CN113349302A - Efficient utilization type fermented feed special for piglets and capable of improving immune function - Google Patents
Efficient utilization type fermented feed special for piglets and capable of improving immune function Download PDFInfo
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- CN113349302A CN113349302A CN202110666317.9A CN202110666317A CN113349302A CN 113349302 A CN113349302 A CN 113349302A CN 202110666317 A CN202110666317 A CN 202110666317A CN 113349302 A CN113349302 A CN 113349302A
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- parts
- piglets
- lactic acid
- acid bacteria
- culture medium
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- 230000036737 immune function Effects 0.000 title claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 64
- 230000004151 fermentation Effects 0.000 claims abstract description 64
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 52
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- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims abstract description 11
- 235000013734 beta-carotene Nutrition 0.000 claims abstract description 11
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- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 10
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- -1 compound vitamin Chemical class 0.000 claims description 11
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 10
- 108091005508 Acid proteases Proteins 0.000 claims description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 235000019764 Soybean Meal Nutrition 0.000 claims description 9
- 239000004455 soybean meal Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 7
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 6
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 6
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 6
- 235000019155 vitamin A Nutrition 0.000 claims description 6
- 239000011719 vitamin A Substances 0.000 claims description 6
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 5
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 5
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- 229930003316 Vitamin D Natural products 0.000 claims description 5
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 5
- 229930003427 Vitamin E Natural products 0.000 claims description 5
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 5
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 5
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 5
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 5
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 5
- 229960003512 nicotinic acid Drugs 0.000 claims description 5
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 5
- ILRLTAZWFOQHRT-UHFFFAOYSA-N potassium;sulfuric acid Chemical compound [K].OS(O)(=O)=O ILRLTAZWFOQHRT-UHFFFAOYSA-N 0.000 claims description 5
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- 239000011780 sodium chloride Substances 0.000 claims description 5
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- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 5
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- 229940046009 vitamin E Drugs 0.000 claims description 5
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- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims 1
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- 108091005804 Peptidases Proteins 0.000 abstract description 2
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- 240000008620 Fagopyrum esculentum Species 0.000 abstract 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 1
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a piglet special high-efficiency utilization type fermented feed for improving immune function, which relates to the technical field of piglet feeds and comprises the following components: the feed is characterized by comprising lactobacillus fermentation liquor, bacillus subtilis culture solution, lactobacillus sojae atricolor, lactobacillus fermentum TLa-6, acidic protease, neutral protease, lysine, tryptophan, valine, threonine, yeast powder, skim milk powder, lactose, corn flour, beef extract, bean dregs, figs, buckwheat flour, beta-carotene, compound inorganic minerals and compound vitamins.
Description
Technical Field
The invention relates to the technical field of piglet feed, in particular to a piglet special high-efficiency utilization type fermented feed for improving immune function.
Background
The term "feed" is a general term for food of all human-raised animals, and in a narrow sense, a general feed mainly refers to food of agricultural or animal-raised animals. The Feed (Feed) comprises more than ten kinds of Feed raw materials such as soybean, soybean meal, corn, fish meal, amino acid, miscellaneous meal, whey powder, grease, meat and bone meal, grains, Feed additives and the like.
The feed industry in China is a new industry, the total yield of commercial feed in 2016 years in China reaches 20918 ten thousand t, and the total yield is 8014 hundred million yuan. Classified according to the level of meeting the nutritional needs of animals, feed products can be divided into:
(1) complete compound feed. Contains feed meeting the requirements of animals on protein, energy, macrominerals, trace minerals, vitamins and various nutritional and non-nutritional additives. Can be directly fed to animals.
(2) And (4) concentrating the feed. Usually, the energy feed in the feed formula, such as corn, wheat, bran, etc., accounts for more than 50% of the formula, and the raw materials are most purchased by breeding manufacturers or other manufacturers, in order to reduce the transportation cost, the feed manufacturers design feed products without energy raw materials, and the breeding manufacturers can produce complete compound feed with relatively low cost after purchasing the products and mixing the products with the self-produced or self-purchased energy raw materials.
