CN113265318A

CN113265318A - Separation and purification device of bacterial strain for preparing biosurfactant

Info

Publication number: CN113265318A
Application number: CN202110663911.2A
Authority: CN
Inventors: 陈磊; 金大鹏; 张树林; 吴志广; 郭章; 向冬雨
Original assignee: Shenzhen Hengwei Technology Co ltd
Current assignee: Shenzhen Hengwei Technology Co ltd
Priority date: 2021-06-16
Filing date: 2021-06-16
Publication date: 2021-08-17

Abstract

The invention discloses a separation and purification device of a strain for preparing a biosurfactant, which comprises a vibration unit capable of vibrating up and down, a drawing unit capable of moving up and down, an inoculation unit, a sealing moving unit, a separation unit and a storage unit; the discharge gate of vibrations unit corresponds with the feed inlet of drawing the unit, the discharge gate of drawing the unit corresponds with the feed inlet of inoculation unit, the discharge gate of inoculation unit corresponds with the feed inlet of sealed mobile unit, the discharge gate of separation unit corresponds with the feed inlet of preserving the unit.

Description

Separation and purification device of bacterial strain for preparing biosurfactant

Technical Field

The invention relates to the field of biosynthesis, in particular to a separation and purification device of a strain for preparing a biosurfactant.

Background

The biosurfactant is a microbial surfactant with various structures, and comprises glycolipid, lipopeptide, lipoprotein, phospholipid, neutral lipoid derivative and the like. Surfactants are generally composed of a hydrophilic group and a hydrophobic group. The rhamnolipid belongs to an anionic biosurfactant of glycolipids, has good performances of washing, emulsification, solubilization, wetting, permeation and the like, and has good biodegradability. Has wide application prospect in the fields of biological energy, petrochemical industry, biological medicine, food, daily chemical industry and the like.

Inoculation is one of the key links in the production of edible fungi, and is also called seeding. If the inoculation technology is not over-closed, the mixed bacteria pollution can be caused, the survival rate is low, the previous work is abandoned, and the cultivation is not mentioned. Whether the strain is separated, passaged or propagated, the strain is strictly carried out according to the aseptic operation protocol under the aseptic condition. The key points are as follows: personnel quality, environmental disinfection, space disinfection, disinfection of carried objects, sterilization and inoculation.

In the traditional strain inoculation operation, experimenters need to operate in a sterile environment, and cannot ensure complete operation in the environment due to personnel difference, so that the yield, the yield and the purity of the strain are influenced.

Disclosure of Invention

Based on the defects in the prior art mentioned in the background technology, the invention provides a device for separating and purifying the bacterial strain for preparing the biosurfactant.

The invention overcomes the technical problems by adopting the following technical scheme, and specifically comprises the following steps: a separation and purification device of bacterial strains for preparing a biosurfactant comprises a vibration unit capable of vibrating up and down, a drawing unit capable of moving up and down, an inoculation unit, a sealing moving unit, a separation unit and a storage unit;

the discharge gate of vibrations unit corresponds with the feed inlet of drawing the unit, the discharge gate of drawing the unit corresponds with the feed inlet of inoculation unit, the discharge gate of inoculation unit corresponds with the feed inlet of sealed mobile unit, the discharge gate of separation unit corresponds with the feed inlet of preserving the unit.

As a further scheme of the invention, the vibration unit comprises a first mounting plate, a vibration plate, a first slide rod, a first spring, a first electric telescopic rod, a positioning cylinder, a mixing pipe, a sealing cover plate, a first chute, a first slide block, a second spring, a first vertical plate, a first cam, a first motor and a clamping assembly;

a plurality of first sliding rods are fixed on the upper end face of the first mounting plate, one ends of the first sliding rods, far away from the first mounting plate, slide through the vibration plate and are fixed with a limiting block, a plurality of first springs are sleeved on the outer walls of the first sliding rods, one ends of the first springs are fixed on the upper end face of the first mounting plate, the other ends of the first springs are fixed on the lower end face of the vibration plate, a positioning cylinder is fixed on the upper end face of the vibration plate, the lower end of the mixing tube is slidably sleeved in the positioning cylinder, the upper end of the mixing tube is located on the lower end face of the sealing cover plate, the first vertical plate is vertically fixed on the upper end face of the vibration plate, a first sliding groove is formed in the side wall of the vibration plate, close to the positioning cylinder, in the vertical direction, one end of the first sliding block is slidably arranged in the first sliding groove, the other end of the first sliding groove extends out to be fixed with the sealing cover plate, a second spring is arranged in the sealing cover plate, one end of the second spring is fixed on the lower end face of the sealing cover plate, the other end of the first electric telescopic rod is fixed on the inner wall of the first sliding groove, the first electric telescopic rod is fixed on the side wall of the first vertical plate, the telescopic end of the first electric telescopic rod is far away from the first vertical plate and is fixed with the outer wall of the positioning cylinder, the first motor is fixed on the upper end face of the first mounting plate, the output shaft of the first electric telescopic rod is fixed with the first cam, the protruding part of the first cam is in sliding contact with the lower end face of the vibration plate, and the first vertical plate is provided with a clamping assembly used for locking the opening end of the sealing cover plate and the opening end of the mixing pipe.

As a further scheme of the present invention, the clamping assembly includes a first through groove, a second through groove, a first telescopic rod, a first fixing plate, a second fixing plate, a first sleeve, a second sliding rod, a third spring, a positioning hole, a second telescopic rod, a fourth spring, a fifth spring, a first push plate, a first hinge rod, and a second hinge rod;

the first vertical plate is provided with a first through groove and a second through groove which are oppositely arranged, a first fixing plate and a second fixing plate are respectively arranged in the first through groove and the second through groove in a sliding mode, the positioning cylinder is located between the first fixing plate and the second fixing plate, second sliding rods are respectively arranged on opposite surfaces of the first fixing plate and the second fixing plate in a sliding mode, one ends, adjacent to the second sliding rods, are respectively arc-shaped, the other ends respectively penetrate through the first fixing plate and the second fixing plate in a sliding mode and are sleeved with first sleeves in a sliding mode, the two first sleeves are respectively fixed on the side walls of the first fixing plate and the second fixing plate, third springs are respectively arranged in the two first sleeves, one ends of the third springs are fixed on the inner wall of the first sleeve, the other ends of the third springs are fixed at one ends, slidably sleeved with the second sliding rods and the first sleeve, and the sealing cover plate and the first fixing plate, Two adjacent side walls of the second fixing plate are respectively provided with positioning holes with arc-shaped ends clamped with the second sliding rod, one ends of the first fixing plate and the second fixing plate which are respectively arranged in the first through groove and the second through groove in a sliding manner are respectively hinged with one ends of the first hinge rod and the second hinge rod, the other ends of the first hinge rod and the second hinge rod are respectively hinged on the side wall of the first push plate, the first telescopic rod is fixed on the side wall of the first vertical plate, the telescopic end of the first telescopic rod is fixed on the side wall of the push plate, the telescopic end of the first telescopic rod is fixed on the side wall of the first vertical plate, the other end of the first telescopic rod is fixed on the side wall of the first push plate, the second telescopic rod is fixed in the first through groove and the second through groove, and the telescopic ends of the two second telescopic rods are respectively fixed on the side walls of the first fixing plate and the second fixing plate, and a fifth spring is sleeved on the outer wall of the second telescopic rod.

As a further aspect of the present invention, the drawing unit includes a second mounting plate, a second vertical plate, a third through groove, a second electric telescopic rod, a third mounting plate, a moving plate, a drawing device, a piston plate, a drawing tube, a scraping rod, a connecting plate, a first threaded rod, a first threaded sleeve, a second motor, a first bevel gear set, a second bevel gear set, a third bevel gear set, a first gear, a second gear, a first transmission rod, a second transmission rod, a third vertical plate, and a fourth mounting plate;

the second vertical plate and the third vertical plate are oppositely arranged and fixed on the upper end surface of the second mounting plate, a third through groove is formed in the second vertical plate along the vertical direction, the fourth mounting plate is positioned above the second mounting plate and fixed between the second vertical plate and the third vertical plate, a first thread sleeve is rotatably arranged at one end of the connecting plate, a moving plate is fixedly arranged at the other end of the connecting plate in a sliding way and penetrates through the third through groove, a suction device is rotatably arranged on the moving plate, a piston plate is movably arranged in the suction device, the third mounting plate is fixed on the upper end surface of the moving plate through a mounting frame, a second electric telescopic rod is fixed on the lower end surface of the third mounting plate, the telescopic end of the connecting plate hermetically and slidably penetrates through the upper end of the suction device and is fixed with the piston plate, the lower end of the suction device is communicated with the upper end of the suction tube, a first gear is further sleeved on the outer wall of the suction device, and the scraping rod is horizontally positioned under the lower end of the suction tube and rotatably connected with the outer wall through a support rod, first threaded rod one end is rotated and is set up at second mounting panel up end, and the other end rotates and passes the fourth mounting panel and is connected with the output shaft transmission who fixes the second motor at fourth mounting panel up end through first bevel gear group, first threaded rod and first thread bush screw thread registrate, the output shaft and the first transfer line one end of second motor rotate the registrate, and the registrate department is provided with the bolt, the other end of first transfer line is connected with second transfer line one end transmission through second bevel gear group, the other end of second transfer line is connected with the one end transmission of third transfer line through third bevel gear group, the other end of third transfer line is fixed with the second gear with first gear engagement, the ware of drawing is located the stroke that the location section of thick bamboo removed.

