CN113354698A - Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant - Google Patents
Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant Download PDFInfo
- Publication number
- CN113354698A CN113354698A CN202110630095.5A CN202110630095A CN113354698A CN 113354698 A CN113354698 A CN 113354698A CN 202110630095 A CN202110630095 A CN 202110630095A CN 113354698 A CN113354698 A CN 113354698A
- Authority
- CN
- China
- Prior art keywords
- acetylglucosamine
- flocculant
- fermentation
- liquid
- flocculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
- C07H5/04—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to nitrogen
- C07H5/06—Aminosugars
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention relates to a method for preparing N-acetamido sugar high-density fermentation clear liquid by using a composite flocculant. Belongs to the field of bioengineering, and comprises the following steps: inactivating the N-acetylglucosamine fermentation liquor; adding an inorganic flocculant, and stirring to obtain N-acetylglucosamine fermentation liquor containing the inorganic flocculant; adding adsorbent and filter aid, stirring, adding the prepared organic polymeric flocculant, stirring uniformly, standing for flocculation for 30-60min to obtain a flocculation liquid of the N-acetylglucosamine fermentation liquid; and performing plate-and-frame filter pressing to obtain the N-acetylglucosamine fermentation clear liquid. The flocculation impurity removal process has obvious effect, clear liquid yield is high after plate-frame filtration, the water content of the mushroom dregs is low, and the consumption of a flocculating agent is reduced by composite flocculation. The process is simple to operate, has no three-waste discharge, and is an environment-friendly, simple and efficient method for preparing the acetamido sugar fermentation clear liquid.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to a method for preparing N-acetylglucosamine fermentation clear liquid by using a composite flocculant.
Background
N-acetylglucosamine has the chemical formula of C8H15NO6. Is white powder, is easily soluble in water, and is important polysaccharide in biological cellThe fundamental constituent units, especially crustaceans, have the highest exoskeleton content. It is an important precursor for the synthesis of bifidus factors and has many important physiological functions in the organism. For example, as a monomer for chitin. Has anti-inflammatory, anti-tumor and antioxidant effects, can be used for treating osteoarthritis and rheumatic arthritis clinically, can also be used as food antioxidant and sweetener of infant food additive for diabetic patients, and has wide application in fields of food, medicine, cosmetics, etc.
With the rapid development of genetic engineering and synthetic biology, the recombinant microorganism can directly utilize glucose to synthesize GlcNAc, the product concentration can even exceed 140g/L, and the production scale and production level of N-acetylglucosamine and glucosamine salts are greatly improved. The invention is the first step of developing a complete set of process for producing N-acetyl glucosamine by high-density fermentation of microorganisms, namely the preparation of fermentation clear liquid, and lays a solid foundation for the preparation of N-acetyl glucosamine and glucosamine salts.
The N-acetylglucosamine high-density fermentation liquor has high bacterial content and high viscosity, the membrane filtration concentration factor is low, a large amount of pure water is needed to wash out the product N-acetylglucosamine in a concentrated phase, the volume of clear liquid is greatly increased, the concentration of the product is reduced, the next procedure can be smoothly carried out only by re-concentration, and the cost is high. Similarly, because the engineering bacteria high-density fermentation bacteria have large quantity and small bacteria and can be filtered by a plate frame after flocculation, and the actual research process finds that the single inorganic flocculant or organic flocculant can not flocculate the bacteria to achieve perfect plate frame filtration, the company develops an additive inorganic and organic composite flocculant to enable the high-density bacteria to be flocculated perfectly. The volume of the filtered clear liquid is not increased, and the thalli can be removed through plate-and-frame filter pressing smoothly to prepare the high-concentration acetylglucosamine clear liquid.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to provide a method for preparing N-acetylglucosamine fermentation clear liquid by using a composite flocculant.
The technical scheme is as follows: the invention relates to a method for preparing N-acetylglucosamine fermentation clear liquid by using a composite flocculant, which comprises the following specific operation steps:
(1.1) inactivating the N-acetylglucosamine fermentation liquor produced by high-density fermentation of large intestine engineering bacteria;
(1.2) adding an inorganic flocculant into the inactivated N-acetylglucosamine fermentation liquor, and uniformly stirring to obtain the N-acetylglucosamine fermentation liquor containing the inorganic flocculant;
(1.3) adding an adsorbent and a filter aid into the N-acetylglucosamine fermentation liquor containing the inorganic flocculant, uniformly stirring, adding the prepared organic polymeric flocculant, uniformly stirring, standing for flocculation for 30-60min, and thus obtaining a flocculation liquid of the N-acetylglucosamine fermentation liquor;
(1.4) finally, carrying out plate and frame filter pressing on the obtained flocculation liquid of the N-acetylglucosamine fermentation liquid to obtain the N-acetylglucosamine fermentation clear liquid.
