condition that a sample has certain unevenness, in order to clearly image different parts of the sample respectively, the focal plane height needs to be changed aiming at the parts with different heights on the sample; thus, the current focal plane height at which the sample can be imaged sharply corresponds to the spatial height (z direction) of the sample being imaged sharply, and the height difference between different focal plane heights reflects the height difference (h) of different parts of the samplex). In the horizontal direction, for the same imaging field of view, different points are in one-to-one correspondence with the positions of the imaging on the imaging element, so that the positions of the corresponding points on the sample in the space can be known based on the current position of the imaging element and the position of the image on the imaging element. For example, in the case where the position of the imaging element is known, a mapping table may be established in advance based on the mapping relationship between each point (or pixel) on the imaging element and each corresponding point (the horizontal positions of all points) on the calibration object, and then the actual sample is imaged, so that the horizontal positions of each point on the sample are found based on the mapping table and the imaging positions of each point on the imaging element; at the same time, the vertical position of the corresponding point on the specimen is determined based on the focal plane height of the objective lens, thereby determining the spatial position of the corresponding point on the specimen.
It should be understood that the above-described method for obtaining the spatial position of a point on a sample based on imaging is only an example and not a limitation of the present embodiment, and other existing or later-developed embodiments may be applied to the present application and are included in the scope of the present application and are incorporated by reference herein. For example, the spatial (horizontal) position of a corresponding point on the specimen is directly calculated based on the pinhole imaging model and the imaging position of each point on the imaging element.
When the shot image is presented, each pixel is presented in a screen coordinate system of the display device according to the spatial position of the pixel, and the pixel value is determined based on the imaging information of the imaging element. Thus, after the target microscopic image is presented, the color information of each point on the sample can be provided to the user, and the fluctuation state of the sample surface can also be provided to the user.
In order to facilitate the user to adjust the posture of the sample so as to observe the temperature variation of the corresponding portion of the sample from a desired angle according to actual requirements, in some embodiments, the step S300 includes a sub-step S330 and a sub-step S340 (both not shown). Wherein in step S330, the microscope system acquires real-time rendering pose information (e.g., real-time position, pitch, etc. position, pose information relative to a screen coordinate system) of the plurality of target microscope images; in step S340, the microscope system sequentially presents the plurality of target microscopic images based on the temperature sequence and the real-time presentation pose information, for example, the target microscopic images are all presented based on the same real-time presentation pose information. In some embodiments, the content presented on the screen of the microscope system is a projection of the target microscope image based on the screen coordinate system. In particular, the system provides an initial pose of the target microscopic image, and then the user can adjust (or correct) the viewing angle, position, etc. based on the pose to meet the actual requirement; accordingly, in the above step S330, the microscope system acquires initial presentation pose information of the plurality of target microscope images, determines pose correction information of the plurality of target microscope images in response to a pose correction operation by a user, and determines real-time presentation pose information of the plurality of target microscope images based on the initial presentation pose information and the pose correction information.
In some embodiments, in the step S300, the microscope system generates a microscopic video based on the temperature sequence, and plays the microscopic video, wherein at least one video frame in the microscopic video is generated based on the target microscopic image. The generated microscopic video can be played again at any time and is convenient to be provided to other users for watching, such as teaching. At the moment, equipment used by other users watching the microscopic video only needs to have the playing function of the corresponding video, so that the communication cost is greatly reduced.
In addition to providing the user with a trend of the sample as a function of temperature, to facilitate a comparison of the currently presented target microscopic image and the corresponding specific temperature, in some embodiments, the microscope system sequentially presents the plurality of target microscopic images and the corresponding instantaneous temperatures based on the temperature sequence.