As a further scheme of the invention, the inoculation unit comprises a fourth vertical plate, a first baffle, a second push plate, a third slide bar, a sixth spring, a culture dish, a second chute, a fourth through groove, a U-shaped sliding plate, a connecting rod, a second threaded sleeve, a second baffle, a fourth bevel gear set and a third motor;

the first baffle plate and the third vertical plate are oppositely arranged and fixed on the upper end surface of the second mounting plate, two oppositely arranged fourth vertical plates are arranged between the first baffle plate and the third vertical plates, the second push plate is slidably arranged on the upper end surface of the second mounting plate and is positioned between the oppositely arranged fourth vertical plates, one end of the third slide bar is fixed on one side wall of the second push plate, the other end of the third slide bar is slidably sleeved with a sixth spring and slidably penetrates through the third vertical plate, one end of the sixth spring is fixed on the side wall of the second push plate, the other end of the sixth spring is fixed on the side wall of the third vertical plate, the other side wall of the second push plate is contacted with the outer wall of the culture dish, a plurality of culture dishes can be arranged between the two oppositely arranged fourth vertical plates along the sliding direction of the third slide bar, the culture dishes are slidably arranged on the upper end surface of the second mounting plate, and a second chute is arranged on the upper end surface of the second mounting plate along the sliding direction vertical bar perpendicular to the third slide bar, the improved structure of the screw extractor is characterized in that a fourth through groove is further formed in the second sliding groove, the U-shaped sliding plate is arranged in the second sliding groove in a sliding mode, the opening of the U-shaped sliding plate is communicated with a channel between two oppositely arranged fourth vertical plates, the extractor is located on the stroke of left and right movement of the U-shaped sliding plate, one end of the connecting rod is fixed to the lower end face of the U-shaped sliding plate, the other end of the connecting rod penetrates through the fourth through groove in a sliding mode and is fixed with a second threaded sleeve, two ends of a second threaded rod are respectively rotatably arranged on two supporting plates fixed to the lower end face of the second mounting plate, the second threaded rod is in threaded sleeve fit with the second threaded sleeve, the second threaded rod is further in transmission connection with an output shaft of a third motor through a fourth bevel gear set, and a second baffle plate used for opening and closing the channel between the two fourth vertical plates is fixed to one limiting vertical plate of the U-shaped sliding plate through a mounting piece.

As a further scheme of the invention, the sealing moving unit comprises a fifth vertical plate, a mounting box, a fourth sliding rod, a seventh spring, a third push plate, a fixing frame, a through hole, a fifth sliding rod, an eighth spring, a sixth sliding rod, a ninth spring, a hinged plate, a fifth through groove, a first cylinder, an L-shaped push plate, a first conveyor belt, a first belt, a second sleeve, a fourth conveyor rod, a culture dish cover and a fifth bevel gear set;

the mounting box is fixed on the upper end surfaces of two fifth vertical plates which are oppositely arranged, a third push plate is movably arranged in the mounting box, one side wall of the third push plate is connected with one end of a fourth slide bar, the other end of the fourth slide bar is sleeved with a seventh spring and slides out of the side wall of the mounting box, one end of the seventh spring is fixed on the side wall of the third push plate, the other end of the seventh spring is fixed on the inner wall of the mounting box, the other side wall of the third push plate is contacted with the outer wall of the culture dish cover, a through hole matched with the culture dish cover is formed in a bottom plate of the mounting box, fifth slide bars are arranged on the two fifth vertical plates which are oppositely arranged in a sliding mode, one ends of the two fifth slide bars are positioned right below the through hole, the other ends of the two fifth slide bars respectively slide through the fifth vertical plates and are sleeved with eighth springs, one ends of the eighth springs are fixed with a stop block fixed at one end of the fifth slide bars, and the other ends of the eighth springs are fixed with the side walls of the fifth vertical plates, the hinged plate is hinged on the upper end surface of the mounting box, one end of the hinged plate extends out of the mounting box, the other end of the hinged plate is positioned on the upper end surface of the mounting box and is provided with a fifth through groove, one end of a sixth sliding rod penetrates through a fixed frame fixed on the upper end surface of the mounting box in a sliding manner, a limiting plate is fixed on the other end of the sixth sliding rod and penetrates into the mounting box through the fifth through groove in a sliding manner, a ninth spring is sleeved on the sixth sliding rod, one end of the ninth spring is fixed on the lower end surface of the fixed frame, the other end of the ninth spring is fixed on the upper end surface of the limiting plate, a first air cylinder is fixed on the upper end surface of a second mounting plate, an L-shaped push plate is fixed on the telescopic end of the first air cylinder, a short plate of the L-shaped push plate is in sliding fit with one end of the hinged plate extending out of the mounting box, a first conveying belt is rotatably arranged between two fifth vertical plates which are arranged oppositely through two rotating shafts, a feeding hole corresponds to a suction device, a discharging hole is provided with a constant temperature incubator, one rotating shaft of the first conveying belt extends out of the fifth vertical plate and is sleeved with one end of a second sleeve, and a bolt is arranged at the sleeving position, the other end of the second sleeve is in transmission connection with a fourth transmission rod which is rotatably arranged on the side wall of one of the fifth vertical plates through a mounting piece through a fifth bevel gear set, and the fourth transmission rod is in transmission connection with an output shaft of a third motor through a first belt.

As a further scheme of the present invention, the separation unit includes a sixth vertical plate, a seventh vertical plate, a fifth mounting plate, a third chute, a first sliding plate, a third gear, a rack, a second cylinder, a fourth motor, a sixth bevel gear set, a sixth through groove, a perforating cylinder, a test tube rack, a rotary table, a top plate, a fourth chute, a tenth spring, a seventh through groove, an eleventh spring, a second sliding plate, a top rod, a second cam, a second transmission belt, a fifth transmission rod, a third sleeve, a fourth sleeve, a second belt, a sixth bevel gear set, a seventh bevel gear set, an eighth bevel gear set, and a sixth transmission rod;

the third chute is fixed between two sixth vertical plates which are oppositely arranged, the first sliding plate is arranged on the upper end surface of the third chute in a sliding manner, the second air cylinder and the fourth motor are fixed on the upper end surface of the first sliding plate, an output shaft of the fourth motor penetrates through the first sliding plate, is positioned in the third chute and is fixedly provided with a third gear, a rack which is meshed with the third gear is fixed in the third chute along the length direction of the third chute, the telescopic end of the second air cylinder penetrates through the first sliding plate and penetrates out of the third chute through a sixth through groove which is arranged on a floor of the third chute, a punching cylinder is fixed in the third chute, a top plate is movably arranged in the punching cylinder, the top plate is connected with the inner wall of the punching cylinder through a tenth spring, the side wall of the punching cylinder is provided with the fourth chute, a push rod which is arranged in the fourth chute in a sliding manner is fixed on the top plate, and a seventh through groove is arranged on one of the sixth vertical plates, the second sliding plate is arranged in the seventh through groove in a sliding manner, one end of the second sliding plate extends out of the seventh through groove to be in sliding contact with the convex part of the second cam, the other end of the second sliding plate extends out of the seventh through groove to be fixedly provided with a push rod matched with the push rod, a seventh vertical plate is also oppositely arranged between the two oppositely arranged sixth vertical plates, a second conveying belt is rotatably arranged between the seventh vertical plate and one of the sixth vertical plates, the test tube rack is rotatably arranged on the second conveying belt, a plurality of test tubes are arranged in the test tube rack along the movement direction of the second conveying belt, the test tube rack is positioned below the push rod, a fifth mounting plate is fixedly arranged between the seventh vertical plate and the other sixth vertical plate, a rotating disc is rotatably arranged on the fifth mounting plate and positioned below the third sliding groove, one end of a fifth driving rod is fixed on the lower end face of the rotating disc, the other end of the fifth driving rod is rotatably connected with one end of the sixth driving rod through a sixth bevel gear set through the fifth mounting plate, the other end of the sixth transmission rod is rotatably sleeved with one end of a third sleeve, a bolt is arranged at the sleeved position, the other end of the third sleeve is in transmission connection with an output shaft of a third motor through a seventh bevel gear set, one end of a fourth sleeve is in transmission connection with the output shaft of the third motor through a second belt, the other end of the fourth sleeve is rotatably sleeved with one rotating shaft of a second transmission belt, the bolt is arranged at the sleeved position, a second cam is fixed at one end of a rotating rod rotatably arranged on one of sixth vertical plates, and the other end of the rotating rod is in transmission connection with the output shaft of the fourth motor through an eighth bevel gear set.

