CN113288950B

CN113288950B - Traditional Chinese medicine composition for losing weight and reducing blood sugar and preparation thereof

Info

Publication number: CN113288950B
Application number: CN202110695744.XA
Authority: CN
Inventors: 刘向前; 郑贤珠
Original assignee: Changsha Broad Ocean Bio Science And Technique Co ltd
Current assignee: Changsha Broad Ocean Bio Science And Technique Co ltd
Priority date: 2021-06-23
Filing date: 2021-06-23
Publication date: 2022-05-27
Anticipated expiration: 2041-06-23
Also published as: GB2608507A; CN113288950A; GB202208875D0

Abstract

The invention discloses a traditional Chinese medicine composition for losing weight and reducing blood sugar and a preparation thereof. The weight-losing and blood sugar-reducing traditional Chinese medicine composition comprises the following raw materials in parts by weight: 25-30 parts of dried orange peel, 8-12 parts of cassia seed, 10-13 parts of houttuynia cordata, 19-24 parts of exocarpium benincasae, 9-12 parts of lotus pedicel, 10-12 parts of acanthopanax senticosus and 10-15 parts of phaseolus calcaratus. The invention adopts water solutions with different pH values to extract effective components in the traditional Chinese medicinal materials, and the extracted effective components are applied to preparing the weight-losing and blood-sugar-reducing traditional Chinese medicine preparation. The weight-losing and blood-sugar-reducing traditional Chinese medicine composition disclosed by the invention not only has the effects of losing weight and reducing blood sugar, but also can protect blood vessels.

Description

Traditional Chinese medicine composition for losing weight and reducing blood sugar and preparation thereof

Technical Field

The invention relates to the technical field of weight reduction of traditional Chinese medicines, in particular to a weight-reducing and sugar-reducing traditional Chinese medicine composition and a preparation thereof.

Background

With changes in dietary structure and lifestyle, obesity and type 2 diabetes have become epidemic diseases worldwide. High-fat foods such as pizza, hamburgers, high-fat meat products, chocolate, fried foods, candies, cakes, milk tea, biscuits and the like are increasingly favored by consumers, and the high-fat foods are easy to cause obesity after being eaten for a long time, so that various chronic diseases are further induced. With the change of life style of people, the faster and faster life rhythm causes that many people do not have time to exercise for weight reduction, so compared with exercise for weight reduction, the drug weight reduction audience is wider and wider.

At present, a great variety of weight-reducing medicines are available on the market, but the only medicine approved by the state for weight reduction is orlistat, which belongs to the inhibitor of gastrointestinal lipase, and through combining with gastric lipase and pancreatic lipase in the stomach and small intestine, the enzyme is inactivated, and the enzyme after inactivation can not decompose fat in food into absorbable nutrient substances, and the fat which can not be digested can be discharged out of the body through the gastrointestinal tract, so that the calorie intake is reduced, and the weight-reducing medicine has a better effect on abdominal obesity of men. However, the medicine has certain side effects, mainly including oil discharge, stool uncontrolled, and gastrointestinal side effects such as nausea and the like of some patients.

Compared with western medicines, the traditional Chinese medicine is safer in weight reduction, has smaller side effects, and most of the current traditional Chinese medicine weight reduction products are tea-type weight reduction products, but the tea-type weight reduction products have smaller effect and less obvious effect, so that the development of a traditional Chinese medicine composition with an obvious weight reduction effect is a technical problem to be solved urgently in the field.

Disclosure of Invention

The invention aims to provide a weight-losing and blood sugar-reducing traditional Chinese medicine composition and a preparation thereof.

In order to achieve the purpose, the invention provides the following technical scheme:

one of the technical schemes of the invention is as follows: the weight-losing and blood sugar-reducing traditional Chinese medicine composition is provided, and comprises the following raw materials in parts by weight: 25-30 parts of dried orange peel, 8-12 parts of cassia seed, 10-13 parts of houttuynia cordata, 19-24 parts of exocarpium benincasae, 9-12 parts of lotus pedicel, 10-12 parts of acanthopanax senticosus and 10-15 parts of phaseolus calcaratus.

