CN113337483A - Biological catalase preparation, preparation method thereof and application of biological catalase preparation in removing formaldehyde and peculiar smell - Google Patents

Biological catalase preparation, preparation method thereof and application of biological catalase preparation in removing formaldehyde and peculiar smell Download PDF

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CN113337483A
CN113337483A CN202110615570.1A CN202110615570A CN113337483A CN 113337483 A CN113337483 A CN 113337483A CN 202110615570 A CN202110615570 A CN 202110615570A CN 113337483 A CN113337483 A CN 113337483A
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王冕
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Henan Jingran Environmental Protection Technology Co ltd
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Abstract

The invention discloses a biological catalase preparation, a preparation method thereof and application thereof in removing formaldehyde and peculiar smell, belonging to the technical field of biological catalase, and comprising the following steps: (1) preparing biological enzyme; (2) preparing a carrier; (3) and (5) preparing a finished product. The application provides a biological catalase preparation, a preparation method thereof and application of the biological catalase preparation in formaldehyde and odor removal.

Description

Biological catalase preparation, preparation method thereof and application of biological catalase preparation in removing formaldehyde and peculiar smell
Technical Field
The invention belongs to the technical field of biological enzyme contact agents, and particularly relates to a biological enzyme contact agent, a preparation method thereof and application thereof in removing formaldehyde and peculiar smell.
Background
Formaldehyde is an important chemical raw material and an organic solvent. It is used for producing resin, plastic, medicine, paint, synthetic fiber, adhesive, etc. and may be also used in disinfection, anticorrosion, etc. The formaldehyde in the environment is mainly generated by the combustion of organic substances and various natural and human activities, and can also be generated by the photochemical reaction of volatile organic compounds in the air. Formaldehyde is a highly toxic protoplasm poison which destroys proteins in biological cells, and can bind to amino groups of proteins to denature and coagulate the proteins.
The method for removing formaldehyde by using biotechnology is an environment-friendly, safe and reliable mode, but the research on the method is rarely reported nowadays, and the method is still immature in technology, complex in multiple processes and not remarkable in effect. If the application number is: CN201611128609.2 discloses a biological catalase preparation, a preparation method thereof and application thereof in removing formaldehyde and peculiar smell. The invention provides a biological catalase preparation, which is prepared by mixing a mixed concentrated crude enzyme extract, a soybean amino acid extracting solution and a dilution protective agent according to the volume ratio of 2-5: 1-5: 100; the mixed crude enzyme extract is prepared by mixing a streptococcus lactis concentrated crude enzyme extract, a streptococcus thermophilus concentrated crude enzyme extract and a lactobacillus reuteri concentrated crude enzyme extract according to the proportion of 1:1: 1. The invention also provides a preparation method of the biological catalase preparation and application of the biological catalase preparation in removing formaldehyde and peculiar smell. The invention can remove peculiar smell such as formaldehyde released by decorative materials, furniture, carpets, curtain fabrics and the like and ammonia released by domestic garbage, and improve the environmental quality; the adopted metabolites of the microorganisms and the amino acid extract of the soybean can prevent the secondary pollution of the microorganisms to the living environment, and the soybean raw material has low cost and easy extraction of the amino acid components; the product of the invention is proved to be safe and nontoxic by a third-party experiment. When the biological enzyme preparation is prepared, the preparation method needs to be carried out in the modes of bacterial liquid activation, microbial culture, continuous fermentation, purification and the like, each period is longer, the operation is complicated, and the technical personnel in the field know that the requirements of the bacterial liquid activation and the microbial inoculation culture on the environment are higher, the bacterial liquid activation and the microbial inoculation culture need to be carried out in a sterile environment, and the bacterial contamination can occur once the operation is improper, so that the production rate of the product is reduced. In addition, the enzyme preparation in the application can be inactivated to some extent when meeting a severe environment, so that the activity is reduced, and the formaldehyde removal effect is correspondingly reduced.
Disclosure of Invention
The invention aims to solve the existing problems and provides a biological catalase preparation, a preparation method thereof and application thereof in removing formaldehyde and peculiar smell.
The invention is realized by the following technical scheme:
a method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing soybean protein into a solution with the mass concentration of 15-25%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, and stirring and hydrolyzing by using a stirrer;
(2) preparation of the carrier:
A. placing the sepiolite into a corona discharge instrument for corona treatment, and taking out for later use after the corona treatment is finished;
B. placing the loess stones in an ultramicro pulverizer for pulverizing, and then placing the pulverized loess stones on flame for processing for 1-1.3 min to obtain loess stones for later use;
C. spraying the loess stones obtained in the operation B on the surface of the sepiolite powder obtained in the operation A, and performing laser shock wave treatment while spraying to obtain a compound A for later use;