In addition, in order to facilitate the data storage and the communication between users (for example, to enable users without microscope devices to participate in research), the target microscopic image is stored in a network device (for example, a cloud server). Accordingly, in some embodiments, in step S100, the microscope system sends an image request to the corresponding network device to obtain a plurality of target microscopic images about the target specimen, wherein each target microscopic image corresponds to an instant temperature. For example, the microscope system receives a series of target microscopic images returned by the network device based on the image request and instant temperature information corresponding to the target microscopic images. In some embodiments, the microscope system sends an image request to a corresponding network device to obtain a plurality of target microscopic images of a target sample, wherein the image request includes a plurality of instant temperatures, and each target microscopic image corresponds to one of the instant temperatures respectively, so as to reduce the operation burden of a user. For example, the instantaneous temperature corresponding to the image that the user needs to study, which in some cases is determined by the computer according to the temperature range specified by the user; in particular, the temperatures are determined by the computer based on the temperature range and an adjustable temperature step (e.g., acquiring microscopic images of the target every few degrees celsius).
According to another aspect of the present application, a microscope system for presenting a microscopic image of an object is provided. Referring to fig. 4, the system includes a first module 100, a second module 200, and a third module 300. The first module 100, the second module 200, and the third module 300 are respectively configured to perform the operations in the steps S100, S200, and S300, and for a specific implementation, please refer to related embodiments of the steps S100, S200, and S300, which are not described herein again.
The present application also provides a computer readable storage medium having stored thereon computer code which, when executed, performs a method as in any one of the preceding.
The present application also provides a computer program product, which when executed by a computer device, performs the method of any of the preceding claims.
The present application further provides a computer device, comprising:
one or more processors;
a memory for storing one or more computer programs;
the one or more computer programs, when executed by the one or more processors, cause the one or more processors to implement the method of any preceding claim.
FIG. 5 illustrates an exemplary system that can be used to implement the various embodiments described in this application.
As shown, in some embodiments, the system 1000 can function as any one of the microscope device/computer device/microscope system in each of the described embodiments. In some embodiments, system 1000 may include one or more computer-readable media (e.g., system memory or NVM/storage 1020) having instructions and one or more processors (e.g., processor(s) 1005) coupled with the one or more computer-readable media and configured to execute the instructions to implement modules to perform the actions described herein.
For one embodiment, system control module 1010 may include any suitable interface controllers to provide any suitable interface to at least one of the processor(s) 1005 and/or to any suitable device or component in communication with system control module 1010.
The system control module 1010 may include a memory controller module 1030 to provide an interface to the system memory 1015. Memory controller module 1030 may be a hardware module, a software module, and/or a firmware module.
System memory 1015 may be used to load and store data and/or instructions, for example, for system 1000. For one embodiment, system memory 1015 may include any suitable volatile memory, such as suitable DRAM. In some embodiments, system memory 1015 may include double data rate type four synchronous dynamic random access memory (DDR4 SDRAM).
For one embodiment, system control module 1010 may include one or more input/output (I/O) controllers to provide an interface to NVM/storage 1020 and communication interface(s) 1025.
For example, NVM/storage 1020 may be used to store data and/or instructions. NVM/storage 1020 may include any suitable non-volatile memory (e.g., flash memory) and/or may include any suitable non-volatile storage device(s) (e.g., one or more Hard Disk drive(s) (HDD (s)), one or more Compact Disc (CD) drive(s), and/or one or more Digital Versatile Disc (DVD) drive (s)).
NVM/storage 1020 may include storage resources that are physically part of a device on which system 1000 is installed or may be accessed by the device and not necessarily part of the device. For example, NVM/storage 1020 may be accessed over a network via communication interface(s) 1025.
Communication interface(s) 1025 may provide an interface for system 1000 to communicate over one or more networks and/or with any other suitable device. System 1000 may communicate wirelessly with one or more components of a wireless network according to any of one or more wireless network standards and/or protocols.
For one embodiment, at least one of the processor(s) 1005 may be packaged together with logic for one or more controller(s) of the system control module 1010, e.g., memory controller module 1030. For one embodiment, at least one of the processor(s) 1005 may be packaged together with logic for one or more controller(s) of the system control module 1010 to form a System In Package (SiP). For one embodiment, at least one of the processor(s) 1005 may be integrated on the same die with logic for one or more controller(s) of the system control module 1010. For one embodiment, at least one of the processor(s) 1005 may be integrated on the same die with logic of one or more controllers of the system control module 1010 to form a system on a chip (SoC).
