CN113330106A - 用于在自动化生物反应器中使用的细胞分离 - Google Patents

用于在自动化生物反应器中使用的细胞分离 Download PDF

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Publication number
CN113330106A
CN113330106A CN201980089612.3A CN201980089612A CN113330106A CN 113330106 A CN113330106 A CN 113330106A CN 201980089612 A CN201980089612 A CN 201980089612A CN 113330106 A CN113330106 A CN 113330106A
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cell
cartridge
separation filter
automated
chamber
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Pending
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CN201980089612.3A
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English (en)
Chinese (zh)
Inventor
E·麦卡菲
Y·施
S·班达帕里
E·亚伯拉罕
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Lonza Walkersville Inc
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Lonza Walkersville Inc
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Publication of CN113330106A publication Critical patent/CN113330106A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Electromagnetism (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980089612.3A 2018-12-11 2019-12-11 用于在自动化生物反应器中使用的细胞分离 Pending CN113330106A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862778078P 2018-12-11 2018-12-11
US62/778,078 2018-12-11
PCT/US2019/065731 WO2020123655A1 (en) 2018-12-11 2019-12-11 Cell isolation for use in automated bioreactors

Publications (1)

Publication Number Publication Date
CN113330106A true CN113330106A (zh) 2021-08-31

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980089612.3A Pending CN113330106A (zh) 2018-12-11 2019-12-11 用于在自动化生物反应器中使用的细胞分离

Country Status (9)

Country Link
US (2) US20200181562A1 (enExample)
EP (1) EP3894540A4 (enExample)
JP (1) JP2022511935A (enExample)
KR (1) KR20210102928A (enExample)
CN (1) CN113330106A (enExample)
CA (1) CA3122288A1 (enExample)
IL (1) IL283819A (enExample)
SG (1) SG11202106030QA (enExample)
WO (1) WO2020123655A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6987080B2 (ja) 2016-05-05 2021-12-22 サウスウェスト リサーチ インスティテュート 細胞増殖のための三次元バイオリアクター及び関連用途
US11149244B2 (en) 2018-04-04 2021-10-19 Southwest Research Institute Three-dimensional bioreactor for T-cell activation and expansion for immunotherapy
JP7376576B2 (ja) 2018-09-24 2023-11-08 サウスウェスト リサーチ インスティテュート 三次元バイオリアクター
WO2021206686A1 (en) * 2020-04-07 2021-10-14 Hewlett-Packard Development Company, L.P. Microfluidic chip cell sorting and transfection
WO2022060963A1 (en) * 2020-09-17 2022-03-24 Lonza Sales Ag Automated downstream processing of a biologic product
AU2022377625B2 (en) 2021-10-28 2025-01-02 Southwest Research Institute Devices for cell separation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130143195A1 (en) * 2011-12-05 2013-06-06 Pall Corporation Leukocyte purification
US20170096627A1 (en) * 2004-05-26 2017-04-06 Octane Biotech, Inc. Advanced tissue engineering system

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763266A (en) * 1989-06-15 1998-06-09 The Regents Of The University Of Michigan Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells
CA2159005C (en) * 1993-03-23 2007-06-19 Yves Claude Nicolau Method and apparatus for encapsulation of biologically-active substances in cells
US5688687A (en) * 1995-06-07 1997-11-18 Aastrom Biosciences, Inc. Bioreactor for mammalian cell growth and maintenance
US5989913A (en) * 1998-07-02 1999-11-23 Charles Daniel Anderson Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using the same
US20020146817A1 (en) * 2000-10-02 2002-10-10 Cannon Thomas F. Automated bioculture and bioculture experiments system
US8986944B2 (en) * 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
WO2003087292A2 (en) * 2002-04-08 2003-10-23 Millenium Biologix Inc. Automated tissue engineering system
GB201319139D0 (en) * 2013-10-30 2013-12-11 Exmoor Pharma Concepts Ltd Apparatus and method for filtration of a suspension
JP6619429B2 (ja) * 2014-12-03 2019-12-11 ベンド リサーチ， インコーポレイテッド 使い捨て可能な細胞除去システム
WO2017109071A1 (en) * 2015-12-22 2017-06-29 Ge Healthcare Bio-Sciences Corp. Improvements in and relating to cell harvesting apparatus
GB201617713D0 (en) * 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
JP2020530979A (ja) * 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170096627A1 (en) * 2004-05-26 2017-04-06 Octane Biotech, Inc. Advanced tissue engineering system
US20130143195A1 (en) * 2011-12-05 2013-06-06 Pall Corporation Leukocyte purification

Also Published As

Publication number Publication date
EP3894540A1 (en) 2021-10-20
US20200181562A1 (en) 2020-06-11
SG11202106030QA (en) 2021-07-29
WO2020123655A1 (en) 2020-06-18
EP3894540A4 (en) 2022-11-16
IL283819A (en) 2021-07-29
JP2022511935A (ja) 2022-02-01
KR20210102928A (ko) 2021-08-20
CA3122288A1 (en) 2020-06-18
US20220267712A1 (en) 2022-08-25

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