As a further aspect of the present invention, the storage unit includes a low temperature storage box and a third cylinder, the low temperature storage box is mounted on the upper end surface of the fifth mounting plate, a feed port of the low temperature storage box corresponds to a discharge port of the second conveyor belt, the third cylinder is fixed on one of the sixth vertical plates through a mounting member, and a telescopic end of the third cylinder corresponds to a feed port of the low temperature storage box.

After adopting the structure, compared with the prior art, the invention has the advantages that:

1. according to the invention, the opening of the mixing cylinder can be sealed in the process of vertical vibration and mixing through the sealing cover plate capable of moving up and down to prevent the omission of the solution in the vibration process, the through hole in the sealing cover plate is clamped with the second slide bar by pressing the sealing cover plate downwards, the first cam drives the vibration plate to vibrate up and down under the action of the first motor so as to drive the mixing tube to vibrate up and down, the mixing tube is slidably sleeved with the positioning cylinder so as to replace and disassemble the mixing cylinder, after mixing, the first fixed plate and the second fixed plate are moved in the far direction by pressing the first push plate to compress the first telescopic rod, so that the second slide bar is separated from the through hole, the sealing cover plate is separated from the mixing cylinder under the action of the reset of the second spring, and the telescopic end of the first electric telescopic rod is stretched to drive the positioning cylinder to slide on the upper end face of the vibration plate.

2. According to the invention, the sucking device which is rotatably arranged on the movable plate is driven by the first threaded rod and the first threaded sleeve to move up and down so as to transfer the solution in the mixing cylinder to the culture dish, and the strickling rod can strickle off the strain-containing solution on the upper surface of the culture medium directly sown by the culture dish on the surface of the culture medium uniformly under the rotating action of the sucking device, so that the strain grows uniformly in the culture process, the yield is high, and the structure is simple.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural diagram of the vibration unit of the present invention.

Fig. 3 is a top view of the vibration unit of the present invention.

Fig. 4 is a cross-sectional view a-a of fig. 3.

Fig. 5 is a schematic structural view of the first fixing plate and the second fixing plate of the present invention.

Fig. 6 is a schematic structural view of the extraction unit of the present invention.

FIG. 7 is a schematic diagram of the structure of the inoculating unit of the present invention.

Figure 8 is a top view of a tapping unit and an inoculating unit of the present invention.

Fig. 9 is a sectional view of B-B in fig. 8.

FIG. 10 is a schematic view of the structure of the separation unit of the present invention.

Fig. 11 is a schematic structural view of the turntable of the present invention.

FIG. 12 is a top view of a separation unit of the present invention.

Fig. 13 is a cross-sectional view of C-C in fig. 12.

In the figure: 1-a first mounting plate; 2-a vibration plate; 3-a first slide bar; 4-a first spring; 5-a first electric telescopic rod; 6, positioning a cylinder; 7-a mixing tube; 8-sealing the cover plate; 9-a first chute; 10-a first slider; 11-a second spring; 12-a first riser; 13-a first cam; 14-a first electric machine; 15-a first through slot; 16-a second through slot; 17-a first telescopic rod; 18-a first fixing plate; 19-a second fixing plate; 20-a first sleeve; 21-a second slide bar; 22-a third spring; 23-positioning holes; 24-a second telescoping rod; 25-a fourth spring; 26-a fifth spring; 27-a first push plate; 28-a first hinge lever; 29-a second articulation bar; 30-a second mounting plate; 31-a second riser; 32-a third through slot; 33-a second electric telescopic rod; 34-a third mounting plate; 35-moving the plate; 36-a dip vessel; 37-a piston plate; 38-dip tube; 39-a scraping rod; 40-a connecting plate; 41-a first threaded rod; 42-a first threaded sleeve; 43-a second motor; 44-a first bevel gear set; 45-second bevel gear set; 46-a third bevel gear set; 47-a first gear; 48-a second gear; 49-first transfer lever; 50-a second drive link; 51-a third drive link; 52-a third riser; 53-a fourth mounting plate; 54-a fourth riser; 55-a first baffle; 56-a second push plate; 57-a third slide bar; 58-sixth spring; 59-culture dish; 60-a second chute; 61-a fourth through slot; 62-U-shaped slides; 63-connecting rods; 64-a second threaded rod; 65-a second threaded sleeve; 66-a second baffle; 67-a fourth bevel gear set; 68-a third motor; 69-a fifth vertical plate; 70-mounting the box; 71-a fourth slide bar; 72-a seventh spring; 73-a third push plate; 74-a fixed mount; 75-a via; 76-a fifth slide bar; 77-eighth spring; 78-a sixth slide bar; 79-ninth spring; 80-hinged plate; 81-fifth through slot; 82-a first cylinder; 83-L shaped push plate; 84-a first conveyor belt; 85-a first belt; 86-a second sleeve; 87-fourth transmission rod; 88-culture dish cover; 89-a fifth bevel gear set; 90-a sixth vertical plate; 91-a seventh riser; 92-a fifth mounting plate; 93-a third chute; 94-a first slide plate; 95-third gear; 96-rack; 97-a second cylinder; 98-a fourth motor; 99-sixth bevel gear set; 100-a sixth through slot; 101-a perforating cylinder; 102-test tubes; 103-test tube rack; 104-a turntable; 105-a top plate; 106-a fourth chute; 107-tenth spring; 108-a seventh through slot; 109-eleventh spring; 110-a second sled; 111-a top rod; 112-a second cam; 113-a second conveyor belt; 114-a fifth transmission rod; 115-a third sleeve; 116-a fourth sleeve; 117-a second belt; 118-a sixth bevel gear set; 119-a seventh bevel gear set; 120-an eighth bevel gear set; 121-a sixth transmission rod; 122-a cryopreservation tank; 123-third cylinder.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

In addition, an element of the present invention may be said to be "fixed" or "disposed" to another element, either directly on the other element or with intervening elements present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.

As shown in FIGS. 1 to 13, in the embodiment of the present invention, an apparatus for separating and purifying a biosurfactant-producing strain comprises a vibration unit capable of vibrating up and down, a suction unit capable of moving up and down, an inoculation unit, a sealing movement unit, a separation unit, and a preservation unit;

In the embodiment of the invention, the vibration unit comprises a first mounting plate 1, a vibration plate 2, a first slide rod 3, a first spring 4, a first electric telescopic rod 5, a positioning cylinder 6, a mixing pipe 7, a sealing cover plate 8, a first chute 9, a first slide block 10, a second spring 11, a first vertical plate 12, a first cam 13, a first motor 14 and a clamping assembly;

a plurality of first slide bars 3 are fixed on the upper end surface of the first mounting plate 1, one ends of the first slide bars 3 far away from the first mounting plate 1 all slide through the vibration plate 2 and are fixed with a limiting block, a plurality of first springs 4 are sleeved on the outer wall of the first slide bars 3, one ends of the first springs 4 are fixed on the upper end surface of the first mounting plate 1, the other ends are fixed on the lower end surface of the vibration plate 2, a positioning cylinder 6 is fixed on the upper end surface of the vibration plate 2, the lower end of the mixing tube 7 is slidably sleeved in the positioning cylinder 6, the upper end of the mixing tube is positioned on the lower end surface of the sealing cover plate 8, the first vertical plate 12 is vertically fixed on the upper end surface of the vibration plate 2, a first slide groove 9 is arranged on the side wall close to the positioning cylinder 6 along the up-down direction, one end of the first slide block 10 is slidably arranged in the first slide groove 9, and the other end extends out of the first slide groove 9 to be fixed with the sealing cover plate 8, the improved structure of the vibrating plate is characterized in that a second spring 11 is arranged in the sealing cover plate 8, one end of the second spring 11 is fixed to the lower end face of the sealing cover plate 8, the other end of the second spring 11 is fixed to the inner wall of the first sliding groove 9, the first electric telescopic rod 5 is fixed to the side wall of the first vertical plate 12, the telescopic end of the first electric telescopic rod is far away from the first vertical plate 12 and is fixed to the outer wall of the positioning cylinder 6, the first motor 14 is fixed to the upper end face of the first mounting plate 1, a first cam 13 is fixed to an output shaft of the first electric telescopic rod, the protruding portion of the first cam 13 is in sliding contact with the lower end face of the vibrating plate 2, and a clamping assembly used for locking the opening ends of the sealing cover plate 8 and the mixing pipe 7 is arranged on the first vertical plate 12.

In the embodiment of the present invention, the clamping assembly includes a first through slot 15, a second through slot 16, a first telescopic rod 17, a first fixing plate 18, a second fixing plate 19, a first sleeve 20, a second sliding rod 21, a third spring 22, a positioning hole 23, a second telescopic rod 24, a fourth spring 25, a fifth spring 26, a first push plate 27, a first hinge rod 28, and a second hinge rod 29;

the first vertical plate 12 is provided with a first through groove 15 and a second through groove 16 which are oppositely arranged, a first fixing plate 18 and a second fixing plate 19 are respectively arranged in the first through groove 15 and the second through groove 16 in a sliding manner, the positioning cylinder 6 is positioned between the first fixing plate 18 and the second fixing plate 19, a second sliding rod 21 is respectively arranged on the opposite surface of the first fixing plate 18 and the second fixing plate 19 in a sliding manner, the adjacent ends of the two second sliding rods 21 are respectively set to be arc-shaped, the other ends of the two second sliding rods respectively penetrate through the first fixing plate 18 and the second fixing plate 19 in a sliding manner and are respectively sleeved with a first sleeve 20, the two first sleeves 20 are respectively fixed on the side walls of the first fixing plate 18 and the second fixing plate 19, a third spring 22 is respectively arranged in the two first sleeves 20, one end of the third spring 22 is fixed on the inner wall of the first sleeve 20, and the other end of the third spring is fixed at one end of the second sliding rod 21 which is sleeved with the first sleeve 20 in a sliding manner, two adjacent side walls of the sealing cover plate 8, the first fixing plate 18 and the second fixing plate 19 are respectively provided with a positioning hole 23 with an arc-shaped end clamped with the second slide bar 21, one ends of the first fixing plate 18 and the second fixing plate 19 which are respectively arranged in the first through groove 15 and the second through groove 16 in a sliding manner are respectively hinged with one ends of a first hinge rod 28 and a second hinge rod 29, the other ends of the first hinge rod 28 and the second hinge rod 29 are respectively hinged on the side wall of the first push plate 27, the first telescopic rod 17 is fixed on the side wall of the first vertical plate 12, the telescopic end of the first telescopic rod is fixed on the side wall of the first hinge rod 28 and the second hinge rod 29 hinged on the push plate 27, the fourth spring 25 is sleeved on the first telescopic rod 17, one end of the fourth spring is fixed with the side wall of the first vertical plate 12, the other end of the fourth spring is fixed with the side wall of the first push plate 27, and the second telescopic rods 24 are respectively fixed in the first through groove 15 and the second through groove 16, the telescopic ends of the two second telescopic rods 24 are respectively fixed with the side walls of the first fixing plate 18 and the second fixing plate 19, and the outer wall of each of the two second telescopic rods 24 is sleeved with a fifth spring 26.

In the embodiment of the present invention, the drawing unit includes a second mounting plate 30, a second vertical plate 31, a third through slot 32, a second electric telescopic rod 33, a third mounting plate 34, a moving plate 35, a drawing device 36, a piston plate 37, a drawing tube 38, a scraping rod 39, a connecting plate 40, a first threaded rod 41, a first threaded sleeve 42, a second motor 43, a first bevel gear set 44, a second bevel gear set 45, a third bevel gear set 46, a first gear 47, a second gear 48, a first transmission rod 49, a second transmission rod 50, a third transmission rod 51, a third vertical plate 52, and a fourth mounting plate 53;

the second vertical plate 31 and the third vertical plate 52 are oppositely arranged and fixed on the upper end surface of the second mounting plate 30, the second vertical plate 31 is provided with a third through groove 32 along the vertical direction, the fourth mounting plate 53 is positioned above the second mounting plate 30 and fixed between the second vertical plate 31 and the third vertical plate 52, one end of the connecting plate 40 is rotatably provided with a first threaded sleeve 42, the other end of the connecting plate slides through the third through groove 32 and is fixed with a moving plate 35, the moving plate 35 is rotatably provided with a dip device 36, a piston plate 37 is movably arranged in the dip device 36, the third mounting plate 34 is fixed on the upper end surface of the moving plate 35 through a mounting frame, the second electric telescopic rod 33 is fixed on the lower end surface of the third mounting plate 34, the telescopic end of the second electric telescopic rod passes through the upper end of the dip device 36 in a sealing and sliding manner and is fixed with the piston plate 37, the lower end of the dip device 36 is communicated with the upper end of the dip tube 38, the outer wall of the dip device 36 is further sleeved with a first gear 47, the scraping rod 39 is horizontally arranged right below the lower end of the dip tube 38 and is rotationally connected with the outer wall of the dip device 36 through a support rod, one end of a first threaded rod 41 is rotationally arranged on the upper end face of the second mounting plate 30, the other end of the first threaded rod rotates to penetrate through a fourth mounting plate 53 and is in transmission connection with an output shaft of a second motor 43 fixed on the upper end face of the fourth mounting plate 53 through a first bevel gear set 44, the first threaded rod 41 is in threaded fit with a first threaded sleeve 42, an output shaft of the second motor 43 is in rotational fit with one end of a first transmission rod 49, a bolt is arranged at the fit position, the other end of the first transmission rod 49 is in transmission connection with one end of a second transmission rod 50 through a second bevel gear set 45, the other end of the second transmission rod 50 is in transmission connection with one end of a third transmission rod 51 through a third bevel gear set 46, and a second gear 48 meshed with a first gear 47, the scooper 36 is located on the stroke of the positioning cylinder 6 moving left and right.

In the embodiment of the present invention, the inoculation unit includes a fourth vertical plate 54, a first baffle 55, a second push plate 56, a third slide rod 57, a sixth spring 58, a culture dish 59, a second chute 60, a fourth through groove 61, a U-shaped sliding plate 62, a connecting rod 63, a second threaded rod 64, a second threaded sleeve 65, a second baffle 66, a fourth bevel gear set 67, and a third motor 68;

the first baffle 55 and the third vertical plate 52 are oppositely arranged and fixed on the upper end surface of the second mounting plate 30, two oppositely arranged fourth vertical plates 54 are arranged between the first baffle 55 and the third vertical plate 52, the second push plate 56 is slidably arranged on the upper end surface of the second mounting plate 30 and is positioned between the oppositely arranged fourth vertical plates 54, one end of a third sliding rod 57 is fixed on one side wall of the second push plate 56, the other end of the third sliding rod 57 is slidably sleeved with a sixth spring 58 and slidably penetrates through the third vertical plate 52, one end of the sixth spring 58 is fixed on the side wall of the second push plate 56, the other end of the sixth spring is fixed on the side wall of the third vertical plate 52, the other side wall of the second push plate 56 is in contact with the outer wall of a culture dish 59, a plurality of culture dishes 59 can be arranged between the two oppositely arranged fourth vertical plates 54 along the sliding direction of the third sliding rod 57, and the culture dishes 59 are slidably arranged on the upper end surface of the second mounting plate 30, a second chute 60 is formed in the upper end surface of the second mounting plate 30 along a sliding direction perpendicular to the third sliding rod 57, a fourth through groove 61 is further formed in the second chute 60, the U-shaped sliding plate 62 is slidably disposed in the second chute 60, an opening of the U-shaped sliding plate is communicated with a channel between two oppositely disposed fourth vertical plates 54, the scooper 36 is located on a stroke of left and right movement of the U-shaped sliding plate 62, one end of the connecting rod 63 is fixed to the lower end surface of the U-shaped sliding plate 62, the other end of the connecting rod slides through the fourth through groove 61 and is fixed with a second threaded sleeve 65, two ends of the second threaded rod 64 are respectively rotatably disposed on two support plates fixed to the lower end surface of the second mounting plate 30, the second threaded rod 64 is threadedly engaged with the second threaded sleeve 65, the second threaded rod is further in transmission connection with an output shaft of a third motor 68 through a fourth bevel gear set 67, and a through-mounting member is fixed to one of the U-shaped sliding plate 62 for opening A second baffle 66 of the track;

after the culture dish 59 is pushed onto the U-shaped sliding plate 62 by the second pushing plate 56, the U-shaped sliding plate 62 drives the culture dish 59 to slide in the second sliding groove 60 and drives the second baffle 66 to move, so as to baffle the next culture dish 59 between the two fourth vertical plates 54, so that the next culture dish 59 enters the U-shaped sliding plate 62 after the U-shaped sliding plate 62 is reset.

In the embodiment of the present invention, the sealing moving unit includes a fifth vertical plate 69, a mounting box 70, a fourth sliding rod 71, a seventh spring 72, a third push plate 73, a fixing frame 74, a through hole 75, a fifth sliding rod 76, an eighth spring 77, a sixth sliding rod 78, a ninth spring 79, a hinge plate 80, a fifth through groove 81, a first cylinder 82, an L-shaped push plate 83, a first conveyor belt 84, a first belt 85, a second sleeve 86, a fourth conveyor belt 87, a culture dish cover 88, and a fifth bevel gear set 89;

the mounting box 70 is fixed on the upper end surfaces of two fifth vertical plates 69 which are arranged oppositely, a third push plate 73 is movably arranged in the mounting box 70, one side wall of the third push plate 73 is connected with one end of a fourth slide bar 71, the other end of the fourth slide bar 71 is sleeved with a seventh spring 72 and slides out of the side wall of the mounting box 70, one end of the seventh spring 72 is fixed on the side wall of the third push plate 73, the other end of the seventh spring is fixed on the inner wall of the mounting box 70, the other side wall of the third push plate 73 is contacted with the outer wall of a culture dish cover 88, a through hole 75 matched with the culture dish cover 88 is arranged on the bottom plate of the mounting box 70, a fifth slide bar 76 is arranged on the two fifth vertical plates 69 which are arranged oppositely in a sliding manner, one end of the two fifth slide bars 76 is positioned under the through hole 75, the other end of the two fifth slide bars 76 respectively slides through the fifth vertical plates 69 and is sleeved with an eighth spring 77, one end of the eighth spring 77 is fixed with a stopper fixed at one end of the fifth slide bar 76, the other end is fixed with the side wall of a fifth vertical plate 69, the hinged plate 80 is hinged on the upper end surface of the mounting box 70, one end of the hinged plate extends out of the mounting box 70, the other end is positioned on the upper end surface of the mounting box 70 and is provided with a fifth through groove 81, one end of a sixth slide bar 78 penetrates through a fixed frame 74 fixed on the upper end surface of the mounting box 70 in a sliding manner, the other end is fixed with a limit plate and penetrates through the mounting box 70 in a sliding manner through the fifth through groove 81 and is positioned in a through hole 75, a ninth spring 79 is sleeved on the sixth slide bar 78, one end of the ninth spring is fixed on the lower end surface of the fixed frame 74, the other end of the ninth spring is fixed on the upper end surface of the limit plate, a first air cylinder 82 is fixed on the upper end surface of the second mounting plate 30, the telescopic end of the first air cylinder is fixed with an L-shaped push plate 83, the short plate of the L-shaped push plate 83 is in sliding fit with one end of the hinged plate 80 extending out of the mounting box 70, the first conveying belt 84 is rotatably arranged between the two oppositely arranged fifth vertical plates 69 through two rotating shafts, the feeding hole of the first transmission belt 84 corresponds to the dip extractor 36, the discharging hole is provided with a constant temperature incubator, one rotating shaft of the first transmission belt 84 extends out of the fifth vertical plate 69 and is sleeved with one end of the second sleeve 86, a bolt is arranged at the sleeved position, the other end of the second sleeve 86 is in transmission connection with a fourth transmission rod 87 which is rotatably arranged on the side wall of one of the fifth vertical plates 69 through a mounting piece through a fifth bevel gear set 89, and the fourth transmission rod 87 is in transmission connection with an output shaft of the third motor 68 through the first belt 85.

In the embodiment of the present invention, the separating unit includes a sixth vertical plate 90, a seventh vertical plate 91, a fifth mounting plate 92, a third chute 93, a first sliding plate 94, a third gear 95, a rack 96, a second cylinder 97, a fourth motor 98, a sixth bevel gear set 99, a sixth through groove 100, a punching cylinder 101, a test tube 102, a test tube rack 103, a turntable 104, a top plate 105, a fourth chute 106, a tenth spring 107, a seventh through groove 108, an eleventh spring 109, a second sliding plate 110, a top rod 111, a second cam 112, a second conveyor belt 113, a fifth transmission rod 114, a third sleeve 115, a fourth sleeve 116, a second belt 117, a sixth bevel gear set 118, a seventh bevel gear set 119, an eighth bevel gear set 120, and a sixth transmission rod 121;

the third sliding chute 93 is fixed between two oppositely arranged sixth vertical plates 90, the first sliding plate 94 is slidably arranged on the upper end surface of the third sliding chute 93, the second air cylinder 97 and the fourth motor 98 are fixed on the upper end surface of the first sliding plate 94, the output shaft of the fourth motor 98 passes through the first sliding plate 94, is positioned in the third sliding chute 93 and is fixed with a third gear 95, a rack 96 meshed with the third gear 95 is fixed in the third sliding chute 93 along the length direction, the telescopic end of the second air cylinder 97 passes through the first sliding plate 94 and passes through a sixth through groove 100 arranged on the floor of the third sliding chute 93 to penetrate out of the third sliding chute 93 to be fixed with a punching barrel 101, a top plate 105 is movably arranged in the punching barrel 101, the top plate 105 is connected with the inner wall of the punching barrel 101 through a tenth spring 107, a fourth sliding chute 106 is arranged on the side wall of the punching barrel 101, and a push rod slidably arranged in the fourth sliding chute 106 is fixed on the top plate 105, one of the sixth vertical plates 90 is provided with a seventh through groove 108, the second sliding plate 110 is slidably disposed in the seventh through groove 108, one end of the second sliding plate extends out of the seventh through groove 108 and is in sliding contact with a convex portion of the second cam 112, the other end of the second sliding plate extends out of the seventh through groove 108 and is fixed with a push rod 111 matched with the push rod, a seventh vertical plate 91 is further disposed between the two oppositely disposed sixth vertical plates 90, a second transmission belt 113 is rotatably disposed between the seventh vertical plate 91 and one of the sixth vertical plates 90, the test tube rack 103 is rotatably disposed on the second transmission belt 113, the plurality of test tubes 102 are arranged in the test tube rack 103 along the moving direction of the second transmission belt 113, the test tube rack 103 is located below the push rod 111, a fifth mounting plate 92 is fixed between the seventh vertical plate 91 and the other sixth vertical plate 90, the fifth mounting plate 92 is rotatably disposed on the fifth mounting plate 92, and the turntable 104 is located below the third through groove 93, one end of the fifth transmission rod 114 is fixed on the lower end face of the turntable 104, the other end of the fifth transmission rod passes through the fifth mounting plate 92 and is in transmission connection with one end of the sixth transmission rod 121 through the sixth bevel gear set 118, the other end of the sixth transmission rod 121 is in transmission fit with one end of the third sleeve 115, a bolt is arranged at the fit position, the other end of the third sleeve 115 is in transmission connection with an output shaft of the third motor 68 through the seventh bevel gear set 119, one end of the fourth sleeve 116 is in transmission connection with an output shaft of the third motor 68 through the second belt 117, the other end of the fourth sleeve is in transmission fit with one rotating shaft of the second transmission belt 113, a bolt is arranged at the fit position, the second cam 112 is fixed at one end of the rotating rod rotatably arranged on one of the sixth vertical plates 90, and the other end of the rotating rod is in transmission connection with an output shaft of the fourth motor 98 through the eighth bevel gear set 120.

In the embodiment of the present invention, the storage unit includes a low temperature storage box 122 and a third cylinder 123, the low temperature storage box 122 is mounted on the upper end surface of the fifth mounting plate 92, a feeding port of the low temperature storage box 122 corresponds to a discharging port of the second conveyor 113, the third cylinder 123 is fixed on one of the sixth vertical plates 90 by a mounting member, and a telescopic end of the third cylinder 123 corresponds to a feeding port of the low temperature storage box 122.

The working principle is as follows: in a sterile environment, after raw materials containing strains to be inoculated and a diluent are added into a mixing pipe 7 according to a ratio, a sealing cover plate 8 is pressed to move downwards to compress a second spring 11 through a first sliding block 10, so that a positioning hole 23 on the sealing cover plate 8 is clamped with a second sliding rod 21 to be tightly attached to an opening of the mixing pipe 7 through the sealing cover plate 8, the opening of the mixing pipe 7 is sealed, a first motor 14 is started, a first cam 13 is driven to rotate, a vibration plate 2 vibrates up and down along a first sliding rod 3 under the action of the first sliding rod 3 and a first spring 4, so that the solution in the mixing pipe 7 is uniformly mixed, a first push plate 27 is pushed to compress a fourth spring 25 after the solution in the mixing pipe 7 is uniformly mixed, and a first hinged rod 28 and a second hinged rod 29 respectively push a first fixing plate 18 and a second fixing plate 19 to respectively slide in a first through groove 15 and a second through groove 16 to compress a fifth spring 26, so as to separate the positioning hole 23 from the second sliding rod 21, the press seal cover plate 8 is reset under the action of the second spring 11, so that the press seal cover plate 8 is separated from the mixing tube 7, the first electric telescopic rod 5 is started to push the positioning cylinder 6 to drive the mixing tube 7 to slide from the upper end surface of the vibration plate 2 to the upper end surface of the second mounting plate 30 and to be positioned under the lower end of the dip tube 38, and simultaneously the outer wall close to the dip device 36 is contacted with the scraping rod 39 and pushes the scraping rod 39 to turn over, so as not to affect the dip tube 38 to move up and down in the mixing tube 7, then the second electric motor 43 is started to drive the first threaded rod 41 to rotate through the first bevel gear set 44 so as to drive the connecting plate 40 to move up and down in the third through groove 32 and drive the moving plate 35 to move up and down, when the dip tube 38 is positioned under the liquid level in the mixing tube 7, the second electric telescopic rod 33 is started to extend and retract, thereby driving the piston plate 37 to move in the dip device 36, pressure difference is generated to draw the solution in the mixing tube 7, then the moving plate 35 drives the drawing tube 38 to move upwards and away from the mixing tube 7, the mixing tube 7 is reset under the action of the first electric telescopic rod 5, the third motor 68 is started to drive the second threaded rod 64 to rotate through the fourth bevel gear set 67, so that the U-shaped sliding plate 62 is driven by the second threaded sleeve 65 to slide in the second sliding groove 60 to be under the lower end of the drawing tube 38, a plurality of the U-shaped sliding plates are arranged between the two fourth vertical plates 54, the second pushing plate 56 pushes the culture dish 59 to be clamped on the upper end face of the bottom plate of the U-shaped sliding plate 62 through the third sliding rod 57 and the sixth spring 58 so as to drive the culture dish 59 to be positioned under the lower end of the drawing tube 38, the moving plate 35 moves downwards until the scraping rod 39 is just attached to the upper end face of the culture medium in the culture dish 59, the second electric telescopic rod 33 stretches and drives the piston plate 37 to move to push the solution in the drawing device 36 out to the upper end face of the culture dish 38, at the moment, the first gear 47 and the second gear 48 are just meshed, the second gear 48 is driven to rotate by the second bevel gear set 45, the third bevel gear set 46, the first transmission rod 49, the second transmission rod 50, the third transmission rod 51 and the bolt, so that the drawer 36 is driven to rotate by the first gear 47, the scraping rod 39 is driven to rotate on the upper end surface of the culture medium, the solution is horizontally and uniformly scraped on the upper end surface of the culture medium, then the moving plate 35 moves upwards to drive the scraping rod 39 to be separated from the culture medium, the first air cylinder 82 is started, the telescopic end of the first air cylinder 82 drives the L-shaped push plate 83 to pass through the third through groove 32 to slide on the upper end surface of the second mounting plate 30, the inoculated culture dish 59 is pushed out of the U-shaped slide plate 62 to slide to the first transmission belt 84, meanwhile, the L-shaped push plate 83 is in sliding contact with one end of the hinge plate 80 away from the fifth through groove 81 in the pushing process, so as to drive the hinge plate 80 to turn over along the hinge point, the hinged plate 80 pulls out one end of the sixth sliding rod 78 positioned in the through hole 75 and compresses a ninth spring 79 under the action of the fifth through groove 81 and the stopper during overturning, the culture dish cover 88 falls into the through hole 75 under the action of the third push plate 73, the fourth sliding rod 71 and the seventh spring 72, an opening of the culture dish cover is in contact with the outer wall of the fifth sliding rod 76, when the culture dish 59 is positioned under the culture dish cover 88, the telescopic end of the first air cylinder 82 drives the L-shaped push plate 83 to reset, the sixth sliding rod 78 resets under the action of the ninth spring 79, the lower end of the sixth sliding rod pushes the culture dish cover 88 to extrude the fifth sliding rod 76 to slide on the fifth vertical plate 69, so that the culture dish cover 88 is pushed out of the through hole 75 to be sleeved with the culture dish 59 to seal the opening of the culture dish 59, and the third motor 68 drives the rotating shaft of the first transmission belt 84 to rotate through the fifth bevel gear set 89, the first belt 85, the second sleeve 86 and the fourth transmission rod 87, so that the capped culture medium is conveyed into a constant-temperature incubator for culture;

after the culture is finished, an experimenter transfers the culture dish 59 which is cultured and contains the strain to the upper end surface of a rotating disc 104, starts a fourth motor 98, drives a first sliding plate 94 to slide on the upper end of a third sliding groove 93 in a reciprocating manner through the transmission of a third gear 95 and a rack 96, moves a punching cylinder 101 to the upper part of the culture dish 59, drives the rotating disc 104 to rotate through a fifth transmission rod 114, a sixth bevel gear set 118, a sixth transmission rod 121, a bolt, a third sleeve 115 and a seventh bevel gear set 119 by the third motor 68, drives the culture dish 59 to rotate, and when the punching cylinder 101 is matched with the moving punching cylinder 101, the telescopic end of a second cylinder 97 extends out to push the punching cylinder 101 to move downwards into the culture dish 59 to punch the culture medium with the strain, retracts the telescopic end of the second cylinder 97 into the fourth motor 98 to drive the first sliding plate 94 to slide to the position right above the opening of a test tube 102, and simultaneously, the fourth motor 98 drives a second cam 112 to rotate, the convex part of the test tube rack is in contact with the second sliding plate 110, the push rod of the top plate 105 in the punching cylinder 101 and the push rod of the push rod 111 are driven to slide in the fourth sliding groove 106, the culture medium with the strains in the punching cylinder 101 is pushed into the corresponding test tubes 102, one end of each test tube 102 with the strains is conveyed to the feeding port of the low-temperature storage box 122 under the action of the second conveying belt 113, and the third air cylinder 123 is started to push the test tube rack 103 into the low-temperature storage box 122 for storage.

The foregoing is merely illustrative of the preferred embodiments of the present invention and is not to be construed as limiting the claims. The present invention is not limited to the above embodiments, and the specific structure thereof is allowed to vary. But all changes which come within the scope of the invention are intended to be embraced therein.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Claims

1. A separation and purification device of bacterial strains for preparing a biosurfactant is characterized by comprising a vibration unit capable of vibrating up and down, a drawing unit capable of moving up and down, an inoculation unit, a sealing moving unit, a separation unit and a storage unit;

2. The device for separating and purifying the bacterial strains for preparing the biosurfactant according to claim 1, wherein the vibration unit comprises a first mounting plate (1), a vibration plate (2), a first slide bar (3), a first spring (4), a first electric telescopic rod (5), a positioning cylinder (6), a mixing pipe (7), a sealing cover plate (8), a first chute (9), a first slide block (10), a second spring (11), a first vertical plate (12), a first cam (13), a first motor (14) and a clamping assembly;

a plurality of first sliding rods (3) are fixed on the upper end face of the first mounting plate (1), one ends of the first sliding rods (3) far away from the first mounting plate (1) slide through the vibration plate (2) and are fixed with limiting blocks, a plurality of first springs (4) are sleeved on the outer wall of the first sliding rods (3), one ends of the first springs (4) are fixed on the upper end face of the first mounting plate (1), the other ends of the first springs are fixed on the lower end face of the vibration plate (2), a positioning cylinder (6) is fixed on the upper end face of the vibration plate (2), the lower end of the mixing tube (7) is sleeved in the positioning cylinder (6) in a sliding manner, the upper end of the mixing tube is located on the lower end face of the sealing cover plate (8), the first vertical plate (12) is vertically fixed on the upper end face of the vibration plate (2), a first sliding groove (9) is formed in the vertical direction on the side wall of the mixing tube (6), one end of the first sliding block (10) is arranged in the first sliding manner, the other end of the first sliding groove (9) extends out of the first sliding groove to be fixed with the sealing cover plate (8), a second spring (11) is arranged in the sealing cover plate (8), one end of the second spring (11) is fixed to the lower end face of the sealing cover plate (8), the other end of the second spring is fixed to the inner wall of the first sliding groove (9), the first electric telescopic rod (5) is fixed to the side wall of the first vertical plate (12), the telescopic end of the first electric telescopic rod is far away from the first vertical plate (12) and is fixed to the outer wall of the positioning cylinder (6), the first motor (14) is fixed to the upper end face of the first mounting plate (1), a first cam (13) is fixed to an output shaft of the first electric telescopic rod, the protruding portion of the first cam (13) is in sliding contact with the lower end face of the vibration plate (2), and a clamping assembly used for locking the opening end of the.

3. The device for separating and purifying the bacterial strain for preparing the biosurfactant according to claim 2, wherein the clamping component comprises a first through groove (15), a second through groove (16), a first telescopic rod (17), a first fixing plate (18), a second fixing plate (19), a first sleeve (20), a second sliding rod (21), a third spring (22), a positioning hole (23), a second telescopic rod (24), a fourth spring (25), a fifth spring (26), a first push plate (27), a first hinge rod (28) and a second hinge rod (29);

the first vertical plate (12) is provided with a first through groove (15) and a second through groove (16) which are oppositely arranged, a first fixing plate (18) and a second fixing plate (19) are respectively arranged in the first through groove (15) and the second through groove (16) in a sliding manner, the positioning cylinder (6) is positioned between the first fixing plate (18) and the second fixing plate (19), second sliding rods (21) are respectively arranged on the opposite surfaces of the first fixing plate (18) and the second fixing plate (19) in a sliding manner, the adjacent ends of the two second sliding rods (21) are respectively in an arc shape, the other ends of the two second sliding rods respectively penetrate through the first fixing plate (18) and the second fixing plate (19) and are respectively sleeved with first sleeves (20) in a sliding manner, the two first sleeves (20) are respectively fixed on the side walls of the first fixing plate (18) and the second fixing plate (19), and third springs (22) are respectively arranged in the two first sleeves (20), one end of a third spring (22) is fixed on the inner wall of the first sleeve (20), the other end of the third spring is fixed at one end of the second sliding rod (21) which is slidably sleeved with the first sleeve (20), positioning holes (23) which are provided with arc-shaped end clamping with the second sliding rod (21) are formed in two adjacent side walls of the sealing cover plate (8), the first fixing plate (18) and the second fixing plate (19) are respectively slidably arranged in the first through groove (15) and the second through groove (16), one end of the first fixing plate (28) and one end of the second hinging rod (29) are respectively hinged with one end of the first hinging rod (28) and one end of the second hinging rod (29), the other ends of the first hinging rod (28) and the second hinging rod (29) are respectively hinged on the side wall of the first push plate (27), the first telescopic rod (17) is fixed on the side wall of the first vertical plate (12), and the telescopic end of the first telescopic rod (28) is fixed on the side wall of the push plate (27) which is hinged with the first hinging rod (28), On the lateral wall of second articulated arm (29), fourth spring (25) cover is established on first telescopic link (17), and one end is fixed with first riser (12) lateral wall, and the other end is fixed with first push pedal (27) lateral wall, first logical groove (15), second lead to all being fixed with second telescopic link (24) in groove (16), two the flexible end of second telescopic link (24) is fixed with first fixed plate (18), second fixed plate (19) lateral wall respectively, two all the cover is equipped with fifth spring (26) on second telescopic link (24) outer wall.

4. The device for separating and purifying the bacterial strains for preparing the biosurfactants, according to claim 2, is characterized in that the drawing unit comprises a second mounting plate (30), a second vertical plate (31), a third through groove (32), a second electric telescopic rod (33), a third mounting plate (34), a moving plate (35), a drawing device (36), a piston plate (37), a drawing pipe (38), a scraping rod (39), a connecting plate (40), a first threaded rod (41), a first threaded sleeve (42), a second motor (43), a first bevel gear set (44), a second bevel gear set (45), a third bevel gear set (46), a first gear (47), a second gear (48), a first transmission rod (49), a second transmission rod (50), a third transmission rod (51), a third vertical plate (52) and a fourth mounting plate (53);

the second vertical plate (31) and the third vertical plate (52) are oppositely arranged and fixed on the upper end surface of the second mounting plate (30), the second vertical plate (31) is provided with a third through groove (32) along the vertical direction, the fourth mounting plate (53) is positioned above the second mounting plate (30) and fixed between the second vertical plate (31) and the third vertical plate (52), one end of the connecting plate (40) is rotatably provided with a first thread sleeve (42), the other end of the connecting plate slidably penetrates through the third through groove (32) and is fixed with a movable plate (35), the movable plate (35) is rotatably provided with a drawing device (36), a piston plate (37) is movably arranged in the drawing device (36), the third mounting plate (34) is fixed on the upper end surface of the movable plate (35) through a mounting rack, the second electric-driven shaft (33) is fixed on the lower end surface of the third mounting plate (34), and the telescopic end of the electric-driven shaft hermetically slidably penetrates through the upper end of the drawing device (36) and is fixed with the piston plate (37), the lower end of the drawing device (36) is communicated with the upper end of the drawing tube (38), the outer wall of the drawing device (36) is further sleeved with a first gear (47), the scraping rod (39) is horizontally positioned under the lower end of the drawing tube (38) and is in rotary connection with the outer wall of the drawing device (36) through a support rod, one end of a first threaded rod (41) is rotatably arranged on the upper end face of a second mounting plate (30), the other end of the first threaded rod (41) is rotatably arranged through a fourth mounting plate (53) and is in transmission connection with an output shaft of a second motor (43) fixed on the upper end face of the fourth mounting plate (53) through a first bevel gear set (44), the first threaded rod (41) is in threaded fit with a first threaded sleeve (42), an output shaft of the second motor (43) is rotatably sleeved with one end of a first transmission rod (49), a bolt is arranged at the sleeved position, and the other end of the first transmission rod (49) is in transmission connection with one end of a second transmission rod (50) through a gear set (45), the other end of the second transmission rod (50) is in transmission connection with one end of a third transmission rod (51) through a third bevel gear set (46), a second gear (48) meshed with the first gear (47) is fixed to the other end of the third transmission rod (51), and the drawing device (36) is located on the stroke of the left and right movement of the positioning cylinder (6).

5. The device for separating and purifying the bacterial strain for preparing the biosurfactant according to claim 4, wherein the inoculation unit comprises a fourth vertical plate (54), a first baffle plate (55), a second push plate (56), a third slide bar (57), a sixth spring (58), a culture dish (59), a second sliding chute (60), a fourth through groove (61), a U-shaped sliding plate (62), a connecting rod (63), a second threaded rod (64), a second threaded sleeve (65), a second baffle plate (66), a fourth conical gear set (67) and a third motor (68);

the first baffle plate (55) and the third vertical plate (52) are oppositely arranged and fixed on the upper end surface of the second mounting plate (30), two fourth vertical plates (54) which are oppositely arranged are arranged between the first baffle plate (55) and the third vertical plate (52), the second push plate (56) is slidably arranged on the upper end surface of the second mounting plate (30) and is positioned between the fourth vertical plates (54) which are oppositely arranged, one end of a third sliding rod (57) is fixed on one side wall of the second push plate (56), the other end of the third sliding rod (57) is slidably sleeved with a sixth spring (58) and slidably penetrates through the third vertical plate (52), one end of the sixth spring (58) is fixed on the side wall of the second push plate (56), the other end of the sixth spring is fixed on the side wall of the third vertical plate (52), the other side wall of the second push plate (56) is contacted with the outer wall of a culture dish (59), and a plurality of culture dishes (59) can be arranged between the two fourth vertical plates (54) which are oppositely arranged along the sliding direction of the third sliding rod (57), the culture dish (59) is arranged on the upper end surface of the second mounting plate (30) in a sliding manner, a second sliding groove (60) is formed in the upper end surface of the second mounting plate (30) along the sliding direction perpendicular to the third sliding rod (57), a fourth through groove (61) is further formed in the second sliding groove (60), the U-shaped sliding plate (62) is arranged in the second sliding groove (60) in a sliding manner, the opening of the U-shaped sliding plate is communicated with a channel between the two oppositely arranged fourth vertical plates (54), the drawing device (36) is positioned on the left-right moving stroke of the U-shaped sliding plate (62), one end of the connecting rod (63) is fixed on the lower end surface of the U-shaped sliding plate (62), the other end of the connecting rod slides to penetrate through the fourth through groove (61) and is fixed with a second threaded sleeve (65), two ends of the second threaded rod (64) are respectively rotatably arranged on two supporting plates fixed on the lower end surface of the second mounting plate (30), and the second threaded sleeve (64) is in threaded sleeve (65), the second threaded rod is also in transmission connection with an output shaft of a third motor (68) through a fourth bevel gear set (67), and a second baffle (66) for opening and closing a channel between the two fourth vertical plates (54) is fixed on one limiting vertical plate of the U-shaped sliding plate (62) through a mounting piece.

