CN113288052A

CN113288052A - Method for probing digestive tract tumor through FITC-UEA1 marker

Info

Publication number: CN113288052A
Application number: CN202110514647.6A
Authority: CN
Inventors: 蔺蓉
Original assignee: Union Hospital Tongji Medical College Huazhong University of Science and Technology
Current assignee: Tongji Medical College of Huazhong University of Science and Technology; Union Hospital Tongji Medical College Huazhong University of Science and Technology
Priority date: 2021-05-09
Filing date: 2021-05-09
Publication date: 2021-08-24

Abstract

The invention provides a method for exploring digestive tract tumor by FITC-UEA1 marking, which comprises the following steps: FITC-UEA1 labeling, modifying chitosan with vitex bean agglutinin capable of specifically binding with fucose on intestinal canal micro-ruffle cell, and constructing and researching particle oral delivery system or spraying locally in intestinal canal; the method comprises the following steps of (1) performing endoscopic system exploration, namely utilizing a confocal endoscopic system with the excitation wavelength of 495nm and the emission wavelength of 519nm to perform exploration, and receiving by an image display module; and (4) judging the tumor position, wherein an operator judges the tumor occurrence position according to the fluorescence intensity, and the fluorescence of the tumor position is reduced due to fucosylation. According to the method for exploring the tumor of the digestive tract by the FITC-UEA1 marker, the tumor of the digestive tract is specifically marked, and a confocal endoscope system is combined, so that the occurrence part of the tumor of the digestive tract can be simply, intuitively and accurately determined noninvasively according to the fluorescence intensity, the early discovery rate of the tumor of the digestive tract can be improved, the missed diagnosis rate and the fatality rate can be reduced, and the prognosis can be improved.

Description

Method for probing digestive tract tumor through FITC-UEA1 marker

Technical Field

The invention relates to the technical field of medical image identification, in particular to a method for exploring digestive tract tumors through FITC-UEA1 markers.

Background

Digestive system tumors are the most common tumor lesions in the world, seriously harm human health, and have profound significance for improving the survival rate of patients and lightening the social and economic burden by improving the diagnosis level of early tumors. Among the auxiliary methods for clinical early detection of digestive tract tumors, endoscopy is the most effective way for discovering digestive system tumors at present, but the current endoscopy lacks a specific identification method for tumor sites, and particularly finds the deficiency of tiny tumor lesions.

The Chinese invention patent CN201910519697.6 discloses an auxiliary method for clinical early detection of digestive tract tumors. However, the method is not targeted, digestive tract tumors cannot be clearly observed in many cases, a method capable of detecting the digestive tract tumors in a targeted mode is lacked at present, and the method needs to draw blood for examination and is invasive to patients.

Disclosure of Invention

The invention aims to improve the early discovery rate of the digestive tract tumor, reduce the missed diagnosis rate and the fatality rate and improve the prognosis by a method for identifying the digestive tract tumor part by probing FITC-UEA1 specific marker through an endoscope.

The invention also aims to determine the tumor part of the digestive tract by the fluorescence intensity under the endoscope, so as to carry out real-time accurate diagnosis, and the diagnosis method of the digestive tract tumor is simpler, more intuitive, noninvasive and accurate.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a method of probing digestive tract tumors by FITC-UEA1 labeling:

s1 labeling with FITC-UEA 1;

s2, exploring the endoscope system;

and S3, judging the tumor part.

Preferably, the labeling method in S1 is: chitosan is modified by the vitex negundo linn which can be specifically combined with fucose on intestinal tract micro-ruffled cells, and a particle oral delivery system is constructed and researched or local spraying is carried out on the intestinal tract.

Preferably, in S2, the probe is detected by a confocal endoscopic system with an excitation wavelength of 495nm and an emission wavelength of 519nm, and the probe is received by an image display module.

Preferably, in S3, the operator determines the tumor site based on the fluorescence intensity.

The invention has the following beneficial effects:

1. by observing FITC-UEA1 fluorescent dye which specifically reflects fucosylation level under an endoscope, tumor parts can be intuitively and accurately identified, the early discovery rate of digestive tract tumors is improved, and the missed diagnosis rate is reduced;

2. the digestive tract tumor is probed through FITC-UEA1 marking, the lesion part can be prompted without biopsy and histopathological examination, and the method is convenient and noninvasive.

Drawings

FIG. 1 is a flow chart of the steps of the method of the present invention for probing digestive tract tumors with FITC-UEA1 labeling.

FIG. 2 is an image of normal digestive tract probed using FITC-UEA1 labeling.

FIG. 3 is an imaging image of the detection of digestive tract tumors using FITC-UEA1 labeling.

Detailed Description

The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.

This invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather as being provided for the purpose of illustration and description. In the drawings, the size and relative sizes of structures and regions may be exaggerated for clarity.

As shown in fig. 1-3, the present invention provides a method for probing digestive tract tumors by FITC-UEA1 labeling, comprising the steps of:

s1 labeling with FITC-UEA 1;

s2, exploring the endoscope system;

and S3, judging the tumor part.

This scheme is further illustrated below with reference to specific examples:

the first embodiment is as follows:

FITC-UEA labeling, namely modifying chitosan by using vitex negundo linn capable of being specifically combined with fucose on intestinal tract micro-ruffle cells, and constructing and researching a particle oral delivery system or locally spraying the particle in the intestinal tract.

And 2, probing by using an endoscope system with the excitation wavelength of 495nm and the emission wavelength of 519nm, and receiving and displaying the analysis result by using an image display module.

And 3, judging the tumor occurrence part by an operator according to the fluorescence intensity, wherein the fluorescence of the tumor occurrence part is increased due to the increase of fucosylation.

FIG. 2 is an image of normal digestive tract probed using FITC-UEA1 labeling.

By comparing the imaging results of the two marker sets, it can be found that: the tumor part can be specifically identified by observing FITC-UEA1 fluorescent dye which specifically reflects fucosylation level under an endoscope, the FITC-UEA1 fluorescence of the tumor part is obviously reduced, and the morphological structure of the tissue reflected by the fluorescent dye is obviously disordered. Therefore, the method of labeling by FITC-UEA1 can improve the detection rate of tumors and accurately identify the lesion sites. On the other hand, compared with the pathological diagnosis, the method is more convenient and noninvasive through the tissue biopsy and the experimental method for probing the digestive tract tumor by FITC-UEA1 marking.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.

Claims

1. A method for probing digestive tract tumors by FITC-UEA1 labeling, comprising the steps of:

s1 labeling with FITC-UEA 1;

s2, exploring the endoscope system;

and S3, judging the tumor part.

2. A method for probing tumors of the digestive tract by FITC-UEA1 labeling according to claim 1, characterized in that: the marking method in the S1 comprises the following steps: chitosan is modified by the vitex negundo linn which can be specifically combined with fucose on intestinal tract micro-ruffled cells, and a particle oral delivery system is constructed and researched or local spraying is carried out on the intestinal tract.

3. A method for probing tumors of the digestive tract by FITC-UEA1 labeling according to claim 1, characterized in that: in the S2, a confocal endoscope system with the excitation wavelength of 495nm and the emission wavelength of 519nm is used for detection, and an image display module receives the detection.

4. A method for probing tumors of the digestive tract by FITC-UEA1 labeling according to claim 1, characterized in that: in S3, the operator determines the tumor site based on the fluorescence intensity.