Preferably, the raw materials comprise the following components in parts by weight: 25 parts of dried orange peel, 10 parts of cassia seed, 10 parts of heartleaf houttuynia herb, 20 parts of Chinese waxgourd peel, 10 parts of lotus pedicel, 10 parts of acanthopanax root and 15 parts of phaseolus calcaratus.

Dried orange peel: warm in nature, pungent and bitter in flavor. Regulate qi-flowing for strengthening spleen, regulate middle energizer, dry dampness and resolve phlegm. Can be used for treating abdominal distention, pain, and dyspepsia due to qi stagnation of spleen and stomach. Chest oppression, abdominal distension, poor appetite and loose stool due to damp-turbidity obstruction. Cough and dyspnea due to phlegm-dampness obstructing the lung. Warm can move qi, pungent can disperse, bitter can drain water.

Cassia seed: sweet and bitter in flavor, cold in nature and slightly salty. Clear liver and improve vision, moisten intestines and relax bowels. Can be used for treating conjunctival congestion, photophobia, lacrimation, headache, vertigo, dim eyesight, and constipation.

Houttuynia cordata: pungent and slightly cold. Clearing away heat and toxic material, curing abscess and discharging pus, inducing diuresis and treating stranguria. Can be used for treating lung abscess with purulent vomiting, phlegm heat, cough and asthma, dysentery, pyretic stranguria, carbuncle, swelling, and sore.

White gourd peel: sweet taste and cool nature. Induce diuresis to alleviate edema. Can be used for treating edema, dysuresia, summer heat, thirst, and scanty and brownish urine.

Lotus pedicel: bitter and mild in nature and taste. Clearing away summer heat, removing dampness, stopping bleeding, preventing miscarriage, invigorating middle warmer, and replenishing qi.

Radix acanthopanacis senticosi: slightly bitter and pungent in flavor. Replenishing qi to invigorate the spleen, tonifying the kidney and soothing the nerves.

Small red beans: sweet and sour in flavor and neutral in nature. Induce diuresis to alleviate edema, remove toxicity and expel pus. Can be used for treating edema, swelling, beriberi, edema, jaundice, dark urine, rheumatism, pyretic arthralgia, carbuncle, swelling, sore, and abdominal pain due to intestinal abscess.

The second technical scheme of the invention is as follows: provides a Chinese medicinal preparation for reducing weight and blood sugar, which takes the extract of the Chinese medicinal composition for reducing weight and blood sugar as an effective component.

Preferably, the dosage form of the weight-losing and blood-sugar-reducing traditional Chinese medicine preparation is any one of oral liquid, granules, tablets or capsules.

Preferably, the extraction method of the extract comprises the following steps: crushing and sieving the raw materials, extracting the raw materials by using an aqueous solution with the pH of 1.8-2.0, and filtering to obtain filter residue a; extracting the filter residue a with an aqueous solution with the pH value of 6.0-6.5, and filtering to obtain filter residue b; extracting the filter residue b by using an aqueous solution with the pH value of 8.5-9.0, and filtering; mixing the filtrates, and removing solvent from the supernatant to obtain extract.

Preferably, the sieving is 20-40 mesh sieving.

Preferably, hydrochloric acid is used for adjusting the pH value of the aqueous solution with the pH value of 1.8-2.0 and the aqueous solution with the pH value of 6.0-6.5; and adjusting the pH value of the water solution with the pH value of 8.5-9.0 by using sodium bicarbonate.

Preferably, when the extract is extracted by using an aqueous solution with the pH of 1.8-2.0, the extraction time is 120-140 min, and the dosage of the aqueous solution is 10 times of the dry weight of the traditional Chinese medicinal materials; when the extract is extracted by using an aqueous solution with the pH of 6.0-6.5, the extraction time is 60-70 min, and the dosage of the aqueous solution is 8 times of the dry weight of the traditional Chinese medicinal materials; when the traditional Chinese medicine is extracted by using an aqueous solution with the pH of 8.5-9.0, the extraction time is 30-35 min, and the dosage of the aqueous solution is 8 times of the dry weight of the traditional Chinese medicine.

