CN113358455A - Method for automatically and rapidly digesting food sample - Google Patents
Method for automatically and rapidly digesting food sample Download PDFInfo
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- CN113358455A CN113358455A CN202110522524.7A CN202110522524A CN113358455A CN 113358455 A CN113358455 A CN 113358455A CN 202110522524 A CN202110522524 A CN 202110522524A CN 113358455 A CN113358455 A CN 113358455A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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Abstract
The invention discloses a method for automatically and rapidly digesting a food sample, and relates to the technical field of food digestion. The invention comprises the steps of sealing, mounting, microwave digestion and constant volume test, wherein the sealing comprises the following steps: the method comprises the following steps: preparing a clean master control tank sample dissolving cup, wherein the volume of the sample dissolving cup is 1L; step two: weighing 0.5g of food sample, and placing the food sample in a sample dissolving cup; step three: adding 6ml of nitric acid into the microwave digestion tank, wherein the concentration of the nitric acid is 65-68%; step four: and covering the tank cover on the top of the digestion tank, and sealing the digestion tank. According to the microwave oven, the digestion time of food can be shortened by heating the microwave oven, the digestion amount can be increased, a large amount of food can be digested, the digestion efficiency of the food is improved, the heating temperature and the heating time are set for the microwave oven, the heating temperature and the heating time can be controlled and adjusted when the food is digested, and the application range and the practicability of the digestion method are improved.
Description
Technical Field
The invention belongs to the technical field of food digestion, and particularly relates to a method for automatically and rapidly digesting a food sample.
Background
Food needs to be digested during inspection, however, the existing food digestion has certain defects in use, the digestion time is long, the digestion amount is small, and meanwhile, when the food is digested, the heating temperature and the heating time are uncontrollable, and the use range is limited.
Disclosure of Invention
The invention aims to provide a method for automatically and quickly digesting a food sample, which solves the existing problems that: the existing food digestion is usually long in digestion time and small in digestion amount, and meanwhile, digestion liquid needs to be prepared manually, so that the labor intensity of operators is increased.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a method for automatically and rapidly digesting a food sample, which comprises the following steps of sealing, installing, microwave digestion and constant volume test, wherein the sealing comprises the following steps:
the method comprises the following steps: preparing a clean master control tank sample dissolving cup, wherein the volume of the sample dissolving cup is 1L;
step two: weighing 0.5g of food sample, and placing the food sample in a sample dissolving cup;
step three: adding 6ml of nitric acid into the microwave digestion tank, wherein the concentration of the nitric acid is 65-68%;
step four: and covering the tank cover on the top of the digestion tank, and sealing the digestion tank.
Further, the mounting comprises the steps of:
the method comprises the following steps: preparing a parallel sample and a blank sample according to the method;
step two: the parallel sample and the blank sample both contain the same amount of reagent and 0.5ml of secondary distilled water or deionized water, and the blank sample is not used as a main control tank;
step three: fixing the main control tank, and installing a temperature sensor on the outer surface of the main control tank;
step four: and uniformly placing the rest of the tank bodies into a seat frame of the turntable, and fixing the rest of the tank bodies.
Further, the microwave digestion comprises the following steps:
the method comprises the following steps: placing the main control tank and the digestion tank in a microwave oven;
step two: digesting the food in the main control tank by selecting a microwave temperature control mode;
step three: heating the microwave oven to 100 ℃ for 5 minutes;
step four: the microwave oven was heated to 160 ℃ for a 15 minute run time.
Further, the constant volume test comprises the following steps:
the method comprises the following steps: after digestion is finished, closing the microwave oven, cooling the main control tank and the digestion tank in the microwave oven to be within 90 ℃, sequentially taking out the main control tank and the digestion tank, and dismounting the temperature sensor from the outer side surface of the main control tank;
step two: when the temperature of the jacket is cooled to room temperature, the air escape valve is rotated in the fume hood, and the pressure of the fume hood is released;
step three: opening a sample dissolving cup in the main control tank, and transferring the sample digestion solution into a 70ml volumetric flask;
step four: the solution obtained after cleaning the inner wall of the sample dissolving cup and the cup cover is rotated into a volumetric flask by deionized water or secondary distilled water, and the volume is fixed to 70 ml;
step five: the contents of the volumetric flask were then tested using a digestion apparatus.
The invention has the following beneficial effects:
1. the microwave oven is arranged in the microwave oven for heating, so that the digestion time of food can be reduced, the digestion amount can be increased, a large amount of food can be digested, and the digestion efficiency of the food is improved.
