CN113331317A - Feed for improving growth performance of hermetia illucens - Google Patents
Feed for improving growth performance of hermetia illucens Download PDFInfo
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- CN113331317A CN113331317A CN202110582846.0A CN202110582846A CN113331317A CN 113331317 A CN113331317 A CN 113331317A CN 202110582846 A CN202110582846 A CN 202110582846A CN 113331317 A CN113331317 A CN 113331317A
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- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 70
- 239000000944 linseed oil Substances 0.000 claims abstract description 38
- 235000021388 linseed oil Nutrition 0.000 claims abstract description 38
- 239000003549 soybean oil Substances 0.000 claims abstract description 29
- 235000012424 soybean oil Nutrition 0.000 claims abstract description 29
- 239000010871 livestock manure Substances 0.000 claims abstract description 22
- 210000003608 fece Anatomy 0.000 claims abstract description 21
- 241000287828 Gallus gallus Species 0.000 claims abstract description 17
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000004250 tert-Butylhydroquinone Substances 0.000 claims abstract description 17
- 235000019281 tert-butylhydroquinone Nutrition 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 7
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 7
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 7
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims description 5
- 241001481656 Stratiomyidae Species 0.000 claims description 4
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims description 4
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 4
- 230000001804 emulsifying effect Effects 0.000 claims description 4
- 239000000194 fatty acid Substances 0.000 claims description 4
- 229930195729 fatty acid Natural products 0.000 claims description 4
- 150000004665 fatty acids Chemical class 0.000 claims description 4
- 229960004488 linolenic acid Drugs 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000003064 anti-oxidating effect Effects 0.000 claims description 3
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 4
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 235000019784 crude fat Nutrition 0.000 description 10
- 235000019750 Crude protein Nutrition 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 239000002699 waste material Substances 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 244000144972 livestock Species 0.000 description 5
- 239000003963 antioxidant agent Substances 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 4
- 238000001746 injection moulding Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 241000254173 Coleoptera Species 0.000 description 3
- 241000255925 Diptera Species 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010020112 Hirsutism Diseases 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002216 antistatic agent Substances 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000012084 conversion product Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 235000019261 food antioxidant Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000010806 kitchen waste Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biomedical Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a feed for improving the growth performance of hermetia illucens, which comprises the following components in parts by mass: 0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water. According to the feed for improving the growth performance of the hermetia illucens, the soybean oil, the linseed oil, the tert-butyl hydroquinone and the tween-20 are matched, so that the fat nutrition concentration of the feed is improved, the storage time of the feed for improving the growth performance of the hermetia illucens is prolonged, the oxidation rate of the feed is reduced, and the components of the feed for improving the growth performance of the hermetia illucens are mixed more uniformly. Can effectively prolong the preservation time of the feed for improving the growth performance of the hermetia illucens.
Description
Technical Field
The invention belongs to the technical field of animal feed, relates to feed, and particularly relates to feed for improving the growth performance of hermetia illucens.
Background
Along with the continuous improvement of Chinese economy, the demand of people for meat products is also continuously improved, so that the scale of livestock and poultry breeding production is larger and larger, but the waste treatment and utilization work generated by breeding is relatively lagged. The livestock waste refers to wastes such as livestock manure, livestock house padding, waste feed and scattered hairiness in a breeding farm, and is mostly directly discharged without harmless treatment or dry-wet separation, thereby causing pollution.
The black soldier fly can effectively reduce the accumulation of waste and convert the waste into self nutrient substances of a worm body by recycling the livestock and poultry manure and the kitchen waste, the protein content of larvae of the black soldier fly is high, and the black soldier fly can replace certain protein feed to feed animals, so that the effect of secondary utilization of the waste is achieved, and the economic cost of the feed is also reduced. The black soldier fly is raised simply and easy management, and the required living environment of polypide easily provides, does not need to feed any food in adult raising stage, only need provide drinking water can, only need the amateur time raise in the raising stage of larva can to the food consumption is low during the larva, the food source is extensive, as corrosive insect, various discarded object in the life can both become its food. Meanwhile, the breeding capacity of the insect is high, each time, the egg laying amount is 800-1000, the breeding period is about one month, and the output efficiency and the waste treatment capacity can be greatly improved.