In various embodiments, system 1000 may be, but is not limited to being: a server, a workstation, a desktop computing device, or a mobile computing device (e.g., a laptop computing device, a handheld computing device, a tablet, a netbook, etc.). In various embodiments, system 1000 may have more or fewer components and/or different architectures. For example, in some embodiments, system 1000 includes one or more cameras, a keyboard, a Liquid Crystal Display (LCD) screen (including a touch screen display), a non-volatile memory port, multiple antennas, a graphics chip, an Application Specific Integrated Circuit (ASIC), and speakers.
It should be noted that the present application may be implemented in software and/or a combination of software and hardware, for example, implemented using Application Specific Integrated Circuits (ASICs), general purpose computers or any other similar hardware devices. In one embodiment, the software programs of the present application may be executed by a processor to implement the steps or functions described above. Likewise, the software programs (including associated data structures) of the present application may be stored in a computer readable recording medium, such as RAM memory, magnetic or optical drive or diskette and the like. Additionally, some of the steps or functions of the present application may be implemented in hardware, for example, as circuitry that cooperates with the processor to perform various steps or functions.
In addition, some of the present application may be implemented as a computer program product, such as computer program instructions, which when executed by a computer, may invoke or provide methods and/or techniques in accordance with the present application through the operation of the computer. Those skilled in the art will appreciate that the form in which the computer program instructions reside on a computer-readable medium includes, but is not limited to, source files, executable files, installation package files, and the like, and that the manner in which the computer program instructions are executed by a computer includes, but is not limited to: the computer directly executes the instruction, or the computer compiles the instruction and then executes the corresponding compiled program, or the computer reads and executes the instruction, or the computer reads and installs the instruction and then executes the corresponding installed program. Computer-readable media herein can be any available computer-readable storage media or communication media that can be accessed by a computer.
Communication media includes media by which communication signals, including, for example, computer readable instructions, data structures, program modules, or other data, are transmitted from one system to another. Communication media may include conductive transmission media such as cables and wires (e.g., fiber optics, coaxial, etc.) and wireless (non-conductive transmission) media capable of propagating energy waves such as acoustic, electromagnetic, RF, microwave, and infrared. Computer readable instructions, data structures, program modules, or other data may be embodied in a modulated data signal, for example, in a wireless medium such as a carrier wave or similar mechanism such as is embodied as part of spread spectrum techniques. The term "modulated data signal" means a signal that has one or more of its characteristics set or changed in such a manner as to encode information in the signal. The modulation may be analog, digital or hybrid modulation techniques.
By way of example, and not limitation, computer-readable storage media may include volatile and nonvolatile, removable and non-removable media implemented in any method or technology for storage of information such as computer-readable instructions, data structures, program modules or other data. For example, computer-readable storage media include, but are not limited to, volatile memory such as random access memory (RAM, DRAM, SRAM); and non-volatile memory such as flash memory, various read-only memories (ROM, PROM, EPROM, EEPROM), magnetic and ferromagnetic/ferroelectric memories (MRAM, FeRAM); and magnetic and optical storage devices (hard disk, tape, CD, DVD); or other now known media or later developed that can store computer-readable information/data for use by a computer system.
An embodiment according to the present application comprises an apparatus comprising a memory for storing computer program instructions and a processor for executing the program instructions, wherein the computer program instructions, when executed by the processor, trigger the apparatus to perform a method and/or a solution according to the aforementioned embodiments of the present application.
It will be evident to those skilled in the art that the present application is not limited to the details of the foregoing illustrative embodiments, and that the present application may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned. Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. A plurality of units or means recited in the apparatus claims may also be implemented by one unit or means in software or hardware. The terms first, second, etc. are used to denote names, but not any particular order.