2. According to the invention, the heating temperature and the heating time are set for the microwave oven, so that the heating temperature and the heating time can be controlled and adjusted when the food is digested, and the application range and the practicability of the digestion method are improved.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Detailed Description
Technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention relates to a method for automatically and rapidly digesting a food sample, which comprises the following steps of sealing, mounting, microwave digestion and constant volume test, wherein the sealing comprises the following steps:
the method comprises the following steps: preparing a clean master control tank sample dissolving cup, wherein the volume of the sample dissolving cup is 1L;
step two: weighing 0.5g of food sample, and placing the food sample in a sample dissolving cup;
step three: adding 6ml of nitric acid into the microwave digestion tank, wherein the concentration of the nitric acid is 65-68%;
step four: and covering the tank cover on the top of the digestion tank, and sealing the digestion tank.
The installation comprises the following steps:
the method comprises the following steps: preparing a parallel sample and a blank sample according to the method;
step two: the parallel sample and the blank sample both contain the same amount of reagent and 0.5ml of secondary distilled water or deionized water, and the blank sample is not used as a main control tank;
step three: fixing the main control tank, and installing a temperature sensor on the outer surface of the main control tank;
step four: and uniformly placing the rest of the tank bodies into a seat frame of the turntable, and fixing the rest of the tank bodies.
The microwave digestion comprises the following steps:
the method comprises the following steps: placing the main control tank and the digestion tank in a microwave oven;
step two: digesting the food in the main control tank by selecting a microwave temperature control mode;
step three: heating the microwave oven to 100 ℃ for 5 minutes;
step four: the microwave oven was heated to 160 ℃ for a 15 minute run time.
The constant volume test comprises the following steps:
the method comprises the following steps: after digestion is finished, closing the microwave oven, cooling the main control tank and the digestion tank in the microwave oven to be within 90 ℃, sequentially taking out the main control tank and the digestion tank, and dismounting the temperature sensor from the outer side surface of the main control tank;
step two: when the temperature of the jacket is cooled to room temperature, the air escape valve is rotated in the fume hood, and the pressure of the fume hood is released;
step three: opening a sample dissolving cup in the main control tank, and transferring the sample digestion solution into a 70ml volumetric flask;
step four: the solution obtained after cleaning the inner wall of the sample dissolving cup and the cup cover is rotated into a volumetric flask by deionized water or secondary distilled water, and the volume is fixed to 70 ml;
step five: the contents of the volumetric flask were then tested using a digestion apparatus.
One specific application of this embodiment is: preparing a clean master control tank sample dissolving cup, wherein the volume of the sample dissolving cup is 1L, weighing 0.5g of food sample, placing the food sample in the sample dissolving cup, adding 6ml of nitric acid into a microwave digestion tank, the concentration of the nitric acid is 65-68%, covering a tank cover on the top of the digestion tank, sealing the digestion tank, preparing a parallel sample and a blank sample according to the method, wherein the parallel sample and the blank sample both contain the same amount of reagents and 0.5ml of secondary distilled water or deionized water, the blank sample is not used as the master control tank, fixing the master control tank, installing a temperature sensor on the outer surface of the master control tank, uniformly placing the rest tank bodies in a seat frame of a turntable, fixing the rest tank bodies, placing the master control tank and the blank tank in a microwave oven, digesting the food in the master control tank by selecting a microwave temperature control mode, heating the temperature of the microwave oven to 100 ℃, the running time is 5 minutes, then the temperature of the microwave oven is heated to 160 ℃, the running time is 15 minutes, the microwave oven is closed after digestion is completed, the main control tank and the digestion tank are cooled to be within 90 ℃ in the microwave oven, the main control tank and the digestion tank are sequentially taken out, the temperature sensor is dismounted from the outer side surface of the main control tank, when the temperature of the outer sleeve is cooled to be room temperature, the air release valve is rotated in the ventilation cabinet, the pressure of the ventilation cabinet is released, the sample dissolving cup in the main control tank is opened, the sample digestion solution is transferred into a volumetric flask with 70ml, the solution obtained after the inner wall of the sample dissolving cup and the cup cover are cleaned is rotated into the volumetric flask by deionized water or secondary distilled water, the volume is fixed to 70ml, and then the digestion instrument is used for testing the inside of the volumetric flask.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (4)
1. The method for automatically and rapidly digesting the food sample comprises the steps of sealing, installing, microwave digestion and constant volume test, and is characterized in that: the sealing comprises the following steps:
the method comprises the following steps: preparing a clean master control tank sample dissolving cup, wherein the volume of the sample dissolving cup is 1L;
step two: weighing 0.5g of food sample, and placing the food sample in a sample dissolving cup;
step three: adding 6ml of nitric acid into the microwave digestion tank, wherein the concentration of the nitric acid is 65-68%;
step four: and covering the tank cover on the top of the digestion tank, and sealing the digestion tank.
2. The method for automatically and rapidly digesting the food sample according to claim 1, is characterized in that: the installation comprises the following steps:
the method comprises the following steps: preparing a parallel sample and a blank sample according to the method;
step two: the parallel sample and the blank sample both contain the same amount of reagent and 0.5ml of secondary distilled water or deionized water, and the blank sample is not used as a main control tank;
step three: fixing the main control tank, and installing a temperature sensor on the outer surface of the main control tank;
step four: and uniformly placing the rest of the tank bodies into a seat frame of the turntable, and fixing the rest of the tank bodies.