(3) And (4) premixing feed. In order to further meet the requirements of scale feeders on refined feeding and cost reduction, feed producers design premixed feed products, which mainly contain minerals, vitamins and various nutritional and non-nutritional additives. The product has the characteristics of low addition proportion, high nutrient concentration, high mixing difficulty, high product value, high technical content and high storage requirement.
The pig is taken as the main edible meat for people, rich nutrient substances are provided for people, the feed for the piglets is not as coarse as the feed for adult pigs, the feed for the piglets needs to be healthy and nutritious, the taste is liked by the piglets, the nutrient substances added into the feed for the piglets on the market are insufficient, the later development of the piglets is influenced, the vitamins and intestinal flora in the piglets are few, the growth retardation of the piglets is easily caused, and the resistance is low.
Disclosure of Invention
In order to solve the technical problems, the invention provides a piglet special high-efficiency utilization type fermented feed for improving the immunity.
The technical scheme of the invention is as follows: a piglet special high-efficiency utilization type fermented feed for improving immune function comprises the following components in parts by weight: 10-20 parts of lactobacillus fermentation liquor, 3-5 parts of bacillus subtilis culture solution, 1.2-2.2 parts of soybean meal lactobacillus, 1.3-1.5 parts of lactobacillus fermentum TLa-6, 0.1-1 part of acid protease, 0.1-1 part of neutral protease, 5-8 parts of lysine, 2-10 parts of tryptophan, 2-8 parts of valine, 5-9 parts of threonine, 0.5-2 parts of yeast powder, 5-10 parts of skim milk powder, 7-9 parts of lactose, 30-50 parts of corn, 3-8 parts of beef, 10-20 parts of bean dregs, 8-13 parts of fig, 10-15 parts of buckwheat, 0.3-1 part of beta-carotene, 5-9 parts of compound inorganic mineral substances and 1-5 parts of compound vitamins.
Further, the preparation method of the fermented feed comprises the following steps:
s1: grinding:
respectively grinding 30-50 parts of corn and 10-15 parts of buckwheat in a grinder to obtain corn flour and buckwheat flour;
s2: mincing meat:
putting 3-8 parts of beef into a meat grinder, starting the meat grinder to grind the beef, wherein the rotation speed of the meat grinder is 80-120min, the meat grinding time is 10-15min, and after the meat grinding is finished, beef extract is obtained;
s3: primary mixing:
putting the corn flour, the buckwheat flour and the beef extract obtained in the steps S1 and S2 into a mixer, taking 10-20 parts of bean dregs, 8-13 parts of fig, 1.2-2.2 parts of soybean meal lactobacillus, 1.3-1.5 parts of lactobacillus fermentum TLa-6, 0.1-1 part of acid protease, 0.1-1 part of neutral protease, 5-8 parts of lysine, 2-10 parts of tryptophan, 2-8 parts of valine, 5-9 parts of threonine, 0.5-2 parts of yeast powder, 5-10 parts of skimmed milk powder, 7-9 parts of lactose, 0.3-1 part of beta-carotene, 5-9 parts of inorganic mineral substances and 1-5 parts of compound vitamin, mixing at the mixer temperature of 20-30 ℃, the mixer rotating speed of 60-80r/min for 10-20min, obtaining a primary mixed material;
s4 final mixing:
pouring 10-20 parts of lactobacillus fermentation liquor and 3-5 parts of bacillus subtilis culture solution into a mixer to be mixed with the primary mixed material in the step S3, and stirring at the speed of 70-100r/min for 10-15min to obtain a fermentation material;
s5: fermentation:
and pouring the fermented material into a sealed fermentation tank for fermentation for 50-80h at 25-35 ℃ to obtain the fermented feed.
Further, the composite inorganic mineral comprises the following components in parts by weight: 0.4-1.2 parts of ferrous sulfate heptahydrate, 0.6-0.9 part of copper sulfate pentahydrate, 0.3-0.5 part of zinc sulfate heptahydrate, 0.8-1.4 parts of manganese sulfate monohydrate, 1.1-1.4 parts of magnesium sulfate, 0.8-1.3 parts of sodium chloride, 0.5-1.4 parts of potassium dihydrogen sulfate and 0.5-0.9 part of calcium dihydrogen phosphate.