The invention has the following beneficial technical effects:

the weight-losing and blood sugar-reducing traditional Chinese medicine composition can play a role in controlling weight by inhibiting the absorption of fat in food.

The weight-losing and blood sugar-reducing traditional Chinese medicine composition also has the effects of reducing blood sugar and protecting blood vessels.

Drawings

Fig. 1 is a graph of three groups of mice fed in vivo for 12 weeks, wherein a is a normal diet group, b is a high fat diet group, and c is a drug group;

FIG. 2 is the average body weight of three groups of mice in an in vivo experiment at 0-12 weeks;

FIG. 3 is the average food intake of three groups of mice in 1-12 weeks in vivo;

FIG. 4 shows the food intake and weight gain of three mice in vivo, wherein a is the weight gain of three mice and b is the ratio of food efficiency of three mice;

FIG. 5 shows the fat content in the dry feces of mice in the high fat diet group and the drug group collected at

weeks

2, 5, and 10 in the in vivo experiment;

FIG. 6 shows the serum total cholesterol levels of three groups of mice in vivo;

FIG. 7 shows the content of triglycerides in the serum of three groups of mice in an in vivo experiment;

FIG. 8 shows the serum HDL and LDL/VLDL of three groups of mice in vivo experiments, wherein a is the serum HDL of three groups of mice, and b is the serum LDL/VLDL of three groups of mice;

FIG. 9 shows the liver tissue weights of three groups of mice in an in vivo experiment;

FIG. 10 shows the white adipose tissue weights of three groups of mice in an in vivo experiment;

FIG. 11 shows the weights of kidney tissues of three groups of mice in an in vivo experiment;

FIG. 12 shows spleen tissue weights of three groups of mice in an in vivo experiment;

FIG. 13 is a photograph of liver tissues of one mouse selected from three groups of mice in an in vivo experiment;

FIG. 14 is a photograph of liver tissue sections of one mouse selected from three groups of mice in an in vivo experiment;

FIG. 15 is a white adipose tissue section of one mouse selected from each of three groups of mice in an in vivo experiment;

FIG. 16 is a photograph of a kidney tissue section of one mouse selected from each of three groups of mice in an in vivo experiment;

FIG. 17 is a photograph of a spleen tissue section of one mouse selected from each of three groups of mice in an in vivo experiment.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but rather as a more detailed description of certain aspects, features and embodiments of the invention. It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

In addition, for numerical ranges in the present disclosure, it is understood that each intervening value, to the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

Example 1

Drying pericarpium citri reticulatae, semen cassiae, houttuynia cordata, exocarpium benincasae, lotus pedicel, acanthopanax senticosus and phaseolus calcaratus in an oven at 105 ℃ for 4 hours, drying, respectively crushing, and sieving by a sieve of 20-40 meshes; weighing sieved powder according to the weight ratio of 2.5kg of dried orange peel, 1kg of cassia seed, 1kg of houttuynia cordata, 2kg of exocarpium benincasae, 1kg of lotus pedicel, 1kg of acanthopanax senticosus and 1.5kg of phaseolus calcaratus, and adding the sieved powder into an extraction tank; adding 10 times of water solution (pH value adjusted with hydrochloric acid) with pH of 2.0, heating and refluxing for 140min, and filtering to obtain extractive solution; heating and refluxing the residue with 8 times of water solution (pH adjusted with hydrochloric acid) with pH of 6.0 for 70min, and filtering to obtain extractive solution; heating and refluxing the residue with 8 times of water solution (pH adjusted by sodium bicarbonate) with pH of 8.5 (by weight of medicinal materials) for 35min, and filtering to obtain extractive solution; mixing the filtrates, centrifuging at 2000rpm for 10min, concentrating the supernatant under reduced pressure to obtain extract, and freeze drying to obtain 1.48kg extract with extraction rate of 14.8%.