Compared with flies, the hermetia illucens is relatively clean to be cultured, meanwhile, the hermetia illucens cannot enter the human society, and no bacteria or viruses with diseases are carried, researches show that organic matters in excrement can be effectively absorbed by feeding the hermetia illucens by using animal excrement as a raw material, and analysis of research results shows that nitrogen and phosphorus contents in excrement treated by hermetia illucens larvae are obviously reduced, conversion products (insect sand) have no peculiar smell, meanwhile, oviposition behaviors of flies can be effectively prevented, and the number of flies in animal excrement is reduced by more than 90%.
The black soldier fly is used for degrading the livestock and poultry waste, and meanwhile, the black soldier fly is used as the feed, so that the feed is one of the research hotspots in the fields of feed science and environmental protection at present, and the feed nutrition is a key factor for regulating and controlling the growth index and the nutrition index of the black soldier fly. At present, the research results of feed formulas for feeding the hermetia illucens are few, and the feed formulas are difficult to transform, popularize and apply, so that the hermetia illucens feed formula convenient to transform, popularize and apply is found and has important economic value in production practice to improve the growth performance of the hermetia illucens.
Through searching, no patent publication related to the present patent application has been found.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the feed for improving the growth performance of the hermetia illucens.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the feed for improving the growth performance of the hermetia illucens comprises the following components in parts by mass:
0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water.
Further, the composition comprises the following components in parts by weight:
2.4 parts of soybean oil, 3.6 parts of linseed oil, 0.0012 part of tert-butyl hydroquinone, 78 parts of laying hen manure, 42 parts of water and 2% tween-201.68 parts.
Furthermore, the linolenic acid content in the linseed oil is higher and accounts for more than 50% (mass percent) of the total fatty acids.
Further, the linseed oil is added to the feed to a final mass concentration of 2%.
The preparation method of the feed for improving the growth performance of the hermetia illucens comprises the following steps:
s1, adding tert-butyl hydroquinone into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the layer chicken manure to 70% by using water, and then adding 2% of Tween-20;
s3, uniformly mixing the anti-oxidation soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 to obtain feed for improving the growth performance of black soldier flies;
the above operations are all carried out in the environment with the temperature of 18-25 ℃ and the air humidity of less than 25%.
The feed for improving the growth performance of the hermetia illucens is applied to the application of the feed as the feed for the hermetia illucens.
The feed for improving the growth performance of the hermetia illucens is applied to the feed for the hermetia illucens larvae.
The invention has the advantages and positive effects that:
1. according to the feed for improving the growth performance of the hermetia illucens, the soybean oil, the linseed oil, the tert-butyl hydroquinone and the tween-20 are matched, so that the fat nutrition concentration of the feed is improved, the storage time of the feed for improving the growth performance of the hermetia illucens is prolonged, the oxidation rate of the feed is reduced, and the components of the feed for improving the growth performance of the hermetia illucens are mixed more uniformly. Can effectively prolong the preservation time of the feed for improving the growth performance of the hermetia illucens.
2. According to the invention, the black soldier fly is fed by using the feed formula of the linseed oil, wherein the 2% of the linseed oil is added, so that the growth performances of hundred worm weight, worm body length, worm body width, crude fat and the like of the black soldier fly larvae can be obviously improved. The feed formula is suitable for breeding the hermetia illucens larvae, the growth performance of the hermetia illucens is improved, and a foundation is laid for transformation, popularization and application of the hermetia illucens.
3. The feed for improving the growth performance of the hermetia illucens comprises the following components:
soybean oil: soybean oil is an oil extracted by pressing from soybeans, is rich in various valuable nutritional ingredients, and contains a large amount of linoleic acid. In animal production, a certain amount of soybean oil is often added to increase the energy value in the feed. ② linseed oil: the linseed oil is prepared from linseed, the content of unsaturated fatty acid in fatty acid of the linseed oil is higher, and the content of alpha-linolenic acid is as high as more than 50%. In addition, the linseed oil also contains rich nutrient components such as vitamin E, sterol and the like, and researches show that the linseed oil in the feed can improve the animal immunity. ③ tween-20: is a surfactant, is an oil-in-water (O/W) emulsifier, and can be used as solubilizer, dispersing agent, stabilizer, antistatic agent, and lubricant.