Further, the compound vitamin comprises the following components in parts by weight: 0.1-0.5 part of vitamin A, 0.2-0.9 part of vitamin C, 0.2-0.8 part of vitamin D, 0.2-1.1 part of vitamin E, 0.1-0.6 part of vitamin B1, 0.1-0.4 part of vitamin B2 and 0.1-0.7 part of vitamin B3, and can supplement vitamins in piglets and enhance piglet immunity.
Further, the preparation method of the lactic acid bacteria culture solution comprises the following steps: firstly, sterilizing a lactic acid bacteria culture medium at the temperature of 100-120 ℃ for 10-15min, then naturally cooling to room temperature, inoculating a lactic acid bacteria strain on the lactic acid bacteria culture medium for culturing for 1 day, then sterilizing a lactic acid bacteria fermentation culture medium in the same way as the lactic acid bacteria culture medium, then inoculating the lactic acid bacteria culture medium into the lactic acid bacteria fermentation culture medium, fermenting at the temperature of 20-30 ℃ for 8-15h, and culturing the lactic acid bacteria after fermentation is completed, wherein the fermented feed has good taste.
Further, the pH value of the lactic acid bacteria culture medium and the lactic acid bacteria fermentation culture medium is 6.5-7.5.
Further, the preparation method of the culture solution of the bacillus subtilis comprises the following steps: preparing a bacillus subtilis culture medium from the bone soup, inoculating bacillus subtilis strains in the bacillus subtilis culture medium, and culturing in an aerobic environment for 40-80h to prepare a bacillus subtilis culture solution, so that the digestion gastrointestinal flora of piglets is enhanced, and the digestion capacity of the piglets is further promoted.
Further, the bone soup is prepared from animal bones and water according to the mass ratio of 1:6, and then is decocted for 12-24h with slow fire, which is beneficial to culturing the bacillus subtilis.
Further, the culture temperature of the bacillus subtilis is 20-30 ℃, and the suitable temperature enhances the reproductive capacity of the bacillus subtilis.
Further, in the step S1, the rotation speed of the grinder is 80-120r/min, the grinding time is 30-60min, so that the corn and the buckwheat are quickly powdered, the granularity of the corn flour and the buckwheat is 20-55 mu m, the granularity is small, the fermentation time of the feed is shortened, and the contact area of the feed with the corn flour and the buckwheat is increased.
The invention has the beneficial effects that:
(1) the invention supplements organic matters and vitamins in piglets, and is beneficial to the development and the immunity enhancement of the piglets.
(2) The fermented feed is rich in amino acid and beneficial to synthesis of in-vivo protein, the piglet feed contains bacillus and protease to promote decomposition of in-vivo protein of piglets and intestinal beneficial bacteria and enhance digestion capacity of piglets, and the fermented feed contains fig which contains fructose to enhance mouthfeel, is rich in nutrition and is beneficial to piglet development.
Drawings
FIG. 1 is a schematic view of the preparation process of the fermented feed of the present invention.