Example 2

Drying pericarpium citri reticulatae, semen cassiae, houttuynia cordata, exocarpium benincasae, lotus pedicel, acanthopanax senticosus and phaseolus calcaratus in an oven at 105 ℃ for 4 hours, drying, respectively crushing, and sieving by a sieve of 20-40 meshes; weighing and sieving powder according to the weight ratio of 3kg of dried orange peel, 0.8kg of cassia seed, 1.3kg of houttuynia cordata, 1.9kg of exocarpium benincasae, 1.2kg of lotus pedicel, 1kg of acanthopanax senticosus and 1.3kg of phaseolus calcaratus, and adding the powder into an extraction tank; adding 10 times of water solution (pH value adjusted with hydrochloric acid) with pH of 1.8, heating and refluxing for 140min, and filtering to obtain extractive solution; heating and refluxing the residue with 8 times of water solution (pH adjusted with hydrochloric acid) with pH of 6.0 for 60min, and filtering to obtain extractive solution; heating and refluxing the residue with 8 times of water solution (pH adjusted by sodium bicarbonate) with pH of 9.0 for 30min, and filtering to obtain extractive solution; mixing the filtrates, centrifuging at 1800rpm for 13min, concentrating the supernatant under reduced pressure to obtain extract, and freeze drying to obtain 1.55kg extract with extraction rate of 14.62%.

Example 3

Drying pericarpium citri reticulatae, semen cassiae, houttuynia cordata, exocarpium benincasae, lotus calyx, acanthopanax senticosus and phaseolus calcaratus in an oven at 105 ℃ for 4 hours, respectively crushing after drying, and sieving by a sieve of 20-40 meshes; weighing sieved powder according to the weight ratio of 2.8kg of dried orange peel, 1.2kg of cassia seed, 1.1kg of houttuynia cordata, 2.4kg of exocarpium benincasae, 0.9kg of lotus pedicel, 1.2kg of acanthopanax root and 1kg of phaseolus calcaratus, and adding the sieved powder into an extraction tank; adding 10 times of water solution (pH value adjusted with hydrochloric acid) with pH of 2.0, heating and refluxing for 120min, and filtering to obtain extractive solution; heating and refluxing the residue with 8 times of water solution (pH adjusted with hydrochloric acid) with pH of 6.5 for 70min, and filtering to obtain extractive solution; heating and refluxing the residue with 8 times of water solution (pH adjusted by sodium bicarbonate) with pH of 8.5 (by weight of medicinal materials) for 35min, and filtering to obtain extractive solution; mixing the filtrates, centrifuging at 1500rpm for 15min, concentrating the supernatant under reduced pressure to obtain extract, and freeze drying to obtain 1.59kg extract with extraction rate of 15.0%.

In vitro experiments

The extract obtained in example 1 was formulated into solutions of various concentrations, and the antioxidant activity and anti-adipogenic activity were measured.

(1) Antioxidant activity: the DPPH radical scavenging activity (results shown in Table 1), ABTS radical scavenging activity (results shown in Table 2) and the half inhibitory concentrations of DPPH radicals and ABTS radicals (results shown in Table 3) were measured separately for solutions of different concentrations of the extract obtained in example 1.

TABLE 1 Activity for scavenging DPPH free radicals

Concentration (μ g/mL)	DPPH radical scavenging ratio (%)
		1250	98.83
625	90.86
		312.5	57.83
156.25	34.17
		78.125	18.70
39.0625	10.27
		19.5313	5.35
9.7656	3.01
		4.8828	1.13
2.4414	-0.74
		1.2207	0.20

TABLE 2 Activity for scavenging ABTS free radicals

TABLE 3 semi-inhibitory concentrations of DPPH and ABTS radicals

As can be seen from tables 1 to 3, the higher the clearance of DPPH free radicals and ABTS free radicals with the increase of the concentration of the extract in example 1, the traditional Chinese medicine composition of the present invention has antioxidant activity.

