CN113331317A - Feed for improving growth performance of hermetia illucens - Google Patents

Feed for improving growth performance of hermetia illucens Download PDF

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Publication number
CN113331317A
CN113331317A CN202110582846.0A CN202110582846A CN113331317A CN 113331317 A CN113331317 A CN 113331317A CN 202110582846 A CN202110582846 A CN 202110582846A CN 113331317 A CN113331317 A CN 113331317A
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feed
parts
hermetia illucens
improving
growth performance
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胡德宝
李振阳
郭震
孙嘉伯
丁向彬
郭益文
赵欣雅
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Tianjin Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/111Aromatic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/116Heterocyclic compounds
    • A23K20/121Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a feed for improving the growth performance of hermetia illucens, which comprises the following components in parts by mass: 0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water. According to the feed for improving the growth performance of the hermetia illucens, the soybean oil, the linseed oil, the tert-butyl hydroquinone and the tween-20 are matched, so that the fat nutrition concentration of the feed is improved, the storage time of the feed for improving the growth performance of the hermetia illucens is prolonged, the oxidation rate of the feed is reduced, and the components of the feed for improving the growth performance of the hermetia illucens are mixed more uniformly. Can effectively prolong the preservation time of the feed for improving the growth performance of the hermetia illucens.

Description

Feed for improving growth performance of hermetia illucens
Technical Field
The invention belongs to the technical field of animal feed, relates to feed, and particularly relates to feed for improving the growth performance of hermetia illucens.
Background
Along with the continuous improvement of Chinese economy, the demand of people for meat products is also continuously improved, so that the scale of livestock and poultry breeding production is larger and larger, but the waste treatment and utilization work generated by breeding is relatively lagged. The livestock waste refers to wastes such as livestock manure, livestock house padding, waste feed and scattered hairiness in a breeding farm, and is mostly directly discharged without harmless treatment or dry-wet separation, thereby causing pollution.
The black soldier fly can effectively reduce the accumulation of waste and convert the waste into self nutrient substances of a worm body by recycling the livestock and poultry manure and the kitchen waste, the protein content of larvae of the black soldier fly is high, and the black soldier fly can replace certain protein feed to feed animals, so that the effect of secondary utilization of the waste is achieved, and the economic cost of the feed is also reduced. The black soldier fly is raised simply and easy management, and the required living environment of polypide easily provides, does not need to feed any food in adult raising stage, only need provide drinking water can, only need the amateur time raise in the raising stage of larva can to the food consumption is low during the larva, the food source is extensive, as corrosive insect, various discarded object in the life can both become its food. Meanwhile, the breeding capacity of the insect is high, each time, the egg laying amount is 800-1000, the breeding period is about one month, and the output efficiency and the waste treatment capacity can be greatly improved.
Compared with flies, the hermetia illucens is relatively clean to be cultured, meanwhile, the hermetia illucens cannot enter the human society, and no bacteria or viruses with diseases are carried, researches show that organic matters in excrement can be effectively absorbed by feeding the hermetia illucens by using animal excrement as a raw material, and analysis of research results shows that nitrogen and phosphorus contents in excrement treated by hermetia illucens larvae are obviously reduced, conversion products (insect sand) have no peculiar smell, meanwhile, oviposition behaviors of flies can be effectively prevented, and the number of flies in animal excrement is reduced by more than 90%.
The black soldier fly is used for degrading the livestock and poultry waste, and meanwhile, the black soldier fly is used as the feed, so that the feed is one of the research hotspots in the fields of feed science and environmental protection at present, and the feed nutrition is a key factor for regulating and controlling the growth index and the nutrition index of the black soldier fly. At present, the research results of feed formulas for feeding the hermetia illucens are few, and the feed formulas are difficult to transform, popularize and apply, so that the hermetia illucens feed formula convenient to transform, popularize and apply is found and has important economic value in production practice to improve the growth performance of the hermetia illucens.
Through searching, no patent publication related to the present patent application has been found.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the feed for improving the growth performance of the hermetia illucens.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the feed for improving the growth performance of the hermetia illucens comprises the following components in parts by mass:
0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water.
Further, the composition comprises the following components in parts by weight:
2.4 parts of soybean oil, 3.6 parts of linseed oil, 0.0012 part of tert-butyl hydroquinone, 78 parts of laying hen manure, 42 parts of water and 2% tween-201.68 parts.
Furthermore, the linolenic acid content in the linseed oil is higher and accounts for more than 50% (mass percent) of the total fatty acids.
Further, the linseed oil is added to the feed to a final mass concentration of 2%.
The preparation method of the feed for improving the growth performance of the hermetia illucens comprises the following steps:
s1, adding tert-butyl hydroquinone into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the layer chicken manure to 70% by using water, and then adding 2% of Tween-20;