Detailed Description
Example 1:
a piglet special high-efficiency utilization type fermented feed for improving immune function comprises the following components in parts by weight: 10 parts of lactobacillus fermentation liquor, 3 parts of bacillus subtilis culture solution, 1.2 parts of lactobacillus sojae atricolor, 1.3 parts of lactobacillus fermentum TLa-6, 0.1 part of acid protease, 0.1 part of neutral protease, 5 parts of lysine, 2 parts of tryptophan, 2 parts of valine, 5 parts of threonine, 0.5 part of yeast powder, 5 parts of skimmed milk powder, 7 parts of lactose, 30 parts of corn, 3 parts of beef, 10 parts of bean dregs, 8 parts of fig, 10 parts of buckwheat, 0.3 part of beta-carotene, 5 parts of compound inorganic mineral substances and 1 part of compound vitamin;
the composite inorganic mineral comprises the following components in parts by weight: 0.4 part of ferrous sulfate heptahydrate, 0.6 part of copper sulfate pentahydrate, 0.3 part of zinc sulfate heptahydrate, 0.8 part of manganese sulfate monohydrate, 1.1 part of magnesium sulfate, 0.8 part of sodium chloride, 0.5 part of potassium dihydrogen sulfate and 0.5 part of calcium dihydrogen phosphate, and the inorganic mineral substances in piglets are supplemented, and the piglet development is promoted;
the compound vitamin comprises the following components in parts by weight: 0.1 part of vitamin A, 0.2 part of vitamin C, 3 parts of vitamin D, 0.2 part of vitamin E, 0.1 part of vitamin B1, 0.1 part of vitamin B2 and 0.1 part of vitamin B3, and the vitamin A can supplement vitamins in piglets and enhance the immunity of the piglets;
the preparation method of the lactic acid bacteria culture solution comprises the following steps: firstly, sterilizing a lactic acid bacteria culture medium at 100 ℃ for 10min, then naturally cooling to room temperature, inoculating a lactic acid bacteria strain on the lactic acid bacteria culture medium, culturing for 1 day, then sterilizing a lactic acid bacteria fermentation culture medium, wherein the sterilization mode is the same as that of the lactic acid bacteria culture medium, then inoculating the lactic acid bacteria culture medium into the lactic acid bacteria fermentation culture medium, the fermentation temperature is 20 ℃, fermenting for 8 hours, the pH values of the lactic acid bacteria culture medium and the lactic acid bacteria fermentation culture medium are 6.5, and the obtained lactic acid bacteria culture medium after fermentation is completed has good mouthfeel of fermented feed;
the preparation method of the culture solution of the bacillus subtilis comprises the following steps: preparing a bacillus subtilis culture medium from bone soup, wherein the bone soup is prepared from animal bones and water according to the mass ratio of 1:6, then decocting the bone soup with slow fire for 12-24 hours, so that the bacillus subtilis is cultured, inoculating bacillus subtilis strains in the bacillus subtilis culture medium, culturing the bacillus subtilis in an aerobic environment for 40-80 hours, and culturing the bacillus subtilis at the temperature of 20-30 ℃ at a proper temperature to enhance the reproductive capacity of the bacillus subtilis; preparing a culture solution of bacillus subtilis to enhance the digestion gastrointestinal flora of piglets and further promote the digestion capacity of piglets;
as shown in figure 1, the preparation method of the fermented feed comprises the following steps:
s1: grinding:
respectively putting 30 parts of corn and 10 parts of buckwheat into a grinder for grinding, wherein the rotation speed of the grinder is 80r/min, the grinding time is 30min, so that the corn and the buckwheat are quickly powdered, the granularity of the corn flour and the buckwheat is 20 mu m, the granularity is small, the fermentation time of the feed is shortened, the contact area of the feed and the corn flour and the buckwheat is increased, and the corn flour and the buckwheat flour are obtained;
s2: mincing meat:
putting 3 parts of beef into a meat grinder, starting the meat grinder to grind the beef, wherein the rotation speed of the meat grinder is 80min, the meat grinding time is 10min, and after the meat grinding is finished, beef extract is obtained;
s3: primary mixing:
putting the corn flour, the buckwheat flour and the beef extract obtained in the steps S1 and S2 into a mixer, and then putting 10 parts of bean dregs, 8 parts of fig, 1.2 parts of soybean meal lactobacillus, 1.3 parts of lactobacillus fermentum TLa-6, 0.1 part of acid protease, 0.1 part of neutral protease, 5 parts of lysine, 2 parts of tryptophan, 2 parts of valine, 5 parts of threonine, 0.5 part of yeast powder, 5 parts of skimmed milk powder, 7 parts of lactose, 0.3 part of beta-carotene, 5 parts of inorganic mineral substances and 1 part of compound vitamin into the mixer for mixing, wherein the temperature of the mixer is 20 ℃, the rotating speed of the mixer is 60r/min, and the mixing time is 10min to obtain a primary mixed material;
s4 final mixing:
pouring 10 parts of lactobacillus fermentation liquor and 3 parts of bacillus subtilis culture solution into a mixer to be mixed and stirred with the primary mixed material in the step S3, wherein the stirring speed is 70r/min, and the stirring time is 10min, so as to obtain a fermented material;
s5: fermentation:
and pouring the fermented material into a sealed fermentation tank for fermentation, wherein the fermentation time is 50h, the fermentation temperature is 25 ℃, and the fermented feed is obtained after fermentation.