Claims (18)
1. A method for presenting a microscopic image of an object, wherein the method comprises:
acquiring a plurality of target microscopic images related to a target sample, wherein each target microscopic image corresponds to an instant temperature respectively;
generating a temperature sequence based on each instant temperature;
sequentially presenting the plurality of target microscopic images based on the temperature sequence.
2. The method of claim 1, wherein the generating a temperature sequence based on the instantaneous temperatures comprises:
and arranging the instant temperatures according to a preset temperature arrangement rule to generate a corresponding temperature sequence.
3. The method of claim 1, wherein the generating a temperature sequence based on the instantaneous temperatures comprises:
and responding to the temperature arrangement operation of the user on each instant temperature to generate a corresponding temperature sequence.
4. The method of claim 1, wherein the presenting the plurality of target microscopic images based on the temperature sequence order comprises:
generating respective image sequences for the plurality of target microscopic images based on the temperature sequences;
sequentially presenting the plurality of target microscopic images based on the sequence of images.
5. The method of claim 4, wherein the generating respective image sequences for the plurality of target microscopic images based on the temperature sequence comprises:
and generating an image sequence corresponding to the temperature sequence based on the corresponding relation between each instant temperature and the corresponding target microscopic image.
6. The method of claim 4, wherein the sequentially presenting the plurality of target microscopic images based on the sequence of images comprises:
sequentially presenting the plurality of target microscopic images based on a time interval between each two adjacent target microscopic images in the image sequence.
7. The method of claim 6, wherein the method further comprises:
and acquiring the time interval between every two adjacent target microscopic images in the image sequence.
8. The method of claim 1, wherein the presenting the plurality of target microscopic images based on the temperature sequence order comprises:
and sequentially presenting the plurality of target microscopic images based on the time interval between every two adjacent instantaneous temperatures in the temperature sequence.
9. The method of claim 1, wherein the presenting the plurality of target microscopic images based on the temperature sequence order comprises:
and sequentially presenting the plurality of target microscopic images based on the temperature sequence and the presentation pose information of each target microscopic image.
10. The method of claim 9, wherein the pixel parameters of each pixel of each target microscopic image include spatial location information of the respective pixel;
sequentially presenting the plurality of target microscopic images based on the temperature sequence and the presentation pose information of each target microscopic image, including:
and sequentially presenting the plurality of target microscopic images according to the spatial position information of the corresponding pixels in each target microscopic image based on the temperature sequence and the presentation pose information of each target microscopic image.
11. The method of claim 9, wherein the sequentially presenting the plurality of target microscopy images based on the temperature sequence and the presentation pose information of each target microscopy image comprises:
acquiring real-time presenting pose information of the plurality of target microscopic images;
and sequentially presenting the plurality of target microscopic images based on the temperature sequence and the real-time pose information.
12. The method of claim 11, wherein the acquiring real-time pose information of the plurality of target microscopic images comprises:
acquiring initial presenting pose information of the plurality of target microscopic images;
determining pose correction information of the plurality of target microscopic images in response to a pose correction operation of a user;
and determining real-time presenting pose information of the plurality of target microscopic images based on the initial presenting pose information and the pose correction information.
13. The method of claim 1, wherein the presenting the plurality of target microscopic images based on the temperature sequence order comprises:
generating a microscopy video based on the temperature sequence, wherein at least one video frame in the microscopy video is generated based on the target microscopy image;
and playing the microscopic video.
14. The method of claim 1, wherein the presenting the plurality of target microscopic images based on the temperature sequence order comprises:
sequentially presenting the plurality of target microscopic images and corresponding instantaneous temperatures based on the temperature sequence.
15. The method of claim 1, wherein said acquiring a plurality of target microscopic images about a target specimen, wherein each target microscopic image corresponds to an instantaneous temperature, comprises:
and sending an image request to the corresponding network equipment to acquire a plurality of target microscopic images about the target sample, wherein each target microscopic image corresponds to an instant temperature.