Further, in the step (1.1), the inactivation of the N-acetylglucosamine fermentation broth is carried out by heating at 80-100 deg.C for 20-40 min.
Further, in the step (1.2), the inorganic flocculant is one of polymeric ferric sulfate, polymeric aluminum chloride and polymeric aluminum ferric silicate, and the temperature of the acetylglucosamine fermentation liquor is 80-100 ℃ when the inorganic flocculant is added.
Further, in the step (1.2), the dosage of the inorganic flocculant is based on the pH, and the pH of the inactivated N-acetylglucosamine fermentation liquor after the inorganic flocculant is added is 4.5-5.5.
Further, in the step (1.3), the filter aid is one of diatomite and perlite; the addition amount of the filter aid is 5-15 per mill of the volume of the N-acetylglucosamine fermentation liquor;
the adsorbent is activated carbon, and the addition of the adsorbent is 1-10 per mill (W/V) of the volume of the N-acetylglucosamine fermentation liquid.
Further, in the step (1.3), the organic flocculant is a natural organic polymeric flocculant-chitin solution, and the concentration of chitin in the chitin solution is 1-4%; the dosage of the organic polymeric flocculant chitin solution is 0.1-1.8 per mill (V/V) of the volume of the N-acetylglucosamine fermentation liquor.
Further, in the step (1.3), the flocculation conditions are: adding inorganic flocculant at 80-100 deg.C, adding inorganic flocculant to obtain N-acetylglucosamine fermentation liquid with pH of 4.5-5.5 and organic flocculant-chitin solution amount of 0.1-1.0 ‰, stirring, and standing for 30-60 min.
Further, in the step (1.4), the operation method of the plate-and-frame filter pressing is as follows: coating a plate-frame filter cloth with diatomite, pumping N-acetylglucosamine fermentation flocculation liquid into a plate frame by using the pressure of 6kgf to maintain the internal pressure of the plate frame at 1-4kgf, and performing top washing on a bacterium cake by using pure water with the volume of 10-30% of the volume of the N-acetylglucosamine fermentation liquid after the flocculation hydraulic filtration is finished.
The method takes the fermentation broth of the acetamido sugar produced by fermentation as a raw material, and adds the inorganic flocculant to acidify the fermentation broth and provide a large amount of inorganic complex ions at the same time, so as to strongly adsorb colloid particles of thalli and destroy the stability of thalli and metabolite colloids, thereby achieving primary flocculation; then the self-bridging and adsorption effects of the organic polymer flocculant are used, and thalli and metabolic impurity particles in the fermentation solution are flocculated under the net catching effect of active groups on the organic polymer. Because the salt in the inorganic flocculant exists, the surface charge of the colloid is better neutralized, the flocculation effect of the organic polymer is promoted, and the flocculation effect is greatly improved.
The inorganic and organic composite flocculant is used in combination, which not only makes the plate-frame filtration smoothly realized, but also greatly reduces the dosage of organic flocculant-chitin and reduces the cost of flocculation process.
The method has the advantages that the quality of the fermentation clear liquid of the acetylglucosamine is high, the operation flow is simple, the floc is easy to separate, the cost is low and the like, and the yield of the N-acetylglucosamine is high; and the natural organic polymer chitin is used as the organic flocculant, so that the 'three-induced' effect (causing Kazaki, carcinogenesis and mutation) of residual monomers of high polymers is avoided, the whole process is clean and environment-friendly, and the aims of no toxicity and high efficiency are fulfilled.