Example 2:
a piglet special high-efficiency utilization type fermented feed for improving immune function comprises the following components in parts by weight: 15 parts of lactobacillus fermentation liquor, 4 parts of bacillus subtilis culture solution, 1.8 parts of lactobacillus sojae atricolor, 1.4 parts of lactobacillus fermentum TLa-6, 0.5 part of acid protease, 0.5 part of neutral protease, 6 parts of lysine, 7 parts of tryptophan, 5 parts of valine, 7 parts of threonine, 1.3 parts of yeast powder, 8 parts of skimmed milk powder, 8 parts of lactose, 40 parts of corn, 6 parts of beef, 15 parts of bean dregs, 10 parts of fig, 13 parts of buckwheat, 0.8 part of beta-carotene, 8 parts of compound inorganic mineral substances and 3 parts of compound vitamin;
the composite inorganic mineral comprises the following components in parts by weight: 0.9 part of ferrous sulfate heptahydrate, 0.7 part of copper sulfate pentahydrate, 0.4 part of zinc sulfate heptahydrate, 1.0 part of manganese sulfate monohydrate, 1.2 parts of magnesium sulfate, 1.1 part of sodium chloride, 1.1 part of potassium dihydrogen sulfate and 0.8 part of calcium dihydrogen phosphate, and the inorganic mineral substances in piglets are supplemented, and the piglet development is promoted;
the compound vitamin comprises the following components in parts by weight: 0.4 part of vitamin A, 0.6 part of vitamin C, 0.5 part of vitamin D, 0.8 part of vitamin E, 0.4 part of vitamin B1, 0.2 part of vitamin B2 and 0.6 part of vitamin B3, and can supplement vitamins in piglets and enhance the immunity of piglets;
the preparation method of the lactic acid bacteria culture solution comprises the following steps: firstly, sterilizing a lactic acid bacteria culture medium at 110 ℃ for 13min, then naturally cooling to room temperature, inoculating a lactic acid bacteria strain on the lactic acid bacteria culture medium, culturing for 1 day, then sterilizing a lactic acid bacteria fermentation culture medium, wherein the sterilization mode is the same as that of the lactic acid bacteria culture medium, then inoculating the lactic acid bacteria culture medium into the lactic acid bacteria fermentation culture medium, the fermentation temperature is 25 ℃, fermenting for 10 hours, the pH values of the lactic acid bacteria culture medium and the lactic acid bacteria fermentation culture medium are 7, and the lactic acid bacteria culture is obtained after the fermentation is finished, so that the fermented feed has good taste;
the preparation method of the culture solution of the bacillus subtilis comprises the following steps: preparing a bacillus subtilis culture medium from bone soup, wherein the bone soup is prepared from animal bones and water according to the mass ratio of 1:6, then decocting the bone soup with slow fire for 12-24 hours, so that the bacillus subtilis is cultured, inoculating bacillus subtilis strains in the bacillus subtilis culture medium, culturing the bacillus subtilis in an aerobic environment for 60 hours, and culturing the bacillus subtilis at 25 ℃ at a proper temperature to enhance the reproductive capacity of the bacillus subtilis; preparing a culture solution of bacillus subtilis to enhance the digestion gastrointestinal flora of piglets and further promote the digestion capacity of piglets;
as shown in figure 1, the preparation method of the fermented feed comprises the following steps:
s1: grinding:
respectively putting 40 parts of corn and 13 parts of buckwheat into a grinder for grinding, wherein the rotation speed of the grinder is 100r/min, the grinding time is 50min, so that the corn and the buckwheat are quickly powdered, the granularity of the corn flour and the buckwheat is 40 mu m, the granularity is small, the fermentation time of the feed is shortened, the contact area of the feed and the corn flour and the buckwheat is increased, and the corn flour and the buckwheat flour are obtained;
s2: mincing meat:
putting 5 parts of beef into a meat grinder, starting the meat grinder to grind the beef, wherein the rotation speed of the meat grinder is 100min, the meat grinding time is 13min, and obtaining beef extract after meat grinding is finished;
s3: primary mixing:
putting the corn flour, the buckwheat flour and the beef extract obtained in the steps S1 and S2 into a mixer, and then putting 15 parts of bean dregs, 10 parts of fig, 1.8 parts of soybean meal lactobacillus, 1.4 parts of lactobacillus fermentum TLa-6, 0.5 part of acid protease, 0.5 part of neutral protease, 6 parts of lysine, 7 parts of tryptophan, 5 parts of valine, 7 parts of threonine, 1.3 parts of yeast powder, 8 parts of skimmed milk powder, 8 parts of lactose, 0.8 part of beta-carotene, 8 parts of inorganic mineral substances and 3 parts of compound vitamin into the mixer for mixing, wherein the temperature of the mixer is 25 ℃, the rotating speed of the mixer is 70r/min, and the mixing time is 15min to obtain a primary mixed material;
s4 final mixing:
pouring 15 parts of lactobacillus fermentation liquor and 4 parts of bacillus subtilis culture solution into a mixer to be mixed and stirred with the primary mixed material in the step S3, wherein the stirring speed is 90r/min, and the stirring time is 13min, so as to obtain a fermented material;
s5: fermentation:
and pouring the fermented material into a sealed fermentation tank for fermentation, wherein the fermentation time is 70h, the fermentation temperature is 30 ℃, and the fermented feed is obtained after fermentation.
Example 3:
a piglet special high-efficiency utilization type fermented feed for improving immune function comprises the following components in parts by weight: 20 parts of lactobacillus fermentation liquor, 5 parts of bacillus subtilis culture solution, 2.2 parts of soybean meal lactobacillus, 1.5 parts of lactobacillus fermentum TLa-6, 1 part of acid protease, 1 part of neutral protease, 8 parts of lysine, 10 parts of tryptophan, 8 parts of valine, 9 parts of threonine, 2 parts of yeast powder, 10 parts of skimmed milk powder, 9 parts of lactose, 50 parts of corn, 8 parts of beef, 20 parts of bean dregs, 13 parts of fig, 15 parts of buckwheat, 1 part of beta-carotene, 9 parts of composite inorganic mineral substances and 5 parts of composite vitamins;
the composite inorganic mineral comprises the following components in parts by weight: 1.2 parts of ferrous sulfate heptahydrate, 0.9 part of copper sulfate pentahydrate, 0.5 part of zinc sulfate heptahydrate, 1.4 parts of manganese sulfate monohydrate, 1.4 parts of magnesium sulfate, 1.3 parts of sodium chloride, 1.4 parts of potassium dihydrogen sulfate and 0.9 part of calcium dihydrogen phosphate, and the inorganic mineral substances in piglets are supplemented, and the piglet development is promoted;
the compound vitamin comprises the following components in parts by weight: 0.5 part of vitamin A, 0.9 part of vitamin C, 0.8 part of vitamin D, 1.1 part of vitamin E, 0.6 part of vitamin B1, 0.4 part of vitamin B2 and 0.7 part of vitamin B3, and can supplement vitamins in piglets and enhance the immunity of piglets;
the preparation method of the lactic acid bacteria culture solution comprises the following steps: firstly, sterilizing a lactic acid bacteria culture medium at 120 ℃ for 15min, then naturally cooling to room temperature, inoculating a lactic acid bacteria strain on the lactic acid bacteria culture medium, culturing for 1 day, then sterilizing a lactic acid bacteria fermentation culture medium, wherein the sterilization mode is the same as that of the lactic acid bacteria culture medium, then inoculating the lactic acid bacteria culture medium into the lactic acid bacteria fermentation culture medium, the fermentation temperature is 30 ℃, fermenting for 15 hours, the pH values of the lactic acid bacteria culture medium and the lactic acid bacteria fermentation culture medium are 7.5, and the obtained lactic acid bacteria culture medium after fermentation is completed has good mouthfeel of fermented feed;