Tert-butyl hydroquinone: tert-butyl hydroquinone (tBHQ) is a food antioxidant additive, and researches show that the addition of the tBHQ can prevent the rancidity of oil and fat substances and has an antioxidant effect.
Detailed Description
The following detailed description of the embodiments of the present invention is provided for the purpose of illustration and not limitation, and should not be construed as limiting the scope of the invention.
The raw materials used in the invention are conventional commercial products unless otherwise specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The feed for improving the growth performance of the hermetia illucens comprises the following components in parts by mass:
0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water.
Preferably, the composition comprises the following components in parts by weight:
2.4 parts of soybean oil, 3.6 parts of linseed oil, 0.0012 part of tert-butyl hydroquinone, 78 parts of laying hen manure, 42 parts of water and 2% tween-201.68 parts.
Preferably, the linolenic acid content in the linseed oil is higher and accounts for more than 50% (mass percent) of the total fatty acids.
Preferably, the linseed oil is added to the feed to a final mass concentration of 2%.
The preparation method of the feed for improving the growth performance of the hermetia illucens comprises the following steps:
s1, adding tert-butyl hydroquinone into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the layer chicken manure to 70% by using water, and then adding 2% of Tween-20;
s3, uniformly mixing the anti-oxidation soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 to obtain feed for improving the growth performance of black soldier flies;
the above operations are all carried out in the environment with the temperature of 18-25 ℃ and the air humidity of less than 25%.
The feed for improving the growth performance of the hermetia illucens is applied to the application of the feed as the feed for the hermetia illucens.
The feed for improving the growth performance of the hermetia illucens is applied to the feed for the hermetia illucens larvae.
Specifically, the preparation and detection examples are as follows:
examples 1-3 feed for improving growth performance of hermetia illucens
Examples 1 to 3 are feeds for improving the growth performance of black soldier flies, and the composition of raw materials for preparing antioxidant soybean oil linseed oil is shown in table 1, and the composition of raw materials for preparing chicken manure is shown in table 2.
Table 1 examples 1-3 raw materials for antioxidant soybean oil, linseed oil
TABLE 2 examples 1-3 preparation of raw materials for chicken manure
Examples 1-3 methods for preparing feed for improving the growth performance of hermetia illucens are performed according to the following steps, all of which are performed in an environment with a temperature of 18-25 ℃ and an air humidity of less than 25%, as follows:
s1, adding tert-butyl hydroquinone (200 mu g/g grease) into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the chicken manure to 70% by using water, and adding 2% of Tween-20 which accounts for 2% of the water content of the adjusted chicken manure;
s3, mixing the antioxidant soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 uniformly to obtain the feed for improving the growth performance of the hermetia illucens.
The correlation test of the present invention is as follows:
the verification test of the feed for improving the growth performance of the hermetia illucens comprises the following steps:
(1) test animal selection and grouping
By adopting a complete random design of a single-factor experimental scheme, 2400 black soldier fly larvae with similar hundred-worm weight and strong peristalsis capacity and 1 year old are selected and randomly divided into 3 groups (example 1, example 2 and example 3), each group is divided into 4 replicates, each replicate 200 larvae, fresh chicken manure added with 5% of soybean oil is taken as a control group (example 1), and fresh chicken manure added with linseed oil instead of the soybean oil is taken as a test group respectively (example 2) and 5% (example 3).