Has the advantages that: compared with the prior art, the method has the advantages that a large amount of complex ions provided by the inorganic polymer flocculant strongly adsorb thallus colloid particles, and then the active groups adsorbed on the organic polymer are meshed to capture other impurity particles to sink together through the self-bridging action of the organic polymer flocculant; the existence of inorganic salt can also neutralize the surface charges of thalli and impurity particles, and can promote the flocculation of organic macromolecules, thereby greatly improving the flocculation effect; firstly, utilizing large intestine engineering bacteria to carry out high-density fermentation to produce the acetylglucosamine, adding a proper amount of inorganic flocculant into fermentation liquor to adjust the pH value to 4.5-5.5, then adding 0.1-1.8 per mill of natural organic polymer flocculant chitin, stirring uniformly, standing and flocculating for 30-60min, finally adding a proper amount of active carbon and diatomite filter aid, and then carrying out plate-frame filtration to remove high-concentration thalli and other metabolic impurities in the fermentation liquor, thus obtaining pure N-acetylglucosamine clear liquid. The flocculation impurity removal process has the advantages of obvious effect, high yield of clear liquid after plate-frame filtration, low water content of mushroom dregs and reduction of the dosage of a flocculating agent by composite flocculation. The method is simple to operate, does not discharge three wastes, and is an environment-friendly, simple and efficient method for preparing the acetamido sugar fermentation clear liquid.
Drawings
FIG. 1 is a flow chart of the architecture of the present invention.
Detailed Description
The invention relates to a method for preparing N-acetylglucosamine fermentation clear liquid by using a composite flocculant, which comprises the following specific operation steps:
(1.1) inactivating the N-acetylglucosamine fermentation liquor produced by high-density fermentation of large intestine engineering bacteria;
(1.2) adding an inorganic flocculant into the inactivated N-acetylglucosamine fermentation liquor, and uniformly stirring to obtain the N-acetylglucosamine fermentation liquor containing the inorganic flocculant;
(1.3) adding an adsorbent and a filter aid into the N-acetylglucosamine fermentation liquor containing the inorganic flocculant, uniformly stirring, adding a prepared organic polymeric flocculant, namely 1-4% chitin solution, uniformly stirring, standing for flocculation for 30-60min, and thus obtaining a flocculation liquid of the N-acetylglucosamine fermentation liquor;
(1.4) finally, carrying out plate and frame filter pressing on the obtained flocculation liquid of the N-acetylglucosamine fermentation liquid to obtain the N-acetylglucosamine fermentation clear liquid.
Further, in the step (1.1), the inactivation of the N-acetylglucosamine fermentation broth is carried out by heating at 80-100 deg.C for 20-40 min.
Further, in the step (1.2), the inorganic flocculant is one of polymeric ferric sulfate, polymeric aluminum chloride and polymeric aluminum ferric silicate, and the temperature of the N-acetylglucosamine fermentation liquor is 80-100 ℃ when the inorganic flocculant is added.
Further, in the step (1.2), the dosage of the inorganic flocculant is based on the pH, and the pH of the inactivated N-acetylglucosamine fermentation liquor after the inorganic flocculant is added is 4.5-5.5.
Further, in the step (1.3), the filter aid is one of diatomite and perlite; the addition amount of the filter aid is 5-15 per mill of the volume of the N-acetylglucosamine fermentation liquor;
the adsorbent is activated carbon, and the addition of the adsorbent is 1-10 per mill (W/V) of the volume of the N-acetylglucosamine fermentation liquid.
Further, in the step (1.3), the organic flocculant is a natural organic polymeric flocculant-chitin solution, and the concentration of chitin in the chitin solution is 1-4%; the organic polymer flocculant chitin solution is 0.1-1.8 per mill (V/V) of the volume of the N-acetylglucosamine fermentation liquor.
Further, in the step (1.3), the flocculation conditions are: adding inorganic flocculant at 80-100 deg.C, adding inorganic flocculant to obtain N-acetylglucosamine fermentation liquid with pH of 4.5-5.5, adding organic flocculant, chitin solution in an amount of 0.1-1.8 ‰, stirring, stopping stirring, and standing for 30-60 min.
Further, in the step (1.4), the operation method of the plate-and-frame filter pressing is as follows: coating a plate-frame filter cloth with diatomite, pumping N-acetylglucosamine fermentation flocculation liquid into a plate frame by using the pressure of 6kgf to maintain the internal pressure of the plate frame at 1-4kgf, and performing top washing on a bacterium cake by using pure water with the volume of 10-30% of the volume of the N-acetylglucosamine fermentation liquid after the flocculation hydraulic filtration is finished.
Example 1:
taking 40L of N-acetylglucosamine fermentation broth with high density of engineering bacteria, wherein the fermentation broth OD is 97, and heating to 85 ℃ by a jacket for inactivation; stirring to 450rpm, adding 200g (W/V) of polymeric ferric sulfate, adjusting the pH value to 4.5, adding 160g of active carbon and 240g of diatomite while stirring, stirring for 10min, reducing the stirring speed to 110rpm, then adding 100ml of 2% chitin solution, uniformly stirring, reducing the stirring speed to 50rpm, simultaneously reducing the temperature to 50 ℃, stopping stirring, and standing for flocculation for 30 min; then plate-frame filtration is carried out; after the fermentation liquid completely passes through, carrying out top water treatment by using 30% pure water, and drying the plate frame by blowing, thereby finally obtaining 45L of N-acetylglucosamine fermentation clear liquid, wherein the yield of the acetylglucosamine reaches 99%, the bacterial sludge filter cake is 7kg, and the water content is 68%.