the preparation method of the culture solution of the bacillus subtilis comprises the following steps: preparing a bacillus subtilis culture medium from bone soup, wherein the bone soup is prepared from animal bones and water according to the mass ratio of 1:6, then decocting the bone soup with slow fire for 12-24 hours, so that the bacillus subtilis is cultured favorably, inoculating bacillus subtilis strains in the bacillus subtilis culture medium, culturing the bacillus subtilis in an aerobic environment for 80 hours, and culturing the bacillus subtilis at the temperature of 30 ℃ at a proper temperature to enhance the reproductive capacity of the bacillus subtilis; preparing a culture solution of bacillus subtilis to enhance the digestion gastrointestinal flora of piglets and further promote the digestion capacity of piglets;
as shown in figure 1, the preparation method of the fermented feed comprises the following steps:
s1: grinding:
respectively putting 50 parts of corn and 15 parts of buckwheat into a grinder for grinding, wherein the rotation speed of the grinder is 120r/min, the grinding time is 60min, so that the corn and the buckwheat are quickly powdered, the granularity of the corn flour and the buckwheat is 55 mu m, the granularity is small, the fermentation time of the feed is shortened, the contact area of the feed and the corn flour and the buckwheat is increased, and the corn flour and the buckwheat flour are obtained;
s2: mincing meat:
putting 8 parts of beef into a meat grinder, starting the meat grinder to grind the beef, wherein the rotation speed of the meat grinder is 120min, the meat grinding time is 15min, and after the meat grinding is finished, beef extract is obtained;
s3: primary mixing:
putting the corn flour, the buckwheat flour and the beef extract obtained in the steps S1 and S2 into a mixer, and then putting 20 parts of bean dregs, 13 parts of fig, 2.2 parts of soybean meal lactobacillus, 1.5 parts of lactobacillus fermentum TLa-6, 1 part of acid protease, 1 part of neutral protease, 8 parts of lysine, 10 parts of tryptophan, 8 parts of valine, 9 parts of threonine, 2 parts of yeast powder, 10 parts of skimmed milk powder, 9 parts of lactose, 1 part of beta-carotene, 9 parts of inorganic mineral substances and 5 parts of compound vitamin into the mixer for mixing, wherein the temperature of the mixer is 30 ℃, the rotating speed of the mixer is 80r/min, and the mixing time is 20min to obtain a primary mixed material;
s4 final mixing:
pouring 20 parts of lactobacillus fermentation liquor and 5 parts of bacillus subtilis culture solution into a mixer to be mixed and stirred with the primary mixed material in the step S3, wherein the stirring speed is 100r/min, and the stirring time is 15min, so as to obtain a fermented material;
s5: fermentation:
and pouring the fermented material into a sealed fermentation tank for fermentation, wherein the fermentation time is 80h, the fermentation temperature is 35 ℃, and the fermented feed is obtained after fermentation.
Claims (10)
1. The efficient utilization type fermented feed special for piglets and capable of improving immune function is characterized by comprising the following components in parts by weight: 10-20 parts of lactobacillus fermentation liquor, 3-5 parts of bacillus subtilis culture solution, 1.2-2.2 parts of soybean meal lactobacillus, 1.3-1.5 parts of lactobacillus fermentum TLa-6, 0.1-1 part of acid protease, 0.1-1 part of neutral protease, 5-8 parts of lysine, 2-10 parts of tryptophan, 2-8 parts of valine, 5-9 parts of threonine, 0.5-2 parts of yeast powder, 5-10 parts of skim milk powder, 7-9 parts of lactose, 30-50 parts of corn, 3-8 parts of beef, 10-20 parts of bean dregs, 8-13 parts of fig, 10-15 parts of buckwheat, 0.3-1 part of beta-carotene, 5-9 parts of compound inorganic mineral substances and 1-5 parts of compound vitamins.
2. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 1, is characterized in that the preparation method of the fermented feed comprises the following steps:
s1: grinding:
respectively grinding 30-50 parts of corn and 10-15 parts of buckwheat in a grinder to obtain corn flour and buckwheat flour;
s2: mincing meat:
putting 3-8 parts of beef into a meat grinder, starting the meat grinder to grind the beef, wherein the rotation speed of the meat grinder is 80-120min, the meat grinding time is 10-15min, and after the meat grinding is finished, beef extract is obtained;
s3: primary mixing:
putting the corn flour, the buckwheat flour and the beef extract obtained in the steps S1 and S2 into a mixer, taking 10-20 parts of bean dregs, 8-13 parts of fig, 1.2-2.2 parts of soybean meal lactobacillus, 1.3-1.5 parts of lactobacillus fermentum TLa-6, 0.1-1 part of acid protease, 0.1-1 part of neutral protease, 5-8 parts of lysine, 2-10 parts of tryptophan, 2-8 parts of valine, 5-9 parts of threonine, 0.5-2 parts of yeast powder, 5-10 parts of skimmed milk powder, 7-9 parts of lactose, 0.3-1 part of beta-carotene, 5-9 parts of inorganic mineral substances and 1-5 parts of compound vitamin, mixing at the mixer temperature of 20-30 ℃, the mixer rotating speed of 60-80r/min for 10-20min, obtaining a primary mixed material;
s4 final mixing:
pouring 10-20 parts of lactobacillus fermentation liquor and 3-5 parts of bacillus subtilis culture solution into a mixer to be mixed with the primary mixed material in the step S3, and stirring at the speed of 70-100r/min for 10-15min to obtain a fermentation material;
s5: fermentation:
and pouring the fermented material into a sealed fermentation tank for fermentation for 50-80h at 25-35 ℃ to obtain the fermented feed.
3. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 1, is characterized in that the composite inorganic mineral comprises the following components in parts by weight: 0.4-1.2 parts of ferrous sulfate heptahydrate, 0.6-0.9 part of copper sulfate pentahydrate, 0.3-0.5 part of zinc sulfate heptahydrate, 0.8-1.4 parts of manganese sulfate monohydrate, 1.1-1.4 parts of magnesium sulfate, 0.8-1.3 parts of sodium chloride, 0.5-1.4 parts of potassium dihydrogen sulfate and 0.5-0.9 part of calcium dihydrogen phosphate.
4. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 1, is characterized in that the vitamin complex comprises the following components in parts by weight: 0.1-0.5 part of vitamin A, 0.2-0.9 part of vitamin C, 0.2-0.8 part of vitamin D, 0.2-1.1 part of vitamin E, 0.1-0.6 part of vitamin B1, 0.1-0.4 part of vitamin B2 and 0.1-0.7 part of vitamin B3.
5. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 1, is characterized in that the preparation method of the lactic acid bacteria culture solution comprises the following steps: firstly, sterilizing a lactic acid bacteria culture medium at the temperature of 100-120 ℃ for 10-15min, then naturally cooling to room temperature, inoculating a lactic acid bacteria strain on the lactic acid bacteria culture medium for culturing for 1 day, then sterilizing a lactic acid bacteria fermentation culture medium in the same way as the lactic acid bacteria culture medium, then inoculating the lactic acid bacteria culture medium into the lactic acid bacteria fermentation culture medium, fermenting at the temperature of 20-30 ℃ for 8-15h, and finally obtaining the lactic acid bacteria culture after fermentation.
6. The efficiently-utilized fermented feed special for piglets, which is according to claim 5, is characterized in that the pH of the lactic acid bacteria culture medium and the lactic acid bacteria fermentation culture medium is 6.5-7.5.
7. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 1, is characterized in that the preparation method of the bacillus subtilis culture solution comprises the following steps: preparing a bacillus subtilis culture medium from the bone soup, inoculating bacillus subtilis strains in the bacillus subtilis culture medium, and culturing in an aerobic environment for 40-80h to prepare a bacillus subtilis culture solution.
8. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 7, is characterized in that the bone soup is made of animal bones, the bone and water are prepared according to the mass ratio of 1:6, and then the mixture is decocted for 12-24 hours with soft fire.
9. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 7, wherein the culture temperature of the bacillus subtilis is 20-30 ℃.
10. The efficient utilization type fermented feed special for piglets, which is used for improving the immune function, according to claim 7, is characterized in that the bone soup is prepared from animal bones and the bone and water are prepared according to a mass ratio of 1: 6.
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| CN115462441A (en) * | 2022-09-20 | 2022-12-13 | 湖南九鼎科技（集团）有限公司 | Wet bean dreg fermented feed and preparation method thereof |
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