CN113332460A - Ultraviolet C wave treatment method for vitamin C-containing solution - Google Patents
Ultraviolet C wave treatment method for vitamin C-containing solution Download PDFInfo
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- CN113332460A CN113332460A CN202110339467.9A CN202110339467A CN113332460A CN 113332460 A CN113332460 A CN 113332460A CN 202110339467 A CN202110339467 A CN 202110339467A CN 113332460 A CN113332460 A CN 113332460A
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- ultraviolet
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- wave treatment
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title claims abstract description 86
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 229930003268 Vitamin C Natural products 0.000 title claims abstract description 43
- 235000019154 vitamin C Nutrition 0.000 title claims abstract description 43
- 239000011718 vitamin C Substances 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000001678 irradiating effect Effects 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 83
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 3
- 230000003595 spectral effect Effects 0.000 abstract 1
- 238000002560 therapeutic procedure Methods 0.000 description 6
- 239000012488 sample solution Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 230000007704 transition Effects 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000486463 Eugraphe sigma Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000005610 quantum mechanics Effects 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Radiation-Therapy Devices (AREA)
Abstract
The invention discloses an ultraviolet C wave treatment method of a vitamin C-containing solution, which comprises the following steps: the method comprises the steps of continuously irradiating a solution containing vitamin C for a certain time by adopting ultraviolet rays, adjusting the specific wavelength of an ultraviolet C wave according to the spectral absorption peak of the vitamin C, enabling the original electron in the microscopic form to transit to an unstable high-energy orbit after the vitamin C is absorbed, returning to the original energy orbit after a certain time, and releasing the photon with specific energy absorbed by the vitamin C at the stage. The product obtained by specially processing the vitamin C solution can release ultraviolet C wave photons, and has a breakthrough effect in the medical field.
Description
Technical Field
The invention relates to the technical field of ultraviolet rays, in particular to an ultraviolet C wave treatment method for a vitamin C-containing solution.
Background
Ultraviolet photon blood therapy (UBIO therapy), a therapeutic method in which blood is extracted from the body, irradiated with ultraviolet rays at regular time and quantity, and then returned to the body, originated in the european and american countries in the last 20 th century, has been widely used abroad after more than 80 years of clinical application and development, and has been used in China for more than 30 years. For example, cn92226208.x, describes an apparatus for irradiating ex vivo blood with ultraviolet light.
However, the therapy has been controversial since the advent, and in particular, certain damage to the human body can be caused by blood being drawn out of the body and then returned to the human body. The latter ultraviolet internal therapy, for example, CN109414591A, describes an internal ultraviolet therapy in which an ultraviolet light emitting device is introduced into a human body through a delivery tube, an endoscope, or the like, but this method causes ultraviolet light to be directly emitted into the human body, which causes great damage to the human body.
Disclosure of Invention
The invention aims to provide an ultraviolet C wave treatment method of a vitamin C-containing solution and an ultraviolet C wave treatment instrument.
The technical scheme of the invention is as follows: a method for treating vitamin C-containing solution by ultraviolet C wave,
step 1: starting an ultraviolet generating device to irradiate a solution pipeline, wherein the solution pipeline is provided with at least one inlet and at least one outlet;
step 2: introducing a solution I into an inlet of a solution pipeline, wherein the solution I contains vitamin C;
and step 3: keeping the ultraviolet generator continuously irradiating, keeping the solution I continuously flowing in from the solution pipeline inlet, and keeping the irradiated solution I flowing out from the solution pipeline outlet for 1-2 hours.
In the above method for processing vitamin C-containing solution by using ultraviolet C waves, the ultraviolet generating module generates ultraviolet C waves with a wavelength of 100nm to 280 nm.
In the method for processing ultraviolet C wave containing vitamin C solution, the ultraviolet generating module generates ultraviolet C wave with a wavelength of 245 nm.
In the method for treating vitamin C-containing solution by using ultraviolet C waves, the solution I is based on normal saline or 0.9% glucose solution or a mixture of the normal saline and the glucose solution.
In the method for treating the vitamin C-containing solution by using the ultraviolet C wave, the flow rate of the solution I flowing into and out of the solution pipeline is 1-5 ml/min.
In the method for treating the vitamin C-containing solution by using the ultraviolet C wave, in step 3, after the solution I starts to flow into the inlet of the solution pipeline for 0.5 hour, the inflow rate of the solution I is reduced by 20% -50%.