In vivo experiments

The 16C 57BL/6 mice were divided into three groups, normal diet group (5 mice), high fat diet group (6 mice) and drug group (5 mice), the normal diet group mice were fed with the food of the normal diet formula in table 4, the high fat diet group and drug group mice were fed with the food of the high fat diet formula in table 4 (the nutrient components of the normal diet formula and the high fat diet formula are shown in table 5), the food and water were freely available to each group of mice, wherein the drug group was subjected to gastric gavage twice a day, the drug was the extract obtained in example 1, each dose was 100mg/kg, and the three groups of mice were fed for a total of 12 weeks.

TABLE 4 ingredients of the experimental diets

TABLE 5

Nutrient composition	Normal diet formula	High fat diet formula
			Protein,% g (w/w) (% kcal)	18.0(21.03)	26.2(20.0)
Carbohydrate,% g (w/w) (% kcal)	62.7(65.30)	26.3(20.0)
			Fat,% g (w/w) (% kcal)	5.2(13.67)	34.9(60.0)
Physiological energy value of food, kcal/g	3.43	5.21

(1) The pictures after feeding three groups of mice for 12 weeks are shown in fig. 1, in which a is a normal diet group, b is a high fat diet group, and c is a drug group (one mouse was excluded from data analysis due to abnormal weight gain). As can be seen from FIG. 1, the mice in the high-fat diet group are significantly fatter than the mice in the normal diet group and the drug group, which shows that the weight-losing and blood-glucose-lowering traditional Chinese medicine composition can play a role in controlling weight.

(2) The body weight of each group of mice was measured weekly during the experiment and the results are shown in figure 2 (statistical significance was calculated using one-way anova and Dunnett's multiple comparison test with high fat diet group as control group; P <0.05 in the figure; P < 0.01; P < 0.001). As can be seen from fig. 2, the mice on the high-fat diet group had the fastest normal body weight, the mice on the drug group had the slowest normal diet group, and the weight-losing and blood-glucose-lowering traditional Chinese medicine composition provided by the invention has the function of controlling body weight.

(3) During the experiment, average food intake per week was recorded for three groups of mice, and the results are shown in fig. 3. As can be seen from fig. 3, the food intake was significantly smaller in the high fat diet group and the drug group than in the normal diet group.

(4) The food efficacy ratio of each group was calculated based on the food intake and weight gain of three groups of mice, and the results are shown in fig. 4, where a is the weight gain of three groups of mice, and b is the food efficacy ratio of three groups of mice (statistical significance was calculated using one-way anova and Dunnett's multiple comparison test with high fat diet group as control group; P < 0.05; P < 0.01; P < 0.001). As can be seen from fig. 4, the weight gain of the mice in the drug group was slower and the food efficacy ratio was relatively lower than that in the high fat diet group, indicating that the Chinese medicinal composition of the present invention can play a role in reducing food intake and reducing body weight.

(5) Collecting the feces of mice in a high-fat diet group and a medicine group in the 2 nd, 5 th and 10 th weeks of the experiment, taking 0.5-1 g of dry feces, adding 3ml of water for moisturizing, and adopting methanol: the fat content in the dry feces of the mice was calculated by extracting the feces fat with a chloroform (1:2) mixed solution, and the results are shown in FIG. 5. As can be seen from fig. 5, in

weeks

2 and 5, the fat content in the dry feces of the mice in the high-fat diet group and the drug group is not greatly different, and in week 10, the fat content in the dry feces of the mice in the drug group is obviously higher than that in the high-fat diet group, which indicates that the fat absorption of the mice can be inhibited by taking the weight-reducing and sugar-reducing traditional Chinese medicine composition.

(6) Blood glucose levels were measured in three groups of mice at

weeks

0, 3, 6, 9, and 12 of the experiment and the results are shown in table 6.

TABLE 6

Remarking: statistical significance was calculated in table 8 using one-way analysis of variance and Dunnett's multiple comparison test with the high fat diet group as the control group; wherein denotes P < 0.05; denotes P < 0.001.