s3, uniformly mixing the anti-oxidation soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 to obtain feed for improving the growth performance of black soldier flies;
the above operations are all carried out in the environment with the temperature of 18-25 ℃ and the air humidity of less than 25%.
The feed for improving the growth performance of the hermetia illucens is applied to the application of the feed as the feed for the hermetia illucens.
The feed for improving the growth performance of the hermetia illucens is applied to the feed for the hermetia illucens larvae.
The invention has the advantages and positive effects that:
1. according to the feed for improving the growth performance of the hermetia illucens, the soybean oil, the linseed oil, the tert-butyl hydroquinone and the tween-20 are matched, so that the fat nutrition concentration of the feed is improved, the storage time of the feed for improving the growth performance of the hermetia illucens is prolonged, the oxidation rate of the feed is reduced, and the components of the feed for improving the growth performance of the hermetia illucens are mixed more uniformly. Can effectively prolong the preservation time of the feed for improving the growth performance of the hermetia illucens.
2. According to the invention, the black soldier fly is fed by using the feed formula of the linseed oil, wherein the 2% of the linseed oil is added, so that the growth performances of hundred worm weight, worm body length, worm body width, crude fat and the like of the black soldier fly larvae can be obviously improved. The feed formula is suitable for breeding the hermetia illucens larvae, the growth performance of the hermetia illucens is improved, and a foundation is laid for transformation, popularization and application of the hermetia illucens.
3. The feed for improving the growth performance of the hermetia illucens comprises the following components:
soybean oil: soybean oil is an oil extracted by pressing from soybeans, is rich in various valuable nutritional ingredients, and contains a large amount of linoleic acid. In animal production, a certain amount of soybean oil is often added to increase the energy value in the feed. ② linseed oil: the linseed oil is prepared from linseed, the content of unsaturated fatty acid in fatty acid of the linseed oil is higher, and the content of alpha-linolenic acid is as high as more than 50%. In addition, the linseed oil also contains rich nutrient components such as vitamin E, sterol and the like, and researches show that the linseed oil in the feed can improve the animal immunity. ③ tween-20: is a surfactant, is an oil-in-water (O/W) emulsifier, and can be used as solubilizer, dispersing agent, stabilizer, antistatic agent, and lubricant.
Tert-butyl hydroquinone: tert-butyl hydroquinone (tBHQ) is a food antioxidant additive, and researches show that the addition of the tBHQ can prevent the rancidity of oil and fat substances and has an antioxidant effect.
Detailed Description
The following detailed description of the embodiments of the present invention is provided for the purpose of illustration and not limitation, and should not be construed as limiting the scope of the invention.
The raw materials used in the invention are conventional commercial products unless otherwise specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The feed for improving the growth performance of the hermetia illucens comprises the following components in parts by mass:
0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water.
Preferably, the composition comprises the following components in parts by weight:
2.4 parts of soybean oil, 3.6 parts of linseed oil, 0.0012 part of tert-butyl hydroquinone, 78 parts of laying hen manure, 42 parts of water and 2% tween-201.68 parts.
Preferably, the linolenic acid content in the linseed oil is higher and accounts for more than 50% (mass percent) of the total fatty acids.
Preferably, the linseed oil is added to the feed to a final mass concentration of 2%.
The preparation method of the feed for improving the growth performance of the hermetia illucens comprises the following steps:
s1, adding tert-butyl hydroquinone into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the layer chicken manure to 70% by using water, and then adding 2% of Tween-20;
s3, uniformly mixing the anti-oxidation soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 to obtain feed for improving the growth performance of black soldier flies;
the above operations are all carried out in the environment with the temperature of 18-25 ℃ and the air humidity of less than 25%.
The feed for improving the growth performance of the hermetia illucens is applied to the application of the feed as the feed for the hermetia illucens.
The feed for improving the growth performance of the hermetia illucens is applied to the feed for the hermetia illucens larvae.
Specifically, the preparation and detection examples are as follows:
examples 1-3 feed for improving growth performance of hermetia illucens
Examples 1 to 3 are feeds for improving the growth performance of black soldier flies, and the composition of raw materials for preparing antioxidant soybean oil linseed oil is shown in table 1, and the composition of raw materials for preparing chicken manure is shown in table 2.
Table 1 examples 1-3 raw materials for antioxidant soybean oil, linseed oil
Figure BDA0003086645210000041
TABLE 2 examples 1-3 preparation of raw materials for chicken manure
Figure BDA0003086645210000042
Examples 1-3 methods for preparing feed for improving the growth performance of hermetia illucens are performed according to the following steps, all of which are performed in an environment with a temperature of 18-25 ℃ and an air humidity of less than 25%, as follows:
s1, adding tert-butyl hydroquinone (200 mu g/g grease) into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the chicken manure to 70% by using water, and adding 2% of Tween-20 which accounts for 2% of the water content of the adjusted chicken manure;
s3, mixing the antioxidant soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 uniformly to obtain the feed for improving the growth performance of the hermetia illucens.
The correlation test of the present invention is as follows:
the verification test of the feed for improving the growth performance of the hermetia illucens comprises the following steps:
(1) test animal selection and grouping
By adopting a complete random design of a single-factor experimental scheme, 2400 black soldier fly larvae with similar hundred-worm weight and strong peristalsis capacity and 1 year old are selected and randomly divided into 3 groups (example 1, example 2 and example 3), each group is divided into 4 replicates, each replicate 200 larvae, fresh chicken manure added with 5% of soybean oil is taken as a control group (example 1), and fresh chicken manure added with linseed oil instead of the soybean oil is taken as a test group respectively (example 2) and 5% (example 3).
(2) Feed and feeding management
The control group was fed with the feed of example 1, and the test group was fed with the test feed supplemented with linseed oil instead of soybean oil (example 2) and 5% (example 3) of the feed for improving the growth performance of hermetia illucens, which were fed freely during the test. A650 ml disposable injection molding meal box was used as a feeding container for hermetia illucens larvae, and a 12cm x 6cm vent was cut out with scissors in the middle of the meal box cover to facilitate the breathing of the hermetia illucens larvae. Adding 1-year larvae and feed for improving the growth performance of hermetia illucens into a disposable injection-molded lunch box, putting the meal box into a biochemical incubator, keeping the temperature at 28 ℃, ventilating and replenishing water every day, wherein the water replenishing amount is based on the aim of replenishing the humidity of the feed to about 70%, the feed is added for multiple times, and the rest 50% of the feed is added as reference.
(3) Collection and index determination
The first blackened larva appearing in the disposable injection molding lunch box is used as a signal, the disposable injection molding lunch box is collected completely at one time, and residual excrement on the surface of the disposable injection molding lunch box is cleaned up by utilizing the larva wriggling characteristic. Starvation treatment is carried out for 24 hours, so that undigested residues in the digestive tract of the polypide are discharged. And then removing initial water in an electrothermal blowing dry box at the temperature of 60-70 ℃ to prepare a semi-dry sample, and measuring the weight of the beetles, the length of the beetles, the width of the beetles, crude protein and crude fat according to the following method.
Measuring the weight of the hundred insects: taking 100 6-th-instar hermetia illucens larvae by a quartering method in each group, and weighing by using an analytical balance;
measuring the length and width of the worm body: taking 50 6 th larvae of hermetia illucens in each group by a quartering method, and measuring the length (the length from head to tail) and the width (the widest distance on two sides of the larvae) of the larvae by using vernier calipers;
measuring the crude protein of the polypide (Kjeldahl method): grinding the air-dried worm bodies into powder by using a mortar, accurately weighing about 0.5g (accurate to 0.1mg) of worm powder into a digestion tube, adding 10ml of concentrated sulfuric acid, digesting for 1h at 420 ℃, and performing full-automatic crude protein analysis by using a SCINOKT260 Kjeldahl azotometer;
fourthly, measuring the crude fat of the worm body (Soxhlet extraction method): the air-dried worm bodies were ground into powder using a mortar, and about 2g (to an accuracy of 0.1mg) of the worm powder was weighed accurately into a filter paper cartridge, and the mass was recorded as m. Weighing the mass of the empty aluminum cup, and recording as m1Placing the filter paper cylinder and the aluminum cup into a Soxtec 8000 Soxhlet extractor for extraction, and weighing the mass of the aluminum cup after extraction is finished, and recording the mass as m2. The worm dry matter has a crude fat content of (m)2-m1)/m。
(4) Test results
TABLE 4 Pest determination results
Initial weight/g Total weight of fresh insect/g Weight of hundred insects/g
Example 1 0.51±0.00 21.88±1.13ab 11.99±0.68ab
Example 2 0.51±0.01 23.00±1.32a 12.10±0.52a
Example 3 0.50±0.01 19.83±1.06b 11.17±0.37b
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As can be seen from Table 4, there is no significant difference in initial insect weights in examples 1, 2 and 3, and test comparability is ensured.
Example 2 significantly increased the total weight and hundred weight of hermetia illucens (P <0.05) compared to example 3.
TABLE 5 measurement results of length and width of worm
Length/cm of insect body Width/cm of insect body
Example 1 1.709±0.115a 0.497±0.037b
Example 2 1.722±0.106a 0.514±0.039a
Example 3 1.640±0.136b 0.507±0.040a
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As shown in Table 5, the length of the black soldier fly larvae was significantly increased (P <0.05) in examples 1 and 2 as compared with example 3, and the width of the black soldier fly larvae was significantly increased (P <0.05) in example 2 as compared with example 1
TABLE 6 measurement results of crude protein and crude fat of polypide
Crude protein (%) Crude fat (%)
Example 1 48.72±2.38a 25.50±2.50b
Example 2 45.99±1.20b 27.53±2.12ab
Example 3 43.31±1.25b 31.34±2.76a
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As shown in table 6, in examples 2 and 3, the crude fat content of the black soldier fly larvae was significantly increased (P <0.05) as compared to example 1; in example 1, the crude protein content was significantly increased (P <0.05) compared to examples 2 and 3.
TABLE 7 measurement results of crude protein and crude fat of wormhole
Crude protein (%) Crude fat (%)
Example 1 13.84±0.48 3.97±1.77a
Example 2 13.45±0.67 4.19±1.26a
Example 3 13.83±0.69 8.78±2.22b
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As can be seen from Table 7, the worm sands produced by larval digestion in examples 1, 2 and 3 have no significant difference in crude protein content (P >0.05), and the crude fat content of the worm sands in examples 2 and 3 is significantly higher than that in example 1(P < 0.05).
In summary, example 2 is the feed formulation that is optimized for improving the growth performance of hermetia illucens.
Although the embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the invention and the appended claims, and therefore the scope of the invention is not limited to the embodiments disclosed.