In the method for treating the vitamin C-containing solution by using the ultraviolet C waves, the ultraviolet generating device is located inside the solution pipeline, and a quartz glass tube is used at the periphery of the ultraviolet generating device.
In the method for treating the vitamin C-containing solution by using the ultraviolet C wave, the intensity of the ultraviolet generated by the ultraviolet generating module is 3 to 11.
In the method for treating the vitamin C-containing solution by using the ultraviolet C wave, the content of the vitamin C in the solution I is 6-18 g/L.
The supporting equipment for the ultraviolet C wave treatment method of the vitamin C-containing solution comprises a box-type device surrounded by a surrounding plate, wherein the box-type device is provided with a detachable box cover, an ultraviolet generating device is installed in the box-type device, a glass tube is arranged between the box cover and the ultraviolet generating device, the glass tube is fixed on the surrounding plate, and the output end and the input end of the glass tube are exposed out of the surrounding plate.
The invention has the advantages that: the vitamin C-containing solution has the ultraviolet sterilization effect after being initiatively subjected to the ultraviolet irradiation effect, can be used for sterilization of special positions, and has a very high medical promotion prospect.
Description of the drawings:
fig. 1 is a schematic illustration of the apparatus mentioned in the examples.
Detailed Description
The invention is further illustrated by the following figures and examples, which are not to be construed as limiting the invention.
Example (b): comprising a device, as shown with reference to fig. 1: comprises a box-type device enclosed by a coaming 2, wherein the box-type device is provided with a detachable box cover 1, and the box cover 1 is arranged to be detachable
The ultraviolet generating device 21 is installed in the box-type device, the quartz glass tube 3 is arranged between the box cover 1 and the ultraviolet generating device 21, the quartz glass tube 3 is fixed on the enclosing plate 2, and the output end and the input end of the quartz glass tube 3 are exposed out of the enclosing plate 2.
The equipment is used for treating the ultraviolet C wave containing vitamin C solution, and comprises the following specific steps:
step 1: starting an ultraviolet generating device to irradiate the quartz glass tube; the ultraviolet generating device emits ultraviolet C wave with wavelength of 100-280nm, and the intensity of the ultraviolet generated by the ultraviolet generating module is 3-11 of the ultraviolet index.
Step 2: introducing a solution I into an inlet of a quartz glass tube, wherein the solution I is based on normal saline or 5% glucose solution or a mixture of the normal saline and the 5% glucose solution, and the solution I contains vitamin C;
and step 3: keeping the ultraviolet generator continuously irradiating, keeping the solution I continuously flowing in from the inlet of the solution pipeline, keeping the irradiated solution I flowing out from the outlet of the solution pipeline, keeping the duration for 1-2 hours, and keeping the flow rate of the solution I flowing into and out of the solution pipeline to be 1-5 ml/min;
and the inflow rate of the solution I is reduced by 20 to 50 percent after the solution I starts flowing into the inlet of the solution pipeline for 0.5 hour.
Based on the implementation of the above example, the principle of the ultraviolet treatment of the vitamin C-containing solution is as follows: through the specific reaction of vitamin C and ultraviolet C wave, the light quantum is brought into a specific position to produce the effect.
Molecules of vitamin C:
wherein, the hydroxyl of the functional group connected with the C2 and C3 atoms is unstable, the electron is more active, and the electron is transited under the action of absorbing ultraviolet irradiation and a magnetic field; according to the basic rules of quantum mechanics, when the energy of a photon is just equal to the energy difference between the two energy levels at which the transition occurs, and after the transition, the electron is unstable, it will return from the high-level orbital to the low-level orbital at a certain time without being too long, releasing the photon.
The ultraviolet absorption of organic compound has three kinds of electrons, i.e. sigma electron, pi electron and n electron, and there are three kinds of electron orbitals, bonding orbit, non-bonding orbit and reverse bonding orbit. Wherein the energy state of the bonding orbit is lowest, the energy state of the non-bonding orbit is next to that of the bonding orbit, and the energy state of the anti-bonding orbit is highest.