As can be seen from Table 6, as the experiment proceeded, the blood sugar level of the mice in the drug group was gradually lower from the beginning to be close to that of the mice in the high fat diet group than that of the mice in the high fat diet group, which indicates that the weight-reducing and blood sugar-lowering Chinese medicinal composition of the present invention can lower the blood sugar level of the mice.

(7) At the end of the experiment, serum insulin and homeostatic model assessment (HOMA) -Insulin Resistance (IR) of three groups of mice were determined and the results are shown in table 7.

TABLE 7

Remarking: statistical significance was calculated in table 9 using one-way analysis of variance and Dunnett's multiple comparison test with the high fat diet group as the control group; wherein denotes P < 0.05; denotes P < 0.01; denotes P < 0.001.

As can be seen from table 7, compared with the group with high-fat diet, the group with the drug can significantly reduce the content of serum insulin and fasting plasma glucose, which indicates that the Chinese medicinal composition has significant hypoglycemic effect.

(8) After the experiment, the contents of total cholesterol and triglyceride in the sera of three groups of mice are determined, and the three groups are subjected to a blood lipid profile analysis. FIG. 6 shows the total cholesterol levels in the sera of three groups of mice; FIG. 7 is the triglyceride levels in the sera of three groups of mice; FIG. 8 shows HDL and LDL/VLDL in the sera of three groups of mice, where a is HDL in the sera of three groups of mice and b is LDL/VLDL in the sera of three groups of mice (statistical significance was calculated using one-way anova and Dunnett's multiple comparison test with the group of high fat diets; P < 0.05; P < 0.01; P < 0.001). As can be seen from fig. 6 to 8, the treated group significantly reduced the total cholesterol and triglyceride levels in serum, increased the high-density lipoprotein level in serum, and reduced LDL/VLDL, as compared to the high-fat diet group. The medicine group has the function of promoting lipid metabolism, thereby playing the role of reducing lipid.

(9) After the experiment is finished, the contents of serum total cholesterol and serum high-density lipoprotein of three groups of mice are measured, and the atherosclerosis index and the protection rate of the weight-losing and blood-sugar-reducing traditional Chinese medicine composition are calculated, and the result is shown in table 8.

TABLE 8

Group of	Atherosclerosis Index (AI)	Protective Rate (%)
			Group of normal diet	0.92±0.50	-
High fat diet group	1.37±0.21	-
			Drug group	0.84±0.17	38.71

The calculation formula of the Atherosclerosis Index (AI) in Table 8 is as follows:

wherein TC is serum total cholesterol content, HDL is serum high density lipoprotein content;

the formula for calculating the protection rate (%) is as follows:

as can be seen from Table 8, compared with the group with high-fat diet, the group with the medicine can significantly reduce the atherosclerosis index, and the protection rate can reach 38.71%, which shows that the traditional Chinese medicine composition has the effect of reducing blood fat.

(10) At the end of the experiment, three groups of mice were dissected, and the liver tissue weight, white adipose tissue weight, kidney tissue weight, and spleen tissue weight, respectively, were visualized in fig. 9, 10, 11, and 12 (statistical significance was calculated using one-way anova and Dunnett's multiple comparison test with high fat diet as control group; P < 0.05; P < 0.01; P < 0.001). As can be seen from fig. 9 to 12, the weight of liver tissue, white adipose tissue, kidney tissue and spleen tissue was significantly reduced in the drug group compared to the high fat diet group, which indicates that the traditional Chinese medicine composition of the present invention can significantly alleviate the obesity effect of the high fat diet mice.

One dissected tissue organ of each mouse was selected from the three groups and observed as a section, and the liver tissue image, the liver tissue section image, the white fat section image, the kidney tissue section image, and the spleen tissue section image were shown in fig. 13, 14, 15, and 17, respectively, for three mice. As can be seen from fig. 13-17, the lipid content in liver, kidney and spleen of mice in the drug group was significantly reduced, and the size of adipocytes was significantly reduced, indicating that the traditional Chinese medicine composition of the present invention can promote lipid metabolism.