Example 2:
taking 340L of N-acetylglucosamine density fermentation liquor with high engineering bacteria, wherein the bacterial concentration OD of the fermentation liquor is 110, and heating to 95 ℃ by a jacket for inactivation; stirring to 300rpm after inactivation, adding 2.0kg of polyferric sulfate to adjust the pH value to 5.0, simultaneously adding 4.0kg of active carbon and 6.0kg of diatomite while stirring, stirring for 10min, reducing the stirring speed to 110rpm, adding 600ml of chitin solution with the concentration of 3%, stirring for 10min, cooling to 50 ℃, continuing to stir at the speed of 50rpm for 60min, flocculating, performing plate-and-frame filtration, filtering for 3h, after the fermentation solution is completely filtered, washing a filter cake by using 20% pure water, and drying the plate-and-frame to obtain 370L of the acetylglucosamine fermentation clear liquid, wherein the yield of the N-acetylglucosamine is 98%; 79kg of bacterial sludge filter cake, and the water content is 74 percent.
Example 3:
taking 4t of N-acetylglucosamine fermentation broth with high engineering bacteria density, wherein the fermentation broth OD is 82, and heating to 95 ℃ by a jacket for inactivation; after inactivation, beating the fermentation liquor into a flocculation tank of 10t, stirring to 50Hz, adding 20kg of polyferric sulfate to adjust the pH to 4.5, adding 16kg of active carbon and 24kg of diatomite while stirring, stirring for 10min, reducing the stirring speed to 30Hz, adding 10L of 2% chitin solution, stirring for 10min, cooling to 50 ℃, stopping stirring and flocculating for 60min, and then performing plate-and-frame filtration; after the fermentation liquid is completely filtered, washing a filter cake by using 10% pure water, and drying a plate frame by blowing, thereby finally obtaining 3.7t of N-acetylglucosamine fermentation clear liquid and the yield of the N-acetylglucosamine is 98%; 650kg of bacterial sludge filter cake with water content of 68 percent.
Claims (8)
1. A method for preparing N-acetylglucosamine fermentation clear liquid by using a composite flocculant is characterized by comprising the following specific operation steps:
(1.1) inactivating the N-acetylglucosamine fermentation liquor produced by high-density fermentation of large intestine engineering bacteria;
(1.2) adding an inorganic flocculant into the inactivated N-acetylglucosamine fermentation liquor, and uniformly stirring to obtain the N-acetylglucosamine fermentation liquor containing the inorganic flocculant;
(1.3) adding an adsorbent and a filter aid into the N-acetylglucosamine fermentation liquor containing the inorganic flocculant, uniformly stirring, adding the prepared organic polymeric flocculant, uniformly stirring, standing for flocculation for 30-60min, and thus obtaining a flocculation liquid of the N-acetylglucosamine fermentation liquor;
(1.4) finally, carrying out plate and frame filter pressing on the obtained flocculation liquid of the N-acetylglucosamine fermentation liquid to obtain the N-acetylglucosamine fermentation clear liquid.
2. The method for preparing N-acetylglucosamine fermentation supernatant by using composite flocculant according to claim 1, wherein in step (1.1), the inactivation of N-acetylglucosamine fermentation broth is carried out by heating at 80-100 deg.C for 20-40 min.
3. The method for preparing clear fermented solution of N-acetylglucosamine with compound flocculant according to claim 1, wherein in step (1.2), the inorganic flocculant is one of polymeric ferric sulfate, polymeric aluminum chloride and polymeric aluminum ferric silicate, and the temperature of the fermented solution of N-acetylglucosamine is 80-100 ℃ when the inorganic flocculant is added.
4. The method for preparing clear fermentation liquid of N-acetylglucosamine by using composite flocculant of claim 1, wherein in step (1.2), the inorganic flocculant is used in an amount based on the pH, and the pH of the inactivated fermentation liquid of N-acetylglucosamine is 4.5-5.5 after the inorganic flocculant is added.