The active electron on the hydroxyl group of C2 in vitamin C starts at the bonding orbital, and when receiving a photon, it transits to the non-bonding orbital or the anti-bonding orbital, and the optical quantum wavelength at which the electron transits is 245nm depending on E ═ hc/λ. That is, the absorption of a 245nm photon by a hydrogen atom causes its electron transition and the energy state increases, while the electron transition back to the low energy state orbit releases a photon of 245nm wavelength.
According to the ultraviolet segmentation, the ultraviolet C wave belongs to an ultraviolet short wave band, and the wavelength of the ultraviolet C wave is 100-280nm, so that 245nm light is in the wave band. The ultraviolet C wave band is characterized by weak penetrability and strong sterilization capability.
It is worth mentioning here that under perfect conditions (neutral or more basic environment provided in the laboratory) the above mentioned 245nm results;
and by carrying out more experiments on the samples, the following conclusions are drawn: the characteristic wavelength (even the wavelength of atomic transition) of the vitamin C solution is in an interval, and the larger the acidity is, the shorter the wavelength is; the greater the basicity, the longer the wavelength; the interval also corresponds to the wavelength interval of the ultraviolet C wave, namely 100-280 nm.
The vitamin C solution after ultraviolet irradiation treatment can release ultraviolet C-wave photons with the same absorption wavelength;
according to the characteristics, the method can be widely applied to scenes, such as ultraviolet sterilization by introducing parts which cannot be accessed by certain existing equipment, such as pipeline sterilization.
The following are experimental validation sections:
experimental materials and tools: a strain liquid, a sample liquid containing VC (aqueous solution containing 20% of vitamin C), distilled water and a device capable of emitting ultraviolet rays (ultraviolet lamp), a sterile test tube; wherein the strain liquid contains Escherichia coli and Staphylococcus aureus. Specifically, selecting thallus Porphyrae from positive plate, and making into 0.5 McLee's bacteria solution, which is 108cfu/ml strain stock solution. Will 108Diluting cfu/ml strain stock solution to 103cfu/ml, respectively as Escherichia coli solution and Staphylococcus aureus solutionThe use is carried out;
the experimental process comprises the following steps:
the negative control group was set as: introducing the sample solution irradiated by ultraviolet light for 1 hour into distilled water (four groups in total), mixing, labeling, immediately culturing in a sterile working room for 48 hours, observing and counting; the negative control is also called blank control, and is used for testing whether the environment is completely sterile, if the control group is not 0, the environment pollution is indicated when the colony grows, and the experiment fails and needs to be repeated completely.
The positive control group was: introducing sample solution without ultraviolet irradiation into the above bacterial solution (Escherichia coli, Staphylococcus aureus, two groups including 4 rows), mixing, labeling, immediately culturing in sterile working room for 48 hr, observing, and counting;
the sample control groups were: and (3) introducing the sample solution irradiated by ultraviolet light for 1 hour into the bacterial solution (four groups in total), uniformly mixing, preparing a label, immediately conveying the label to a sterile working room for flat culture for 48 hours, and observing and counting.
The positive control group and the sample control group are set for comparison: the influence of the sample solution after ultraviolet irradiation on the number of bacterial colonies in the mixed bacterial solution.
The above groups are all plates which are divided in advance, and are synchronously operated in parallel, and the experimental results are as follows:
the calculation mode of the upper table of the bacteriostatic rate is as follows: s ═ 100% > (positive group mean-whole group mean)/positive group mean. In conclusion, the sample solution after being irradiated by ultraviolet rays obviously has the bacteriostatic function.
Claims (10)
1. A method for treating vitamin C-containing solution by ultraviolet C wave is characterized in that:
step 1: starting an ultraviolet generating device to irradiate a solution pipeline, wherein the solution pipeline is provided with at least one inlet and at least one outlet;
step 2: introducing a solution I into an inlet of a solution pipeline, wherein the solution I contains vitamin C;
and step 3: keeping the ultraviolet generator continuously irradiating, keeping the solution I continuously flowing in from the solution pipeline inlet, and keeping the irradiated solution I flowing out from the solution pipeline outlet for 1-2 hours.
2. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 1, wherein: the ultraviolet generating module generates ultraviolet C wave with the wavelength of 100nm-280 nm.
3. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 2, wherein: the ultraviolet generating module generates ultraviolet C wave with the wavelength of 245 nm.
4. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 1, wherein: the base of the solution I is physiological saline or 5% glucose solution or a mixture of the two.
5. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 1, wherein: the flow rate of the solution I flowing into and out of the solution pipeline is 1-5 ml/min.
6. The method of claim 5, wherein the vitamin C-containing solution is subjected to the ultraviolet C wave treatment, wherein the ultraviolet C wave treatment comprises: in the step 3, after the solution I starts to flow into the inlet of the solution pipeline for 0.5 hour, the inflow flow rate of the solution I is reduced by 20-50%.
7. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 1, wherein: the ultraviolet generating device is positioned in the solution pipeline, and a quartz glass tube is adopted at the periphery of the ultraviolet generating device.
8. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 1, wherein: the intensity of the ultraviolet rays generated by the ultraviolet ray generation module is 3-11 of the ultraviolet ray index.
9. The method for ultraviolet C-wave treatment of a vitamin C-containing solution as set forth in claim 1, wherein: the content of vitamin C in the solution I is 6-18 g/L.
10. The apparatus as claimed in any one of claims 1 to 9, wherein the apparatus comprises: the ultraviolet light box device comprises a box type device surrounded by surrounding plates (2), wherein the box type device is provided with a detachable box cover (1), an ultraviolet light generating device (21) is installed in the box type device, a glass tube (3) is arranged between the box cover (1) and the ultraviolet light generating device (21), the glass tube (3) is fixed on the surrounding plates (2), and the output end and the input end of the glass tube (3) are exposed out of the surrounding plates (2).
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1159955A (en) * | 1996-03-20 | 1997-09-24 | 张志强 | Ultraviolet light quantum intravascular irradiation therapeutic machine |
| CN1287496A (en) * | 1998-07-21 | 2001-03-14 | 甘布洛公司 | Method for inactivation of microorganisms using photosensitizers |
| WO2009149020A1 (en) * | 2008-06-04 | 2009-12-10 | Triton Thalassic Technologies, Inc. | Methods, systems and apparatus for monochromatic uv light sterilization |
| WO2010123993A1 (en) * | 2009-04-21 | 2010-10-28 | Tuan Vo-Dinh | Non-invasive energy upconversion methods and systems for in-situ photobiomodulation |
| CN103328609A (en) * | 2011-01-04 | 2013-09-25 | 皇家飞利浦电子股份有限公司 | UV-emitting phosphors |
| CN104986823A (en) * | 2015-07-01 | 2015-10-21 | 青岛杰生电气有限公司 | Ultraviolet bacteriostatic method and purified direct drinking water bacteriostatic method |
| CN107242867A (en) * | 2017-07-25 | 2017-10-13 | 重庆恩硕利科技有限公司 | A kind of bio wave electronic detector with sterilizing function |
| CN107496950A (en) * | 2017-08-18 | 2017-12-22 | 广州市众为生物技术有限公司 | A kind of viral method in UV irradiation inactivation protein solution |
-
2021
- 2021-03-30 CN CN202110339467.9A patent/CN113332460A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1159955A (en) * | 1996-03-20 | 1997-09-24 | 张志强 | Ultraviolet light quantum intravascular irradiation therapeutic machine |
| CN1287496A (en) * | 1998-07-21 | 2001-03-14 | 甘布洛公司 | Method for inactivation of microorganisms using photosensitizers |
| WO2009149020A1 (en) * | 2008-06-04 | 2009-12-10 | Triton Thalassic Technologies, Inc. | Methods, systems and apparatus for monochromatic uv light sterilization |
| WO2010123993A1 (en) * | 2009-04-21 | 2010-10-28 | Tuan Vo-Dinh | Non-invasive energy upconversion methods and systems for in-situ photobiomodulation |
| CN103328609A (en) * | 2011-01-04 | 2013-09-25 | 皇家飞利浦电子股份有限公司 | UV-emitting phosphors |
| CN104986823A (en) * | 2015-07-01 | 2015-10-21 | 青岛杰生电气有限公司 | Ultraviolet bacteriostatic method and purified direct drinking water bacteriostatic method |
| CN107242867A (en) * | 2017-07-25 | 2017-10-13 | 重庆恩硕利科技有限公司 | A kind of bio wave electronic detector with sterilizing function |
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Non-Patent Citations (1)
| Title |
|---|
| 熊志立主编: "《分析化学》", 31 December 2019, 中国医药科技出版社 * |
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Application publication date: 20210903 |