D. immersing the compound A obtained in the operation C into the treatment fluid, and placing the compound A into a vacuum drying oven for vacuum drying treatment after the treatment is finished to obtain a compound B for later use;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in a conical flask according to the weight ratio of 1: 2-3, and then putting the conical flask in a shaking table for shaking and uniformly mixing;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion.
Further, the wavelength of the light wave is controlled to be 200-500 nm and the frequency of the sound wave is controlled to be 5-10 kHz during the optical-acoustic coupling treatment in the step (1) and B.
Further, the hydrolytic agent in the operation B of the step (1) is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid.
By adopting the technical scheme, the biological enzyme is prepared by an acid method, and more active groups are formed by means of the coupling effect of light waves and sound waves, so that the amino nitrogen content of the soybean protein is increased.
Further, in the step (2), the operation A controls the working voltage to be 10-14 kV during the corona treatment, and the time of the corona treatment is 10-20 s.
Further, controlling the laser shock power density to be 3-4 GW/cm during the laser shock wave processing in the operation C of the step (2)2The energy is 3-5J.
Further, the treating fluid in the operation D of the step (2) comprises the following components in percentage by weight: 0.4-0.8% of linalool, 1-2% of caryophyllene, 0.5-0.9% of hyperin, 10-16% of glycerol, 3-5% of germacrene and the balance of pure water.
Further, the vacuum degree is controlled to be 1.33 to 1.41Pa and the drying temperature is controlled to be 50 to 60 ℃ during the vacuum drying treatment in the operation D of the step (2).
By adopting the technical scheme, the sepiolite can absorb a great amount of water when meeting water to become soft and become hard once being dried, the applicant places the sepiolite in a corona discharge instrument for corona treatment on the basis of the characteristics, micro-concave dense holes are formed on the surface of the sepiolite through discharge, the loess stones have the functions of emitting negative ions, emitting far infrared rays, shielding electromagnetic radiation, resisting bacteria and preventing mildew, absorbing and the like, the applicant places the sepiolite on flame for treatment after carrying out ultramicro crushing treatment on the sepiolite to activate the surface of the loess stones, the treated loess stones are sprayed on the surface of the sepiolite with the dense holes under the auxiliary action of laser shock waves, the loess stones are tightly adhered to the surface of the sepiolite and even embedded into the dense holes on the surface of the sepiolite, when the obtained compound is immersed in the treatment liquid, the sepiolite absorbs water and becomes soft, the loess stones are embedded more tightly, and other components in the treatment liquid are absorbed by the compound, so that the performance of the compound is improved, and the characteristics of purification, mildew resistance, adsorption and the like of the compound are enhanced.
Further, the shaking speed in the operation A and the operation B in the step (3) is 90-150 rpm.
Further, in the step (3), the vacuum degree is controlled to be 1.33-1.66 Pa during the drying in the operation B, and the drying temperature is controlled to be 25-31 ℃.
The application of a biological catalase preparation, in particular to the application of the biological catalase preparation in removing formaldehyde and peculiar smell.
By adopting the technical scheme, the biological enzyme, the catalase and the carrier are mixed, and then the vacuum drying is carried out under the vacuum condition, so that the biological photocatalyst preparation with stable performance is obtained while the enzyme activity is ensured, and the biological photocatalyst preparation can be used for removing the peculiar smell of formaldehyde.
Compared with the prior art, the invention has the following advantages:
the application provides a biological catalase preparation, a preparation method thereof and application of the biological catalase preparation in formaldehyde and odor removal.
Drawings
FIG. 1 is a graph comparing the results of formaldehyde scavenging tests using biological enzyme catalysts prepared in various examples of the detailed description of the present application and the control method.
Detailed Description
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing soybean protein into a solution with the mass concentration of 15-25%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 200-500 nm and the frequency of sound waves to be 5-10 kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment for 10-20 s at 10-14 kV, and taking out for later use;
B. placing the loess stones in an ultramicro pulverizer for pulverizing, and then placing the pulverized loess stones on flame for processing for 1-1.3 min to obtain loess stones for later use;
C. spraying the loess stone obtained in the operation B on the surface of the sepiolite powder in the operation A,performing laser shock wave treatment while spraying, and controlling the laser shock power density to be 3-4 GW/cm2And the energy is 3-5J, so as to obtain a compound A for later use;
D. immersing the compound A obtained in the operation C into the treatment fluid, and then placing the compound A into a vacuum drying oven, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.33-1.41 Pa and the temperature is 50-60 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: 0.4-0.8% of linalool, 1-2% of caryophyllene, 0.5-0.9% of hyperin, 10-16% of glycerol, 3-5% of germacrene and the balance of pure water;
(3) and (3) preparing a finished product:
A. putting the biological enzyme and catalase obtained in the step (1) into an erlenmeyer flask together according to the weight ratio of 1: 2-3, and then putting into a shaking table to shake and mix uniformly at the speed of 90-150 rpm;
B. and D, adding the compound B obtained after treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the compound B is completely shaken, wherein the vacuum degree is controlled to be 1.33-1.66 Pa, and the drying temperature is controlled to be 25-31 ℃.