(2) Feed and feeding management
The control group was fed with the feed of example 1, and the test group was fed with the test feed supplemented with linseed oil instead of soybean oil (example 2) and 5% (example 3) of the feed for improving the growth performance of hermetia illucens, which were fed freely during the test. A650 ml disposable injection molding meal box was used as a feeding container for hermetia illucens larvae, and a 12cm x 6cm vent was cut out with scissors in the middle of the meal box cover to facilitate the breathing of the hermetia illucens larvae. Adding 1-year larvae and feed for improving the growth performance of hermetia illucens into a disposable injection-molded lunch box, putting the meal box into a biochemical incubator, keeping the temperature at 28 ℃, ventilating and replenishing water every day, wherein the water replenishing amount is based on the aim of replenishing the humidity of the feed to about 70%, the feed is added for multiple times, and the rest 50% of the feed is added as reference.
(3) Collection and index determination
The first blackened larva appearing in the disposable injection molding lunch box is used as a signal, the disposable injection molding lunch box is collected completely at one time, and residual excrement on the surface of the disposable injection molding lunch box is cleaned up by utilizing the larva wriggling characteristic. Starvation treatment is carried out for 24 hours, so that undigested residues in the digestive tract of the polypide are discharged. And then removing initial water in an electrothermal blowing dry box at the temperature of 60-70 ℃ to prepare a semi-dry sample, and measuring the weight of the beetles, the length of the beetles, the width of the beetles, crude protein and crude fat according to the following method.
Measuring the weight of the hundred insects: taking 100 6-th-instar hermetia illucens larvae by a quartering method in each group, and weighing by using an analytical balance;
measuring the length and width of the worm body: taking 50 6 th larvae of hermetia illucens in each group by a quartering method, and measuring the length (the length from head to tail) and the width (the widest distance on two sides of the larvae) of the larvae by using vernier calipers;
measuring the crude protein of the polypide (Kjeldahl method): grinding the air-dried worm bodies into powder by using a mortar, accurately weighing about 0.5g (accurate to 0.1mg) of worm powder into a digestion tube, adding 10ml of concentrated sulfuric acid, digesting for 1h at 420 ℃, and performing full-automatic crude protein analysis by using a SCINOKT260 Kjeldahl azotometer;
fourthly, measuring the crude fat of the worm body (Soxhlet extraction method): the air-dried worm bodies were ground into powder using a mortar, and about 2g (to an accuracy of 0.1mg) of the worm powder was weighed accurately into a filter paper cartridge, and the mass was recorded as m. Weighing the mass of the empty aluminum cup, and recording as m1Placing the filter paper cylinder and the aluminum cup into a Soxtec 8000 Soxhlet extractor for extraction, and weighing the mass of the aluminum cup after extraction is finished, and recording the mass as m2. The worm dry matter has a crude fat content of (m)2-m1)/m。
(4) Test results
TABLE 4 Pest determination results
Initial weight/g | Total weight of fresh insect/g | Weight of hundred insects/g | |
Example 1 | 0.51±0.00 | 21.88±1.13ab | 11.99±0.68ab |
Example 2 | 0.51±0.01 | 23.00±1.32a | 12.10±0.52a |
Example 3 | 0.50±0.01 | 19.83±1.06b | 11.17±0.37b |
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As can be seen from Table 4, there is no significant difference in initial insect weights in examples 1, 2 and 3, and test comparability is ensured.
Example 2 significantly increased the total weight and hundred weight of hermetia illucens (P <0.05) compared to example 3.
TABLE 5 measurement results of length and width of worm
Length/cm of insect body | Width/cm of insect body | |
Example 1 | 1.709±0.115a | 0.497±0.037b |
Example 2 | 1.722±0.106a | 0.514±0.039a |
Example 3 | 1.640±0.136b | 0.507±0.040a |
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As shown in Table 5, the length of the black soldier fly larvae was significantly increased (P <0.05) in examples 1 and 2 as compared with example 3, and the width of the black soldier fly larvae was significantly increased (P <0.05) in example 2 as compared with example 1
TABLE 6 measurement results of crude protein and crude fat of polypide
Crude protein (%) | Crude fat (%) | |
Example 1 | 48.72±2.38a | 25.50±2.50b |
Example 2 | 45.99±1.20b | 27.53±2.12ab |
Example 3 | 43.31±1.25b | 31.34±2.76a |
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As shown in table 6, in examples 2 and 3, the crude fat content of the black soldier fly larvae was significantly increased (P <0.05) as compared to example 1; in example 1, the crude protein content was significantly increased (P <0.05) compared to examples 2 and 3.