16. The method of claim 15, wherein said sending an image request to a corresponding network device to obtain a plurality of target microscopic images about a target specimen, wherein each target microscopic image corresponds to an instantaneous temperature, respectively, comprises:
sending an image request to a corresponding network device to obtain a plurality of target microscopic images about a target specimen, wherein the image request includes a plurality of instantaneous temperatures, and each target microscopic image corresponds to one of the instantaneous temperatures.
17. A microscope system for presenting a microscopic image of an object, wherein the microscope system comprises:
a processor; and
a memory arranged to store computer-executable instructions that, when executed, cause the processor to perform operations according to the method of any one of claims 1 to 16.
18. A computer-readable medium storing instructions that, when executed by a computer, cause the computer to perform operations according to the method of any one of claims 1 to 16.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007278896A (en) * | 2006-04-07 | 2007-10-25 | Olympus Corp | Scanning probe microscope and method for acquiring image reflecting physical property value of specimen |
| CN101231249A (en) * | 2008-02-26 | 2008-07-30 | 东北大学 | Vacuum low temperature microscopic visualizer |
| US20090016407A1 (en) * | 2007-07-09 | 2009-01-15 | Kabushiki Kaisha Kobe Seiko Sho | Temperature-measuring member, temperature-measuring device, and method for measuring temperature |
| CN101487806A (en) * | 2009-02-20 | 2009-07-22 | 中山大学 | DSC meter with visualization function |
| CN102841079A (en) * | 2011-06-22 | 2012-12-26 | 徕卡显微系统复合显微镜有限公司 | Method and light-microscopic device to show a sample as an image |
| CN104391371A (en) * | 2014-12-19 | 2015-03-04 | 成都理工大学 | Digital microscope and use method thereof |
| CN106093047A (en) * | 2016-07-25 | 2016-11-09 | 武汉大学 | A kind of soil body biological activity develops pattern recognition device and the method for monitoring in real time |
| CN106226243A (en) * | 2016-08-02 | 2016-12-14 | 大连理工大学 | A kind of utilize the method for cooling procedure and its condensed state structure relation in thermally indicating material observation Injection moulded part mould |
| CN109612998A (en) * | 2019-01-30 | 2019-04-12 | 重庆大学 | A kind of micro-manipulation device and its control method of multifactor collaboration |
-
2020
- 2020-03-12 CN CN202010172202.XA patent/CN113390868A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007278896A (en) * | 2006-04-07 | 2007-10-25 | Olympus Corp | Scanning probe microscope and method for acquiring image reflecting physical property value of specimen |
| US20090016407A1 (en) * | 2007-07-09 | 2009-01-15 | Kabushiki Kaisha Kobe Seiko Sho | Temperature-measuring member, temperature-measuring device, and method for measuring temperature |
| CN101231249A (en) * | 2008-02-26 | 2008-07-30 | 东北大学 | Vacuum low temperature microscopic visualizer |
| CN101487806A (en) * | 2009-02-20 | 2009-07-22 | 中山大学 | DSC meter with visualization function |
| CN102841079A (en) * | 2011-06-22 | 2012-12-26 | 徕卡显微系统复合显微镜有限公司 | Method and light-microscopic device to show a sample as an image |
| CN104391371A (en) * | 2014-12-19 | 2015-03-04 | 成都理工大学 | Digital microscope and use method thereof |
| CN106093047A (en) * | 2016-07-25 | 2016-11-09 | 武汉大学 | A kind of soil body biological activity develops pattern recognition device and the method for monitoring in real time |
| CN106226243A (en) * | 2016-08-02 | 2016-12-14 | 大连理工大学 | A kind of utilize the method for cooling procedure and its condensed state structure relation in thermally indicating material observation Injection moulded part mould |
| CN109612998A (en) * | 2019-01-30 | 2019-04-12 | 重庆大学 | A kind of micro-manipulation device and its control method of multifactor collaboration |
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Application publication date: 20210914 |