For further explanation of the present invention, reference will now be made to the following specific examples.
Example 1
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 15%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 200nm and the frequency of sound waves to be 5kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment, treating for 10s at 10kV, and taking out for later use;
B. pulverizing loess stone in an ultramicro pulverizer, and treating with flame for 1min to obtain loess stone powder;
C. spraying the loess stone obtained in the operation B on the surface of the sepiolite powder in the operation A, and performing laser shock wave treatment while spraying, wherein the laser shock power density is controlled to be 3GW/cm2The energy is 3J, and a compound A is obtained for standby;
D. immersing the compound A obtained in the operation C into the treatment fluid, putting the compound A into a vacuum drying oven after the treatment, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.33Pa and the temperature is 50 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: linalool 0.4%, caryophyllene 1%, hyperin 0.5%, glycerin 10%, germacrene 3%, and pure water in balance;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in an erlenmeyer flask according to the weight ratio of 1:2, and then putting the erlenmeyer flask in a shaking table to shake and mix uniformly at the speed of 90 rpm;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion, wherein the vacuum degree is controlled to be 1.33Pa, and the drying temperature is controlled to be 25 ℃.
Example 2
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 20%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 350nm and the frequency of sound waves to be 7.5kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment, treating for 15s at 12kV, and taking out for later use;
B. pulverizing loess stone in an ultramicro pulverizer, and treating with flame for 1.15min to obtain loess stone powder;
C. spraying the loess stone obtained in the operation B on the surface of the sepiolite powder in the operation A, and performing laser shock wave treatment while spraying, wherein the laser shock power density is controlled to be 3.5GW/cm2The energy is 4J, and a compound A is obtained for standby;
D. immersing the compound A obtained in the operation C into the treatment fluid, putting the compound A into a vacuum drying oven after the treatment, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.37Pa and the temperature is 55 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: linalool 0.6%, caryophyllene 1.5%, hyperin 0.7%, glycerin 13%, germacrene 4%, and pure water in balance;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in an erlenmeyer flask according to the weight ratio of 1:2.5, and then putting the erlenmeyer flask in a shaking table to shake and mix uniformly at the speed of 120 rpm;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion, wherein the vacuum degree is controlled to be 1.495Pa, and the drying temperature is controlled to be 28 ℃.
Example 3
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 25%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 500nm and the frequency of sound waves to be 10kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment, carrying out 14kV treatment for 20s, and taking out for later use after the treatment is finished;
B. pulverizing loess stone in an ultramicro pulverizer, and treating with flame for 1.3min to obtain loess stone powder;
C. spraying the loess stone obtained in the operation B on the surface of the sepiolite powder in the operation A, and performing laser shock wave treatment while spraying, wherein the laser shock power density is controlled to be 4GW/cm2The energy is 5J, and a compound A is obtained for standby;
D. immersing the compound A obtained in the operation C into the treatment fluid, putting the compound A into a vacuum drying oven after the treatment, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.41Pa and the temperature is 60 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: linalool 0.8%, caryophyllene 2%, hyperin 0.9%, glycerin 16%, germacrene 5%, and pure water in balance;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in an erlenmeyer flask according to the weight ratio of 1:3, and then putting the erlenmeyer flask in a shaking table to shake and mix uniformly at the speed of 150 rpm;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion, wherein the vacuum degree is controlled to be 1.66Pa, and the drying temperature is controlled to be 31 ℃.
Example 4
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 20%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment, treating for 15s at 12kV, and taking out for later use;
B. pulverizing loess stone in an ultramicro pulverizer, and treating with flame for 1.15min to obtain loess stone powder;
C. will operate in BSpraying the obtained loess stone onto the surface of sepiolite powder in operation A, performing laser shock wave treatment while spraying, and controlling laser shock power density to 3.5GW/cm2The energy is 4J, and a compound A is obtained for standby;
D. immersing the compound A obtained in the operation C into the treatment fluid, putting the compound A into a vacuum drying oven after the treatment, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.37Pa and the temperature is 55 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: linalool 0.6%, caryophyllene 1.5%, hyperin 0.7%, glycerin 13%, germacrene 4%, and pure water in balance;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in an erlenmeyer flask according to the weight ratio of 1:2.5, and then putting the erlenmeyer flask in a shaking table to shake and mix uniformly at the speed of 120 rpm;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion, wherein the vacuum degree is controlled to be 1.495Pa, and the drying temperature is controlled to be 28 ℃.
Example 5
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 20%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 350nm and the frequency of sound waves to be 7.5kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment, treating for 15s at 12kV, and taking out for later use;