5. The method for preparing N-acetylglucosamine fermentation supernatant by using composite flocculant according to claim 1, wherein in step (1.3), the filter aid is one of diatomaceous earth and perlite; the addition amount of the filter aid is 5-15 per mill of the volume of the N-acetylglucosamine fermentation liquor;
the adsorbent is activated carbon, and the addition of the adsorbent is 1-10 per mill of the volume of the N-acetylglucosamine fermentation liquid.
6. The method for preparing clear fermented N-acetylglucosamine solution by using composite flocculant according to claim 1, wherein in step (1.3), the organic flocculant is chitin solution, which is a natural organic polymeric flocculant, and the concentration of chitin in the chitin solution is 1-4%; the dosage of the organic polymeric flocculant chitin solution is 0.1-1.8 per mill of the volume of the N-acetylglucosamine fermentation liquor.
7. The method for preparing N-acetylglucosamine fermentation supernatant by using composite flocculant of claim 1, wherein in step (1.3), the flocculation condition is: adding inorganic flocculant at 80-100 deg.C, adding inorganic flocculant to obtain N-acetylglucosamine fermentation liquid with pH of 4.5-5.5 and organic flocculant-chitin solution amount of 0.1-1.8 ‰, stirring, and standing for 30-60 min.
8. The method for preparing N-acetylglucosamine fermentation liquor by using a composite flocculant according to claim 1, wherein in the step (1.4), the operation method of plate-and-frame filter pressing is as follows: coating a plate frame filter cloth with diatomite, pumping the N-acetylglucosamine fermentation flocculation liquid into a plate frame by using the pressure of 6kgf to maintain the internal pressure of the plate frame at 1-4kgf, and carrying out top washing on the bacterial cake by using pure water with the volume of 10-30% of the N-acetylglucosamine fermentation flocculation liquid after the flocculation hydraulic filtration is finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110630095.5A CN113354698A (en) | 2021-06-07 | 2021-06-07 | Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110630095.5A CN113354698A (en) | 2021-06-07 | 2021-06-07 | Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113354698A true CN113354698A (en) | 2021-09-07 |
Family
ID=77532693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110630095.5A Pending CN113354698A (en) | 2021-06-07 | 2021-06-07 | Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113354698A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114957349A (en) * | 2022-06-30 | 2022-08-30 | 宁夏华吉生物有限公司 | Pretreatment and filtration method of acarbose fermentation liquor |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102603132A (en) * | 2012-02-29 | 2012-07-25 | 波鹰(厦门)科技有限公司 | Sludge treatment device based on electrolysis and pressure filtration technologies and method thereof |
| CN104402976A (en) * | 2014-12-29 | 2015-03-11 | 江西兴鼎科技有限公司 | Method for preparing enramycin refined powder |
| CN104788510A (en) * | 2015-04-29 | 2015-07-22 | 江苏赛奥生化有限公司 | Method for extracting glucosamine from self-fermentation liquid |
| CN105543312A (en) * | 2016-01-29 | 2016-05-04 | 浙江澳兴生物科技有限公司 | Preparation method of microbial fermentation-based N-acetyle-D-glucosamine |
| CN107354188A (en) * | 2017-08-12 | 2017-11-17 | 河南巨龙生物工程股份有限公司 | The technique of ETEC JL GlcN fermenting and producing N acetylglucosamines |
| CN107653274A (en) * | 2017-11-03 | 2018-02-02 | 山东润德生物科技有限公司 | Glucosamine feeding medium during fermentation control method based near infrared spectrum quick test |
| CN111647027A (en) * | 2020-06-11 | 2020-09-11 | 江苏海飞生物科技有限公司 | Method for separating and purifying N-acetylglucosamine |
| CN111792750A (en) * | 2020-06-17 | 2020-10-20 | 中国科学院青岛生物能源与过程研究所 | Fermentation liquor pretreatment method by coupling flocculation adsorption |
| CN113373134A (en) * | 2021-07-30 | 2021-09-10 | 江苏海飞生物科技有限公司 | Extraction method of N-acetylglucosamine deacetylase |
| WO2023280257A1 (en) * | 2021-07-08 | 2023-01-12 | 杭州中美华东制药江东有限公司 | Pretreatment process for acarbose fermentation liquid |
-
2021