TABLE 7 measurement results of crude protein and crude fat of wormhole
Crude protein (%) | Crude fat (%) | |
Example 1 | 13.84±0.48 | 3.97±1.77a |
Example 2 | 13.45±0.67 | 4.19±1.26a |
Example 3 | 13.83±0.69 | 8.78±2.22b |
Note: in the table, the difference of the shoulder mark letters indicates that the difference is obvious (P <0.05), the letters are the same or no letters indicate that the difference is not obvious (P >0.05)
As can be seen from Table 7, the worm sands produced by larval digestion in examples 1, 2 and 3 have no significant difference in crude protein content (P >0.05), and the crude fat content of the worm sands in examples 2 and 3 is significantly higher than that in example 1(P < 0.05).
In summary, example 2 is the feed formulation that is optimized for improving the growth performance of hermetia illucens.
Although the embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the invention and the appended claims, and therefore the scope of the invention is not limited to the embodiments disclosed.
Claims (7)
1. The feed for improving the growth performance of the hermetia illucens is characterized in that: the composition comprises the following components in parts by weight:
0-5.99 parts of soybean oil, 0.01-6 parts of linseed oil, 78 parts of egg chicken manure, 0.0012 part of tert-butyl hydroquinone, 2% tween-201.68 parts and 42 parts of water.
2. The feed for improving the growth performance of hermetia illucens according to claim 1 or 2, which is characterized in that: the composition comprises the following components in parts by weight:
2.4 parts of soybean oil, 3.6 parts of linseed oil, 0.0012 part of tert-butyl hydroquinone, 78 parts of laying hen manure, 42 parts of water and 2% tween-201.68 parts.
3. The feed for improving the growth performance of hermetia illucens according to claim 1 or 2, which is characterized in that: the linolenic acid content in the linseed oil is high and accounts for more than 50 percent (mass percentage) of the total fatty acid.
4. The feed for improving the growth performance of hermetia illucens according to claim 1 or 2, which is characterized in that: the addition final mass concentration of the linseed oil in the feed is 2%.
5. The method for preparing the feed for improving the growth performance of the hermetia illucens as claimed in any one of claims 1 to 4, wherein the feed comprises the following components: the method comprises the following steps:
s1, adding tert-butyl hydroquinone into the added soybean oil and linseed oil by using a seed method to prevent the soybean oil and the linseed oil from being oxidized;
s2, adjusting the humidity of the layer chicken manure to 70% by using water, and then adding 2% of Tween-20;
s3, uniformly mixing the anti-oxidation soybean oil linseed oil prepared in the step S1 and the prepared chicken manure with emulsifying capacity prepared in the step S2 to obtain feed for improving the growth performance of black soldier flies;
the above operations are all carried out in the environment with the temperature of 18-25 ℃ and the air humidity of less than 25%.
6. The feed for improving the growth performance of hermetia illucens as claimed in any one of claims 1 to 4, for use as a feed for hermetia illucens.
7. The feed for improving the growth performance of hermetia illucens according to any one of claims 1 to 4, wherein the feed is used as a feed for hermetia illucens larvae.
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CN113940314A (en) * | 2021-09-30 | 2022-01-18 | 天津农学院 | Method for improving composition of hermetia illucens body PUFAs by using linseed oil and detection method thereof |
CN115709100A (en) * | 2022-10-31 | 2023-02-24 | 天津农学院 | Preparation and application method of hermetia illucens manure in Fenton-like catalytic material |
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Cited By (4)
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CN113940314B (en) * | 2021-09-30 | 2024-01-09 | 天津农学院 | Method for improving composition of black soldier fly body PUFAs by using linseed oil and detection method thereof |
CN115709100A (en) * | 2022-10-31 | 2023-02-24 | 天津农学院 | Preparation and application method of hermetia illucens manure in Fenton-like catalytic material |
CN115709100B (en) * | 2022-10-31 | 2023-12-22 | 天津农学院 | Preparation and application method of hermetia illucens manure in Fenton-like catalytic material |
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