3. The method for automatically and rapidly digesting the food sample according to claim 1, is characterized in that: the microwave digestion comprises the following steps:
the method comprises the following steps: placing the main control tank and the digestion tank in a microwave oven;
step two: digesting the food in the main control tank by selecting a microwave temperature control mode;
step three: heating the microwave oven to 100 ℃ for 5 minutes;
step four: the microwave oven was heated to 160 ℃ for a 15 minute run time.
4. The method for automatically and rapidly digesting the food sample according to claim 1, is characterized in that: the constant volume test comprises the following steps:
the method comprises the following steps: after digestion is finished, closing the microwave oven, cooling the main control tank and the digestion tank in the microwave oven to be within 90 ℃, sequentially taking out the main control tank and the digestion tank, and dismounting the temperature sensor from the outer side surface of the main control tank;
step two: when the temperature of the jacket is cooled to room temperature, the air escape valve is rotated in the fume hood, and the pressure of the fume hood is released;
step three: opening a sample dissolving cup in the main control tank, and transferring the sample digestion solution into a 70ml volumetric flask;
step four: the solution obtained after cleaning the inner wall of the sample dissolving cup and the cup cover is rotated into a volumetric flask by deionized water or secondary distilled water, and the volume is fixed to 70 ml;
step five: the contents of the volumetric flask were then tested using a digestion apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110522524.7A CN113358455A (en) | 2021-05-13 | 2021-05-13 | Method for automatically and rapidly digesting food sample |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110522524.7A CN113358455A (en) | 2021-05-13 | 2021-05-13 | Method for automatically and rapidly digesting food sample |
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| CN113358455A true CN113358455A (en) | 2021-09-07 |
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| CN202110522524.7A Pending CN113358455A (en) | 2021-05-13 | 2021-05-13 | Method for automatically and rapidly digesting food sample |
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| CN102519939A (en) * | 2011-11-28 | 2012-06-27 | 沈阳工业大学 | Quantitative analytical method for determining trace cobalt in water by utilizing microwave digestion flame atomic absorption spectrometry (FAAS) method |
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| CN104730013A (en) * | 2015-03-25 | 2015-06-24 | 广东出入境检验检疫局检验检疫技术中心 | Method for judging effective life of graphite pipe in graphite furnace atomic absorption spectrometer |
| CN104949960A (en) * | 2014-03-25 | 2015-09-30 | 沈阳师范大学 | Method for measuring content of heavy metals in edible mushrooms by ICP-AES (inductively coupled plasma-atomic emission spectrometry) |
| CN106918478A (en) * | 2015-12-24 | 2017-07-04 | 青岛清泉生物科技有限公司 | The pre-treating method of sea-tangle determining heavy metals |
| CN110954589A (en) * | 2018-09-26 | 2020-04-03 | 广州金域医学检验中心有限公司 | Method for measuring arsenic in food additive by ICP-MS (inductively coupled plasma-mass spectrometry) |
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2021
- 2021-05-13 CN CN202110522524.7A patent/CN113358455A/en active Pending
Patent Citations (7)
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| CN1442694A (en) * | 2003-04-11 | 2003-09-17 | 济南市疾病预防控制中心 | Method of decomposing food sample using micro wave |
| CN102519939A (en) * | 2011-11-28 | 2012-06-27 | 沈阳工业大学 | Quantitative analytical method for determining trace cobalt in water by utilizing microwave digestion flame atomic absorption spectrometry (FAAS) method |
| CN104949960A (en) * | 2014-03-25 | 2015-09-30 | 沈阳师范大学 | Method for measuring content of heavy metals in edible mushrooms by ICP-AES (inductively coupled plasma-atomic emission spectrometry) |
| CN104215627A (en) * | 2014-05-09 | 2014-12-17 | 武汉红金龙印务股份有限公司 | Method for microwave digestion-inductively coupled plasma mass spectrometer (ICP-MS) determination of metal ions such as lead, arsenic, cadmium and chromium in cigarette case |
| CN104730013A (en) * | 2015-03-25 | 2015-06-24 | 广东出入境检验检疫局检验检疫技术中心 | Method for judging effective life of graphite pipe in graphite furnace atomic absorption spectrometer |
| CN106918478A (en) * | 2015-12-24 | 2017-07-04 | 青岛清泉生物科技有限公司 | The pre-treating method of sea-tangle determining heavy metals |
| CN110954589A (en) * | 2018-09-26 | 2020-04-03 | 广州金域医学检验中心有限公司 | Method for measuring arsenic in food additive by ICP-MS (inductively coupled plasma-mass spectrometry) |
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