- 2021-06-07 CN CN202110630095.5A patent/CN113354698A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102603132A (en) * | 2012-02-29 | 2012-07-25 | 波鹰(厦门)科技有限公司 | Sludge treatment device based on electrolysis and pressure filtration technologies and method thereof |
| CN104402976A (en) * | 2014-12-29 | 2015-03-11 | 江西兴鼎科技有限公司 | Method for preparing enramycin refined powder |
| CN104788510A (en) * | 2015-04-29 | 2015-07-22 | 江苏赛奥生化有限公司 | Method for extracting glucosamine from self-fermentation liquid |
| CN105543312A (en) * | 2016-01-29 | 2016-05-04 | 浙江澳兴生物科技有限公司 | Preparation method of microbial fermentation-based N-acetyle-D-glucosamine |
| CN107354188A (en) * | 2017-08-12 | 2017-11-17 | 河南巨龙生物工程股份有限公司 | The technique of ETEC JL GlcN fermenting and producing N acetylglucosamines |
| CN107653274A (en) * | 2017-11-03 | 2018-02-02 | 山东润德生物科技有限公司 | Glucosamine feeding medium during fermentation control method based near infrared spectrum quick test |
| CN111647027A (en) * | 2020-06-11 | 2020-09-11 | 江苏海飞生物科技有限公司 | Method for separating and purifying N-acetylglucosamine |
| US20220324896A1 (en) * | 2020-06-11 | 2022-10-13 | Jiangsu Harvers Biotech Co., Ltd. | Method for Separation and Purification of N-acetylglucosamine |
| CN111792750A (en) * | 2020-06-17 | 2020-10-20 | 中国科学院青岛生物能源与过程研究所 | Fermentation liquor pretreatment method by coupling flocculation adsorption |
| WO2023280257A1 (en) * | 2021-07-08 | 2023-01-12 | 杭州中美华东制药江东有限公司 | Pretreatment process for acarbose fermentation liquid |
| CN113373134A (en) * | 2021-07-30 | 2021-09-10 | 江苏海飞生物科技有限公司 | Extraction method of N-acetylglucosamine deacetylase |
Non-Patent Citations (1)
| Title |
|---|
| 王乾乾，等: "甲壳素及其衍生物在皮革工业中的应用", 中国皮革, vol. 40, no. 17, pages 25 - 29 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114957349A (en) * | 2022-06-30 | 2022-08-30 | 宁夏华吉生物有限公司 | Pretreatment and filtration method of acarbose fermentation liquor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11555049B2 (en) | Method for separation and purification of n-acetylglucosamine | |
| CN110590867B (en) | Synthesis method of D-glucosamine hydrochloride | |
| CN106957129B (en) | Method for treating riboflavin fermentation liquor | |
| CN111018926B (en) | Method for extracting high-purity glucosamine hydrochloride from glucosamine fermentation broth | |
| CN101704905B (en) | Method for extracting hyaluronic acid | |
| CN106831895A (en) | A kind of method of purifying N acetylglucosamines | |
| CN104788510A (en) | Method for extracting glucosamine from self-fermentation liquid | |
| CN107177648A (en) | A kind of chitosan oligosaccharide enzymatic production process | |
| CN113354698A (en) | Method for preparing N-acetylglucosamine fermentation clear liquid by using composite flocculant | |
| CN115746067A (en) | Solid-liquid separation method of fermentation liquor | |
| CN108285913B (en) | Process for preparing and extracting L-glutamine | |
| CN104059166A (en) | Method for preparing oligomeric hyaluronic acid by hyaluronic acid broth | |
| CN103667382B (en) | A kind of fermentable produces the method for L-glutaminate | |
| CN104059169B (en) | A kind of hyaluronic purifying technique | |
| KR20200059089A (en) | The Flocculation Agent For Yeast and The Mothod of Producing It | |
| CN102092892A (en) | Treatment method of production wastewater of vitamin C and derivatives of vitamin C | |
| CN103319628A (en) | Method for preparing chondroitin sulfate by virtue of ultrahigh-pressure micro-jet ultrafiltration | |
| CN112608959B (en) | Method for improving fermentation unit of acetylglucosamine | |
| CN104694614A (en) | Novel extraction process of L-tryptophan | |
| CN103667383B (en) | The preparation method of L-glutaminate | |
| CN113774104A (en) | Method for preparing peptone and bio-organic fertilizer by using chondroitin sulfate wastewater | |
| CN113248553A (en) | Preparation method of D-glucosamine hydrochloride | |
| CN110857445A (en) | High-purity low-energy-consumption lactic acid production process | |
| CN103113334B (en) | Method for separating mycelium in vitamin C fermentation broth by environment-friendly montmorillonite flocculant | |
| CN111893152B (en) | Method for biosynthesizing chitosan by using bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |