CN113278562A - Soil improvement microbial agent for cherry planting - Google Patents
Soil improvement microbial agent for cherry planting Download PDFInfo
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- CN113278562A CN113278562A CN202110721595.XA CN202110721595A CN113278562A CN 113278562 A CN113278562 A CN 113278562A CN 202110721595 A CN202110721595 A CN 202110721595A CN 113278562 A CN113278562 A CN 113278562A
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- 241000167854 Bourreria succulenta Species 0.000 title claims abstract description 124
- 235000019693 cherries Nutrition 0.000 title claims abstract description 124
- 239000002689 soil Substances 0.000 title claims abstract description 47
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 34
- 230000000813 microbial effect Effects 0.000 title claims abstract description 31
- 230000006872 improvement Effects 0.000 title claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 17
- 241000219357 Cactaceae Species 0.000 claims abstract description 16
- 241000235015 Yarrowia lipolytica Species 0.000 claims abstract description 16
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 239000004480 active ingredient Substances 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 40
- 230000004151 fermentation Effects 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 21
- 239000001963 growth medium Substances 0.000 claims description 11
- 239000003516 soil conditioner Substances 0.000 claims description 11
- 235000013312 flour Nutrition 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 240000000111 Saccharum officinarum Species 0.000 claims description 5
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 239000002068 microbial inoculum Substances 0.000 claims description 5
- 238000011218 seed culture Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 238000005336 cracking Methods 0.000 abstract description 22
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 238000004321 preservation Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 2
- 235000013399 edible fruits Nutrition 0.000 description 49
- 238000002474 experimental method Methods 0.000 description 8
- 238000005259 measurement Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 4
- 241001233061 earthworms Species 0.000 description 3
- 210000003128 head Anatomy 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002366 lipolytic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention belongs to the technical field of agricultural planting and soil microorganism, and particularly relates to a soil improvement microbial agent for cherry planting and a preparation method and application thereof. The active ingredients of the microbial agent are candida lipolytica, bacillus licheniformis and cactusThe ratio of the effective viable count of the candida lipolytica, the bacillus licheniformis, the cactus bacillus and the photosynthetic bacteria is 1: 2: 2: 2, the viable count of the soil improvement microbial agent is 3-10 multiplied by 109one/mL. The compound microbial agent disclosed by the invention improves cherry planting soil, reduces the cherry cracking rate, improves the cherry hardness, prolongs the cherry preservation time, and improves the cherry yield and quality.
Description
Technical Field
The invention belongs to the technical field of agricultural planting and soil microorganism, and particularly relates to a soil improvement microbial agent for cherry planting prepared by a mixed fermentation method with strong flora survival and propagation adaptability, and a preparation method and application thereof.
Background
The cherries are collectively called as plants of Rosaceae and Oriental, have high economic value and are one of the main planted fruits in the Dalian connection region. The problems of high fruit cracking rate, low sweetness and hardness and short preservation time exist in the existing cherry planting process, and the problems of soil hardening and fertility decline usually exist in the planted soil, so that the yield and quality of cherry fruits are influenced; the utilization rate of nitrogen, phosphorus and potassium used in the planting is low. The invention improves the soil to improve the quality of the planted cherries.
Disclosure of Invention
The invention aims to provide a compound nutritional type soil conditioner with strong adaptability to survival and propagation of flora, which is suitable for cherry planting, aiming at the problems of soil hardening and fertility decline in cherry planting.
The purpose of the invention is achieved by the following technical scheme:
the invention provides a soil improvement microbial agent for cherry planting, which comprises the active ingredients of candida lipolytica, bacillus licheniformis, cactus bacillus and photosynthetic bacteria, wherein the effective viable count ratio of the candida lipolytica, the bacillus licheniformis, the cactus bacillus and the photosynthetic bacteria is 1: 2: 2: 2, the number of viable bacteria of the soil conditioner is 3-10 multiplied by 109one/mL.
In the technical scheme, furthermore, the viable count of the soil conditioner is 5-8 multiplied by 109one/mL.
The invention also provides a preparation method of the soil improvement microbial agent for cherry planting, which comprises the following steps:
(1) preparing a seed solution: respectively inoculating candida lipolytica, bacillus licheniformis, cactus bacillus and photosynthetic bacteria which are cultured in an inclined plane into a seed culture medium, and culturing at 27-38 ℃ to respectively obtain seed solutions of the candida lipolytica, the bacillus licheniformis, the cactus bacillus and the photosynthetic bacteria;
(2) liquid state fermentation culture: respectively transferring the seed solutions obtained in the step (1) into a fermentation culture medium according to the volume ratio of 5-10%, carrying out fermentation culture for 96-168 h at 27-38 ℃, and respectively obtaining a candida lipolytica fermentation liquid, a bacillus licheniformis fermentation liquid, a cactus fermentation liquid and a photosynthetic bacteria fermentation liquid; and mixing the fermentation liquids to obtain the microbial agent.
In the above technical solution, further, the seed culture medium of step (1) is: 200kg of sugarcane brown sugar, 3kg of wheat flour and 2kg of soybean powder, adding water to 900L, and adjusting the pH to 7.0-7.5;
the fermentation medium of the step (2) is as follows: 200kg of sugarcane brown sugar, 3kg of wheat flour and 2kg of soybean powder, adding water to 900L, and adjusting the pH to 7.0-7.5;
the particle size of the soybean flour is 50-300 meshes.
In the above technical scheme, further, the number of effective viable bacteria in each of the fermentation broth of the lipolytic candida saccharomycetes, the bacillus licheniformis fermentation broth, the cactus fermentation broth and the photosynthetic bacteria fermentation broth is 3-10 × 108one/mL.
The third aspect of the invention provides a use method of the soil improvement microbial agent for cherry planting, which comprises the following steps: in a fruiting period, 2-5 kg of the microbial inoculum is used for each tree, and when the microbial inoculum is used, the microbial inoculum is uniformly dripped or irrigated within 1 meter of the radius around the cherry trees.
In the above technical solution, further, the one result period is: and (5) cultivating in the greenhouse: ten days from 9 months to 5 months of the next year to 6 months; the natural result of the earth: from middle of 2 months to middle of 6 months in the year.
Compared with the prior art, the invention has the beneficial effects that:
by using the compound microbial agent disclosed by the invention to improve cherry planting soil, the cherry cracking rate is reduced, the cherry hardness is improved, the cherry preservation time is prolonged, the cherry yield is high, and the quality is improved.
The culture medium used in the microbial inoculum preparation process can ensure that the strains are stably fermented, the fermentation efficiency is high, and the number of the bacteria in the fermentation liquid reaches 3-10 multiplied by 10 within 96 hours of fermentation9The effective viable count per mL can achieve the purpose of rapid culture, the same culture medium can ferment different strains, and the composition of the culture medium is less, thereby further reducing the cost.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but are not intended to limit the invention thereto. In the following examples, unless otherwise specified, the experimental methods used were all conventional methods, and the species, reagents and the like used were all available from biological or chemical reagents companies.
Example 1 preparation of soil improving microbial Agents
(1) Respectively inoculating candida lipolytica, bacillus licheniformis, cactus bacillus and photosynthetic bacteria which are cultured in an inclined plane into a seed culture medium, and culturing at 27-38 ℃ to respectively obtain seed solutions of the candida lipolytica, the bacillus licheniformis, the cactus bacillus and the photosynthetic bacteria;
(2) liquid state fermentation culture: respectively transferring the various seed liquids obtained in the step (1) into a fermentation culture medium according to the volume ratio of 5%, fermenting at the temperature of 27-38 ℃ under the condition that dissolved oxygen is more than or equal to 20%, and carrying out fermentation culture for 96-168 h to respectively obtain candida lipolytica fermentation liquid, bacillus licheniformis fermentation liquid, cactus fermentation liquid and photosynthetic bacteria fermentation liquid; mixing the fermentation liquor to obtain the soil improvement microbial agent, wherein the viable count of the soil improvement microbial agent is 5-8 multiplied by 109Per mL;
the seed (fermentation) media used were: 200kg of sugarcane brown sugar, 3kg of wheat flour and 2kg of soybean meal, adding water to 900L, adjusting the pH to 7.0-7.5, and sterilizing at 121 ℃ for 30 min.
Example 2
The experimental site: ron shun Jia village
Subject: cherry (Mingzhu)
Grouping processing:
(1) blank control group: common planting soil
(2) Experimental groups: example 1 preparation of microbial Agents for soil improvement of the present invention
The experimental method comprises the following steps:
selecting 16 cherry trees with equivalent yield (average yield is 45 kg/cherry tree), and dividing the cherry trees into a blank control group and an experimental group, wherein the cherry trees between the two groups are separated by a certain distance.
In the 1 st decade of 2018, the soil improvement microbial agent prepared in example 1 is applied to soil of an experimental group, dispersed to a circular area with the diameter of 1m below cherry trees, and 3kg of soil improvement microbial agent liquid is dripped or irrigated to each tree, and other planting conditions are the same as those of a control group.
After 60 days, the soil was observed.
Compared with the soil of a control group, the soil of the experimental group can be loosened by naked eyes and earthworms move.
The soil improvement microbial agent of example 1 was further applied, and after the cherries were ripened in late 4 months, the sour-sweetness, hardness, fruit cracking rate, and the like of the cherries were compared.
1. Cherry firmness
Picking cherries of a control group and a test group respectively, randomly picking the cherries from 4 cherry trees of each group, picking 50 cherries respectively, measuring the hardness of the cherries, and taking an average value. And (3) using a handheld hardometer to peel off a thin layer of the cherry, wherein the peeling area is slightly larger than the area of the lateral head of the hardometer. The results of the measurement were as follows:
| control group | Experimental group | |
| Hardness kg/m2 | 2.51a | 3.95a |
As can be seen from the results in the table above, the experimental group has better hardness and is more convenient to store and transport.
2. Cracking rate of cherry
The fruit cracking rate is equal to the number of the cracked fruits/the total number of the fruits multiplied by 100 percent.
Picking cherries of a control group and a test group respectively, randomly picking the cherries from 4 cherry trees of each group, picking 200 cherries respectively, and counting the fruit cracking rate. The results of the measurement were as follows:
| control group | Experimental group | |
| Percentage of fruit cracking% | 42 | 8 |
As can be seen from the above table, the fruit cracking rate of the experimental group is significantly lower than that of the control group.
3. Cherry yield increase
The number of cherries (the cherries with fruit cracks removed) of the control group and the experimental group are counted respectively, and the average yield of each cherry tree of the cherries of the experimental group and the control group is calculated.
| Control group | Experimental group | |
| Yield (kilogram per plant) | 32 | 40 |
4. Picking cherries of a control group and cherries of an experimental group respectively, picking 50 cherries respectively, selecting non-damaged fruits for picking, putting the picked fruits in a hollow frame respectively, putting the fruits in a shade place, and storing the fruits under the same condition.
| Number of rotten control group | Experiment group rotten number (number) | |
| 5 days | 2 | 0 |
| 9 days | 30 | 2 |
| 15 days | 50 | 6 |
After 5 days, the control group begins to rot, the experimental group does not rot fruits, after 9 days, the control group rot in a large area, the experimental group only rot individually, after 15 days, the control group all rot, and the rotten fruits of the experimental group are counted to have about 10% rot.
Therefore, the fresh-keeping time of the cherries of the experimental group is obviously longer than that of the cherries of the control group, and the cherries can be stored for a long time of 9-15 days.
5. Fruit grower feedback
According to the feedback of the planting experience of the fruit growers for many years, after the soil conditioner is used, the cherry glossiness, the hand feeling, the taste and the fruit cracking condition are all improved, the feedback is good after the fruit growers eat the soil conditioner, and the number of customers is increased obviously.
Example 3
The experimental site: jinzhou seven-top mountain street little Zhujiacun
Subject: cherry (Sha Mi bean)
Grouping processing:
(1) blank control group: common planting soil
(2) Experimental groups: soil improving agent of the present invention prepared in example 1
The experimental method comprises the following steps:
16 cherry trees with equal yield (average yield of 45 kg/cherry) are selected and divided into a blank control group and an experimental group, and the cherry trees between the two groups are separated by a certain distance.
In 1/last month of 2019, the soil conditioner prepared in example 1 was applied to soil of an experimental group, dispersed in a circular area with a diameter of 1m under cherry trees, and 3kg of soil conditioner solution was dripped or irrigated to each tree, and other planting conditions were the same as those of a control group.
After 60 days, the soil was observed.
Compared with the soil of a control group, the soil of the experimental group can be loosened by naked eyes and earthworms move.
The soil improvement microbial agents of example 1 were continuously applied, and after the cherries matured in 4 th of the month, the hardness and the fruit cracking rate of the cherries were compared.
1. Cherry firmness
Picking cherries of a control group and a test group respectively, randomly picking the cherries from 4 cherry trees of each group, picking 50 cherries respectively, measuring the hardness of the cherries, and taking an average value. And (3) using a handheld hardometer to peel off a thin layer of the cherry, wherein the peeling area is slightly larger than the area of the lateral head of the hardometer. The results of the measurement were as follows:
| control group | Experimental group | |
| Hardness kg/m2 | 2.15a | 3.87a |
As can be seen from the results in the table above, the experimental group has better hardness and is more convenient to store and transport.
2. Cracking rate of cherry
The fruit cracking rate is equal to the number of the cracked fruits/the total number of the fruits multiplied by 100 percent.
Picking cherries of a control group and a test group respectively, randomly picking the cherries from 4 cherry trees of each group, picking 200 cherries respectively, and counting the fruit cracking rate. The results of the measurement were as follows:
| control group | Experimental group | |
| Percentage of fruit cracking% | 51 | 9 |
As can be seen from the above table, the fruit cracking rate of the experimental group is significantly lower than that of the control group.
3. Cherry yield increase
The number of cherries (the cherries with fruit cracks removed) of the control group and the experimental group are counted respectively, and the average yield of each cherry tree of the cherries of the experimental group and the control group is calculated.
| Control group | Experimental group | |
| Yield (kilogram per plant) | 31 | 39 |
4. Picking cherries of a control group and cherries of an experimental group respectively, picking 50 cherries respectively, selecting non-damaged fruits for picking, putting the picked fruits in a hollow frame respectively, putting the fruits in a shade place, and storing the fruits under the same condition.
| Number of rotten control group | Experiment group rotten number (number) | |
| 5 days | 8 | 0 |
| 9 days | 35 | 4 |
| 13 days | 50 | 10 |
After 5 days, the control group begins to rot, the experimental group does not rot fruits, after 9 days, the control group rot in a large area, the experimental group only rot individually, after 13 days, the control group all rot, and the rotten fruits of the experimental group are counted to have about 20% rot.
Therefore, the fresh-keeping time of the cherries of the experimental group is obviously longer than that of the cherries of the control group.
Example 4
The experimental site: fort village chenchen tun
Subject: cherry (Russia No. 8 cherry)
Grouping processing:
(1) blank control group: common planting soil
(2) Experiment group: soil improving agent of the present invention prepared in example 1
The experimental method comprises the following steps:
selecting 16 cherry trees with equivalent yield (average yield is 50 kg/cherry tree), and dividing the cherry trees into a blank control group and an experimental group, wherein the cherry trees in the two groups are separated by a certain distance.
In 1/last decade of 2019, the soil conditioner prepared in example 1 is applied to soil of an experimental group, dispersed to a circular area with the diameter of 1m below cherry trees, and drip-irrigated or irrigated with soil conditioner bacteria liquid, 2-5 kg of each tree is planted, and other planting conditions are the same as those of a control group.
After 60 days, the soil was observed.
Compared with the soil of a control group, the soil of the experimental group can be loosened by naked eyes and earthworms move.
The soil improvement agent of example 1 was further applied, and after the cherries were ripe in the middle of 4 months, the hardness, fruit cracking rate, and the like of the cherries were compared.
1. Cherry firmness
Picking cherries of a control group and a test group respectively, randomly picking the cherries from 4 cherry trees of each group, picking 50 cherries respectively, measuring the hardness of the cherries, and taking an average value. And (3) using a handheld hardometer to peel off a thin layer of the cherry, wherein the peeling area is slightly larger than the area of the lateral head of the hardometer. The results of the measurement were as follows:
| control group | Experimental group | |
| Hardness kg/m2 | 2.76a | 4.25a |
As can be seen from the results in the table above, the experimental group has better hardness and is more convenient to store and transport.
2. Cracking rate of cherry
The fruit cracking rate is equal to the number of the cracked fruits/the total number of the fruits multiplied by 100 percent.
Picking cherries of a control group and a test group respectively, randomly picking the cherries from 4 cherry trees of each group, picking 200 cherries respectively, and counting the fruit cracking rate. The results of the measurement were as follows:
| control group | Experimental group | |
| Percentage of fruit cracking% | 60 | 11 |
As can be seen from the above table, the fruit cracking rate of the experimental group is significantly lower than that of the control group.
3. Cherry yield increase
The number of cherries (the cherries with fruit cracks removed) of the control group and the experimental group are counted respectively, and the average yield of each cherry tree of the cherries of the experimental group and the control group is calculated.
| Control group | Experimental group | |
| Yield (kilogram per plant) | 32 | 45 |
4. Picking cherries of a control group and cherries of an experimental group respectively, picking 50 cherries respectively, selecting non-damaged fruits for picking, putting the picked fruits in a hollow frame respectively, putting the fruits in a shade place, and storing the fruits under the same condition.
| Number of rotten control group | Experiment group rotten number (number) | |
| 5 days | 9 | 0 |
| 9 days | 38 | 5 |
| 15 days | 50 | 12 |
After 5 days, the control group begins to rot, the experimental group does not rot fruits, after 9 days, the control group rot in a large area, the experimental group only rot individually, after 15 days, the control group all rot, and the rotten fruits of the experimental group are counted to have about 20% rot.
Therefore, the fresh-keeping time of the cherries of the experimental group is obviously longer than that of the cherries of the control group.
It will be apparent to those skilled in the art from this disclosure that many changes and modifications can be made, or equivalents modified, in the embodiments of the invention without departing from the scope of the invention. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention shall still fall within the protection scope of the technical solution of the present invention, unless the contents of the technical solution of the present invention are departed.
Claims (7)
1. The soil improvement microbial agent for cherry planting is characterized in that the active ingredients of the microbial agent are candida lipolytica, bacillus licheniformis, cactus bacillus and photosynthetic bacteria, and the proportion of the effective viable count of the candida lipolytica, the bacillus licheniformis, the cactus bacillus and the photosynthetic bacteria is 1: 2: 2: 2, the number of viable bacteria of the soil conditioner is 3-10 multiplied by 109one/mL.
2. The cherry planting microbial agent as claimed in claim 2, wherein the viable count of the soil conditioner is 5-8 x 109one/mL.
3. The preparation method of the cherry planting microbial agent for improving soil, which is described in claim 1, is characterized by comprising the following steps:
(1) preparing a seed solution: respectively inoculating candida lipolytica, bacillus licheniformis, cactus bacillus and photosynthetic bacteria which are cultured in an inclined plane into a seed culture medium, and culturing at 27-38 ℃ to respectively obtain seed solutions of the candida lipolytica, the bacillus licheniformis, the cactus bacillus and the photosynthetic bacteria;
(2) liquid state fermentation culture: respectively transferring the seed solutions obtained in the step (1) into a fermentation culture medium according to the volume ratio of 5-10%, carrying out fermentation culture for 96-168 h at 27-38 ℃, and respectively obtaining a candida lipolytica fermentation liquid, a bacillus licheniformis fermentation liquid, a cactus fermentation liquid and a photosynthetic bacteria fermentation liquid; and mixing the fermentation liquids to obtain the microbial agent.
4. The preparation method of the cherry planting microbial agent for improving soil according to claim 3, wherein the seed culture medium in the step (1) is: 200kg of sugarcane brown sugar, 3kg of wheat flour and 2kg of soybean powder, adding water to 900L, and adjusting the pH to 7.0-7.5;
the fermentation medium of the step (2) is as follows: 200kg of sugarcane brown sugar, 3kg of wheat flour and 2kg of soybean powder, adding water to 900L, and adjusting the pH to 7.0-7.5;
the particle size of the soybean flour is 50-300 meshes.
5. The method for preparing a soil improvement microbial agent for cherry planting according to claim 3, wherein the number of effective viable bacteria in each fermentation broth of the Candida lipolytica fermentation broth, the Bacillus licheniformis fermentation broth, the Cactus bacillus fermentation broth and the photosynthetic bacteria fermentation broth is 3-10 x 108one/mL.
6. The use method of the cherry planting soil improvement microbial agent as claimed in claim 1, wherein the method comprises the following steps: in a fruiting period, 2-5 kg of the microbial agent is used for each tree, and when the microbial agent is used, the microbial agent is uniformly dripped or irrigated within 1 meter of the radius around the cherry trees.
7. The use method of the cherry planting microbial inoculum according to claim 6, wherein the one fruiting cycle is: and (5) cultivating in the greenhouse: ten days from 9 months to 5 months of the next year to 6 months;
the natural result of the earth: from middle of 2 months to middle of 6 months in the year.
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