B. pulverizing loess stone in an ultramicro pulverizer, and treating with flame for 1.15min to obtain loess stone powder;
C. will be obtained in operation BSpraying loess stone on the surface of sepiolite powder in operation A, and performing laser shock wave treatment while spraying, wherein the laser shock power density is controlled to 3.5GW/cm2The energy is 4J, and a compound A is obtained for standby;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in an erlenmeyer flask according to the weight ratio of 1:2.5, and then putting the erlenmeyer flask in a shaking table to shake and mix uniformly at the speed of 120 rpm;
B. and D, adding the compound A treated in the operation D into the conical flask in the operation A, continuously shaking, and drying in a vacuum drying oven after the compound A is completely shaken, wherein the vacuum degree is controlled to be 1.495Pa, and the drying temperature is controlled to be 28 ℃.
Example 6
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 20%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 350nm and the frequency of sound waves to be 7.5kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) preparation of the carrier:
immersing the sepiolite into the treatment liquid, placing the sepiolite into a vacuum drying oven after the treatment, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.37Pa and the temperature is 55 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: linalool 0.6%, caryophyllene 1.5%, hyperin 0.7%, glycerin 13%, germacrene 4%, and pure water in balance;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in an erlenmeyer flask according to the weight ratio of 1:2.5, and then putting the erlenmeyer flask in a shaking table to shake and mix uniformly at the speed of 120 rpm;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion, wherein the vacuum degree is controlled to be 1.495Pa, and the drying temperature is controlled to be 28 ℃.
Example 7
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of the carrier:
A. placing the sepiolite in a corona discharge instrument for corona treatment, treating for 15s at 12kV, and taking out for later use;
B. pulverizing loess stone in an ultramicro pulverizer, and treating with flame for 1.15min to obtain loess stone powder;
C. spraying the loess stone obtained in the operation B on the surface of the sepiolite powder in the operation A, and performing laser shock wave treatment while spraying, wherein the laser shock power density is controlled to be 3.5GW/cm2The energy is 4J, and a compound A is obtained for standby;
D. immersing the compound A obtained in the operation C into the treatment fluid, putting the compound A into a vacuum drying oven after the treatment, and carrying out vacuum drying treatment under the conditions that the vacuum degree is 1.37Pa and the temperature is 55 ℃ to obtain a compound B for later use; the treating fluid comprises the following components in percentage by weight: linalool 0.6%, caryophyllene 1.5%, hyperin 0.7%, glycerin 13%, germacrene 4%, and pure water in balance;
(2) and (3) preparing a finished product:
A. placing catalase in an Erlenmeyer flask, then placing in a shaker at a speed of 120 rpm;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion, wherein the vacuum degree is controlled to be 1.495Pa, and the drying temperature is controlled to be 28 ℃.
Example 8
A method for preparing a biocatalytic enzyme preparation comprises the following steps:
(1) preparation of biological enzyme:
A. preparing the soybean protein into a solution with the mass concentration of 20%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, controlling the wavelength of light waves to be 350nm and the frequency of sound waves to be 7.5kHz, and stirring and hydrolyzing by using a stirrer, wherein the hydrolytic agent is one of sulfuric acid, hydrochloric acid, citric acid and acetic acid;
(2) and (3) preparing a finished product:
putting the biological enzyme and catalase obtained in the step (1) into an erlenmeyer flask together according to the weight ratio of 1:2.5, then putting the erlenmeyer flask into a shaking table, shaking the erlenmeyer flask at the speed of 120rpm, uniformly mixing the enzymes, and putting the erlenmeyer flask into a vacuum drying oven to dry the substances after the shaking is finished, wherein the vacuum degree is controlled to be 1.495Pa, and the drying temperature is controlled to be 28 ℃.
Control group
The application numbers are: CN201611128609.2 discloses a biological catalase preparation, a preparation method thereof and application thereof in removing formaldehyde and peculiar smell.
In order to compare the technical effects of the application, the methods of the above examples 2, 4 to 8 and the control group are respectively used for preparing the bio-catalase correspondingly, and then the bio-catalase is weighed for standby application, wherein the weighing amount of the bio-catalase in the examples 2 and 4 to 6 is based on the catalase with the same mass, the weighing amount of the bio-catalase in the control group is the same as that in the example 2, then 20mL of formaldehyde solution is weighed into a conical flask, the weighed bio-catalase is soaked into the formaldehyde solution, the conical flask is placed in the dark, and the absorbance is measured every 24 hours, so that the adsorption and removal effects of formaldehyde are examined.
Specific experimental comparative data are shown in figure 1.
As can be seen from figure 1, the application provides a biological catalase preparation, a preparation method thereof and application thereof in formaldehyde and odor removal, the biological catalase preparation and catalase are matched for use, and the finally prepared biological catalase preparation has a remarkable formaldehyde removing effect by virtue of the special carrier effect.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the present invention is not limited to the illustrated embodiments, and all the modifications and equivalents of the embodiments may be made without departing from the spirit of the present invention.