6. The device for separating and purifying the bacterial strain for preparing the biosurfactant according to claim 5, wherein the sealing moving unit comprises a fifth vertical plate (69), a mounting box (70), a fourth sliding rod (71), a seventh spring (72), a third push plate (73), a fixing frame (74), a through hole (75), a fifth sliding rod (76), an eighth spring (77), a sixth sliding rod (78), a ninth spring (79), a hinge plate (80), a fifth through groove (81), a first cylinder (82), an L-shaped push plate (83), a first conveying belt (84), a first belt (85), a second sleeve (86), a fourth conveying rod (87), a culture dish cover (88) and a fifth bevel gear set (89);

the mounting box (70) is fixed on the upper end surfaces of two oppositely arranged fifth vertical plates (69), a third push plate (73) is movably arranged in the mounting box (70), one side wall of the third push plate (73) is connected with one end of a fourth slide bar (71), the other end of the fourth slide bar (71) is sleeved with a seventh spring (72) and slides out of the side wall of the mounting box (70), one end of the seventh spring (72) is fixed on the side wall of the third push plate (73), the other end of the seventh spring is fixed on the inner wall of the mounting box (70), the other side wall of the third push plate (73) is in contact with the outer wall of a culture dish cover (88), a through hole (75) matched with the culture dish cover (88) is formed in a bottom plate of the mounting box (70), fifth slide bars (76) are arranged on the two oppositely arranged fifth vertical plates (69) in a sliding manner, one end of each fifth slide bar (76) is positioned under the corresponding through hole (75), the other end of the eighth spring (77) respectively penetrates through the fifth vertical plate (69) in a sliding manner and is sleeved with an eighth spring (77), one end of the eighth spring (77) is fixed with a stop block fixed at one end of a fifth sliding rod (76), the other end of the eighth spring is fixed with the side wall of the fifth vertical plate (69), the hinged plate (80) is hinged on the upper end surface of the installation box (70), one end of the hinged plate extends out of the installation box (70), the other end of the hinged plate is positioned on the upper end surface of the installation box (70) and is provided with a fifth through groove (81), one end of the sixth sliding rod (78) penetrates through a fixing frame (74) fixed on the upper end surface of the installation box (70) in a sliding manner, the other end of the sixth sliding rod (78) is fixed with a limiting plate, the mounting box (70) is positioned in the through hole (75) in a sliding manner through the fifth through groove (81), the ninth spring (79) is sleeved on the sixth sliding rod (78), one end of the ninth spring is fixed on the lower end surface of the fixing frame (74), and the other end of the limiting plate is fixed on the upper end surface of the limiting plate, the first cylinder (82) is fixed on the upper end surface of the second mounting plate (30), an L-shaped push plate (83) is fixed on the telescopic end of the first cylinder, the short plate of the L-shaped push plate (83) is in sliding fit with one end of the hinge plate (80) extending out of the mounting box (70), the first conveyor belt (84) is rotatably arranged between the two fifth vertical plates (69) which are oppositely arranged through two rotating shafts, a feed port of the first conveyor belt (84) corresponds to the extractor (36), a constant temperature incubator is arranged at a discharge port, one rotating shaft of the first conveyor belt (84) extends out of the fifth vertical plate (69) and is sleeved with one end of the second sleeve (86), a bolt is arranged at the sleeving position, the other end of the second sleeve (86) is in transmission connection with a fourth transmission rod (87) which is rotatably arranged on the side wall of one of the fifth vertical plates (69) through a mounting piece through a fifth bevel gear set (89), the fourth transmission rod (87) is in transmission connection with an output shaft of the third motor (68) through a first belt (85).

7. The device for separating and purifying the bacterial strains for preparing the biosurfactants, according to claim 6, is characterized in that the separation unit comprises a sixth vertical plate (90), a seventh vertical plate (91), a fifth mounting plate (92), a third sliding groove (93), a first sliding plate (94), a third gear (95), a rack (96), a second air cylinder (97), a fourth motor (98), a sixth bevel gear set (99), a sixth through groove (100), a punching cylinder (101), a test tube (102), a test tube rack (103), a rotary table (104), a top plate (105), a fourth sliding groove (106), a tenth spring (107), a seventh through groove (108), an eleventh spring (109), a second sliding plate (110), a top rod (111), a second cam (112), a second conveying belt (113), a fifth driving rod (114), a third sleeve (115), a fourth sleeve (116), A second belt (117), a sixth bevel gear set (118), a seventh bevel gear set (119), an eighth bevel gear set (120) and a sixth transmission rod (121);

the third sliding groove (93) is fixed between two sixth vertical plates (90) which are oppositely arranged, the first sliding plate (94) is arranged on the upper end surface of the third sliding groove (93) in a sliding manner, the second air cylinder (97) and the fourth motor (98) are fixed on the upper end surface of the first sliding plate (94), an output shaft of the fourth motor (98) penetrates through the first sliding plate (94) and is positioned in the third sliding groove (93) and is fixedly provided with a third gear (95), a rack (96) which is meshed with the third gear (95) is fixed in the third sliding groove (93) along the length direction of the third sliding groove (93), the telescopic end of the second air cylinder (97) penetrates through the first sliding plate (94) and penetrates through a sixth through groove (100) formed in the floor of the third sliding groove (93) to penetrate through the third sliding groove (93) and is fixedly provided with a punching barrel (101), a top plate (105) is movably arranged in the punching barrel (101), and the top plate (105) is connected with the inner wall of the punching barrel (101) through a tenth spring (107), a fourth chute (106) is formed in the side wall of the perforating cylinder (101), a push rod which is slidably arranged in the fourth chute (106) is fixed on the top plate (105), a seventh through groove (108) is formed in one of the sixth vertical plates (90), the second sliding plate (110) is slidably arranged in the seventh through groove (108), one end of the second sliding plate extends out of the seventh through groove (108) and is in sliding contact with a protruding part of the second cam (112), the other end of the second sliding plate extends out of the seventh through groove (108) and is fixed with a push rod (111) matched with the push rod, a seventh vertical plate (91) is also oppositely arranged between the two oppositely arranged sixth vertical plates (90), a second conveying belt (113) is rotatably arranged between the seventh vertical plate (91) and one of the sixth vertical plates (90), the test tube rack (103) is rotatably arranged on the second conveying belt (113), and the test tubes (102) are arranged in the test tube rack (103) along the moving direction of the second conveying belt (113), the test tube rack (103) is positioned below the ejector rod (111), a fifth mounting plate (92) is fixed between the seventh vertical plate (91) and the other sixth vertical plate (90), a rotary table (104) is rotatably arranged on the fifth mounting plate (92), the rotary table (104) is positioned below the third sliding groove (93), one end of a fifth transmission rod (114) is fixed on the lower end face of the rotary table (104), the other end of the fifth transmission rod passes through the fifth mounting plate (92) in a rotating mode and is in transmission connection with one end of a sixth transmission rod (121) through a sixth bevel gear set (118), the other end of the sixth transmission rod (121) is in rotating fit with one end of a third sleeve (115), a bolt is arranged at the fit position, the other end of the third sleeve (115) is in transmission connection with an output shaft of a third motor (68) through a seventh bevel gear set (119), one end of a fourth sleeve (116) is in transmission connection with an output shaft of the third motor (68) through a second belt (117), the other end of the second cam (112) is rotatably sleeved with one rotating shaft of the second transmission belt (113), a bolt is arranged at the sleeved position, the second cam is fixed at one end of a rotating rod rotatably arranged on one sixth vertical plate (90), and the other end of the rotating rod is in transmission connection with an output shaft of a fourth motor (98) through an eighth bevel gear set (120).

8. The device for separating and purifying a biosurfactant-producing strain according to claim 7, wherein the storage unit comprises a low-temperature storage box (122) and a third cylinder (123), the low-temperature storage box (122) is mounted on the upper end face of the fifth mounting plate (92), the feeding port of the low-temperature storage box corresponds to the discharging port of the second conveyor belt (113), and the third cylinder (123) is fixed on one of the sixth vertical plates (90) through a mounting piece, and the telescopic end of the third cylinder corresponds to the feeding port of the low-temperature storage box (122).