Claims (7)

1. The feed for improving the growth performance of the hermetia illucens is characterized in that: the composition comprises the following components in parts by weight:
0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water.
2. The feed for improving the growth performance of hermetia illucens according to claim 1 or 2, which is characterized in that: the composition comprises the following components in parts by weight:
2.4 parts of soybean oil, 3.6 parts of linseed oil, 0.0012 part of tert-butyl hydroquinone, 78 parts of laying hen manure, 42 parts of water and 2% tween-201.68 parts.
3. The feed for improving the growth performance of hermetia illucens according to claim 1 or 2, which is characterized in that: the linolenic acid content in the linseed oil is high and accounts for more than 50 percent (mass percentage) of the total fatty acid.
4. The feed for improving the growth performance of hermetia illucens according to claim 1 or 2, which is characterized in that: the addition final mass concentration of the linseed oil in the feed is 2%.
5. The method for preparing the feed for improving the growth performance of the hermetia illucens as claimed in any one of claims 1 to 4, wherein the feed comprises the following components: the method comprises the following steps:
s1, adding tert-butyl hydroquinone into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the layer chicken manure to 70% by using water, and then adding 2% of Tween-20;
s3, uniformly mixing the anti-oxidation soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 to obtain feed for improving the growth performance of black soldier flies;
the above operations are all carried out in the environment with the temperature of 18-25 ℃ and the air humidity of less than 25%.
6. The feed for improving the growth performance of hermetia illucens as claimed in any one of claims 1 to 4, for use as a feed for hermetia illucens.
7. The feed for improving the growth performance of hermetia illucens according to any one of claims 1 to 4, wherein the feed is used as a feed for hermetia illucens larvae.
CN202110582846.0A 2021-05-27 2021-05-27 Feed for improving growth performance of hermetia illucens Pending CN113331317A (en)

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CN113940314A (en) * 2021-09-30 2022-01-18 天津农学院 Method for improving composition of hermetia illucens body PUFAs by using linseed oil and detection method thereof
CN115709100A (en) * 2022-10-31 2023-02-24 天津农学院 Preparation and application method of hermetia illucens manure in Fenton-like catalytic material

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CN109757627A (en) * 2019-03-26 2019-05-17 四川十分美环境卫生管理有限公司 A kind of feed and preparation method that can promote the growth of black soldier flies larva

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Publication number Priority date Publication date Assignee Title
CN113940314A (en) * 2021-09-30 2022-01-18 天津农学院 Method for improving composition of hermetia illucens body PUFAs by using linseed oil and detection method thereof
CN113940314B (en) * 2021-09-30 2024-01-09 天津农学院 Method for improving composition of black soldier fly body PUFAs by using linseed oil and detection method thereof
CN115709100A (en) * 2022-10-31 2023-02-24 天津农学院 Preparation and application method of hermetia illucens manure in Fenton-like catalytic material
CN115709100B (en) * 2022-10-31 2023-12-22 天津农学院 Preparation and application method of hermetia illucens manure in Fenton-like catalytic material

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