Claims (10)

1. A preparation method of a biocatalytic enzyme preparation is characterized by comprising the following steps:
(1) preparation of biological enzyme:
A. preparing soybean protein into a solution with the mass concentration of 15-25%, and then heating to swell the soybean protein;
B. adding a hydrolytic agent under the condition of light-sound coupling, and stirring and hydrolyzing by using a stirrer;
(2) preparation of the carrier:
A. placing the sepiolite into a corona discharge instrument for corona treatment, and taking out for later use after the corona treatment is finished;
B. placing the loess stones in an ultramicro pulverizer for pulverizing, and then placing the pulverized loess stones on flame for processing for 1-1.3 min to obtain loess stones for later use;
C. spraying the loess stones obtained in the operation B on the surface of the sepiolite powder obtained in the operation A, and performing laser shock wave treatment while spraying to obtain a compound A for later use;
D. immersing the compound A obtained in the operation C into the treatment fluid, and placing the compound A into a vacuum drying oven for vacuum drying treatment after the treatment is finished to obtain a compound B for later use;
(3) and (3) preparing a finished product:
A. putting the biological enzyme obtained in the step (1) and catalase together in a conical flask according to the weight ratio of 1: 2-3, and then putting the conical flask in a shaking table for shaking and uniformly mixing;
B. and D, adding the compound B obtained after the treatment in the operation D into the conical flask obtained in the operation A, continuously shaking, and drying in a vacuum drying oven after the completion.
2. The method for preparing biocatalytic preparation according to claim 1, wherein the wavelength of light wave is controlled to be 200-500 nm and the frequency of sound wave is controlled to be 5-10 kHz in the photo-acoustic coupling treatment in the step (1) operation B.
3. The method of claim 1, wherein said hydrolysis agent in step (1), step B, is selected from the group consisting of sulfuric acid, hydrochloric acid, citric acid, and acetic acid.
4. The method for preparing biocatalytic preparation according to claim 1, wherein the operating voltage of corona treatment in operation A of step (2) is controlled to be 10-14 kV, and the corona treatment time is 10-20 s.
5. The method for preparing biocatalytic preparations according to claim 1, wherein the laser shock power density is controlled to be 3-4 GW/cm during the laser shock wave treatment in the operation C of the step (2)2The energy is 3-5J.
6. The method for preparing biocatalytic preparation according to claim 1, wherein the treating solution in operation D of step (2) comprises the following components in percentage by weight: 0.4-0.8% of linalool, 1-2% of caryophyllene, 0.5-0.9% of hyperin, 10-16% of glycerol, 3-5% of germacrene and the balance of pure water.
7. The method for preparing biocatalytic preparation according to claim 1, wherein the vacuum degree of the vacuum drying treatment in operation D of step (2) is controlled to be 1.33 to 1.41Pa, and the drying temperature is 50 to 60 ℃.
8. The method for preparing a biocatalytic enzyme preparation according to claim 1, wherein the shaking speed in operation A and operation B in step (3) is 90-150 rpm.
9. The method for preparing biocatalytic preparation according to claim 1, wherein the degree of vacuum is controlled to be 1.33 to 1.66Pa and the drying temperature is controlled to be 25 to 31 ℃ in the drying in the operation B of the step (3).
10. The application of the biological catalase preparation is characterized in that the biological catalase preparation is particularly applied to removing formaldehyde and peculiar smell.
CN202110615570.1A 2021-06-02 2021-06-02 Biological catalase preparation, preparation method thereof and application of biological catalase preparation in removing formaldehyde and peculiar smell Pending CN113337483A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114806670A (en) * 2022-04-27 2022-07-29 北京华业中科科技发展有限公司 Energy-saving and environment-friendly catalyst for coal combustion