The specific effect of the extract obtained in example 1 was verified:

the extract obtained in the example 1 is prepared into oral liquid, and the effect of the water decoction obtained by the traditional water decoction method is compared with the effect of the traditional Chinese medicine composition prepared according to the weight ratio of the medicinal materials in the example 1.

Selecting 30 obese patients, wherein 15 patients take oral liquid prepared from the extract obtained in the example 1, the dosage of the oral liquid is 100mg/kg of the extract, and the oral liquid is marked as the group in the example 1; wherein 15 of the above-mentioned herbs were decocted with water (the weight of each herb was calculated according to the extraction ratio of example 1) and recorded as control group. Initial weights of 30 patients were recorded, and after the administration for 8 weeks, the weights of 30 patients were measured again to calculate the change in weight, and the results are shown in Table 9.

TABLE 930 weight change of obese patients

As can be seen from table 9, the extract obtained by the extraction method of the present invention has better weight-reducing effect compared to the conventional water decoction method. The reason is that the aqueous solutions with different pH values designed by the invention are close to the pH values of gastric juice and intestinal juice in a human body, and the extracted effective components are more beneficial to the absorption of the human body, so that the method has better effect compared with the traditional water decoction method.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. The weight-losing and sugar-reducing traditional Chinese medicine preparation is characterized by comprising the following raw materials in parts by weight: 25-30 parts of dried orange peel, 8-12 parts of cassia seed, 10-13 parts of houttuynia cordata, 19-24 parts of exocarpium benincasae, 9-12 parts of lotus pedicel, 10-12 parts of acanthopanax senticosus and 10-15 parts of phaseolus calcaratus;

the extraction method of the effective components in the weight-losing and blood-sugar-reducing traditional Chinese medicine preparation comprises the following steps: crushing and sieving the raw materials, extracting the raw materials by using an aqueous solution with the pH of 1.8-2.0, and filtering to obtain filter residue a; extracting the filter residue a with an aqueous solution with the pH value of 6.0-6.5, and filtering to obtain filter residue b; extracting the filter residue b by using an aqueous solution with the pH value of 8.5-9.0, and filtering; mixing the filtrates, and removing solvent from the supernatant to obtain extract.

2. The weight-losing and sugar-reducing traditional Chinese medicine preparation as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 25 parts of dried orange peel, 10 parts of cassia seed, 10 parts of heartleaf houttuynia herb, 20 parts of Chinese waxgourd peel, 10 parts of lotus pedicel, 10 parts of acanthopanax root and 15 parts of phaseolus calcaratus.

3. The preparation of claim 1 or 2, wherein the dosage form of the preparation is any one of oral liquid, granule, tablet or capsule.

4. The weight-losing and sugar-reducing traditional Chinese medicine preparation as claimed in claim 1, wherein the sieving is 20-40 mesh sieving.

5. The weight-losing and sugar-reducing traditional Chinese medicine preparation as claimed in claim 1, wherein the pH value of the aqueous solution with the pH value of 1.8-2.0 and the aqueous solution with the pH value of 6.0-6.5 are adjusted by hydrochloric acid; and adjusting the pH value of the water solution with the pH value of 8.5-9.0 by using sodium bicarbonate.

6. The weight-losing and sugar-reducing traditional Chinese medicine preparation as claimed in claim 1, wherein when the aqueous solution with the pH of 1.8-2.0 is used for extraction, the extraction time is 120-140 min, and the dosage of the aqueous solution is 10 times of the dry weight of the traditional Chinese medicine; when the extract is extracted by using an aqueous solution with the pH of 6.0-6.5, the extraction time is 60-70 min, and the dosage of the aqueous solution is 8 times of the dry weight of the traditional Chinese medicinal materials; when the traditional Chinese medicine is extracted by using an aqueous solution with the pH of 8.5-9.0, the extraction time is 30-35 min, and the dosage of the aqueous solution is 8 times of the dry weight of the traditional Chinese medicine.