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104645819A (en) * 2013-11-21 2015-05-27 马啸天 Production process of soybean protein formaldehyde removing agent
CN106731769A (en) * 2016-12-09 2017-05-31 大连康帕仕环境科技有限公司 Biological catalase preparation and preparation method thereof and the application in being eliminated the unusual smell except formaldehyde
CN107802978A (en) * 2017-10-31 2018-03-16 山东德慧环境科技有限公司 A kind of soybean protein formaldehyde scavenger and its production technology
CN108479721A (en) * 2018-05-02 2018-09-04 江苏省农业科学院 A kind of methyl aldehyde adsorption material and its application
CN109731461A (en) * 2019-02-18 2019-05-10 浙江健煦环境科技有限公司 A kind of biological enzyme formaldehyde scavenger and preparation method thereof
CN110227340A (en) * 2019-06-24 2019-09-13 关圣睿 A kind of removal formaldehyde composite bacteria agent and its preparation method and application

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104645819A (en) * 2013-11-21 2015-05-27 马啸天 Production process of soybean protein formaldehyde removing agent
CN106731769A (en) * 2016-12-09 2017-05-31 大连康帕仕环境科技有限公司 Biological catalase preparation and preparation method thereof and the application in being eliminated the unusual smell except formaldehyde
CN107802978A (en) * 2017-10-31 2018-03-16 山东德慧环境科技有限公司 A kind of soybean protein formaldehyde scavenger and its production technology
CN108479721A (en) * 2018-05-02 2018-09-04 江苏省农业科学院 A kind of methyl aldehyde adsorption material and its application
CN109731461A (en) * 2019-02-18 2019-05-10 浙江健煦环境科技有限公司 A kind of biological enzyme formaldehyde scavenger and preparation method thereof
CN110227340A (en) * 2019-06-24 2019-09-13 关圣睿 A kind of removal formaldehyde composite bacteria agent and its preparation method and application

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
刘宝成 等: "活性炭吸附去除室内甲醛的研究进展", 成都纺织高等专科学校学报, no. 1, pages 224 - 229 *
左红梅等: "生物酶/ACF复合材料降解甲醛实验研究", 化工新型材料, vol. 41, no. 7, pages 115 - 116 *
熊祥等: "浅谈除臭技术在猪场的应用研究", 现代农业装备, no. 6, pages 37 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114806670A (en) * 2022-04-27 2022-07-29 北京华业中科科技发展有限公司 Energy-saving and environment-friendly catalyst for coal combustion

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